CsGy3G017770 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy3G017770
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionaspartic proteinase PCS1
LocationGy14Chr3: 13730786 .. 13732382 (-)
RNA-Seq ExpressionCsGy3G017770
SyntenyCsGy3G017770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAACCCATCAGCAACCCCCTCCCCACAATCAACAATGCTTCTGATTCTCTTCTCTCTCTCCTTATTCACTCTCTCCTTCTCTCAATCCAATTCCCTCTCTCTCCCCTTCCCTCTTTCTCTCTCTGAAAAACCCTCCAATACTATCCCATCATACTCTTCCCAGCTTTACGCCAAGAGGCCATCCTCCTACGGCTCCTTCAAGCTTCCTTTCAAATACTCCTCCACCGCCCTCGTCGTCTCTCTACCGATCGGAACGCCGCCACAGCCCACTGACTTGGTTCTAGACACCGGCAGCCAACTCTCTTGGATTCAATGTCATGACAAAAAGATTAAGAAAAGATTGCCCCCCTTGCCCAAGCCTAAAACCACCTCCTTTGATCCTTCTCTCTCCTCTTCTTTTTCTCTCCTCCCTTGTAATCACCCCATCTGCAAACCCCGAATTCCAGATTTTACCCTTCCCACTTCTTGTGACCAAAATCGCCTCTGCCACTACTCCTACTTCTACGCTGACGGTACCTTGGCTGAGGGAAATCTCGTCAGAGAAAAATTTACCTTCTCTAAATCCCTTTCTACCCCTCCTGTCATCCTCGGTTGCGCTCAAGCCTCCACCGAAAACAGGGGTATTTTGGGAATGAACCGTGGACGTTTGTCCTTTATCTCCCAAGCTAAAATCTCCAAATTCTCCTATTGCGTTCCGAGTCGAACCGGGTCTAATCCCACCGGGCTATTCTACCTCGGAGATAACCCCAATTCTTCCAAATTCAAATACGTCACCATGTTGACTTTTCCTGAAAGTCAAAGCTCTCCGAATCTCGACCCACTGGCTTACACTCTCCCTATGAAGGCAATAAAAATAGCCGGAAAACGGCTAAACGTCCCCCCAGCCGCTTTCAAACCGGACGCTGGTGGGTCGGGTCAAACCATGATTGACTCCGGTTCGGACCTGACTTATTTAGTGGATGAAGCGTATGAGAAGGTTAAAGAGGAGGTAGTGAGATTAGTGGGTGCGATGATGAAGAAAGGCTACGTATACGCCGACGTAGCCGACATGTGTTTCGACGCCGGTGTCACGGCGGAGGTGGGCCGCAGGATTGGCGGCATCTCGTTTGAGTTTGATAACGGAGTGGAGATTTTCGTGGGGAGAGGAGAAGGGGTTTTGACGGAAGTGGAAAAAGGAGTGAAGTGTGTGGGGATCGGACGGTCAGAAAGGCTTGGGATTGGGAGTAATATAATCGGGACTGTTCATCAACAGAATATGTGGGTGGAGTATGATTTGGCCAATAAGAGAGTAGGGTTTGGTGGAGCTGAGTGTAGCAGATTGAAGTGATGATGGGCGGTAAAGATTTATACACGTGTGTGGTTTTTGGATGTTTATATAATCATATTTGATATTGTGTTATTGTGTAAATGTGTGTATAGTTATTTCATTTCATATATATACATTCTATATAAATAAAACAAATTTTGTTCTATCCTGTAAAAATGGTTACTATCAGCTAAGTTCATAAGATGTGAATTATAATTCTAACAAAATAACATCTTCAAACATTTCAAAAGGTTTTATTACATTTCAAACAATTAATAGTAAGTTGGTCC

mRNA sequence

AAAACCCATCAGCAACCCCCTCCCCACAATCAACAATGCTTCTGATTCTCTTCTCTCTCTCCTTATTCACTCTCTCCTTCTCTCAATCCAATTCCCTCTCTCTCCCCTTCCCTCTTTCTCTCTCTGAAAAACCCTCCAATACTATCCCATCATACTCTTCCCAGCTTTACGCCAAGAGGCCATCCTCCTACGGCTCCTTCAAGCTTCCTTTCAAATACTCCTCCACCGCCCTCGTCGTCTCTCTACCGATCGGAACGCCGCCACAGCCCACTGACTTGGTTCTAGACACCGGCAGCCAACTCTCTTGGATTCAATGTCATGACAAAAAGATTAAGAAAAGATTGCCCCCCTTGCCCAAGCCTAAAACCACCTCCTTTGATCCTTCTCTCTCCTCTTCTTTTTCTCTCCTCCCTTGTAATCACCCCATCTGCAAACCCCGAATTCCAGATTTTACCCTTCCCACTTCTTGTGACCAAAATCGCCTCTGCCACTACTCCTACTTCTACGCTGACGGTACCTTGGCTGAGGGAAATCTCGTCAGAGAAAAATTTACCTTCTCTAAATCCCTTTCTACCCCTCCTGTCATCCTCGGTTGCGCTCAAGCCTCCACCGAAAACAGGGGTATTTTGGGAATGAACCGTGGACGTTTGTCCTTTATCTCCCAAGCTAAAATCTCCAAATTCTCCTATTGCGTTCCGAGTCGAACCGGGTCTAATCCCACCGGGCTATTCTACCTCGGAGATAACCCCAATTCTTCCAAATTCAAATACGTCACCATGTTGACTTTTCCTGAAAGTCAAAGCTCTCCGAATCTCGACCCACTGGCTTACACTCTCCCTATGAAGGCAATAAAAATAGCCGGAAAACGGCTAAACGTCCCCCCAGCCGCTTTCAAACCGGACGCTGGTGGGTCGGGTCAAACCATGATTGACTCCGGTTCGGACCTGACTTATTTAGTGGATGAAGCGTATGAGAAGGTTAAAGAGGAGGTAGTGAGATTAGTGGGTGCGATGATGAAGAAAGGCTACGTATACGCCGACGTAGCCGACATGTGTTTCGACGCCGGTGTCACGGCGGAGGTGGGCCGCAGGATTGGCGGCATCTCGTTTGAGTTTGATAACGGAGTGGAGATTTTCGTGGGGAGAGGAGAAGGGGTTTTGACGGAAGTGGAAAAAGGAGTGAAGTGTGTGGGGATCGGACGGTCAGAAAGGCTTGGGATTGGGAGTAATATAATCGGGACTGTTCATCAACAGAATATGTGGGTGGAGTATGATTTGGCCAATAAGAGAGTAGGGTTTGGTGGAGCTGAGTGTAGCAGATTGAAGTGATGATGGGCGGTAAAGATTTATACACGTGTGTGGTTTTTGGATGTTTATATAATCATATTTGATATTGTGTTATTGTGTAAATGTGTGTATAGTTATTTCATTTCATATATATACATTCTATATAAATAAAACAAATTTTGTTCTATCCTGTAAAAATGGTTACTATCAGCTAAGTTCATAAGATGTGAATTATAATTCTAACAAAATAACATCTTCAAACATTTCAAAAGGTTTTATTACATTTCAAACAATTAATAGTAAGTTGGTCC

Coding sequence (CDS)

ATGCTTCTGATTCTCTTCTCTCTCTCCTTATTCACTCTCTCCTTCTCTCAATCCAATTCCCTCTCTCTCCCCTTCCCTCTTTCTCTCTCTGAAAAACCCTCCAATACTATCCCATCATACTCTTCCCAGCTTTACGCCAAGAGGCCATCCTCCTACGGCTCCTTCAAGCTTCCTTTCAAATACTCCTCCACCGCCCTCGTCGTCTCTCTACCGATCGGAACGCCGCCACAGCCCACTGACTTGGTTCTAGACACCGGCAGCCAACTCTCTTGGATTCAATGTCATGACAAAAAGATTAAGAAAAGATTGCCCCCCTTGCCCAAGCCTAAAACCACCTCCTTTGATCCTTCTCTCTCCTCTTCTTTTTCTCTCCTCCCTTGTAATCACCCCATCTGCAAACCCCGAATTCCAGATTTTACCCTTCCCACTTCTTGTGACCAAAATCGCCTCTGCCACTACTCCTACTTCTACGCTGACGGTACCTTGGCTGAGGGAAATCTCGTCAGAGAAAAATTTACCTTCTCTAAATCCCTTTCTACCCCTCCTGTCATCCTCGGTTGCGCTCAAGCCTCCACCGAAAACAGGGGTATTTTGGGAATGAACCGTGGACGTTTGTCCTTTATCTCCCAAGCTAAAATCTCCAAATTCTCCTATTGCGTTCCGAGTCGAACCGGGTCTAATCCCACCGGGCTATTCTACCTCGGAGATAACCCCAATTCTTCCAAATTCAAATACGTCACCATGTTGACTTTTCCTGAAAGTCAAAGCTCTCCGAATCTCGACCCACTGGCTTACACTCTCCCTATGAAGGCAATAAAAATAGCCGGAAAACGGCTAAACGTCCCCCCAGCCGCTTTCAAACCGGACGCTGGTGGGTCGGGTCAAACCATGATTGACTCCGGTTCGGACCTGACTTATTTAGTGGATGAAGCGTATGAGAAGGTTAAAGAGGAGGTAGTGAGATTAGTGGGTGCGATGATGAAGAAAGGCTACGTATACGCCGACGTAGCCGACATGTGTTTCGACGCCGGTGTCACGGCGGAGGTGGGCCGCAGGATTGGCGGCATCTCGTTTGAGTTTGATAACGGAGTGGAGATTTTCGTGGGGAGAGGAGAAGGGGTTTTGACGGAAGTGGAAAAAGGAGTGAAGTGTGTGGGGATCGGACGGTCAGAAAGGCTTGGGATTGGGAGTAATATAATCGGGACTGTTCATCAACAGAATATGTGGGTGGAGTATGATTTGGCCAATAAGAGAGTAGGGTTTGGTGGAGCTGAGTGTAGCAGATTGAAGTGA

Protein sequence

MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFKYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTTSFDPSLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLSTPPVILGCAQASTENRGILGMNRGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNSSKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLNVPPAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGAECSRLK*
Homology
BLAST of CsGy3G017770 vs. ExPASy Swiss-Prot
Match: Q9LZL3 (Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 1.0e-62
Identity = 159/446 (35.65%), Postives = 232/446 (52.02%), Query Frame = 0

Query: 6   FSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFKYSSTA 65
           FS S F+   S S+S +L  PL     P++            RP+     KL F ++ T 
Sbjct: 32  FSFSSFS---SSSSSQTLVLPLKTRITPTD-----------HRPTD----KLHFHHNVT- 91

Query: 66  LVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTTSFDPSLSSSFSLL 125
           L V+L +GTPPQ   +V+DTGS+LSW++C+           P P   +FDP+ SSS+S +
Sbjct: 92  LTVTLTVGTPPQNISMVIDTGSELSWLRCNRSS-------NPNP-VNNFDPTRSSSYSPI 151

Query: 126 PCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLSTPPVIL 185
           PC+ P C+ R  DF +P SCD ++LCH +  YAD + +EGNL  E F F  S +   +I 
Sbjct: 152 PCSSPTCRTRTRDFLIPASCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTNDSNLIF 211

Query: 186 GC--------AQASTENRGILGMNRGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDN 245
           GC         +  T+  G+LGMNRG LSFISQ    KFSYC+ S T   P G   LGD 
Sbjct: 212 GCMGSVSGSDPEEDTKTTGLLGMNRGSLSFISQMGFPKFSYCI-SGTDDFP-GFLLLGD- 271

Query: 246 PNSSKFKYVTMLTFPE----SQSSPNLDPLAYTLPMKAIKIAGKRLNVPPAAFKPDAGGS 305
              S F ++T L +      S   P  D +AYT+ +  IK+ GK L +P +   PD  G+
Sbjct: 272 ---SNFTWLTPLNYTPLIRISTPLPYFDRVAYTVQLTGIKVNGKLLPIPKSVLVPDHTGA 331

Query: 306 GQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMM----KKGYVYADVADMCF---DAGVT 365
           GQTM+DSG+  T+L+   Y  ++   +     ++       +V+    D+C+      + 
Sbjct: 332 GQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVYEDPDFVFQGTMDLCYRISPVRIR 391

Query: 366 AEVGRRIGGISFEFDNGVEIFVGRGEGVLTEV------EKGVKCVGIGRSERLGIGSNII 425
           + +  R+  +S  F+ G EI V  G+ +L  V         V C   G S+ +G+ + +I
Sbjct: 392 SGILHRLPTVSLVFE-GAEIAVS-GQPLLYRVPHLTVGNDSVYCFTFGNSDLMGMEAYVI 442

Query: 426 GTVHQQNMWVEYDLANKRVGFGGAEC 427
           G  HQQNMW+E+DL   R+G    EC
Sbjct: 452 GHHHQQNMWIEFDLQRSRIGLAPVEC 442

BLAST of CsGy3G017770 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 3.8e-41
Identity = 117/367 (31.88%), Postives = 173/367 (47.14%), Query Frame = 0

Query: 67  VVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTTSFDPSLSSSFSLLP 126
           +++L IGTP QP   ++DTGS L W QC      +         T  F+P  SSSFS LP
Sbjct: 96  LMNLSIGTPAQPFSAIMDTGSDLIWTQC------QPCTQCFNQSTPIFNPQGSSSFSTLP 155

Query: 127 CNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLSTPPVILG 186
           C+  +C+       L +    N  C Y+Y Y DG+  +G++  E  TF  S+S P +  G
Sbjct: 156 CSSQLCQ------ALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTFG-SVSIPNITFG 215

Query: 187 CAQ-----ASTENRGILGMNRGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNSS 246
           C +           G++GM RG LS  SQ  ++KFSYC+     S P+ L  LG   NS 
Sbjct: 216 CGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLL-LGSLANSV 275

Query: 247 KFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLNVPPAAFKPDA-GGSGQTMIDS 306
                       SQ      P  Y + +  + +   RL + P+AF  ++  G+G  +IDS
Sbjct: 276 TAGSPNTTLIQSSQI-----PTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDS 335

Query: 307 GSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEF 366
           G+ LTY V+ AY+ V++E +  +   +  G   +   D+CF    +     +I      F
Sbjct: 336 GTTLTYFVNNAYQSVRQEFISQINLPVVNG--SSSGFDLCFQT-PSDPSNLQIPTFVMHF 395

Query: 367 DNG-VEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRV 426
           D G +E+     E        G+ C+ +G S +   G +I G + QQNM V YD  N  V
Sbjct: 396 DGGDLEL---PSENYFISPSNGLICLAMGSSSQ---GMSIFGNIQQQNMLVVYDTGNSVV 434

BLAST of CsGy3G017770 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 2.3e-38
Identity = 119/373 (31.90%), Postives = 172/373 (46.11%), Query Frame = 0

Query: 67  VVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTTSFDPSLSSSFSLLP 126
           ++++ IGTP      ++DTGS L W QC         P      T  F+P  SSSFS LP
Sbjct: 97  LMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQP------TPIFNPQDSSSFSTLP 156

Query: 127 CNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLSTPPVILG 186
           C    C+       LP+    N  C Y+Y Y DG+  +G +  E FTF  S S P +  G
Sbjct: 157 CESQYCQ------DLPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETS-SVPNIAFG 216

Query: 187 CAQ-----ASTENRGILGMNRGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNSS 246
           C +           G++GM  G LS  SQ  + +FSYC+ S   S+P+ L  LG   +  
Sbjct: 217 CGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTL-ALGSAASG- 276

Query: 247 KFKYVTMLTFPESQSS-----PNLDPLAYTLPMKAIKIAGKRLNVPPAAFKPDAGGSGQT 306
                     PE   S      +L+P  Y + ++ I + G  L +P + F+    G+G  
Sbjct: 277 ---------VPEGSPSTTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGM 336

Query: 307 MIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDA---GVTAEVGRRI 366
           +IDSG+ LTYL  +AY  V +     +   +      +     CF     G T +V    
Sbjct: 337 IIDSGTTLTYLPQDAYNAVAQAFTDQIN--LPTVDESSSGLSTCFQQPSDGSTVQVPE-- 396

Query: 367 GGISFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYD 426
             IS +FD GV + +G  + +L    +GV C+ +G S +LGI  +I G + QQ   V YD
Sbjct: 397 --ISMQFDGGV-LNLGE-QNILISPAEGVICLAMGSSSQLGI--SIFGNIQQQETQVLYD 435

BLAST of CsGy3G017770 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.6e-31
Identity = 99/363 (27.27%), Postives = 162/363 (44.63%), Query Frame = 0

Query: 72  IGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTTSFDPSLSSSFSLLPCNHPI 131
           +GTP +   LVLDTGS ++WIQC      +      +     F+P+ SS++  L C+ P 
Sbjct: 168 VGTPAKEMYLVLDTGSDVNWIQC------EPCADCYQQSDPVFNPTSSSTYKSLTCSAPQ 227

Query: 132 CKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLSTPPVILGCAQAS 191
           C        L TS  ++  C Y   Y DG+   G L  +  TF  S     V LGC   +
Sbjct: 228 CS------LLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDN 287

Query: 192 ----TENRGILGMNRGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNSSKFKYVT 251
               T   G+LG+  G LS  +Q K + FSYC+  R            D+  SS   + +
Sbjct: 288 EGLFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDR------------DSGKSSSLDFNS 347

Query: 252 MLTFPESQSSPNLD----PLAYTLPMKAIKIAGKRLNVPPAAFKPDAGGSGQTMIDSGSD 311
           +       ++P L        Y + +    + G+++ +P A F  DA GSG  ++D G+ 
Sbjct: 348 VQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTA 407

Query: 312 LTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEFDNG 371
           +T L  +AY  +++  ++L    +KKG     + D C+D    + V  ++  ++F F  G
Sbjct: 408 VTRLQTQAYNSLRDAFLKLT-VNLKKGSSSISLFDTCYDFSSLSTV--KVPTVAFHFTGG 467

Query: 372 VEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVGFGG 427
             + +     ++   + G  C     +       +IIG V QQ   + YDL+   +G  G
Sbjct: 468 KSLDLPAKNYLIPVDDSGTFCFAFAPTSS---SLSIIGNVQQQGTRITYDLSKNVIGLSG 500

BLAST of CsGy3G017770 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 8.8e-30
Identity = 113/393 (28.75%), Postives = 179/393 (45.55%), Query Frame = 0

Query: 45  YAKRPSSYGSFKLP-FKYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHD-KKIKKR 104
           +A RP  + S  +      S      L +GTP +   +VLDTGS + W+QC   ++   +
Sbjct: 120 HAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQ 179

Query: 105 LPPLPKPKTTSFDPSLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTL 164
             P+       FDP  S +++ +PC+ P C+ R+      T   + + C Y   Y DG+ 
Sbjct: 180 SDPI-------FDPRKSKTYATIPCSSPHCR-RLDSAGCNT---RRKTCLYQVSYGDGSF 239

Query: 165 AEGNLVREKFTFSKSLSTPPVILGCAQAS----TENRGILGMNRGRLSFISQAK---ISK 224
             G+   E  TF ++     V LGC   +        G+LG+ +G+LSF  Q       K
Sbjct: 240 TVGDFSTETLTFRRN-RVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQK 299

Query: 225 FSYCVPSRTGSNPTGLFYLGDNPNSSKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIA 284
           FSYC+  R+ S+       G+   S   ++  +L      S+P LD   Y + +  I + 
Sbjct: 300 FSYCLVDRSASSKPSSVVFGNAAVSRIARFTPLL------SNPKLDTF-YYVGLLGISVG 359

Query: 285 GKRL-NVPPAAFKPDAGGSGQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYA 344
           G R+  V  + FK D  G+G  +IDSG+ +T L+  AY  +++     VGA   K     
Sbjct: 360 GTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAF--RVGAKTLKRAPDF 419

Query: 345 DVADMCFDAGVTAEVGRRIGGISFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLG 404
            + D CFD     EV  ++  +   F  G ++ +     ++     G  C     +  +G
Sbjct: 420 SLFDTCFDLSNMNEV--KVPTVVLHF-RGADVSLPATNYLIPVDTNGKFCFAFAGT--MG 479

Query: 405 IGSNIIGTVHQQNMWVEYDLANKRVGFGGAECS 428
            G +IIG + QQ   V YDLA+ RVGF    C+
Sbjct: 480 -GLSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

BLAST of CsGy3G017770 vs. NCBI nr
Match: XP_004140731.3 (aspartic proteinase PCS1 [Cucumis sativus] >KAE8650484.1 hypothetical protein Csa_011640 [Cucumis sativus])

HSP 1 Score: 852 bits (2200), Expect = 1.67e-311
Identity = 428/430 (99.53%), Postives = 429/430 (99.77%), Query Frame = 0

Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK 60
           MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK
Sbjct: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTTSFDPSLSS 120
           YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKT SFDPSLSS
Sbjct: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTASFDPSLSS 120

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 180
           SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST
Sbjct: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 180

Query: 181 PPVILGCAQASTENRGILGMNRGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNS 240
           PPVILGCAQASTENRGILGMNRGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNS
Sbjct: 181 PPVILGCAQASTENRGILGMNRGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNS 240

Query: 241 SKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLNVPPAAFKPDAGGSGQTMIDS 300
           SKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLN+PPAAFKPDAGGSGQTMIDS
Sbjct: 241 SKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLNIPPAAFKPDAGGSGQTMIDS 300

Query: 301 GSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEF 360
           GSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEF
Sbjct: 301 GSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEF 360

Query: 361 DNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 420
           DNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG
Sbjct: 361 DNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 420

Query: 421 FGGAECSRLK 430
           FGGAECSRLK
Sbjct: 421 FGGAECSRLK 430

BLAST of CsGy3G017770 vs. NCBI nr
Match: XP_011651212.2 (aspartic proteinase PCS1 [Cucumis sativus] >KAE8650483.1 hypothetical protein Csa_010014 [Cucumis sativus])

HSP 1 Score: 811 bits (2095), Expect = 1.74e-295
Identity = 408/431 (94.66%), Postives = 416/431 (96.52%), Query Frame = 0

Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSY-SSQLYAKRPSSYGSFKLPF 60
           MLLILFSLSLFTLSFSQSNSLSLPFPLSL+EKPSN  P Y SSQLY K+PSS+G FKLPF
Sbjct: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLTEKPSNITPLYYSSQLYVKKPSSHGPFKLPF 60

Query: 61  KYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTTSFDPSLS 120
           KYSS+ALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKK+KKRLPPLPKPKT +FDPSLS
Sbjct: 61  KYSSSALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTATFDPSLS 120

Query: 121 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLS 180
           SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFS SLS
Sbjct: 121 SSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLS 180

Query: 181 TPPVILGCAQASTENRGILGMNRGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPN 240
           TPPVILGCAQ STENRGILGMN GRLSFISQAKISKFSYCVPSRTG NPTGLFYLGDNPN
Sbjct: 181 TPPVILGCAQGSTENRGILGMNHGRLSFISQAKISKFSYCVPSRTGPNPTGLFYLGDNPN 240

Query: 241 SSKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLNVPPAAFKPDAGGSGQTMID 300
           SSKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLN+PPAAFKPDAGGSGQTMID
Sbjct: 241 SSKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLNIPPAAFKPDAGGSGQTMID 300

Query: 301 SGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFE 360
           SGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYA VADMCFDAGVT EVGRRIG +SFE
Sbjct: 301 SGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYAAVADMCFDAGVTVEVGRRIGDMSFE 360

Query: 361 FDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRV 420
           FDNGVEIFVGRGEGVLTEVEKGVKCVGIGRS RLGIGSNIIGTVHQQNMWVEYDLANKRV
Sbjct: 361 FDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSGRLGIGSNIIGTVHQQNMWVEYDLANKRV 420

Query: 421 GFGGAECSRLK 430
           GFGGAECSRLK
Sbjct: 421 GFGGAECSRLK 431

BLAST of CsGy3G017770 vs. NCBI nr
Match: XP_008457122.1 (PREDICTED: aspartic proteinase PCS1 [Cucumis melo] >KAA0047379.1 aspartic proteinase PCS1 [Cucumis melo var. makuwa] >TYK14056.1 aspartic proteinase PCS1 [Cucumis melo var. makuwa])

HSP 1 Score: 800 bits (2065), Expect = 6.03e-291
Identity = 401/430 (93.26%), Postives = 413/430 (96.05%), Query Frame = 0

Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK 60
           MLLILFSLSLFTL FSQSNS+SLPFPLSLSEKPSN  P Y SQLYAK+PSS+GSFKLPFK
Sbjct: 1   MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTTSFDPSLSS 120
           YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDK +KK+LPPLPKPKT SFDPSLSS
Sbjct: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDK-VKKKLPPLPKPKTASFDPSLSS 120

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 180
           SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKF+ S SLST
Sbjct: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLST 180

Query: 181 PPVILGCAQASTENRGILGMNRGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNS 240
           PPVILGCAQASTENRGILGMN+GRLSFISQAKISKFSYCVP+RTGSNPTGLFYLGDNPNS
Sbjct: 181 PPVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNS 240

Query: 241 SKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLNVPPAAFKPDAGGSGQTMIDS 300
           S+FKYVTMLTFPESQSSPNLDPLAYTLPMK IKIAGKRLN+ PAAFKPDAGGSGQTMIDS
Sbjct: 241 SRFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDS 300

Query: 301 GSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEF 360
           GSDLTYLVDEAYEKVKEEVVRLVGA MKKGYVYA VADMCFDA VTAEVGRRIGGISFEF
Sbjct: 301 GSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEF 360

Query: 361 DNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 420
           DNGVEI VGRGEGVLTEVEKGVKCVG GRSERLGIGSNIIGTVHQQNMWVEYDL N+R+G
Sbjct: 361 DNGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIG 420

Query: 421 FGGAECSRLK 430
           FGGAECSRLK
Sbjct: 421 FGGAECSRLK 429

BLAST of CsGy3G017770 vs. NCBI nr
Match: XP_038878004.1 (aspartic proteinase PCS1 [Benincasa hispida])

HSP 1 Score: 721 bits (1860), Expect = 8.50e-260
Identity = 372/430 (86.51%), Postives = 389/430 (90.47%), Query Frame = 0

Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK 60
           +L++ FSLSLF+LSFSQSNSLSL FPLSLS+K SN  P Y S LY+K  SS+GSFKLP K
Sbjct: 2   LLILFFSLSLFSLSFSQSNSLSLSFPLSLSKKTSNISPLYPS-LYSKT-SSHGSFKLPVK 61

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTTSFDPSLSS 120
           YS TALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDK  KK    LPKPKT SFDPSLSS
Sbjct: 62  YS-TALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKK----LPKPKTASFDPSLSS 121

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 180
           SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFS SL+T
Sbjct: 122 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLTT 181

Query: 181 PPVILGCAQASTENRGILGMNRGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNS 240
           PPVILGCAQASTENRGILGMN GRLSFISQAKISKFSYCVP RT  NPTGLFYLGDNPNS
Sbjct: 182 PPVILGCAQASTENRGILGMNTGRLSFISQAKISKFSYCVPRRTRFNPTGLFYLGDNPNS 241

Query: 241 SKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLNVPPAAFKPDAGGSGQTMIDS 300
           SKFKYVTMLTFP+SQ SPNLDPLAYTLPMK IKIAGKRLN+ PA FKPDA GSGQTMIDS
Sbjct: 242 SKFKYVTMLTFPKSQRSPNLDPLAYTLPMKGIKIAGKRLNISPAFFKPDASGSGQTMIDS 301

Query: 301 GSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEF 360
           GSDLTYL DEAYEKVKEE+VRLVG MMKKGY +A VADMCF+AG  A VGRRIG +SFEF
Sbjct: 302 GSDLTYLADEAYEKVKEEIVRLVGPMMKKGYEFAAVADMCFNAGEAAVVGRRIGNMSFEF 361

Query: 361 DNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 420
           +NGVEI VG+GEGVL EVEKGVKCVGIGRS RLGIGSNIIGTVHQQNMWVEYDLA KR+G
Sbjct: 362 ENGVEILVGKGEGVLAEVEKGVKCVGIGRSGRLGIGSNIIGTVHQQNMWVEYDLAKKRIG 421

Query: 421 FGGAECSRLK 430
           FGGAECSRLK
Sbjct: 422 FGGAECSRLK 424

BLAST of CsGy3G017770 vs. NCBI nr
Match: XP_022934878.1 (aspartic proteinase PCS1-like [Cucurbita moschata])

HSP 1 Score: 678 bits (1749), Expect = 6.54e-243
Identity = 348/430 (80.93%), Postives = 379/430 (88.14%), Query Frame = 0

Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK 60
           MLL LF LSL +LSFS S+SLSL FPLSLS++PS  + S  S L++   S+YGS KLPFK
Sbjct: 1   MLLSLFYLSLLSLSFSHSHSLSLSFPLSLSKRPS-PVSSSVSPLFSSL-SAYGSVKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTTSFDPSLSS 120
           YS TALVVSLPIGTPPQPTDLVLDTGSQLSWIQCH K  KK L    KPKTTSFDPSLSS
Sbjct: 61  YS-TALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHRKVHKKLL----KPKTTSFDPSLSS 120

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 180
           SFSLLPCNHP+CKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFS S +T
Sbjct: 121 SFSLLPCNHPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSRTT 180

Query: 181 PPVILGCAQASTENRGILGMNRGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNS 240
           PPVILGCAQASTENRGILGMN GRLSF+SQAKISKFSYCVP RTG + TGLFYLGDNPNS
Sbjct: 181 PPVILGCAQASTENRGILGMNTGRLSFVSQAKISKFSYCVPGRTGPDSTGLFYLGDNPNS 240

Query: 241 SKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLNVPPAAFKPDAGGSGQTMIDS 300
           + FKY+++LTFP+SQ SPNLDPLAYTLP+K IKIAG RLN+  A FKPD  GSGQTMIDS
Sbjct: 241 ANFKYISLLTFPKSQRSPNLDPLAYTLPLKGIKIAGNRLNISSAVFKPDRSGSGQTMIDS 300

Query: 301 GSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEF 360
           GSDLTYLVDEAYEKVKEE+V+LVG +MKKGY YA VADMCF+ G TAEVGRRI  +SFEF
Sbjct: 301 GSDLTYLVDEAYEKVKEEIVKLVGPLMKKGYEYAAVADMCFNDGETAEVGRRIRDMSFEF 360

Query: 361 DNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 420
           +NGVEI VG+GEGVLTEVEKGVKCVG GRS RLGI SNIIGTVHQ+N W+EYDLAN+R+G
Sbjct: 361 ENGVEISVGKGEGVLTEVEKGVKCVGFGRSGRLGIASNIIGTVHQKNTWIEYDLANRRIG 420

Query: 421 FGGAECSRLK 430
           FGGA+CSRLK
Sbjct: 421 FGGADCSRLK 423

BLAST of CsGy3G017770 vs. ExPASy TrEMBL
Match: A0A5A7U1M2 (Aspartic proteinase PCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold363G00330 PE=3 SV=1)

HSP 1 Score: 800 bits (2065), Expect = 2.92e-291
Identity = 401/430 (93.26%), Postives = 413/430 (96.05%), Query Frame = 0

Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK 60
           MLLILFSLSLFTL FSQSNS+SLPFPLSLSEKPSN  P Y SQLYAK+PSS+GSFKLPFK
Sbjct: 1   MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTTSFDPSLSS 120
           YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDK +KK+LPPLPKPKT SFDPSLSS
Sbjct: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDK-VKKKLPPLPKPKTASFDPSLSS 120

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 180
           SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKF+ S SLST
Sbjct: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLST 180

Query: 181 PPVILGCAQASTENRGILGMNRGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNS 240
           PPVILGCAQASTENRGILGMN+GRLSFISQAKISKFSYCVP+RTGSNPTGLFYLGDNPNS
Sbjct: 181 PPVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNS 240

Query: 241 SKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLNVPPAAFKPDAGGSGQTMIDS 300
           S+FKYVTMLTFPESQSSPNLDPLAYTLPMK IKIAGKRLN+ PAAFKPDAGGSGQTMIDS
Sbjct: 241 SRFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDS 300

Query: 301 GSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEF 360
           GSDLTYLVDEAYEKVKEEVVRLVGA MKKGYVYA VADMCFDA VTAEVGRRIGGISFEF
Sbjct: 301 GSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEF 360

Query: 361 DNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 420
           DNGVEI VGRGEGVLTEVEKGVKCVG GRSERLGIGSNIIGTVHQQNMWVEYDL N+R+G
Sbjct: 361 DNGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIG 420

Query: 421 FGGAECSRLK 430
           FGGAECSRLK
Sbjct: 421 FGGAECSRLK 429

BLAST of CsGy3G017770 vs. ExPASy TrEMBL
Match: A0A1S3C4D2 (aspartic proteinase PCS1 OS=Cucumis melo OX=3656 GN=LOC103496869 PE=3 SV=1)

HSP 1 Score: 800 bits (2065), Expect = 2.92e-291
Identity = 401/430 (93.26%), Postives = 413/430 (96.05%), Query Frame = 0

Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK 60
           MLLILFSLSLFTL FSQSNS+SLPFPLSLSEKPSN  P Y SQLYAK+PSS+GSFKLPFK
Sbjct: 1   MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTTSFDPSLSS 120
           YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDK +KK+LPPLPKPKT SFDPSLSS
Sbjct: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDK-VKKKLPPLPKPKTASFDPSLSS 120

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 180
           SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKF+ S SLST
Sbjct: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLST 180

Query: 181 PPVILGCAQASTENRGILGMNRGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNS 240
           PPVILGCAQASTENRGILGMN+GRLSFISQAKISKFSYCVP+RTGSNPTGLFYLGDNPNS
Sbjct: 181 PPVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNS 240

Query: 241 SKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLNVPPAAFKPDAGGSGQTMIDS 300
           S+FKYVTMLTFPESQSSPNLDPLAYTLPMK IKIAGKRLN+ PAAFKPDAGGSGQTMIDS
Sbjct: 241 SRFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDS 300

Query: 301 GSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEF 360
           GSDLTYLVDEAYEKVKEEVVRLVGA MKKGYVYA VADMCFDA VTAEVGRRIGGISFEF
Sbjct: 301 GSDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDARVTAEVGRRIGGISFEF 360

Query: 361 DNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 420
           DNGVEI VGRGEGVLTEVEKGVKCVG GRSERLGIGSNIIGTVHQQNMWVEYDL N+R+G
Sbjct: 361 DNGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIG 420

Query: 421 FGGAECSRLK 430
           FGGAECSRLK
Sbjct: 421 FGGAECSRLK 429

BLAST of CsGy3G017770 vs. ExPASy TrEMBL
Match: A0A6J1F8Z2 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111441919 PE=3 SV=1)

HSP 1 Score: 678 bits (1749), Expect = 3.17e-243
Identity = 348/430 (80.93%), Postives = 379/430 (88.14%), Query Frame = 0

Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK 60
           MLL LF LSL +LSFS S+SLSL FPLSLS++PS  + S  S L++   S+YGS KLPFK
Sbjct: 1   MLLSLFYLSLLSLSFSHSHSLSLSFPLSLSKRPS-PVSSSVSPLFSSL-SAYGSVKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTTSFDPSLSS 120
           YS TALVVSLPIGTPPQPTDLVLDTGSQLSWIQCH K  KK L    KPKTTSFDPSLSS
Sbjct: 61  YS-TALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHRKVHKKLL----KPKTTSFDPSLSS 120

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 180
           SFSLLPCNHP+CKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFS S +T
Sbjct: 121 SFSLLPCNHPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSRTT 180

Query: 181 PPVILGCAQASTENRGILGMNRGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNS 240
           PPVILGCAQASTENRGILGMN GRLSF+SQAKISKFSYCVP RTG + TGLFYLGDNPNS
Sbjct: 181 PPVILGCAQASTENRGILGMNTGRLSFVSQAKISKFSYCVPGRTGPDSTGLFYLGDNPNS 240

Query: 241 SKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLNVPPAAFKPDAGGSGQTMIDS 300
           + FKY+++LTFP+SQ SPNLDPLAYTLP+K IKIAG RLN+  A FKPD  GSGQTMIDS
Sbjct: 241 ANFKYISLLTFPKSQRSPNLDPLAYTLPLKGIKIAGNRLNISSAVFKPDRSGSGQTMIDS 300

Query: 301 GSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEF 360
           GSDLTYLVDEAYEKVKEE+V+LVG +MKKGY YA VADMCF+ G TAEVGRRI  +SFEF
Sbjct: 301 GSDLTYLVDEAYEKVKEEIVKLVGPLMKKGYEYAAVADMCFNDGETAEVGRRIRDMSFEF 360

Query: 361 DNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 420
           +NGVEI VG+GEGVLTEVEKGVKCVG GRS RLGI SNIIGTVHQ+N W+EYDLAN+R+G
Sbjct: 361 ENGVEISVGKGEGVLTEVEKGVKCVGFGRSGRLGIASNIIGTVHQKNTWIEYDLANRRIG 420

Query: 421 FGGAECSRLK 430
           FGGA+CSRLK
Sbjct: 421 FGGADCSRLK 423

BLAST of CsGy3G017770 vs. ExPASy TrEMBL
Match: A0A6J1J2U3 (aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111482170 PE=3 SV=1)

HSP 1 Score: 677 bits (1748), Expect = 3.88e-243
Identity = 347/430 (80.70%), Postives = 375/430 (87.21%), Query Frame = 0

Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK 60
           MLL LF +SL +LSFS S+SL L FPLSLS++PS   P +SS       SSYGS KLPFK
Sbjct: 1   MLLSLFYISLLSLSFSHSHSLPLSFPLSLSKRPSPVSPLFSSL------SSYGSVKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTTSFDPSLSS 120
           YS TALVVSLPIGTPPQPTDLVLDTGSQLSWIQCH K  KK L    KPKT SFDPSLSS
Sbjct: 61  YS-TALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHRKLHKKLL----KPKTASFDPSLSS 120

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 180
           SFSLLPCNHP+CKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFS S +T
Sbjct: 121 SFSLLPCNHPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSRTT 180

Query: 181 PPVILGCAQASTENRGILGMNRGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNS 240
           PPVILGCAQASTENRGILGMN GRLSF+SQAKISKFSYCVP RTG + TGLFYLGDNPNS
Sbjct: 181 PPVILGCAQASTENRGILGMNTGRLSFVSQAKISKFSYCVPGRTGPDSTGLFYLGDNPNS 240

Query: 241 SKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLNVPPAAFKPDAGGSGQTMIDS 300
           + FKY+++LTFP+SQ SPNLDPLAYTLP+K IKIAG RLN+  A FKPD  GSGQTMIDS
Sbjct: 241 ANFKYISLLTFPKSQRSPNLDPLAYTLPLKGIKIAGNRLNISSAVFKPDRSGSGQTMIDS 300

Query: 301 GSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEF 360
           GSDLTYLVDEAYEKVKEE+V+LVG +MKKGY YA VADMCF+ G TAEVGRRI  +SFEF
Sbjct: 301 GSDLTYLVDEAYEKVKEEIVKLVGPLMKKGYEYAAVADMCFNDGETAEVGRRIRDMSFEF 360

Query: 361 DNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 420
           +NGVEI VG+GEGVLTEVEKGVKCVG GRS RLGI SNIIGTVHQ+N WVEYDLAN+R+G
Sbjct: 361 ENGVEISVGKGEGVLTEVEKGVKCVGFGRSGRLGIASNIIGTVHQKNTWVEYDLANRRIG 419

Query: 421 FGGAECSRLK 430
           FGGA+CSRLK
Sbjct: 421 FGGADCSRLK 419

BLAST of CsGy3G017770 vs. ExPASy TrEMBL
Match: A0A0A0L6V5 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G188350 PE=3 SV=1)

HSP 1 Score: 637 bits (1643), Expect = 2.15e-228
Identity = 340/430 (79.07%), Postives = 341/430 (79.30%), Query Frame = 0

Query: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK 60
           MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK
Sbjct: 1   MLLILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFK 60

Query: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTTSFDPSLSS 120
           YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKK+KKRLPPLPKPKT SFDPSLSS
Sbjct: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKVKKRLPPLPKPKTASFDPSLSS 120

Query: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 180
           SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST
Sbjct: 121 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 180

Query: 181 PPVILGCAQASTENRGILGMNRGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDNPNS 240
           PPVILGCAQASTENR                                             
Sbjct: 181 PPVILGCAQASTENR--------------------------------------------- 240

Query: 241 SKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLNVPPAAFKPDAGGSGQTMIDS 300
                                                      AAFKPDAGGSGQTMIDS
Sbjct: 241 -------------------------------------------AAFKPDAGGSGQTMIDS 300

Query: 301 GSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEF 360
           GSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEF
Sbjct: 301 GSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEF 342

Query: 361 DNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 420
           DNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG
Sbjct: 361 DNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVG 342

Query: 421 FGGAECSRLK 430
           FGGAECSRLK
Sbjct: 421 FGGAECSRLK 342

BLAST of CsGy3G017770 vs. TAIR 10
Match: AT5G37540.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 554.3 bits (1427), Expect = 8.7e-158
Identity = 284/436 (65.14%), Postives = 338/436 (77.52%), Query Frame = 0

Query: 2   LLILFSLSLFTLSFSQSNSLSLPFPL-SLSEKPSNTIPSYSSQLYAKR----PSSYGSFK 61
           LL +F    +++S S S+SLSL FPL SL   P+    S+ + L ++R    PSS  +F+
Sbjct: 12  LLYIFFFFCYSVSLSWSSSLSLHFPLTSLRLTPTTNSSSFKTSLLSRRNPSPPSSPYTFR 71

Query: 62  LPFKYSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTTSFDP 121
              KY S AL++SLPIGTP Q  +LVLDTGSQLSWIQCH KKIKK LP    P TTSFDP
Sbjct: 72  SNIKY-SMALILSLPIGTPSQSQELVLDTGSQLSWIQCHPKKIKKPLP----PPTTSFDP 131

Query: 122 SLSSSFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSK 181
           SLSSSFS LPC+HP+CKPRIPDFTLPTSCD NRLCHYSYFYADGT AEGNLV+EKFTFS 
Sbjct: 132 SLSSSFSDLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKFTFSN 191

Query: 182 SLSTPPVILGCAQASTENRGILGMNRGRLSFISQAKISKFSYCVPSRT---GSNPTGLFY 241
           S +TPP+ILGCA+ ST+ +GILGMN GRLSFISQAKISKFSYC+P+R+   G   TG FY
Sbjct: 192 SQTTPPLILGCAKESTDEKGILGMNLGRLSFISQAKISKFSYCIPTRSNRPGLASTGSFY 251

Query: 242 LGDNPNSSKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLNVPPAAFKPDAGGS 301
           LGDNPNS  FKYV++LTFP+SQ  PNLDPLAYT+P++ I+I  KRLN+P + F+PDAGGS
Sbjct: 252 LGDNPNSRGFKYVSLLTFPQSQRMPNLDPLAYTVPLQGIRIGQKRLNIPGSVFRPDAGGS 311

Query: 302 GQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRI 361
           GQTM+DSGS+ T+LVD AY+KVKEE+VRLVG+ +KKGYVY   ADMCFD   + E+GR I
Sbjct: 312 GQTMVDSGSEFTHLVDVAYDKVKEEIVRLVGSRLKKGYVYGSTADMCFDGNHSMEIGRLI 371

Query: 362 GGISFEFDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYD 421
           G + FEF  GVEI V + + +L  V  G+ CVGIGRS  LG  SNIIG VHQQN+WVE+D
Sbjct: 372 GDLVFEFGRGVEILVEK-QSLLVNVGGGIHCVGIGRSSMLGAASNIIGNVHQQNLWVEFD 431

Query: 422 LANKRVGFGGAECSRL 430
           + N+RVGF  AEC  L
Sbjct: 432 VTNRRVGFSKAECRLL 441

BLAST of CsGy3G017770 vs. TAIR 10
Match: AT1G66180.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 514.2 bits (1323), Expect = 9.9e-146
Identity = 269/432 (62.27%), Postives = 318/432 (73.61%), Query Frame = 0

Query: 4   ILFSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYG---SFKLPFK 63
           + F   L  +S S S SL LP         +N+    +S L  K PS      +F+  FK
Sbjct: 8   LFFFFFLNYVSLSTSLSLHLPLTSLPISTTTNSHRFTTSLLSRKNPSPSSPPYNFRSRFK 67

Query: 64  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTTSFDPSLSS 123
           Y S AL++SLPIGTPPQ   +VLDTGSQLSWIQCH    +K+LPP PK   TSFDPSLSS
Sbjct: 68  Y-SMALIISLPIGTPPQAQQMVLDTGSQLSWIQCH----RKKLPPKPK---TSFDPSLSS 127

Query: 124 SFSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLST 183
           SFS LPC+HP+CKPRIPDFTLPTSCD NRLCHYSYFYADGT AEGNLV+EK TFS +  T
Sbjct: 128 SFSTLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKITFSNTEIT 187

Query: 184 PPVILGCAQASTENRGILGMNRGRLSFISQAKISKFSYCVP---SRTGSNPTGLFYLGDN 243
           PP+ILGCA  S+++RGILGMNRGRLSF+SQAKISKFSYC+P   +R G  PTG FYLGDN
Sbjct: 188 PPLILGCATESSDDRGILGMNRGRLSFVSQAKISKFSYCIPPKSNRPGFTPTGSFYLGDN 247

Query: 244 PNSSKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLNVPPAAFKPDAGGSGQTM 303
           PNS  FKYV++LTFPESQ  PNLDPLAYT+PM  I+   K+LN+  + F+PDAGGSGQTM
Sbjct: 248 PNSHGFKYVSLLTFPESQRMPNLDPLAYTVPMIGIRFGLKKLNISGSVFRPDAGGSGQTM 307

Query: 304 IDSGSDLTYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGIS 363
           +DSGS+ T+LVD AY+KV+ E++  VG  +KKGYVY   ADMCFD  V A + R IG + 
Sbjct: 308 VDSGSEFTHLVDAAYDKVRAEIMTRVGRRLKKGYVYGGTADMCFDGNV-AMIPRLIGDLV 367

Query: 364 FEFDNGVEIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANK 423
           F F  GVEI V + E VL  V  G+ CVGIGRS  LG  SNIIG VHQQN+WVE+D+ N+
Sbjct: 368 FVFTRGVEILVPK-ERVLVNVGGGIHCVGIGRSSMLGAASNIIGNVHQQNLWVEFDVTNR 427

Query: 424 RVGFGGAECSRL 430
           RVGF  A+CSR+
Sbjct: 428 RVGFAKADCSRV 429

BLAST of CsGy3G017770 vs. TAIR 10
Match: AT5G02190.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 242.3 bits (617), Expect = 7.3e-64
Identity = 159/446 (35.65%), Postives = 232/446 (52.02%), Query Frame = 0

Query: 6   FSLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFKYSSTA 65
           FS S F+   S S+S +L  PL     P++            RP+     KL F ++ T 
Sbjct: 32  FSFSSFS---SSSSSQTLVLPLKTRITPTD-----------HRPTD----KLHFHHNVT- 91

Query: 66  LVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTTSFDPSLSSSFSLL 125
           L V+L +GTPPQ   +V+DTGS+LSW++C+           P P   +FDP+ SSS+S +
Sbjct: 92  LTVTLTVGTPPQNISMVIDTGSELSWLRCNRSS-------NPNP-VNNFDPTRSSSYSPI 151

Query: 126 PCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSKSLSTPPVIL 185
           PC+ P C+ R  DF +P SCD ++LCH +  YAD + +EGNL  E F F  S +   +I 
Sbjct: 152 PCSSPTCRTRTRDFLIPASCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTNDSNLIF 211

Query: 186 GC--------AQASTENRGILGMNRGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDN 245
           GC         +  T+  G+LGMNRG LSFISQ    KFSYC+ S T   P G   LGD 
Sbjct: 212 GCMGSVSGSDPEEDTKTTGLLGMNRGSLSFISQMGFPKFSYCI-SGTDDFP-GFLLLGD- 271

Query: 246 PNSSKFKYVTMLTFPE----SQSSPNLDPLAYTLPMKAIKIAGKRLNVPPAAFKPDAGGS 305
              S F ++T L +      S   P  D +AYT+ +  IK+ GK L +P +   PD  G+
Sbjct: 272 ---SNFTWLTPLNYTPLIRISTPLPYFDRVAYTVQLTGIKVNGKLLPIPKSVLVPDHTGA 331

Query: 306 GQTMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMM----KKGYVYADVADMCF---DAGVT 365
           GQTM+DSG+  T+L+   Y  ++   +     ++       +V+    D+C+      + 
Sbjct: 332 GQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVYEDPDFVFQGTMDLCYRISPVRIR 391

Query: 366 AEVGRRIGGISFEFDNGVEIFVGRGEGVLTEV------EKGVKCVGIGRSERLGIGSNII 425
           + +  R+  +S  F+ G EI V  G+ +L  V         V C   G S+ +G+ + +I
Sbjct: 392 SGILHRLPTVSLVFE-GAEIAVS-GQPLLYRVPHLTVGNDSVYCFTFGNSDLMGMEAYVI 442

Query: 426 GTVHQQNMWVEYDLANKRVGFGGAEC 427
           G  HQQNMW+E+DL   R+G    EC
Sbjct: 452 GHHHQQNMWIEFDLQRSRIGLAPVEC 442

BLAST of CsGy3G017770 vs. TAIR 10
Match: AT2G39710.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 241.1 bits (614), Expect = 1.6e-63
Identity = 158/439 (35.99%), Postives = 241/439 (54.90%), Query Frame = 0

Query: 7   SLSLFTLSFSQSNSLSLPFPLSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFKYSSTAL 66
           SLSL + +F + + L L FPL+   K S+T  +    L  ++     S KL F+++ T L
Sbjct: 9   SLSL-SKNFLRISVLLLIFPLTFC-KTSSTNQTLLFSLKTQKLPQSSSDKLSFRHNVT-L 68

Query: 67  VVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKKIKKRLPPLPKPKTTSFDPSLSSSFSLLP 126
            V+L +G PPQ   +VLDTGS+LSW+ C      K+ P L     + F+P  SS++S +P
Sbjct: 69  TVTLAVGDPPQNISMVLDTGSELSWLHC------KKSPNL----GSVFNPVSSSTYSPVP 128

Query: 127 CNHPICKPRIPDFTLPTSCD-QNRLCHYSYFYADGTLAEGNLVREKFTFSKSLSTPPVIL 186
           C+ PIC+ R  D  +P SCD +  LCH +  YAD T  EGNL  E F    S++ P  + 
Sbjct: 129 CSSPICRTRTRDLPIPASCDPKTHLCHVAISYADATSIEGNLAHETFVIG-SVTRPGTLF 188

Query: 187 GC--------AQASTENRGILGMNRGRLSFISQAKISKFSYCVPSRTGSNPTGLFYLGDN 246
           GC        ++   ++ G++GMNRG LSF++Q   SKFSYC+   +GS+ +G   LGD 
Sbjct: 189 GCMDSGLSSNSEEDAKSTGLMGMNRGSLSFVNQLGFSKFSYCI---SGSDSSGFLLLGDA 248

Query: 247 PNS--SKFKYVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLNVPPAAFKPDAGGSGQ 306
             S     +Y  ++   +S   P  D +AYT+ ++ I++  K L++P + F PD  G+GQ
Sbjct: 249 SYSWLGPIQYTPLVL--QSTPLPYFDRVAYTVQLEGIRVGSKILSLPKSVFVPDHTGAGQ 308

Query: 307 TMIDSGSDLTYLVDEAYEKVKEEVVRLVGAMMK----KGYVYADVADMCFDAGVTAEVG- 366
           TM+DSG+  T+L+   Y  +K E +    ++++      +V+    D+C+  G T     
Sbjct: 309 TMVDSGTQFTFLMGPVYTALKNEFITQTKSVLRLVDDPDFVFQGTMDLCYKVGSTTRPNF 368

Query: 367 RRIGGISFEFDNGVEIFVG------RGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVH 424
             +  +S  F  G E+ V       R  G  +E ++ V C   G S+ LGI + +IG  H
Sbjct: 369 SGLPMVSLMF-RGAEMSVSGQKLLYRVNGAGSEGKEEVYCFTFGNSDLLGIEAFVIGHHH 427

BLAST of CsGy3G017770 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 154.8 bits (390), Expect = 1.5e-37
Identity = 125/423 (29.55%), Postives = 188/423 (44.44%), Query Frame = 0

Query: 27  LSLSEKPSNTIPSYSSQLYAKRPSSYGSFKLPFKYSSTALVVSLPIGTPPQPTDLVLDTG 86
           LSL  KP   IP   S + +   S  G +            V L IG PPQ   L+ DTG
Sbjct: 59  LSLRRKP---IPFVKSPVVSGAASGSGQY-----------FVDLRIGQPPQSLLLIADTG 118

Query: 87  SQLSWIQCHDKKIKKRLPPLPKPKTTSFDPSLSSSFSLLPCNHPICKPRIPDFTLPTSCD 146
           S L W++C   +      P      T F P  SS+FS   C  P+C+  +P       C+
Sbjct: 119 SDLVWVKCSACRNCSHHSP-----ATVFFPRHSSTFSPAHCYDPVCR-LVPKPDRAPICN 178

Query: 147 QNRL---CHYSYFYADGTLAEGNLVRE----KFTFSKSLSTPPVILGC----------AQ 206
             R+   CHY Y YADG+L  G   RE    K +  K      V  GC            
Sbjct: 179 HTRIHSTCHYEYGYADGSLTSGLFARETTSLKTSSGKEARLKSVAFGCGFRISGQSVSGT 238

Query: 207 ASTENRGILGMNRGRLSFISQAKI---SKFSYCVPSRTGS-NPTGLFYLGDNPNS-SKFK 266
           +     G++G+ RG +SF SQ      +KFSYC+   T S  PT    +G+  +  SK  
Sbjct: 239 SFNGANGVMGLGRGPISFASQLGRRFGNKFSYCLMDYTLSPPPTSYLIIGNGGDGISKLF 298

Query: 267 YVTMLTFPESQSSPNLDPLAYTLPMKAIKIAGKRLNVPPAAFKPDAGGSGQTMIDSGSDL 326
           +  +LT P       L P  Y + +K++ + G +L + P+ ++ D  G+G T++DSG+ L
Sbjct: 299 FTPLLTNP-------LSPTFYYVKLKSVFVNGAKLRIDPSIWEIDDSGNGGTVVDSGTTL 358

Query: 327 TYLVDEAYEKVKEEVVRLVGAMMKKGYVYADVADMCFDAGVTAEVGRRIGGISFEFDNGV 386
            +L + AY  V   V R V   +          D+C +     +  + +  + FEF  G 
Sbjct: 359 AFLAEPAYRSVIAAVRRRVKLPIADALTPG--FDLCVNVSGVTKPEKILPRLKFEFSGGA 418

Query: 387 EIFVGRGEGVLTEVEKGVKCVGIGRSERLGIGSNIIGTVHQQNMWVEYDLANKRVGFGGA 428
            +FV        E E+ ++C+ I +S    +G ++IG + QQ    E+D    R+GF   
Sbjct: 419 -VFVPPPRNYFIETEEQIQCLAI-QSVDPKVGFSVIGNLMQQGFLFEFDRDRSRLGFSRR 450

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LZL31.0e-6235.65Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1[more]
Q766C33.8e-4131.88Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q766C22.3e-3831.90Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q9LS401.6e-3127.27Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LNJ38.8e-3028.75Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Match NameE-valueIdentityDescription
XP_004140731.31.67e-31199.53aspartic proteinase PCS1 [Cucumis sativus] >KAE8650484.1 hypothetical protein Cs... [more]
XP_011651212.21.74e-29594.66aspartic proteinase PCS1 [Cucumis sativus] >KAE8650483.1 hypothetical protein Cs... [more]
XP_008457122.16.03e-29193.26PREDICTED: aspartic proteinase PCS1 [Cucumis melo] >KAA0047379.1 aspartic protei... [more]
XP_038878004.18.50e-26086.51aspartic proteinase PCS1 [Benincasa hispida][more]
XP_022934878.16.54e-24380.93aspartic proteinase PCS1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A5A7U1M22.92e-29193.26Aspartic proteinase PCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3C4D22.92e-29193.26aspartic proteinase PCS1 OS=Cucumis melo OX=3656 GN=LOC103496869 PE=3 SV=1[more]
A0A6J1F8Z23.17e-24380.93aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111441919 PE=3... [more]
A0A6J1J2U33.88e-24380.70aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111482170 PE=3 S... [more]
A0A0A0L6V52.15e-22879.07Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G18835... [more]
Match NameE-valueIdentityDescription
AT5G37540.18.7e-15865.14Eukaryotic aspartyl protease family protein [more]
AT1G66180.19.9e-14662.27Eukaryotic aspartyl protease family protein [more]
AT5G02190.17.3e-6435.65Eukaryotic aspartyl protease family protein [more]
AT2G39710.11.6e-6335.99Eukaryotic aspartyl protease family protein [more]
AT3G25700.11.5e-3729.55Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 398..413
score: 25.04
coord: 296..307
score: 28.66
coord: 72..92
score: 47.5
IPR001461Aspartic peptidase A1 familyPANTHERPTHR47965ASPARTYL PROTEASE-RELATEDcoord: 2..429
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 235..430
e-value: 2.2E-38
score: 133.7
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 43..234
e-value: 4.4E-37
score: 129.9
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 63..428
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 68..236
e-value: 1.9E-36
score: 126.0
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 264..421
e-value: 7.1E-32
score: 110.4
NoneNo IPR availablePANTHERPTHR47965:SF51EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEINcoord: 2..429
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 81..92
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 66..422
score: 32.095337
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 67..426
e-value: 2.71044E-74
score: 231.383

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy3G017770.1CsGy3G017770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity