CsGy3G008220 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy3G008220
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionEpimerase domain-containing protein
LocationGy14Chr3: 6884163 .. 6885840 (-)
RNA-Seq ExpressionCsGy3G008220
SyntenyCsGy3G008220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TATAAATACGAGAAAATAATATATGTAAGGTCGAGAAAACAATTCATTATAGTATATATAAAGAAGAAAGAGATTTTGGGAATTATGAACAAGTTTACAAAGTGAATTAAAGATCAGAGGATAAGAATTTGAAGAGAAGGGCAAAGAAAATTAACTAAAATGGCAGTGTCGTCTGATTGCTTGAGAGTGTGCGTTACAGGTGGCTCCGGCTACGTCGCATCTTCCCTCATCAAAAAGCTTCTCCTAAATGGCCACATAGTTCATGCAACTCTTCGCAACTTAGGTAAAGTATTCCCTATCCAAATCTCAATATTTTACGGGTTAAAATGTGTATTTGATCAATCTGTTGTCTATATTTTCACGAGGTGAAAAACTGTTTGTTGTTTGTGGACGTAAATAACACAGTATTGGAAAATTTTTATGTTTAAGGTGATGAATCAAAAGTGGGGATTTTGAAGAGGCTCCCAAATGCAACTAATAATTTAGTGTTGTTTAAAGCCGACATTTATGAACCACATCAGTTTGAAGCTGCCATCCGAGGCTGTCATATCGTTTTTCATGTCGCTACTCCTTTACACCACACTCATGCAACTCAGGTTTGGGTTGATTTCTTTTTCTAATCAAAACTATTTTTGGGAGGTTTTGTCGCTCAATTTATGTATAGTTGAAAAGTTTTGTATATATGCGAGTTTAACAAATCTAAATGTATAGTTGCAATTTTTTTTTGTACATATATTTTAACTTGAAATGAATATACTGTAAAAAAATATAGTACATGGACGTGACTGAAGCATCGGTTGCAGCGGCAAAGAAGATAGCGAAATTGTGTGTGGAATTGGGAACGGTTAGGCGGTTGATCTACACAGCCTCTATCGTGTCGATGTCGCCGATGAAAGAAGACGGCAGTGGTTTTAAGGACTTCCTTGACGAAAGCTGTTGGACGCCTCTCAATCTTTCCTATCCTTTTTCTAACTCATTTTTGGTGGTATCAACCCAACTTTTCATCTCTTACTGTTACTGTGGTCTTTAATCAAATTTATAAACTTTAACTTATAATTTAACTCATCAGGGATACCTAGATTCAAAAACAATTACAGAGAAAGAGTTGCTTAAATTTGGGAAGAGCGAGGAATCGAAAGGGTTGGAAGTGGTTTCGTTGGTGTGCGGCCTCGTTGCTGGGGAGTCTCCTCATCCTTCTGCTGCTATAACCACCATGGTCACATTCTCTCAGTTCATCCACGAAAGTGAACCCTTCAAATTCCTAAGATTTCTTGAGGAGTTGGATGGTAAAGTCCCACTCGTACACATTGATGATGTTTGTGAAGCCCACATTTTCTGTATGGAACAAACTTCAATTCATGGCAGATTCTTGTGTGCATCTTCCTTCTTGTCCTCTACAGAAATTGCCAATTACTACCATCTTCACCATTCTCATTTGCAACAAAAACATGGGTAATATAATTATCGGATAATCATTTACGTCATGTTTATTAAATACTATTACACTAAATTCTTATGTCAAATTTTCTAAAATTGTAGGAAATTGGATGAAGTGGCCAAAAGGAACATCAAAATGAATTCCAAGAAGTTGATTGAGCGAGGTTTCATATATAAGTATGATGGTGATATGATACTTGAAGATGCTTTCCATTGCTGCAAAAACCAATTTGTTGGTTAA

mRNA sequence

TATAAATACGAGAAAATAATATATGTAAGGTCGAGAAAACAATTCATTATAGTATATATAAAGAAGAAAGAGATTTTGGGAATTATGAACAAGTTTACAAAGTGAATTAAAGATCAGAGGATAAGAATTTGAAGAGAAGGGCAAAGAAAATTAACTAAAATGGCAGTGTCGTCTGATTGCTTGAGAGTGTGCGTTACAGGTGGCTCCGGCTACGTCGCATCTTCCCTCATCAAAAAGCTTCTCCTAAATGGCCACATAGTTCATGCAACTCTTCGCAACTTAGGTGATGAATCAAAAGTGGGGATTTTGAAGAGGCTCCCAAATGCAACTAATAATTTAGTGTTGTTTAAAGCCGACATTTATGAACCACATCAGTTTGAAGCTGCCATCCGAGGCTGTCATATCGTTTTTCATGTCGCTACTCCTTTACACCACACTCATGCAACTCAGTACATGGACGTGACTGAAGCATCGGTTGCAGCGGCAAAGAAGATAGCGAAATTGTGTGTGGAATTGGGAACGGTTAGGCGGTTGATCTACACAGCCTCTATCGTGTCGATGTCGCCGATGAAAGAAGACGGCAGTGGTTTTAAGGACTTCCTTGACGAAAGCTGTTGGACGCCTCTCAATCTTTCCTATCCTTTTTCTAACTCATTTTTGGTGGGATACCTAGATTCAAAAACAATTACAGAGAAAGAGTTGCTTAAATTTGGGAAGAGCGAGGAATCGAAAGGGTTGGAAGTGGTTTCGTTGGTGTGCGGCCTCGTTGCTGGGGAGTCTCCTCATCCTTCTGCTGCTATAACCACCATGGTCACATTCTCTCAGTTCATCCACGAAAGTGAACCCTTCAAATTCCTAAGATTTCTTGAGGAGTTGGATGGTAAAGTCCCACTCGTACACATTGATGATGTTTGTGAAGCCCACATTTTCTGTATGGAACAAACTTCAATTCATGGCAGATTCTTGTGTGCATCTTCCTTCTTGTCCTCTACAGAAATTGCCAATTACTACCATCTTCACCATTCTCATTTGCAACAAAAACATGGGAAATTGGATGAAGTGGCCAAAAGGAACATCAAAATGAATTCCAAGAAGTTGATTGAGCGAGGTTTCATATATAAGTATGATGGTGATATGATACTTGAAGATGCTTTCCATTGCTGCAAAAACCAATTTGTTGGTTAA

Coding sequence (CDS)

ATGGCAGTGTCGTCTGATTGCTTGAGAGTGTGCGTTACAGGTGGCTCCGGCTACGTCGCATCTTCCCTCATCAAAAAGCTTCTCCTAAATGGCCACATAGTTCATGCAACTCTTCGCAACTTAGGTGATGAATCAAAAGTGGGGATTTTGAAGAGGCTCCCAAATGCAACTAATAATTTAGTGTTGTTTAAAGCCGACATTTATGAACCACATCAGTTTGAAGCTGCCATCCGAGGCTGTCATATCGTTTTTCATGTCGCTACTCCTTTACACCACACTCATGCAACTCAGTACATGGACGTGACTGAAGCATCGGTTGCAGCGGCAAAGAAGATAGCGAAATTGTGTGTGGAATTGGGAACGGTTAGGCGGTTGATCTACACAGCCTCTATCGTGTCGATGTCGCCGATGAAAGAAGACGGCAGTGGTTTTAAGGACTTCCTTGACGAAAGCTGTTGGACGCCTCTCAATCTTTCCTATCCTTTTTCTAACTCATTTTTGGTGGGATACCTAGATTCAAAAACAATTACAGAGAAAGAGTTGCTTAAATTTGGGAAGAGCGAGGAATCGAAAGGGTTGGAAGTGGTTTCGTTGGTGTGCGGCCTCGTTGCTGGGGAGTCTCCTCATCCTTCTGCTGCTATAACCACCATGGTCACATTCTCTCAGTTCATCCACGAAAGTGAACCCTTCAAATTCCTAAGATTTCTTGAGGAGTTGGATGGTAAAGTCCCACTCGTACACATTGATGATGTTTGTGAAGCCCACATTTTCTGTATGGAACAAACTTCAATTCATGGCAGATTCTTGTGTGCATCTTCCTTCTTGTCCTCTACAGAAATTGCCAATTACTACCATCTTCACCATTCTCATTTGCAACAAAAACATGGGAAATTGGATGAAGTGGCCAAAAGGAACATCAAAATGAATTCCAAGAAGTTGATTGAGCGAGGTTTCATATATAAGTATGATGGTGATATGATACTTGAAGATGCTTTCCATTGCTGCAAAAACCAATTTGTTGGTTAA

Protein sequence

MAVSSDCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNLVLFKADIYEPHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELGTVRRLIYTASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITEKELLKFGKSEESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEELDGKVPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHGKLDEVAKRNIKMNSKKLIERGFIYKYDGDMILEDAFHCCKNQFVG*
Homology
BLAST of CsGy3G008220 vs. ExPASy Swiss-Prot
Match: Q5XLY0 (Putative anthocyanidin reductase OS=Ginkgo biloba OX=3311 PE=2 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.4e-43
Identity = 108/336 (32.14%), Postives = 180/336 (53.57%), Query Frame = 0

Query: 10  VCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNLVLFKADIYE 69
           VCVTG +G++AS L+K+LL  G+IVHAT+R+  +++KV  L  LP AT+ L LF+A++ E
Sbjct: 15  VCVTGAAGFMASWLVKRLLEKGYIVHATVRDPENKAKVSHLLNLPGATDRLKLFRAELCE 74

Query: 70  PHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELGTVRRLIYTA 129
              F+AA+ GC+ VFHVATP          D+ + ++     + K C ++ +++R++ T+
Sbjct: 75  DGSFDAAVAGCNGVFHVATPTEFMPKDPENDLIKPAIEGTLNVLKSCTKVDSIKRVVVTS 134

Query: 130 SIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLV-------GYLDSKTITEKELL 189
           S  ++S    + S    ++DESCWT +N        FL         Y  SKT+ E+  L
Sbjct: 135 SAATVS--INNSSEQNQYIDESCWTDVN--------FLTSQKPPGWAYPVSKTLAEQAAL 194

Query: 190 KFGKSEESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEELDGK 249
           K+    E   L+VV+++  LV G +  P+   +  +  S    +      L+ ++ + G 
Sbjct: 195 KYA---EEHSLDVVTVIPVLVVGPAVTPTVPSSVELALSLITGDEFKMGALKGMQFVSGS 254

Query: 250 VPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHGKLDEVA 309
           + LVHIDDVC A IF ME+ S  GR++C        ++A +    +   +      D  A
Sbjct: 255 ISLVHIDDVCSAQIFLMEKPSAQGRYICFPVNTGIPQLAEFLSKRYPQYKVPTKFDDVPA 314

Query: 310 KRNIKMNSKKLIERGFIYKYDGDMILEDAFHCCKNQ 339
              + ++S+KL++ GF +KY  + I + A    K +
Sbjct: 315 TPKLTISSQKLLDCGFSFKYGIEDIYDQAIEYMKTK 337

BLAST of CsGy3G008220 vs. ExPASy Swiss-Prot
Match: Q40316 (Vestitone reductase OS=Medicago sativa OX=3879 PE=1 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 9.7e-40
Identity = 113/333 (33.93%), Postives = 171/333 (51.35%), Query Frame = 0

Query: 9   RVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESK-VGILKRLPNATNNLVLFKADI 68
           RVCVTGG+G++ S +IK LL NG+ V+ T+R   +  + V  L  LP A+  L  F AD+
Sbjct: 7   RVCVTGGTGFLGSWIIKSLLENGYSVNTTIRADPERKRDVSFLTNLPGASEKLHFFNADL 66

Query: 69  YEPHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELGTVRRLIY 128
             P  F AAI GC  +FH A+P+    +     VT+ +V  A  I K CV   TV+R IY
Sbjct: 67  SNPDSFAAAIEGCVGIFHTASPIDFAVSEPEEIVTKRTVDGALGILKACVNSKTVKRFIY 126

Query: 129 TASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITEKELLKFGKS 188
           T+S  ++S   +D    KD LDES W+ ++L       F   Y  SKT+ EK +L+FG  
Sbjct: 127 TSSGSAVSFNGKD----KDVLDESDWSDVDLLRSV-KPFGWNYAVSKTLAEKAVLEFG-- 186

Query: 189 EESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEELDGKVPLVH 248
            E  G++VV+L+   + G    P    +        + + E     RF         +VH
Sbjct: 187 -EQNGIDVVTLILPFIVGRFVCPKLPDSIEKALVLVLGKKEQIGVTRF--------HMVH 246

Query: 249 IDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQ-QKHGKLDEV-AKRN 308
           +DDV  AHI+ +E +   GR+ C+   +   E++      +   Q     +L E+   R 
Sbjct: 247 VDDVARAHIYLLENSVPGGRYNCSPFIVPIEEMSQLLSAKYPEYQILTVDELKEIKGARL 306

Query: 309 IKMNSKKLIERGFIYKYDGDMILEDAFHCCKNQ 339
             +N+KKL++ GF +KY  + + +DA  CCK +
Sbjct: 307 PDLNTKKLVDAGFDFKYTIEDMFDDAIQCCKEK 323

BLAST of CsGy3G008220 vs. ExPASy Swiss-Prot
Match: Q7PCC4 (Anthocyanidin reductase ((2S)-flavan-3-ol-forming) OS=Vitis vinifera OX=29760 GN=ANR PE=1 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.1e-38
Identity = 99/326 (30.37%), Postives = 174/326 (53.37%), Query Frame = 0

Query: 11  CVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNLVLFKADIYEP 70
           CV GG+G+VAS L+K LL  G+ V+ T+R+  ++ KV  L  L     +L +F+AD+ + 
Sbjct: 13  CVVGGTGFVASLLVKLLLQKGYAVNTTVRDPDNQKKVSHLLEL-QELGDLKIFRADLTDE 72

Query: 71  HQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELGTVRRLIYTAS 130
             FEA I GC  VFHVATP+H        D+ + ++     + K C    +V+R+I T+S
Sbjct: 73  LSFEAPIAGCDFVFHVATPVHFASEDPENDMIKPAIQGVVNVMKACTRAKSVKRVILTSS 132

Query: 131 IVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITEKELLKFGKSEES 190
             +++  + DG+G    +DE  WT +      +     GY  SKT+ EK   KF    E 
Sbjct: 133 AAAVTINQLDGTGL--VVDEKNWTDIEF-LTSAKPPTWGYPASKTLAEKAAWKFA---EE 192

Query: 191 KGLEVVSLVCGLVAGES-----PHPSAAITTMVTFSQFIHESEPFKFLRFLEELDGKVPL 250
             +++++++  L+AG S     P       +++T ++F+        ++ ++ L G V +
Sbjct: 193 NNIDLITVIPTLMAGSSLTSDVPSSIGLAMSLITGNEFL-----INGMKGMQMLSGSVSI 252

Query: 251 VHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHGKLDEVAKRN 310
            H++DVC+AHIF  E+ S  GR++C ++  S  E+A +    +   +      D   K  
Sbjct: 253 AHVEDVCQAHIFVAEKESASGRYICCAANTSVPELAKFLSKRYPQYKVPTDFGDFPPKSK 312

Query: 311 IKMNSKKLIERGFIYKYDGDMILEDA 332
           + ++S+KL++ GF +KY  + I +++
Sbjct: 313 LIISSEKLVKEGFSFKYGIEEIYDES 326

BLAST of CsGy3G008220 vs. ExPASy Swiss-Prot
Match: D7U6G6 (Anthocyanidin reductase ((2S)-flavan-3-ol-forming) OS=Vitis vinifera OX=29760 GN=ANR PE=3 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.4e-38
Identity = 100/326 (30.67%), Postives = 173/326 (53.07%), Query Frame = 0

Query: 11  CVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNLVLFKADIYEP 70
           CV GG+G+VAS L+K LL  G+ V+ T+R+  ++ KV  L  L     +L +F+AD+ + 
Sbjct: 13  CVVGGTGFVASLLVKLLLQKGYAVNTTVRDPDNQKKVSHLLEL-QELGDLKIFRADLTDE 72

Query: 71  HQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELGTVRRLIYTAS 130
             FEA I GC  VFHVATP+H        D+ + +V     + K C    +V+R+I T+S
Sbjct: 73  LSFEAPIAGCDFVFHVATPVHFASEDPENDMIKPAVQGVVNVMKACTRAKSVKRVILTSS 132

Query: 131 IVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITEKELLKFGKSEES 190
             +++  + DG+G    +DE  WT +      +     GY  SKT+ EK   KF    E 
Sbjct: 133 AAAVTINQLDGTGL--VVDEKNWTDIEF-LTSAKPPTWGYPASKTLAEKAAWKFA---EE 192

Query: 191 KGLEVVSLVCGLVAGES-----PHPSAAITTMVTFSQFIHESEPFKFLRFLEELDGKVPL 250
             +++++++  L+AG S     P       +++T ++F+        ++ ++ L G V +
Sbjct: 193 NNIDLITVIPTLMAGSSLTSDVPSSIGLAMSLITGNEFL-----INGMKGMQMLSGSVSI 252

Query: 251 VHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHGKLDEVAKRN 310
            H++DVC AHIF  E+ S  GR++C ++  S  E+A +    +   +      D  +K  
Sbjct: 253 AHVEDVCRAHIFVAEKESASGRYICCAANTSVPELAKFLSKRYPQYKVPTDFGDFPSKSK 312

Query: 311 IKMNSKKLIERGFIYKYDGDMILEDA 332
           + ++S KL++ GF +KY  + I +++
Sbjct: 313 LIISSDKLVKEGFSFKYGIEEIYDES 326

BLAST of CsGy3G008220 vs. ExPASy Swiss-Prot
Match: P51103 (Dihydroflavonol 4-reductase OS=Callistephus chinensis OX=13379 GN=F PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.4e-38
Identity = 102/331 (30.82%), Postives = 167/331 (50.45%), Query Frame = 0

Query: 10  VCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNLVLFKADIYE 69
           VCVTG +G++ S L+ +LL  G+IV AT+RN GD  KV  L  LP A  NL L+KAD+ +
Sbjct: 9   VCVTGAAGFIGSWLVMRLLERGYIVRATVRNPGDMKKVKHLLELPKAETNLTLWKADLTQ 68

Query: 70  PHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELGTVRRLIYTA 129
              F+ AI GCH VFHVATP+         ++ + ++     I + C +  TV++L+YT+
Sbjct: 69  EGSFDEAIEGCHGVFHVATPMDFESKDPENEIIKPTIEGILSIIRSCAKAKTVKKLVYTS 128

Query: 130 SIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITEKELLKFGKSEE 189
           S  +++  +          DES W+ L+  Y    +  + Y  SKT+ EK  ++  K   
Sbjct: 129 SAGTVNVQETQ----LPVYDESHWSDLDFIYSKKMTAWM-YFVSKTLAEKAAMEAAK--- 188

Query: 190 SKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEELDGKVPLVHID 249
              ++ VS++  LV G   +P+   + +   S        +  ++       +   VH+D
Sbjct: 189 ENNIDFVSIIPPLVVGPFINPTFPPSLITALSLINGAESHYSIIK-------QGQYVHLD 248

Query: 250 DVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLH--HSHLQQKHGKLDEVAKRNIK 309
           D+CE HIF  E     GR++C+    +  ++A          H+  +   +DE     + 
Sbjct: 249 DLCECHIFLYENPEAKGRYICSKQDATIHQLARMIKQKWPEYHVPTQFAGIDEELP-TVS 308

Query: 310 MNSKKLIERGFIYKYDGDMILEDAFHCCKNQ 339
            +SKKLI+ GF +KYD + + + A   CK +
Sbjct: 309 FSSKKLIDMGFKFKYDLEDMFKGAIDSCKEK 323

BLAST of CsGy3G008220 vs. NCBI nr
Match: XP_004134310.3 (putative anthocyanidin reductase isoform X1 [Cucumis sativus] >KAE8650235.1 hypothetical protein Csa_010630 [Cucumis sativus])

HSP 1 Score: 689 bits (1778), Expect = 3.35e-250
Identity = 340/341 (99.71%), Postives = 341/341 (100.00%), Query Frame = 0

Query: 1   MAVSSDCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNL 60
           MAVSSDCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNL
Sbjct: 1   MAVSSDCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNL 60

Query: 61  VLFKADIYEPHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELG 120
           VLFKADIYEPHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELG
Sbjct: 61  VLFKADIYEPHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELG 120

Query: 121 TVRRLIYTASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITEKE 180
           TVRRLIYTASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITEKE
Sbjct: 121 TVRRLIYTASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITEKE 180

Query: 181 LLKFGKSEESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEELD 240
           LLKFGKSEESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEELD
Sbjct: 181 LLKFGKSEESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEELD 240

Query: 241 GKVPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHGKLDE 300
           GKVPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHGKLDE
Sbjct: 241 GKVPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHGKLDE 300

Query: 301 VAKRNIKMNSKKLIERGFIYKYDGDMILEDAFHCCKNQFVG 341
           VAKRNIKMNSKKLIERGF+YKYDGDMILEDAFHCCKNQFVG
Sbjct: 301 VAKRNIKMNSKKLIERGFVYKYDGDMILEDAFHCCKNQFVG 341

BLAST of CsGy3G008220 vs. NCBI nr
Match: XP_008437918.1 (PREDICTED: vestitone reductase-like [Cucumis melo] >KAA0048905.1 vestitone reductase-like [Cucumis melo var. makuwa])

HSP 1 Score: 622 bits (1603), Expect = 1.70e-223
Identity = 307/343 (89.50%), Postives = 321/343 (93.59%), Query Frame = 0

Query: 1   MAVSSD--CLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATN 60
           MAVSS   CLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNL DESK+GILKRLPNAT 
Sbjct: 1   MAVSSSSGCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLDDESKIGILKRLPNATT 60

Query: 61  NLVLFKADIYEPHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVE 120
           NLVLFKADIYEPH+FEAAIRGCHIVFHVATPLHHTHATQY DVTEASVAAAK IAKLCVE
Sbjct: 61  NLVLFKADIYEPHEFEAAIRGCHIVFHVATPLHHTHATQYSDVTEASVAAAKMIAKLCVE 120

Query: 121 LGTVRRLIYTASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITE 180
           LGTVRRLIYTAS+VSMSPMK+DGSGFKD LDE+CWTPL LSYPFSNS +VGY+DSKT TE
Sbjct: 121 LGTVRRLIYTASVVSMSPMKDDGSGFKDVLDENCWTPLKLSYPFSNSTVVGYVDSKTATE 180

Query: 181 KELLKFGKSEESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEE 240
           KELLKFG+SEES  LEVV+L CGL+AG++ HPS A TT VTFSQFI ESEPFK LRFLEE
Sbjct: 181 KELLKFGESEESMKLEVVTLACGLIAGDTLHPSPAATTTVTFSQFIDESEPFKLLRFLEE 240

Query: 241 LDGKVPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHGKL 300
           LDGKVPLVHIDDVCEAHIFCMEQTSIHGRFLCA+SFLSS EIANYYHLHHSHLQQKHGKL
Sbjct: 241 LDGKVPLVHIDDVCEAHIFCMEQTSIHGRFLCATSFLSSPEIANYYHLHHSHLQQKHGKL 300

Query: 301 DEVAKRNIKMNSKKLIERGFIYKYDGDMILEDAFHCCKNQFVG 341
           DEVAKRNIKMNSKKLIERGFIYKYDGDMILED FHCCKNQF+G
Sbjct: 301 DEVAKRNIKMNSKKLIERGFIYKYDGDMILEDVFHCCKNQFLG 343

BLAST of CsGy3G008220 vs. NCBI nr
Match: TYK17664.1 (vestitone reductase-like [Cucumis melo var. makuwa])

HSP 1 Score: 617 bits (1590), Expect = 1.63e-221
Identity = 305/343 (88.92%), Postives = 319/343 (93.00%), Query Frame = 0

Query: 1   MAVSSD--CLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATN 60
           MAVSS   CLRVCVT GSGYVASS IKKLLLNGHIVHATLRNL DESK+GILKRLPNAT 
Sbjct: 1   MAVSSSSGCLRVCVTCGSGYVASSFIKKLLLNGHIVHATLRNLDDESKIGILKRLPNATT 60

Query: 61  NLVLFKADIYEPHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVE 120
           NLVLFKADIYEPH+FEAAIRGCHIVFHVATPLHHTHATQY DVTEASVAAAK IAKLCVE
Sbjct: 61  NLVLFKADIYEPHEFEAAIRGCHIVFHVATPLHHTHATQYSDVTEASVAAAKMIAKLCVE 120

Query: 121 LGTVRRLIYTASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITE 180
           LGTVRRLIYTAS+VSMSPMK+DGSGFKD LDE+CWTPL LSYPFSNS +VGY+DSKT TE
Sbjct: 121 LGTVRRLIYTASVVSMSPMKDDGSGFKDVLDENCWTPLKLSYPFSNSTVVGYVDSKTATE 180

Query: 181 KELLKFGKSEESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEE 240
           KELLKFG+SEES  LEVV+L CGL+AG++ HPS A TT VTFSQFI ESEPFK LRFLEE
Sbjct: 181 KELLKFGESEESMKLEVVTLACGLIAGDTLHPSPAATTTVTFSQFIDESEPFKLLRFLEE 240

Query: 241 LDGKVPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHGKL 300
           LDGKVPLVHIDDVCEAHIFCMEQTSIHGRFLCA+SFLSS EIANYYHLHHSHLQQKHGKL
Sbjct: 241 LDGKVPLVHIDDVCEAHIFCMEQTSIHGRFLCATSFLSSPEIANYYHLHHSHLQQKHGKL 300

Query: 301 DEVAKRNIKMNSKKLIERGFIYKYDGDMILEDAFHCCKNQFVG 341
           DEVAKRNIKMNSKKLIERGFIYKYDGDMILED FHCCKNQF+G
Sbjct: 301 DEVAKRNIKMNSKKLIERGFIYKYDGDMILEDVFHCCKNQFLG 343

BLAST of CsGy3G008220 vs. NCBI nr
Match: XP_011650696.1 (putative anthocyanidin reductase isoform X1 [Cucumis sativus])

HSP 1 Score: 609 bits (1571), Expect = 1.06e-218
Identity = 298/337 (88.43%), Postives = 313/337 (92.88%), Query Frame = 0

Query: 5   SDCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNLVLFK 64
           S C RVCVTGGSGYVA+SL+K LL NGHIVHATLRNL DESKVGILK LPNAT NLVLF+
Sbjct: 2   SGCYRVCVTGGSGYVAASLVKNLLQNGHIVHATLRNLDDESKVGILKNLPNATTNLVLFE 61

Query: 65  ADIYEPHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELGTVRR 124
           ADIY+PHQFEAAI G H VFH+ATPL HTH +QY + TEASV A K IAK CVE GTVRR
Sbjct: 62  ADIYKPHQFEAAITGTHFVFHLATPLRHTHGSQYGNTTEASVTAMKMIAKFCVESGTVRR 121

Query: 125 LIYTASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITEKELLKF 184
           LIYTASIVSMSPMK DGSGFK+F DESCWTPLNLSYPFS+SFL GY++SKTITEKE LKF
Sbjct: 122 LIYTASIVSMSPMKGDGSGFKEFFDESCWTPLNLSYPFSDSFLQGYVESKTITEKEFLKF 181

Query: 185 GKSEESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEELDGKVP 244
           G+SEESK LEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEELDGKVP
Sbjct: 182 GESEESKRLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEELDGKVP 241

Query: 245 LVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHGKLDEVAKR 304
           LVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSS+EIANYYHLHHSHLQQKHGKLDEVAKR
Sbjct: 242 LVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSSEIANYYHLHHSHLQQKHGKLDEVAKR 301

Query: 305 NIKMNSKKLIERGFIYKYDGDMILEDAFHCCKNQFVG 341
           NIKMNSKKLIERGF+YKYDGDMILEDAFHCCKN+FVG
Sbjct: 302 NIKMNSKKLIERGFVYKYDGDMILEDAFHCCKNRFVG 338

BLAST of CsGy3G008220 vs. NCBI nr
Match: XP_031737949.1 (putative anthocyanidin reductase isoform X2 [Cucumis sativus])

HSP 1 Score: 596 bits (1536), Expect = 5.57e-214
Identity = 296/296 (100.00%), Postives = 296/296 (100.00%), Query Frame = 0

Query: 1   MAVSSDCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNL 60
           MAVSSDCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNL
Sbjct: 1   MAVSSDCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNL 60

Query: 61  VLFKADIYEPHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELG 120
           VLFKADIYEPHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELG
Sbjct: 61  VLFKADIYEPHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELG 120

Query: 121 TVRRLIYTASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITEKE 180
           TVRRLIYTASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITEKE
Sbjct: 121 TVRRLIYTASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITEKE 180

Query: 181 LLKFGKSEESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEELD 240
           LLKFGKSEESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEELD
Sbjct: 181 LLKFGKSEESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEELD 240

Query: 241 GKVPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHG 296
           GKVPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHG
Sbjct: 241 GKVPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHG 296

BLAST of CsGy3G008220 vs. ExPASy TrEMBL
Match: A0A0A0L371 (Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G119707 PE=3 SV=1)

HSP 1 Score: 687 bits (1774), Expect = 6.60e-250
Identity = 339/341 (99.41%), Postives = 341/341 (100.00%), Query Frame = 0

Query: 1   MAVSSDCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNL 60
           MAVSSDCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNL
Sbjct: 1   MAVSSDCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNL 60

Query: 61  VLFKADIYEPHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELG 120
           VLFKADIYEPHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELG
Sbjct: 61  VLFKADIYEPHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELG 120

Query: 121 TVRRLIYTASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITEKE 180
           TVRRLIYTASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITEKE
Sbjct: 121 TVRRLIYTASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITEKE 180

Query: 181 LLKFGKSEESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEELD 240
           LLKFGKSEESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEELD
Sbjct: 181 LLKFGKSEESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEELD 240

Query: 241 GKVPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHGKLDE 300
           GKVPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHGKLDE
Sbjct: 241 GKVPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHGKLDE 300

Query: 301 VAKRNIKMNSKKLIERGFIYKYDGDMILEDAFHCCKNQFVG 341
           VAKRNIKMNSKKLIERGF+YKYDGDMILEDAFHCCKN+FVG
Sbjct: 301 VAKRNIKMNSKKLIERGFVYKYDGDMILEDAFHCCKNRFVG 341

BLAST of CsGy3G008220 vs. ExPASy TrEMBL
Match: A0A5A7U5J5 (Vestitone reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G001230 PE=3 SV=1)

HSP 1 Score: 622 bits (1603), Expect = 8.23e-224
Identity = 307/343 (89.50%), Postives = 321/343 (93.59%), Query Frame = 0

Query: 1   MAVSSD--CLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATN 60
           MAVSS   CLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNL DESK+GILKRLPNAT 
Sbjct: 1   MAVSSSSGCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLDDESKIGILKRLPNATT 60

Query: 61  NLVLFKADIYEPHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVE 120
           NLVLFKADIYEPH+FEAAIRGCHIVFHVATPLHHTHATQY DVTEASVAAAK IAKLCVE
Sbjct: 61  NLVLFKADIYEPHEFEAAIRGCHIVFHVATPLHHTHATQYSDVTEASVAAAKMIAKLCVE 120

Query: 121 LGTVRRLIYTASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITE 180
           LGTVRRLIYTAS+VSMSPMK+DGSGFKD LDE+CWTPL LSYPFSNS +VGY+DSKT TE
Sbjct: 121 LGTVRRLIYTASVVSMSPMKDDGSGFKDVLDENCWTPLKLSYPFSNSTVVGYVDSKTATE 180

Query: 181 KELLKFGKSEESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEE 240
           KELLKFG+SEES  LEVV+L CGL+AG++ HPS A TT VTFSQFI ESEPFK LRFLEE
Sbjct: 181 KELLKFGESEESMKLEVVTLACGLIAGDTLHPSPAATTTVTFSQFIDESEPFKLLRFLEE 240

Query: 241 LDGKVPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHGKL 300
           LDGKVPLVHIDDVCEAHIFCMEQTSIHGRFLCA+SFLSS EIANYYHLHHSHLQQKHGKL
Sbjct: 241 LDGKVPLVHIDDVCEAHIFCMEQTSIHGRFLCATSFLSSPEIANYYHLHHSHLQQKHGKL 300

Query: 301 DEVAKRNIKMNSKKLIERGFIYKYDGDMILEDAFHCCKNQFVG 341
           DEVAKRNIKMNSKKLIERGFIYKYDGDMILED FHCCKNQF+G
Sbjct: 301 DEVAKRNIKMNSKKLIERGFIYKYDGDMILEDVFHCCKNQFLG 343

BLAST of CsGy3G008220 vs. ExPASy TrEMBL
Match: A0A1S3AUS8 (vestitone reductase-like OS=Cucumis melo OX=3656 GN=LOC103483203 PE=3 SV=1)

HSP 1 Score: 622 bits (1603), Expect = 8.23e-224
Identity = 307/343 (89.50%), Postives = 321/343 (93.59%), Query Frame = 0

Query: 1   MAVSSD--CLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATN 60
           MAVSS   CLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNL DESK+GILKRLPNAT 
Sbjct: 1   MAVSSSSGCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLDDESKIGILKRLPNATT 60

Query: 61  NLVLFKADIYEPHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVE 120
           NLVLFKADIYEPH+FEAAIRGCHIVFHVATPLHHTHATQY DVTEASVAAAK IAKLCVE
Sbjct: 61  NLVLFKADIYEPHEFEAAIRGCHIVFHVATPLHHTHATQYSDVTEASVAAAKMIAKLCVE 120

Query: 121 LGTVRRLIYTASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITE 180
           LGTVRRLIYTAS+VSMSPMK+DGSGFKD LDE+CWTPL LSYPFSNS +VGY+DSKT TE
Sbjct: 121 LGTVRRLIYTASVVSMSPMKDDGSGFKDVLDENCWTPLKLSYPFSNSTVVGYVDSKTATE 180

Query: 181 KELLKFGKSEESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEE 240
           KELLKFG+SEES  LEVV+L CGL+AG++ HPS A TT VTFSQFI ESEPFK LRFLEE
Sbjct: 181 KELLKFGESEESMKLEVVTLACGLIAGDTLHPSPAATTTVTFSQFIDESEPFKLLRFLEE 240

Query: 241 LDGKVPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHGKL 300
           LDGKVPLVHIDDVCEAHIFCMEQTSIHGRFLCA+SFLSS EIANYYHLHHSHLQQKHGKL
Sbjct: 241 LDGKVPLVHIDDVCEAHIFCMEQTSIHGRFLCATSFLSSPEIANYYHLHHSHLQQKHGKL 300

Query: 301 DEVAKRNIKMNSKKLIERGFIYKYDGDMILEDAFHCCKNQFVG 341
           DEVAKRNIKMNSKKLIERGFIYKYDGDMILED FHCCKNQF+G
Sbjct: 301 DEVAKRNIKMNSKKLIERGFIYKYDGDMILEDVFHCCKNQFLG 343

BLAST of CsGy3G008220 vs. ExPASy TrEMBL
Match: A0A5D3D2U9 (Vestitone reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G005360 PE=3 SV=1)

HSP 1 Score: 617 bits (1590), Expect = 7.88e-222
Identity = 305/343 (88.92%), Postives = 319/343 (93.00%), Query Frame = 0

Query: 1   MAVSSD--CLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATN 60
           MAVSS   CLRVCVT GSGYVASS IKKLLLNGHIVHATLRNL DESK+GILKRLPNAT 
Sbjct: 1   MAVSSSSGCLRVCVTCGSGYVASSFIKKLLLNGHIVHATLRNLDDESKIGILKRLPNATT 60

Query: 61  NLVLFKADIYEPHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVE 120
           NLVLFKADIYEPH+FEAAIRGCHIVFHVATPLHHTHATQY DVTEASVAAAK IAKLCVE
Sbjct: 61  NLVLFKADIYEPHEFEAAIRGCHIVFHVATPLHHTHATQYSDVTEASVAAAKMIAKLCVE 120

Query: 121 LGTVRRLIYTASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITE 180
           LGTVRRLIYTAS+VSMSPMK+DGSGFKD LDE+CWTPL LSYPFSNS +VGY+DSKT TE
Sbjct: 121 LGTVRRLIYTASVVSMSPMKDDGSGFKDVLDENCWTPLKLSYPFSNSTVVGYVDSKTATE 180

Query: 181 KELLKFGKSEESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEE 240
           KELLKFG+SEES  LEVV+L CGL+AG++ HPS A TT VTFSQFI ESEPFK LRFLEE
Sbjct: 181 KELLKFGESEESMKLEVVTLACGLIAGDTLHPSPAATTTVTFSQFIDESEPFKLLRFLEE 240

Query: 241 LDGKVPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHGKL 300
           LDGKVPLVHIDDVCEAHIFCMEQTSIHGRFLCA+SFLSS EIANYYHLHHSHLQQKHGKL
Sbjct: 241 LDGKVPLVHIDDVCEAHIFCMEQTSIHGRFLCATSFLSSPEIANYYHLHHSHLQQKHGKL 300

Query: 301 DEVAKRNIKMNSKKLIERGFIYKYDGDMILEDAFHCCKNQFVG 341
           DEVAKRNIKMNSKKLIERGFIYKYDGDMILED FHCCKNQF+G
Sbjct: 301 DEVAKRNIKMNSKKLIERGFIYKYDGDMILEDVFHCCKNQFLG 343

BLAST of CsGy3G008220 vs. ExPASy TrEMBL
Match: A0A0A0L5Y5 (Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G119705 PE=3 SV=1)

HSP 1 Score: 580 bits (1495), Expect = 2.34e-207
Identity = 288/342 (84.21%), Postives = 303/342 (88.60%), Query Frame = 0

Query: 5   SDCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNLVLFK 64
           S C RVCVTGGSGYVA+SL+K LL NGHIVHATLRNL DESKVGILK LPNAT NLVLF+
Sbjct: 2   SGCYRVCVTGGSGYVAASLVKNLLQNGHIVHATLRNLDDESKVGILKNLPNATTNLVLFE 61

Query: 65  ADIYEPHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELG---- 124
           ADIY+PHQFEAAI G H VFH+ATPL HTH +QY + TEASV                  
Sbjct: 62  ADIYKPHQFEAAITGTHFVFHLATPLRHTHGSQYGNTTEASVTXXXXXXXXXXXXXXXXX 121

Query: 125 -TVRRLIYTASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITEK 184
             VRRLIYTASIVSMSPMK DGSGFK+F DESCWTPLNLSYPFS+SFL GY++SKTITEK
Sbjct: 122 XAVRRLIYTASIVSMSPMKGDGSGFKEFFDESCWTPLNLSYPFSDSFLQGYVESKTITEK 181

Query: 185 ELLKFGKSEESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEEL 244
           E LKFG+SEESK LEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEEL
Sbjct: 182 EFLKFGESEESKRLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEEL 241

Query: 245 DGKVPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHGKLD 304
           DGKVPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSS+EIANYYHLHHSHLQQKHGKLD
Sbjct: 242 DGKVPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSSEIANYYHLHHSHLQQKHGKLD 301

Query: 305 EVAKRNIKMNSKKLIERGFIYKYDGDMILEDAFHCCKNQFVG 341
           EVAKRNIKMNSKKLIERGF+YKYDGDMILEDAFHCCKN+FVG
Sbjct: 302 EVAKRNIKMNSKKLIERGFVYKYDGDMILEDAFHCCKNRFVG 343

BLAST of CsGy3G008220 vs. TAIR 10
Match: AT5G42800.1 (dihydroflavonol 4-reductase )

HSP 1 Score: 155.6 bits (392), Expect = 7.1e-38
Identity = 106/333 (31.83%), Postives = 169/333 (50.75%), Query Frame = 0

Query: 10  VCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNLVLFKADIYE 69
           VCVTG SG++ S L+ +LL  G+ V AT+R+ G+  KV  L  LPNA   L L+KAD+ E
Sbjct: 8   VCVTGASGFIGSWLVMRLLERGYFVRATVRDPGNLKKVQHLLDLPNAKTLLTLWKADLSE 67

Query: 70  PHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELGTVRRLIYTA 129
              ++ AI GC  VFHVATP+         +V + +V     I K CV+  TVRR ++T+
Sbjct: 68  EGSYDDAINGCDGVFHVATPMDFESKDPENEVIKPTVNGMLGIMKACVKAKTVRRFVFTS 127

Query: 130 SIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVG--YLDSKTITEKELLKFGKS 189
           S  +++  +      K+  DE+ W+ L       +  + G  Y  SKT+ EK    F   
Sbjct: 128 SAGTVNVEEHQ----KNVYDENDWSDLEF---IMSKKMTGWMYFVSKTLAEKAAWDFA-- 187

Query: 190 EESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEELDGKVPLVH 249
            E KGL+ +S++  LV G     S   + +   S        +  +R       +   VH
Sbjct: 188 -EEKGLDFISIIPTLVVGPFITTSMPPSLITALSPITRNEAHYSIIR-------QGQYVH 247

Query: 250 IDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHS--HLQQKHGKLDEVAKRN 309
           +DD+C AHIF  EQ +  GR++C+S   +   I+ +    +   ++      +DE  K +
Sbjct: 248 LDDLCNAHIFLYEQAAAKGRYICSSHDATILTISKFLRPKYPEYNVPSTFEGVDENLK-S 307

Query: 310 IKMNSKKLIERGFIYKYDGDMILEDAFHCCKNQ 339
           I+ +SKKL + GF +KY  + +  ++   C+ +
Sbjct: 308 IEFSSKKLTDMGFNFKYSLEEMFIESIETCRQK 322

BLAST of CsGy3G008220 vs. TAIR 10
Match: AT2G45400.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 155.2 bits (391), Expect = 9.3e-38
Identity = 115/337 (34.12%), Postives = 171/337 (50.74%), Query Frame = 0

Query: 10  VCVTGGSGYVASSLIKKLLLNGHIVHATLR--NLGDESKVGILKRLPNATNNLVLFKADI 69
           VCVTGGSG+VAS LI +LL  G+ V AT+R  + G++  +  L  LP A+  L +F AD+
Sbjct: 40  VCVTGGSGFVASWLIMRLLQRGYSVRATVRTNSEGNKKDISYLTELPFASERLQIFTADL 99

Query: 70  YEPHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELGTVRRLIY 129
            EP  F+ AI GC  VFHVA P+          VT+ +V     I K C++  TV+R  Y
Sbjct: 100 NEPESFKPAIEGCKAVFHVAHPMDPNSNETEETVTKRTVQGLMGILKSCLDAKTVKRFFY 159

Query: 130 TASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNS----FLVGYLDSKTITEKELLK 189
           T+S V++     +G G  + +DES W+ + +   F N         Y+ SK   E   L+
Sbjct: 160 TSSAVTVFYSGGNGGGGGE-VDESVWSDVEV---FRNQKEKRVSSSYVVSKMAAETAALE 219

Query: 190 FGKSEESKGLEVVSLVCGLVAGESPHPSAAITTMVTFS---QFIHESEPFKFLRFLEELD 249
           FG      GLEVV+LV  LV G  P  S+++ + V  S    F +  E + F  +     
Sbjct: 220 FG---GKNGLEVVTLVIPLVVG--PFISSSLPSSVFISLAMLFGNYKEKYLFDTY----- 279

Query: 250 GKVPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHGKLDE 309
               +VHIDDV  A IF +E+    GR++C+S  +   E+  +        Q     L++
Sbjct: 280 ---NMVHIDDVARAMIFLLEKPVAKGRYICSSVEMKIDEVFEFLSTKFPQFQLPSIDLNK 339

Query: 310 V-AKRNIKMNSKKLIERGFIYKYDGDMILEDAFHCCK 337
              ++ + ++SKKL   GF +KY  + I   A   C+
Sbjct: 340 YKVEKRMGLSSKKLKSAGFEFKYGAEEIFSGAIRSCQ 359

BLAST of CsGy3G008220 vs. TAIR 10
Match: AT4G27250.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 151.4 bits (381), Expect = 1.3e-36
Identity = 98/329 (29.79%), Postives = 174/329 (52.89%), Query Frame = 0

Query: 11  CVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNLVLFKADIYEP 70
           CVTG SGY+ S L+K LL  G+ VHATLR+L   +K    +        L LF+AD+ + 
Sbjct: 14  CVTGASGYIGSWLVKSLLQRGYTVHATLRDL---AKSEYFQSKWKENERLRLFRADLRDD 73

Query: 71  HQFEAAIRGCHIVFHVATPLH------HTHATQYMD--VTEASVAAAKKIAKLCVELGTV 130
             F+ A++GC  VFHVA  +       H +   Y+   V E ++   + +   C++  +V
Sbjct: 74  GSFDDAVKGCDGVFHVAASMEFDISSDHVNLESYVQSKVIEPALKGVRNVLSSCLKSKSV 133

Query: 131 RRLIYTASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITEKELL 190
           +R+++T+SI +++  K++    + F+DE+C   ++       S  + Y+ SK ++E+E  
Sbjct: 134 KRVVFTSSISTLT-AKDENERMRSFVDETCKAHVDHVLKTQASGWI-YVLSKLVSEEEAF 193

Query: 191 KFGKSEESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEELDGK 250
           ++ K    +G+++VS++   V+G    P    +  V  S    +S+ F  L  + +  G 
Sbjct: 194 RYAK---ERGMDLVSVITTTVSGPFLTPFVPSSVQVLLSPITGDSKLFAILSAVNKRMGS 253

Query: 251 VPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHGKLDEVA 310
           + LVHI+D+C AH+F MEQ    G+++C    +   E+  ++       + +    DE  
Sbjct: 254 IALVHIEDICRAHLFLMEQPKAKGQYICCVDNIDMHELMLHHFSKDYLCKVQKVNEDEEE 313

Query: 311 KRNIK--MNSKKLIERGFIYKYDGDMILE 330
           +  +K  ++SKKL E GF YKY  + I++
Sbjct: 314 RECMKPIISSKKLRELGFEYKYGIEEIVD 334

BLAST of CsGy3G008220 vs. TAIR 10
Match: AT1G61720.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 147.1 bits (370), Expect = 2.5e-35
Identity = 90/314 (28.66%), Postives = 166/314 (52.87%), Query Frame = 0

Query: 9   RVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNLVLFKADIY 68
           + CV GG+G +AS LIK LL +G+ V+ T+R+  +E K+  L++L     +L +FKAD+ 
Sbjct: 12  KACVIGGTGNLASILIKHLLQSGYKVNTTVRDPENEKKIAHLRKL-QELGDLKIFKADLT 71

Query: 69  EPHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELGTVRRLIYT 128
           +   FE++  GC  +FHVATP++        D+ + ++     + K C++  +V+R+IYT
Sbjct: 72  DEDSFESSFSGCEYIFHVATPINFKSEDPEKDMIKPAIQGVINVLKSCLKSKSVKRVIYT 131

Query: 129 ASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITEKELLKFGKSE 188
           +S  ++S     G+G    ++E  WT +         F  GY  SK + EK   +F K  
Sbjct: 132 SSAAAVSINNLSGTGI--VMNEENWTDVEF-LTEEKPFNWGYPISKVLAEKTAWEFAKEN 191

Query: 189 ESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEELDGKVPLVHI 248
           +   + +V+++  L+AG S       +  ++ S    +      L+ +++L G +  VH+
Sbjct: 192 K---INLVTVIPALIAGNSLLSDPPSSLSLSMSFITGKEMHVTGLKEMQKLSGSISFVHV 251

Query: 249 DDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHGKLDEVAKRNIKM 308
           DD+  AH+F  E+ +  GR++C +   S  EIA++    +          + ++   + +
Sbjct: 252 DDLARAHLFLAEKETASGRYICCAYNTSVPEIADFLIQRYPKYNVLSEFEEGLSIPKLTL 311

Query: 309 NSKKLIERGFIYKY 323
           +S+KLI  GF ++Y
Sbjct: 312 SSQKLINEGFRFEY 318

BLAST of CsGy3G008220 vs. TAIR 10
Match: AT1G09510.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 140.2 bits (352), Expect = 3.1e-33
Identity = 95/271 (35.06%), Postives = 140/271 (51.66%), Query Frame = 0

Query: 10  VCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNLVLFKADIYE 69
           VCVTG SGYVAS ++K LLL G+ V AT+R+  DE K   L  L  A   L LFKAD+ E
Sbjct: 8   VCVTGASGYVASWIVKLLLLRGYTVRATVRDPSDEKKTEHLLALDGAKEKLKLFKADLLE 67

Query: 70  PHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELGTVRRLIYTA 129
              FE AI GC  VFH A+P+  T     +++ + +V     + K C ++ +V+R+I T+
Sbjct: 68  EGSFEQAIEGCDAVFHTASPVSLTVTDPQIELIDPAVKGTLNVLKTCAKVSSVKRVIVTS 127

Query: 130 SIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITEKELLKFGKSEE 189
           S+ ++   +E   G  D +DESC++  N    F     + Y  SKT+ E E  +F K   
Sbjct: 128 SMAAVL-FREPTLGPNDLVDESCFSDPN----FCTEKKLWYALSKTLAEDEAWRFAK--- 187

Query: 190 SKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEELDGKVPLVHID 249
            KGL++V +  GLV G    PS   +  V   + I   + F        ++    LV + 
Sbjct: 188 EKGLDLVVINPGLVLGPLLKPSLTFSVNV-IVELITGKDNF--------INKDFRLVDVR 247

Query: 250 DVCEAHIFCMEQTSIHGRFLCASSFLSSTEI 281
           DV  AHI   E  S +GR++     ++  +I
Sbjct: 248 DVALAHIKAFETPSANGRYIIEGPVVTINDI 261

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5XLY01.4e-4332.14Putative anthocyanidin reductase OS=Ginkgo biloba OX=3311 PE=2 SV=1[more]
Q403169.7e-4033.93Vestitone reductase OS=Medicago sativa OX=3879 PE=1 SV=1[more]
Q7PCC41.1e-3830.37Anthocyanidin reductase ((2S)-flavan-3-ol-forming) OS=Vitis vinifera OX=29760 GN... [more]
D7U6G61.4e-3830.67Anthocyanidin reductase ((2S)-flavan-3-ol-forming) OS=Vitis vinifera OX=29760 GN... [more]
P511031.4e-3830.82Dihydroflavonol 4-reductase OS=Callistephus chinensis OX=13379 GN=F PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_004134310.33.35e-25099.71putative anthocyanidin reductase isoform X1 [Cucumis sativus] >KAE8650235.1 hypo... [more]
XP_008437918.11.70e-22389.50PREDICTED: vestitone reductase-like [Cucumis melo] >KAA0048905.1 vestitone reduc... [more]
TYK17664.11.63e-22188.92vestitone reductase-like [Cucumis melo var. makuwa][more]
XP_011650696.11.06e-21888.43putative anthocyanidin reductase isoform X1 [Cucumis sativus][more]
XP_031737949.15.57e-214100.00putative anthocyanidin reductase isoform X2 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A0A0L3716.60e-25099.41Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G119707 P... [more]
A0A5A7U5J58.23e-22489.50Vestitone reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
A0A1S3AUS88.23e-22489.50vestitone reductase-like OS=Cucumis melo OX=3656 GN=LOC103483203 PE=3 SV=1[more]
A0A5D3D2U97.88e-22288.92Vestitone reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A0A0L5Y52.34e-20784.21Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G119705 P... [more]
Match NameE-valueIdentityDescription
AT5G42800.17.1e-3831.83dihydroflavonol 4-reductase [more]
AT2G45400.19.3e-3834.12NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G27250.11.3e-3629.79NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G61720.12.5e-3528.66NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G09510.13.1e-3335.06NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 8..340
e-value: 6.4E-77
score: 261.2
NoneNo IPR availablePANTHERPTHR10366NAD DEPENDENT EPIMERASE/DEHYDRATASEcoord: 9..333
NoneNo IPR availablePANTHERPTHR10366:SF696DIHYDROFLAVONOL-4-REDUCTASE-LIKE PROTEINcoord: 9..333
IPR001509NAD-dependent epimerase/dehydratasePFAMPF01370Epimerasecoord: 10..261
e-value: 9.9E-15
score: 54.7
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 9..282

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy3G008220.2CsGy3G008220.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006694 steroid biosynthetic process
molecular_function GO:0003854 3-beta-hydroxy-delta5-steroid dehydrogenase activity
molecular_function GO:0003824 catalytic activity