CsGy3G001750 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy3G001750
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionbeta-amyrin 11-oxidase-like
LocationGy14Chr3: 1301968 .. 1308449 (-)
RNA-Seq ExpressionCsGy3G001750
SyntenyCsGy3G001750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTTGAAGATGATGATGAATTGGTTGGTGTTGATATTTGGAGGTTTAGTTTTAGTTTTCGGAGTTTTGAAGAGATTGAATGATTGGTATTATGAAGTTAAGCTTGGGAAACTATGGCCTAAGCTTCCTCCTGGTCATATGGGTTGGCCTTTTGTTGGTTCTACTCTATCTTTCATCAAAGATTACACTTCTGGTCAACCTCAGAATTTCGTTAAAGCCCTTCAGATCAGGTTGCATTTGATCTCCCAACTCTTTTTTTGTTGTTGTTTTTCAGTTTAATTATATGTGTCGATTCTATCCTTTTTTTATTATCGAGATCAATATTACATTTTTTTAGTACAATAATTAAAGCTATGTTTATATTGGTTGTTTGGTTTTTGTATCTAAAATTTGTGCATCTTTGTTCAAATAACTGTGTGTTTAAAACTCGAATTTGAGTTTATAGTTTAATTTGTTTGATAGATTACACCTTTGTTTTTAAGTCTCTTGTGTTGGTTTGAATTTCAATATCAATACAAAACTTAGTTCTTAAATATATTTAATTTTTTAAAATTTTATCTTGAATCGAACTAATTAAGGATGAAAATAAAACTTCCCATGGGGTCGGGTCTCCGCAGGTACCTATCCCTTAATGGGGTGGGGCGTGGATCAAACCGAGGAGAAAATATGGGTTTCGTGGGAGGGTATCTCTTCCCTCAAATATTTTCTAAATCTTTTTAATATAATGTTATTGGGACATTTTAGAAAATAACAAAATATTGAAACTATTTATAAAATGTAGCCAAATTTATTCATCGCTCAATATTTTATTTTTAAAAAAATATTTTTGTTATATTTAAAAATATTAAAACTATTTATAAAATTGATAAAAATAAATAATTTTAATATTTTACTATTCATAACTCTTTCCATTTCCTTTTTTTAAAAAAAAAATAAATATATTACAATTGTTAAAGCTACTATTTATATATTAACCTTTCTCTTTCAACTTTTCTTTTAAATTTCATTTCATAATATAATTAATTTGAACTTTTTATTATTTTTTATTAAAATAAACAAATAATCTTTTTTAATTATTAATGTTAATAAGTAAATAAAAATTTTAAAATAAATTATACATAGAAACTATTTTGAAGAGGAGGGATTCCTCGACCCGACCTTCCAAAATGGGGAATGGGGTCAGGGTGGAGAATTACAAATCCCCAAAGGGGACGATGAGGAAGTAGAGAGACTAATAGACTCAAATCGCGCAAGGTCTCATTAGCATCGGAAAGGAAATGCATCATAGCGGTGTCATAAAAGACGCCATCGATGAATTGAGTGCCAAGGAGGAGGAAGTCGTTGTTCCATTTGAAGAAACAAGGGGAGATTTCGGGGATGAAGGTACTGAAGAAGATGAGAATGAGGCAAGTGATCTTAATGAGGACGAGAGGTTTAGAGTAGAGGTTATTAGGGGAAGGGTTGTCTCCGACAGCGAAGTCATCATCGTTGGAGGGACTACTGTGGGCGATGGCGGAGGAGAGGAGGAGGAGGGAAGAGGTTAGAGAGAAAGGGAGAAGAAATGTAGAGGTTTGGGTGAAGTTTGGCATGGGAGACTAGGGCTTTCGTGCATGGGAGACTAGGGCTTTTCTAGAATTTGGAGTGATAAAAGGAAATTTTGGGGAAAAAAAAGAGGGAAAAGTTTAGTTAGACAAATTGGATAAAATTGAGAAATTTGAAAAAAAAGGAAGAGTGTTTTAGAAAATGGATAAAAAATATGTTACTTCACATTTTTTAAAGGGAAATTTTTAAAAATAATAGATTTTACAAAATATTTACATCCTATAGCAAATTCTATCATTCATCATTAATATGCTACAGTGACAAGACTATCAGTAATAAAATCCAAAATTTTGTTATACCTTGTAAATATTTTAATTTATTTTGTTGTTTTTGAAAATGCCCATTTTTTAAATGTCAAGTAATTAAAATTAATCTGGACATTTTTAATCTACCAAATATTTAAAATTTAAAAGAAAATTATGCATTTTTTTATTATGAGTTATTATTGACATTTTTTTCTTCTACTTGACAGTTTTTATTAAAACTATAAAAAATATCAAAACTGCAAGCATTAAGTATGGTCAGTTTTGTAATAATGGATTGAGATCATGTGAACGTGTACTCTCGCACTAGTTTTAAATTATTGTTTTATTTTTGGTCGTCAGAAAGTTTCCTATTTAGTTTAATTTTATAAGGATTTACATTGAGGGAGAAAAATAAGATAAAAAATTCACAGAAATAAGAGAAGAATGACTAATAAAATCAAAATTTGCCTCGAGCACACGATAAGTGTATTCTCCTATCATCATTATCAATCTTATATTTCTTTAAGAAAAAAAGATGTGCCAAAGCATAGGATCAGTAGATGTACGAAAGATACTCTCAATCAGGGTTGACACATATTCATAGCAATCTCATGATCATATGTTTGGATGCCAAAATCTATACCATATTATCAGTTATCATAAATCAAATACAAAGCAAGCAAGAGCAGAGCTTAAAGAAAATAAAATAACAATATAAATTGTGTGTTTTTTTTCTTTTTTTATGATAATTTCATATATTTATAATAAAATGTTTATTTTTACTATAGATATGGGAAAGCTGATATGTACAAAACTCACTTACTTGGGAGGCCAAGTATTATTGTTTACACACCTGAGATCTGTCGTCAAGTCTTGACAAATGAAGAAATATTCACTCCTAGTTTACCCAAAAACATAGTAATTTTATCTGGTAGAAAATCACTTATGCAAGTCACCAAGGCCGAGCACCGGCGCCTACGGCGGCTCACAACAGCCCCGATCAGCAGCCACACCGCGCTCGAGATTTATATCAATGATATTGAGCAAACAGTGATGAATGGGTTAGAGGAATGGGCAAGCATGAAGTCCCCAATTGAGCTATTGACTGAGATGAAGAAGTTAACTTTCAAAGTTATTTGGAACATTTTTATGGGTTCAACCTCTTCCATGGGAGAAAATGAGAGTTCTTTGTTTTATGAAGTTGCTGCGGGATTTTTGTCTTTGCCCATCAACTTTCCTGGCTTTGGCTTTCGCAAGTCCTTCAAGGTAAATCAAACTTTATCCTTTTTTACAAGTTAAATTGCATGTTTACTTGTTCCTTTAATTTAAAAAGACTTAAGCCAGATAGGATGAGGATGTACTGAGACTGATAACTTCTCTTTATTCATGTTCCTATTCCTACTGGCTTTAAACTTATTTCATTTGATTCTTTGTTCCTCTCAAATATTTTGAACTTTCGTTTGACAAGTTTTTATGTTTAGTAAATCTTTAAAGTTTAAAATATATATCTAATAATAAAAGTTTTTCAAACTTTTGATATGGTGTCTAATAGTATACTAATTAAACGTTTAAAATGTTAAATAACTTGTTCTTGAACAATTTTCAATTTTATGTTTAAATAGGTTTGTGACATATTTTTTTAACTAAACATAGTGTTAAACTCTTATACTCTTACGTTAATACTAGATGTGTGAAGTTTAAAAGTGACCAAAAAGTTTTTACATTCTGAACTCCCAACGGTTTCATGGGCTAGGCTCCAAGAGTTCACAATAATACTCGAAGAACTTGATAAACCTCTTTGAAGTTTCACAATAACTTCATTCCTTCCTGGAAACCTAAGTTTAATTAGGAACTTCTATGATATCATATATATATTTAACCTTGAATATTATTCCTTGTTATATAATATATATAGGCACGACAAAAGTTGATGGAAAGACTTCAATGTATCATCAATGAGAAGAGATCAATGAAAGAGAGGAAAGGGGAAAATTGGGAAGCAAAAGATACGATGGATTTTCTAATAGATGTGAAAGATGAAGATGGTGAAGAGTTGGATGATGAGACCATTAGAGATTTGATATTTGGTAAGTTATTTGCAGGCCATGAAACTACAGCTTACACTGCTATGTGGGCTGTTTTGTTTCTTACAGATCATCCACATACATTTCAGAAGGCGAAGGTAAAGTAATTTAATTAGTGTTCCTAATTTCTCCTAACTTTATTAATAATATCATCCACATCGATACCATTGTTTATCAAATTATTTTCGTCTAAACCCTAAATTCAAAATTCAAATTTAATGACATCGCTTTTGATGTTAAGACATACATGATGAGGATACTAGATAGATGTCAACCTAGTTAATATAGCATCTTCTAATCTAATCCATCTTGTTGCGTTGTGAATATTTTCAAAAGACAAAGAAACAATGACTAAACATTATATTTAATTCAACACTTGTAAATGCAAAGTCACTCCTTTTTCATCAATATTTAATTATCGCAATATCTATCAATGATTTTGTGGTTGAATATTTTAGGAAGAACAAGAAGAGCTTATTAGAAGAAGACCCTCAACTCAAAAGGGTATAAATTTCTCCGAATTTAAACAAATGAAATATCTTTCTCAGGTATATACATTTTCTTTTATTTTTTTTCTTTTAATTTTGCCATTTACTTTATAAAGTTTAAAGAAAGAAAGAAATAAAACTATTTCAAATTATTAAAATGGCCAAAAAATTGTCTCCCTTTTATAATTTGGCTAATATTTTTAAACATATGATCAAAGTTAAGTAATAAAGAAAAATGGTTAGAACATTATTAGATTTTATTGTATTAATTTTCAGGTGATTGACGAGACACTTCGTGTTGGTAGTATCACATCTTTGCTTTATCGTGAGACAACGATTGATGTCGAAATTAATGGTTAGTTATCAAAGTTAATATCTATATAGTTATATGATATTTACCACATATAACGATTCTATTTATATACTATAGTTATATTTATTTATTAAGTACATTTGATGATTTATTATAATAGTTGAAAAATTATTTATGATCTCTGTTAAAATTATTAGGTTATATATCTAACTACTAAAGAATGTGTGTGTGATATAGTAAATTAAATTTCAAAAGGCTTTTGATGTATAAAGATAATATATTTTAATTAATTTAGGGAAAATATAAAAATTCATAATATAGCATTTTAATTGGCTTTTAATTATTATACAATTATTTGCATTTTCATTTTTATTCTTTTAGGTAAAATTATACCAAAGGGATGGAAAGTATTACCTTGGCTATCTGAGCTTTATGTGAATGAAACAACATTTTCTTCCCCACAAGATTTTAACCCTTCAAGATGGGATGTAAGTATTTATCCAATACTAATACCTTTTCATTCCCTAAATATGTTTCCATTTTTAATGTGACATATGTTTGGACACTATCATATTAGCCTCAATTTTAACTTCAACTTCAACTTTGTCTATATCATATATATAACTTAAAAGTTAGTGTATCTCTCCATTCAATATTCACCTGTGTTAAAGTATTTTGTGAATGTGAGATAAAATTTCTATCGATATACAAGAGATCAAATTATAAACTCGTGTTCAACTCTAAGCTAGAGTTCGTAATGTTCTCTTTTTAATTCTATTTAAATGTCATACACCAACAAAAGTTGTTGCACGTTGTTGATTTATAAGTTTTTAACTATACATTGAGTCGTTTTTAAAGTGATTAGTCTAGTTTTCTTCGGTTCTAAGTTCTCATTTATTGTTCTAAGTAGTTTGAAGTTTCCTATGTGAAGACCCAATTAAAGATATAATCTTATCAATGATAAACTTCTATTATTTAATTAAGAAATTAATTGTAAATATAATCATCTTAGTGATAGGCTTCTATTAGAGATGTGGTCTATCACTAATAGACAATGAAAGTTGAACCAAATTTTGTCATATACAAATACACGTGATATTTTATGTTCATTTGTAATGGTATTTTATGGTATTTTGGTTTGATTTTTCTCAACAGAATACTAGAGTCAAACCAGGAGCTTTTGTTCCTTTTGGATTAGGAAACAGGTTGTGCCCTGGCAGTGATCTGGCCAAGCTCGAGATCTCAATTTTTCTTCATCATTTTCTCCTCAATTACAAGTGAGTTTGTTTTATGAATAATATAATATTCATTTCATACTAGTAACTTATAATAATACACATTATAAATATACTTGTTTTTAAATATAGCAAAACGAACCAGCAACAATTTAGAATCATTATCAATAATAGATACTTCTATCAATGTAGACACCGATATAAGTCTATTTGTGTTAATTATTAATAAATTTAGAAATTTTTATATTTGAAATAATTCTTCTTATAAATATATCAAAATTTGAAAAAGAATATCATATATAAATATATATACAATATTCTTTTTTTATATAGAAATGGGAGGGATTAAACTAACTATTTATCTTAAAAATTGCATATTCGTTATTCTATCTCTACTAAACAAAACTTTCGAATGCAGGGTGGAACGACTTAATCCAAAATCCAAATTGACTTACGCACCATTCCCTCATCCTAAGGACAAATGTTTGGCGAGAGTGTTCAAAGTTGAGTAA

mRNA sequence

ATGGAGTTGAAGATGATGATGAATTGGTTGGTGTTGATATTTGGAGGTTTAGTTTTAGTTTTCGGAGTTTTGAAGAGATTGAATGATTGGTATTATGAAGTTAAGCTTGGGAAACTATGGCCTAAGCTTCCTCCTGGTCATATGGGTTGGCCTTTTGTTGGTTCTACTCTATCTTTCATCAAAGATTACACTTCTGGTCAACCTCAGAATTTCGTTAAAGCCCTTCAGATCAGATATGGGAAAGCTGATATGTACAAAACTCACTTACTTGGGAGGCCAAGTATTATTGTTTACACACCTGAGATCTGTCGTCAAGTCTTGACAAATGAAGAAATATTCACTCCTAGTTTACCCAAAAACATAGTAATTTTATCTGGTAGAAAATCACTTATGCAAGTCACCAAGGCCGAGCACCGGCGCCTACGGCGGCTCACAACAGCCCCGATCAGCAGCCACACCGCGCTCGAGATTTATATCAATGATATTGAGCAAACAGTGATGAATGGGTTAGAGGAATGGGCAAGCATGAAGTCCCCAATTGAGCTATTGACTGAGATGAAGAAGTTAACTTTCAAAGTTATTTGGAACATTTTTATGGGTTCAACCTCTTCCATGGGAGAAAATGAGAGTTCTTTGTTTTATGAAGTTGCTGCGGGATTTTTGTCTTTGCCCATCAACTTTCCTGGCTTTGGCTTTCGCAAGTCCTTCAAGGCACGACAAAAGTTGATGGAAAGACTTCAATGTATCATCAATGAGAAGAGATCAATGAAAGAGAGGAAAGGGGAAAATTGGGAAGCAAAAGATACGATGGATTTTCTAATAGATGTGAAAGATGAAGATGGTGAAGAGTTGGATGATGAGACCATTAGAGATTTGATATTTGGTAAGTTATTTGCAGGCCATGAAACTACAGCTTACACTGCTATGTGGGCTGTTTTGTTTCTTACAGATCATCCACATACATTTCAGAAGGCGAAGGAAGAACAAGAAGAGCTTATTAGAAGAAGACCCTCAACTCAAAAGGGTATAAATTTCTCCGAATTTAAACAAATGAAATATCTTTCTCAGGTGATTGACGAGACACTTCGTGTTGGTAGTATCACATCTTTGCTTTATCGTGAGACAACGATTGATGTCGAAATTAATGGTAAAATTATACCAAAGGGATGGAAAGTATTACCTTGGCTATCTGAGCTTTATGTGAATGAAACAACATTTTCTTCCCCACAAGATTTTAACCCTTCAAGATGGGATAATACTAGAGTCAAACCAGGAGCTTTTGTTCCTTTTGGATTAGGAAACAGGTTGTGCCCTGGCAGTGATCTGGCCAAGCTCGAGATCTCAATTTTTCTTCATCATTTTCTCCTCAATTACAAGGTGGAACGACTTAATCCAAAATCCAAATTGACTTACGCACCATTCCCTCATCCTAAGGACAAATGTTTGGCGAGAGTGTTCAAAGTTGAGTAA

Coding sequence (CDS)

ATGGAGTTGAAGATGATGATGAATTGGTTGGTGTTGATATTTGGAGGTTTAGTTTTAGTTTTCGGAGTTTTGAAGAGATTGAATGATTGGTATTATGAAGTTAAGCTTGGGAAACTATGGCCTAAGCTTCCTCCTGGTCATATGGGTTGGCCTTTTGTTGGTTCTACTCTATCTTTCATCAAAGATTACACTTCTGGTCAACCTCAGAATTTCGTTAAAGCCCTTCAGATCAGATATGGGAAAGCTGATATGTACAAAACTCACTTACTTGGGAGGCCAAGTATTATTGTTTACACACCTGAGATCTGTCGTCAAGTCTTGACAAATGAAGAAATATTCACTCCTAGTTTACCCAAAAACATAGTAATTTTATCTGGTAGAAAATCACTTATGCAAGTCACCAAGGCCGAGCACCGGCGCCTACGGCGGCTCACAACAGCCCCGATCAGCAGCCACACCGCGCTCGAGATTTATATCAATGATATTGAGCAAACAGTGATGAATGGGTTAGAGGAATGGGCAAGCATGAAGTCCCCAATTGAGCTATTGACTGAGATGAAGAAGTTAACTTTCAAAGTTATTTGGAACATTTTTATGGGTTCAACCTCTTCCATGGGAGAAAATGAGAGTTCTTTGTTTTATGAAGTTGCTGCGGGATTTTTGTCTTTGCCCATCAACTTTCCTGGCTTTGGCTTTCGCAAGTCCTTCAAGGCACGACAAAAGTTGATGGAAAGACTTCAATGTATCATCAATGAGAAGAGATCAATGAAAGAGAGGAAAGGGGAAAATTGGGAAGCAAAAGATACGATGGATTTTCTAATAGATGTGAAAGATGAAGATGGTGAAGAGTTGGATGATGAGACCATTAGAGATTTGATATTTGGTAAGTTATTTGCAGGCCATGAAACTACAGCTTACACTGCTATGTGGGCTGTTTTGTTTCTTACAGATCATCCACATACATTTCAGAAGGCGAAGGAAGAACAAGAAGAGCTTATTAGAAGAAGACCCTCAACTCAAAAGGGTATAAATTTCTCCGAATTTAAACAAATGAAATATCTTTCTCAGGTGATTGACGAGACACTTCGTGTTGGTAGTATCACATCTTTGCTTTATCGTGAGACAACGATTGATGTCGAAATTAATGGTAAAATTATACCAAAGGGATGGAAAGTATTACCTTGGCTATCTGAGCTTTATGTGAATGAAACAACATTTTCTTCCCCACAAGATTTTAACCCTTCAAGATGGGATAATACTAGAGTCAAACCAGGAGCTTTTGTTCCTTTTGGATTAGGAAACAGGTTGTGCCCTGGCAGTGATCTGGCCAAGCTCGAGATCTCAATTTTTCTTCATCATTTTCTCCTCAATTACAAGGTGGAACGACTTAATCCAAAATCCAAATTGACTTACGCACCATTCCCTCATCCTAAGGACAAATGTTTGGCGAGAGTGTTCAAAGTTGAGTAA

Protein sequence

MELKMMMNWLVLIFGGLVLVFGVLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSFIKDYTSGQPQNFVKALQIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPKNIVILSGRKSLMQVTKAEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPIELLTEMKKLTFKVIWNIFMGSTSSMGENESSLFYEVAAGFLSLPINFPGFGFRKSFKARQKLMERLQCIINEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKLFAGHETTAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQVIDETLRVGSITSLLYRETTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSRWDNTRVKPGAFVPFGLGNRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPHPKDKCLARVFKVE*
Homology
BLAST of CsGy3G001750 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 1.3e-138
Identity = 245/490 (50.00%), Postives = 345/490 (70.41%), Query Frame = 0

Query: 5   MMMNWLVLIFGGLVL--VFG--VLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSFI 64
           M ++W+ +    L++  +FG   ++ LN WYY+VKL +    LPPG MGWP +G  LSFI
Sbjct: 1   MEVHWVCMSAATLLVCYIFGSKFVRNLNGWYYDVKLRRKEHPLPPGDMGWPLIGDLLSFI 60

Query: 65  KDYTSGQPQNFVKALQIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPKN 124
           KD++SG P +F+  L ++YG++ +YKTHL G PSIIV  P++CR+VLT++  F    PK+
Sbjct: 61  KDFSSGHPDSFINNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKS 120

Query: 125 IVILSGRKSLMQVTKAEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPI 184
           I  L+  + ++ V+ AEHR  RRL T+PI  H AL +Y+  +E+ V+N LEE +SMK P+
Sbjct: 121 IKELARCRPMIDVSNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPV 180

Query: 185 ELLTEMKKLTFKVIWNIFMGSTS-SMGENESSLFYEVAAGFLSLPINFPGFGFRKSFKAR 244
           ELL EMKK++FK I ++FMGS++  + +   S F ++  G  S+PIN PGF F K+ +AR
Sbjct: 181 ELLKEMKKVSFKAIVHVFMGSSNQDIIKKIGSSFTDLYNGMFSIPINVPGFTFHKALEAR 240

Query: 245 QKLMERLQCIINEKRSMKER-KGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKLF 304
           +KL + +Q +++E+R M E    E  + KD +D L++VKDE+G +L+DE I DL+ G LF
Sbjct: 241 KKLAKIVQPVVDERRLMIENGPQEGSQRKDLIDILLEVKDENGRKLEDEDISDLLIGLLF 300

Query: 305 AGHETTAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQVI 364
           AGHE+TA + MW++ +LT HPH  +KAKEEQEE+ R R S+QK ++  E KQM YLSQVI
Sbjct: 301 AGHESTATSLMWSITYLTQHPHILKKAKEEQEEITRTRFSSQKQLSLKEIKQMVYLSQVI 360

Query: 365 DETLRVGSITSLLYRETTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSRWD 424
           DETLR  +I    +RE T DV ING IIPKGW+VL W   ++++   + +P++FNPSRWD
Sbjct: 361 DETLRCANIAFATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPSRWD 420

Query: 425 NTRVKPGAFVPFGLGNRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPHPK 484
           +   K G F+PFG G+RLCPG+DLAKLEISIFLH+FL NY++ER+NP+  +T  P   P 
Sbjct: 421 DYNAKAGTFLPFGAGSRLCPGADLAKLEISIFLHYFLRNYRLERINPECHVTSLPVSKPT 480

Query: 485 DKCLARVFKV 489
           D CLA+V KV
Sbjct: 481 DNCLAKVIKV 490

BLAST of CsGy3G001750 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 2.5e-121
Identity = 217/483 (44.93%), Postives = 319/483 (66.05%), Query Frame = 0

Query: 9   WLVLIFGGLVLVFGVLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSFIKDYTSGQP 68
           W  L+  G   +  +++++N W YE  LG+    LPPG +GWPF+G+ LSF++ + +  P
Sbjct: 11  WFPLMVLGCFGLNWLVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDP 70

Query: 69  QNFVKALQIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPKNIVILSGRK 128
            +F + L  RYG   +YK H+ G PSIIV T + CR+VLT+++ F P  P + + L GRK
Sbjct: 71  DSFTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRK 130

Query: 129 SLMQVTKAEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPIELLTEMKK 188
           S + ++  EH+RLRRLT AP++ H AL  YI  IE+ V+  L++W  M    E LT ++K
Sbjct: 131 SFVGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKM-GEFEFLTHLRK 190

Query: 189 LTFKVIWNIFMGSTS-----SMGENESSLFYEVAAGFLSLPINFPGFGFRKSFKARQKLM 248
           LTF++I  IF+ S S     ++    ++L Y V A    + +N PGF + ++ KAR+ L+
Sbjct: 191 LTFRIIMYIFLSSESENVMDALEREYTALNYGVRA----MAVNIPGFAYHRALKARKTLV 250

Query: 249 ERLQCIINEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKLFAGHET 308
              Q I+ E+R+ +++   +   KD +D L++VKDEDG+ LDDE I D++   L AGHE+
Sbjct: 251 AAFQSIVTERRNQRKQNILS-NKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHES 310

Query: 309 TAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQVIDETLR 368
           + +T MWA +FL +HP   Q+AK EQE +++ RP  QKG++  E ++M++LSQV+DETLR
Sbjct: 311 SGHTIMWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLR 370

Query: 369 VGSITSLLYRETTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSRWDNTRV- 428
           V + +   +RE   DVE+NG +IPKGWKVL W  +++++   F  P+ F+P+RWDN  V 
Sbjct: 371 VITFSLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVP 430

Query: 429 KPGAFVPFGLGNRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPHPKDKCL 486
           K GAF+PFG G+ LCPG+DLAKLEISIFLHHFLL Y+V+R NP+  + Y P   P D CL
Sbjct: 431 KAGAFLPFGAGSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCL 487

BLAST of CsGy3G001750 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 437.2 bits (1123), Expect = 2.5e-121
Identity = 223/489 (45.60%), Postives = 321/489 (65.64%), Query Frame = 0

Query: 2   ELKMMMNWLVLIFGGLVLVFGVLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSFIK 61
           E  +++ W  LI  GL ++  VLKR+N W Y  KLG+    LPPG +GWP +G+  SF++
Sbjct: 3   ETGLILMWFPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLR 62

Query: 62  DYTSGQPQNFVKALQIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPKNI 121
            + +  P++F+++   RYG+  +YK H+ G P ++V TPE CR+VLT+++ F    PK+ 
Sbjct: 63  AFKTSDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKST 122

Query: 122 VILSGRKSLMQVTKAEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPIE 181
           + L GRKS + ++  EH+RLRRLT+AP++   AL +YI  IE+TV   LE+W+ M   IE
Sbjct: 123 MKLIGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM-GEIE 182

Query: 182 LLTEMKKLTFKVIWNIFMGSTS-----SMGENESSLFYEVAAGFLSLPINFPGFGFRKSF 241
            L+ ++KLTFKVI  IF+ S S     S+    ++L Y V A    + IN PGF + ++ 
Sbjct: 183 FLSHLRKLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVRA----MGINLPGFAYHRAL 242

Query: 242 KARQKLMERLQCIINEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGK 301
           KAR+KL+   Q I+  +R+ +++   +   KD +D LIDVKDE+G  LDDE I DL+   
Sbjct: 243 KARKKLVAAFQSIVTNRRNQRKQNISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMY 302

Query: 302 LFAGHETTAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQ 361
           L AGHE++ +  MWA + + +HP   QKAKEEQE ++++R   QK +   E ++M YLSQ
Sbjct: 303 LNAGHESSGHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQ 362

Query: 362 VIDETLRVGSITSLLYRETTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSR 421
           VIDETLRV + +   +RE   DV+++G IIPKGWKVL W   ++++   +  P+ F+PSR
Sbjct: 363 VIDETLRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSR 422

Query: 422 WDNTRVKPGAFVPFGLGNRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPH 481
           W+    K G F+PFGLG+ LCPG+DLAKLEISIFLHHFLL Y+VER NP   + + P   
Sbjct: 423 WEGYTPKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNR 482

Query: 482 PKDKCLARV 486
           PKD CLAR+
Sbjct: 483 PKDNCLARI 484

BLAST of CsGy3G001750 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 1.8e-116
Identity = 207/479 (43.22%), Postives = 313/479 (65.34%), Query Frame = 0

Query: 13  IFGGLVLVFGVLKRLNDWYYEVKLG-KLWPKLPPGHMGWPFVGSTLSFIKDYTSGQPQNF 72
           +  G+VLV  V++R +DW     LG +   +LPPG MGWP VGS  +F++ + SG P  F
Sbjct: 24  VVAGVVLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFKSGNPDAF 83

Query: 73  VKALQIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPKNIVILSGRKSLM 132
           + +   R+G+  +Y+T +   P+I+  TPE C+QVL ++E F    PK  V L G KS +
Sbjct: 84  IASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVTLIGPKSFV 143

Query: 133 QVTKAEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKS-PIELLTEMKKLT 192
            ++  +HRR+R+LT API+   AL  Y++ I+QTV+  L  W+S +S  +E LTE++++T
Sbjct: 144 NMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEFLTELRRMT 203

Query: 193 FKVIWNIFM-GSTSSMGENESSLFYEVAAGFLSLPINFPGFGFRKSFKARQKLMERLQCI 252
           FK+I  IFM G+  +  E     + ++  G  ++ IN PGF + ++ +AR+KL+  LQ +
Sbjct: 204 FKIIVQIFMSGADDATMEALERSYTDLNYGMRAMAINLPGFAYYRALRARRKLVSVLQGV 263

Query: 253 INEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKLFAGHETTAYTAM 312
           ++ +R+   +  +   A D MD LI+ +DE G  L D+ I D++   L AGHE++ +  M
Sbjct: 264 LDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIVDVLIMYLNAGHESSGHITM 323

Query: 313 WAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQVIDETLRVGSITS 372
           WA +FL ++P  F +AK EQEE++R  P+TQ G+   +FK+M +LSQV+DETLR  +I+ 
Sbjct: 324 WATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQVVDETLRCVNISF 383

Query: 373 LLYRETTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSRWDNTRVKPGAFVP 432
           + +R+ T D+ +NG +IPKGWKV  W   +++++  +  P+ FNPSRW+    K G F+P
Sbjct: 384 VSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWEGPPPKAGTFLP 443

Query: 433 FGLGNRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPHPKDKCLARVFKV 489
           FGLG RLCPG+DLAKLEIS+FLHHFLL YK++R NPK ++ Y P P P D CLA + KV
Sbjct: 444 FGLGARLCPGNDLAKLEISVFLHHFLLGYKLKRANPKCRVRYLPHPRPVDNCLATITKV 502

BLAST of CsGy3G001750 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 6.1e-112
Identity = 199/474 (41.98%), Postives = 303/474 (63.92%), Query Frame = 0

Query: 17  LVLVFGVLKRLNDWYYEVKLGKL-WPKLPPGHMGWPFVGSTLSFIKDYTSGQPQNFVKAL 76
           LV+V    +R + WY E  LG     +LPPG MGWP VG   +F++ + SG+P  F+ + 
Sbjct: 45  LVVVDAAARRAHGWYREAALGAARRARLPPGEMGWPLVGGMWAFLRAFKSGKPDAFIASF 104

Query: 77  QIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPKNIVILSGRKSLMQVTK 136
             R+G+  +Y++ +   P+++V T E C+QVL +++ F    PK  V L G +S + +  
Sbjct: 105 VRRFGRTGVYRSFMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKATVALVGPRSFVAMPY 164

Query: 137 AEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPIELLTEMKKLTFKVIW 196
            EHRR+R+LT API+   AL  Y+  I++TV + L  WA     +E LTE++++TFK+I 
Sbjct: 165 DEHRRIRKLTAAPINGFDALTGYLPFIDRTVTSSLRAWADHGGSVEFLTELRRMTFKIIV 224

Query: 197 NIFMGSTSSMGENESSLFY-EVAAGFLSLPINFPGFGFRKSFKARQKLMERLQCIINEKR 256
            IF+G             Y E+  G  ++ IN PGF +R + +AR++L+  LQ +++E+R
Sbjct: 225 QIFLGGADQATTRALERSYTELNYGMRAMAINLPGFAYRGALRARRRLVAVLQGVLDERR 284

Query: 257 SMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKLFAGHETTAYTAMWAVLF 316
           + +  KG +    D MD LI+ +DE G  LDD+ I D++   L AGHE++ +  MWA +F
Sbjct: 285 AAR-AKGVSGGGVDMMDRLIEAQDERGRHLDDDEIIDVLVMYLNAGHESSGHITMWATVF 344

Query: 317 LTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQVIDETLRVGSITSLLYRE 376
           L ++P  F +AK EQE ++R  PS+Q+G+   +F++M+YLSQVIDETLR+ +I+ + +R+
Sbjct: 345 LQENPDMFARAKAEQEAIMRSIPSSQRGLTLRDFRKMEYLSQVIDETLRLVNISFVSFRQ 404

Query: 377 TTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSRWDNTRVKPGAFVPFGLGN 436
            T DV +NG +IPKGWKV  W   ++++   +  P  F+PSRW+    + G F+ FGLG 
Sbjct: 405 ATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPTKFDPSRWEGHSPRAGTFLAFGLGA 464

Query: 437 RLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPHPKDKCLARVFKV 489
           RLCPG+DLAKLEIS+FLHHFLL YK+ R NP+ ++ Y P P P D CLA++ +V
Sbjct: 465 RLCPGNDLAKLEISVFLHHFLLGYKLARTNPRCRVRYLPHPRPVDNCLAKITRV 517

BLAST of CsGy3G001750 vs. NCBI nr
Match: XP_004148755.3 (beta-amyrin 11-oxidase [Cucumis sativus] >KAE8650034.1 hypothetical protein Csa_011067 [Cucumis sativus])

HSP 1 Score: 990 bits (2560), Expect = 0.0
Identity = 489/489 (100.00%), Postives = 489/489 (100.00%), Query Frame = 0

Query: 1   MELKMMMNWLVLIFGGLVLVFGVLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSFI 60
           MELKMMMNWLVLIFGGLVLVFGVLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSFI
Sbjct: 1   MELKMMMNWLVLIFGGLVLVFGVLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSFI 60

Query: 61  KDYTSGQPQNFVKALQIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPKN 120
           KDYTSGQPQNFVKALQIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPKN
Sbjct: 61  KDYTSGQPQNFVKALQIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPKN 120

Query: 121 IVILSGRKSLMQVTKAEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPI 180
           IVILSGRKSLMQVTKAEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPI
Sbjct: 121 IVILSGRKSLMQVTKAEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPI 180

Query: 181 ELLTEMKKLTFKVIWNIFMGSTSSMGENESSLFYEVAAGFLSLPINFPGFGFRKSFKARQ 240
           ELLTEMKKLTFKVIWNIFMGSTSSMGENESSLFYEVAAGFLSLPINFPGFGFRKSFKARQ
Sbjct: 181 ELLTEMKKLTFKVIWNIFMGSTSSMGENESSLFYEVAAGFLSLPINFPGFGFRKSFKARQ 240

Query: 241 KLMERLQCIINEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKLFAG 300
           KLMERLQCIINEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKLFAG
Sbjct: 241 KLMERLQCIINEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKLFAG 300

Query: 301 HETTAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQVIDE 360
           HETTAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQVIDE
Sbjct: 301 HETTAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQVIDE 360

Query: 361 TLRVGSITSLLYRETTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSRWDNT 420
           TLRVGSITSLLYRETTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSRWDNT
Sbjct: 361 TLRVGSITSLLYRETTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSRWDNT 420

Query: 421 RVKPGAFVPFGLGNRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPHPKDK 480
           RVKPGAFVPFGLGNRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPHPKDK
Sbjct: 421 RVKPGAFVPFGLGNRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPHPKDK 480

Query: 481 CLARVFKVE 489
           CLARVFKVE
Sbjct: 481 CLARVFKVE 489

BLAST of CsGy3G001750 vs. NCBI nr
Match: XP_008448714.1 (PREDICTED: beta-amyrin 11-oxidase-like [Cucumis melo])

HSP 1 Score: 934 bits (2414), Expect = 0.0
Identity = 458/489 (93.66%), Postives = 477/489 (97.55%), Query Frame = 0

Query: 1   MELKMMMNWLVL-IFGGLVLVFGVLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSF 60
           MELKMMMNWLVL IFGGLVLVFGVLKRLNDWYYE KLGKLWPKLPPGHMGWPF+GS+LSF
Sbjct: 1   MELKMMMNWLVLLIFGGLVLVFGVLKRLNDWYYEAKLGKLWPKLPPGHMGWPFLGSSLSF 60

Query: 61  IKDYTSGQPQNFVKALQIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPK 120
           IKDYT+G+P++F+K LQIR+GKADMYKTHLLGR  +IVYTPEICRQVLTNEEIF PSLPK
Sbjct: 61  IKDYTAGRPRSFLKTLQIRHGKADMYKTHLLGRACVIVYTPEICRQVLTNEEIFIPSLPK 120

Query: 121 NIVILSGRKSLMQVTKAEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSP 180
           NIVILSGRKSLMQVTKAEHRRLRRLTTAP+SSHTALE+YINDIEQTVMNGLEEWASMKSP
Sbjct: 121 NIVILSGRKSLMQVTKAEHRRLRRLTTAPVSSHTALEMYINDIEQTVMNGLEEWASMKSP 180

Query: 181 IELLTEMKKLTFKVIWNIFMGSTSSMGENESSLFYEVAAGFLSLPINFPGFGFRKSFKAR 240
           IELL EMKKLTFKVIWNIFMGSTSSMGENESSLFYEVAAGFLSLPINFPGFGF KSFKAR
Sbjct: 181 IELLNEMKKLTFKVIWNIFMGSTSSMGENESSLFYEVAAGFLSLPINFPGFGFHKSFKAR 240

Query: 241 QKLMERLQCIINEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKLFA 300
           +KLMERLQCII+EKRSMK RKGENWEAKDTMD LIDVKDEDGEELDDETIRDLIFGKLFA
Sbjct: 241 EKLMERLQCIIDEKRSMKTRKGENWEAKDTMDLLIDVKDEDGEELDDETIRDLIFGKLFA 300

Query: 301 GHETTAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQVID 360
           GHET+AYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGIN SEFKQMKYLSQVID
Sbjct: 301 GHETSAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINLSEFKQMKYLSQVID 360

Query: 361 ETLRVGSITSLLYRETTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSRWDN 420
           ETLRVGSITSLLYRETTIDVEINGKIIPKGW+VLPWLSELY+NET+FSSPQDFNPSRWDN
Sbjct: 361 ETLRVGSITSLLYRETTIDVEINGKIIPKGWRVLPWLSELYMNETSFSSPQDFNPSRWDN 420

Query: 421 TRVKPGAFVPFGLGNRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPHPKD 480
            RVKPGAFVPFGLGNRLCPGSDLAKLEISIFLH+FLLNYKVERLNPKSKLTYAPFPHP+D
Sbjct: 421 VRVKPGAFVPFGLGNRLCPGSDLAKLEISIFLHYFLLNYKVERLNPKSKLTYAPFPHPED 480

Query: 481 KCLARVFKV 488
           KCLARVFKV
Sbjct: 481 KCLARVFKV 489

BLAST of CsGy3G001750 vs. NCBI nr
Match: KAA0053059.1 (beta-amyrin 11-oxidase-like [Cucumis melo var. makuwa] >TYK11514.1 beta-amyrin 11-oxidase-like [Cucumis melo var. makuwa])

HSP 1 Score: 927 bits (2395), Expect = 0.0
Identity = 454/485 (93.61%), Postives = 473/485 (97.53%), Query Frame = 0

Query: 5   MMMNWLVL-IFGGLVLVFGVLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSFIKDY 64
           MMMNWLVL IFGGLVLVFGVLKRLNDWYYE KLGKLWPKLPPGHMGWPF+GS+LSFIKDY
Sbjct: 1   MMMNWLVLLIFGGLVLVFGVLKRLNDWYYEAKLGKLWPKLPPGHMGWPFLGSSLSFIKDY 60

Query: 65  TSGQPQNFVKALQIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPKNIVI 124
           T+G+P++F+K LQIR+GKADMYKTHLLGR  +IVYTPEICRQVLTNEEIF PSLPKNIVI
Sbjct: 61  TAGRPRSFLKTLQIRHGKADMYKTHLLGRACVIVYTPEICRQVLTNEEIFIPSLPKNIVI 120

Query: 125 LSGRKSLMQVTKAEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPIELL 184
           LSGRKSLMQVTKAEHRRLRRLTTAP+SSHTALE+YINDIEQTVMNGLEEWASMKSPIELL
Sbjct: 121 LSGRKSLMQVTKAEHRRLRRLTTAPVSSHTALEMYINDIEQTVMNGLEEWASMKSPIELL 180

Query: 185 TEMKKLTFKVIWNIFMGSTSSMGENESSLFYEVAAGFLSLPINFPGFGFRKSFKARQKLM 244
            EMKKLTFKVIWNIFMGSTSSMGENESSLFYEVAAGFLSLPINFPGFGF KSFKAR+KLM
Sbjct: 181 NEMKKLTFKVIWNIFMGSTSSMGENESSLFYEVAAGFLSLPINFPGFGFHKSFKAREKLM 240

Query: 245 ERLQCIINEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKLFAGHET 304
           ERLQCII+EKRSMK RKGENWEAKDTMD LIDVKDEDGEELDDETIRDLIFGKLFAGHET
Sbjct: 241 ERLQCIIDEKRSMKTRKGENWEAKDTMDLLIDVKDEDGEELDDETIRDLIFGKLFAGHET 300

Query: 305 TAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQVIDETLR 364
           +AYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGIN SEFKQMKYLSQVIDETLR
Sbjct: 301 SAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINLSEFKQMKYLSQVIDETLR 360

Query: 365 VGSITSLLYRETTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSRWDNTRVK 424
           VGSITSLLYRETTIDVEINGKIIPKGW+VLPWLSELY+NET+FSSPQDFNPSRWDN RVK
Sbjct: 361 VGSITSLLYRETTIDVEINGKIIPKGWRVLPWLSELYMNETSFSSPQDFNPSRWDNVRVK 420

Query: 425 PGAFVPFGLGNRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPHPKDKCLA 484
           PGAFVPFGLGNRLCPGSDLAKLEISIFLH+FLLNYKVERLNPKSKLTYAPFPHP+DKCLA
Sbjct: 421 PGAFVPFGLGNRLCPGSDLAKLEISIFLHYFLLNYKVERLNPKSKLTYAPFPHPEDKCLA 480

Query: 485 RVFKV 488
           RVFKV
Sbjct: 481 RVFKV 485

BLAST of CsGy3G001750 vs. NCBI nr
Match: XP_008448712.1 (PREDICTED: beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo])

HSP 1 Score: 736 bits (1899), Expect = 1.07e-263
Identity = 359/492 (72.97%), Postives = 419/492 (85.16%), Query Frame = 0

Query: 1   MELKMMMNWLVLIFGGLVLVFGVLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSFI 60
           MELK    WLVLI G L L+FGV+KRLN WYYE KLGKLWPKLPPG MGWP +GSTLSF+
Sbjct: 1   MELK---EWLVLIIGSLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGSTLSFL 60

Query: 61  KDYTSGQPQNFVKALQIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPKN 120
           K++ SGQP+  +  L IRYGK+DMYKTHLLGR SIIV T E+CRQVL +EE F PSLP++
Sbjct: 61  KNFNSGQPRILLHNLSIRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRS 120

Query: 121 IVILSGRKSLMQVTKAEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPI 180
           I +LSGRKSLMQV KAEHRRLRRLT APIS H+ALE+YIN IEQTV++GLEEWASMK PI
Sbjct: 121 ITLLSGRKSLMQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMKKPI 180

Query: 181 ELLTEMKKLTFKVIWNIFMGSTS---SMGENESSLFYEVAAGFLSLPINFPGFGFRKSFK 240
           ELLTE+KKLTFK+IWNIFMGS+S   S+GE E+ LF ++A GF+SLPINFPGF F KS K
Sbjct: 181 ELLTEIKKLTFKIIWNIFMGSSSIDSSIGEIEA-LFAQIALGFISLPINFPGFSFHKSLK 240

Query: 241 ARQKLMERLQCIINEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKL 300
           AR++L+  LQ IIN+KR  K+++GENWEAKD MD LI++KDEDGEELDDETIRDLIFGKL
Sbjct: 241 ARKELLSLLQSIINQKRMEKKKEGENWEAKDMMDLLIELKDEDGEELDDETIRDLIFGKL 300

Query: 301 FAGHETTAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQV 360
           FAGHET+AYTAMWAVLFLT+HPH  Q+ KEEQE++++RRP+TQKG++ SE KQMK+L QV
Sbjct: 301 FAGHETSAYTAMWAVLFLTNHPHILQRTKEEQEDIVKRRPTTQKGLSLSEVKQMKFLYQV 360

Query: 361 IDETLRVGSITSLLYRETTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSRW 420
           IDETLRVGSIT +++RE  +DVEINGKIIPKGWKV+PW  ELY++E  F SP +FNPSRW
Sbjct: 361 IDETLRVGSITFMVFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKQFPSPHEFNPSRW 420

Query: 421 DNTRVKPGAFVPFGLGNRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPHP 480
           DN    PGAF PFGLG+R CPGSDLAKLEISIFLH+FLLNYKVE+L+PK + T  PFPHP
Sbjct: 421 DNFVATPGAFTPFGLGSRYCPGSDLAKLEISIFLHYFLLNYKVEQLDPKCQKTCLPFPHP 480

Query: 481 KDKCLARVFKVE 489
           KDK LARV K+E
Sbjct: 481 KDKGLARVHKLE 488

BLAST of CsGy3G001750 vs. NCBI nr
Match: XP_023552542.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 729 bits (1883), Expect = 3.92e-261
Identity = 352/492 (71.54%), Postives = 415/492 (84.35%), Query Frame = 0

Query: 1   MELKMMMNWLVLIFGGLVLVFGVLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSFI 60
           ME+K   NWLVLI    V V G+LKR+N WYY  KLGK WPKLPPGHMGWP +GST+S+I
Sbjct: 4   MEVK---NWLVLIVAAFVFVLGILKRVNGWYYAAKLGKTWPKLPPGHMGWPLLGSTISYI 63

Query: 61  KDYTSGQPQNFVKALQIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPKN 120
           K++ SGQP NF+    IRYGK+DMYKTH+LGR +IIV + E+CRQVL +E+ F PSLPKN
Sbjct: 64  KNFISGQPSNFMANFYIRYGKSDMYKTHILGRATIIVCSSELCRQVLGDEDNFKPSLPKN 123

Query: 121 IVILSGRKSLMQVTKAEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPI 180
           I +L+GRKSL+QV KAEH+RLRRLT APIS H ALE+YIN IE TVM+GLEEWASMK+PI
Sbjct: 124 INMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPI 183

Query: 181 ELLTEMKKLTFKVIWNIFMGSTS---SMGENESSLFYEVAAGFLSLPINFPGFGFRKSFK 240
           ELL+E+KKLTFK+IWNIFMGS+S   S+GE E  LF EVA GF+SLPINFPGF   K+ K
Sbjct: 184 ELLSEIKKLTFKIIWNIFMGSSSLESSIGEMEG-LFSEVALGFISLPINFPGFNLHKALK 243

Query: 241 ARQKLMERLQCIINEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKL 300
           AR+KL++ LQ IIN+KR ++ER+G +WEAKD MD L++VKDEDGEELDDETIRDLIFGKL
Sbjct: 244 ARKKLLDILQSIINQKRLVRERQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKL 303

Query: 301 FAGHETTAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQV 360
           FAGHET+AYTAMWAVLFLT HPH FQ+AKEEQ+ +I +RP+TQKGI  SE KQMK+LSQ+
Sbjct: 304 FAGHETSAYTAMWAVLFLTKHPHIFQRAKEEQDRIITQRPTTQKGIILSEVKQMKFLSQI 363

Query: 361 IDETLRVGSITSLLYRETTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSRW 420
           IDETLRVG +T +L+RE  +DVEINGKIIPKGWKV+PW  ELY++E  F SPQ+FNPSRW
Sbjct: 364 IDETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPQEFNPSRW 423

Query: 421 DNTRVKPGAFVPFGLGNRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPHP 480
           DN    PGAF+PFG G+R CPGSDLAKLEISIFLH+FLLNYK+E+LNPK  +TY PFPHP
Sbjct: 424 DNYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHP 483

Query: 481 KDKCLARVFKVE 489
           KDKCLARV KV+
Sbjct: 484 KDKCLARVHKVK 491

BLAST of CsGy3G001750 vs. ExPASy TrEMBL
Match: A0A1S3BKC3 (beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103490802 PE=3 SV=1)

HSP 1 Score: 934 bits (2414), Expect = 0.0
Identity = 458/489 (93.66%), Postives = 477/489 (97.55%), Query Frame = 0

Query: 1   MELKMMMNWLVL-IFGGLVLVFGVLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSF 60
           MELKMMMNWLVL IFGGLVLVFGVLKRLNDWYYE KLGKLWPKLPPGHMGWPF+GS+LSF
Sbjct: 1   MELKMMMNWLVLLIFGGLVLVFGVLKRLNDWYYEAKLGKLWPKLPPGHMGWPFLGSSLSF 60

Query: 61  IKDYTSGQPQNFVKALQIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPK 120
           IKDYT+G+P++F+K LQIR+GKADMYKTHLLGR  +IVYTPEICRQVLTNEEIF PSLPK
Sbjct: 61  IKDYTAGRPRSFLKTLQIRHGKADMYKTHLLGRACVIVYTPEICRQVLTNEEIFIPSLPK 120

Query: 121 NIVILSGRKSLMQVTKAEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSP 180
           NIVILSGRKSLMQVTKAEHRRLRRLTTAP+SSHTALE+YINDIEQTVMNGLEEWASMKSP
Sbjct: 121 NIVILSGRKSLMQVTKAEHRRLRRLTTAPVSSHTALEMYINDIEQTVMNGLEEWASMKSP 180

Query: 181 IELLTEMKKLTFKVIWNIFMGSTSSMGENESSLFYEVAAGFLSLPINFPGFGFRKSFKAR 240
           IELL EMKKLTFKVIWNIFMGSTSSMGENESSLFYEVAAGFLSLPINFPGFGF KSFKAR
Sbjct: 181 IELLNEMKKLTFKVIWNIFMGSTSSMGENESSLFYEVAAGFLSLPINFPGFGFHKSFKAR 240

Query: 241 QKLMERLQCIINEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKLFA 300
           +KLMERLQCII+EKRSMK RKGENWEAKDTMD LIDVKDEDGEELDDETIRDLIFGKLFA
Sbjct: 241 EKLMERLQCIIDEKRSMKTRKGENWEAKDTMDLLIDVKDEDGEELDDETIRDLIFGKLFA 300

Query: 301 GHETTAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQVID 360
           GHET+AYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGIN SEFKQMKYLSQVID
Sbjct: 301 GHETSAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINLSEFKQMKYLSQVID 360

Query: 361 ETLRVGSITSLLYRETTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSRWDN 420
           ETLRVGSITSLLYRETTIDVEINGKIIPKGW+VLPWLSELY+NET+FSSPQDFNPSRWDN
Sbjct: 361 ETLRVGSITSLLYRETTIDVEINGKIIPKGWRVLPWLSELYMNETSFSSPQDFNPSRWDN 420

Query: 421 TRVKPGAFVPFGLGNRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPHPKD 480
            RVKPGAFVPFGLGNRLCPGSDLAKLEISIFLH+FLLNYKVERLNPKSKLTYAPFPHP+D
Sbjct: 421 VRVKPGAFVPFGLGNRLCPGSDLAKLEISIFLHYFLLNYKVERLNPKSKLTYAPFPHPED 480

Query: 481 KCLARVFKV 488
           KCLARVFKV
Sbjct: 481 KCLARVFKV 489

BLAST of CsGy3G001750 vs. ExPASy TrEMBL
Match: A0A5A7UHW0 (Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G001780 PE=3 SV=1)

HSP 1 Score: 927 bits (2395), Expect = 0.0
Identity = 454/485 (93.61%), Postives = 473/485 (97.53%), Query Frame = 0

Query: 5   MMMNWLVL-IFGGLVLVFGVLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSFIKDY 64
           MMMNWLVL IFGGLVLVFGVLKRLNDWYYE KLGKLWPKLPPGHMGWPF+GS+LSFIKDY
Sbjct: 1   MMMNWLVLLIFGGLVLVFGVLKRLNDWYYEAKLGKLWPKLPPGHMGWPFLGSSLSFIKDY 60

Query: 65  TSGQPQNFVKALQIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPKNIVI 124
           T+G+P++F+K LQIR+GKADMYKTHLLGR  +IVYTPEICRQVLTNEEIF PSLPKNIVI
Sbjct: 61  TAGRPRSFLKTLQIRHGKADMYKTHLLGRACVIVYTPEICRQVLTNEEIFIPSLPKNIVI 120

Query: 125 LSGRKSLMQVTKAEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPIELL 184
           LSGRKSLMQVTKAEHRRLRRLTTAP+SSHTALE+YINDIEQTVMNGLEEWASMKSPIELL
Sbjct: 121 LSGRKSLMQVTKAEHRRLRRLTTAPVSSHTALEMYINDIEQTVMNGLEEWASMKSPIELL 180

Query: 185 TEMKKLTFKVIWNIFMGSTSSMGENESSLFYEVAAGFLSLPINFPGFGFRKSFKARQKLM 244
            EMKKLTFKVIWNIFMGSTSSMGENESSLFYEVAAGFLSLPINFPGFGF KSFKAR+KLM
Sbjct: 181 NEMKKLTFKVIWNIFMGSTSSMGENESSLFYEVAAGFLSLPINFPGFGFHKSFKAREKLM 240

Query: 245 ERLQCIINEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKLFAGHET 304
           ERLQCII+EKRSMK RKGENWEAKDTMD LIDVKDEDGEELDDETIRDLIFGKLFAGHET
Sbjct: 241 ERLQCIIDEKRSMKTRKGENWEAKDTMDLLIDVKDEDGEELDDETIRDLIFGKLFAGHET 300

Query: 305 TAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQVIDETLR 364
           +AYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGIN SEFKQMKYLSQVIDETLR
Sbjct: 301 SAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINLSEFKQMKYLSQVIDETLR 360

Query: 365 VGSITSLLYRETTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSRWDNTRVK 424
           VGSITSLLYRETTIDVEINGKIIPKGW+VLPWLSELY+NET+FSSPQDFNPSRWDN RVK
Sbjct: 361 VGSITSLLYRETTIDVEINGKIIPKGWRVLPWLSELYMNETSFSSPQDFNPSRWDNVRVK 420

Query: 425 PGAFVPFGLGNRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPHPKDKCLA 484
           PGAFVPFGLGNRLCPGSDLAKLEISIFLH+FLLNYKVERLNPKSKLTYAPFPHP+DKCLA
Sbjct: 421 PGAFVPFGLGNRLCPGSDLAKLEISIFLHYFLLNYKVERLNPKSKLTYAPFPHPEDKCLA 480

Query: 485 RVFKV 488
           RVFKV
Sbjct: 481 RVFKV 485

BLAST of CsGy3G001750 vs. ExPASy TrEMBL
Match: A0A0A0L415 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G011850 PE=3 SV=1)

HSP 1 Score: 858 bits (2216), Expect = 4.41e-313
Identity = 439/489 (89.78%), Postives = 439/489 (89.78%), Query Frame = 0

Query: 1   MELKMMMNWLVLIFGGLVLVFGVLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSFI 60
           MELKMMMNWLVLIFGGLVLVFGVLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSFI
Sbjct: 1   MELKMMMNWLVLIFGGLVLVFGVLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSFI 60

Query: 61  KDYTSGQPQNFVKALQIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPKN 120
           KDYTSGQPQNFVKALQIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPKN
Sbjct: 61  KDYTSGQPQNFVKALQIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPKN 120

Query: 121 IVILSGRKSLMQVTKAEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPI 180
           IVILSGRKSLMQVTKAEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPI
Sbjct: 121 IVILSGRKSLMQVTKAEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPI 180

Query: 181 ELLTEMKKLTFKVIWNIFMGSTSSMGENESSLFYEVAAGFLSLPINFPGFGFRKSFKARQ 240
           ELLTEMKKLTFKVIWNIFMGSTSSMGENESSLFYEVAAGFLSLPINFPGFGFRKSFKARQ
Sbjct: 181 ELLTEMKKLTFKVIWNIFMGSTSSMGENESSLFYEVAAGFLSLPINFPGFGFRKSFKARQ 240

Query: 241 KLMERLQCIINEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKLFAG 300
           KLMERLQCIINEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKLFAG
Sbjct: 241 KLMERLQCIINEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKLFAG 300

Query: 301 HETTAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQVIDE 360
           HETTAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQVIDE
Sbjct: 301 HETTAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQVIDE 360

Query: 361 TLRVGSITSLLYRETTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSRWDNT 420
           TLRVGSITSLLYRETTIDVEING                                     
Sbjct: 361 TLRVGSITSLLYRETTIDVEING------------------------------------- 420

Query: 421 RVKPGAFVPFGLGNRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPHPKDK 480
                        NRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPHPKDK
Sbjct: 421 -------------NRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPHPKDK 439

Query: 481 CLARVFKVE 489
           CLARVFKVE
Sbjct: 481 CLARVFKVE 439

BLAST of CsGy3G001750 vs. ExPASy TrEMBL
Match: A0A1S3BJR1 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490801 PE=3 SV=1)

HSP 1 Score: 736 bits (1899), Expect = 5.20e-264
Identity = 359/492 (72.97%), Postives = 419/492 (85.16%), Query Frame = 0

Query: 1   MELKMMMNWLVLIFGGLVLVFGVLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSFI 60
           MELK    WLVLI G L L+FGV+KRLN WYYE KLGKLWPKLPPG MGWP +GSTLSF+
Sbjct: 1   MELK---EWLVLIIGSLALIFGVMKRLNGWYYEAKLGKLWPKLPPGDMGWPILGSTLSFL 60

Query: 61  KDYTSGQPQNFVKALQIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPKN 120
           K++ SGQP+  +  L IRYGK+DMYKTHLLGR SIIV T E+CRQVL +EE F PSLP++
Sbjct: 61  KNFNSGQPRILLHNLSIRYGKSDMYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRS 120

Query: 121 IVILSGRKSLMQVTKAEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPI 180
           I +LSGRKSLMQV KAEHRRLRRLT APIS H+ALE+YIN IEQTV++GLEEWASMK PI
Sbjct: 121 ITLLSGRKSLMQVFKAEHRRLRRLTMAPISGHSALEMYINHIEQTVISGLEEWASMKKPI 180

Query: 181 ELLTEMKKLTFKVIWNIFMGSTS---SMGENESSLFYEVAAGFLSLPINFPGFGFRKSFK 240
           ELLTE+KKLTFK+IWNIFMGS+S   S+GE E+ LF ++A GF+SLPINFPGF F KS K
Sbjct: 181 ELLTEIKKLTFKIIWNIFMGSSSIDSSIGEIEA-LFAQIALGFISLPINFPGFSFHKSLK 240

Query: 241 ARQKLMERLQCIINEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKL 300
           AR++L+  LQ IIN+KR  K+++GENWEAKD MD LI++KDEDGEELDDETIRDLIFGKL
Sbjct: 241 ARKELLSLLQSIINQKRMEKKKEGENWEAKDMMDLLIELKDEDGEELDDETIRDLIFGKL 300

Query: 301 FAGHETTAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQV 360
           FAGHET+AYTAMWAVLFLT+HPH  Q+ KEEQE++++RRP+TQKG++ SE KQMK+L QV
Sbjct: 301 FAGHETSAYTAMWAVLFLTNHPHILQRTKEEQEDIVKRRPTTQKGLSLSEVKQMKFLYQV 360

Query: 361 IDETLRVGSITSLLYRETTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSRW 420
           IDETLRVGSIT +++RE  +DVEINGKIIPKGWKV+PW  ELY++E  F SP +FNPSRW
Sbjct: 361 IDETLRVGSITFMVFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKQFPSPHEFNPSRW 420

Query: 421 DNTRVKPGAFVPFGLGNRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPHP 480
           DN    PGAF PFGLG+R CPGSDLAKLEISIFLH+FLLNYKVE+L+PK + T  PFPHP
Sbjct: 421 DNFVATPGAFTPFGLGSRYCPGSDLAKLEISIFLHYFLLNYKVEQLDPKCQKTCLPFPHP 480

Query: 481 KDKCLARVFKVE 489
           KDK LARV K+E
Sbjct: 481 KDKGLARVHKLE 488

BLAST of CsGy3G001750 vs. ExPASy TrEMBL
Match: A0A6J1L1N7 (beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111500298 PE=3 SV=1)

HSP 1 Score: 720 bits (1859), Expect = 6.82e-258
Identity = 349/491 (71.08%), Postives = 412/491 (83.91%), Query Frame = 0

Query: 1   MELKMMMNWLVLIFGGLVLVFGVLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSFI 60
           ME+K   NWLVLI    V V G+LKRLN WYY  KLGK WPKLPPG MGWP +GST+S+I
Sbjct: 4   MEVK---NWLVLIVAAFVFVLGILKRLNGWYYAAKLGKTWPKLPPGDMGWPLLGSTISYI 63

Query: 61  KDYTSGQPQNFVKALQIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPKN 120
           K++ SGQP NF+    IRYGK DMYKTHLLGR +IIV + E+CRQVL +E+ F PSLPKN
Sbjct: 64  KNFISGQPSNFIANFYIRYGKRDMYKTHLLGRATIIVCSSELCRQVLGDEDNFKPSLPKN 123

Query: 121 IVILSGRKSLMQVTKAEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPI 180
           I +L+GRKSL+QV KAEH+RLRRLT APIS H ALE+YIN IE TVM+GLEEWASMK+PI
Sbjct: 124 INMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPI 183

Query: 181 ELLTEMKKLTFKVIWNIFMGSTS---SMGENESSLFYEVAAGFLSLPINFPGFGFRKSFK 240
           ELL+E+KKLTFK+IWNIFMGS+S   S+GE E  LF EVA GF+SLPINFPGF   K+ K
Sbjct: 184 ELLSEIKKLTFKIIWNIFMGSSSLESSIGEMEG-LFSEVALGFISLPINFPGFNLHKALK 243

Query: 241 ARQKLMERLQCIINEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKL 300
           AR+KL++ LQ II++KR +++R+G +WEAKD MD L++VKDEDGEELDDETIRDLIFGKL
Sbjct: 244 ARKKLLDILQSIIDQKRLVRKRQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKL 303

Query: 301 FAGHETTAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQV 360
           FAGHET+AYTAMWAVLFLT HPH FQ+AKEEQE ++ +RP+TQKGI  SE K+MK+LSQ+
Sbjct: 304 FAGHETSAYTAMWAVLFLTKHPHIFQRAKEEQELIVAQRPTTQKGIILSEVKRMKFLSQI 363

Query: 361 IDETLRVGSITSLLYRETTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSRW 420
           IDETLRVG +T +L+RE  +DVEINGKIIPKGWKV+PW  ELY++E  F SP++FNPSRW
Sbjct: 364 IDETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPREFNPSRW 423

Query: 421 DNTRVKPGAFVPFGLGNRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPHP 480
           DN    PGAF+PFG G+R CPGSDLAKLEISIFLH+FLLNYK+E+LNPK  +TY PFPHP
Sbjct: 424 DNYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHP 483

Query: 481 KDKCLARVFKV 488
           KDKCLARV KV
Sbjct: 484 KDKCLARVHKV 490

BLAST of CsGy3G001750 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 437.2 bits (1123), Expect = 1.8e-122
Identity = 217/483 (44.93%), Postives = 319/483 (66.05%), Query Frame = 0

Query: 9   WLVLIFGGLVLVFGVLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSFIKDYTSGQP 68
           W  L+  G   +  +++++N W YE  LG+    LPPG +GWPF+G+ LSF++ + +  P
Sbjct: 11  WFPLMVLGCFGLNWLVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDP 70

Query: 69  QNFVKALQIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPKNIVILSGRK 128
            +F + L  RYG   +YK H+ G PSIIV T + CR+VLT+++ F P  P + + L GRK
Sbjct: 71  DSFTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRK 130

Query: 129 SLMQVTKAEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPIELLTEMKK 188
           S + ++  EH+RLRRLT AP++ H AL  YI  IE+ V+  L++W  M    E LT ++K
Sbjct: 131 SFVGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKM-GEFEFLTHLRK 190

Query: 189 LTFKVIWNIFMGSTS-----SMGENESSLFYEVAAGFLSLPINFPGFGFRKSFKARQKLM 248
           LTF++I  IF+ S S     ++    ++L Y V A    + +N PGF + ++ KAR+ L+
Sbjct: 191 LTFRIIMYIFLSSESENVMDALEREYTALNYGVRA----MAVNIPGFAYHRALKARKTLV 250

Query: 249 ERLQCIINEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKLFAGHET 308
              Q I+ E+R+ +++   +   KD +D L++VKDEDG+ LDDE I D++   L AGHE+
Sbjct: 251 AAFQSIVTERRNQRKQNILS-NKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHES 310

Query: 309 TAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQVIDETLR 368
           + +T MWA +FL +HP   Q+AK EQE +++ RP  QKG++  E ++M++LSQV+DETLR
Sbjct: 311 SGHTIMWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLR 370

Query: 369 VGSITSLLYRETTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSRWDNTRV- 428
           V + +   +RE   DVE+NG +IPKGWKVL W  +++++   F  P+ F+P+RWDN  V 
Sbjct: 371 VITFSLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVP 430

Query: 429 KPGAFVPFGLGNRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPHPKDKCL 486
           K GAF+PFG G+ LCPG+DLAKLEISIFLHHFLL Y+V+R NP+  + Y P   P D CL
Sbjct: 431 KAGAFLPFGAGSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCL 487

BLAST of CsGy3G001750 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 437.2 bits (1123), Expect = 1.8e-122
Identity = 223/489 (45.60%), Postives = 321/489 (65.64%), Query Frame = 0

Query: 2   ELKMMMNWLVLIFGGLVLVFGVLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSFIK 61
           E  +++ W  LI  GL ++  VLKR+N W Y  KLG+    LPPG +GWP +G+  SF++
Sbjct: 3   ETGLILMWFPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLR 62

Query: 62  DYTSGQPQNFVKALQIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPKNI 121
            + +  P++F+++   RYG+  +YK H+ G P ++V TPE CR+VLT+++ F    PK+ 
Sbjct: 63  AFKTSDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKST 122

Query: 122 VILSGRKSLMQVTKAEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPIE 181
           + L GRKS + ++  EH+RLRRLT+AP++   AL +YI  IE+TV   LE+W+ M   IE
Sbjct: 123 MKLIGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM-GEIE 182

Query: 182 LLTEMKKLTFKVIWNIFMGSTS-----SMGENESSLFYEVAAGFLSLPINFPGFGFRKSF 241
            L+ ++KLTFKVI  IF+ S S     S+    ++L Y V A    + IN PGF + ++ 
Sbjct: 183 FLSHLRKLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVRA----MGINLPGFAYHRAL 242

Query: 242 KARQKLMERLQCIINEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGK 301
           KAR+KL+   Q I+  +R+ +++   +   KD +D LIDVKDE+G  LDDE I DL+   
Sbjct: 243 KARKKLVAAFQSIVTNRRNQRKQNISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMY 302

Query: 302 LFAGHETTAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQ 361
           L AGHE++ +  MWA + + +HP   QKAKEEQE ++++R   QK +   E ++M YLSQ
Sbjct: 303 LNAGHESSGHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQ 362

Query: 362 VIDETLRVGSITSLLYRETTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSR 421
           VIDETLRV + +   +RE   DV+++G IIPKGWKVL W   ++++   +  P+ F+PSR
Sbjct: 363 VIDETLRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSR 422

Query: 422 WDNTRVKPGAFVPFGLGNRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPH 481
           W+    K G F+PFGLG+ LCPG+DLAKLEISIFLHHFLL Y+VER NP   + + P   
Sbjct: 423 WEGYTPKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNR 482

Query: 482 PKDKCLARV 486
           PKD CLAR+
Sbjct: 483 PKDNCLARI 484

BLAST of CsGy3G001750 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 437.2 bits (1123), Expect = 1.8e-122
Identity = 223/489 (45.60%), Postives = 321/489 (65.64%), Query Frame = 0

Query: 2   ELKMMMNWLVLIFGGLVLVFGVLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSFIK 61
           E  +++ W  LI  GL ++  VLKR+N W Y  KLG+    LPPG +GWP +G+  SF++
Sbjct: 3   ETGLILMWFPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLR 62

Query: 62  DYTSGQPQNFVKALQIRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPKNI 121
            + +  P++F+++   RYG+  +YK H+ G P ++V TPE CR+VLT+++ F    PK+ 
Sbjct: 63  AFKTSDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKST 122

Query: 122 VILSGRKSLMQVTKAEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPIE 181
           + L GRKS + ++  EH+RLRRLT+AP++   AL +YI  IE+TV   LE+W+ M   IE
Sbjct: 123 MKLIGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM-GEIE 182

Query: 182 LLTEMKKLTFKVIWNIFMGSTS-----SMGENESSLFYEVAAGFLSLPINFPGFGFRKSF 241
            L+ ++KLTFKVI  IF+ S S     S+    ++L Y V A    + IN PGF + ++ 
Sbjct: 183 FLSHLRKLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVRA----MGINLPGFAYHRAL 242

Query: 242 KARQKLMERLQCIINEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGK 301
           KAR+KL+   Q I+  +R+ +++   +   KD +D LIDVKDE+G  LDDE I DL+   
Sbjct: 243 KARKKLVAAFQSIVTNRRNQRKQNISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMY 302

Query: 302 LFAGHETTAYTAMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQ 361
           L AGHE++ +  MWA + + +HP   QKAKEEQE ++++R   QK +   E ++M YLSQ
Sbjct: 303 LNAGHESSGHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQ 362

Query: 362 VIDETLRVGSITSLLYRETTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSR 421
           VIDETLRV + +   +RE   DV+++G IIPKGWKVL W   ++++   +  P+ F+PSR
Sbjct: 363 VIDETLRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSR 422

Query: 422 WDNTRVKPGAFVPFGLGNRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPH 481
           W+    K G F+PFGLG+ LCPG+DLAKLEISIFLHHFLL Y+VER NP   + + P   
Sbjct: 423 WEGYTPKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNR 482

Query: 482 PKDKCLARV 486
           PKD CLAR+
Sbjct: 483 PKDNCLARI 484

BLAST of CsGy3G001750 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 256.5 bits (654), Expect = 4.2e-68
Identity = 163/478 (34.10%), Postives = 249/478 (52.09%), Query Frame = 0

Query: 17  LVLVFGVLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSFIKDYTSGQPQNFVKALQ 76
           L +V  V+  L  W+   K  +L  +LPPG MG P++G TL     YT   P +F    Q
Sbjct: 25  LTIVVVVVVLLFKWWLHWKEQRL--RLPPGSMGLPYIGETLRL---YTE-NPNSFFATRQ 84

Query: 77  IRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNE-EIFTPSLPKNIVILSGRKSLMQVTK 136
            +YG  D++KTH+LG P +++ +PE  R VL ++  +F P+ P +   + G ++L     
Sbjct: 85  NKYG--DIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQG 144

Query: 137 AEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPIELLTEMKKLTFKVIW 196
             H  L+RL  +     +AL   ++ IE  V+  L  W S KS I  L  MK+  F V  
Sbjct: 145 PYHSTLKRLVQSSFMP-SALRPTVSHIELLVLQTLSSWTSQKS-INTLEYMKRYAFDV-- 204

Query: 197 NIFMGSTSSMGENESS--------LFYEVAAGFLSLPINFPGFGFRKSFKARQKLMERLQ 256
                  S+ G+ E          L+  +  G+ S+P++ PG  F KS KAR +L E L+
Sbjct: 205 ----AIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELR 264

Query: 257 CIINEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKLFAGHETTAYT 316
            +I ++R     +G        +  L+  KD+    L D  I D I G +FA  +TTA  
Sbjct: 265 KVIEKRRENGREEG------GLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASV 324

Query: 317 AMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQVIDETLRVGSI 376
             W + +L DHP+  Q+   EQ  + ++     + I++ + ++M   ++VI ETLR  S+
Sbjct: 325 LTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASV 384

Query: 377 TSLLYRETTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSRWDNTRVKPGAF 436
            S  +RE   DVE +G +IPKGWKVLP    ++ +   F  P+ F+PSR++    KP  +
Sbjct: 385 LSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFE-VAPKPYTY 444

Query: 437 VPFGLGNRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPHPKDKCLARV 486
           +PFG G   CPGS+LAKLE+ I LHH   +++ E +  +  + Y PFP PK     RV
Sbjct: 445 MPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRV 479

BLAST of CsGy3G001750 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 256.5 bits (654), Expect = 4.2e-68
Identity = 163/478 (34.10%), Postives = 249/478 (52.09%), Query Frame = 0

Query: 17  LVLVFGVLKRLNDWYYEVKLGKLWPKLPPGHMGWPFVGSTLSFIKDYTSGQPQNFVKALQ 76
           L +V  V+  L  W+   K  +L  +LPPG MG P++G TL     YT   P +F    Q
Sbjct: 25  LTIVVVVVVLLFKWWLHWKEQRL--RLPPGSMGLPYIGETLRL---YTE-NPNSFFATRQ 84

Query: 77  IRYGKADMYKTHLLGRPSIIVYTPEICRQVLTNE-EIFTPSLPKNIVILSGRKSLMQVTK 136
            +YG  D++KTH+LG P +++ +PE  R VL ++  +F P+ P +   + G ++L     
Sbjct: 85  NKYG--DIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQG 144

Query: 137 AEHRRLRRLTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPIELLTEMKKLTFKVIW 196
             H  L+RL  +     +AL   ++ IE  V+  L  W S KS I  L  MK+  F V  
Sbjct: 145 PYHSTLKRLVQSSFMP-SALRPTVSHIELLVLQTLSSWTSQKS-INTLEYMKRYAFDV-- 204

Query: 197 NIFMGSTSSMGENESS--------LFYEVAAGFLSLPINFPGFGFRKSFKARQKLMERLQ 256
                  S+ G+ E          L+  +  G+ S+P++ PG  F KS KAR +L E L+
Sbjct: 205 ----AIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELR 264

Query: 257 CIINEKRSMKERKGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKLFAGHETTAYT 316
            +I ++R     +G        +  L+  KD+    L D  I D I G +FA  +TTA  
Sbjct: 265 KVIEKRRENGREEG------GLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASV 324

Query: 317 AMWAVLFLTDHPHTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQVIDETLRVGSI 376
             W + +L DHP+  Q+   EQ  + ++     + I++ + ++M   ++VI ETLR  S+
Sbjct: 325 LTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASV 384

Query: 377 TSLLYRETTIDVEINGKIIPKGWKVLPWLSELYVNETTFSSPQDFNPSRWDNTRVKPGAF 436
            S  +RE   DVE +G +IPKGWKVLP    ++ +   F  P+ F+PSR++    KP  +
Sbjct: 385 LSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFE-VAPKPYTY 444

Query: 437 VPFGLGNRLCPGSDLAKLEISIFLHHFLLNYKVERLNPKSKLTYAPFPHPKDKCLARV 486
           +PFG G   CPGS+LAKLE+ I LHH   +++ E +  +  + Y PFP PK     RV
Sbjct: 445 MPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRV 479

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B5BSX11.3e-13850.00Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1[more]
O230512.5e-12144.93Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1[more]
Q9C5Y22.5e-12145.60Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2[more]
Q5VRM71.8e-11643.22Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ... [more]
Q432466.1e-11241.98Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_004148755.30.0100.00beta-amyrin 11-oxidase [Cucumis sativus] >KAE8650034.1 hypothetical protein Csa_... [more]
XP_008448714.10.093.66PREDICTED: beta-amyrin 11-oxidase-like [Cucumis melo][more]
KAA0053059.10.093.61beta-amyrin 11-oxidase-like [Cucumis melo var. makuwa] >TYK11514.1 beta-amyrin 1... [more]
XP_008448712.11.07e-26372.97PREDICTED: beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo][more]
XP_023552542.13.92e-26171.54beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A1S3BKC30.093.66beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103490802 PE=3 SV=1[more]
A0A5A7UHW00.093.61Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A0A0L4154.41e-31389.78Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G011850 PE=3 SV=1[more]
A0A1S3BJR15.20e-26472.97beta-amyrin 11-oxidase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490801 P... [more]
A0A6J1L1N76.82e-25871.08beta-amyrin 11-oxidase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1115002... [more]
Match NameE-valueIdentityDescription
AT1G05160.11.8e-12244.93cytochrome P450, family 88, subfamily A, polypeptide 3 [more]
AT2G32440.11.8e-12245.60ent-kaurenoic acid hydroxylase 2 [more]
AT2G32440.21.8e-12245.60ent-kaurenoic acid hydroxylase 2 [more]
AT2G29090.14.2e-6834.10cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
AT2G29090.24.2e-6834.10cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 239..259
NoneNo IPR availablePANTHERPTHR24286:SF199CYTOCHROME P450 88D6coord: 21..466
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 21..466
IPR001128Cytochrome P450PRINTSPR00385P450coord: 428..437
score: 54.72
coord: 437..448
score: 39.48
coord: 299..316
score: 38.19
coord: 356..367
score: 37.38
IPR001128Cytochrome P450PFAMPF00067p450coord: 44..464
e-value: 2.5E-74
score: 250.7
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 308..334
score: 26.98
coord: 395..419
score: 24.28
coord: 288..305
score: 27.75
coord: 427..437
score: 53.27
coord: 355..373
score: 25.71
coord: 437..460
score: 30.54
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 38..488
e-value: 2.7E-106
score: 358.0
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 41..485
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 430..439

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy3G001750.2CsGy3G001750.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen