CsGy2G025700 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy2G025700
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionprotein NRT1/ PTR FAMILY 6.4
LocationGy14Chr2: 33163515 .. 33166623 (+)
RNA-Seq ExpressionCsGy2G025700
SyntenyCsGy2G025700
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCTAATCCCAATCATTCATAAACTTTTTGCTTTCCAATAAATCATGAGTCTAAGCATAACTATAGCACAAAGACAATCCCCTCAGCCTCTTGATCTCTTTCTTTCTCTCTAACAGAGGGAAATAAGCAAAGAAAAGGGAAAAAAAAATGGTAACTTGTTTATGGTCAAACTCCTTAGTTTCTATTGTCTATGAGATGGATTTTTTAACAGTTAAACTTTTCCTTTTTATCAACAGGTTCTGGTATCAAAACATGGAAGTGGAGGAGAAGAAGATGATACAGCAGTAGATTTTTGGGGAAACCCAGTTGACAAATCAAAAACAGGAGGATGGCTTGCAGCTGGGCTCATTTTAGGTGCTTGAAAATTTTGTTTTGTTTTGCACAGAATAAATAGCAATGCTTAAATGCCTAAAATCATGCAGGAACTGAACTCTCTGAAAGGATATGTGTGATGGGAATATCAATGAACTTAGTGACATATCTAGTTGGGGATTTGCATCTTACGTCAGCCAAATCTGCAACTATAGTCACTAATTTTCTTGGTGCTCTTAATCTTCTTGGGCTTCTTGGTGGCTTCTTAGCAGATGCCAAGCTTGGAAGGTACCTCACTGTTGCTATCTTTGCTTCCATTACTGCTGTGGTAAGTTCTAAGCCATTAAGAAATGACTTCAACAAAGAAAATTTCACTTCAGAATTTAGCTTGAGCCTTGCTGGTTTTTGTAGGGTGTTATTTTGTTAACATTGGCCACAACAATCCCTGGCATGAGACCTCCTCACTGTGATGACTCTACAAGGCAACTTCATCAATGCATTGAAGCTAATGGTGGGCAACTGGCCATGCTCTATGCTGCACTATATACTATAGCACTCGGTGGAGGGGGGATAAAATCAAATGTTTCAGGATTCGGGTCAGATCAATTTGACACTAATGATCCGAAGGAGGAGAAGGCTACGATATTCTTCTTCAATAGGTTCTACTTTGCGATAAGTATCGGGTCGTTGTTTGCTGTCATTGTGTTGGTTTATGTACAAGACAAAGTGGGGAGAGGTTGGGGATATGGAATCTCAGGAGGGACCATGGCGGTTGCTGTAATTGTACTGCTTTGTGGGACAACCTTTTACAGGTTTAAAAAGCCAAGAGGAAGCCCTCTCACAGTGATATGGAGAGTGGTTCTTTTGGCTTGGAAGAAGAGAAGTCTGCCTCACCCAGCTCATCCCAGCTTCTTAAATGACTACCAAAATGCCAAGGTTCCCTACACCTACAGGTTCAAGTAAGTGTTTTTCTGATCTATCATCAAATACTCTCTACTTTTTCGTTCCCTCTCTTTCACTCTCTGTATGTCCATGTAAAATTGATTATCTGTTTCTTGTTTTAGATTTGATGTATTATAAGACATTCTGCAAGATGCTATAGTTTATGAATAATCAACAAAAGAAAAAAAAAAGAATGTAGTGATGAAATCTAGAGTTTATATGCTAGAGTTGAAGAAACAATTGCTTAAAGTAGAGTTATAAGATAAAATTCTTCAATATTTATATAAGGCGAAGATAATGGAAAAGACGGAGTTGTTGAATAGGTACAAAAATCCAATAGTGATTACTATTGAAGTTGTGTTATTAAAATTGGTTCAATAAATGTACAAACATCAATAGTGTTTACAATTGAAGTTGTATTATTCATGAACAGGAAAAACATCCCATAATACATTACTAATCACACTTGTCTTTTACAACCAATCCTCGTATTGGTGCATTCACTTCACTTAATTTATTTGATTATATATTTATTTTGATTTGGGAACGTTGACTAATGAAAATATAAATATCAGATGTCTTGACAAGGCTGCAATTCTAGACGAGTATATATTTATTTTGATTTGGGAACGTTGACTAATGAAAATATAAATATCAGATGTCTTGACAAGGCTGCAATTCTAGACGAGTATGCTACCACTGGTCCAAACAATCCATGGATAGTCTCAACAGTGACTGAAGTTGAAGAAGTGAAAATGGTGTTCAAGCTCATACCCATTTGGTCCACTGGCATCCTCTTTTGGACGATCTACTCTCAAATGACTACATTCACAGTAGAGCAAGCATCATTTATGGAAAGAAAAGTTGGCAGTTTCGAGATTCCTTCTGGATCTATGTCAGCCTTTCTATTCATTGCCATCCTGCTCGTCACTTCCTTGAATGAGAAACTATTCATACCAATTGCTCGGAAGCTGACTCACAACGTACAAGGTCTCACAAGCCTTCAGAGAATTGGGATTGGACTTGTTTTCTCAATTTTTGGCATGGTGGCTGCTGGGGCTGTTGAGAAAGAGAGGAAGGCATCTGCTGTGGGACAAAGTACTCGTATAAGTGCTTTCTGGTTGATACCTCAGTTCTTCTTGGTCGGTGCTGGAGAAGCATTTACATATGTTGGACAACTCGAGTTTTTCATCAGAGAAGCACCGGAACAAATGAAGTCAATGAGCACGGGACTTTTTCTTAGCACTCTATCCATGGGATTTTTTGTTAGTAGTTTGTTGGTAACAATTGTAGATAAAGTAACCAACAAGCAATGGCTGACAAGTAATCTCAACAAGGGGCACTTGAGCTACTTCTATTGGCTACTCGCAGTGTTGGGCCTGCTAAACTTCTTCTTCTTTATTCTTCTTGCCCGAAAACATCAGTACAAAGACCAGCATTATGTCAGCTCCAAAGACAATAACGAGGAGCTCAAGATTTCAAATGACATGGTCGCACTTGAAATGGAAGGACCAATAAGCAAGAAAGCAGCAGAAACTTAAGAGTACGCTAAGGGTGCAAAATTTAGAACTCAAGGACACATTTGCACAGCATATAGAACAGATCCTATTCAAGTAAGGTCGCTGCTTTAAGGTGCCCCTTTTACAAGTTTAAAATCCTGCATGTACGTTTGAATATCTCAAAGCCTGTATCTACAAGACAACCATCTTGTGGTTTTAGCATTAATGAGCAGATGAGCCACTGCCATTTTGATTTCCCCTAGGCTTATATGATCTAAAGCATTAAGCATATTACAAATAATGTTCTTAAGTGCAAAAGGGCGTGCATAGCATTTATGCAAACAGCCGTGTTTCG

mRNA sequence

CTCCTAATCCCAATCATTCATAAACTTTTTGCTTTCCAATAAATCATGAGTCTAAGCATAACTATAGCACAAAGACAATCCCCTCAGCCTCTTGATCTCTTTCTTTCTCTCTAACAGAGGGAAATAAGCAAAGAAAAGGGAAAAAAAAATGGTTCTGGTATCAAAACATGGAAGTGGAGGAGAAGAAGATGATACAGCAGTAGATTTTTGGGGAAACCCAGTTGACAAATCAAAAACAGGAGGATGGCTTGCAGCTGGGCTCATTTTAGGAACTGAACTCTCTGAAAGGATATGTGTGATGGGAATATCAATGAACTTAGTGACATATCTAGTTGGGGATTTGCATCTTACGTCAGCCAAATCTGCAACTATAGTCACTAATTTTCTTGGTGCTCTTAATCTTCTTGGGCTTCTTGGTGGCTTCTTAGCAGATGCCAAGCTTGGAAGGTACCTCACTGTTGCTATCTTTGCTTCCATTACTGCTGTGGGTGTTATTTTGTTAACATTGGCCACAACAATCCCTGGCATGAGACCTCCTCACTGTGATGACTCTACAAGGCAACTTCATCAATGCATTGAAGCTAATGGTGGGCAACTGGCCATGCTCTATGCTGCACTATATACTATAGCACTCGGTGGAGGGGGGATAAAATCAAATGTTTCAGGATTCGGGTCAGATCAATTTGACACTAATGATCCGAAGGAGGAGAAGGCTACGATATTCTTCTTCAATAGGTTCTACTTTGCGATAAGTATCGGGTCGTTGTTTGCTGTCATTGTGTTGGTTTATGTACAAGACAAAGTGGGGAGAGGTTGGGGATATGGAATCTCAGGAGGGACCATGGCGGTTGCTGTAATTGTACTGCTTTGTGGGACAACCTTTTACAGGTTTAAAAAGCCAAGAGGAAGCCCTCTCACAGTGATATGGAGAGTGGTTCTTTTGGCTTGGAAGAAGAGAAGTCTGCCTCACCCAGCTCATCCCAGCTTCTTAAATGACTACCAAAATGCCAAGGTTCCCTACACCTACAGGTTCAAATGTCTTGACAAGGCTGCAATTCTAGACGAGTATGCTACCACTGGTCCAAACAATCCATGGATAGTCTCAACAGTGACTGAAGTTGAAGAAGTGAAAATGGTGTTCAAGCTCATACCCATTTGGTCCACTGGCATCCTCTTTTGGACGATCTACTCTCAAATGACTACATTCACAGTAGAGCAAGCATCATTTATGGAAAGAAAAGTTGGCAGTTTCGAGATTCCTTCTGGATCTATGTCAGCCTTTCTATTCATTGCCATCCTGCTCGTCACTTCCTTGAATGAGAAACTATTCATACCAATTGCTCGGAAGCTGACTCACAACGTACAAGGTCTCACAAGCCTTCAGAGAATTGGGATTGGACTTGTTTTCTCAATTTTTGGCATGGTGGCTGCTGGGGCTGTTGAGAAAGAGAGGAAGGCATCTGCTGTGGGACAAAGTACTCGTATAAGTGCTTTCTGGTTGATACCTCAGTTCTTCTTGGTCGGTGCTGGAGAAGCATTTACATATGTTGGACAACTCGAGTTTTTCATCAGAGAAGCACCGGAACAAATGAAGTCAATGAGCACGGGACTTTTTCTTAGCACTCTATCCATGGGATTTTTTGTTAGTAGTTTGTTGGTAACAATTGTAGATAAAGTAACCAACAAGCAATGGCTGACAAGTAATCTCAACAAGGGGCACTTGAGCTACTTCTATTGGCTACTCGCAGTGTTGGGCCTGCTAAACTTCTTCTTCTTTATTCTTCTTGCCCGAAAACATCAGTACAAAGACCAGCATTATGTCAGCTCCAAAGACAATAACGAGGAGCTCAAGATTTCAAATGACATGGTCGCACTTGAAATGGAAGGACCAATAAGCAAGAAAGCAGCAGAAACTTAAGAGTACGCTAAGGGTGCAAAATTTAGAACTCAAGGACACATTTGCACAGCATATAGAACAGATCCTATTCAAGTAAGGTCGCTGCTTTAAGGTGCCCCTTTTACAAGTTTAAAATCCTGCATGTACGTTTGAATATCTCAAAGCCTGTATCTACAAGACAACCATCTTGTGGTTTTAGCATTAATGAGCAGATGAGCCACTGCCATTTTGATTTCCCCTAGGCTTATATGATCTAAAGCATTAAGCATATTACAAATAATGTTCTTAAGTGCAAAAGGGCGTGCATAGCATTTATGCAAACAGCCGTGTTTCG

Coding sequence (CDS)

ATGGTTCTGGTATCAAAACATGGAAGTGGAGGAGAAGAAGATGATACAGCAGTAGATTTTTGGGGAAACCCAGTTGACAAATCAAAAACAGGAGGATGGCTTGCAGCTGGGCTCATTTTAGGAACTGAACTCTCTGAAAGGATATGTGTGATGGGAATATCAATGAACTTAGTGACATATCTAGTTGGGGATTTGCATCTTACGTCAGCCAAATCTGCAACTATAGTCACTAATTTTCTTGGTGCTCTTAATCTTCTTGGGCTTCTTGGTGGCTTCTTAGCAGATGCCAAGCTTGGAAGGTACCTCACTGTTGCTATCTTTGCTTCCATTACTGCTGTGGGTGTTATTTTGTTAACATTGGCCACAACAATCCCTGGCATGAGACCTCCTCACTGTGATGACTCTACAAGGCAACTTCATCAATGCATTGAAGCTAATGGTGGGCAACTGGCCATGCTCTATGCTGCACTATATACTATAGCACTCGGTGGAGGGGGGATAAAATCAAATGTTTCAGGATTCGGGTCAGATCAATTTGACACTAATGATCCGAAGGAGGAGAAGGCTACGATATTCTTCTTCAATAGGTTCTACTTTGCGATAAGTATCGGGTCGTTGTTTGCTGTCATTGTGTTGGTTTATGTACAAGACAAAGTGGGGAGAGGTTGGGGATATGGAATCTCAGGAGGGACCATGGCGGTTGCTGTAATTGTACTGCTTTGTGGGACAACCTTTTACAGGTTTAAAAAGCCAAGAGGAAGCCCTCTCACAGTGATATGGAGAGTGGTTCTTTTGGCTTGGAAGAAGAGAAGTCTGCCTCACCCAGCTCATCCCAGCTTCTTAAATGACTACCAAAATGCCAAGGTTCCCTACACCTACAGGTTCAAATGTCTTGACAAGGCTGCAATTCTAGACGAGTATGCTACCACTGGTCCAAACAATCCATGGATAGTCTCAACAGTGACTGAAGTTGAAGAAGTGAAAATGGTGTTCAAGCTCATACCCATTTGGTCCACTGGCATCCTCTTTTGGACGATCTACTCTCAAATGACTACATTCACAGTAGAGCAAGCATCATTTATGGAAAGAAAAGTTGGCAGTTTCGAGATTCCTTCTGGATCTATGTCAGCCTTTCTATTCATTGCCATCCTGCTCGTCACTTCCTTGAATGAGAAACTATTCATACCAATTGCTCGGAAGCTGACTCACAACGTACAAGGTCTCACAAGCCTTCAGAGAATTGGGATTGGACTTGTTTTCTCAATTTTTGGCATGGTGGCTGCTGGGGCTGTTGAGAAAGAGAGGAAGGCATCTGCTGTGGGACAAAGTACTCGTATAAGTGCTTTCTGGTTGATACCTCAGTTCTTCTTGGTCGGTGCTGGAGAAGCATTTACATATGTTGGACAACTCGAGTTTTTCATCAGAGAAGCACCGGAACAAATGAAGTCAATGAGCACGGGACTTTTTCTTAGCACTCTATCCATGGGATTTTTTGTTAGTAGTTTGTTGGTAACAATTGTAGATAAAGTAACCAACAAGCAATGGCTGACAAGTAATCTCAACAAGGGGCACTTGAGCTACTTCTATTGGCTACTCGCAGTGTTGGGCCTGCTAAACTTCTTCTTCTTTATTCTTCTTGCCCGAAAACATCAGTACAAAGACCAGCATTATGTCAGCTCCAAAGACAATAACGAGGAGCTCAAGATTTCAAATGACATGGTCGCACTTGAAATGGAAGGACCAATAAGCAAGAAAGCAGCAGAAACTTAA

Protein sequence

MVLVSKHGSGGEEDDTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTLATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKEEKATIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLLCGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDKAAILDEYATTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASFMERKVGSFEIPSGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVFSIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLTSNLNKGHLSYFYWLLAVLGLLNFFFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET*
Homology
BLAST of CsGy2G025700 vs. ExPASy Swiss-Prot
Match: Q9LVE0 (Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1)

HSP 1 Score: 802.0 bits (2070), Expect = 4.6e-231
Identity = 405/558 (72.58%), Postives = 458/558 (82.08%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MV VS      +  + A D+ GNP DKSKTGGWL AGLILG+ELSERICVMGISMNLVTY
Sbjct: 1   MVHVSSSHGAKDGSEEAYDYRGNPPDKSKTGGWLGAGLILGSELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL 120
           LVGDLH++SAKSATIVTNF+G LNLLGLLGGFLADAKLGRY  VAI AS+TA+GV+LLT+
Sbjct: 61  LVGDLHISSAKSATIVTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASVTALGVLLLTV 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTI  MRPP CDD  R  HQCIEANG QLA+LY ALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTISSMRPPICDDFRRLHHQCIEANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKATIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL 240
           T+DPKEEK  IFFFNRFYF+IS+GSLFAVI LVYVQD VGRGWGYGIS  TM VA IVLL
Sbjct: 181 TSDPKEEKQMIFFFNRFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300
           CGT  YRFKKP+GSP T IWRV  LAWKKR   +PAHPS LN Y N  VP+T   KCLDK
Sbjct: 241 CGTKRYRFKKPKGSPFTTIWRVGFLAWKKRKESYPAHPSLLNGYDNTTVPHTEMLKCLDK 300

Query: 301 AAILDEYATTG-----PNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTV 360
           AAI    ++         +PWIVSTVT+VEEVK+V KL+PIW+T ILFWTIYSQMTTFTV
Sbjct: 301 AAISKNESSPSSKDFEEKDPWIVSTVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTV 360

Query: 361 EQASFMERKVGSFEIPSGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIG 420
           EQA+FM+RK+GSF +P+GS SAFL + ILL TSLNE++F+P+ R+LT   QG+TSLQRIG
Sbjct: 361 EQATFMDRKLGSFTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIG 420

Query: 421 IGLVFSIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIR 480
           +GLVFS+  M  A  +E  R+ +AV    +ISAFWL+PQ+FLVGAGEAF YVGQLEFFIR
Sbjct: 421 VGLVFSMAAMAVAAVIENARREAAVNNDKKISAFWLVPQYFLVGAGEAFAYVGQLEFFIR 480

Query: 481 EAPEQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLTSNLNKGHLSYFYWLLAVL 540
           EAPE+MKSMSTGLFLST+SMGFFVSSLLV++VD+VT+K WL SNLNK  L+YFYWLL VL
Sbjct: 481 EAPERMKSMSTGLFLSTISMGFFVSSLLVSLVDRVTDKSWLRSNLNKARLNYFYWLLVVL 540

Query: 541 GLLNFFFFILLARKHQYK 554
           G LNF  FI+ A KHQYK
Sbjct: 541 GALNFLIFIVFAMKHQYK 558

BLAST of CsGy2G025700 vs. ExPASy Swiss-Prot
Match: Q05085 (Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 2.6e-149
Identity = 285/567 (50.26%), Postives = 384/567 (67.72%), Query Frame = 0

Query: 12  EEDDTAVDFW---GNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLT 71
           + DD  +D W   G P D+SKTGGW +A +IL  E  ER+  +GI +NLVTYL G +HL 
Sbjct: 7   KSDDILLDAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMHLG 66

Query: 72  SAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTLATTIPGMR 131
           +A +A  VTNFLG   +L LLGGF+AD  LGRYLT+AIFA+I A GV +LTL+T IPG+R
Sbjct: 67  NATAANTVTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPGLR 126

Query: 132 PPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKEEK 191
           PP C+ +T     C +A+G QL +LY ALY  ALG GG+K++VSGFGSDQFD  +PKE  
Sbjct: 127 PPRCNPTTSS--HCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERS 186

Query: 192 ATIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLLCGTTFYRF 251
              +FFNRF+F I++GSL AV VLVYVQD VGR WGYGI    + +A+ V L GT  YRF
Sbjct: 187 KMTYFFNRFFFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRF 246

Query: 252 KKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQN-----------AKVPYTYRFKC 311
           KK  GSP+T +  V++ AW+ R L  PA PS+L D  +            K+P+T +F+ 
Sbjct: 247 KKLIGSPMTQVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRS 306

Query: 312 LDKAAILDEYA--TTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTV 371
           LDKAAI D+ A  T+   N W +ST+T+VEEVK + +++PIW+T ILFWT+++Q+TT +V
Sbjct: 307 LDKAAIRDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSV 366

Query: 372 EQASFMERKVGSFEIPSGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIG 431
            Q+  ++R +GSFEIP  SM+ F    +LL T++ +++ I + +KL +   GL  LQRIG
Sbjct: 367 AQSETLDRSIGSFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIG 426

Query: 432 IGLVFSIFGMVAAGAVE--KERKASAVGQSTR---ISAFWLIPQFFLVGAGEAFTYVGQL 491
           +GL F    M  A  VE  + R A A G + +   +  + LIPQ+ +VG GEA  Y GQL
Sbjct: 427 LGLFFGSMAMAVAALVELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQL 486

Query: 492 EFFIREAPEQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK--QWLTSNLNKGHLSYF 551
           +FF+RE P+ MK MSTGL LSTL++GFF SS+LVTIV+K T K   W+  +LNKG L  F
Sbjct: 487 DFFLRECPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGKAHPWIADDLNKGRLYNF 546

Query: 552 YWLLAVLGLLNFFFFILLARKHQYKDQ 556
           YWL+AVL  LNF  F++ ++ + YK++
Sbjct: 547 YWLVAVLVALNFLIFLVFSKWYVYKEK 571

BLAST of CsGy2G025700 vs. ExPASy Swiss-Prot
Match: Q9SZY4 (Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 2.9e-148
Identity = 283/546 (51.83%), Postives = 381/546 (69.78%), Query Frame = 0

Query: 17  AVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLTSAKSATIV 76
           AVD+ G P DKSKTGGW+ A LILG E+ ER+  MGI++NLVTYL+  +HL S+ SA IV
Sbjct: 12  AVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETMHLPSSTSANIV 71

Query: 77  TNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTLATTIPGMRPPHCDDST 136
           T+F+G   LL LLGGFLAD+ LGR+ T+ IF++I A+G   L +AT +P +RPP C    
Sbjct: 72  TDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLPELRPPTCHHG- 131

Query: 137 RQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKEEKATIFFFNR 196
                CI A   Q+ +LY +LY IALG GG+KS++SGFGSDQFD  DPKE+    FFFNR
Sbjct: 132 ---EACIPATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAHMAFFFNR 191

Query: 197 FYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLLCGTTFYRFKKPRGSPL 256
           F+F IS+G+L AV VLVY+QD+VGR W YGI   +MA+A+++ LCGT  YR+KK +GSP+
Sbjct: 192 FFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYKKSQGSPV 251

Query: 257 TVIWRVVLLAWKKRSLPHPAHPSFL--NDYQNAKVPYTYRFKCLDKAAILDE------YA 316
             I++V+  A++KR +  P    +L  ++ +  ++ +T +F  LDKAAI+ E        
Sbjct: 252 VQIFQVIAAAFRKRKMELPQSIVYLYEDNPEGIRIEHTDQFHLLDKAAIVAEGDFEQTLD 311

Query: 317 TTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASFMERKVGSF 376
                NPW +S+VT+VEEVKM+ +L+PIW+T I+FWT Y+QM TF+VEQAS M R +GSF
Sbjct: 312 GVAIPNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQASTMRRNIGSF 371

Query: 377 EIPSGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVFSIFGMVAA 436
           +IP+GS++ F   AIL+  ++ ++  +P  +K      G +SLQRI IGLV S  GM AA
Sbjct: 372 KIPAGSLTVFFVAAILITLAVYDRAIMPFWKK-WKGKPGFSSLQRIAIGLVLSTAGMAAA 431

Query: 437 GAVEKERKASAVGQSTR---ISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQMKSMS 496
             VE++R + A   S +   IS F L+PQFFLVGAGEAF Y GQL+FFI ++P+ MK+MS
Sbjct: 432 ALVEQKRLSVAKSSSQKTLPISVFLLVPQFFLVGAGEAFIYTGQLDFFITQSPKGMKTMS 491

Query: 497 TGLFLSTLSMGFFVSSLLVTIVDKVTNKQ----WLTSNLNKGHLSYFYWLLAVLGLLNFF 548
           TGLFL+TLS+GFFVSS LV+IV +VT+      WL  N+N G L YFYWLL +L  +NF 
Sbjct: 492 TGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRLDYFYWLLVILSGINFV 551

BLAST of CsGy2G025700 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 7.8e-122
Identity = 243/560 (43.39%), Postives = 351/560 (62.68%), Query Frame = 0

Query: 15  DTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLTSAKSAT 74
           D  VD   NP +K KTG W A   ILG E  ER+   G+  NLV YL   L+  +A +A 
Sbjct: 10  DGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAAN 69

Query: 75  IVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTLATTIPGMRPPHCDD 134
            VTN+ G   +  L+G F+ADA LGRY T+A F  I   G+ LLTL+ ++PG++P +C+ 
Sbjct: 70  NVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCNA 129

Query: 135 STRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKEEKATIFFF 194
            T         N  Q A+ + ALY IALG GGIK  VS FG+DQFD ND  E+     FF
Sbjct: 130 DT------CHPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFF 189

Query: 195 NRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLLCGTTFYRFKKPRGS 254
           N FYF+I++G+L A  VLV++Q  VG GWG+G+    M +AV     G+ FYR ++P GS
Sbjct: 190 NWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGS 249

Query: 255 PLTVIWRVVLLAWKKRSLPHPAHPSFL--------NDYQNAKVPYTYRFKCLDKAAI--L 314
           PLT I++V++ A++K S+  P   S L        N   + K+ +T   K  DKAA+   
Sbjct: 250 PLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQ 309

Query: 315 DEYATTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASFMERK 374
            +    G  NPW + +VT+VEE+K +  L+P+W+TGI+F T+YSQM+T  V Q + M++ 
Sbjct: 310 SDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQH 369

Query: 375 VG-SFEIPSGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVFSIF 434
           +G +FEIPS S+S F  +++L  T + ++  IP+ARK T N +G T LQR+GIGLV SIF
Sbjct: 370 MGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIF 429

Query: 435 GMVAAGAVEKER-----KASAVGQ-STRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREA 494
            M+ AG +E  R       +A  Q    +S FW IPQ+ L+G  E FT++GQLEFF  +A
Sbjct: 430 AMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQA 489

Query: 495 PEQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQ----WLTSNLNKGHLSYFYWLLA 554
           P+ M+S+ + L L+T+++G ++S++LVT+V K+T K     W+  NLN+GHL YF++LLA
Sbjct: 490 PDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYLLA 549

BLAST of CsGy2G025700 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 7.1e-115
Identity = 234/560 (41.79%), Postives = 343/560 (61.25%), Query Frame = 0

Query: 15  DTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLTSAKSAT 74
           D  +D    P +K+KTG W A   ILGTE  ER+   G+S NL+ YL   +++ +  ++ 
Sbjct: 11  DGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSASK 70

Query: 75  IVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTLATTIPGMRPPHCDD 134
            V+N+ G      L+G F+ADA LGRY T+A F  I   G+ LLT++ ++PG+ P  C  
Sbjct: 71  SVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGLTPT-CSG 130

Query: 135 STRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKEEKATIFFF 194
            T        A  GQ A+ + ALY IALG GGIK  VS FG+DQFD  D KE+++   FF
Sbjct: 131 ET------CHATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFF 190

Query: 195 NRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLLCGTTFYRFKKPRGS 254
           N FYF I++G++ A  VLV++Q  VG GWG G+    MA+AV+    G+ FYR +KP GS
Sbjct: 191 NWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGS 250

Query: 255 PLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNA--------KVPYTYRFKCLDKAAILDE 314
           PLT + +V++ + +K  +  P   S L + Q+A        K+ +T      DKAA+  E
Sbjct: 251 PLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAVETE 310

Query: 315 YATTG--PNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASFMERK 374
               G   ++ W + TVT+VEE+K + +L+PIW+TGI+F ++YSQM T  V Q + +++ 
Sbjct: 311 SDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQH 370

Query: 375 VG-SFEIPSGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVFSIF 434
           +G +F+IPS S+S F  +++L    + +KL +P ARK T + +G T LQRIGIGLV SIF
Sbjct: 371 MGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIF 430

Query: 435 GMVAAGAVEKERKASAV------GQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREA 494
            MV+AG +E  R            ++  ++ FW +PQ+FLVG  E FT++GQLEFF  +A
Sbjct: 431 SMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQA 490

Query: 495 PEQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK----QWLTSNLNKGHLSYFYWLLA 554
           P+ M+S+ + L L+ ++ G ++S+ LVT+V KVT       W+  NLN GHL YF+WLLA
Sbjct: 491 PDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFFWLLA 550

BLAST of CsGy2G025700 vs. NCBI nr
Match: AFR11353.1 (nitrate transporter [Cucumis sativus])

HSP 1 Score: 1141 bits (2951), Expect = 0.0
Identity = 585/589 (99.32%), Postives = 585/589 (99.32%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGGEEDDTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGEEDDTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL 120
           LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL
Sbjct: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKATIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL 240
           TNDPK EKA IFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL
Sbjct: 181 TNDPKGEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYATTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF 360
           AAILDEYATTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF
Sbjct: 301 AAILDEYATTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF 360

Query: 361 MERKVGSFEIPSGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF 420
           MERKVGSFEIP GSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF
Sbjct: 361 MERKVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF 420

Query: 421 SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQ 480
           SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQ
Sbjct: 421 SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQ 480

Query: 481 MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLTSNLNKGHLSYFYWLLAVLGLLNF 540
           MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWL SNLNKGHLSYFYWLLAVLGLLNF
Sbjct: 481 MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNKGHLSYFYWLLAVLGLLNF 540

Query: 541 FFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET 589
           FFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET
Sbjct: 541 FFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET 589

BLAST of CsGy2G025700 vs. NCBI nr
Match: XP_011649985.1 (protein NRT1/ PTR FAMILY 6.4 [Cucumis sativus] >KGN63217.1 hypothetical protein Csa_022407 [Cucumis sativus])

HSP 1 Score: 1139 bits (2946), Expect = 0.0
Identity = 584/589 (99.15%), Postives = 584/589 (99.15%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGGEEDD AVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGEEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL 120
           LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL
Sbjct: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKATIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL 240
           TNDPK EKA IFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL
Sbjct: 181 TNDPKGEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYATTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF 360
           AAILDEYATTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF
Sbjct: 301 AAILDEYATTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF 360

Query: 361 MERKVGSFEIPSGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF 420
           MERKVGSFEIP GSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF
Sbjct: 361 MERKVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF 420

Query: 421 SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQ 480
           SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQ
Sbjct: 421 SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQ 480

Query: 481 MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLTSNLNKGHLSYFYWLLAVLGLLNF 540
           MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWL SNLNKGHLSYFYWLLAVLGLLNF
Sbjct: 481 MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNKGHLSYFYWLLAVLGLLNF 540

Query: 541 FFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET 589
           FFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET
Sbjct: 541 FFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET 589

BLAST of CsGy2G025700 vs. NCBI nr
Match: KAA0062862.1 (protein NRT1/ PTR FAMILY 6.4 [Cucumis melo var. makuwa])

HSP 1 Score: 1121 bits (2899), Expect = 0.0
Identity = 571/589 (96.94%), Postives = 579/589 (98.30%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGG EDD AVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGGEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL 120
           LVG+LHLTSAKSATIVTNFLGALNLLGLLGGFLADA+LGRYLTVA FASITAVGVILLTL
Sbjct: 61  LVGELHLTSAKSATIVTNFLGALNLLGLLGGFLADARLGRYLTVATFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKATIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL 240
           TNDPKEEKA IFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTM +AVIVLL
Sbjct: 181 TNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMVIAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYATTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF 360
           AAI+DEYATT PNNPWIVSTVTE+EEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF
Sbjct: 301 AAIIDEYATTDPNNPWIVSTVTEIEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF 360

Query: 361 MERKVGSFEIPSGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF 420
           MER+VGSFEIP GSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF
Sbjct: 361 MERRVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF 420

Query: 421 SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQ 480
           SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAF YVGQLEFFIREAPEQ
Sbjct: 421 SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAPEQ 480

Query: 481 MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLTSNLNKGHLSYFYWLLAVLGLLNF 540
           MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK+WL SNLNKGHLSYFYWLLAVLGLLNF
Sbjct: 481 MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKRWLRSNLNKGHLSYFYWLLAVLGLLNF 540

Query: 541 FFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET 589
           FFF+LLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPIS KAAET
Sbjct: 541 FFFLLLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISMKAAET 589

BLAST of CsGy2G025700 vs. NCBI nr
Match: XP_008455932.1 (PREDICTED: protein NRT1/ PTR FAMILY 6.4 [Cucumis melo] >TYK09533.1 protein NRT1/ PTR FAMILY 6.4 [Cucumis melo var. makuwa])

HSP 1 Score: 1121 bits (2899), Expect = 0.0
Identity = 571/589 (96.94%), Postives = 579/589 (98.30%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGG EDD AVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGGEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL 120
           LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADA+LGRYLTVA FASITAVGVILLTL
Sbjct: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADARLGRYLTVATFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKATIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL 240
           TNDPKEEKA IFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTM +AVIVLL
Sbjct: 181 TNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMVIAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYATTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF 360
           AAI+DEYATT PNNPWIVSTVTE+EEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF
Sbjct: 301 AAIIDEYATTDPNNPWIVSTVTEIEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF 360

Query: 361 MERKVGSFEIPSGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF 420
           MER+VGSFEIP GSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF
Sbjct: 361 MERRVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF 420

Query: 421 SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQ 480
           SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAF YVGQLEFFIREAPEQ
Sbjct: 421 SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAPEQ 480

Query: 481 MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLTSNLNKGHLSYFYWLLAVLGLLNF 540
           MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK+WL SNLNKGHLSYFYWLLAVLGLLNF
Sbjct: 481 MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKRWLRSNLNKGHLSYFYWLLAVLGLLNF 540

Query: 541 FFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET 589
           FFF+LLARKHQYKDQHYVSSKDNNEELKISNDMVALE+EGPIS KAAET
Sbjct: 541 FFFLLLARKHQYKDQHYVSSKDNNEELKISNDMVALEIEGPISMKAAET 589

BLAST of CsGy2G025700 vs. NCBI nr
Match: XP_038901295.1 (protein NRT1/ PTR FAMILY 6.4 [Benincasa hispida])

HSP 1 Score: 1069 bits (2765), Expect = 0.0
Identity = 548/589 (93.04%), Postives = 559/589 (94.91%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGG EDD AVDFWGNPVDKSK GGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGGEDDAAVDFWGNPVDKSKRGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL 120
           LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVA FASITAVGVILLTL
Sbjct: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVASFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPP CD STRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPQCDYSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKATIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL 240
           TNDPKEEKA IFFFNRFYFAISIGSLF+V VLVYVQD VGRGWGYGISGGTM +AVIVLL
Sbjct: 181 TNDPKEEKAMIFFFNRFYFAISIGSLFSVTVLVYVQDHVGRGWGYGISGGTMVIAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300
           CGT FYRFKKPRGSPLTVIWRVVLLAWKKRSLPH AHPSFLNDYQNAKVPYTY+FKCLDK
Sbjct: 241 CGTYFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHAAHPSFLNDYQNAKVPYTYKFKCLDK 300

Query: 301 AAILDEYATTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF 360
           AAILDEYA T PNNPWIVSTVTE+EEVKMV KLIPIWSTGILFWTIYSQMTTFTV QASF
Sbjct: 301 AAILDEYAVTDPNNPWIVSTVTEIEEVKMVLKLIPIWSTGILFWTIYSQMTTFTVVQASF 360

Query: 361 MERKVGSFEIPSGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF 420
           MER VGSFEIP+GSMSAFLFI ILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGL F
Sbjct: 361 MERNVGSFEIPAGSMSAFLFITILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLFF 420

Query: 421 SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQ 480
           SIFGMVAAG VEKERK  AV Q+TRISAFWLIPQFFLVGAGEAF YVGQLEFFIREAPE+
Sbjct: 421 SIFGMVAAGVVEKERKTFAVEQNTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAPER 480

Query: 481 MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLTSNLNKGHLSYFYWLLAVLGLLNF 540
           MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWL +NLNKG L+YFYWLLAVLGLLNF
Sbjct: 481 MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRNNLNKGDLNYFYWLLAVLGLLNF 540

Query: 541 FFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET 589
           FFF+LLARKHQYKDQHYVSSKDNNEELK SNDM+ALEMEG IS KA ET
Sbjct: 541 FFFLLLARKHQYKDQHYVSSKDNNEELKTSNDMIALEMEGSISIKATET 589

BLAST of CsGy2G025700 vs. ExPASy TrEMBL
Match: J7LEY9 (Nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT1.3 PE=2 SV=1)

HSP 1 Score: 1141 bits (2951), Expect = 0.0
Identity = 585/589 (99.32%), Postives = 585/589 (99.32%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGGEEDDTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGEEDDTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL 120
           LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL
Sbjct: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKATIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL 240
           TNDPK EKA IFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL
Sbjct: 181 TNDPKGEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYATTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF 360
           AAILDEYATTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF
Sbjct: 301 AAILDEYATTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF 360

Query: 361 MERKVGSFEIPSGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF 420
           MERKVGSFEIP GSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF
Sbjct: 361 MERKVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF 420

Query: 421 SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQ 480
           SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQ
Sbjct: 421 SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQ 480

Query: 481 MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLTSNLNKGHLSYFYWLLAVLGLLNF 540
           MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWL SNLNKGHLSYFYWLLAVLGLLNF
Sbjct: 481 MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNKGHLSYFYWLLAVLGLLNF 540

Query: 541 FFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET 589
           FFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET
Sbjct: 541 FFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET 589

BLAST of CsGy2G025700 vs. ExPASy TrEMBL
Match: A0A0A0LQY5 (Oligopeptide transporter OS=Cucumis sativus OX=3659 GN=Csa_2G416080 PE=3 SV=1)

HSP 1 Score: 1139 bits (2946), Expect = 0.0
Identity = 584/589 (99.15%), Postives = 584/589 (99.15%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGGEEDD AVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGEEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL 120
           LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL
Sbjct: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKATIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL 240
           TNDPK EKA IFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL
Sbjct: 181 TNDPKGEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYATTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF 360
           AAILDEYATTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF
Sbjct: 301 AAILDEYATTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF 360

Query: 361 MERKVGSFEIPSGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF 420
           MERKVGSFEIP GSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF
Sbjct: 361 MERKVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF 420

Query: 421 SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQ 480
           SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQ
Sbjct: 421 SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQ 480

Query: 481 MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLTSNLNKGHLSYFYWLLAVLGLLNF 540
           MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWL SNLNKGHLSYFYWLLAVLGLLNF
Sbjct: 481 MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNKGHLSYFYWLLAVLGLLNF 540

Query: 541 FFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET 589
           FFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET
Sbjct: 541 FFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET 589

BLAST of CsGy2G025700 vs. ExPASy TrEMBL
Match: A0A5A7V3P8 (Protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold2406G00170 PE=3 SV=1)

HSP 1 Score: 1121 bits (2899), Expect = 0.0
Identity = 571/589 (96.94%), Postives = 579/589 (98.30%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGG EDD AVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGGEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL 120
           LVG+LHLTSAKSATIVTNFLGALNLLGLLGGFLADA+LGRYLTVA FASITAVGVILLTL
Sbjct: 61  LVGELHLTSAKSATIVTNFLGALNLLGLLGGFLADARLGRYLTVATFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKATIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL 240
           TNDPKEEKA IFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTM +AVIVLL
Sbjct: 181 TNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMVIAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYATTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF 360
           AAI+DEYATT PNNPWIVSTVTE+EEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF
Sbjct: 301 AAIIDEYATTDPNNPWIVSTVTEIEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF 360

Query: 361 MERKVGSFEIPSGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF 420
           MER+VGSFEIP GSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF
Sbjct: 361 MERRVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF 420

Query: 421 SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQ 480
           SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAF YVGQLEFFIREAPEQ
Sbjct: 421 SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAPEQ 480

Query: 481 MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLTSNLNKGHLSYFYWLLAVLGLLNF 540
           MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK+WL SNLNKGHLSYFYWLLAVLGLLNF
Sbjct: 481 MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKRWLRSNLNKGHLSYFYWLLAVLGLLNF 540

Query: 541 FFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET 589
           FFF+LLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPIS KAAET
Sbjct: 541 FFFLLLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISMKAAET 589

BLAST of CsGy2G025700 vs. ExPASy TrEMBL
Match: A0A5D3CCF7 (Protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold757G00100 PE=3 SV=1)

HSP 1 Score: 1121 bits (2899), Expect = 0.0
Identity = 571/589 (96.94%), Postives = 579/589 (98.30%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGG EDD AVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGGEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL 120
           LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADA+LGRYLTVA FASITAVGVILLTL
Sbjct: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADARLGRYLTVATFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKATIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL 240
           TNDPKEEKA IFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTM +AVIVLL
Sbjct: 181 TNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMVIAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYATTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF 360
           AAI+DEYATT PNNPWIVSTVTE+EEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF
Sbjct: 301 AAIIDEYATTDPNNPWIVSTVTEIEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF 360

Query: 361 MERKVGSFEIPSGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF 420
           MER+VGSFEIP GSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF
Sbjct: 361 MERRVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF 420

Query: 421 SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQ 480
           SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAF YVGQLEFFIREAPEQ
Sbjct: 421 SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAPEQ 480

Query: 481 MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLTSNLNKGHLSYFYWLLAVLGLLNF 540
           MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK+WL SNLNKGHLSYFYWLLAVLGLLNF
Sbjct: 481 MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKRWLRSNLNKGHLSYFYWLLAVLGLLNF 540

Query: 541 FFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET 589
           FFF+LLARKHQYKDQHYVSSKDNNEELKISNDMVALE+EGPIS KAAET
Sbjct: 541 FFFLLLARKHQYKDQHYVSSKDNNEELKISNDMVALEIEGPISMKAAET 589

BLAST of CsGy2G025700 vs. ExPASy TrEMBL
Match: A0A1S3C263 (protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo OX=3656 GN=LOC103495995 PE=3 SV=1)

HSP 1 Score: 1121 bits (2899), Expect = 0.0
Identity = 571/589 (96.94%), Postives = 579/589 (98.30%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGG EDD AVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGGEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL 120
           LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADA+LGRYLTVA FASITAVGVILLTL
Sbjct: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADARLGRYLTVATFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKATIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL 240
           TNDPKEEKA IFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTM +AVIVLL
Sbjct: 181 TNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMVIAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYATTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF 360
           AAI+DEYATT PNNPWIVSTVTE+EEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF
Sbjct: 301 AAIIDEYATTDPNNPWIVSTVTEIEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASF 360

Query: 361 MERKVGSFEIPSGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF 420
           MER+VGSFEIP GSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF
Sbjct: 361 MERRVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVF 420

Query: 421 SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQ 480
           SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAF YVGQLEFFIREAPEQ
Sbjct: 421 SIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAPEQ 480

Query: 481 MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLTSNLNKGHLSYFYWLLAVLGLLNF 540
           MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK+WL SNLNKGHLSYFYWLLAVLGLLNF
Sbjct: 481 MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKRWLRSNLNKGHLSYFYWLLAVLGLLNF 540

Query: 541 FFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET 589
           FFF+LLARKHQYKDQHYVSSKDNNEELKISNDMVALE+EGPIS KAAET
Sbjct: 541 FFFLLLARKHQYKDQHYVSSKDNNEELKISNDMVALEIEGPISMKAAET 589

BLAST of CsGy2G025700 vs. TAIR 10
Match: AT3G21670.1 (Major facilitator superfamily protein )

HSP 1 Score: 802.0 bits (2070), Expect = 3.3e-232
Identity = 405/558 (72.58%), Postives = 458/558 (82.08%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MV VS      +  + A D+ GNP DKSKTGGWL AGLILG+ELSERICVMGISMNLVTY
Sbjct: 1   MVHVSSSHGAKDGSEEAYDYRGNPPDKSKTGGWLGAGLILGSELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL 120
           LVGDLH++SAKSATIVTNF+G LNLLGLLGGFLADAKLGRY  VAI AS+TA+GV+LLT+
Sbjct: 61  LVGDLHISSAKSATIVTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASVTALGVLLLTV 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTI  MRPP CDD  R  HQCIEANG QLA+LY ALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTISSMRPPICDDFRRLHHQCIEANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKATIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL 240
           T+DPKEEK  IFFFNRFYF+IS+GSLFAVI LVYVQD VGRGWGYGIS  TM VA IVLL
Sbjct: 181 TSDPKEEKQMIFFFNRFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300
           CGT  YRFKKP+GSP T IWRV  LAWKKR   +PAHPS LN Y N  VP+T   KCLDK
Sbjct: 241 CGTKRYRFKKPKGSPFTTIWRVGFLAWKKRKESYPAHPSLLNGYDNTTVPHTEMLKCLDK 300

Query: 301 AAILDEYATTG-----PNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTV 360
           AAI    ++         +PWIVSTVT+VEEVK+V KL+PIW+T ILFWTIYSQMTTFTV
Sbjct: 301 AAISKNESSPSSKDFEEKDPWIVSTVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTV 360

Query: 361 EQASFMERKVGSFEIPSGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIG 420
           EQA+FM+RK+GSF +P+GS SAFL + ILL TSLNE++F+P+ R+LT   QG+TSLQRIG
Sbjct: 361 EQATFMDRKLGSFTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIG 420

Query: 421 IGLVFSIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIR 480
           +GLVFS+  M  A  +E  R+ +AV    +ISAFWL+PQ+FLVGAGEAF YVGQLEFFIR
Sbjct: 421 VGLVFSMAAMAVAAVIENARREAAVNNDKKISAFWLVPQYFLVGAGEAFAYVGQLEFFIR 480

Query: 481 EAPEQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLTSNLNKGHLSYFYWLLAVL 540
           EAPE+MKSMSTGLFLST+SMGFFVSSLLV++VD+VT+K WL SNLNK  L+YFYWLL VL
Sbjct: 481 EAPERMKSMSTGLFLSTISMGFFVSSLLVSLVDRVTDKSWLRSNLNKARLNYFYWLLVVL 540

Query: 541 GLLNFFFFILLARKHQYK 554
           G LNF  FI+ A KHQYK
Sbjct: 541 GALNFLIFIVFAMKHQYK 558

BLAST of CsGy2G025700 vs. TAIR 10
Match: AT1G12110.1 (nitrate transporter 1.1 )

HSP 1 Score: 530.4 bits (1365), Expect = 1.8e-150
Identity = 285/567 (50.26%), Postives = 384/567 (67.72%), Query Frame = 0

Query: 12  EEDDTAVDFW---GNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLT 71
           + DD  +D W   G P D+SKTGGW +A +IL  E  ER+  +GI +NLVTYL G +HL 
Sbjct: 7   KSDDILLDAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMHLG 66

Query: 72  SAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTLATTIPGMR 131
           +A +A  VTNFLG   +L LLGGF+AD  LGRYLT+AIFA+I A GV +LTL+T IPG+R
Sbjct: 67  NATAANTVTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPGLR 126

Query: 132 PPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKEEK 191
           PP C+ +T     C +A+G QL +LY ALY  ALG GG+K++VSGFGSDQFD  +PKE  
Sbjct: 127 PPRCNPTTSS--HCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERS 186

Query: 192 ATIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLLCGTTFYRF 251
              +FFNRF+F I++GSL AV VLVYVQD VGR WGYGI    + +A+ V L GT  YRF
Sbjct: 187 KMTYFFNRFFFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRF 246

Query: 252 KKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQN-----------AKVPYTYRFKC 311
           KK  GSP+T +  V++ AW+ R L  PA PS+L D  +            K+P+T +F+ 
Sbjct: 247 KKLIGSPMTQVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRS 306

Query: 312 LDKAAILDEYA--TTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTV 371
           LDKAAI D+ A  T+   N W +ST+T+VEEVK + +++PIW+T ILFWT+++Q+TT +V
Sbjct: 307 LDKAAIRDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSV 366

Query: 372 EQASFMERKVGSFEIPSGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIG 431
            Q+  ++R +GSFEIP  SM+ F    +LL T++ +++ I + +KL +   GL  LQRIG
Sbjct: 367 AQSETLDRSIGSFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIG 426

Query: 432 IGLVFSIFGMVAAGAVE--KERKASAVGQSTR---ISAFWLIPQFFLVGAGEAFTYVGQL 491
           +GL F    M  A  VE  + R A A G + +   +  + LIPQ+ +VG GEA  Y GQL
Sbjct: 427 LGLFFGSMAMAVAALVELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQL 486

Query: 492 EFFIREAPEQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK--QWLTSNLNKGHLSYF 551
           +FF+RE P+ MK MSTGL LSTL++GFF SS+LVTIV+K T K   W+  +LNKG L  F
Sbjct: 487 DFFLRECPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGKAHPWIADDLNKGRLYNF 546

Query: 552 YWLLAVLGLLNFFFFILLARKHQYKDQ 556
           YWL+AVL  LNF  F++ ++ + YK++
Sbjct: 547 YWLVAVLVALNFLIFLVFSKWYVYKEK 571

BLAST of CsGy2G025700 vs. TAIR 10
Match: AT2G26690.1 (Major facilitator superfamily protein )

HSP 1 Score: 526.9 bits (1356), Expect = 2.0e-149
Identity = 283/546 (51.83%), Postives = 381/546 (69.78%), Query Frame = 0

Query: 17  AVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLTSAKSATIV 76
           AVD+ G P DKSKTGGW+ A LILG E+ ER+  MGI++NLVTYL+  +HL S+ SA IV
Sbjct: 12  AVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETMHLPSSTSANIV 71

Query: 77  TNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTLATTIPGMRPPHCDDST 136
           T+F+G   LL LLGGFLAD+ LGR+ T+ IF++I A+G   L +AT +P +RPP C    
Sbjct: 72  TDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLPELRPPTCHHG- 131

Query: 137 RQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKEEKATIFFFNR 196
                CI A   Q+ +LY +LY IALG GG+KS++SGFGSDQFD  DPKE+    FFFNR
Sbjct: 132 ---EACIPATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAHMAFFFNR 191

Query: 197 FYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLLCGTTFYRFKKPRGSPL 256
           F+F IS+G+L AV VLVY+QD+VGR W YGI   +MA+A+++ LCGT  YR+KK +GSP+
Sbjct: 192 FFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYKKSQGSPV 251

Query: 257 TVIWRVVLLAWKKRSLPHPAHPSFL--NDYQNAKVPYTYRFKCLDKAAILDE------YA 316
             I++V+  A++KR +  P    +L  ++ +  ++ +T +F  LDKAAI+ E        
Sbjct: 252 VQIFQVIAAAFRKRKMELPQSIVYLYEDNPEGIRIEHTDQFHLLDKAAIVAEGDFEQTLD 311

Query: 317 TTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASFMERKVGSF 376
                NPW +S+VT+VEEVKM+ +L+PIW+T I+FWT Y+QM TF+VEQAS M R +GSF
Sbjct: 312 GVAIPNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQASTMRRNIGSF 371

Query: 377 EIPSGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVFSIFGMVAA 436
           +IP+GS++ F   AIL+  ++ ++  +P  +K      G +SLQRI IGLV S  GM AA
Sbjct: 372 KIPAGSLTVFFVAAILITLAVYDRAIMPFWKK-WKGKPGFSSLQRIAIGLVLSTAGMAAA 431

Query: 437 GAVEKERKASAVGQSTR---ISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQMKSMS 496
             VE++R + A   S +   IS F L+PQFFLVGAGEAF Y GQL+FFI ++P+ MK+MS
Sbjct: 432 ALVEQKRLSVAKSSSQKTLPISVFLLVPQFFLVGAGEAFIYTGQLDFFITQSPKGMKTMS 491

Query: 497 TGLFLSTLSMGFFVSSLLVTIVDKVTNKQ----WLTSNLNKGHLSYFYWLLAVLGLLNFF 548
           TGLFL+TLS+GFFVSS LV+IV +VT+      WL  N+N G L YFYWLL +L  +NF 
Sbjct: 492 TGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRLDYFYWLLVILSGINFV 551

BLAST of CsGy2G025700 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 439.1 bits (1128), Expect = 5.6e-123
Identity = 243/560 (43.39%), Postives = 351/560 (62.68%), Query Frame = 0

Query: 15  DTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLTSAKSAT 74
           D  VD   NP +K KTG W A   ILG E  ER+   G+  NLV YL   L+  +A +A 
Sbjct: 10  DGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAAN 69

Query: 75  IVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTLATTIPGMRPPHCDD 134
            VTN+ G   +  L+G F+ADA LGRY T+A F  I   G+ LLTL+ ++PG++P +C+ 
Sbjct: 70  NVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCNA 129

Query: 135 STRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKEEKATIFFF 194
            T         N  Q A+ + ALY IALG GGIK  VS FG+DQFD ND  E+     FF
Sbjct: 130 DT------CHPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFF 189

Query: 195 NRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLLCGTTFYRFKKPRGS 254
           N FYF+I++G+L A  VLV++Q  VG GWG+G+    M +AV     G+ FYR ++P GS
Sbjct: 190 NWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGS 249

Query: 255 PLTVIWRVVLLAWKKRSLPHPAHPSFL--------NDYQNAKVPYTYRFKCLDKAAI--L 314
           PLT I++V++ A++K S+  P   S L        N   + K+ +T   K  DKAA+   
Sbjct: 250 PLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQ 309

Query: 315 DEYATTGPNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASFMERK 374
            +    G  NPW + +VT+VEE+K +  L+P+W+TGI+F T+YSQM+T  V Q + M++ 
Sbjct: 310 SDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQH 369

Query: 375 VG-SFEIPSGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVFSIF 434
           +G +FEIPS S+S F  +++L  T + ++  IP+ARK T N +G T LQR+GIGLV SIF
Sbjct: 370 MGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIF 429

Query: 435 GMVAAGAVEKER-----KASAVGQ-STRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREA 494
            M+ AG +E  R       +A  Q    +S FW IPQ+ L+G  E FT++GQLEFF  +A
Sbjct: 430 AMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQA 489

Query: 495 PEQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQ----WLTSNLNKGHLSYFYWLLA 554
           P+ M+S+ + L L+T+++G ++S++LVT+V K+T K     W+  NLN+GHL YF++LLA
Sbjct: 490 PDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYLLA 549

BLAST of CsGy2G025700 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 416.0 bits (1068), Expect = 5.0e-116
Identity = 234/560 (41.79%), Postives = 343/560 (61.25%), Query Frame = 0

Query: 15  DTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLTSAKSAT 74
           D  +D    P +K+KTG W A   ILGTE  ER+   G+S NL+ YL   +++ +  ++ 
Sbjct: 11  DGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSASK 70

Query: 75  IVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTLATTIPGMRPPHCDD 134
            V+N+ G      L+G F+ADA LGRY T+A F  I   G+ LLT++ ++PG+ P  C  
Sbjct: 71  SVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGLTPT-CSG 130

Query: 135 STRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKEEKATIFFF 194
            T        A  GQ A+ + ALY IALG GGIK  VS FG+DQFD  D KE+++   FF
Sbjct: 131 ET------CHATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFF 190

Query: 195 NRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLLCGTTFYRFKKPRGS 254
           N FYF I++G++ A  VLV++Q  VG GWG G+    MA+AV+    G+ FYR +KP GS
Sbjct: 191 NWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGS 250

Query: 255 PLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNA--------KVPYTYRFKCLDKAAILDE 314
           PLT + +V++ + +K  +  P   S L + Q+A        K+ +T      DKAA+  E
Sbjct: 251 PLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAVETE 310

Query: 315 YATTG--PNNPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASFMERK 374
               G   ++ W + TVT+VEE+K + +L+PIW+TGI+F ++YSQM T  V Q + +++ 
Sbjct: 311 SDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQH 370

Query: 375 VG-SFEIPSGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVFSIF 434
           +G +F+IPS S+S F  +++L    + +KL +P ARK T + +G T LQRIGIGLV SIF
Sbjct: 371 MGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIF 430

Query: 435 GMVAAGAVEKERKASAV------GQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREA 494
            MV+AG +E  R            ++  ++ FW +PQ+FLVG  E FT++GQLEFF  +A
Sbjct: 431 SMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQA 490

Query: 495 PEQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK----QWLTSNLNKGHLSYFYWLLA 554
           P+ M+S+ + L L+ ++ G ++S+ LVT+V KVT       W+  NLN GHL YF+WLLA
Sbjct: 491 PDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFFWLLA 550

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LVE04.6e-23172.58Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1[more]
Q050852.6e-14950.26Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1[more]
Q9SZY42.9e-14851.83Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1[more]
Q9M3907.8e-12243.39Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Q9LFB87.1e-11541.79Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AFR11353.10.099.32nitrate transporter [Cucumis sativus][more]
XP_011649985.10.099.15protein NRT1/ PTR FAMILY 6.4 [Cucumis sativus] >KGN63217.1 hypothetical protein ... [more]
KAA0062862.10.096.94protein NRT1/ PTR FAMILY 6.4 [Cucumis melo var. makuwa][more]
XP_008455932.10.096.94PREDICTED: protein NRT1/ PTR FAMILY 6.4 [Cucumis melo] >TYK09533.1 protein NRT1/... [more]
XP_038901295.10.093.04protein NRT1/ PTR FAMILY 6.4 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
J7LEY90.099.32Nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT1.3 PE=2 SV=1[more]
A0A0A0LQY50.099.15Oligopeptide transporter OS=Cucumis sativus OX=3659 GN=Csa_2G416080 PE=3 SV=1[more]
A0A5A7V3P80.096.94Protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A5D3CCF70.096.94Protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3C2630.096.94protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo OX=3656 GN=LOC103495995 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G21670.13.3e-23272.58Major facilitator superfamily protein [more]
AT1G12110.11.8e-15050.26nitrate transporter 1.1 [more]
AT2G26690.12.0e-14951.83Major facilitator superfamily protein [more]
AT3G54140.15.6e-12343.39peptide transporter 1 [more]
AT5G01180.15.0e-11641.79peptide transporter 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 101..513
e-value: 8.0E-109
score: 364.1
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 14..554
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 7..559
e-value: 2.9E-182
score: 608.5
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 27..554
NoneNo IPR availablePANTHERPTHR11654:SF451PROTEIN NRT1/ PTR FAMILY 6.4coord: 14..554

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy2G025700.1CsGy2G025700.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015706 nitrate transport
biological_process GO:0010167 response to nitrate
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015112 nitrate transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity