CsGy2G014260 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy2G014260
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionL-ascorbate oxidase homolog
LocationGy14Chr2: 14528033 .. 14531347 (-)
RNA-Seq ExpressionCsGy2G014260
SyntenyCsGy2G014260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAGAGGGGAATTTGGTAATGAATTTGAGCTTGGGAATTATTGTACTATTGGTTTGTTGTAGTTTGGGATGGGTGAATGCTGAAGACCCATATAGATATTTCACTTTTCAAGTCACCTACGGTACACGTGCTCCTCTTGGGTTTAATCAAAAGGTTTTACCTCTCCTCTAATTTATGGTTCATGACACAAATTTCAACCTCATTTGTGATCTACAGTTCTTTTTCAAAATTTATACCTCATTTCAAATTATATAATTTTGAAAACACTTGTAAAAGTTAAAATGAAATGAATAACACGATGAAGAGAAACCATTGTTGCACTTTTATACGGTCACGTTTCAAGTTTCAAGTTCACAATCATCTTTGTTAATTCTTTTTTGTGTTTAAGTTAAGTTCTAGCTCCATTCATGTTTCATCGTATAATGTAAGTTTGTTAGGTTGATTTTATTAACGATAAGATATTTTCATGTGATGTCTATAGTATAGTATGCATTATTAAAAATGAAGAGTAATTTGGTTGGACATGTGTATTGAACGACCCAATTTCAATTCCACACTTTTTGTGCTCTTAACTCATTTCTTTTTTACCAAGACGTAGCCATTTTTTTTAAAAAAAGTAATTAATTTGTAACTAATTAAACAACCTTTAAACCCTAATTAACTTAACTAAAACGGGTGTTTCAAGTAAGAAAAGTATTGCCCTCTAATAATAACAACAACTGTATTTGGAAAGATTTTAACCAGATTGTAGGAACTAAAGTGAAAGCAAGTGCAAAATATAGGGAAAATAAAGAATAAATTAACTTCAAGAACTGCATTTTTTTTTTCAATTTTGTAATTAAAGATGAAAAAGTTTATGCAACAAAAAAATCAAAATTTAATATTGTGTTATAATTTTTTGCATAGGTAATCCTTGTGAATGGGCAATTTCCAGCCCCAAAGATAGAGTGTGTGACAAATGACAACATAATTGTGAATGTCATTAACAAGCTTGATGAGCCTTTTCTCTTTACATGGTGAGTCCTCAAACTTAATTATTAATTATAAATCAAGATAAGACCCTTATAAATGTAATGCAAAAGAATTTTCATATATAATTGATCCTATGAAAGTGTATACATATTTAATTAATTTGTCATTGGAATTATGTGAATAAATTTAGGAGTGGAGTGAAGCAAAGGAGGACATCATGGCAAGATGGGGTGTTAGGGACAAATTGCCCAATCCCTCCAAACTCAAATTGGACATACAAATTCCAAGTTAAAGATCAAATTGGAACTTTCACATACTTCCCTTCCATCAACTTCCAAAGGGCTTCTGGTGGCTTTGGAGCCTTTGTAATTCAACCTAGAAGTGTCATAACAACTCCCTACCCCATTCCCGCTGCCGAGGTCGACCTTCTCATCAGCGACTGGTACAATACCGACCATAAGGTAATTTCTACTTTCTTCTCATTCCTACTATACATTATATGTCTACTAGCTAGTAGTAAATCGTAATTCAAACCTTTACATAATTTTAAGCTACATTAGAAGTACTCATTAATGGTATGTCATTTTGTGCAGACGTTGCGTAAGAAGTTGGATTCGGGAATTTTGCTGCCTTTACCTAATCGTCTTCTTATCAATGGACAACCTAGTTTAACACTAACTCTTAAAAAAGGTAACAATATAATATTCACTTCGTTATATTGTTAACTATATATGATCGATATAAGTACATATGTAGTAATTAAATTATATATTGTTTCTGTATAGGGCTAACATACAAGTTGAGGATATCAAATGTTGGGATAGCAACATCGATAAACTTTAGGATTCAAGGACATTTAATGACACTTGTGGAAGTAGAAGGAGCTCACACAGTTCAAGAAGTTTACGAATCATTGGATATTCATCCTGGCCAATCTGCTGCTGTTTTGGTGTCTCTTAACAATTTACCAAAGGATTATTACTTTGTGGTTTCTTCACGTTTCACTAAGCCTACTATTCTTACTACTGTTGGTATCCTTAGATATGAAGGCTCTTCCATTCCTCCTTCCAAGCCTTTGCCCATTGGACCTACTTATCAACTCCATTGGTCCATGAAGCAAGCCCGAACCATCAGGTTCGTTCTGTTTGACACTTCATGTTTCGTTTGCTTTTAAAGATTAATCTGCTATTCTTTTCATATTTTCTATTTAAACGTCTTAACAGAATCACCTTGGTCTTTTAAAATACAAATAAATCAGTTGATAGATAAGTAGTACTCATACCAACTATATTTTCGATCACGAGACCATATTGTTAGTTGCTATAAAACATACCGTTCTTATACCTATAAAGAAAAGATGAATGGAGAGTGATTATACAACCACAATAAATTCATACAACGTCCTAATGATCAGTTGAGTTATACTTCAAATGATTTCGACAGATTAAACTTGACAGCAAATGCAGCAAGGCCGAACCCACAAGGATCATTCCACTATGGAAACATAACAGTAGTGAGAAGACTCATATTAGAAAATTCATTGAGAAAGATAAAAGGAGCTCTTCGTTGTGCAGTGAACAGTGTGTCATATGTTGATCCACCAACACCTTTAAAGTTGGCTGATTGGTTCAAAATTGATGGTGTTTTTGACTTCAAAACCTTCACTGATGTCCCAACTTCAAGACCTGCAATGCTTGGAGTCTCTGTCGTTAACATTACCCTTCATGATTTTGTGGAAATTGTGTTCCAGAACAATGAAAACACTGTCCAAGCTTGGCATTTTGATGGAACAAGTTTCTATGTTGTTGGGTTAGCAATTCCTTTCATAACCCTTTTTCGTTTTTGTAGGATTTTTATTGGTTTTCTAGACTAAATCTTAGTGTTTTTTGTTCTGTTTTTGAACAGATATGGTTCTGGTGTTTGGAGTTATAAAATGAGGAAACGTTATAACTTAGTGGATGGTATATCAAGACACACTGTTCAGGTACTTTTGTTGACTTCCATTTGTTATCTATGTCGAATGCAAGTAACAATCCTAGTTAATATATTTTTCATCCAATGAATATATATAATCATATAGAATATTGTAAAATCAAATTTAAAAAAGAAAAAAAGATTGATTGTGTGTGTGTTTTTGGGCAGGTATATCCAAGGTCATGGACAGCAATATTAGTGTCATTAGACAACAAGGGGATGTGGAACTTAAGATCTACAATATGGTGGAGAAATTATTTGGGTCAACAGTTATATGTCAGAGTTTGGAATGATGAAAAGAGTTTATATACTGAGACTGATATTCCACCCAATGCTCTCAAGTGTGGCAAAGCATCCAAGTATTAA

mRNA sequence

ATGAGAGAGGGGAATTTGGTAATGAATTTGAGCTTGGGAATTATTGTACTATTGGTTTGTTGTAGTTTGGGATGGGTGAATGCTGAAGACCCATATAGATATTTCACTTTTCAAGTCACCTACGGTACACGTGCTCCTCTTGGGTTTAATCAAAAGGTAATCCTTGTGAATGGGCAATTTCCAGCCCCAAAGATAGAGTGTGTGACAAATGACAACATAATTGTGAATGTCATTAACAAGCTTGATGAGCCTTTTCTCTTTACATGGAGTGGAGTGAAGCAAAGGAGGACATCATGGCAAGATGGGGTGTTAGGGACAAATTGCCCAATCCCTCCAAACTCAAATTGGACATACAAATTCCAAGTTAAAGATCAAATTGGAACTTTCACATACTTCCCTTCCATCAACTTCCAAAGGGCTTCTGGTGGCTTTGGAGCCTTTGTAATTCAACCTAGAAGTGTCATAACAACTCCCTACCCCATTCCCGCTGCCGAGGTCGACCTTCTCATCAGCGACTGGTACAATACCGACCATAAGACGTTGCGTAAGAAGTTGGATTCGGGAATTTTGCTGCCTTTACCTAATCGTCTTCTTATCAATGGACAACCTAGTTTAACACTAACTCTTAAAAAAGGGCTAACATACAAGTTGAGGATATCAAATGTTGGGATAGCAACATCGATAAACTTTAGGATTCAAGGACATTTAATGACACTTGTGGAAGTAGAAGGAGCTCACACAGTTCAAGAAGTTTACGAATCATTGGATATTCATCCTGGCCAATCTGCTGCTGTTTTGGTGTCTCTTAACAATTTACCAAAGGATTATTACTTTGTGGTTTCTTCACGTTTCACTAAGCCTACTATTCTTACTACTGTTGGTATCCTTAGATATGAAGGCTCTTCCATTCCTCCTTCCAAGCCTTTGCCCATTGGACCTACTTATCAACTCCATTGGTCCATGAAGCAAGCCCGAACCATCAGATTAAACTTGACAGCAAATGCAGCAAGGCCGAACCCACAAGGATCATTCCACTATGGAAACATAACAGTAGTGAGAAGACTCATATTAGAAAATTCATTGAGAAAGATAAAAGGAGCTCTTCGTTGTGCAGTGAACAGTGTGTCATATGTTGATCCACCAACACCTTTAAAGTTGGCTGATTGGTTCAAAATTGATGGTGTTTTTGACTTCAAAACCTTCACTGATGTCCCAACTTCAAGACCTGCAATGCTTGGAGTCTCTGTCGTTAACATTACCCTTCATGATTTTGTGGAAATTGTGTTCCAGAACAATGAAAACACTGTCCAAGCTTGGCATTTTGATGGAACAAGTTTCTATGTTGTTGGATATGGTTCTGGTGTTTGGAGTTATAAAATGAGGAAACGTTATAACTTAGTGGATGGTATATCAAGACACACTGTTCAGGTATATCCAAGGTCATGGACAGCAATATTAGTGTCATTAGACAACAAGGGGATGTGGAACTTAAGATCTACAATATGGTGGAGAAATTATTTGGGTCAACAGTTATATGTCAGAGTTTGGAATGATGAAAAGAGTTTATATACTGAGACTGATATTCCACCCAATGCTCTCAAGTGTGGCAAAGCATCCAAGTATTAA

Coding sequence (CDS)

ATGAGAGAGGGGAATTTGGTAATGAATTTGAGCTTGGGAATTATTGTACTATTGGTTTGTTGTAGTTTGGGATGGGTGAATGCTGAAGACCCATATAGATATTTCACTTTTCAAGTCACCTACGGTACACGTGCTCCTCTTGGGTTTAATCAAAAGGTAATCCTTGTGAATGGGCAATTTCCAGCCCCAAAGATAGAGTGTGTGACAAATGACAACATAATTGTGAATGTCATTAACAAGCTTGATGAGCCTTTTCTCTTTACATGGAGTGGAGTGAAGCAAAGGAGGACATCATGGCAAGATGGGGTGTTAGGGACAAATTGCCCAATCCCTCCAAACTCAAATTGGACATACAAATTCCAAGTTAAAGATCAAATTGGAACTTTCACATACTTCCCTTCCATCAACTTCCAAAGGGCTTCTGGTGGCTTTGGAGCCTTTGTAATTCAACCTAGAAGTGTCATAACAACTCCCTACCCCATTCCCGCTGCCGAGGTCGACCTTCTCATCAGCGACTGGTACAATACCGACCATAAGACGTTGCGTAAGAAGTTGGATTCGGGAATTTTGCTGCCTTTACCTAATCGTCTTCTTATCAATGGACAACCTAGTTTAACACTAACTCTTAAAAAAGGGCTAACATACAAGTTGAGGATATCAAATGTTGGGATAGCAACATCGATAAACTTTAGGATTCAAGGACATTTAATGACACTTGTGGAAGTAGAAGGAGCTCACACAGTTCAAGAAGTTTACGAATCATTGGATATTCATCCTGGCCAATCTGCTGCTGTTTTGGTGTCTCTTAACAATTTACCAAAGGATTATTACTTTGTGGTTTCTTCACGTTTCACTAAGCCTACTATTCTTACTACTGTTGGTATCCTTAGATATGAAGGCTCTTCCATTCCTCCTTCCAAGCCTTTGCCCATTGGACCTACTTATCAACTCCATTGGTCCATGAAGCAAGCCCGAACCATCAGATTAAACTTGACAGCAAATGCAGCAAGGCCGAACCCACAAGGATCATTCCACTATGGAAACATAACAGTAGTGAGAAGACTCATATTAGAAAATTCATTGAGAAAGATAAAAGGAGCTCTTCGTTGTGCAGTGAACAGTGTGTCATATGTTGATCCACCAACACCTTTAAAGTTGGCTGATTGGTTCAAAATTGATGGTGTTTTTGACTTCAAAACCTTCACTGATGTCCCAACTTCAAGACCTGCAATGCTTGGAGTCTCTGTCGTTAACATTACCCTTCATGATTTTGTGGAAATTGTGTTCCAGAACAATGAAAACACTGTCCAAGCTTGGCATTTTGATGGAACAAGTTTCTATGTTGTTGGATATGGTTCTGGTGTTTGGAGTTATAAAATGAGGAAACGTTATAACTTAGTGGATGGTATATCAAGACACACTGTTCAGGTATATCCAAGGTCATGGACAGCAATATTAGTGTCATTAGACAACAAGGGGATGTGGAACTTAAGATCTACAATATGGTGGAGAAATTATTTGGGTCAACAGTTATATGTCAGAGTTTGGAATGATGAAAAGAGTTTATATACTGAGACTGATATTCCACCCAATGCTCTCAAGTGTGGCAAAGCATCCAAGTATTAA

Protein sequence

MREGNLVMNLSLGIIVLLVCCSLGWVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNGQFPAPKIECVTNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDHKTLRKKLDSGILLPLPNRLLINGQPSLTLTLKKGLTYKLRISNVGIATSINFRIQGHLMTLVEVEGAHTVQEVYESLDIHPGQSAAVLVSLNNLPKDYYFVVSSRFTKPTILTTVGILRYEGSSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENSLRKIKGALRCAVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVVNITLHDFVEIVFQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVYPRSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCGKASKY*
Homology
BLAST of CsGy2G014260 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 2.9e-155
Identity = 272/540 (50.37%), Postives = 357/540 (66.11%), Query Frame = 0

Query: 13  GIIVLLVCCSLG-----WVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNGQFPAPKIEC 72
           G+ +L  C  L       V+AEDPY +  + VTYGT +PLG  Q+VIL+NGQFP P I  
Sbjct: 3   GVKLLAACLYLAAAATVVVHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINS 62

Query: 73  VTNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIG 132
            +N+N+I+NV N LDEPFL TW+G++ R+  WQDG  GT CPI P +N+TY FQ KDQIG
Sbjct: 63  TSNNNVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIG 122

Query: 133 TFTYFPSINFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDHKTLRKKLDS 192
           ++ Y+P+    RA+GG+G   +  R +I  PY  P  +  +LI DWY   H  L+K LD 
Sbjct: 123 SYFYYPTTGMHRAAGGYGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDG 182

Query: 193 GILLPLPNRLLINGQ-------PSLTLTLKKGLTYKLRISNVGIATSINFRIQGHLMTLV 252
           G  +  P+ ++ING+        +   TLK G TY++RI NVG+ TSINFRIQ H M LV
Sbjct: 183 GRTIGRPDGIVINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMKLV 242

Query: 253 EVEGAHTVQEVYESLDIHPGQSAAVLVSLNNLPKDYYFVVSSRFTKPTILTTVGILRYEG 312
           E+EG+H +Q  Y+SLD+H GQ    +V+ N  PKDYY V SSRF K T++TT G+LRYEG
Sbjct: 243 EMEGSHVLQNDYDSLDVHVGQCFGTIVTANQEPKDYYMVASSRFLK-TVITTTGLLRYEG 302

Query: 313 SSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENS 372
              P S  LP GP     WS+ Q R+ R NLTA+AARPNPQGS+HYG I + R + L N+
Sbjct: 303 GKGPASSQLPAGPV-GWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNT 362

Query: 373 LRKIKGALRCAVNSVSYVDPPTPLKLADWFKI-DGVFDFKTFTDVPTS---RPAMLGVSV 432
             K+ G LR A+N VS+ +P TPLKLA++F I D VF + T TD PT    +   +  +V
Sbjct: 363 QGKVDGKLRFALNGVSHTEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNV 422

Query: 433 VNITLHDFVEIVFQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQ 492
           +NIT   FVE+VF+N+E +VQ+WH DG SF+ V    G W+ + RK YNL+D +SRHTVQ
Sbjct: 423 LNITHRTFVEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQ 482

Query: 493 VYPRSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCG 537
           VYP+ W AIL++ DN GMWN+RS    R YLGQQLY  V + EKSL  E ++P  +L+CG
Sbjct: 483 VYPKCWAAILLTFDNCGMWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCG 540

BLAST of CsGy2G014260 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 8.4e-155
Identity = 265/535 (49.53%), Postives = 361/535 (67.48%), Query Frame = 0

Query: 15  IVLLVCCSLGWVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNGQFPAPKIECVTNDNII 74
           + LL+C S+G V AEDPY YF + VTYGT APLG  Q+ IL+NGQFP P+I C +N+NI+
Sbjct: 9   VALLLCLSVG-VIAEDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINCTSNNNIV 68

Query: 75  VNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGTFTYFPS 134
           VNV N LDEPFLFTW+GV+ R+ SWQDG  GT CPI P  N+TY+FQVKDQIG+++YFP+
Sbjct: 69  VNVFNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQIGSYSYFPT 128

Query: 135 INFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDHKTLRKKLDSGILLPLP 194
               RA+GG+GA  +  R++I  P+  PA E ++ + DWYN  HKTL+K LD G  +  P
Sbjct: 129 TALHRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDGGRTIGRP 188

Query: 195 NRLLINGQPSLT-------LTLKKGLTYKLRISNVGIATSINFRIQGHLMTLVEVEGAHT 254
           + ++ING+ +          T++ G TY+ R  N+G+ +S+N R QGH M LVE+EG+HT
Sbjct: 189 DGIIINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLVELEGSHT 248

Query: 255 VQEVYESLDIHPGQSAAVLVSLNNLPKDYYFVVSSRFTKPTILTTVGILRYEGSSIPPSK 314
           VQ +Y+SLD+H GQ  +VLV+ +  PKDYY VVSSRF K   L++V I+RY     P S 
Sbjct: 249 VQNIYDSLDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQA-LSSVAIIRYANGKGPASP 308

Query: 315 PLPIGP---TYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENSLRKI 374
            LP  P   T  + WSM Q R+ R NLTA+AARPNPQGS+HYG I + R + + NS+ ++
Sbjct: 309 ELPTPPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTIKIFNSMSQV 368

Query: 375 KGALRCAVNSVSYVDPPTPLKLADWF-KIDGVFDFKTFTDVPTSRPAMLGV--SVVNITL 434
            G LR  +N +S+ +  TPLKL ++F   +  F +    D   + P+ L +  +V N T 
Sbjct: 369 GGKLRYGLNGISHTNGETPLKLVEYFGATNKAFKYDLMADEAPADPSKLTIATNVKNATY 428

Query: 435 HDFVEIVFQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVYPRS 494
            +FVEI+F+N+E T++ +H DG SF+ V    G WS + RK YNLVDG+SR+ +QVYP S
Sbjct: 429 RNFVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLSRNNIQVYPNS 488

Query: 495 WTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCG 537
           W AI+++ DN GMWNLRS +W + YLG+QLY  V +  +SL  E +IP N   CG
Sbjct: 489 WAAIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPDNHPLCG 541

BLAST of CsGy2G014260 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 1.9e-146
Identity = 265/548 (48.36%), Postives = 358/548 (65.33%), Query Frame = 0

Query: 14  IIVLLVCCSLGWVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNGQFPAPKIECVTNDNI 73
           I++L+   ++ +  A DPY ++ F+V+Y T +PLG  Q+VI +NG+FP P I   TN+N+
Sbjct: 6   ILLLVFFVNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTNENL 65

Query: 74  IVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGTFTYFP 133
           +VNV NKLDE  L  W+G++QRR SWQDGVLGTNCPIPP  NWTY+FQVKDQIG+F YFP
Sbjct: 66  VVNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFFYFP 125

Query: 134 SINFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDHKTLRKKLDSGILLPL 193
           S++FQRASGGFG+FV+ PR++I  P+  P  ++ + I DWY  +H  LRK LD G  L +
Sbjct: 126 SLHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKDLGM 185

Query: 194 PNRLLINGQPSL--------------TLTLKKGLTYKLRISNVGIATSINFRIQGHLMTL 253
           P+ +LING+                 T+T+  G TY+LR+SNVGI+TS+NFRIQGH + L
Sbjct: 186 PDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHNLVL 245

Query: 254 VEVEGAHTVQEVYESLDIHPGQSAAVLVSLN-NLPKDYYFVVSSRFTKPTI---LTTVGI 313
            E EG++TVQ+ Y SLDIH GQS + LV+++ N   DYY V S+R    TI   +T VGI
Sbjct: 246 AESEGSYTVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTGVGI 305

Query: 314 LRYEGSSIPPSKPLPIGP--TYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVR 373
           L+Y  S       LP GP   +   +SM QAR+IR N++A+ ARPNPQGSF YG+I V  
Sbjct: 306 LKYTNSKGKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSINVTD 365

Query: 374 RLILENSLR-KIKGALRCAVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVPTSRPAML 433
             +L N     I G  R  +N +S+ +P TP++LAD  K+  V+    F   P + PA +
Sbjct: 366 VYVLRNMPPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKDVYKL-DFPKRPLTGPAKV 425

Query: 434 GVSVVNITLHDFVEIVFQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISR 493
             S++N T   F+E+V QNN+  +Q++H  G +F+VVG   G W+   R  YN  DGI+R
Sbjct: 426 ATSIINGTYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYNKWDGIAR 485

Query: 494 HTVQVYPRSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNA 541
            T+QVYP +W+AIL+SLDN G WNLR+      YLGQ+ YVRV N +++  TE   P N 
Sbjct: 486 STIQVYPGAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTEFGHPDNV 545

BLAST of CsGy2G014260 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 1.0e-144
Identity = 261/529 (49.34%), Postives = 342/529 (64.65%), Query Frame = 0

Query: 28  AEDPYRYFTFQVTYGTRAPLGFNQKVILVNGQFPAPKIECVTNDNIIVNVINKLDEPFLF 87
           A DP+  + F+V+Y T +PLG  Q+VI VNGQFP P +   TN N++VNV N LDEP L 
Sbjct: 24  AADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNATTNYNVVVNVFNHLDEPLLL 83

Query: 88  TWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGTFTYFPSINFQRASGGFGAF 147
           TW G++ RR SWQDGVLGTNCPIPP  N+TY+FQVKDQIG+F Y PS+NFQRASGGFG  
Sbjct: 84  TWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGSFFYSPSLNFQRASGGFGPI 143

Query: 148 VIQPRSVITTPYPIPAAEVDLLISDWYNTDHKTLRKKLDSGILLPLPNRLLINGQ----- 207
           VI  R +I  P+P P  E+  +I DWY  DHK LR+ LDSG  L +P+ +LING+     
Sbjct: 144 VINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSGKELGMPDGVLINGKGPYKY 203

Query: 208 --------PSLTLTLKKGLTYKLRISNVGIATSINFRIQGHLMTLVEVEGAHTVQEVYES 267
                     LT  ++ G TY++R+ NVGI+TS+NFRIQ H + LVE EG +T Q  +  
Sbjct: 204 NSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNHSLLLVETEGHYTSQANFTD 263

Query: 268 LDIHPGQSAAVLVSLN-NLPKDYYFVVSSRFTKPTI---LTTVGILRYEGSSIPPSKPLP 327
            D+H GQS + LV+++ +   DYY V S+RF   T+   +T V IL Y  S  P S PLP
Sbjct: 264 FDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRVTGVAILHYSNSKGPVSGPLP 323

Query: 328 IGPT-YQLHWS-MKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILEN-SLRKIKGA 387
           +  T     WS M Q +TIR N +A+ ARPNPQGSFHYG I +    IL +     I GA
Sbjct: 324 VPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQINITNTYILRSLPPTIINGA 383

Query: 388 LRCAVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVVNITLHDFVEI 447
           LR  +N +S+V+P TP++LAD  K+ G +    F D P +RP  L  S++N T   F+++
Sbjct: 384 LRATLNGISFVNPSTPVRLADRNKVKGAYKL-DFPDRPFNRPLRLDRSMINATYKGFIQV 443

Query: 448 VFQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVYPRSWTAILV 507
           VFQNN+  +Q++H DG SF+VVG   G+WS   +  YN  D ISR T++VYP  WTA+L+
Sbjct: 444 VFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGSYNNWDAISRSTIEVYPGGWTAVLI 503

Query: 508 SLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCG 537
           SLDN G+WN+R     R YLG++ Y+R+ N E+   TE D P N L CG
Sbjct: 504 SLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGKTEMDPPDNVLYCG 551

BLAST of CsGy2G014260 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 1.2e-140
Identity = 254/534 (47.57%), Postives = 341/534 (63.86%), Query Frame = 0

Query: 24  GWVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNGQFPAPKIECVTNDNIIVNVINKLDE 83
           G+  A DPY  + F ++Y T +PLG  Q+VI VNG+FP P I   TN N+ VNV+N LDE
Sbjct: 19  GFSFAGDPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYNVHVNVLNHLDE 78

Query: 84  PFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGTFTYFPSINFQRASGG 143
           P L TW GV+ RR SWQDGVLGTNCPIPPN N+TY FQ+KDQIG++ Y PS+NFQRASGG
Sbjct: 79  PLLLTWPGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSPSLNFQRASGG 138

Query: 144 FGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDHKTLRKKLDSGILLPLPNRLLINGQP 203
           FGA +I  R ++  P+  P  E+  +I DWY  +H  LR+ LDSG  L +P+ +LING+ 
Sbjct: 139 FGALIINNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGMPDGVLINGKG 198

Query: 204 SL-------------TLTLKKGLTYKLRISNVGIATSINFRIQGHLMTLVEVEGAHTVQE 263
                          T+ +  G TY++R+ NVGI+TS+NFRIQ H + L+E EG +T Q 
Sbjct: 199 PFKYNSSVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLIETEGRYTSQM 258

Query: 264 VYESLDIHPGQSAAVLVSLN-NLPKDYYFVVSSRFTKPTI---LTTVGILRYEGSSIPPS 323
            +   D+H GQS + LV+++ N   DYY V S+RF   T+   +T VGIL Y  S  P S
Sbjct: 259 NFTDFDVHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGILHYSNSKGPAS 318

Query: 324 KPLPIGPTYQLH-WS-MKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILEN-SLRK 383
            PLP+  T   H WS M Q R I+ N +A+ ARPNPQGSFHYG I + R  IL +    K
Sbjct: 319 GPLPVSATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHYGQINITRTYILRSLPPTK 378

Query: 384 IKGALRCAVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVP-TSRPAMLGVSVVNITLH 443
           I G LR  +N +S+V+P TP++LAD  K+ G +    F D P   +   L  S++N T  
Sbjct: 379 INGKLRATLNGISFVNPSTPMRLADDHKVKGDY-MLDFPDRPLDEKLPRLSSSIINATYK 438

Query: 444 DFVEIVFQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVYPRSW 503
            F++++FQNN+  +Q++H DG +FYVV    G+WS      YN  D ++R TV+VYP +W
Sbjct: 439 GFIQVIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPGAW 498

Query: 504 TAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCG 537
           TA+L+SLDN G+WN+R     R YLGQ+ Y+R+ N E++  TE D P N + CG
Sbjct: 499 TAVLISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCG 551

BLAST of CsGy2G014260 vs. NCBI nr
Match: XP_004148037.1 (L-ascorbate oxidase homolog [Cucumis sativus])

HSP 1 Score: 1113 bits (2880), Expect = 0.0
Identity = 541/541 (100.00%), Postives = 541/541 (100.00%), Query Frame = 0

Query: 1   MREGNLVMNLSLGIIVLLVCCSLGWVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNGQF 60
           MREGNLVMNLSLGIIVLLVCCSLGWVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNGQF
Sbjct: 1   MREGNLVMNLSLGIIVLLVCCSLGWVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNGQF 60

Query: 61  PAPKIECVTNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKF 120
           PAPKIECVTNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKF
Sbjct: 61  PAPKIECVTNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKF 120

Query: 121 QVKDQIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDHKT 180
           QVKDQIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDHKT
Sbjct: 121 QVKDQIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDHKT 180

Query: 181 LRKKLDSGILLPLPNRLLINGQPSLTLTLKKGLTYKLRISNVGIATSINFRIQGHLMTLV 240
           LRKKLDSGILLPLPNRLLINGQPSLTLTLKKGLTYKLRISNVGIATSINFRIQGHLMTLV
Sbjct: 181 LRKKLDSGILLPLPNRLLINGQPSLTLTLKKGLTYKLRISNVGIATSINFRIQGHLMTLV 240

Query: 241 EVEGAHTVQEVYESLDIHPGQSAAVLVSLNNLPKDYYFVVSSRFTKPTILTTVGILRYEG 300
           EVEGAHTVQEVYESLDIHPGQSAAVLVSLNNLPKDYYFVVSSRFTKPTILTTVGILRYEG
Sbjct: 241 EVEGAHTVQEVYESLDIHPGQSAAVLVSLNNLPKDYYFVVSSRFTKPTILTTVGILRYEG 300

Query: 301 SSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENS 360
           SSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENS
Sbjct: 301 SSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENS 360

Query: 361 LRKIKGALRCAVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVVNIT 420
           LRKIKGALRCAVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVVNIT
Sbjct: 361 LRKIKGALRCAVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVVNIT 420

Query: 421 LHDFVEIVFQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVYPR 480
           LHDFVEIVFQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVYPR
Sbjct: 421 LHDFVEIVFQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVYPR 480

Query: 481 SWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCGKASK 540
           SWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCGKASK
Sbjct: 481 SWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCGKASK 540

BLAST of CsGy2G014260 vs. NCBI nr
Match: KAE8651923.1 (hypothetical protein Csa_006507 [Cucumis sativus])

HSP 1 Score: 1111 bits (2873), Expect = 0.0
Identity = 540/540 (100.00%), Postives = 540/540 (100.00%), Query Frame = 0

Query: 1   MREGNLVMNLSLGIIVLLVCCSLGWVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNGQF 60
           MREGNLVMNLSLGIIVLLVCCSLGWVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNGQF
Sbjct: 1   MREGNLVMNLSLGIIVLLVCCSLGWVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNGQF 60

Query: 61  PAPKIECVTNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKF 120
           PAPKIECVTNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKF
Sbjct: 61  PAPKIECVTNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKF 120

Query: 121 QVKDQIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDHKT 180
           QVKDQIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDHKT
Sbjct: 121 QVKDQIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDHKT 180

Query: 181 LRKKLDSGILLPLPNRLLINGQPSLTLTLKKGLTYKLRISNVGIATSINFRIQGHLMTLV 240
           LRKKLDSGILLPLPNRLLINGQPSLTLTLKKGLTYKLRISNVGIATSINFRIQGHLMTLV
Sbjct: 181 LRKKLDSGILLPLPNRLLINGQPSLTLTLKKGLTYKLRISNVGIATSINFRIQGHLMTLV 240

Query: 241 EVEGAHTVQEVYESLDIHPGQSAAVLVSLNNLPKDYYFVVSSRFTKPTILTTVGILRYEG 300
           EVEGAHTVQEVYESLDIHPGQSAAVLVSLNNLPKDYYFVVSSRFTKPTILTTVGILRYEG
Sbjct: 241 EVEGAHTVQEVYESLDIHPGQSAAVLVSLNNLPKDYYFVVSSRFTKPTILTTVGILRYEG 300

Query: 301 SSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENS 360
           SSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENS
Sbjct: 301 SSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENS 360

Query: 361 LRKIKGALRCAVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVVNIT 420
           LRKIKGALRCAVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVVNIT
Sbjct: 361 LRKIKGALRCAVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVVNIT 420

Query: 421 LHDFVEIVFQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVYPR 480
           LHDFVEIVFQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVYPR
Sbjct: 421 LHDFVEIVFQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVYPR 480

Query: 481 SWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCGKASK 540
           SWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCGKASK
Sbjct: 481 SWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCGKASK 540

BLAST of CsGy2G014260 vs. NCBI nr
Match: XP_008457817.1 (PREDICTED: L-ascorbate oxidase homolog [Cucumis melo])

HSP 1 Score: 1042 bits (2694), Expect = 0.0
Identity = 504/541 (93.16%), Postives = 521/541 (96.30%), Query Frame = 0

Query: 1   MREGNLVMNLSLGIIVLLVCCSLGWVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNGQF 60
           MREG ++ NLSLGI+++ VCCSLGWVN EDPYRY+TFQVTYGTRAPLG  QKVILVNGQF
Sbjct: 1   MREGKMI-NLSLGIMIIWVCCSLGWVNGEDPYRYYTFQVTYGTRAPLGVAQKVILVNGQF 60

Query: 61  PAPKIECVTNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKF 120
           PAPKIEC TNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKF
Sbjct: 61  PAPKIECATNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKF 120

Query: 121 QVKDQIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDHKT 180
           QVKDQIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYP+P AE+DLLI DWY+TDHKT
Sbjct: 121 QVKDQIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYPLPHAEIDLLIGDWYDTDHKT 180

Query: 181 LRKKLDSGILLPLPNRLLINGQPSLTLTLKKGLTYKLRISNVGIATSINFRIQGHLMTLV 240
           LRK+LDSG+LLPLP RLLINGQPS TL LKKGLTYKLRISNVGIATSINFRIQGHLMTLV
Sbjct: 181 LRKQLDSGVLLPLPKRLLINGQPSSTLILKKGLTYKLRISNVGIATSINFRIQGHLMTLV 240

Query: 241 EVEGAHTVQEVYESLDIHPGQSAAVLVSLNNLPKDYYFVVSSRFTKPTILTTVGILRYEG 300
           EVEGAHTVQ+VYESLDIHPGQSAAVLVSLN LPKDYYFVVSSRFTKP ILTTVGILRYEG
Sbjct: 241 EVEGAHTVQDVYESLDIHPGQSAAVLVSLNRLPKDYYFVVSSRFTKP-ILTTVGILRYEG 300

Query: 301 SSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENS 360
           SSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLIL+NS
Sbjct: 301 SSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILKNS 360

Query: 361 LRKIKGALRCAVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVVNIT 420
           L KIKGA+RCAVN+VSYVDPPTPLKLADWFKI GVFDFKTFTD+PT RPAMLGVSVVNIT
Sbjct: 361 LTKIKGAVRCAVNNVSYVDPPTPLKLADWFKIGGVFDFKTFTDIPTPRPAMLGVSVVNIT 420

Query: 421 LHDFVEIVFQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVYPR 480
           LHDFVEIVFQN+ENTVQAWHFDGTSFYVVGYGSG WSYKMRKRYNLVDGISRHTVQVYPR
Sbjct: 421 LHDFVEIVFQNDENTVQAWHFDGTSFYVVGYGSGDWSYKMRKRYNLVDGISRHTVQVYPR 480

Query: 481 SWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCGKASK 540
           SWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTE DIP NALKCGKASK
Sbjct: 481 SWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTEADIPLNALKCGKASK 539

BLAST of CsGy2G014260 vs. NCBI nr
Match: XP_038902225.1 (L-ascorbate oxidase homolog [Benincasa hispida])

HSP 1 Score: 1038 bits (2684), Expect = 0.0
Identity = 506/543 (93.19%), Postives = 519/543 (95.58%), Query Frame = 0

Query: 1   MREGNLVMNLSLGIIVLLVCCSLG--WVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNG 60
           M+EGNLV NL+LGII  L+C  LG  WVN EDPYRY+TFQVTYGTRAPLG  QKVILVNG
Sbjct: 1   MKEGNLV-NLTLGII--LICYGLGGGWVNGEDPYRYYTFQVTYGTRAPLGVKQKVILVNG 60

Query: 61  QFPAPKIECVTNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTY 120
           QFPAPKIECVTNDNIIVNVINKLDEPFLFTWSG+KQRRTSWQDGVLGTNCPIPPNSNWTY
Sbjct: 61  QFPAPKIECVTNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTY 120

Query: 121 KFQVKDQIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDH 180
           KFQVKDQIGTFTYFPSINFQRA+GGFGAFVIQPRSVI TPYPIPAAE+DLLI DWY+TDH
Sbjct: 121 KFQVKDQIGTFTYFPSINFQRAAGGFGAFVIQPRSVIATPYPIPAAELDLLIGDWYDTDH 180

Query: 181 KTLRKKLDSGILLPLPNRLLINGQPSLTLTLKKGLTYKLRISNVGIATSINFRIQGHLMT 240
           KTLRKKLDSGI+LPLPNRLLINGQPS TL LKKGLTYKLRISNVGIATSINFRIQGHLMT
Sbjct: 181 KTLRKKLDSGIMLPLPNRLLINGQPSTTLILKKGLTYKLRISNVGIATSINFRIQGHLMT 240

Query: 241 LVEVEGAHTVQEVYESLDIHPGQSAAVLVSLNNLPKDYYFVVSSRFTKPTILTTVGILRY 300
           LVEVEG HTVQE YESLDIHPGQSAAVLVSLNNLPKDYYFV SSRFTKP ILTT+GILRY
Sbjct: 241 LVEVEGGHTVQEAYESLDIHPGQSAAVLVSLNNLPKDYYFVASSRFTKP-ILTTIGILRY 300

Query: 301 EGSSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILE 360
           EGSSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILE
Sbjct: 301 EGSSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILE 360

Query: 361 NSLRKIKGALRCAVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVVN 420
           NSLRKIKGA+RCAVN+VSYVDPPTPLKLADWF I GVFDFKTFTD PTSRPAMLGVSVVN
Sbjct: 361 NSLRKIKGAVRCAVNNVSYVDPPTPLKLADWFNIGGVFDFKTFTDFPTSRPAMLGVSVVN 420

Query: 421 ITLHDFVEIVFQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVY 480
           ITLHDFVEIVFQNNENTVQAWHFDGTSFYVVGYGSGVW Y MRKRYNLVDGISRHTVQVY
Sbjct: 421 ITLHDFVEIVFQNNENTVQAWHFDGTSFYVVGYGSGVWKYDMRKRYNLVDGISRHTVQVY 480

Query: 481 PRSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCGKA 540
           P+SWTAILVSLDNKGMWNLRSTIWWRNYLGQQLY RVWNDEKSLYTETDIPPNALKCGKA
Sbjct: 481 PKSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYFRVWNDEKSLYTETDIPPNALKCGKA 539

BLAST of CsGy2G014260 vs. NCBI nr
Match: TYJ99476.1 (L-ascorbate oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1036 bits (2680), Expect = 0.0
Identity = 500/533 (93.81%), Postives = 515/533 (96.62%), Query Frame = 0

Query: 9   NLSLGIIVLLVCCSLGWVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNGQFPAPKIECV 68
           NLSLGI+++ VCCSLGWVN EDPYRY+TFQVTYGTRAPLG  QKVILVNGQFPAPKIEC 
Sbjct: 3   NLSLGIMIIWVCCSLGWVNGEDPYRYYTFQVTYGTRAPLGVAQKVILVNGQFPAPKIECA 62

Query: 69  TNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGT 128
           TNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGT
Sbjct: 63  TNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGT 122

Query: 129 FTYFPSINFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDHKTLRKKLDSG 188
           FTYFPSINFQRASGGFGAFVIQPRSVITTPYP+P AE+DLLI DWY+TDHKTLRK+LDSG
Sbjct: 123 FTYFPSINFQRASGGFGAFVIQPRSVITTPYPLPHAEIDLLIGDWYDTDHKTLRKQLDSG 182

Query: 189 ILLPLPNRLLINGQPSLTLTLKKGLTYKLRISNVGIATSINFRIQGHLMTLVEVEGAHTV 248
           +LLPLP RLLINGQPS TL LKKGLTYKLRISNVGIATSINFRIQGHLMTLVEVEGAHTV
Sbjct: 183 VLLPLPKRLLINGQPSSTLILKKGLTYKLRISNVGIATSINFRIQGHLMTLVEVEGAHTV 242

Query: 249 QEVYESLDIHPGQSAAVLVSLNNLPKDYYFVVSSRFTKPTILTTVGILRYEGSSIPPSKP 308
           Q+VYESLDIHPGQSAAVLVSLN LPKDYYFVVSSRFTKP ILTTVGILRYEGSSIPPSKP
Sbjct: 243 QDVYESLDIHPGQSAAVLVSLNRLPKDYYFVVSSRFTKP-ILTTVGILRYEGSSIPPSKP 302

Query: 309 LPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENSLRKIKGAL 368
           LPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLIL+NSL KIKGA+
Sbjct: 303 LPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILKNSLTKIKGAV 362

Query: 369 RCAVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVVNITLHDFVEIV 428
           RCAVN+VSYVDPPTPLKLADWFKI GVFDFKTFTD+PT RPAMLGVSVVNITLHDFVEIV
Sbjct: 363 RCAVNNVSYVDPPTPLKLADWFKIGGVFDFKTFTDIPTPRPAMLGVSVVNITLHDFVEIV 422

Query: 429 FQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVYPRSWTAILVS 488
           FQN+ENTVQAWHFDGTSFYVVGYGSG WSYKMRKRYNLVDGISRHTVQVYPRSWTAILVS
Sbjct: 423 FQNDENTVQAWHFDGTSFYVVGYGSGDWSYKMRKRYNLVDGISRHTVQVYPRSWTAILVS 482

Query: 489 LDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCGKASKY 541
           LDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTE DIP NALKCGKASKY
Sbjct: 483 LDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTEADIPLNALKCGKASKY 534

BLAST of CsGy2G014260 vs. ExPASy TrEMBL
Match: A0A1S3C5Y6 (L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103497410 PE=3 SV=1)

HSP 1 Score: 1042 bits (2694), Expect = 0.0
Identity = 504/541 (93.16%), Postives = 521/541 (96.30%), Query Frame = 0

Query: 1   MREGNLVMNLSLGIIVLLVCCSLGWVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNGQF 60
           MREG ++ NLSLGI+++ VCCSLGWVN EDPYRY+TFQVTYGTRAPLG  QKVILVNGQF
Sbjct: 1   MREGKMI-NLSLGIMIIWVCCSLGWVNGEDPYRYYTFQVTYGTRAPLGVAQKVILVNGQF 60

Query: 61  PAPKIECVTNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKF 120
           PAPKIEC TNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKF
Sbjct: 61  PAPKIECATNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKF 120

Query: 121 QVKDQIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDHKT 180
           QVKDQIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYP+P AE+DLLI DWY+TDHKT
Sbjct: 121 QVKDQIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYPLPHAEIDLLIGDWYDTDHKT 180

Query: 181 LRKKLDSGILLPLPNRLLINGQPSLTLTLKKGLTYKLRISNVGIATSINFRIQGHLMTLV 240
           LRK+LDSG+LLPLP RLLINGQPS TL LKKGLTYKLRISNVGIATSINFRIQGHLMTLV
Sbjct: 181 LRKQLDSGVLLPLPKRLLINGQPSSTLILKKGLTYKLRISNVGIATSINFRIQGHLMTLV 240

Query: 241 EVEGAHTVQEVYESLDIHPGQSAAVLVSLNNLPKDYYFVVSSRFTKPTILTTVGILRYEG 300
           EVEGAHTVQ+VYESLDIHPGQSAAVLVSLN LPKDYYFVVSSRFTKP ILTTVGILRYEG
Sbjct: 241 EVEGAHTVQDVYESLDIHPGQSAAVLVSLNRLPKDYYFVVSSRFTKP-ILTTVGILRYEG 300

Query: 301 SSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENS 360
           SSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLIL+NS
Sbjct: 301 SSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILKNS 360

Query: 361 LRKIKGALRCAVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVVNIT 420
           L KIKGA+RCAVN+VSYVDPPTPLKLADWFKI GVFDFKTFTD+PT RPAMLGVSVVNIT
Sbjct: 361 LTKIKGAVRCAVNNVSYVDPPTPLKLADWFKIGGVFDFKTFTDIPTPRPAMLGVSVVNIT 420

Query: 421 LHDFVEIVFQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVYPR 480
           LHDFVEIVFQN+ENTVQAWHFDGTSFYVVGYGSG WSYKMRKRYNLVDGISRHTVQVYPR
Sbjct: 421 LHDFVEIVFQNDENTVQAWHFDGTSFYVVGYGSGDWSYKMRKRYNLVDGISRHTVQVYPR 480

Query: 481 SWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCGKASK 540
           SWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTE DIP NALKCGKASK
Sbjct: 481 SWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTEADIPLNALKCGKASK 539

BLAST of CsGy2G014260 vs. ExPASy TrEMBL
Match: A0A5D3BK26 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G00410 PE=3 SV=1)

HSP 1 Score: 1036 bits (2680), Expect = 0.0
Identity = 500/533 (93.81%), Postives = 515/533 (96.62%), Query Frame = 0

Query: 9   NLSLGIIVLLVCCSLGWVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNGQFPAPKIECV 68
           NLSLGI+++ VCCSLGWVN EDPYRY+TFQVTYGTRAPLG  QKVILVNGQFPAPKIEC 
Sbjct: 3   NLSLGIMIIWVCCSLGWVNGEDPYRYYTFQVTYGTRAPLGVAQKVILVNGQFPAPKIECA 62

Query: 69  TNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGT 128
           TNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGT
Sbjct: 63  TNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGT 122

Query: 129 FTYFPSINFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDHKTLRKKLDSG 188
           FTYFPSINFQRASGGFGAFVIQPRSVITTPYP+P AE+DLLI DWY+TDHKTLRK+LDSG
Sbjct: 123 FTYFPSINFQRASGGFGAFVIQPRSVITTPYPLPHAEIDLLIGDWYDTDHKTLRKQLDSG 182

Query: 189 ILLPLPNRLLINGQPSLTLTLKKGLTYKLRISNVGIATSINFRIQGHLMTLVEVEGAHTV 248
           +LLPLP RLLINGQPS TL LKKGLTYKLRISNVGIATSINFRIQGHLMTLVEVEGAHTV
Sbjct: 183 VLLPLPKRLLINGQPSSTLILKKGLTYKLRISNVGIATSINFRIQGHLMTLVEVEGAHTV 242

Query: 249 QEVYESLDIHPGQSAAVLVSLNNLPKDYYFVVSSRFTKPTILTTVGILRYEGSSIPPSKP 308
           Q+VYESLDIHPGQSAAVLVSLN LPKDYYFVVSSRFTKP ILTTVGILRYEGSSIPPSKP
Sbjct: 243 QDVYESLDIHPGQSAAVLVSLNRLPKDYYFVVSSRFTKP-ILTTVGILRYEGSSIPPSKP 302

Query: 309 LPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENSLRKIKGAL 368
           LPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLIL+NSL KIKGA+
Sbjct: 303 LPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILKNSLTKIKGAV 362

Query: 369 RCAVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVVNITLHDFVEIV 428
           RCAVN+VSYVDPPTPLKLADWFKI GVFDFKTFTD+PT RPAMLGVSVVNITLHDFVEIV
Sbjct: 363 RCAVNNVSYVDPPTPLKLADWFKIGGVFDFKTFTDIPTPRPAMLGVSVVNITLHDFVEIV 422

Query: 429 FQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVYPRSWTAILVS 488
           FQN+ENTVQAWHFDGTSFYVVGYGSG WSYKMRKRYNLVDGISRHTVQVYPRSWTAILVS
Sbjct: 423 FQNDENTVQAWHFDGTSFYVVGYGSGDWSYKMRKRYNLVDGISRHTVQVYPRSWTAILVS 482

Query: 489 LDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCGKASKY 541
           LDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTE DIP NALKCGKASKY
Sbjct: 483 LDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTEADIPLNALKCGKASKY 534

BLAST of CsGy2G014260 vs. ExPASy TrEMBL
Match: A0A6J1D5Y7 (L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111017933 PE=3 SV=1)

HSP 1 Score: 990 bits (2560), Expect = 0.0
Identity = 480/541 (88.72%), Postives = 501/541 (92.61%), Query Frame = 0

Query: 1   MREGNLVMNLSLGIIVLLVCCSLGWVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNGQF 60
           MREGNLV NL +GI  +L+CCS  WVNAEDPYRYFTFQV YGTRAPLG  QKVILVNGQF
Sbjct: 1   MREGNLV-NLIVGI--MLICCSAYWVNAEDPYRYFTFQVMYGTRAPLGVKQKVILVNGQF 60

Query: 61  PAPKIECVTNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKF 120
           PAPKIECVTNDNIIVNVINKLDEPFLFTWSG+KQRRTSWQDGVLGTNCPIPPNSNWTYKF
Sbjct: 61  PAPKIECVTNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTYKF 120

Query: 121 QVKDQIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDHKT 180
           QVKDQIGTFTYFPSINFQRA+GGFGAFVIQPRSVI TPYPIP AE DLLISDWY TDHK+
Sbjct: 121 QVKDQIGTFTYFPSINFQRAAGGFGAFVIQPRSVIATPYPIPDAEFDLLISDWYKTDHKS 180

Query: 181 LRKKLDSGILLPLPNRLLINGQPSLTLTLKKGLTYKLRISNVGIATSINFRIQGHLMTLV 240
           LRKKLDSGI LPLP+ LLING P+ TL LKKGLTYKLRISNVGIATSINFRIQGHLMTLV
Sbjct: 181 LRKKLDSGIQLPLPDALLINGLPTTTLILKKGLTYKLRISNVGIATSINFRIQGHLMTLV 240

Query: 241 EVEGAHTVQEVYESLDIHPGQSAAVLVSLNNLPKDYYFVVSSRFTKPTILTTVGILRYEG 300
           EVEGAHT+QE YESLDIHPGQSAAVL+SLNNLPKDY+FV SSRFTKP ILTT+GI+RYEG
Sbjct: 241 EVEGAHTMQEAYESLDIHPGQSAAVLISLNNLPKDYFFVASSRFTKP-ILTTIGIIRYEG 300

Query: 301 SSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENS 360
           SSI PSKPLPIGPTY LHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLIL NS
Sbjct: 301 SSILPSKPLPIGPTYHLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILANS 360

Query: 361 LRKIKGALRCAVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVVNIT 420
           L KIKG LRCAVN+VSYVDP TPLKLADWF I GVFD +TF D PT RPA LGVSVVNIT
Sbjct: 361 LTKIKGTLRCAVNNVSYVDPTTPLKLADWFNIPGVFDIRTFIDKPTPRPATLGVSVVNIT 420

Query: 421 LHDFVEIVFQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVYPR 480
           LHD+VEI+FQNNENTVQ WHFDGTSFYVVGYG G+W+Y MR+RYNLVDGISRHTVQVYPR
Sbjct: 421 LHDYVEIIFQNNENTVQVWHFDGTSFYVVGYGPGIWTYDMRRRYNLVDGISRHTVQVYPR 480

Query: 481 SWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCGKASK 540
           SWTAILVSLDNKGMWNLRSTIWWRNYLGQQLY +VWNDEKSLYTE DIP NALKCGKASK
Sbjct: 481 SWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYFKVWNDEKSLYTEADIPLNALKCGKASK 537

BLAST of CsGy2G014260 vs. ExPASy TrEMBL
Match: A0A6J1KZC3 (L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111499628 PE=3 SV=1)

HSP 1 Score: 987 bits (2552), Expect = 0.0
Identity = 481/544 (88.42%), Postives = 504/544 (92.65%), Query Frame = 0

Query: 1   MREGNLVMNLSLGIIVLLVCCSLG--WVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNG 60
           MREGNLV NL LGII  L CC+L   WVNAEDPYRYFTFQVTYGTRAPLG  QKVILVNG
Sbjct: 1   MREGNLV-NLILGII--LACCTLNTYWVNAEDPYRYFTFQVTYGTRAPLGVQQKVILVNG 60

Query: 61  QFPAPKIECVTNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTY 120
           QFPAP+IEC TNDNIIVNVINKLDEPFLFTWSG+KQRRTSWQDGVLGTNCPIPPNSNWTY
Sbjct: 61  QFPAPRIECETNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTY 120

Query: 121 KFQVKDQIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDH 180
           KFQVKDQIGTFTYFPSINFQRA+GGFGAFV+QPRSVITTPYPIP  E+DLLI DWYNTDH
Sbjct: 121 KFQVKDQIGTFTYFPSINFQRAAGGFGAFVVQPRSVITTPYPIPNGELDLLIGDWYNTDH 180

Query: 181 KTLRKKLDSGILLPLPNRLLINGQPSLTLTLKKGLTYKLRISNVGIATSINFRIQGHLMT 240
           KTLRK+L+SGI LPLPN LLING+ S TL LKKGLTYKLRISNVGIATSINFRIQGHLMT
Sbjct: 181 KTLRKRLESGIQLPLPNGLLINGKSSTTLVLKKGLTYKLRISNVGIATSINFRIQGHLMT 240

Query: 241 LVEVEGAHTVQEVYESLDIHPGQSAAVLVSLNNLP-KDYYFVVSSRFTKPTILTTVGILR 300
           LVEVEGAHT+QE YESLDIHPGQSAAVLVSL NL  K YYFV S+RFTKP IL  +GIL+
Sbjct: 241 LVEVEGAHTMQETYESLDIHPGQSAAVLVSLKNLASKSYYFVASTRFTKP-ILNAIGILK 300

Query: 301 YEGSSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLIL 360
           YEGS+ PPSKPLPIGPTY LHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLI+
Sbjct: 301 YEGSTTPPSKPLPIGPTYHLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLII 360

Query: 361 ENSLRKIKGALRCAVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVV 420
           ENSL KIKGALRCAVN+VSYVDPPTPLKLADWFKI GVFD  TFTD PTSRPAMLGVSVV
Sbjct: 361 ENSLTKIKGALRCAVNNVSYVDPPTPLKLADWFKIPGVFDINTFTDTPTSRPAMLGVSVV 420

Query: 421 NITLHDFVEIVFQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQV 480
           NITLHDFVEIVFQN E+TVQ+WHFDGTSFYVVGYGSGVW+Y MRKRYNLVDGISRHTVQV
Sbjct: 421 NITLHDFVEIVFQNKESTVQSWHFDGTSFYVVGYGSGVWTYNMRKRYNLVDGISRHTVQV 480

Query: 481 YPRSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCGK 540
           YP+SWTAILVSLDNKGMWNLRST+WWRNYLGQQLY +VWNDEKSLYTE DIP NA+KCGK
Sbjct: 481 YPKSWTAILVSLDNKGMWNLRSTVWWRNYLGQQLYFKVWNDEKSLYTEADIPLNAIKCGK 540

BLAST of CsGy2G014260 vs. ExPASy TrEMBL
Match: A0A6J1G5M8 (L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111451104 PE=3 SV=1)

HSP 1 Score: 985 bits (2547), Expect = 0.0
Identity = 479/544 (88.05%), Postives = 503/544 (92.46%), Query Frame = 0

Query: 1   MREGNLVMNLSLGIIVLLVCCSLG--WVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNG 60
           MREGNLV NL LGII  L CC+    WVNAEDPYRYFTFQVTYGTRAPLG  QK+ILVNG
Sbjct: 1   MREGNLV-NLILGII--LACCTFSTYWVNAEDPYRYFTFQVTYGTRAPLGVQQKIILVNG 60

Query: 61  QFPAPKIECVTNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTY 120
           QFPAP+IEC TNDNIIVNVINKLDEPFLFTWSG+KQRRTSWQDGVLGTNCPIPPNSNWTY
Sbjct: 61  QFPAPRIECETNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTY 120

Query: 121 KFQVKDQIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDH 180
           KFQVKDQIGTFTYFPSINFQRA+GGFGAFVIQPR+VITTPYPIP  E+DLLI DWYNTDH
Sbjct: 121 KFQVKDQIGTFTYFPSINFQRAAGGFGAFVIQPRTVITTPYPIPNGELDLLIGDWYNTDH 180

Query: 181 KTLRKKLDSGILLPLPNRLLINGQPSLTLTLKKGLTYKLRISNVGIATSINFRIQGHLMT 240
           KTLRKKL+SG+ LPLPN LLING+ S TL LKKGLTYKLRISNVGIATSINFRIQGHLMT
Sbjct: 181 KTLRKKLESGVQLPLPNGLLINGKSSTTLVLKKGLTYKLRISNVGIATSINFRIQGHLMT 240

Query: 241 LVEVEGAHTVQEVYESLDIHPGQSAAVLVSLNNLP-KDYYFVVSSRFTKPTILTTVGILR 300
           LVEVEGAHT+QE YESLDIHPGQSAAVLVSL NL  K YYFV S+RFTKP IL  +GIL+
Sbjct: 241 LVEVEGAHTMQETYESLDIHPGQSAAVLVSLKNLASKSYYFVASTRFTKP-ILNAIGILK 300

Query: 301 YEGSSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLIL 360
           YEGS+ PPSKPLPIGPTY LHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLI+
Sbjct: 301 YEGSTTPPSKPLPIGPTYHLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLII 360

Query: 361 ENSLRKIKGALRCAVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVV 420
           ENSL KIKGALRCAVN+VSYVDPPTPLKLADWFKI GVFD  TFTD PTSRPAMLGVSVV
Sbjct: 361 ENSLTKIKGALRCAVNNVSYVDPPTPLKLADWFKIPGVFDINTFTDTPTSRPAMLGVSVV 420

Query: 421 NITLHDFVEIVFQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQV 480
           NITLHDFVEIVFQN E+TVQ+WHFDGTSFYVVGYGSGVW+Y MRKRYNLVDGISRHTVQV
Sbjct: 421 NITLHDFVEIVFQNKESTVQSWHFDGTSFYVVGYGSGVWTYNMRKRYNLVDGISRHTVQV 480

Query: 481 YPRSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCGK 540
           YP+SWTAILVSLDNKGMWNLRST+WWRNYLGQQLY +VWNDEKSLYTE DIP NA+KCGK
Sbjct: 481 YPKSWTAILVSLDNKGMWNLRSTVWWRNYLGQQLYFKVWNDEKSLYTEADIPLNAIKCGK 540

BLAST of CsGy2G014260 vs. TAIR 10
Match: AT4G37160.1 (SKU5 similar 15 )

HSP 1 Score: 746.1 bits (1925), Expect = 2.0e-215
Identity = 353/517 (68.28%), Postives = 409/517 (79.11%), Query Frame = 0

Query: 26  VNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNGQFPAPKIECVTNDNIIVNVINKLDEPF 85
           VNAEDPY ++T+ VTYGTR+PLG  Q+VIL+NGQFP P IE VTN+NI+VN+INKLDEPF
Sbjct: 24  VNAEDPYMFYTWTVTYGTRSPLGVPQQVILINGQFPGPAIEAVTNNNIVVNLINKLDEPF 83

Query: 86  LFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGTFTYFPSINFQRASGGFG 145
           L TW+GVKQRRTSWQDGVLGTNCPI PNSNWTY+FQ+KDQIGT+TYF S +  RASG FG
Sbjct: 84  LITWNGVKQRRTSWQDGVLGTNCPIQPNSNWTYQFQLKDQIGTYTYFASTSLHRASGAFG 143

Query: 146 AFVIQPRSVITTPYPIPAAEVDLLISDWY-NTDHKTLRKKLDSGILLPLPNRLLING-QP 205
           A  I  RSVITTPYP P  +  LL+SDW+ N  HK LRK LD+G  LPLP+ LLING   
Sbjct: 144 ALNINQRSVITTPYPTPDGDFTLLVSDWFSNMTHKDLRKSLDAGSALPLPDALLINGVSK 203

Query: 206 SLTLTLKKGLTYKLRISNVGIATSINFRIQGHLMTLVEVEGAHTVQEVYESLDIHPGQSA 265
            L  T ++G TYK R+SNVGIATSINFRIQ H M+L+EVEGAHT+QE YESLD+H GQS 
Sbjct: 204 GLIFTGQQGKTYKFRVSNVGIATSINFRIQNHTMSLIEVEGAHTLQESYESLDVHVGQSM 263

Query: 266 AVLVSLNNLPKDYYFVVSSRFTKPTILTTVGILRYEGSSIPPSKPLPIGPTYQLHWSMKQ 325
            VLV+L    +DY+ V S+RFTKP +LTT   LRY+GS      PLPIGPTY +HWSMKQ
Sbjct: 264 TVLVTLKASVRDYFIVASTRFTKP-VLTTTASLRYQGSKNAAYGPLPIGPTYHIHWSMKQ 323

Query: 326 ARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENSLRKIKGALRCAVNSVSYVDPPTP 385
           ARTIR+NLTANAARPNPQGSFHYG I + R L+L N+   I G LR  VN +SY++P TP
Sbjct: 324 ARTIRMNLTANAARPNPQGSFHYGTIPINRTLVLANAATLIYGKLRYTVNRISYINPTTP 383

Query: 386 LKLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVVNITLHDFVEIVFQNNENTVQAWHFDG 445
           LKLADW+ I GVFDFKT    PT+ PA +G SV+++ LH+FVEIVFQN+E ++Q+WH DG
Sbjct: 384 LKLADWYNISGVFDFKTIISTPTTGPAHIGTSVIDVELHEFVEIVFQNDERSIQSWHMDG 443

Query: 446 TSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVYPRSWTAILVSLDNKGMWNLRSTIWW 505
           TS Y VGYGSG W+  MRKRYNLVD + RHT QVYP SWT ILVSLDNKGMWNLRS IW 
Sbjct: 444 TSAYAVGYGSGTWNVTMRKRYNLVDAVPRHTFQVYPLSWTTILVSLDNKGMWNLRSQIWS 503

Query: 506 RNYLGQQLYVRVWNDEKSLYTETDIPPNALKCGKASK 541
           R YLGQ+LYVRVWNDEKSLYTE + P N L CGKA +
Sbjct: 504 RRYLGQELYVRVWNDEKSLYTEAEPPLNVLYCGKAKR 539

BLAST of CsGy2G014260 vs. TAIR 10
Match: AT2G23630.1 (SKU5 similar 16 )

HSP 1 Score: 739.6 bits (1908), Expect = 1.8e-213
Identity = 354/530 (66.79%), Postives = 413/530 (77.92%), Query Frame = 0

Query: 10  LSLGIIVLLVCCSLGWVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNGQFPAPKIECVT 69
           L LG ++     S+  +NAEDPY +FT+ VTYGTR+PLG  Q+VIL+NGQFP P IE VT
Sbjct: 7   LLLGFLLAYCFSSVFVINAEDPYLFFTWTVTYGTRSPLGVPQQVILINGQFPGPPIEGVT 66

Query: 70  NDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGTF 129
           N+NI+VNVINKLDEPFL TW+G+KQR+ SWQDGVLGTNCPI P S+WTY FQ+KDQIGT+
Sbjct: 67  NNNIVVNVINKLDEPFLITWNGIKQRKMSWQDGVLGTNCPIQPKSSWTYHFQLKDQIGTY 126

Query: 130 TYFPSINFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDHKTLRKKLDSGI 189
            YF S +  RASG FGA  +  RSVI  PYP P A+  LL+SDWY   HK L+++LDS  
Sbjct: 127 AYFASTSMHRASGAFGALNVNQRSVIFVPYPKPDADFTLLVSDWYKMGHKELQRRLDSSR 186

Query: 190 LLPLPNRLLING-QPSLTLTLKKGLTYKLRISNVGIATSINFRIQGHLMTLVEVEGAHTV 249
            LP P+ LLING    L  T + G  Y+ RISNVGI+TSINFRIQGH+MTLVEVEG+HT+
Sbjct: 187 ALPPPDGLLINGASKGLVFTGQHGKIYRFRISNVGISTSINFRIQGHMMTLVEVEGSHTL 246

Query: 250 QEVYESLDIHPGQSAAVLVSLNNLPKDYYFVVSSRFTKPTILTTVGILRYEGSSIPPSKP 309
           QEVYESLDIH GQS  VLV+L    KDY+ V S+RFTKP ILTT GIL Y+GS I PS P
Sbjct: 247 QEVYESLDIHVGQSVTVLVTLKAPVKDYFIVASTRFTKP-ILTTTGILSYQGSKIRPSHP 306

Query: 310 LPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENSLRKIKGAL 369
           LPIGPTY +HWSMKQARTIRLNLTANAARPNPQGSFHYG I + R  +L N    I G L
Sbjct: 307 LPIGPTYHIHWSMKQARTIRLNLTANAARPNPQGSFHYGTIPINRTFVLANGRAMINGKL 366

Query: 370 RCAVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVVNITLHDFVEIV 429
           R  VN VSYV+P TPLKLADWF I GVF+FKT  ++PT  P++LG SV ++ LH++VE V
Sbjct: 367 RYTVNRVSYVNPATPLKLADWFNIPGVFNFKTIMNIPTPGPSILGTSVFDVALHEYVEFV 426

Query: 430 FQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVYPRSWTAILVS 489
           FQNNE ++Q+WH DGTS YVVGYGSG W+   R+ YNLVD +SRHT QVYP SWT+ILVS
Sbjct: 427 FQNNEGSIQSWHLDGTSAYVVGYGSGTWNMAKRRGYNLVDAVSRHTFQVYPMSWTSILVS 486

Query: 490 LDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCGKA 539
           LDNKGMWNLRS IW R YLGQ+LYVRVWN+EKSLYTE++ P N L CGKA
Sbjct: 487 LDNKGMWNLRSQIWSRRYLGQELYVRVWNNEKSLYTESEPPVNVLFCGKA 535

BLAST of CsGy2G014260 vs. TAIR 10
Match: AT5G66920.1 (SKU5 similar 17 )

HSP 1 Score: 647.1 bits (1668), Expect = 1.2e-185
Identity = 311/529 (58.79%), Postives = 384/529 (72.59%), Query Frame = 0

Query: 10  LSLGIIVLLVCCSLGWVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNGQFPAPKIECVT 69
           L LG + LL   SL  V  E PY+++T+ VTYG  +PLG  Q+VIL+NGQFP PK+E VT
Sbjct: 16  LLLGALTLL--SSLVIVKGESPYKFYTWTVTYGIISPLGVPQQVILINGQFPGPKLEVVT 75

Query: 70  NDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGTF 129
           NDNII+N+INKLD+PFL TW+G+KQR+ SWQDGVLGTNCPI PNSN+TYKFQ KDQIGTF
Sbjct: 76  NDNIILNLINKLDQPFLLTWNGIKQRKNSWQDGVLGTNCPIQPNSNFTYKFQTKDQIGTF 135

Query: 130 TYFPSINFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDHKTLRKKLDSGI 189
            YFPS  F +A+GGFGA  +  R  I  PYP+P A+  LL+ DW+ T+HKTL+++LDSG 
Sbjct: 136 NYFPSTAFHKAAGGFGAINVYARPGIPIPYPLPTADFTLLVGDWFKTNHKTLQQRLDSGG 195

Query: 190 LLPLPNRLLINGQPSLTLTLKKGLTYKLRISNVGIATSINFRIQGHLMTLVEVEGAHTVQ 249
           +LP P+ +LINGQ   T +  +G TY LRISNVG++++ NFRIQGH M +VEVEG+H +Q
Sbjct: 196 VLPFPDGMLINGQTQSTFSGDQGKTYMLRISNVGLSSTFNFRIQGHTMKVVEVEGSHVIQ 255

Query: 250 EVYESLDIHPGQSAAVLVSLNNLPKDYYFVVSSRFTKPTILTTVGILRYEGSSIPPSKPL 309
             Y+SLDIH GQS AVLV+LN  PKDYY V S+RF + + L+ +G+LRY  S +P S   
Sbjct: 256 TDYDSLDIHVGQSLAVLVTLNQSPKDYYIVASTRFIR-SKLSVMGLLRYSNSRVPASGDP 315

Query: 310 PIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENSLRKIKGALR 369
           P  P  +L WSM+QART R NLTANAARPNPQGSFHYG I+  +  +  NS   I G  R
Sbjct: 316 PALPPGELVWSMRQARTFRWNLTANAARPNPQGSFHYGMISPTKTFVFSNSAPLINGKQR 375

Query: 370 CAVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVVNITLHDFVEIVF 429
            AVN VSYV   TPLKLAD F I GVF       VP++ P  +  SVV  + HDF+EIVF
Sbjct: 376 YAVNGVSYVKSETPLKLADHFGISGVFSTNAIQSVPSNSPPTVATSVVQTSHHDFLEIVF 435

Query: 430 QNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVYPRSWTAILVSL 489
           QNNE ++Q+WH DG  F+VVG+GSG W+   R  +NLVD ++RHT QVYP SWT ILVSL
Sbjct: 436 QNNEKSMQSWHLDGYDFWVVGFGSGQWTPAKRSLHNLVDALTRHTTQVYPESWTTILVSL 495

Query: 490 DNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCGKA 539
           DN+GMWN+RS IW R Y GQQ Y++VWN  +SL  E + P N   CGKA
Sbjct: 496 DNQGMWNMRSAIWERQYSGQQFYLKVWNSVQSLANEYNPPDNLQLCGKA 541

BLAST of CsGy2G014260 vs. TAIR 10
Match: AT1G76160.1 (SKU5 similar 5 )

HSP 1 Score: 589.0 bits (1517), Expect = 4.0e-168
Identity = 285/515 (55.34%), Postives = 358/515 (69.51%), Query Frame = 0

Query: 26  VNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNGQFPAPKIECVTNDNIIVNVINKLDEPF 85
           V AEDPYR+F + +TYG   PLG  Q+ IL+NG FP P I  VTNDN+I+NV N LDEPF
Sbjct: 21  VTAEDPYRFFEWNITYGDIYPLGVRQQGILINGAFPGPDIHSVTNDNLIINVYNSLDEPF 80

Query: 86  LFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGTFTYFPSINFQRASGGFG 145
           L +W+G++QRR S+ DGV GT CPIPP  N+TY  Q+KDQIG+F YFPS+ F +A+GGFG
Sbjct: 81  LLSWNGIQQRRNSFVDGVYGTTCPIPPGKNYTYILQMKDQIGSFYYFPSLGFHKAAGGFG 140

Query: 146 AFVIQPRSVITTPYPIPAAEVDLLISDWYNTDHKTLRKKLDSGILLPLPNRLLINGQPS- 205
              I  R  I  P+P PA +  +LI DWY  +H  LR +LD+G  LPLP+ +LING+ S 
Sbjct: 141 GIRILSRPRIPVPFPDPAGDTTVLIGDWYKANHTDLRAQLDNGKKLPLPDGILINGRSSG 200

Query: 206 LTLTLKKGLTYKLRISNVGIATSINFRIQGHLMTLVEVEGAHTVQEVYESLDIHPGQSAA 265
            TL +++G TY+ RISNVG+  S+NFRIQ H M +VEVEG HT+Q  + SLD+H GQS +
Sbjct: 201 ATLNVEQGKTYRFRISNVGLQDSLNFRIQDHKMKVVEVEGTHTLQTTFSSLDVHVGQSYS 260

Query: 266 VLVSLNNLPKDYYFVVSSRFTKPTILTTVGILRYEGSSIPPSKPLPIGPTYQLHWSMKQA 325
           VLV+ +  P+DYY VVSSRFT   +LTT GI RY  S+   S P+P GPT Q+ WS+ QA
Sbjct: 261 VLVTADQTPRDYYVVVSSRFTS-NVLTTTGIFRYSNSAGGVSGPIPGGPTIQIDWSLNQA 320

Query: 326 RTIRLNLTANAARPNPQGSFHYGNITVVRRLILENSLRKIKGALRCAVNSVSYVDPPTPL 385
           R IR NL+A+  RPNPQGS+HYG I   R + L +S  ++ G  R AVNSVS+    TPL
Sbjct: 321 RAIRTNLSASGPRPNPQGSYHYGMINTTRTIRLASSAGQVDGKQRYAVNSVSFKPADTPL 380

Query: 386 KLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVVNITLHDFVEIVFQNNENTVQAWHFDGT 445
           K+AD+FKIDGV+   +    PT     L  SV+ +    FVEI+F+N+E+ VQ+WH DG 
Sbjct: 381 KIADYFKIDGVYRSGSIQYQPTGGGIYLDTSVMQVDYRTFVEIIFENSEDIVQSWHLDGY 440

Query: 446 SFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVYPRSWTAILVSLDNKGMWNLRSTIWWR 505
           SF+VVG   G WS   R  YNL D ++R TVQVYP SWTAIL++LDN GMWNLRS  W R
Sbjct: 441 SFWVVGMDGGQWSPDSRNEYNLRDAVARCTVQVYPSSWTAILIALDNVGMWNLRSEFWAR 500

Query: 506 NYLGQQLYVRVWNDEKSLYTETDIPPNALKCGKAS 540
            YLGQQLY+RV+    SL  E  IP NAL CG+AS
Sbjct: 501 QYLGQQLYLRVYTPSTSLRDEYPIPKNALLCGRAS 534

BLAST of CsGy2G014260 vs. TAIR 10
Match: AT1G41830.1 (SKU5-similar 6 )

HSP 1 Score: 587.8 bits (1514), Expect = 8.9e-168
Identity = 288/528 (54.55%), Postives = 365/528 (69.13%), Query Frame = 0

Query: 13  GIIVLLVCCS-LGWVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNGQFPAPKIECVTND 72
           G  +LL C S    V AE PYR+F + VTYG   PLG  Q+ IL+NGQFP P I  VTND
Sbjct: 8   GGTILLFCLSFFAAVTAESPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIHSVTND 67

Query: 73  NIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGTFTY 132
           N+I+NV N LDEPFL +W+GV+ RR S+ DG+ GT CPIPP SN+TY  QVKDQIG+F Y
Sbjct: 68  NLIINVHNSLDEPFLISWNGVQNRRNSYVDGMYGTTCPIPPRSNYTYILQVKDQIGSFYY 127

Query: 133 FPSINFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDHKTLRKKLDSGILL 192
           FPS+ F +A+GGFG   I  R  I  P+  PA +  +LI DWY  +H  L+ +LD G  L
Sbjct: 128 FPSLAFHKAAGGFGGIRILSRPGIPVPFADPAGDYTVLIGDWYKFNHTDLKSRLDRGRKL 187

Query: 193 PLPNRLLINGQPS-LTLTLKKGLTYKLRISNVGIATSINFRIQGHLMTLVEVEGAHTVQE 252
           P P+ +LING+ +  TL +++G TY+LRISNVG+  S+NFRIQ H M LVEVEG HT+Q 
Sbjct: 188 PSPDGILINGRSNGATLNVEQGKTYRLRISNVGLQDSLNFRIQNHRMKLVEVEGTHTLQT 247

Query: 253 VYESLDIHPGQSAAVLVSLNNLPKDYYFVVSSRFTKPTILTTVGILRYEGSSIPPSKPLP 312
           ++ SLD+H GQS +VL++ +  P+DYY VVSSRFT   I+TT G+LRY GSS P S P+P
Sbjct: 248 MFSSLDVHVGQSYSVLITADQSPRDYYVVVSSRFT-DKIITTTGVLRYSGSSTPASGPIP 307

Query: 313 IGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENSLRKIKGALRC 372
            GPT Q+ WS+ QAR IR NLTA+  RPNPQGS+HYG I ++R ++  +S  +I G  R 
Sbjct: 308 GGPTIQVDWSLNQARAIRTNLTASGPRPNPQGSYHYGLIPLIRTIVFGSSAGQINGKQRY 367

Query: 373 AVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVVNITLHDFVEIVFQ 432
            VNSVS+V   TPLKLAD+FKI GV+   + +D PT     L  SV+ +    F+EIVF+
Sbjct: 368 GVNSVSFVPADTPLKLADFFKISGVYKINSISDKPTYGGLYLDTSVLQVDYRTFIEIVFE 427

Query: 433 NNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVYPRSWTAILVSLD 492
           N E+ VQ++H +G SF+VVG   G W    R  YNL D +SR TVQVYP+SWTAI ++LD
Sbjct: 428 NQEDIVQSYHLNGYSFWVVGMDGGQWKTGSRNGYNLRDAVSRSTVQVYPKSWTAIYIALD 487

Query: 493 NKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCGKA 539
           N GMWNLRS  W R YLGQQLY+RV+    SL  E  IP N+  CG+A
Sbjct: 488 NVGMWNLRSEFWARQYLGQQLYLRVFTSSTSLRDEYPIPKNSRLCGRA 534

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q006242.9e-15550.37L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1[more]
P291628.4e-15549.53L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9SU401.9e-14648.36Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=... [more]
Q8VXX51.0e-14449.34Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=... [more]
Q9FHN61.2e-14047.57Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=... [more]
Match NameE-valueIdentityDescription
XP_004148037.10.0100.00L-ascorbate oxidase homolog [Cucumis sativus][more]
KAE8651923.10.0100.00hypothetical protein Csa_006507 [Cucumis sativus][more]
XP_008457817.10.093.16PREDICTED: L-ascorbate oxidase homolog [Cucumis melo][more]
XP_038902225.10.093.19L-ascorbate oxidase homolog [Benincasa hispida][more]
TYJ99476.10.093.81L-ascorbate oxidase-like protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A1S3C5Y60.093.16L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103497410 PE=3 SV=1[more]
A0A5D3BK260.093.81L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A6J1D5Y70.088.72L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111017933 PE=3 ... [more]
A0A6J1KZC30.088.42L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111499628 PE=3 SV=... [more]
A0A6J1G5M80.088.05L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111451104 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT4G37160.12.0e-21568.28SKU5 similar 15 [more]
AT2G23630.11.8e-21366.79SKU5 similar 16 [more]
AT5G66920.11.2e-18558.79SKU5 similar 17 [more]
AT1G76160.14.0e-16855.34SKU5 similar 5 [more]
AT1G41830.18.9e-16854.55SKU5-similar 6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008972CupredoxinGENE3D2.60.40.420coord: 164..337
e-value: 2.2E-31
score: 111.5
IPR008972CupredoxinGENE3D2.60.40.420coord: 31..154
e-value: 5.7E-35
score: 122.2
IPR008972CupredoxinGENE3D2.60.40.420coord: 347..540
e-value: 2.2E-37
score: 130.8
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 352..537
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 24..179
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 164..338
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 168..300
e-value: 1.2E-32
score: 113.3
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 383..520
e-value: 2.0E-27
score: 95.6
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 39..152
e-value: 3.7E-40
score: 136.6
NoneNo IPR availablePANTHERPTHR11709:SF245SKU5 SIMILAR 16coord: 8..538
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 8..538
IPR034273Ascorbate oxidase homologue, first cupredoxin domainCDDcd13846CuRO_1_AAO_like_1coord: 34..149
e-value: 3.61216E-71
score: 221.512

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy2G014260.2CsGy2G014260.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005507 copper ion binding
molecular_function GO:0008447 L-ascorbate oxidase activity
molecular_function GO:0016491 oxidoreductase activity