CsGy2G012280 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy2G012280
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionPectinesterase
LocationGy14Chr2: 12224419 .. 12227054 (+)
RNA-Seq ExpressionCsGy2G012280
SyntenyCsGy2G012280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCATGGGTCCTGGAGATATGACAACAGCAAGGCTAATAAGAGTGGATGGATCAGGAAATGGAGACTTCAAAAAAATTCAACAAGCAATTGATTCTGTTCCTTCTCAAAACAATGAACTCGTTTTCATTTGGGTTAAGCCTGGAACTTACAGGTAATTATTAATTGTTCCTTCATTTTCTTATTTTCATTTTGGTCACTCTTCTTCTTCTTCTTCTTCTTCTTTTCTCTCAATTTCTTTGATCGGGTGCTTGTAACACGATTATATGATTTGAACTCTTTAAGATTAGATCGAAGGAGAGAATTAGTTGAAATAAAGCATAGTTCAACCGGCATATTTAAACTTGTTATATGAGTTTAAGGTTAAGAAAATTGAGTTTTTAGTTTTCAAACTTGTGGTTGTTTTAACTTTTGAAGTTTTGAATTTGTTAACATTCTTCGTTCTTTATGTTTTAGGGTTCATTTTTTTATTATGGTCAACTTTTTTGTTATATTTATTGTTGTGTTGCGGTTGTGTCATGTTCGCATGGCCTCTTTCTGTTCATTTCTTTAAGTTTATATATACATGAAAAAAAAAAAAAGCAGAGAGAAGGAGAAGATAACAAAAGAAAAGAAATAGATGCGAGGATGAAGAAGGATCATAGAATTTTAAATAGTTCTATATTAGAAAAATATTGTGATTGAATAGGGTTTGATAGATTCTAAAATTTGGTTAAATTTTATATCGTTAGAAAGAATTTTATTTTTAAAAATTATATGGATAATTTTAATTAAAAACAATGACAACATATATACTTTTGTTTATGTGGTGTGTTGATAACAAATATTGAGTAGAGAGAAAATAGTGGTACCTGAGGACAAGCCCTACATAACAATAAGCGGCAGTAAGGCCTCCGATACAAAAATCACATGGAACCAAGGAAGAGATTTACTTGAATCTCCGGTTGTATCTATCTTTGCTTCTGATTTTGTCGGTCGTTTTCTGACAATCGAAAACACATTTGGCACAACTGGCATTGCGGTGGCATTAAGGGTTTCCGCCGATAGAGCAGCCTTTTACGGCTGTCGGATCATCTCCTTTCAAGACACCCTTTTGGACGACACTGGAAGACATTACTTCAATAATTGTTACATAGAAGGAGCCACCGATTTCATTTGCGGGAATGCCGCTTCTCTATACGAGGTTTTTTTTTCCATCTCTTGATATATTCATTACATACTAGCTAGTTAAGTTATATTTAAGGAGTATATATGGAAGGTTTCTTATATGTGGATTGTACTATAGAAGTGTCATTTGCATTCGACGTCGGATAGAGGTGGGGCGATGACGGCGCAGCATCGAAACACGGGGGAGGAAAACACAGGGTTCGTGTTCTTGGGAGGAAAAATAACAGGAAGTGGATCCATGTTTCTTGGACGGCCATGGGGGGATTTCTCTAAAGTTGTGTTTGGGTACACTTACATGTCCAATGTTGTTGAACCTGAGGGTTGGAATGATTGGGGAGATCCAACCAAGCAAAGGTAACATTATTATTTCTTTATAAGCTGTGATTTATAGCATATTTAAAATTACTTTTGTTATTTCTTTTTAAATCATTATAAAACATGTTTGTAATCCTTCAAGCTAATTTTAATTTTGTATAGATCAAAGTTGCATTTATGAAGTGTAAATTTCAAAAATTATTTTAAAAGAAAAGTGATTATGATGATTTTAAAATCACTACTCTTTCACTTAGTTCATTTGAATGGTTTTTGAAAATTTTAATTTAGTCTTAGTTACTTCGATATATAAGAATTTCCACAAAAACAAGGTGAAAAATGGTTTAAAAAATATATTTTTTATTTTCAATTTCAATGTTATCGTGTGAAAAGTTTTCATAATTTAGAAATATATATTACAAAGTCACAGTAAAGAAACTCTAATATACTATATGAAAAATTATTAAATTAAGAATCTCTACTATATACTAAATGAATTAAGTGAAAAATTATTAAATTTAAGAATTTAACTGATGTCTTAGGTGTTAACTTTTAGTTTATATAATTTTTAAAAGTTTTAATTTAGTCTTTGTTATTTGAATTTCCTTTCTGTAGTTTTACAAGTTAAAATAATTGCAAATATATATATACATATATATGAATAAAAAATTAAAAGAAAAACTACAAAAAAAATCTCTCTGAAACTTCGAAATAATTTCTTTCTCGCAAATTTATAACATTCCAACGCATAAATAAATTTTCTAGTTCAGATGATGTGAAAGCAAAACCTCGAATAGGGTTCTTTTATATAATGCTCGATTTGCTGGTCATTTTATTTTTTCTATATATTTAAATGCAGGGTTTGCTGTTTGTTTGTTGTTATATTTTACCAAAAACTCTACAAATATAATTAAGATTGGTAAAACTAAATGTAATTTGTAAATTAAGATTTTTGTGAAATCATGCACATGTATACAGAACGGTGTTGTATGGAGAATACAAATGTTATGGGCTCGGAGCAAATAGAGATAAAAGAGTGGTATGGTCACGTAGTTTGTCGACGGATGAGGCTTCAAAACTTTTCACCAAAGACATCATTGGTGGTCGAGCTTGGCTTCGACCTGCACCATCTCGTTTCAAAGGGGGCTTTCCCTAG

mRNA sequence

ATGGCCATGGGTCCTGGAGATATGACAACAGCAAGGCTAATAAGAGTGGATGGATCAGGAAATGGAGACTTCAAAAAAATTCAACAAGCAATTGATTCTGTTCCTTCTCAAAACAATGAACTCGTTTTCATTTGGGTTAAGCCTGGAACTTACAGAGAGAAAATAGTGGTACCTGAGGACAAGCCCTACATAACAATAAGCGGCAGTAAGGCCTCCGATACAAAAATCACATGGAACCAAGGAAGAGATTTACTTGAATCTCCGGTTGTATCTATCTTTGCTTCTGATTTTGTCGGTCGTTTTCTGACAATCGAAAACACATTTGGCACAACTGGCATTGCGGTGGCATTAAGGGTTTCCGCCGATAGAGCAGCCTTTTACGGCTGTCGGATCATCTCCTTTCAAGACACCCTTTTGGACGACACTGGAAGACATTACTTCAATAATTGTTACATAGAAGGAGCCACCGATTTCATTTGCGGGAATGCCGCTTCTCTATACGAGAAGTGTCATTTGCATTCGACGTCGGATAGAGGTGGGGCGATGACGGCGCAGCATCGAAACACGGGGGAGGAAAACACAGGGTTCGTGTTCTTGGGAGGAAAAATAACAGGAAGTGGATCCATGTTTCTTGGACGGCCATGGGGGGATTTCTCTAAAGTTGTGTTTGGGTACACTTACATGTCCAATGTTGTTGAACCTGAGGGTTGGAATGATTGGGGAGATCCAACCAAGCAAAGAACGGTGTTGTATGGAGAATACAAATGTTATGGGCTCGGAGCAAATAGAGATAAAAGAGTGGTATGGTCACGTAGTTTGTCGACGGATGAGGCTTCAAAACTTTTCACCAAAGACATCATTGGTGGTCGAGCTTGGCTTCGACCTGCACCATCTCGTTTCAAAGGGGGCTTTCCCTAG

Coding sequence (CDS)

ATGGCCATGGGTCCTGGAGATATGACAACAGCAAGGCTAATAAGAGTGGATGGATCAGGAAATGGAGACTTCAAAAAAATTCAACAAGCAATTGATTCTGTTCCTTCTCAAAACAATGAACTCGTTTTCATTTGGGTTAAGCCTGGAACTTACAGAGAGAAAATAGTGGTACCTGAGGACAAGCCCTACATAACAATAAGCGGCAGTAAGGCCTCCGATACAAAAATCACATGGAACCAAGGAAGAGATTTACTTGAATCTCCGGTTGTATCTATCTTTGCTTCTGATTTTGTCGGTCGTTTTCTGACAATCGAAAACACATTTGGCACAACTGGCATTGCGGTGGCATTAAGGGTTTCCGCCGATAGAGCAGCCTTTTACGGCTGTCGGATCATCTCCTTTCAAGACACCCTTTTGGACGACACTGGAAGACATTACTTCAATAATTGTTACATAGAAGGAGCCACCGATTTCATTTGCGGGAATGCCGCTTCTCTATACGAGAAGTGTCATTTGCATTCGACGTCGGATAGAGGTGGGGCGATGACGGCGCAGCATCGAAACACGGGGGAGGAAAACACAGGGTTCGTGTTCTTGGGAGGAAAAATAACAGGAAGTGGATCCATGTTTCTTGGACGGCCATGGGGGGATTTCTCTAAAGTTGTGTTTGGGTACACTTACATGTCCAATGTTGTTGAACCTGAGGGTTGGAATGATTGGGGAGATCCAACCAAGCAAAGAACGGTGTTGTATGGAGAATACAAATGTTATGGGCTCGGAGCAAATAGAGATAAAAGAGTGGTATGGTCACGTAGTTTGTCGACGGATGAGGCTTCAAAACTTTTCACCAAAGACATCATTGGTGGTCGAGCTTGGCTTCGACCTGCACCATCTCGTTTCAAAGGGGGCTTTCCCTAG

Protein sequence

MAMGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPYITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVSADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSRFKGGFP*
Homology
BLAST of CsGy2G012280 vs. ExPASy Swiss-Prot
Match: Q9SIJ9 (Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 6.5e-120
Identity = 197/297 (66.33%), Postives = 241/297 (81.14%), Query Frame = 0

Query: 7   DMTTARLIRVDGSGNGDFKKIQQAIDSVPS--QNNELVFIWVKPGTYREKIVVPEDKPYI 66
           D +TA LIRVD SG GDF KIQ+AI+S+P    N++L FIWVKPG YREK+V+P +KPYI
Sbjct: 44  DFSTAILIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYI 103

Query: 67  TISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVSADRA 126
           T+SG++AS+T + W+ G D+LESP ++IFASDFV RFLTI+N FGT G AVALRV+AD+A
Sbjct: 104 TLSGTQASNTFLIWSDGEDILESPTLTIFASDFVCRFLTIQNKFGTAGRAVALRVAADKA 163

Query: 127 AFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAMTA 186
           AFYGC I S+QDTLLDD G HYF NCYIEGATDFICG+A+SLYE+CHLHS S   G++TA
Sbjct: 164 AFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSLSPNNGSITA 223

Query: 187 QHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGDPT 246
           Q R +  E +GF FLG K+TGSGS FLGRPWG +S+VVF Y++ SNVV P+GWN WGD T
Sbjct: 224 QMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQWGDST 283

Query: 247 KQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSRFK 302
           K+ TV YGEYKCYG GA+R++RV WS+ LS +EA+   +KD IGG+ WLRPAPS FK
Sbjct: 284 KENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIGGKDWLRPAPSHFK 340

BLAST of CsGy2G012280 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.6e-73
Identity = 140/292 (47.95%), Postives = 186/292 (63.70%), Query Frame = 0

Query: 19  SGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPYITISGSKASDTKITW 78
           S  GDF KIQ AIDS+P  N   V I V  G Y+EK+ +P  K +ITI G  A  T + W
Sbjct: 91  SNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEW 150

Query: 79  N---QGRDLLESPV-------VSIFASDFVGRFLTIENTF-----GTTG-IAVALRVSAD 138
               Q  D   +P+        ++ +  FV + +T  NT      G  G  AVALRVSAD
Sbjct: 151 GDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSAD 210

Query: 139 RAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAM 198
            AAF+GCR++  QDTL D  GRHY+ +CYIEG+ DFI GNA SLYE CH+H+ +D+ GA+
Sbjct: 211 NAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAV 270

Query: 199 TAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGD 258
           TAQ R++  E+TGF F+  K+TG+G ++LGR WG FS+VVF YTYM N++ P GW +WGD
Sbjct: 271 TAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGD 330

Query: 259 PTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLR 295
           P+++ TV YG+YKC G GAN   RV W+R L+ +EA    +   I G  W++
Sbjct: 331 PSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 382

BLAST of CsGy2G012280 vs. ExPASy Swiss-Prot
Match: Q9FM79 (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 6.2e-70
Identity = 133/300 (44.33%), Postives = 188/300 (62.67%), Query Frame = 0

Query: 12  RLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPYITISG--S 71
           R+I VD +G GD   +Q A+D VP  N++ V I++ PG YREK++VP+ KPYI+  G  S
Sbjct: 80  RVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNES 139

Query: 72  KASDTKITWN--------QGRDL--LESPVVSIFASDFVGRFLTIENTF----GTTG-IA 131
            A DT I+W+         G++L    +  VSI +  F    +T ENT     G  G  A
Sbjct: 140 YAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTVVAEAGEQGRQA 199

Query: 132 VALRVSADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHS 191
           VALR+  D+A FY  R++  QDTL DD G HYF  CYI+G  DFI GNA SLY+ C +HS
Sbjct: 200 VALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHS 259

Query: 192 TSDRGGAMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEP 251
           T+ R GA+ A HR++  E+TGF F+   I+G+G ++LGR WG++S+ V+   ++++++ P
Sbjct: 260 TAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITP 319

Query: 252 EGWNDWGDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLR 295
            GW+DW  P +QR V++GEY C G GA R  RV WS++L+ DE      ++ I G  WLR
Sbjct: 320 VGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWLR 379

BLAST of CsGy2G012280 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.2e-65
Identity = 128/300 (42.67%), Postives = 179/300 (59.67%), Query Frame = 0

Query: 10  TARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPYITISGS 69
           T+ ++ VD  G G+F  +Q AID VP  ++    I V  G+YREK+ V E+K  + I G 
Sbjct: 87  TSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGR 146

Query: 70  KASDTKITWN----QGRDLLESPVVSIFASDFVGRFLTIENTF------GTTGIAVALRV 129
              +T I WN       +  +S    +FA++F    ++ +N             AVALR+
Sbjct: 147 GYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQAVALRI 206

Query: 130 SADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHS----- 189
             D+AAFYGC     QDTLLDD GRH+F  C+I+G+ DFI GN  SLY+ C ++S     
Sbjct: 207 EGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINSIAKGN 266

Query: 190 TSDRGGAMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEP 249
           TS   G++TAQ R + +E +GF F+  KI GSG + LGR WG ++ VVF  TYMS ++ P
Sbjct: 267 TSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITP 326

Query: 250 EGWNDWGDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLR 295
           EGWN+WGD TK++TV +GE+KCYG GA+  +RV++ + L+  EAS       I G  WLR
Sbjct: 327 EGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDGDEWLR 386

BLAST of CsGy2G012280 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 2.7e-65
Identity = 123/301 (40.86%), Postives = 176/301 (58.47%), Query Frame = 0

Query: 12  RLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPYITISGSKA 71
           ++I V  +G+  F+ +Q A+DS+P  NN+ + I + PG YREK+VVP  KPYIT  G+  
Sbjct: 59  KVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGR 118

Query: 72  SDTKITWN--------QGRDL--LESPVVSIFASDFVGRFLTIENTF-----GTTG-IAV 131
             T I W+         G+ L   ++  V+++A+ F  R ++  NT      G  G  AV
Sbjct: 119 DVTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAV 178

Query: 132 ALRVSADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHST 191
           A R+S D+A F GC     QDTL DD GRHYF  CYIEG+ DFI GN  S+Y+ C LHS 
Sbjct: 179 AFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSI 238

Query: 192 SDRGGAMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPE 251
           + R G++ A  R   EE TGF F+G ++TG+G +++GR  G +S++V+ YTY   +V   
Sbjct: 239 ASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHG 298

Query: 252 GWNDWGDPT-KQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLR 296
           GW+DW   + K +T  +G Y CYG GA   + V W+R+L  + A     K  + GR W+ 
Sbjct: 299 GWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWIA 358

BLAST of CsGy2G012280 vs. NCBI nr
Match: KGN61801.1 (hypothetical protein Csa_006548 [Cucumis sativus])

HSP 1 Score: 633 bits (1633), Expect = 2.57e-229
Identity = 304/305 (99.67%), Postives = 304/305 (99.67%), Query Frame = 0

Query: 1   MAMGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPED 60
           MAMGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPED
Sbjct: 1   MAMGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPED 60

Query: 61  KPYITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVS 120
           KPYITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVS
Sbjct: 61  KPYITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVS 120

Query: 121 ADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGG 180
           ADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGG
Sbjct: 121 ADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGG 180

Query: 181 AMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDW 240
           AMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDW
Sbjct: 181 AMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDW 240

Query: 241 GDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSRF 300
           GDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPS F
Sbjct: 241 GDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSHF 300

Query: 301 KGGFP 305
           KGGFP
Sbjct: 301 KGGFP 305

BLAST of CsGy2G012280 vs. NCBI nr
Match: XP_004148862.1 (putative pectinesterase 11 [Cucumis sativus])

HSP 1 Score: 633 bits (1633), Expect = 3.60e-229
Identity = 304/305 (99.67%), Postives = 304/305 (99.67%), Query Frame = 0

Query: 1   MAMGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPED 60
           MAMGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPED
Sbjct: 10  MAMGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPED 69

Query: 61  KPYITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVS 120
           KPYITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVS
Sbjct: 70  KPYITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVS 129

Query: 121 ADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGG 180
           ADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGG
Sbjct: 130 ADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGG 189

Query: 181 AMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDW 240
           AMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDW
Sbjct: 190 AMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDW 249

Query: 241 GDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSRF 300
           GDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPS F
Sbjct: 250 GDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSHF 309

Query: 301 KGGFP 305
           KGGFP
Sbjct: 310 KGGFP 314

BLAST of CsGy2G012280 vs. NCBI nr
Match: XP_008460943.2 (PREDICTED: LOW QUALITY PROTEIN: putative pectinesterase 11 [Cucumis melo])

HSP 1 Score: 590 bits (1522), Expect = 1.93e-212
Identity = 284/298 (95.30%), Postives = 289/298 (96.98%), Query Frame = 0

Query: 4   GPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPY 63
           GP D  TARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPY
Sbjct: 3   GPRDKRTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPY 62

Query: 64  ITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVSADR 123
           ITISGSKASDT ITWNQGRDLL+SPVVSIFASDFVGRFLTIENTFGT+GIAVALRVSADR
Sbjct: 63  ITISGSKASDTIITWNQGRDLLQSPVVSIFASDFVGRFLTIENTFGTSGIAVALRVSADR 122

Query: 124 AAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAMT 183
           AAFYGCRIISFQDTLLDD+GRHYFNNCYIEGATDFICGNAASLYE+CHLHSTSDRGGAMT
Sbjct: 123 AAFYGCRIISFQDTLLDDSGRHYFNNCYIEGATDFICGNAASLYERCHLHSTSDRGGAMT 182

Query: 184 AQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGDP 243
           AQHRNT EENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGDP
Sbjct: 183 AQHRNTAEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGDP 242

Query: 244 TKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSRFK 301
           TKQRTV YGEYKCYG GANR KRVVWSRSLSTDEA+KLFTKDIIGGRAWLRPAPS FK
Sbjct: 243 TKQRTVSYGEYKCYGPGANRAKRVVWSRSLSTDEATKLFTKDIIGGRAWLRPAPSHFK 300

BLAST of CsGy2G012280 vs. NCBI nr
Match: XP_038902622.1 (putative pectinesterase 11 [Benincasa hispida])

HSP 1 Score: 569 bits (1466), Expect = 6.37e-204
Identity = 272/301 (90.37%), Postives = 284/301 (94.35%), Query Frame = 0

Query: 3   MGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKP 62
           M P DMT+ARLIRVD SGNGDFKKIQ+AIDSVPS NNELVFIWVKPGTYREKIVVPE+KP
Sbjct: 1   MAPTDMTSARLIRVDQSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKP 60

Query: 63  YITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVSAD 122
           +ITISGSKASDT ITWNQG DLL+SPVVS+FASDF+GRFLTI+NTFGTTGIAVALRVSAD
Sbjct: 61  FITISGSKASDTIITWNQGTDLLQSPVVSVFASDFIGRFLTIQNTFGTTGIAVALRVSAD 120

Query: 123 RAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAM 182
           RAAFYGCRIISFQDTLLDD GRHYF NCYIEGATDFICGNAASLYE+CHLHSTSDRGGAM
Sbjct: 121 RAAFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHSTSDRGGAM 180

Query: 183 TAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGD 242
           TAQHRNT EENTGFVFLGGKITGSGS FLGRPWGD SKVVFGYTYMSNVVEP+GWNDWGD
Sbjct: 181 TAQHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEPQGWNDWGD 240

Query: 243 PTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSRFKG 302
            +KQRTVLYGEYKCYG GANR KRV WSRSLSTDEA+KLFTKDIIGGRAWLRPAPS FK 
Sbjct: 241 QSKQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLRPAPSHFKR 300

BLAST of CsGy2G012280 vs. NCBI nr
Match: XP_022947689.1 (putative pectinesterase 11 isoform X2 [Cucurbita moschata])

HSP 1 Score: 549 bits (1415), Expect = 4.71e-196
Identity = 261/299 (87.29%), Postives = 278/299 (92.98%), Query Frame = 0

Query: 3   MGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKP 62
           M P DM+TARLIRVD SG GDF+KIQ+AIDSVPS+NNELVFIW+KPGTYREKIVVPEDKP
Sbjct: 1   MAPADMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYREKIVVPEDKP 60

Query: 63  YITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVSAD 122
           +IT+SGSKASDT ITWNQGRDLL+SP +SIFA+DFVGRFLTI+NT+GTTGIAVALRVSAD
Sbjct: 61  FITLSGSKASDTIITWNQGRDLLQSPAISIFAADFVGRFLTIQNTYGTTGIAVALRVSAD 120

Query: 123 RAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAM 182
           RAAFYGCRIISFQDTLLDD GRHYF NCYIEGATDFICGNAASLYEKCHLHSTS+RGGAM
Sbjct: 121 RAAFYGCRIISFQDTLLDDVGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAM 180

Query: 183 TAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGD 242
           TAQHRNT  ENTGFVFLG KITGSGS  LGRPWGD S+VVFGYT+MSNVV PEGWNDWGD
Sbjct: 181 TAQHRNTVAENTGFVFLGCKITGSGSALLGRPWGDCSRVVFGYTHMSNVVVPEGWNDWGD 240

Query: 243 PTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSRFK 301
            TKQRTV YGEYKCYG GANR KRVVWSRSLSTDEA+KLFTKD+IGGR WLRPAPS FK
Sbjct: 241 HTKQRTVFYGEYKCYGPGANRAKRVVWSRSLSTDEAAKLFTKDMIGGRGWLRPAPSHFK 299

BLAST of CsGy2G012280 vs. ExPASy TrEMBL
Match: A0A0A0LP84 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G248110 PE=4 SV=1)

HSP 1 Score: 633 bits (1633), Expect = 1.25e-229
Identity = 304/305 (99.67%), Postives = 304/305 (99.67%), Query Frame = 0

Query: 1   MAMGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPED 60
           MAMGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPED
Sbjct: 1   MAMGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPED 60

Query: 61  KPYITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVS 120
           KPYITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVS
Sbjct: 61  KPYITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVS 120

Query: 121 ADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGG 180
           ADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGG
Sbjct: 121 ADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGG 180

Query: 181 AMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDW 240
           AMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDW
Sbjct: 181 AMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDW 240

Query: 241 GDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSRF 300
           GDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPS F
Sbjct: 241 GDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSHF 300

Query: 301 KGGFP 305
           KGGFP
Sbjct: 301 KGGFP 305

BLAST of CsGy2G012280 vs. ExPASy TrEMBL
Match: A0A1S3CDL2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499678 PE=4 SV=1)

HSP 1 Score: 590 bits (1522), Expect = 9.32e-213
Identity = 284/298 (95.30%), Postives = 289/298 (96.98%), Query Frame = 0

Query: 4   GPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPY 63
           GP D  TARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPY
Sbjct: 3   GPRDKRTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPY 62

Query: 64  ITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVSADR 123
           ITISGSKASDT ITWNQGRDLL+SPVVSIFASDFVGRFLTIENTFGT+GIAVALRVSADR
Sbjct: 63  ITISGSKASDTIITWNQGRDLLQSPVVSIFASDFVGRFLTIENTFGTSGIAVALRVSADR 122

Query: 124 AAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAMT 183
           AAFYGCRIISFQDTLLDD+GRHYFNNCYIEGATDFICGNAASLYE+CHLHSTSDRGGAMT
Sbjct: 123 AAFYGCRIISFQDTLLDDSGRHYFNNCYIEGATDFICGNAASLYERCHLHSTSDRGGAMT 182

Query: 184 AQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGDP 243
           AQHRNT EENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGDP
Sbjct: 183 AQHRNTAEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGDP 242

Query: 244 TKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSRFK 301
           TKQRTV YGEYKCYG GANR KRVVWSRSLSTDEA+KLFTKDIIGGRAWLRPAPS FK
Sbjct: 243 TKQRTVSYGEYKCYGPGANRAKRVVWSRSLSTDEATKLFTKDIIGGRAWLRPAPSHFK 300

BLAST of CsGy2G012280 vs. ExPASy TrEMBL
Match: A0A6J1G7K1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111451478 PE=4 SV=1)

HSP 1 Score: 549 bits (1415), Expect = 2.28e-196
Identity = 261/299 (87.29%), Postives = 278/299 (92.98%), Query Frame = 0

Query: 3   MGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKP 62
           M P DM+TARLIRVD SG GDF+KIQ+AIDSVPS+NNELVFIW+KPGTYREKIVVPEDKP
Sbjct: 1   MAPADMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYREKIVVPEDKP 60

Query: 63  YITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVSAD 122
           +IT+SGSKASDT ITWNQGRDLL+SP +SIFA+DFVGRFLTI+NT+GTTGIAVALRVSAD
Sbjct: 61  FITLSGSKASDTIITWNQGRDLLQSPAISIFAADFVGRFLTIQNTYGTTGIAVALRVSAD 120

Query: 123 RAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAM 182
           RAAFYGCRIISFQDTLLDD GRHYF NCYIEGATDFICGNAASLYEKCHLHSTS+RGGAM
Sbjct: 121 RAAFYGCRIISFQDTLLDDVGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAM 180

Query: 183 TAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGD 242
           TAQHRNT  ENTGFVFLG KITGSGS  LGRPWGD S+VVFGYT+MSNVV PEGWNDWGD
Sbjct: 181 TAQHRNTVAENTGFVFLGCKITGSGSALLGRPWGDCSRVVFGYTHMSNVVVPEGWNDWGD 240

Query: 243 PTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSRFK 301
            TKQRTV YGEYKCYG GANR KRVVWSRSLSTDEA+KLFTKD+IGGR WLRPAPS FK
Sbjct: 241 HTKQRTVFYGEYKCYGPGANRAKRVVWSRSLSTDEAAKLFTKDMIGGRGWLRPAPSHFK 299

BLAST of CsGy2G012280 vs. ExPASy TrEMBL
Match: A0A6J1G7A7 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111451478 PE=4 SV=1)

HSP 1 Score: 545 bits (1403), Expect = 1.59e-194
Identity = 261/300 (87.00%), Postives = 278/300 (92.67%), Query Frame = 0

Query: 3   MGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTY-REKIVVPEDK 62
           M P DM+TARLIRVD SG GDF+KIQ+AIDSVPS+NNELVFIW+KPGTY REKIVVPEDK
Sbjct: 1   MAPADMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYSREKIVVPEDK 60

Query: 63  PYITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVSA 122
           P+IT+SGSKASDT ITWNQGRDLL+SP +SIFA+DFVGRFLTI+NT+GTTGIAVALRVSA
Sbjct: 61  PFITLSGSKASDTIITWNQGRDLLQSPAISIFAADFVGRFLTIQNTYGTTGIAVALRVSA 120

Query: 123 DRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGA 182
           DRAAFYGCRIISFQDTLLDD GRHYF NCYIEGATDFICGNAASLYEKCHLHSTS+RGGA
Sbjct: 121 DRAAFYGCRIISFQDTLLDDVGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGA 180

Query: 183 MTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWG 242
           MTAQHRNT  ENTGFVFLG KITGSGS  LGRPWGD S+VVFGYT+MSNVV PEGWNDWG
Sbjct: 181 MTAQHRNTVAENTGFVFLGCKITGSGSALLGRPWGDCSRVVFGYTHMSNVVVPEGWNDWG 240

Query: 243 DPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSRFK 301
           D TKQRTV YGEYKCYG GANR KRVVWSRSLSTDEA+KLFTKD+IGGR WLRPAPS FK
Sbjct: 241 DHTKQRTVFYGEYKCYGPGANRAKRVVWSRSLSTDEAAKLFTKDMIGGRGWLRPAPSHFK 300

BLAST of CsGy2G012280 vs. ExPASy TrEMBL
Match: A0A6J1KXA2 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499612 PE=4 SV=1)

HSP 1 Score: 541 bits (1394), Expect = 3.48e-193
Identity = 256/299 (85.62%), Postives = 274/299 (91.64%), Query Frame = 0

Query: 3   MGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKP 62
           M P DM+TARLIRVD SG GDF+KIQ+AIDSVP +NNELVFIW+KPGTYREKIVVPEDKP
Sbjct: 1   MAPADMSTARLIRVDQSGKGDFRKIQEAIDSVPPRNNELVFIWIKPGTYREKIVVPEDKP 60

Query: 63  YITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVSAD 122
           +IT+SGSKASDT ITWNQGRDLL+SP +S+FASDFV RFLTI+NT+GTTGIAVALRVSAD
Sbjct: 61  FITLSGSKASDTIITWNQGRDLLQSPAISVFASDFVARFLTIQNTYGTTGIAVALRVSAD 120

Query: 123 RAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAM 182
           RAAFYGCRIISFQDTLLDD GRHYF NCYIEGATDFICGNAASLYEKCHLHS S+RGGAM
Sbjct: 121 RAAFYGCRIISFQDTLLDDVGRHYFKNCYIEGATDFICGNAASLYEKCHLHSMSERGGAM 180

Query: 183 TAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGD 242
           TAQHRNT  ENTGFVFLG KITGSGS  LGRPWGD S+VVFGYT+MSNVV PEGWNDWGD
Sbjct: 181 TAQHRNTTAENTGFVFLGCKITGSGSALLGRPWGDCSRVVFGYTHMSNVVVPEGWNDWGD 240

Query: 243 PTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSRFK 301
            TKQRTV YGEYKCYG G+NR KRV WSRSLSTDEA+KLFTKD+IGGR WLRPAPS FK
Sbjct: 241 HTKQRTVFYGEYKCYGPGSNRAKRVAWSRSLSTDEAAKLFTKDMIGGRGWLRPAPSHFK 299

BLAST of CsGy2G012280 vs. TAIR 10
Match: AT2G21610.1 (pectinesterase 11 )

HSP 1 Score: 431.8 bits (1109), Expect = 4.6e-121
Identity = 197/297 (66.33%), Postives = 241/297 (81.14%), Query Frame = 0

Query: 7   DMTTARLIRVDGSGNGDFKKIQQAIDSVPS--QNNELVFIWVKPGTYREKIVVPEDKPYI 66
           D +TA LIRVD SG GDF KIQ+AI+S+P    N++L FIWVKPG YREK+V+P +KPYI
Sbjct: 44  DFSTAILIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYI 103

Query: 67  TISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVSADRA 126
           T+SG++AS+T + W+ G D+LESP ++IFASDFV RFLTI+N FGT G AVALRV+AD+A
Sbjct: 104 TLSGTQASNTFLIWSDGEDILESPTLTIFASDFVCRFLTIQNKFGTAGRAVALRVAADKA 163

Query: 127 AFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAMTA 186
           AFYGC I S+QDTLLDD G HYF NCYIEGATDFICG+A+SLYE+CHLHS S   G++TA
Sbjct: 164 AFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSLSPNNGSITA 223

Query: 187 QHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGDPT 246
           Q R +  E +GF FLG K+TGSGS FLGRPWG +S+VVF Y++ SNVV P+GWN WGD T
Sbjct: 224 QMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQWGDST 283

Query: 247 KQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSRFK 302
           K+ TV YGEYKCYG GA+R++RV WS+ LS +EA+   +KD IGG+ WLRPAPS FK
Sbjct: 284 KENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIGGKDWLRPAPSHFK 340

BLAST of CsGy2G012280 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 277.7 bits (709), Expect = 1.1e-74
Identity = 140/292 (47.95%), Postives = 186/292 (63.70%), Query Frame = 0

Query: 19  SGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPYITISGSKASDTKITW 78
           S  GDF KIQ AIDS+P  N   V I V  G Y+EK+ +P  K +ITI G  A  T + W
Sbjct: 91  SNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEW 150

Query: 79  N---QGRDLLESPV-------VSIFASDFVGRFLTIENTF-----GTTG-IAVALRVSAD 138
               Q  D   +P+        ++ +  FV + +T  NT      G  G  AVALRVSAD
Sbjct: 151 GDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSAD 210

Query: 139 RAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAM 198
            AAF+GCR++  QDTL D  GRHY+ +CYIEG+ DFI GNA SLYE CH+H+ +D+ GA+
Sbjct: 211 NAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAV 270

Query: 199 TAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGD 258
           TAQ R++  E+TGF F+  K+TG+G ++LGR WG FS+VVF YTYM N++ P GW +WGD
Sbjct: 271 TAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGD 330

Query: 259 PTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLR 295
           P+++ TV YG+YKC G GAN   RV W+R L+ +EA    +   I G  W++
Sbjct: 331 PSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 382

BLAST of CsGy2G012280 vs. TAIR 10
Match: AT5G55590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 265.8 bits (678), Expect = 4.4e-71
Identity = 133/300 (44.33%), Postives = 188/300 (62.67%), Query Frame = 0

Query: 12  RLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPYITISG--S 71
           R+I VD +G GD   +Q A+D VP  N++ V I++ PG YREK++VP+ KPYI+  G  S
Sbjct: 80  RVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNES 139

Query: 72  KASDTKITWN--------QGRDL--LESPVVSIFASDFVGRFLTIENTF----GTTG-IA 131
            A DT I+W+         G++L    +  VSI +  F    +T ENT     G  G  A
Sbjct: 140 YAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTVVAEAGEQGRQA 199

Query: 132 VALRVSADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHS 191
           VALR+  D+A FY  R++  QDTL DD G HYF  CYI+G  DFI GNA SLY+ C +HS
Sbjct: 200 VALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHS 259

Query: 192 TSDRGGAMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEP 251
           T+ R GA+ A HR++  E+TGF F+   I+G+G ++LGR WG++S+ V+   ++++++ P
Sbjct: 260 TAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITP 319

Query: 252 EGWNDWGDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLR 295
            GW+DW  P +QR V++GEY C G GA R  RV WS++L+ DE      ++ I G  WLR
Sbjct: 320 VGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWLR 379

BLAST of CsGy2G012280 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 251.5 bits (641), Expect = 8.5e-67
Identity = 128/300 (42.67%), Postives = 179/300 (59.67%), Query Frame = 0

Query: 10  TARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPYITISGS 69
           T+ ++ VD  G G+F  +Q AID VP  ++    I V  G+YREK+ V E+K  + I G 
Sbjct: 87  TSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGR 146

Query: 70  KASDTKITWN----QGRDLLESPVVSIFASDFVGRFLTIENTF------GTTGIAVALRV 129
              +T I WN       +  +S    +FA++F    ++ +N             AVALR+
Sbjct: 147 GYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQAVALRI 206

Query: 130 SADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHS----- 189
             D+AAFYGC     QDTLLDD GRH+F  C+I+G+ DFI GN  SLY+ C ++S     
Sbjct: 207 EGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINSIAKGN 266

Query: 190 TSDRGGAMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEP 249
           TS   G++TAQ R + +E +GF F+  KI GSG + LGR WG ++ VVF  TYMS ++ P
Sbjct: 267 TSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITP 326

Query: 250 EGWNDWGDPTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLR 295
           EGWN+WGD TK++TV +GE+KCYG GA+  +RV++ + L+  EAS       I G  WLR
Sbjct: 327 EGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDGDEWLR 386

BLAST of CsGy2G012280 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 250.4 bits (638), Expect = 1.9e-66
Identity = 123/301 (40.86%), Postives = 176/301 (58.47%), Query Frame = 0

Query: 12  RLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPYITISGSKA 71
           ++I V  +G+  F+ +Q A+DS+P  NN+ + I + PG YREK+VVP  KPYIT  G+  
Sbjct: 59  KVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGR 118

Query: 72  SDTKITWN--------QGRDL--LESPVVSIFASDFVGRFLTIENTF-----GTTG-IAV 131
             T I W+         G+ L   ++  V+++A+ F  R ++  NT      G  G  AV
Sbjct: 119 DVTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAV 178

Query: 132 ALRVSADRAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHST 191
           A R+S D+A F GC     QDTL DD GRHYF  CYIEG+ DFI GN  S+Y+ C LHS 
Sbjct: 179 AFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSI 238

Query: 192 SDRGGAMTAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPE 251
           + R G++ A  R   EE TGF F+G ++TG+G +++GR  G +S++V+ YTY   +V   
Sbjct: 239 ASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHG 298

Query: 252 GWNDWGDPT-KQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLR 296
           GW+DW   + K +T  +G Y CYG GA   + V W+R+L  + A     K  + GR W+ 
Sbjct: 299 GWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWIA 358

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SIJ96.5e-12066.33Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1[more]
Q8VYZ31.6e-7347.95Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1[more]
Q9FM796.2e-7044.33Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1[more]
Q9ZQA31.2e-6542.67Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1[more]
Q8LPF32.7e-6540.86Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KGN61801.12.57e-22999.67hypothetical protein Csa_006548 [Cucumis sativus][more]
XP_004148862.13.60e-22999.67putative pectinesterase 11 [Cucumis sativus][more]
XP_008460943.21.93e-21295.30PREDICTED: LOW QUALITY PROTEIN: putative pectinesterase 11 [Cucumis melo][more]
XP_038902622.16.37e-20490.37putative pectinesterase 11 [Benincasa hispida][more]
XP_022947689.14.71e-19687.29putative pectinesterase 11 isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0LP841.25e-22999.67Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G248110 PE=4 SV=1[more]
A0A1S3CDL29.32e-21395.30Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499678 PE=4 SV=1[more]
A0A6J1G7K12.28e-19687.29Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111451478 PE=4 SV=1[more]
A0A6J1G7A71.59e-19487.00Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111451478 PE=4 SV=1[more]
A0A6J1KXA23.48e-19385.62Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499612 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G21610.14.6e-12166.33pectinesterase 11 [more]
AT5G19730.11.1e-7447.95Pectin lyase-like superfamily protein [more]
AT5G55590.14.4e-7144.33Pectin lyase-like superfamily protein [more]
AT2G36710.18.5e-6742.67Pectin lyase-like superfamily protein [more]
AT5G47500.11.9e-6640.86Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 17..284
e-value: 9.0E-64
score: 215.0
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 8..303
e-value: 1.1E-101
score: 342.4
NoneNo IPR availablePANTHERPTHR31321:SF77PECTINESTERASE 11-RELATEDcoord: 8..295
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 8..295
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 9..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy2G012280.1CsGy2G012280.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity