CsGy2G005908 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy2G005908
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description(R)-mandelonitrile lyase 1-like
LocationGy14Chr2: 4198892 .. 4201229 (-)
RNA-Seq ExpressionCsGy2G005908
SyntenyCsGy2G005908
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAACATTCTAGGGTTACCATCCTCATACTCAGCCTTATGATCTCTATCTTTCAATTACAACTTCACTCCTCACATGCTATCCCCAATCAAGGTACAAATTATTACAGAATAAGTTTTCTTCACAAATCGGATGACAGTTTTACAACGTTCGTTACATGTCGCCTTTTTTTTGTTAAAAAATTTCCTTCATTGATACTTATTTTGGAATGAGCTTAAATTTTCAGATGTTAGCTACATGAAGTTTGTTCATAATGCCACTGATTTACCACCAAAAGAAGAATATGACTACATAATTATAGGAGGTGGAACTGCAGGGTGTCCATTAGCTACAACATTATCATCAAAATTCTCAGTCCTCCTTTTAGAAAGAGGCAGTGACCCCAACAAATATCCTTCTGTGTTGAATGAACAAGGTCTATTGAACGTTTTTGCTGCTGGAGATGATGGAAGAAATCCCTTTCAACGTTTCGTCTCGGAGGATGGCGTAGAGAACATACGAGGACGAGTCCTTGGCGGAGGAAGCATGGTCAATGCCGGGTTCTATTCGAGGGGACATCGAGAGTTTTTCGCAAGTGCAGGTGTTGATTGGGACATGGAATTGGTTGAGAAGGCTTATGAATGGGTTGAAGAAACTGTTGTATCTCAACCAATTTTGAATGCTTGGCAATCTGCTTTTAGAAGTTCTTTATTGGAAGGTGGTGTTGTCCCTGATAATGGGTTTGATTTAAGGCATCTTGTGGGAACTAAAACTGGAGGTTCAATCTTTGATAATAAAGGAAATAGACATGGAGCTGTGGAGCTTCTTAATAAGGCTAATCCCACAAATATTAAAGTTGCTATTGAAGCCACAGTTCAAAGAATCCTCTTCTCTGGTTAGTGCTTGCAAATATTCCATTTAAATTATATTTGTTTTAAATGACTGGAGTTTTAATTTATACTTTAACGTAATCTCATGAGGAAACCTGAGCCAATGACTTTATGTATTGTTCCATTGAGATAATTTTACCAACTCCGAGAAATTGTTTTCTAGGATTTAACAAAGTACATTTCTTGTTGAAGCAACACTCTTTCACAAGATTTTTTTGAAAATAAATTGAACTTGAAAAAAGCTTATTTCAAGTTCAATTTAACTCGTGAAAAGTTATTCTAAAATGCATTAATTTTGAAGAAAGTCATATTTCGAGTTCATATCATTTATTCCAACCGATAATTTCCACAGTTTTTTATCATGTATTATTTTGACTCGTAGGTTTATCAGCAAATGGGGTGTTGTATTCAGATTCAAAAGGAAAGCTACACAGAGCAATCATTCGCAAAAAGGGAGAAATCATTGTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTTCTAAGTGGGATTGGCCCAAAATCTCATCTTTCATCCTTAAAACTACCTGTCGTTCTTCACCAACCATATGTCGGCCAATCCATGTCCGACAATCCCCGTTTTGGCACCAACATCATCATTCCATTTCCAGTTCTTCCTTCTTCTGTAAAAGTTGTTGGAATTTTACAAGACAATATCTATATACAATCTATTGCCAGCCCTTTCCCAATTTTAATTCCCCAAATTTTCAGTCTTCTTCCTCCTCAAGCCACTTCCATCATCCCAACCTTAGCCATGTTTGTTGGAAAATTCTCCGAAGTTCATTCTGAAGGCTCACTTCGATTAAATTCCTCTACTAATGTGAAAAAGAGTCCCATTGTTGGATTCAACTATTATTCACATCCTGATGATCTTGGTCGATGTGTTAAAGGTGTAAGAAAAATGGGAGATTTGCTTAAAACTCGAACTATGGAAAAGATTAAGACAAAAAATTTGGAGGGTAATAAAGGGTTTGAGTTCTTGGGTGTTCCACTACCGGAAAATTTGTGGAATGATAGCTCTGTTGAAGAATATTGTAAAAAGACAGTGACTACTTATTGGCATTACCATGGAGGATGTTTGGTGGGAAAAGTGGTTGACGGTAATTATAAAGTGATTGGAATTAAAAATCTGCGTGTTGTCGATGGCTCCACTTTCTCTGAGTCGCCGGGAACTAATCCCATGGCCACTCTCATGATGCTTGGCCGGTGAGTAATTCCTTTTTTTTTCTCTCTATGTTTGCAACTATAGGATTATCAAAACTTTGAAGTTTTCTTTCAAAAAAAAAAAAAAGTAACACCATATAATATGTTCATATAAAGAAATAGGATGTAACTTGTGATTTAATTTCAATATTCTCTTTTATTTGAATGAACTAATCATTTCAAAATAATTTTGCAGATATGTTGGCCTTAAGTTACTCCATCAAAGATCGAATCTCAAATAA

mRNA sequence

ATGGAACATTCTAGGGTTACCATCCTCATACTCAGCCTTATGATCTCTATCTTTCAATTACAACTTCACTCCTCACATGCTATCCCCAATCAAGATGTTAGCTACATGAAGTTTGTTCATAATGCCACTGATTTACCACCAAAAGAAGAATATGACTACATAATTATAGGAGGTGGAACTGCAGGGTGTCCATTAGCTACAACATTATCATCAAAATTCTCAGTCCTCCTTTTAGAAAGAGGCAGTGACCCCAACAAATATCCTTCTGTGTTGAATGAACAAGGTCTATTGAACGTTTTTGCTGCTGGAGATGATGGAAGAAATCCCTTTCAACGTTTCGTCTCGGAGGATGGCGTAGAGAACATACGAGGACGAGTCCTTGGCGGAGGAAGCATGGTCAATGCCGGGTTCTATTCGAGGGGACATCGAGAGTTTTTCGCAAGTGCAGGTGTTGATTGGGACATGGAATTGGTTGAGAAGGCTTATGAATGGGTTGAAGAAACTGTTGTATCTCAACCAATTTTGAATGCTTGGCAATCTGCTTTTAGAAGTTCTTTATTGGAAGGTGGTGTTGTCCCTGATAATGGGTTTGATTTAAGGCATCTTGTGGGAACTAAAACTGGAGGTTCAATCTTTGATAATAAAGGAAATAGACATGGAGCTGTGGAGCTTCTTAATAAGGCTAATCCCACAAATATTAAAGTTGCTATTGAAGCCACAGTTCAAAGAATCCTCTTCTCTGGTTTATCAGCAAATGGGGTGTTGTATTCAGATTCAAAAGGAAAGCTACACAGAGCAATCATTCGCAAAAAGGGAGAAATCATTGTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTTCTAAGTGGGATTGGCCCAAAATCTCATCTTTCATCCTTAAAACTACCTGTCGTTCTTCACCAACCATATGTCGGCCAATCCATGTCCGACAATCCCCGTTTTGGCACCAACATCATCATTCCATTTCCAGTTCTTCCTTCTTCTGTAAAAGTTGTTGGAATTTTACAAGACAATATCTATATACAATCTATTGCCAGCCCTTTCCCAATTTTAATTCCCCAAATTTTCAGTCTTCTTCCTCCTCAAGCCACTTCCATCATCCCAACCTTAGCCATGTTTGTTGGAAAATTCTCCGAAGTTCATTCTGAAGGCTCACTTCGATTAAATTCCTCTACTAATGTGAAAAAGAGTCCCATTGTTGGATTCAACTATTATTCACATCCTGATGATCTTGGTCGATGTGTTAAAGGTGTAAGAAAAATGGGAGATTTGCTTAAAACTCGAACTATGGAAAAGATTAAGACAAAAAATTTGGAGGGTAATAAAGGGTTTGAGTTCTTGGGTGTTCCACTACCGGAAAATTTGTGGAATGATAGCTCTGTTGAAGAATATTGTAAAAAGACAGTGACTACTTATTGGCATTACCATGGAGGATGTTTGGTGGGAAAAGTGGTTGACGGTAATTATAAAGTGATTGGAATTAAAAATCTGCGTGTTGTCGATGGCTCCACTTTCTCTGAGTCGCCGGGAACTAATCCCATGGCCACTCTCATGATGCTTGGCCGATATGTTGGCCTTAAGTTACTCCATCAAAGATCGAATCTCAAATAA

Coding sequence (CDS)

ATGGAACATTCTAGGGTTACCATCCTCATACTCAGCCTTATGATCTCTATCTTTCAATTACAACTTCACTCCTCACATGCTATCCCCAATCAAGATGTTAGCTACATGAAGTTTGTTCATAATGCCACTGATTTACCACCAAAAGAAGAATATGACTACATAATTATAGGAGGTGGAACTGCAGGGTGTCCATTAGCTACAACATTATCATCAAAATTCTCAGTCCTCCTTTTAGAAAGAGGCAGTGACCCCAACAAATATCCTTCTGTGTTGAATGAACAAGGTCTATTGAACGTTTTTGCTGCTGGAGATGATGGAAGAAATCCCTTTCAACGTTTCGTCTCGGAGGATGGCGTAGAGAACATACGAGGACGAGTCCTTGGCGGAGGAAGCATGGTCAATGCCGGGTTCTATTCGAGGGGACATCGAGAGTTTTTCGCAAGTGCAGGTGTTGATTGGGACATGGAATTGGTTGAGAAGGCTTATGAATGGGTTGAAGAAACTGTTGTATCTCAACCAATTTTGAATGCTTGGCAATCTGCTTTTAGAAGTTCTTTATTGGAAGGTGGTGTTGTCCCTGATAATGGGTTTGATTTAAGGCATCTTGTGGGAACTAAAACTGGAGGTTCAATCTTTGATAATAAAGGAAATAGACATGGAGCTGTGGAGCTTCTTAATAAGGCTAATCCCACAAATATTAAAGTTGCTATTGAAGCCACAGTTCAAAGAATCCTCTTCTCTGGTTTATCAGCAAATGGGGTGTTGTATTCAGATTCAAAAGGAAAGCTACACAGAGCAATCATTCGCAAAAAGGGAGAAATCATTGTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTTCTAAGTGGGATTGGCCCAAAATCTCATCTTTCATCCTTAAAACTACCTGTCGTTCTTCACCAACCATATGTCGGCCAATCCATGTCCGACAATCCCCGTTTTGGCACCAACATCATCATTCCATTTCCAGTTCTTCCTTCTTCTGTAAAAGTTGTTGGAATTTTACAAGACAATATCTATATACAATCTATTGCCAGCCCTTTCCCAATTTTAATTCCCCAAATTTTCAGTCTTCTTCCTCCTCAAGCCACTTCCATCATCCCAACCTTAGCCATGTTTGTTGGAAAATTCTCCGAAGTTCATTCTGAAGGCTCACTTCGATTAAATTCCTCTACTAATGTGAAAAAGAGTCCCATTGTTGGATTCAACTATTATTCACATCCTGATGATCTTGGTCGATGTGTTAAAGGTGTAAGAAAAATGGGAGATTTGCTTAAAACTCGAACTATGGAAAAGATTAAGACAAAAAATTTGGAGGGTAATAAAGGGTTTGAGTTCTTGGGTGTTCCACTACCGGAAAATTTGTGGAATGATAGCTCTGTTGAAGAATATTGTAAAAAGACAGTGACTACTTATTGGCATTACCATGGAGGATGTTTGGTGGGAAAAGTGGTTGACGGTAATTATAAAGTGATTGGAATTAAAAATCTGCGTGTTGTCGATGGCTCCACTTTCTCTGAGTCGCCGGGAACTAATCCCATGGCCACTCTCATGATGCTTGGCCGATATGTTGGCCTTAAGTTACTCCATCAAAGATCGAATCTCAAATAA

Protein sequence

MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQRSNLK*
Homology
BLAST of CsGy2G005908 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 8.7e-160
Identity = 282/548 (51.46%), Postives = 381/548 (69.53%), Query Frame = 0

Query: 1   MEHSRVTILILSLMISIFQLQ---LHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIG 60
           ME S ++ ++L L + +  LQ   +HS     N D SY++F ++ATDL  +  YDY+I+G
Sbjct: 1   MEKSTMSAILLVLHLFVLLLQYSEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVG 60

Query: 61  GGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGRNPFQRFVSED 120
           GGT+GCPLA TLS K+ VL+LERGS P  YP+VL   G +      DDG+ P +RFVSED
Sbjct: 61  GGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSED 120

Query: 121 GVENIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNA 180
           G++N+RGRVLGG SM+NAG Y+R +   ++++GVDWDM+LV K YEWVE+T+V +P    
Sbjct: 121 GIDNVRGRVLGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQP 180

Query: 181 WQSAFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAI 240
           WQS   ++ LE GV P++GF L H  GT+  GS FDNKG RH A ELLNK N  N++V +
Sbjct: 181 WQSVTGTAFLEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGV 240

Query: 241 EATVQRILFS---GLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGP 300
            A+V++I+FS   GL+A GV+Y DS G  HRA +R KGE+IVSAG IG+PQLLLLSG+GP
Sbjct: 241 HASVEKIIFSNAPGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGP 300

Query: 301 KSHLSSLKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIAS 360
           +S+LSSL +PVVL  PYVGQ + DNPR   NI+ P P+ P+ V V+GI  D  + Q   S
Sbjct: 301 ESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGISND--FYQCSFS 360

Query: 361 PFPILIPQIFSLLPPQATSI-IPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYY 420
             P   P  FS  P  +  +   T A F  K +   S GSL L SS+NV+ SP V FNYY
Sbjct: 361 SLPFTTPP-FSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYY 420

Query: 421 SHPDDLGRCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYC 480
           S+P DL  CV G++K+G+LL T  ++  K ++L G +GF  LG+PLP++  +D++ E +C
Sbjct: 421 SNPTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFC 480

Query: 481 KKTVTTYWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVG 540
           +++V +YWHYHGGCLVGKV+DG+++V GI  LRVVDGSTF  +P ++P    +MLGRYVG
Sbjct: 481 RESVASYWHYHGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLGRYVG 540

Query: 541 LKLLHQRS 542
           +K+L +RS
Sbjct: 541 IKILQERS 545

BLAST of CsGy2G005908 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 2.4e-157
Identity = 279/549 (50.82%), Postives = 382/549 (69.58%), Query Frame = 0

Query: 1   MEHSRVTILILSLMISIFQLQ---LHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIG 60
           ME S ++ ++L L I +  LQ   +HS     + D SY+ F ++ATDL  +  YDY+I+G
Sbjct: 1   MEKSTMSAILLVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVG 60

Query: 61  GGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGRNPFQRFVSED 120
           GGT+GCPLA TLS K+ VL+LERGS P  YP+VL   G +      DDG+ P +RFVSED
Sbjct: 61  GGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSED 120

Query: 121 GVENIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNA 180
           G++N+RGRVLGG S++NAG Y+R +   ++++GVDWDM+LV + YEWVE+T+V +P   +
Sbjct: 121 GIDNVRGRVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPNSQS 180

Query: 181 WQSAFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAI 240
           WQS  +++ LE GV P++GF L H  GT+  GS FDNKG RH A ELLNK N  N++V +
Sbjct: 181 WQSVTKTAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGV 240

Query: 241 EATVQRILFS---GLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGP 300
            A+V++I+FS   GL+A GV+Y DS G  H+A +R KGE+IVSAG IG+PQLLLLSG+GP
Sbjct: 241 HASVEKIIFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGP 300

Query: 301 KSHLSSLKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIAS 360
           +S+LSSL +PVVL  PYVGQ + DNPR   NI+ P P+ P+ V V+GI  D  + Q   S
Sbjct: 301 ESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGISND--FYQCSFS 360

Query: 361 PFPILIPQIFSLLPPQATSIIP--TLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNY 420
             P   P  F    P A+  +P  T A F  K +   S GSL L SS+NV+ SP V FNY
Sbjct: 361 SLPFTTPP-FGFF-PSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNY 420

Query: 421 YSHPDDLGRCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEY 480
           YS+  DL  CV G++K+G+LL T  ++  K ++L G +GF  LG+PLP++  +D++ E +
Sbjct: 421 YSNLTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETF 480

Query: 481 CKKTVTTYWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYV 540
           C+++V +YWHYHGGCLVGKV+DG+++V GI  LRVVDGSTF  +P ++P    +MLGRYV
Sbjct: 481 CRESVASYWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLGRYV 540

Query: 541 GLKLLHQRS 542
           G+K+L +RS
Sbjct: 541 GIKILQERS 545

BLAST of CsGy2G005908 vs. ExPASy Swiss-Prot
Match: O82784 ((R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 2.0e-151
Identity = 272/550 (49.45%), Postives = 371/550 (67.45%), Query Frame = 0

Query: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPN----QDVSYMKFVHNATDLPPKEEYDYIII 60
           ME S ++ ++L L + +  LQ    H++ N     D  Y+KFV+NA DL  +  YDYII+
Sbjct: 1   MEKSTMSAVVLVLNLLVLHLQYSEVHSLANTSSEHDFGYLKFVYNAVDLELEGSYDYIIV 60

Query: 61  GGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGRNPFQRFVSE 120
           GGGT+GCPLA TLS+ +SVL+LERG+   +YP+ L   G        DDG+ P +RFVSE
Sbjct: 61  GGGTSGCPLAATLSANYSVLVLERGTIATEYPNTLTVDGFAYNLQQQDDGKTPVERFVSE 120

Query: 121 DGVENIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILN 180
           DG++N+R R+LGG +++NAG Y+R +  F+ ++GV+WD++LV +AYEWVE+ +V +P   
Sbjct: 121 DGIDNVRSRILGGTTIINAGVYARANESFYNNSGVEWDLDLVNEAYEWVEDAIVYKPSNQ 180

Query: 181 AWQSAFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVA 240
           +WQS   ++ LE GV PDNGF L H  GT+  GS FDN G RH + ELLNK +P N+KVA
Sbjct: 181 SWQSITGTAFLEAGVHPDNGFGLVHEEGTRLTGSTFDNSGTRHASDELLNKGDPDNLKVA 240

Query: 241 IEATVQRILF----SGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGI 300
           +EA VQ+I+F    SGL+A GV+Y+DS G  HRA++  KGE+I+SAG +G+PQLLLLSG+
Sbjct: 241 VEAAVQKIIFSTESSGLTAVGVVYTDSNGTSHRALVSGKGEVILSAGTLGTPQLLLLSGV 300

Query: 301 GPKSHLSSLKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSI 360
           GP+S+L+SL + VV   PYVGQ ++DNPR   NI+ P P+ PS+V V+GI  D  + Q  
Sbjct: 301 GPESYLTSLNISVVASHPYVGQYVNDNPRNFINILPPNPIEPSTVTVLGITSD--FYQCS 360

Query: 361 ASPFPILIPQIFSLLPPQATSI-IPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFN 420
            S  P   P  FSL P  +  +   T A  V K     S GSL L SS+NV  +P V FN
Sbjct: 361 LSSLPFDTPP-FSLFPTTSYPLPNQTFAHIVSKVPGPLSAGSLTLQSSSNVSVAPNVKFN 420

Query: 421 YYSHPDDLGRCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEE 480
           Y S P DL  CV G++K+G  L T  ++  K  +L G  GF  LG PLPEN  +D++ E+
Sbjct: 421 YCSDPVDLTHCVSGMKKIGVFLSTDALKPYKVDDLPGIDGFNILGTPLPENQTDDAAFEK 480

Query: 481 YCKKTVTTYWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRY 540
           +C+ TV +YWHYHGG +VGKV+DGN++V GI  LRVVDGSTF  +P ++P    +MLGRY
Sbjct: 481 FCRDTVASYWHYHGGAIVGKVIDGNFRVTGINALRVVDGSTFPATPASHPQGFYLMLGRY 540

Query: 541 VGLKLLHQRS 542
           VG K++ +RS
Sbjct: 541 VGTKIVQERS 547

BLAST of CsGy2G005908 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 2.5e-151
Identity = 267/549 (48.63%), Postives = 374/549 (68.12%), Query Frame = 0

Query: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPN---QDVSYMKFVHNATDLPPKEEYDYIIIG 60
           M  S ++ ++L L I +  LQ     ++ N    D SY+ FV++ATD   +  YDYII+G
Sbjct: 1   MVKSTMSAVLLVLHIFVLHLQYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVG 60

Query: 61  GGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGRNPFQRFVSED 120
           GGTAGCPLA TLS+ +SVL+LERGS P +YP++L   G +      DDG+ P +RFVSED
Sbjct: 61  GGTAGCPLAATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSED 120

Query: 121 GVENIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNA 180
           G++N+RGRVLGG SM+NAG Y R +  FF   G++WDM+LV + YEWVE+T+V +P    
Sbjct: 121 GIDNVRGRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQT 180

Query: 181 WQSAFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAI 240
           WQ+   ++ LE G++P+NGF + HL GT+  GS FDN G RH + ELLNK +P N++VA+
Sbjct: 181 WQTVIGTAYLEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAV 240

Query: 241 EATVQRILF----SGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIG 300
           +A V++I+F    SG++A GV+Y+DS G  H+A +R +GE+I+SAG IGSPQLLLLSG+G
Sbjct: 241 QAAVEKIIFSSNTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVG 300

Query: 301 PKSHLSSLKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIA 360
           P+S+L+SL + VV   PYVGQ + DNPR   NI+ P P+  S+V V+GI  D  + Q   
Sbjct: 301 PESYLTSLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSD--FYQCSI 360

Query: 361 SPFPILIPQIFSLLPPQATSI-IPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNY 420
           S  P   P  FS  P  +  +   T A  V K     S G++ LNSS++V+  P V FNY
Sbjct: 361 SSLPFDTPP-FSFFPTTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNY 420

Query: 421 YSHPDDLGRCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEY 480
           YS+  DL  CV G++K+G++L T  +E  K ++L G  GF  LG+PLPEN  +D++ E +
Sbjct: 421 YSNLTDLSHCVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETF 480

Query: 481 CKKTVTTYWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYV 540
           C+++V +YWHYHGGCLVGKV+D  ++V GI  LRVVDGSTF  +P ++P    +MLGRY+
Sbjct: 481 CRESVASYWHYHGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYM 540

Query: 541 GLKLLHQRS 542
           G+++L +RS
Sbjct: 541 GIQILQERS 546

BLAST of CsGy2G005908 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 5.7e-151
Identity = 270/549 (49.18%), Postives = 368/549 (67.03%), Query Frame = 0

Query: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPN---QDVSYMKFVHNATDLPPKEEYDYIIIG 60
           ME S +++++  L + +  LQ    H++ N    D SY+KFV+NATD   +  YDYI+IG
Sbjct: 1   MEKSTMSVILFVLHLLVLHLQYSEVHSLANTSAHDFSYLKFVYNATDTSLEGSYDYIVIG 60

Query: 61  GGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGRNPFQRFVSED 120
           GGT+GCPLA TLS K+ VLLLERG+   +YP+ L   G        DDG+ P +RFVSED
Sbjct: 61  GGTSGCPLAATLSEKYKVLLLERGTIATEYPNTLTADGFAYNLQQQDDGKTPVERFVSED 120

Query: 121 GVENIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNA 180
           G++N+R R+LGG +++NAG Y+R +  F++  G++WD++LV K YEWVE+ +V +P   +
Sbjct: 121 GIDNVRARILGGTTIINAGVYARANISFYSQTGIEWDLDLVNKTYEWVEDAIVVKPNNQS 180

Query: 181 WQSAFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAI 240
           WQS      LE G++PDNGF L H  GT+  GS FDN G RH A ELLNK +P N+ VA+
Sbjct: 181 WQSVIGEGFLEAGILPDNGFSLDHEAGTRLTGSTFDNNGTRHAADELLNKGDPNNLLVAV 240

Query: 241 EATVQRILF----SGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIG 300
           +A+V++ILF    S LSA GV+Y+DS G  H+A +R  GE+IVSAG IG+PQLLLLSG+G
Sbjct: 241 QASVEKILFSSNTSNLSAIGVIYTDSDGNSHQAFVRGNGEVIVSAGTIGTPQLLLLSGVG 300

Query: 301 PKSHLSSLKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIA 360
           P+S+LSSL + VV   PYVGQ + +NPR   N   P P+  S V V+GI  D  Y Q   
Sbjct: 301 PESYLSSLNITVVQPNPYVGQFLYNNPRNFINNFPPNPIEASVVTVLGIRSD--YYQVSL 360

Query: 361 SPFPILIPQIFSLLPPQATSI-IPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNY 420
           S  P   P  FSL P  +  +   T A  V +     S GS+ LNSS++V+ +P + FNY
Sbjct: 361 SSLPFSTPP-FSLFPTTSYPLPNSTFAHIVSQVPGPLSHGSVTLNSSSDVRIAPNIKFNY 420

Query: 421 YSHPDDLGRCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEY 480
           YS+  DL  CV G++K+GDLL+T+ +E  K +++ G  GF +LGVPLPEN  +D+S E +
Sbjct: 421 YSNSTDLANCVSGMKKLGDLLRTKALEPYKARDVLGIDGFNYLGVPLPENQTDDASFETF 480

Query: 481 CKKTVTTYWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYV 540
           C   V +YWHYHGG LVGKV+D +++V+GIK LRVVD STF   P ++P    +MLGRYV
Sbjct: 481 CLDNVASYWHYHGGSLVGKVLDDSFRVMGIKALRVVDASTFPYEPNSHPQGFYLMLGRYV 540

Query: 541 GLKLLHQRS 542
           GL++L +RS
Sbjct: 541 GLQILQERS 546

BLAST of CsGy2G005908 vs. NCBI nr
Match: XP_011650116.2 ((R)-mandelonitrile lyase 1 [Cucumis sativus])

HSP 1 Score: 1078 bits (2788), Expect = 0.0
Identity = 543/544 (99.82%), Postives = 544/544 (100.00%), Query Frame = 0

Query: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGT 60
           MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGT
Sbjct: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGRNPFQRFVSEDGVE 120
           AGCPLATTLSSKFSVLLLERGSDPNKYPSVL+EQGLLNVFAAGDDGRNPFQRFVSEDGVE
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQS 180
           NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQS
Sbjct: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 181 AFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240
           AFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT
Sbjct: 181 AFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240

Query: 241 VQRILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSS 300
           VQRILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSS 300

Query: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILI 360
           LKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILI
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILI 360

Query: 361 PQIFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLG 420
           PQIFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLG
Sbjct: 361 PQIFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLG 420

Query: 421 RCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480
           RCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY
Sbjct: 421 RCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480

Query: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQR 540
           WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQR
Sbjct: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQR 540

Query: 541 SNLK 544
           SNLK
Sbjct: 541 SNLK 544

BLAST of CsGy2G005908 vs. NCBI nr
Match: KAE8651660.1 (hypothetical protein Csa_021193 [Cucumis sativus])

HSP 1 Score: 1057 bits (2733), Expect = 0.0
Identity = 530/531 (99.81%), Postives = 531/531 (100.00%), Query Frame = 0

Query: 14  MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKF 73
           MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKF
Sbjct: 1   MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKF 60

Query: 74  SVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMV 133
           SVLLLERGSDPNKYPSVL+EQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMV
Sbjct: 61  SVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMV 120

Query: 134 NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVP 193
           NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVP
Sbjct: 121 NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVP 180

Query: 194 DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG 253
           DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG
Sbjct: 181 DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG 240

Query: 254 VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG 313
           VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG
Sbjct: 241 VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG 300

Query: 314 QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSLLPPQATS 373
           QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSLLPPQATS
Sbjct: 301 QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSLLPPQATS 360

Query: 374 IIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL 433
           IIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL
Sbjct: 361 IIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL 420

Query: 434 KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV 493
           KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV
Sbjct: 421 KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV 480

Query: 494 DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQRSNLK 544
           DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQRSNLK
Sbjct: 481 DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQRSNLK 531

BLAST of CsGy2G005908 vs. NCBI nr
Match: XP_008452041.1 (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo])

HSP 1 Score: 996 bits (2576), Expect = 0.0
Identity = 501/543 (92.27%), Postives = 523/543 (96.32%), Query Frame = 0

Query: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGT 60
           MEH RVTILILSLMISIFQLQLHSS AIPNQDV YMKFVHNATDLP KEEYDYIIIGGGT
Sbjct: 1   MEHFRVTILILSLMISIFQLQLHSSLAIPNQDVHYMKFVHNATDLPTKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGRNPFQRFVSEDGVE 120
           AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDG+NPFQRFVSEDG+E
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGIE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQS 180
           NIR RVLGGGSMVNAGFYSRGH+EFFASAGVDW+MELVEKAYEWVEETVVSQPILNAWQS
Sbjct: 121 NIRARVLGGGSMVNAGFYSRGHQEFFASAGVDWNMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 181 AFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240
           AFRSSLLEGGV+PDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVA EAT
Sbjct: 181 AFRSSLLEGGVIPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVATEAT 240

Query: 241 VQRILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSS 300
           VQRILFSGLSANGVLYSDSKGKLH+A IR+KGEII+SAGAIGSPQLLLLSGIGPKS+LSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHKAFIRRKGEIILSAGAIGSPQLLLLSGIGPKSYLSS 300

Query: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILI 360
           LKLPVVLHQPYVGQSMSDNPRFGTNII+PFPVLPSSVKVVGILQDNIYIQSIASPFPIL+
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPFPILV 360

Query: 361 PQIFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLG 420
           P IFS L  Q TSI PT A+FVGKFSEVHSEGSLRLNSSTNVK+SPIV FNYYSHPDDLG
Sbjct: 361 PPIFSFLT-QVTSITPTFAIFVGKFSEVHSEGSLRLNSSTNVKRSPIVRFNYYSHPDDLG 420

Query: 421 RCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480
           RCVKGVRK+GDLLKT+TMEKIKT+NLEGNKGFEFLGVPLPENL N S+VEEYCKKT +TY
Sbjct: 421 RCVKGVRKIGDLLKTKTMEKIKTQNLEGNKGFEFLGVPLPENLGNVSAVEEYCKKTESTY 480

Query: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQR 540
           WHYHGGC+VGKVVDGNYKVIGIKNLR+VDGSTFSESPGTNPMATLMMLGRYVGLK+L +R
Sbjct: 481 WHYHGGCVVGKVVDGNYKVIGIKNLRIVDGSTFSESPGTNPMATLMMLGRYVGLKVLQER 540

Query: 541 SNL 543
            NL
Sbjct: 541 LNL 542

BLAST of CsGy2G005908 vs. NCBI nr
Match: KAA0067281.1 ((R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 978 bits (2529), Expect = 0.0
Identity = 492/530 (92.83%), Postives = 512/530 (96.60%), Query Frame = 0

Query: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGT 60
           MEH RVTILILSLMISIFQLQLHSS AIPNQDV YMKFVHNATDLP KEEYDYIIIGGGT
Sbjct: 1   MEHFRVTILILSLMISIFQLQLHSSLAIPNQDVHYMKFVHNATDLPTKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGRNPFQRFVSEDGVE 120
           AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDG+NPFQRFVSEDG+E
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGIE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQS 180
           NIR RVLGGGSMVNAGFYSRGH+EFFASAGVDW+MELVEKAYEWVEETVVSQPILNAWQS
Sbjct: 121 NIRARVLGGGSMVNAGFYSRGHQEFFASAGVDWNMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 181 AFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240
           AFRSSLLEGGV+PDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVA EAT
Sbjct: 181 AFRSSLLEGGVIPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVATEAT 240

Query: 241 VQRILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSS 300
           VQRILFSGLSANGVLYSDSKGKLH+A IR+KGEII+SAGAIGSPQLLLLSGIGPKS+LSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHKAFIRRKGEIILSAGAIGSPQLLLLSGIGPKSYLSS 300

Query: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILI 360
           LKLPVVLHQPYVGQSMSDNPRFGTNII+PFPVLPSSVKVVGILQDNIYIQSIASPFPIL+
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPFPILV 360

Query: 361 PQIFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLG 420
           P IFS L  Q TSI PT A+FVGKFSEVHSEGSLRLNSSTNVK+SPIV FNYYSHPDDLG
Sbjct: 361 PPIFSFLT-QVTSITPTFAIFVGKFSEVHSEGSLRLNSSTNVKRSPIVRFNYYSHPDDLG 420

Query: 421 RCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480
           RCVKGVRK+GDLLKT+TMEKIKT+NLEGNKGFEFLGVPLPENL N S+VEEYCKKT +TY
Sbjct: 421 RCVKGVRKIGDLLKTKTMEKIKTQNLEGNKGFEFLGVPLPENLGNVSAVEEYCKKTESTY 480

Query: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 530
           WHYHGGC+VGKVVDGNYKVIGIKNLR+VDGSTFSESPGTNPMATLMMLGR
Sbjct: 481 WHYHGGCVVGKVVDGNYKVIGIKNLRIVDGSTFSESPGTNPMATLMMLGR 529

BLAST of CsGy2G005908 vs. NCBI nr
Match: XP_038889315.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 959 bits (2480), Expect = 0.0
Identity = 478/542 (88.19%), Postives = 507/542 (93.54%), Query Frame = 0

Query: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGT 60
           MEHSR TILILSLMISIF L L SSHAIPNQDV YMKFVH+ATDLP KEEYDYIIIGGGT
Sbjct: 1   MEHSRSTILILSLMISIFHLGLLSSHAIPNQDVGYMKFVHSATDLPAKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGRNPFQRFVSEDGVE 120
           AGCPL++TLSSKFSVLLLERGSDPNKYPSVLNEQGLLN FAA DDG+NPFQ F+SEDGVE
Sbjct: 61  AGCPLSSTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFAAEDDGKNPFQHFISEDGVE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQS 180
           NIRGRVLGGGSMVN GFYSRGH++FF +AGVDWDMELVEKAY+WVEETVVSQPILNAWQS
Sbjct: 121 NIRGRVLGGGSMVNVGFYSRGHQQFFETAGVDWDMELVEKAYQWVEETVVSQPILNAWQS 180

Query: 181 AFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240
           AFRS+ L+ GVVPDNGFDLRH VGTK GGSIFDNKGNRHGAVELLNKANPTNIKV IEAT
Sbjct: 181 AFRSASLDAGVVPDNGFDLRHRVGTKIGGSIFDNKGNRHGAVELLNKANPTNIKVGIEAT 240

Query: 241 VQRILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSS 300
           VQRILFSGLSA GVLYSDSKGKLHRA IRK+GEIIVSAGA+GSPQLLLLSG+GPKSHLSS
Sbjct: 241 VQRILFSGLSARGVLYSDSKGKLHRAFIRKEGEIIVSAGAVGSPQLLLLSGVGPKSHLSS 300

Query: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILI 360
           LKLPVVLHQPYVG+ MSDNPRFGTNII+PF ++PSSVKVVGILQDNIYIQS ASP P   
Sbjct: 301 LKLPVVLHQPYVGEFMSDNPRFGTNIILPFQLVPSSVKVVGILQDNIYIQSFASPSPFFA 360

Query: 361 PQIFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLG 420
           P  FSLLPPQ  SI+P+LAMFVGKFSEVHSEGSLRLNSSTNVKKSPIV FNYYSHPDDL 
Sbjct: 361 PPSFSLLPPQFNSIVPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 420

Query: 421 RCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480
           RCVKGVRKMGDLLKT+TMEKIKT +LEGNKGF+FLG+PLPENL NDSSVEEYC+KTVTTY
Sbjct: 421 RCVKGVRKMGDLLKTQTMEKIKTHDLEGNKGFQFLGLPLPENLSNDSSVEEYCQKTVTTY 480

Query: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQR 540
           WHYHGGCLVGKVVDGNYKVIGI+NLRVVDGSTF +SPGTNPMATLMMLGRYVGLK+L QR
Sbjct: 481 WHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFCDSPGTNPMATLMMLGRYVGLKVLQQR 540

Query: 541 SN 542
           S+
Sbjct: 541 SS 542

BLAST of CsGy2G005908 vs. ExPASy TrEMBL
Match: A0A0A0LK76 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055000 PE=3 SV=1)

HSP 1 Score: 1048 bits (2711), Expect = 0.0
Identity = 528/530 (99.62%), Postives = 530/530 (100.00%), Query Frame = 0

Query: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGT 60
           MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGT
Sbjct: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGRNPFQRFVSEDGVE 120
           AGCPLATTLSSKFSVLLLERGSDPNKYPSVL+EQGLLNVFAAGDDGRNPFQRFVSEDGVE
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQS 180
           NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQS
Sbjct: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 181 AFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240
           AFRSSLLEGGVVPD+GFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT
Sbjct: 181 AFRSSLLEGGVVPDSGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240

Query: 241 VQRILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSS 300
           VQRILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSS 300

Query: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILI 360
           LKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILI
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILI 360

Query: 361 PQIFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLG 420
           PQIFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLG
Sbjct: 361 PQIFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLG 420

Query: 421 RCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480
           RCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY
Sbjct: 421 RCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480

Query: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 530
           WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR
Sbjct: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 530

BLAST of CsGy2G005908 vs. ExPASy TrEMBL
Match: A0A1S3BSB5 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493164 PE=3 SV=1)

HSP 1 Score: 996 bits (2576), Expect = 0.0
Identity = 501/543 (92.27%), Postives = 523/543 (96.32%), Query Frame = 0

Query: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGT 60
           MEH RVTILILSLMISIFQLQLHSS AIPNQDV YMKFVHNATDLP KEEYDYIIIGGGT
Sbjct: 1   MEHFRVTILILSLMISIFQLQLHSSLAIPNQDVHYMKFVHNATDLPTKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGRNPFQRFVSEDGVE 120
           AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDG+NPFQRFVSEDG+E
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGIE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQS 180
           NIR RVLGGGSMVNAGFYSRGH+EFFASAGVDW+MELVEKAYEWVEETVVSQPILNAWQS
Sbjct: 121 NIRARVLGGGSMVNAGFYSRGHQEFFASAGVDWNMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 181 AFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240
           AFRSSLLEGGV+PDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVA EAT
Sbjct: 181 AFRSSLLEGGVIPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVATEAT 240

Query: 241 VQRILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSS 300
           VQRILFSGLSANGVLYSDSKGKLH+A IR+KGEII+SAGAIGSPQLLLLSGIGPKS+LSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHKAFIRRKGEIILSAGAIGSPQLLLLSGIGPKSYLSS 300

Query: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILI 360
           LKLPVVLHQPYVGQSMSDNPRFGTNII+PFPVLPSSVKVVGILQDNIYIQSIASPFPIL+
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPFPILV 360

Query: 361 PQIFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLG 420
           P IFS L  Q TSI PT A+FVGKFSEVHSEGSLRLNSSTNVK+SPIV FNYYSHPDDLG
Sbjct: 361 PPIFSFLT-QVTSITPTFAIFVGKFSEVHSEGSLRLNSSTNVKRSPIVRFNYYSHPDDLG 420

Query: 421 RCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480
           RCVKGVRK+GDLLKT+TMEKIKT+NLEGNKGFEFLGVPLPENL N S+VEEYCKKT +TY
Sbjct: 421 RCVKGVRKIGDLLKTKTMEKIKTQNLEGNKGFEFLGVPLPENLGNVSAVEEYCKKTESTY 480

Query: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQR 540
           WHYHGGC+VGKVVDGNYKVIGIKNLR+VDGSTFSESPGTNPMATLMMLGRYVGLK+L +R
Sbjct: 481 WHYHGGCVVGKVVDGNYKVIGIKNLRIVDGSTFSESPGTNPMATLMMLGRYVGLKVLQER 540

Query: 541 SNL 543
            NL
Sbjct: 541 LNL 542

BLAST of CsGy2G005908 vs. ExPASy TrEMBL
Match: A0A5A7VNU1 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold418G001020 PE=3 SV=1)

HSP 1 Score: 978 bits (2529), Expect = 0.0
Identity = 492/530 (92.83%), Postives = 512/530 (96.60%), Query Frame = 0

Query: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGT 60
           MEH RVTILILSLMISIFQLQLHSS AIPNQDV YMKFVHNATDLP KEEYDYIIIGGGT
Sbjct: 1   MEHFRVTILILSLMISIFQLQLHSSLAIPNQDVHYMKFVHNATDLPTKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGRNPFQRFVSEDGVE 120
           AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDG+NPFQRFVSEDG+E
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGIE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQS 180
           NIR RVLGGGSMVNAGFYSRGH+EFFASAGVDW+MELVEKAYEWVEETVVSQPILNAWQS
Sbjct: 121 NIRARVLGGGSMVNAGFYSRGHQEFFASAGVDWNMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 181 AFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240
           AFRSSLLEGGV+PDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVA EAT
Sbjct: 181 AFRSSLLEGGVIPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVATEAT 240

Query: 241 VQRILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSS 300
           VQRILFSGLSANGVLYSDSKGKLH+A IR+KGEII+SAGAIGSPQLLLLSGIGPKS+LSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHKAFIRRKGEIILSAGAIGSPQLLLLSGIGPKSYLSS 300

Query: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILI 360
           LKLPVVLHQPYVGQSMSDNPRFGTNII+PFPVLPSSVKVVGILQDNIYIQSIASPFPIL+
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPFPILV 360

Query: 361 PQIFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLG 420
           P IFS L  Q TSI PT A+FVGKFSEVHSEGSLRLNSSTNVK+SPIV FNYYSHPDDLG
Sbjct: 361 PPIFSFLT-QVTSITPTFAIFVGKFSEVHSEGSLRLNSSTNVKRSPIVRFNYYSHPDDLG 420

Query: 421 RCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480
           RCVKGVRK+GDLLKT+TMEKIKT+NLEGNKGFEFLGVPLPENL N S+VEEYCKKT +TY
Sbjct: 421 RCVKGVRKIGDLLKTKTMEKIKTQNLEGNKGFEFLGVPLPENLGNVSAVEEYCKKTESTY 480

Query: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 530
           WHYHGGC+VGKVVDGNYKVIGIKNLR+VDGSTFSESPGTNPMATLMMLGR
Sbjct: 481 WHYHGGCVVGKVVDGNYKVIGIKNLRIVDGSTFSESPGTNPMATLMMLGR 529

BLAST of CsGy2G005908 vs. ExPASy TrEMBL
Match: A0A1S3BS35 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493093 PE=3 SV=1)

HSP 1 Score: 956 bits (2471), Expect = 0.0
Identity = 479/530 (90.38%), Postives = 499/530 (94.15%), Query Frame = 0

Query: 14  MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKF 73
           MISIFQLQLHSSHAIPNQDVSYMKFVHNATDL  KEEYDYIIIGGGTAGCPLATTLSSKF
Sbjct: 1   MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLSTKEEYDYIIIGGGTAGCPLATTLSSKF 60

Query: 74  SVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMV 133
           SVL+LERGSDPNKYP VLNE GL NVF AGDDG+NP QRF+SEDGVENIRGRVLGGGSMV
Sbjct: 61  SVLVLERGSDPNKYPFVLNELGLFNVFTAGDDGKNPSQRFISEDGVENIRGRVLGGGSMV 120

Query: 134 NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVP 193
           NAGFYSRGHREFF SAGVDWDMELVEKAYEWVEETVVSQPILNAWQS FRSSLLE GVVP
Sbjct: 121 NAGFYSRGHREFFESAGVDWDMELVEKAYEWVEETVVSQPILNAWQSTFRSSLLESGVVP 180

Query: 194 DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG 253
           DNGFDLRHLVGTKTGGSIFDN GNRHGAVE LNKAN TNIKVAIEATVQRILFSGLSANG
Sbjct: 181 DNGFDLRHLVGTKTGGSIFDNIGNRHGAVEFLNKANTTNIKVAIEATVQRILFSGLSANG 240

Query: 254 VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG 313
           VLYSDSKGKLH+A IRKKGEII+SAGAIGSPQLLLLSGIGPKS+LSSLKLP+VLHQPYVG
Sbjct: 241 VLYSDSKGKLHKAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSYLSSLKLPLVLHQPYVG 300

Query: 314 QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSLLPPQATS 373
           QSMSDNPRFGT  I+PFPVLPSS K VGILQDNIYIQSIAS  PILIP IFS LPP ATS
Sbjct: 301 QSMSDNPRFGTYFILPFPVLPSSAKAVGILQDNIYIQSIASLLPILIPPIFSFLPPGATS 360

Query: 374 IIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL 433
           I P+L + +GKFSEVHSEGSLRLNSST+VK+SPIV FNYYSHPDDLGRCVKGVRK+GDLL
Sbjct: 361 IDPSLVVLIGKFSEVHSEGSLRLNSSTDVKRSPIVRFNYYSHPDDLGRCVKGVRKIGDLL 420

Query: 434 KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV 493
           KT+TMEKIKT+NLE NKGFEFLGVPLPENL N S+VEEYCKKTV+TYWHYHGGC+VGKVV
Sbjct: 421 KTKTMEKIKTQNLEDNKGFEFLGVPLPENLGNVSAVEEYCKKTVSTYWHYHGGCVVGKVV 480

Query: 494 DGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQRSNL 543
           DGNYKVIGIKNLR+VDGSTFSESPGTNPMATLMMLGRYVGLKLL QRSNL
Sbjct: 481 DGNYKVIGIKNLRIVDGSTFSESPGTNPMATLMMLGRYVGLKLLQQRSNL 530

BLAST of CsGy2G005908 vs. ExPASy TrEMBL
Match: A0A5A7VGR6 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold418G001190 PE=3 SV=1)

HSP 1 Score: 954 bits (2467), Expect = 0.0
Identity = 480/530 (90.57%), Postives = 500/530 (94.34%), Query Frame = 0

Query: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGT 60
           MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDL  KEEYDYIIIGGGT
Sbjct: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLSTKEEYDYIIIGGGT 60

Query: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGRNPFQRFVSEDGVE 120
           AGCPLATTLSSKFSVL+LERGSDPNKYP VLNE GL NVF AGDDG+NP QRF+SEDGVE
Sbjct: 61  AGCPLATTLSSKFSVLVLERGSDPNKYPFVLNELGLFNVFTAGDDGKNPSQRFISEDGVE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQS 180
           NIRGRVLGGGSMVNAGFYSRGHREFF SAGVDWDMELVEKAYEWVEETVVSQPILNAWQS
Sbjct: 121 NIRGRVLGGGSMVNAGFYSRGHREFFESAGVDWDMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 181 AFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240
            FRSSLLE GVVPDNGFDLRHLVGTKTGGSIFDN GNRHGAVE LNKAN TNIKVAIEAT
Sbjct: 181 TFRSSLLESGVVPDNGFDLRHLVGTKTGGSIFDNIGNRHGAVEFLNKANTTNIKVAIEAT 240

Query: 241 VQRILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSS 300
           VQRILFSGLSANGVLYSDSKGKLH+A IRKKGEII+SAGAIGSPQLLLLSGIGPKS+LSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHKAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSYLSS 300

Query: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILI 360
           LKLP+VLHQPYVGQSMSDNPRFGT  I+PFPVLPSS K VGILQDNIYIQSIAS  PILI
Sbjct: 301 LKLPLVLHQPYVGQSMSDNPRFGTYFILPFPVLPSSAKAVGILQDNIYIQSIASLLPILI 360

Query: 361 PQIFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLG 420
           P IFS LPP ATSI P+L + +GKFSEVHSEGSLRLNSST+VK+SPIV FNYYSHPDDLG
Sbjct: 361 PPIFSFLPPGATSIDPSLVVLIGKFSEVHSEGSLRLNSSTDVKRSPIVRFNYYSHPDDLG 420

Query: 421 RCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480
           RCVKGVRK+GDLLKT+TMEKIKT+NLE NKGFEFLGVPLPENL N S+VEEYCKKTV+TY
Sbjct: 421 RCVKGVRKIGDLLKTKTMEKIKTQNLEDNKGFEFLGVPLPENLGNVSAVEEYCKKTVSTY 480

Query: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 530
           WHYHGGC+VGKVVDGNYKVIGIKNLR+VDGSTFSESPGTNPMATLMMLGR
Sbjct: 481 WHYHGGCVVGKVVDGNYKVIGIKNLRIVDGSTFSESPGTNPMATLMMLGR 530

BLAST of CsGy2G005908 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 525.8 bits (1353), Expect = 4.2e-149
Identity = 263/544 (48.35%), Postives = 369/544 (67.83%), Query Frame = 0

Query: 7   TILILSLMISIFQLQLHSSHAIP--NQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCP 66
           ++L  +L++ +    +H S+A P  N+   +M+F+ NATD   ++ YDYII+GGGTAGCP
Sbjct: 8   SLLYTALVVLLLLGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAGCP 67

Query: 67  LATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRG 126
           LA TLS  F VLLLERG  P   P+V++  G L      ++  +P Q F+SE+GV N RG
Sbjct: 68  LAATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNARG 127

Query: 127 RVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRS 186
           RVLGG S +NAGFYSR  ++FF ++G+ WD+  V ++YEWVE  +V +P L  WQ+A R 
Sbjct: 128 RVLGGSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRD 187

Query: 187 SLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRI 246
           +LLE GV P NGF L H VGTK GGS FD  G RH + +LL  A  +NI+VA+ ATV+R+
Sbjct: 188 ALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVERV 247

Query: 247 LF--------SGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKS 306
           L         S +SA GV+Y D  G+ H A+IR +GE+I+SAGA+GSPQLL LSGIGP+S
Sbjct: 248 LLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRS 307

Query: 307 HLSSLKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPF 366
           +LS+  +PV L QP+VG  + DNPR G +I+ P P+  S ++VVG+ +D  ++++ ++  
Sbjct: 308 YLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVI 367

Query: 367 PILIPQIFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHP 426
           P   P     +   A+ +   +   + K     S G LRL +ST+V+ +P+V FNY+S P
Sbjct: 368 PFASPLHSVFIRAPASPLYVPVTTIMEKILGPVSIGLLRL-ASTDVRINPVVRFNYFSDP 427

Query: 427 DDLGRCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKT 486
            DL RCV G RK+G++L++R M+    +   GN+ F F+G PLP +  ND  + ++C++T
Sbjct: 428 QDLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFCRRT 487

Query: 487 VTTYWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKL 541
           V+T WHYHGG +VGKVVD + KVIG+ +LR+VDGSTF+ SPGTNP ATLMMLGRY+GLK+
Sbjct: 488 VSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGRYMGLKM 547

BLAST of CsGy2G005908 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 426.0 bits (1094), Expect = 4.5e-119
Identity = 253/578 (43.77%), Postives = 337/578 (58.30%), Query Frame = 0

Query: 11  LSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLS 70
           L + +S+F   LHS     ++  +Y  F+ +AT  P    YDYIIIGGGTAGCPLA TLS
Sbjct: 10  LFVALSLF---LHSPICSSDKAPNY-SFMRDATGSPTTSYYDYIIIGGGTAGCPLAATLS 69

Query: 71  SKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDG---RNPFQRFVSEDGVENIRGRVL 130
              SVLLLERG  P   P++      L+ F A        +P QRFVSEDGV N R RVL
Sbjct: 70  QNASVLLLERGDSPYNNPNITR----LSAFGAALSDLSESSPSQRFVSEDGVINARARVL 129

Query: 131 GGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLL 190
           GGGS +NAGFY+R   ++  + G  WD  L  ++Y+WVE  V  QP +  WQ+A R  LL
Sbjct: 130 GGGSALNAGFYTRAGTKYVRNMG--WDGALANESYQWVEAKVAFQPPMGRWQTAVRDGLL 189

Query: 191 EGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFS 250
           E G+VP+NGF   H+ GTK GG+IFD  GNRH A +LL  A+P  I V + ATV RILF 
Sbjct: 190 EAGIVPNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILFR 249

Query: 251 GLS-----ANGVLYSDSKGKLHRAIIRK--KGEIIVSAGAIGSPQLLLLSGIGPKSHLSS 310
                   ANGV+Y D  G+ HRA +++    EII+SAG +GSPQLL+LSG+GP + L +
Sbjct: 250 TRGTTKPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQA 309

Query: 311 LKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSI-------- 370
             + VV+ QP+VGQ M DNP     +  P PV  S ++VVGI  +  Y+++         
Sbjct: 310 QNITVVMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGG 369

Query: 371 --ASPFPILIPQIFSLLPPQATSI-------IPTLAMFVG-----KFSEVHSEGSLRLNS 430
              S         +++  P+AT +       + +   F G     K     S G L L  
Sbjct: 370 GGGSSGSSSTRDYYAMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLEL-K 429

Query: 431 STNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEF---L 490
           + N K +PIV FNY+ HPDDL RCV+G++ +  +++++   + K  ++     FE+   L
Sbjct: 430 TRNPKDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADV----SFEYLLNL 489

Query: 491 GVPLPENLWN---------DSSVEEYCKKTVTTYWHYHGGCLVGKVVDGNYKVIGIKNLR 545
               P NL             S EE+C+ TVTT WHYHGGC+VG+VVDG+YKVIGI  LR
Sbjct: 490 TASTPVNLRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLR 549

BLAST of CsGy2G005908 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 412.5 bits (1059), Expect = 5.2e-115
Identity = 244/539 (45.27%), Postives = 324/539 (60.11%), Query Frame = 0

Query: 51  YDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDP--NKYPSVLNEQGLLNVFAAGDDGRN 110
           YDYI+IGGGTAGCPLA TLS  FSVL+LERG  P  N   S L      ++  A     +
Sbjct: 63  YDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRN---FHIGLADISASS 122

Query: 111 PFQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEET 170
             Q FVS DGV N R RVLGGGS +NAGFYSR    F   AG  WD +LV+++Y WVE  
Sbjct: 123 ASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPWVERE 182

Query: 171 VVSQPILNAWQSAFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKA 230
           +V QP L  WQ A R SLLE GV P NGF   H+ GTK GG+IFD  G RH A ELL  A
Sbjct: 183 IVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYA 242

Query: 231 NPTNIKVAIEATVQRILF--SGL--SANGVLYSDSKGKLHRAII--RKKGEIIVSAGAIG 290
           NP  ++V I ATVQ+I+F  SG      GV++ D KG  H+A++  RK  E+I+S+GAIG
Sbjct: 243 NPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIG 302

Query: 291 SPQLLLLSGIGPKSHLSSLKLPVVLHQPYVGQSMSDNPRFGTNIIIP--FPVLPSSVKVV 350
           SPQ+L+LSGIGPK  L  LK+PVVL   +VG+ M+DNP     I++P   P+  S ++ V
Sbjct: 303 SPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPM--NTILVPSKAPIEQSLIQTV 362

Query: 351 GILQDNIYI----------QSIASPFPILI--PQIFSLLP-----PQATSIIPTL----- 410
           GI +  +Y+          +SI + + I+    ++FS +P     P+AT    T      
Sbjct: 363 GITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQL 422

Query: 411 ------AMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDL 470
                 +  + K +   S G L L  +TNV  +P V FNY+ HP DL RCV+ +R +  +
Sbjct: 423 HEAFNGSFILEKLAYPISRGHLSL-VNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVSKV 482

Query: 471 LKT-----------RTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYW 530
           + +           + + K+ + +++ N          P+ L +  S+ ++CK TV T W
Sbjct: 483 VTSNRFLNYTQCDKQNVHKMLSLSVKANINLR------PKQLNDTKSMAQFCKDTVVTIW 542

Query: 531 HYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQR 541
           HYHGGCLVGKVV  N KV+G+  LRV+DGSTF ESPGTNP AT+MM+GRY+G+K+L +R
Sbjct: 543 HYHGGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRER 587

BLAST of CsGy2G005908 vs. TAIR 10
Match: AT3G56060.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 405.2 bits (1040), Expect = 8.2e-113
Identity = 239/541 (44.18%), Postives = 321/541 (59.33%), Query Frame = 0

Query: 37  KFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGL 96
           +F+ +AT  P    +DYIIIGGGTAGC LA TLS   +VL+LERG  P   P+  +    
Sbjct: 32  RFMKDATLAPKLSHFDYIIIGGGTAGCALAATLSQNATVLVLERGGSPYDDPAATDIGNF 91

Query: 97  LNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDME 156
            N         +  Q F+SEDGV N R RVLGGG+++NAGFYSR   +F A AG  W+ +
Sbjct: 92  ANTL-LNITPNSWSQLFISEDGVFNSRARVLGGGTVINAGFYSRAEEDFVAEAG--WERD 151

Query: 157 LVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKG 216
            VE AYEWVE+ VV +P +N WQSAFR  LLE GV P NGF   H+VGTK GG+IFD  G
Sbjct: 152 EVEAAYEWVEKKVVFEPPVNKWQSAFRDGLLEAGVTPYNGFTYEHIVGTKFGGTIFDRDG 211

Query: 217 NRHGAVELLNKANPTNIKVAIEATVQRILFS-----GLSANGVLYSDSKGKLHRAIIRKK 276
           +RH A  LL  ANP  I V + A+V +ILF+        A GV++ D+ G  ++A +  +
Sbjct: 212 HRHTAANLLEYANPNMIVVYLHASVHKILFTIKGNQRPKAYGVIFLDANGVSYKAELATQ 271

Query: 277 ----GEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKL-PVVLHQPYVGQSMSDNPRFGTNI 336
                E+I+SAGAI SPQLL+LSG+GP +HL++ ++ PV++ QP VGQ M DNP     I
Sbjct: 272 DSTMSEVILSAGAIASPQLLMLSGVGPAAHLAAYRVNPVIVDQPMVGQGMGDNPMNPVFI 331

Query: 337 IIPFPVLPSSVKVVGILQDNIYIQ-SIASPFPILIPQIF----------SLLPPQATS-- 396
             P PV  S V+ VGI +   YI+   A    I + + F          + LP Q+ S  
Sbjct: 332 PSPEPVEVSLVQAVGITKFGSYIEGGSALSLSISLTRSFFDGVLNLLKKTKLPTQSISKF 391

Query: 397 ---------IIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVK 456
                    +     + + K +   S G L L  +TN   +P V FNY+  P+DL +CV+
Sbjct: 392 FKSLDLTLNVTTKAGVIIQKVNGPLSRGHLELR-NTNPDDNPSVTFNYFKDPEDLNKCVE 451

Query: 457 GVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENL-----WNDSSVEEYCKKTVTT 516
           G+  +  ++ ++   K K   L   +G   L + LP NL      +   +E+YC  TV T
Sbjct: 452 GLSTIIKVIDSKGYSKYKYP-LASARGLLNLILALPTNLRPRHITSTFDLEQYCIDTVMT 511

Query: 517 YWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQ 541
            +HYHGGC VGKVVD NYKV+G+  LR++DGSTF +SPGTNP AT+MMLGRY+G K+L +
Sbjct: 512 IYHYHGGCQVGKVVDNNYKVLGVDALRIIDGSTFLKSPGTNPQATIMMLGRYMGQKILRE 567

BLAST of CsGy2G005908 vs. TAIR 10
Match: AT1G72970.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 398.7 bits (1023), Expect = 7.7e-111
Identity = 239/528 (45.27%), Postives = 313/528 (59.28%), Query Frame = 0

Query: 51  YDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDP--NKYPSVLNEQGLLNVFAAGDDGRN 110
           YDYI+IGGGTAGCPLA TLS  FSVL+LERG  P  N   S L      ++  A     +
Sbjct: 63  YDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRN---FHIGLADISASS 122

Query: 111 PFQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEET 170
             Q FVS DGV N R RVLGGGS +NAGFYSR    F   AG  WD +LV+++Y WVE  
Sbjct: 123 ASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPWVERE 182

Query: 171 VVSQPILNAWQSAFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKA 230
           +V QP L  WQ A R SLLE GV P NGF   H+ GTK GG+IFD  G RH A ELL  A
Sbjct: 183 IVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYA 242

Query: 231 NPTNIKVAIEATVQRILF--SGL--SANGVLYSDSKGKLHRAII--RKKGEIIVSAGAIG 290
           NP  ++V I ATVQ+I+F  SG      GV++ D KG  H+A++  RK  E+I+S+GAIG
Sbjct: 243 NPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIG 302

Query: 291 SPQLLLLSGIGPKSHLSSLKLPVVLHQPYVGQSMSDNPRFGTNIIIP--FPVLPSSVKVV 350
           SPQ+L+LSGIGPK  L  LK+PVVL   +VG+ M+DNP     I++P   P+  S ++ V
Sbjct: 303 SPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPM--NTILVPSKAPIEQSLIQTV 362

Query: 351 GILQDNIYI----------QSIASPFPILI--PQIFSLLP-----PQATSIIPTL----- 410
           GI +  +Y+          +SI + + I+    ++FS +P     P+AT    T      
Sbjct: 363 GITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQL 422

Query: 411 ------AMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDL 470
                 +  + K +   S G L L  +TNV  +P V FNY+ HP D              
Sbjct: 423 HEAFNGSFILEKLAYPISRGHLSL-VNTNVDDNPSVTFNYFKHPCD-------------- 482

Query: 471 LKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKV 530
              + + K+ + +++ N          P+ L +  S+ ++CK TV T WHYHGGCLVGKV
Sbjct: 483 --KQNVHKMLSLSVKANINLR------PKQLNDTKSMAQFCKDTVVTIWHYHGGCLVGKV 542

Query: 531 VDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQR 541
           V  N KV+G+  LRV+DGSTF ESPGTNP AT+MM+GRY+G+K+L +R
Sbjct: 543 VSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRER 560

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P527068.7e-16051.46(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1[more]
Q945K22.4e-15750.82(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1[more]
O827842.0e-15149.45(R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1[more]
P527072.5e-15148.63(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1[more]
O242435.7e-15149.18(R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_011650116.20.099.82(R)-mandelonitrile lyase 1 [Cucumis sativus][more]
KAE8651660.10.099.81hypothetical protein Csa_021193 [Cucumis sativus][more]
XP_008452041.10.092.27PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo][more]
KAA0067281.10.092.83(R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa][more]
XP_038889315.10.088.19(R)-mandelonitrile lyase 1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A0A0LK760.099.62Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055000 PE=3 SV=1[more]
A0A1S3BSB50.092.27(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493164 PE=3 SV=... [more]
A0A5A7VNU10.092.83(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S3BS350.090.38(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493093 PE=3 SV=... [more]
A0A5A7VGR60.090.57(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
Match NameE-valueIdentityDescription
AT1G73050.14.2e-14948.35Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G12570.14.5e-11943.77Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.15.2e-11545.27Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT3G56060.18.2e-11344.18Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.27.7e-11145.27Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.410.40coord: 171..479
e-value: 5.8E-170
score: 568.4
NoneNo IPR availablePANTHERPTHR45968OSJNBA0019K04.7 PROTEINcoord: 4..542
NoneNo IPR availablePANTHERPTHR45968:SF23(R)-MANDELONITRILE LYASE 4-RELATEDcoord: 4..542
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 389..486
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 51..529
e-value: 5.8E-170
score: 568.4
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 32..538
IPR012132Glucose-methanol-choline oxidoreductasePIRSFPIRSF000137Alcohol_oxidasecoord: 353..544
e-value: 2.3E-30
score: 103.8
coord: 31..341
e-value: 2.8E-56
score: 189.4
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 51..320
e-value: 5.6E-31
score: 108.0
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00623GMC_OXRED_1coord: 124..147
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00624GMC_OXRED_2coord: 279..293
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 389..530
e-value: 2.6E-28
score: 99.3

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy2G005908.1CsGy2G005908.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors