CsGy2G005902 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy2G005902
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description(R)-mandelonitrile lyase 1-like
LocationGy14Chr2: 4176037 .. 4180586 (-)
RNA-Seq ExpressionCsGy2G005902
SyntenyCsGy2G005902
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCACTCAACCATGTCTGCCTTTCTTCTTCACATCCTTATTTTCTGCTGTCAATCTCATTTAGGAGTCTTCTCCACCAGTTACACTAAACTCGGTAATAGTGATCAAGGTAAAACTAATAGCACTCTCTTCATATATATATGTAATCCACTTCTTTCTCTCTCTCTCTTTTATTAGTTCTAAACTTTGGTAATGAAAGTTGTCGAGAAGTATCAAATAACCTAGTTGACATGTATCAGTTCACCTTCTCTTTTTTTTTTTTATTGTGAATATTTTCAAAACAAGAAAAAAAATTGGTTCTAAACTTTTCTTCAGATGTCAGCTACATGAAGTTTGTGCACAATGCAAATAAGTTCCCAGAAAAAGAAGAATATGACTACATAATAATAGGAGGAGGAACTGCAGGCTGTCCATTAGCTGCAACATTATCATCAAAATTCTCCGTCCTCCTCTTAGAAAGAGGCAATATTCCCAACAAGTTTCCTTCTGTATTGAACAAACAAGGATTGATGAATGCTTTCACTGACAAAGACGATGGTGAAAATCCCTTCCAACGGTTCGTTTCCGAGGATGGCGTAGAGAATCTAAGAGGGCGGATTTTGGGTGGTAGTAGCATGATCAATGCTGGATTCTATTCAAGGGCTCATAAAGAGTTTTTTGAAACACAAGAAATAATTGAATGGGACATGGAAATGGTGAGAGAAGCTTATGAATGGGTTGAAGAGACTTTGGTTTCTGAACCAAATTTGAGTTCTTGGCAATTTGCTTTTAGAAAGGCATTGTTGGAAGTTGGGGTTGACCATGATAATGGGTTTGAGTTGAGACATCTTGTAGGGACTAAAATTGGAGGTTCTATCTTTGATAACCAAGGCAATAGACATGGAGCTGTAGAGCTTCTTAATAAGGGAGAATCTGAAAATCTTAAAGTTGCAGTTCAGGCTACAGTCAAAAGAATTCTCTTCTCTGGTTAGTACTAACTGATTTTGTTACTCTTTTAATTAAGTTGCTTCTCAATAGCATATTTTGTCTGTGAGCTCCCTTCAAGCTCCTGTTCATCGATCTAGATACAAAGTTTGATGAGGGTGTGCAATGTGTCAACCTTTGTTGAAGCGTCTAGACACACCTCCTAGTCTCTCCCATATGTGTATATCTTATTAAAAAAAATGTTAGTTTTCAAATATAACAAAATTAACTAAAGTATTTATGAATATAAAAAAATATTAATGATAAGTATTGATAGATACTGATAGACGACTATTATCGTCACTTATCATTGATATATATTAATAGACACTAATAAATGTTTATTAATATTTATCAATGTCTAAAAATTGAGAATGATGTGGGTACAATGAAGGTGCCCACCTACAGTATTATTTTCTAAAAATTTATTTATCAATATCTATAACTAATAGCTATGAAATTTTATCTGTTTTAAAATATTTTCACCAATTTTGTTACATACGCCCATTTTCAAAAACACATATCAATTGTTAAAATTAAAATTATACCCAAACATTTTCAAACTTGTTATGAAGAAGTGATATCTAAGGGAAAAAAGGTCCAACCTATTAAACTAAACCAAGATATGTAAAGAAGTATATAATATATGACATCAAATAATTAATATATGCTTTACTTTTGATCATGTGTTGTATTACCTAGGTTTGAAAATGGGTGATCGAAATATTATTTAAAACAAAACCCAAAAAATTATGAAAAGAAAAGGGAATTTTTCAAAATAGAATAAATGAGCTAAATATTTACATATATAGAACAATTTGAAAATCGAAAAATACTCACAACCCCACAATGTAAAATTCCAAAAATGTCTCATGATTAATTGTTATCTAGAACACGTAATATATTTGGGAAACGATCGTTTAAATTTGGTTATTCTCTCATGTAATCATTTAGATTTGATAGTTTAGATTTGAGTTTGGCGAATTTTTTCAAGATTTTTTGGTCTGCAATCATTTAGATTTGGTACACGATCGTTTAATTTAATCGTTTAAATTTTGGTAGCAAAATTAAAACGATTTTTTTAGATATTTTTGTACTTCAATAGTTTAGAATTAGCATATGATCGTTTAGATTTGACACATGAACATTTAAATTTGGCAATTTGATCATTTAAATTAGGGTAGCCAAATCTCAACTTTTCTTTTTCAAGATTTTTTGGTACACTATTGTTTATATTTAGTACATCATTATTTAGATTTGGCTACTTTTGTACATTATCGTTTGCATGACAATCCAATATCCTAACGATTTTTTTCACGATATTTTATATTTGACACATGATCTTGAACCAAATAACAGATTGAAAAAATATAAAAGAATTATTGAAGAAAGAAAAAGACGATGAAAAGGAAAAGAAAAAAAATTACAGAAGAAGAGGAAAAGATGATAGGAAGGAAAAGAAAAATTACAGAAGAAAGAATATGAAAAGGAAAATATTTTTAAAAAATTGGAGGAAAAAAGAAAAAGACAATGGAAAGAAAAAATATCGCCGAAAAGGAAAAGAAAAAAAATCGCAGAAGAGAGGAGAAGAAAGACGATGATAAGAAAGAGCAAACCTAGATATTTTTTAAAAAATGATAAACTTCATGAGCTTTTTCAATTTGTGGCACGGGCCGTAAATACATTTTTTTCCGTTTGTTGTATTCATGAAAGTAAGATATTTGATAGGGGAAAATGTCAACATAAAACGATTTTTAAATTGAATTTGAATATGCTTTGACTTATCATTTGTGCTCTCATAATTCGAAGGCTTGCTATACCTAACAAAGTTTATAAACATAGTGGTTGGTTTGAATCTATGTTTGGCTAGCTAATTATATACCATAAAACTTTTTTAAAAAATTGGAATAAAAAATCAATAATTTTATAATTATTGAAATTTGATGATATGTATCATGCATATATGTCTAACTTGTAGCTCGGTTTGGATTGTTGGTACATTTTTTAATAATTGTTCAAAATATTGTTCAACAAATTTGGATTGTTGTGATATAAAATAAGTTGATGAGTATACCACGCATATATATGTTTATTTGGTTGAGATGTTTGTGTGAATCTACTCAACTCTCTCCATACTATTTACATGAATTTTGTAGGTTTATCTGCAAATGGGGTTTTGTATTCAGATTCGAAAGGAAAGTCACACACAGCATTCATCCATGAGAAAGGAGAGATAATTCTAAGTGCTGGAGCCATTGGTAGCCCTCAACTTCTCCTTCTAAGTGGGGTTGGCTCAACATCTCATCTTTCTTCTTTAAACCTTCCTCTCTTCCTTCACCAACCCCATGTCGGACAATTCATGTCGGACAACCCTCGATTTGGCGTCAATATTGTGCTTCCATTTCCTCTACCAACGACGACGGTGGAAGTCGTCGGAATTTTAGAAAAGAATACATATTTTGAGTCTTTGTCAAGCTTTATACCATTCTCAATTCCACCTTCTTTTAGTCTTCTTCCTCCTCAATCAACTTCACTGAACATGAGCTTAGTTCTCATCAGTGGAAAATTCTCCAAAGTTGATTCTTTGGGGTCTCTTTGGTTGAATTCTTCTACTGATGTGCGAAAGAGTCCAATGGTTCGATTCAACTATTTTTCGCATCCCCGTGACCTTGCACAATGTATTGGAGGATTAAGAAAGATCCAAGATTTGTTGAACACACAAACGATAGAAAATATTAAGACAAAAGATTTGGAGGGCAAGAAAACACTTCAGTTTCTAGGGATTCCATTGCCGGAAAATATGGCAGATGATACTTTGGTTGGAGAGTTTTGTAAGAGAACGGTGACGACGTTCTGGCATTTCCATGGAGGATGCGTGGTCGGAAAAGTTGTTGATGGTACTTATAGAGTAATGGGAATCGAAAATCTTCGTGTAGTTGATGGCTCCACTTTCTCCGAGTCGCCAGGAACTAATCCTATGGCCACTATCATGATGCTCGGACGGTAAGTCTTTTTTTATATATATCTTTCAACTACCTGTTTTCACCCGTTTTGTTTGTAATAGAAATTTATAGAGAAGACAAACTTTAAAAACAAAAATTATTTCTCTTAGTTTTTTATTTTTGATTTGGTTTTCAAAATGTAAAATGTAAGGAGTGAGTTTAAACCTAAATTTAGAGTAAGTTTAAAGACAGTTTTTATTACTAGATCACTAGCTACTGATATTATTGTTATATATTAATTCAATTGTATATATACATACGTATGTATACACACATGTATATATTAACATTGAAATTTATCTAGTAGAACTAAAGTTTTTTAACTTAATCTTCAAACATCAAAAACATCTTTACAACTTCAAGTGATTTACAAACTTTACAATTTAAAAACCATGATCAATCCAACCCTGCAGATAATCGTGAGGTGAGAGATTCAATCTGTATATTAAAAAAATCAAAACTTCGTACGTTGTTACTATGAAAGTATTTTAGTATATTTATTTCTATAAAATAATGATATCGAAATGATTTTGCAGATATGTTGGCATGAAAATGCTTCAAGAAAGATTGAGTTTAGCGTAA

mRNA sequence

ATGGAGCACTCAACCATGTCTGCCTTTCTTCTTCACATCCTTATTTTCTGCTGTCAATCTCATTTAGGAGTCTTCTCCACCAGTTACACTAAACTCGGTAATAGTGATCAAGATGTCAGCTACATGAAGTTTGTGCACAATGCAAATAAGTTCCCAGAAAAAGAAGAATATGACTACATAATAATAGGAGGAGGAACTGCAGGCTGTCCATTAGCTGCAACATTATCATCAAAATTCTCCGTCCTCCTCTTAGAAAGAGGCAATATTCCCAACAAGTTTCCTTCTGTATTGAACAAACAAGGATTGATGAATGCTTTCACTGACAAAGACGATGGTGAAAATCCCTTCCAACGGTTCGTTTCCGAGGATGGCGTAGAGAATCTAAGAGGGCGGATTTTGGGTGGTAGTAGCATGATCAATGCTGGATTCTATTCAAGGGCTCATAAAGAGTTTTTTGAAACACAAGAAATAATTGAATGGGACATGGAAATGGTGAGAGAAGCTTATGAATGGGTTGAAGAGACTTTGGTTTCTGAACCAAATTTGAGTTCTTGGCAATTTGCTTTTAGAAAGGCATTGTTGGAAGTTGGGGTTGACCATGATAATGGGTTTGAGTTGAGACATCTTGTAGGGACTAAAATTGGAGGTTCTATCTTTGATAACCAAGGCAATAGACATGGAGCTGTAGAGCTTCTTAATAAGGGAGAATCTGAAAATCTTAAAGTTGCAGTTCAGGCTACAGTCAAAAGAATTCTCTTCTCTGGTTTATCTGCAAATGGGGTTTTGTATTCAGATTCGAAAGGAAAGTCACACACAGCATTCATCCATGAGAAAGGAGAGATAATTCTAAGTGCTGGAGCCATTGGTAGCCCTCAACTTCTCCTTCTAAGTGGGGTTGGCTCAACATCTCATCTTTCTTCTTTAAACCTTCCTCTCTTCCTTCACCAACCCCATGTCGGACAATTCATGTCGGACAACCCTCGATTTGGCGTCAATATTGTGCTTCCATTTCCTCTACCAACGACGACGGTGGAAGTCGTCGGAATTTTAGAAAAGAATACATATTTTGAGTCTTTGTCAAGCTTTATACCATTCTCAATTCCACCTTCTTTTAGTCTTCTTCCTCCTCAATCAACTTCACTGAACATGAGCTTAGTTCTCATCAGTGGAAAATTCTCCAAAGTTGATTCTTTGGGGTCTCTTTGGTTGAATTCTTCTACTGATGTGCGAAAGAGTCCAATGGTTCGATTCAACTATTTTTCGCATCCCCGTGACCTTGCACAATGTATTGGAGGATTAAGAAAGATCCAAGATTTGTTGAACACACAAACGATAGAAAATATTAAGACAAAAGATTTGGAGGGCAAGAAAACACTTCAGTTTCTAGGGATTCCATTGCCGGAAAATATGGCAGATGATACTTTGGTTGGAGAGTTTTGTAAGAGAACGGTGACGACGTTCTGGCATTTCCATGGAGGATGCGTGGTCGGAAAAGTTGTTGATGGTACTTATAGAGTAATGGGAATCGAAAATCTTCGTGTAGTTGATGGCTCCACTTTCTCCGAGTCGCCAGGAACTAATCCTATGGCCACTATCATGATGCTCGGACGATATGTTGGCATGAAAATGCTTCAAGAAAGATTGAGTTTAGCGTAA

Coding sequence (CDS)

ATGGAGCACTCAACCATGTCTGCCTTTCTTCTTCACATCCTTATTTTCTGCTGTCAATCTCATTTAGGAGTCTTCTCCACCAGTTACACTAAACTCGGTAATAGTGATCAAGATGTCAGCTACATGAAGTTTGTGCACAATGCAAATAAGTTCCCAGAAAAAGAAGAATATGACTACATAATAATAGGAGGAGGAACTGCAGGCTGTCCATTAGCTGCAACATTATCATCAAAATTCTCCGTCCTCCTCTTAGAAAGAGGCAATATTCCCAACAAGTTTCCTTCTGTATTGAACAAACAAGGATTGATGAATGCTTTCACTGACAAAGACGATGGTGAAAATCCCTTCCAACGGTTCGTTTCCGAGGATGGCGTAGAGAATCTAAGAGGGCGGATTTTGGGTGGTAGTAGCATGATCAATGCTGGATTCTATTCAAGGGCTCATAAAGAGTTTTTTGAAACACAAGAAATAATTGAATGGGACATGGAAATGGTGAGAGAAGCTTATGAATGGGTTGAAGAGACTTTGGTTTCTGAACCAAATTTGAGTTCTTGGCAATTTGCTTTTAGAAAGGCATTGTTGGAAGTTGGGGTTGACCATGATAATGGGTTTGAGTTGAGACATCTTGTAGGGACTAAAATTGGAGGTTCTATCTTTGATAACCAAGGCAATAGACATGGAGCTGTAGAGCTTCTTAATAAGGGAGAATCTGAAAATCTTAAAGTTGCAGTTCAGGCTACAGTCAAAAGAATTCTCTTCTCTGGTTTATCTGCAAATGGGGTTTTGTATTCAGATTCGAAAGGAAAGTCACACACAGCATTCATCCATGAGAAAGGAGAGATAATTCTAAGTGCTGGAGCCATTGGTAGCCCTCAACTTCTCCTTCTAAGTGGGGTTGGCTCAACATCTCATCTTTCTTCTTTAAACCTTCCTCTCTTCCTTCACCAACCCCATGTCGGACAATTCATGTCGGACAACCCTCGATTTGGCGTCAATATTGTGCTTCCATTTCCTCTACCAACGACGACGGTGGAAGTCGTCGGAATTTTAGAAAAGAATACATATTTTGAGTCTTTGTCAAGCTTTATACCATTCTCAATTCCACCTTCTTTTAGTCTTCTTCCTCCTCAATCAACTTCACTGAACATGAGCTTAGTTCTCATCAGTGGAAAATTCTCCAAAGTTGATTCTTTGGGGTCTCTTTGGTTGAATTCTTCTACTGATGTGCGAAAGAGTCCAATGGTTCGATTCAACTATTTTTCGCATCCCCGTGACCTTGCACAATGTATTGGAGGATTAAGAAAGATCCAAGATTTGTTGAACACACAAACGATAGAAAATATTAAGACAAAAGATTTGGAGGGCAAGAAAACACTTCAGTTTCTAGGGATTCCATTGCCGGAAAATATGGCAGATGATACTTTGGTTGGAGAGTTTTGTAAGAGAACGGTGACGACGTTCTGGCATTTCCATGGAGGATGCGTGGTCGGAAAAGTTGTTGATGGTACTTATAGAGTAATGGGAATCGAAAATCTTCGTGTAGTTGATGGCTCCACTTTCTCCGAGTCGCCAGGAACTAATCCTATGGCCACTATCATGATGCTCGGACGATATGTTGGCATGAAAATGCTTCAAGAAAGATTGAGTTTAGCGTAA

Protein sequence

MEHSTMSAFLLHILIFCCQSHLGVFSTSYTKLGNSDQDVSYMKFVHNANKFPEKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQGLMNAFTDKDDGENPFQRFVSEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWDMEMVREAYEWVEETLVSEPNLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDNQGNRHGAVELLNKGESENLKVAVQATVKRILFSGLSANGVLYSDSKGKSHTAFIHEKGEIILSAGAIGSPQLLLLSGVGSTSHLSSLNLPLFLHQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLSSFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLGSLWLNSSTDVRKSPMVRFNYFSHPRDLAQCIGGLRKIQDLLNTQTIENIKTKDLEGKKTLQFLGIPLPENMADDTLVGEFCKRTVTTFWHFHGGCVVGKVVDGTYRVMGIENLRVVDGSTFSESPGTNPMATIMMLGRYVGMKMLQERLSLA*
Homology
BLAST of CsGy2G005902 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 2.3e-155
Identity = 277/553 (50.09%), Postives = 381/553 (68.90%), Query Frame = 0

Query: 1   MEHSTMSAFL--LHILIFCCQSHLGVFSTSYTKLGNSDQDVSYMKFVHNANKFPEKEEYD 60
           ME STMSA L  LH+ +   Q     +S  ++    S+ D SY++F ++A     +  YD
Sbjct: 1   MEKSTMSAILLVLHLFVLLLQ-----YSEVHSLATTSNHDFSYLRFAYDATDLELEGSYD 60

Query: 61  YIIIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQGLMNAFTDKDDGENPFQR 120
           Y+I+GGGT+GCPLAATLS K+ VL+LERG++P  +P+VL   G +     +DDG+ P +R
Sbjct: 61  YVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVER 120

Query: 121 FVSEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWDMEMVREAYEWVEETLVS 180
           FVSEDG++N+RGR+LGG+SMINAG Y+RA+   +     ++WDM++V + YEWVE+T+V 
Sbjct: 121 FVSEDGIDNVRGRVLGGTSMINAGVYARANTSIYSASG-VDWDMDLVNKTYEWVEDTIVF 180

Query: 181 EPNLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDNQGNRHGAVELLNKGESE 240
           +PN   WQ     A LE GVD ++GF L H  GT+I GS FDN+G RH A ELLNKG S 
Sbjct: 181 KPNYQPWQSVTGTAFLEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSN 240

Query: 241 NLKVAVQATVKRILFS---GLSANGVLYSDSKGKSHTAFIHEKGEIILSAGAIGSPQLLL 300
           NL+V V A+V++I+FS   GL+A GV+Y DS G  H AF+  KGE+I+SAG IG+PQLLL
Sbjct: 241 NLRVGVHASVEKIIFSNAPGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLL 300

Query: 301 LSGVGSTSHLSSLNLPLFLHQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTY 360
           LSGVG  S+LSSLN+P+ L  P+VGQF+ DNPR  +NI+ P P+  T V V+GI   N +
Sbjct: 301 LSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI--SNDF 360

Query: 361 FESLSSFIPFSIPPSFSLLPPQSTSL-NMSLVLISGKFSKVDSLGSLWLNSSTDVRKSPM 420
           ++   S +PF+ PP FS  P  S  L N +    + K +   S GSL L SS++VR SP 
Sbjct: 361 YQCSFSSLPFTTPP-FSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPN 420

Query: 421 VRFNYFSHPRDLAQCIGGLRKIQDLLNTQTIENIKTKDLEGKKTLQFLGIPLPENMADDT 480
           V+FNY+S+P DL+ C+ G++KI +LL+T  ++  K +DL G +    LGIPLP++  DD 
Sbjct: 421 VKFNYYSNPTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDA 480

Query: 481 LVGEFCKRTVTTFWHFHGGCVVGKVVDGTYRVMGIENLRVVDGSTFSESPGTNPMATIMM 540
               FC+ +V ++WH+HGGC+VGKV+DG +RV GI+ LRVVDGSTF  +P ++P    +M
Sbjct: 481 AFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLM 540

Query: 541 LGRYVGMKMLQER 548
           LGRYVG+K+LQER
Sbjct: 541 LGRYVGIKILQER 544

BLAST of CsGy2G005902 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 5.6e-154
Identity = 277/551 (50.27%), Postives = 379/551 (68.78%), Query Frame = 0

Query: 1   MEHSTMSAFLLHILIFCCQSHLGVFSTSYTKLGNSDQDVSYMKFVHNANKFPEKEEYDYI 60
           ME STMSA LL + IF    HL  +S  ++    SD D SY+ F ++A     +  YDY+
Sbjct: 1   MEKSTMSAILLVLYIFVL--HL-QYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYV 60

Query: 61  IIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQGLMNAFTDKDDGENPFQRFV 120
           I+GGGT+GCPLAATLS K+ VL+LERG++P  +P+VL   G +     +DDG+ P +RFV
Sbjct: 61  IVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFV 120

Query: 121 SEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWDMEMVREAYEWVEETLVSEP 180
           SEDG++N+RGR+LGG+S+INAG Y+RA+   +     ++WDM++V + YEWVE+T+V +P
Sbjct: 121 SEDGIDNVRGRVLGGTSIINAGVYARANTSIYSASG-VDWDMDLVNQTYEWVEDTIVYKP 180

Query: 181 NLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDNQGNRHGAVELLNKGESENL 240
           N  SWQ   + A LE GV  ++GF L H  GT+I GS FDN+G RH A ELLNKG S NL
Sbjct: 181 NSQSWQSVTKTAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNL 240

Query: 241 KVAVQATVKRILFS---GLSANGVLYSDSKGKSHTAFIHEKGEIILSAGAIGSPQLLLLS 300
           +V V A+V++I+FS   GL+A GV+Y DS G  H AF+  KGE+I+SAG IG+PQLLLLS
Sbjct: 241 RVGVHASVEKIIFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLS 300

Query: 301 GVGSTSHLSSLNLPLFLHQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFE 360
           GVG  S+LSSLN+P+ L  P+VGQF+ DNPR  +NI+ P P+  T V V+GI   N +++
Sbjct: 301 GVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI--SNDFYQ 360

Query: 361 SLSSFIPFSIPPSFSLLPPQSTSL-NMSLVLISGKFSKVDSLGSLWLNSSTDVRKSPMVR 420
              S +PF+ PP F   P  S  L N +    + K +   S GSL L SS++VR SP V+
Sbjct: 361 CSFSSLPFTTPP-FGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVK 420

Query: 421 FNYFSHPRDLAQCIGGLRKIQDLLNTQTIENIKTKDLEGKKTLQFLGIPLPENMADDTLV 480
           FNY+S+  DL+ C+ G++KI +LL+T  ++  K +DL G +    LGIPLP++  DD   
Sbjct: 421 FNYYSNLTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAF 480

Query: 481 GEFCKRTVTTFWHFHGGCVVGKVVDGTYRVMGIENLRVVDGSTFSESPGTNPMATIMMLG 540
             FC+ +V ++WH+HGGC+VGKV+DG +RV GI  LRVVDGSTF  +P ++P    +MLG
Sbjct: 481 ETFCRESVASYWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLG 540

Query: 541 RYVGMKMLQER 548
           RYVG+K+LQER
Sbjct: 541 RYVGIKILQER 544

BLAST of CsGy2G005902 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 4.4e-151
Identity = 262/516 (50.78%), Postives = 362/516 (70.16%), Query Frame = 0

Query: 41  YMKFVHNANKFPEKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQ 100
           +M+F+ NA  F  ++ YDYII+GGGTAGCPLAATLS  F VLLLERG +P   P+V++  
Sbjct: 38  FMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSHD 97

Query: 101 GLMNAFTDKDDGENPFQRFVSEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEW 160
           G +   TD ++ ++P Q F+SE+GV N RGR+LGGSS INAGFYSRA K+FFE   ++ W
Sbjct: 98  GFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGLV-W 157

Query: 161 DMEMVREAYEWVEETLVSEPNLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFD 220
           D+  V ++YEWVE  +V  P L +WQ A R ALLEVGV   NGF L H VGTKIGGS FD
Sbjct: 158 DLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFD 217

Query: 221 NQGNRHGAVELLNKGESENLKVAVQATVKRILF--------SGLSANGVLYSDSKGKSHT 280
             G RH + +LL    S N++VAV ATV+R+L         S +SA GV+Y D  G+ H 
Sbjct: 218 RTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHH 277

Query: 281 AFIHEKGEIILSAGAIGSPQLLLLSGVGSTSHLSSLNLPLFLHQPHVGQFMSDNPRFGVN 340
           A I ++GE+ILSAGA+GSPQLL LSG+G  S+LS+  +P+ L QPHVG F+ DNPR G++
Sbjct: 278 ALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGIS 337

Query: 341 IVLPFPLPTTTVEVVGILEKNTYFESLSSFIPFSIPPSFSLLPPQSTSLNMSLVLISGKF 400
           IV P P+  + ++VVG+ E   + E+ S+ IPF+ P     +   ++ L + +  I  K 
Sbjct: 338 IVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVPVTTIMEKI 397

Query: 401 SKVDSLGSLWLNSSTDVRKSPMVRFNYFSHPRDLAQCIGGLRKIQDLLNTQTIENIKTKD 460
               S+G L L +STDVR +P+VRFNYFS P+DL +C+ G RKI ++L ++ +++   ++
Sbjct: 398 LGPVSIGLLRL-ASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIRE 457

Query: 461 LEGKKTLQFLGIPLPENMADDTLVGEFCKRTVTTFWHFHGGCVVGKVVDGTYRVMGIENL 520
             G +  +F+G PLP + ++D ++ +FC+RTV+T WH+HGG VVGKVVD   +V+G+ +L
Sbjct: 458 WFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSL 517

Query: 521 RVVDGSTFSESPGTNPMATIMMLGRYVGMKMLQERL 549
           R+VDGSTF+ SPGTNP AT+MMLGRY+G+KML+ER+
Sbjct: 518 RLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRERM 551

BLAST of CsGy2G005902 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 6.4e-150
Identity = 275/552 (49.82%), Postives = 378/552 (68.48%), Query Frame = 0

Query: 1   MEHSTMSAFLLHILIFCCQSHLGVFSTSYTKLGNSDQDVSYMKFVHNANKFPEKEEYDYI 60
           M  STMSA LL + IF    HL  +S   +    S  D SY+ FV++A     +  YDYI
Sbjct: 1   MVKSTMSAVLLVLHIFVL--HL-QYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYI 60

Query: 61  IIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQGLMNAFTDKDDGENPFQRFV 120
           I+GGGTAGCPLAATLS+ +SVL+LERG++P ++P++L   G +     +DDG+ P +RFV
Sbjct: 61  IVGGGTAGCPLAATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFV 120

Query: 121 SEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWDMEMVREAYEWVEETLVSEP 180
           SEDG++N+RGR+LGG+SMINAG Y RA+  FF  Q  IEWDM++V + YEWVE+T+V EP
Sbjct: 121 SEDGIDNVRGRVLGGTSMINAGVYVRANTSFF-NQTGIEWDMDLVNQTYEWVEDTIVFEP 180

Query: 181 NLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDNQGNRHGAVELLNKGESENL 240
           +  +WQ     A LE G+  +NGF + HL GT++ GS FDN G RH + ELLNKG+  NL
Sbjct: 181 DSQTWQTVIGTAYLEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNL 240

Query: 241 KVAVQATVKRILF----SGLSANGVLYSDSKGKSHTAFIHEKGEIILSAGAIGSPQLLLL 300
           +VAVQA V++I+F    SG++A GV+Y+DS G +H AF+  +GE+ILSAG IGSPQLLLL
Sbjct: 241 RVAVQAAVEKIIFSSNTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLL 300

Query: 301 SGVGSTSHLSSLNLPLFLHQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYF 360
           SGVG  S+L+SLN+ +    P+VGQ++ DNPR  +NI+ P P+  +TV V+GI   + ++
Sbjct: 301 SGVGPESYLTSLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGI--TSDFY 360

Query: 361 ESLSSFIPFSIPPSFSLLPPQSTSL-NMSLVLISGKFSKVDSLGSLWLNSSTDVRKSPMV 420
           +   S +PF  PP FS  P  S  L N +   I  K     S G++ LNSS+DVR  P V
Sbjct: 361 QCSISSLPFDTPP-FSFFPTTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNV 420

Query: 421 RFNYFSHPRDLAQCIGGLRKIQDLLNTQTIENIKTKDLEGKKTLQFLGIPLPENMADDTL 480
           +FNY+S+  DL+ C+ G++K+ ++L+T  +E  K +DL G      LGIPLPEN  DD  
Sbjct: 421 KFNYYSNLTDLSHCVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAA 480

Query: 481 VGEFCKRTVTTFWHFHGGCVVGKVVDGTYRVMGIENLRVVDGSTFSESPGTNPMATIMML 540
              FC+ +V ++WH+HGGC+VGKV+D  +RV GI  LRVVDGSTF  +P ++P    +ML
Sbjct: 481 FETFCRESVASYWHYHGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLML 540

Query: 541 GRYVGMKMLQER 548
           GRY+G+++LQER
Sbjct: 541 GRYMGIQILQER 545

BLAST of CsGy2G005902 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 2.0e-148
Identity = 276/554 (49.82%), Postives = 373/554 (67.33%), Query Frame = 0

Query: 1   MEHSTMSA--FLLHILIFCCQSHLGVFSTSYTKLGNSDQDVSYMKFVHNANKFPEKEEYD 60
           ME STMS   F+LH+L+   Q     +S  ++    S  D SY+KFV+NA     +  YD
Sbjct: 1   MEKSTMSVILFVLHLLVLHLQ-----YSEVHSLANTSAHDFSYLKFVYNATDTSLEGSYD 60

Query: 61  YIIIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQGLMNAFTDKDDGENPFQR 120
           YI+IGGGT+GCPLAATLS K+ VLLLERG I  ++P+ L   G       +DDG+ P +R
Sbjct: 61  YIVIGGGTSGCPLAATLSEKYKVLLLERGTIATEYPNTLTADGFAYNLQQQDDGKTPVER 120

Query: 121 FVSEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWDMEMVREAYEWVEETLVS 180
           FVSEDG++N+R RILGG+++INAG Y+RA+  F+ +Q  IEWD+++V + YEWVE+ +V 
Sbjct: 121 FVSEDGIDNVRARILGGTTIINAGVYARANISFY-SQTGIEWDLDLVNKTYEWVEDAIVV 180

Query: 181 EPNLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDNQGNRHGAVELLNKGESE 240
           +PN  SWQ    +  LE G+  DNGF L H  GT++ GS FDN G RH A ELLNKG+  
Sbjct: 181 KPNNQSWQSVIGEGFLEAGILPDNGFSLDHEAGTRLTGSTFDNNGTRHAADELLNKGDPN 240

Query: 241 NLKVAVQATVKRILF----SGLSANGVLYSDSKGKSHTAFIHEKGEIILSAGAIGSPQLL 300
           NL VAVQA+V++ILF    S LSA GV+Y+DS G SH AF+   GE+I+SAG IG+PQLL
Sbjct: 241 NLLVAVQASVEKILFSSNTSNLSAIGVIYTDSDGNSHQAFVRGNGEVIVSAGTIGTPQLL 300

Query: 301 LLSGVGSTSHLSSLNLPLFLHQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNT 360
           LLSGVG  S+LSSLN+ +    P+VGQF+ +NPR  +N   P P+  + V V+GI  ++ 
Sbjct: 301 LLSGVGPESYLSSLNITVVQPNPYVGQFLYNNPRNFINNFPPNPIEASVVTVLGI--RSD 360

Query: 361 YFESLSSFIPFSIPPSFSLLPPQSTSL-NMSLVLISGKFSKVDSLGSLWLNSSTDVRKSP 420
           Y++   S +PFS PP FSL P  S  L N +   I  +     S GS+ LNSS+DVR +P
Sbjct: 361 YYQVSLSSLPFSTPP-FSLFPTTSYPLPNSTFAHIVSQVPGPLSHGSVTLNSSSDVRIAP 420

Query: 421 MVRFNYFSHPRDLAQCIGGLRKIQDLLNTQTIENIKTKDLEGKKTLQFLGIPLPENMADD 480
            ++FNY+S+  DLA C+ G++K+ DLL T+ +E  K +D+ G     +LG+PLPEN  DD
Sbjct: 421 NIKFNYYSNSTDLANCVSGMKKLGDLLRTKALEPYKARDVLGIDGFNYLGVPLPENQTDD 480

Query: 481 TLVGEFCKRTVTTFWHFHGGCVVGKVVDGTYRVMGIENLRVVDGSTFSESPGTNPMATIM 540
                FC   V ++WH+HGG +VGKV+D ++RVMGI+ LRVVD STF   P ++P    +
Sbjct: 481 ASFETFCLDNVASYWHYHGGSLVGKVLDDSFRVMGIKALRVVDASTFPYEPNSHPQGFYL 540

Query: 541 MLGRYVGMKMLQER 548
           MLGRYVG+++LQER
Sbjct: 541 MLGRYVGLQILQER 545

BLAST of CsGy2G005902 vs. NCBI nr
Match: KAE8651657.1 (hypothetical protein Csa_021352 [Cucumis sativus])

HSP 1 Score: 1103 bits (2853), Expect = 0.0
Identity = 551/551 (100.00%), Postives = 551/551 (100.00%), Query Frame = 0

Query: 1   MEHSTMSAFLLHILIFCCQSHLGVFSTSYTKLGNSDQDVSYMKFVHNANKFPEKEEYDYI 60
           MEHSTMSAFLLHILIFCCQSHLGVFSTSYTKLGNSDQDVSYMKFVHNANKFPEKEEYDYI
Sbjct: 1   MEHSTMSAFLLHILIFCCQSHLGVFSTSYTKLGNSDQDVSYMKFVHNANKFPEKEEYDYI 60

Query: 61  IIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQGLMNAFTDKDDGENPFQRFV 120
           IIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQGLMNAFTDKDDGENPFQRFV
Sbjct: 61  IIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQGLMNAFTDKDDGENPFQRFV 120

Query: 121 SEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWDMEMVREAYEWVEETLVSEP 180
           SEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWDMEMVREAYEWVEETLVSEP
Sbjct: 121 SEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWDMEMVREAYEWVEETLVSEP 180

Query: 181 NLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDNQGNRHGAVELLNKGESENL 240
           NLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDNQGNRHGAVELLNKGESENL
Sbjct: 181 NLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDNQGNRHGAVELLNKGESENL 240

Query: 241 KVAVQATVKRILFSGLSANGVLYSDSKGKSHTAFIHEKGEIILSAGAIGSPQLLLLSGVG 300
           KVAVQATVKRILFSGLSANGVLYSDSKGKSHTAFIHEKGEIILSAGAIGSPQLLLLSGVG
Sbjct: 241 KVAVQATVKRILFSGLSANGVLYSDSKGKSHTAFIHEKGEIILSAGAIGSPQLLLLSGVG 300

Query: 301 STSHLSSLNLPLFLHQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLS 360
           STSHLSSLNLPLFLHQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLS
Sbjct: 301 STSHLSSLNLPLFLHQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLS 360

Query: 361 SFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLGSLWLNSSTDVRKSPMVRFNYF 420
           SFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLGSLWLNSSTDVRKSPMVRFNYF
Sbjct: 361 SFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLGSLWLNSSTDVRKSPMVRFNYF 420

Query: 421 SHPRDLAQCIGGLRKIQDLLNTQTIENIKTKDLEGKKTLQFLGIPLPENMADDTLVGEFC 480
           SHPRDLAQCIGGLRKIQDLLNTQTIENIKTKDLEGKKTLQFLGIPLPENMADDTLVGEFC
Sbjct: 421 SHPRDLAQCIGGLRKIQDLLNTQTIENIKTKDLEGKKTLQFLGIPLPENMADDTLVGEFC 480

Query: 481 KRTVTTFWHFHGGCVVGKVVDGTYRVMGIENLRVVDGSTFSESPGTNPMATIMMLGRYVG 540
           KRTVTTFWHFHGGCVVGKVVDGTYRVMGIENLRVVDGSTFSESPGTNPMATIMMLGRYVG
Sbjct: 481 KRTVTTFWHFHGGCVVGKVVDGTYRVMGIENLRVVDGSTFSESPGTNPMATIMMLGRYVG 540

Query: 541 MKMLQERLSLA 551
           MKMLQERLSLA
Sbjct: 541 MKMLQERLSLA 551

BLAST of CsGy2G005902 vs. NCBI nr
Match: XP_008452076.1 (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo])

HSP 1 Score: 1044 bits (2700), Expect = 0.0
Identity = 518/551 (94.01%), Postives = 537/551 (97.46%), Query Frame = 0

Query: 1   MEHSTMSAFLLHILIFCCQSHLGVFSTSYTKLGNSDQDVSYMKFVHNANKFPEKEEYDYI 60
           MEHSTMSAFLLHILIF  QSHLGVFST+YTK  NS+QDVSY+KFVHNANK PEKEEYDYI
Sbjct: 1   MEHSTMSAFLLHILIFFFQSHLGVFSTTYTKPSNSNQDVSYLKFVHNANKLPEKEEYDYI 60

Query: 61  IIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQGLMNAFTDKDDGENPFQRFV 120
           IIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSV+NKQGLMNAFTDKD+GENPFQRFV
Sbjct: 61  IIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVMNKQGLMNAFTDKDNGENPFQRFV 120

Query: 121 SEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWDMEMVREAYEWVEETLVSEP 180
           SEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEI EWDMEMV +AYEWVEETLVS+P
Sbjct: 121 SEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEISEWDMEMVGKAYEWVEETLVSKP 180

Query: 181 NLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDNQGNRHGAVELLNKGESENL 240
           NLSSWQFAFRKALLEVG+DHDNGF+LRH++GTKIGGSIFDNQGNRHGAVELLNKGE ENL
Sbjct: 181 NLSSWQFAFRKALLEVGIDHDNGFDLRHVLGTKIGGSIFDNQGNRHGAVELLNKGEFENL 240

Query: 241 KVAVQATVKRILFSGLSANGVLYSDSKGKSHTAFIHEKGEIILSAGAIGSPQLLLLSGVG 300
           KVAVQATVKRILFSGLSANGVLYSDSKGK+HTAFIHEKGEIILSAGAIGSPQLLLLSG+G
Sbjct: 241 KVAVQATVKRILFSGLSANGVLYSDSKGKTHTAFIHEKGEIILSAGAIGSPQLLLLSGIG 300

Query: 301 STSHLSSLNLPLFLHQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLS 360
           STSHLSSL+LPL  HQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLS
Sbjct: 301 STSHLSSLDLPLVFHQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLS 360

Query: 361 SFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLGSLWLNSSTDVRKSPMVRFNYF 420
           SFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLG LWLNSSTDV+KSPMVRFNYF
Sbjct: 361 SFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLGFLWLNSSTDVQKSPMVRFNYF 420

Query: 421 SHPRDLAQCIGGLRKIQDLLNTQTIENIKTKDLEGKKTLQFLGIPLPENMADDTLVGEFC 480
           SHP DLAQCIGGLRKIQDLLNTQTIENIKT+DLEGKKTLQF+G+PLPENMADDTLVGEFC
Sbjct: 421 SHPHDLAQCIGGLRKIQDLLNTQTIENIKTQDLEGKKTLQFIGLPLPENMADDTLVGEFC 480

Query: 481 KRTVTTFWHFHGGCVVGKVVDGTYRVMGIENLRVVDGSTFSESPGTNPMATIMMLGRYVG 540
           KRTVTTFWHFHGGCVVGKVVDG YRVMGIENLRVVDGSTF ESPGTNPMATIMMLGRYVG
Sbjct: 481 KRTVTTFWHFHGGCVVGKVVDGNYRVMGIENLRVVDGSTFFESPGTNPMATIMMLGRYVG 540

Query: 541 MKMLQERLSLA 551
           MKMLQERL+LA
Sbjct: 541 MKMLQERLNLA 551

BLAST of CsGy2G005902 vs. NCBI nr
Match: KAA0067271.1 ((R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1020 bits (2638), Expect = 0.0
Identity = 505/537 (94.04%), Postives = 523/537 (97.39%), Query Frame = 0

Query: 1   MEHSTMSAFLLHILIFCCQSHLGVFSTSYTKLGNSDQDVSYMKFVHNANKFPEKEEYDYI 60
           MEHSTMSAFLLHILIF  QSHLGVFST+YTK  NS+QDVSY+KFVHNANK PEKEEYDYI
Sbjct: 1   MEHSTMSAFLLHILIFFFQSHLGVFSTTYTKPSNSNQDVSYLKFVHNANKLPEKEEYDYI 60

Query: 61  IIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQGLMNAFTDKDDGENPFQRFV 120
           IIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSV+NKQGLMNAFTDKD+GENPFQRFV
Sbjct: 61  IIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVMNKQGLMNAFTDKDNGENPFQRFV 120

Query: 121 SEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWDMEMVREAYEWVEETLVSEP 180
           SEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEI EWDMEMV +AYEWVEETLVS+P
Sbjct: 121 SEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEISEWDMEMVGKAYEWVEETLVSKP 180

Query: 181 NLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDNQGNRHGAVELLNKGESENL 240
           NLSSWQFAFRKALLEVG+DHDNGF+LRH++GTKIGGSIFDNQGNRHGAVELLNKGE ENL
Sbjct: 181 NLSSWQFAFRKALLEVGIDHDNGFDLRHVLGTKIGGSIFDNQGNRHGAVELLNKGEFENL 240

Query: 241 KVAVQATVKRILFSGLSANGVLYSDSKGKSHTAFIHEKGEIILSAGAIGSPQLLLLSGVG 300
           KVAVQATVKRILFSGLSANGVLYSDSKGK+HTAFIHEKGEIILSAGAIGSPQLLLLSG+G
Sbjct: 241 KVAVQATVKRILFSGLSANGVLYSDSKGKTHTAFIHEKGEIILSAGAIGSPQLLLLSGIG 300

Query: 301 STSHLSSLNLPLFLHQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLS 360
           STSHLSSL+LPL  HQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLS
Sbjct: 301 STSHLSSLDLPLVFHQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLS 360

Query: 361 SFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLGSLWLNSSTDVRKSPMVRFNYF 420
           SFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLG LWLNSSTDV+KSPMVRFNYF
Sbjct: 361 SFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLGFLWLNSSTDVQKSPMVRFNYF 420

Query: 421 SHPRDLAQCIGGLRKIQDLLNTQTIENIKTKDLEGKKTLQFLGIPLPENMADDTLVGEFC 480
           SHP DLAQCIGGLRKIQDLLNTQTIENIKT+DLEGKKTLQF+G+PLPENMADDTLVGEFC
Sbjct: 421 SHPHDLAQCIGGLRKIQDLLNTQTIENIKTQDLEGKKTLQFIGLPLPENMADDTLVGEFC 480

Query: 481 KRTVTTFWHFHGGCVVGKVVDGTYRVMGIENLRVVDGSTFSESPGTNPMATIMMLGR 537
           KRTVTTFWHFHGGCVVGKVVDG YRVMGIENLRVVDGSTF ESPGTNPMATIMMLGR
Sbjct: 481 KRTVTTFWHFHGGCVVGKVVDGNYRVMGIENLRVVDGSTFFESPGTNPMATIMMLGR 537

BLAST of CsGy2G005902 vs. NCBI nr
Match: XP_031736690.1 ((R)-mandelonitrile lyase-like [Cucumis sativus])

HSP 1 Score: 1011 bits (2615), Expect = 0.0
Identity = 510/528 (96.59%), Postives = 510/528 (96.59%), Query Frame = 0

Query: 42  MKFVHNANKFPEKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQG 101
           MKFVHNANKFPEKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQG
Sbjct: 1   MKFVHNANKFPEKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQG 60

Query: 102 LMNAFTDKDDGENPFQRFVSEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWD 161
           LMNAFTDKDDGENPFQRFVSEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWD
Sbjct: 61  LMNAFTDKDDGENPFQRFVSEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWD 120

Query: 162 MEMVREAYEWVEETLVSEPNLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDN 221
           MEMVREAYEWVEETLVSEPNLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDN
Sbjct: 121 MEMVREAYEWVEETLVSEPNLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDN 180

Query: 222 QGNRHGAVELLNKGESENLKVAVQATVKRILFS------------------GLSANGVLY 281
           QGNRHGAVELLNKGESENLKVAVQATVKRILFS                  GLSANGVLY
Sbjct: 181 QGNRHGAVELLNKGESENLKVAVQATVKRILFSDVCVNLLNSLHTIYMNFVGLSANGVLY 240

Query: 282 SDSKGKSHTAFIHEKGEIILSAGAIGSPQLLLLSGVGSTSHLSSLNLPLFLHQPHVGQFM 341
           SDSKGKSHTAFIHEKGEIILSAGAIGSPQLLLLSGVGSTSHLSSLNLPLFLHQPHVGQFM
Sbjct: 241 SDSKGKSHTAFIHEKGEIILSAGAIGSPQLLLLSGVGSTSHLSSLNLPLFLHQPHVGQFM 300

Query: 342 SDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLSSFIPFSIPPSFSLLPPQSTSLNM 401
           SDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLSSFIPFSIPPSFSLLPPQSTSLNM
Sbjct: 301 SDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLSSFIPFSIPPSFSLLPPQSTSLNM 360

Query: 402 SLVLISGKFSKVDSLGSLWLNSSTDVRKSPMVRFNYFSHPRDLAQCIGGLRKIQDLLNTQ 461
           SLVLISGKFSKVDSLGSLWLNSSTDVRKSPMVRFNYFSHPRDLAQCIGGLRKIQDLLNTQ
Sbjct: 361 SLVLISGKFSKVDSLGSLWLNSSTDVRKSPMVRFNYFSHPRDLAQCIGGLRKIQDLLNTQ 420

Query: 462 TIENIKTKDLEGKKTLQFLGIPLPENMADDTLVGEFCKRTVTTFWHFHGGCVVGKVVDGT 521
           TIENIKTKDLEGKKTLQFLGIPLPENMADDTLVGEFCKRTVTTFWHFHGGCVVGKVVDGT
Sbjct: 421 TIENIKTKDLEGKKTLQFLGIPLPENMADDTLVGEFCKRTVTTFWHFHGGCVVGKVVDGT 480

Query: 522 YRVMGIENLRVVDGSTFSESPGTNPMATIMMLGRYVGMKMLQERLSLA 551
           YRVMGIENLRVVDGSTFSESPGTNPMATIMMLGRYVGMKMLQERLSLA
Sbjct: 481 YRVMGIENLRVVDGSTFSESPGTNPMATIMMLGRYVGMKMLQERLSLA 528

BLAST of CsGy2G005902 vs. NCBI nr
Match: TYK10883.1 ((R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 958 bits (2476), Expect = 0.0
Identity = 471/496 (94.96%), Postives = 486/496 (97.98%), Query Frame = 0

Query: 42  MKFVHNANKFPEKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQG 101
           MKFVHNANK PEKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSV+NKQG
Sbjct: 1   MKFVHNANKLPEKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVMNKQG 60

Query: 102 LMNAFTDKDDGENPFQRFVSEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWD 161
           LMNAFTDKD+GENPFQRFVSEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEI EWD
Sbjct: 61  LMNAFTDKDNGENPFQRFVSEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEISEWD 120

Query: 162 MEMVREAYEWVEETLVSEPNLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDN 221
           MEMV +AYEWVEETLVS+PNLSSWQFAFRKALLEVG+DHDNGF+LRH++GTKIGGSIFDN
Sbjct: 121 MEMVGKAYEWVEETLVSKPNLSSWQFAFRKALLEVGIDHDNGFDLRHVLGTKIGGSIFDN 180

Query: 222 QGNRHGAVELLNKGESENLKVAVQATVKRILFSGLSANGVLYSDSKGKSHTAFIHEKGEI 281
           QGNRHGAVELLNKGE ENLKVAVQATVKRILFSGLSANGVLYSDSKGK+HTAFIHEKGEI
Sbjct: 181 QGNRHGAVELLNKGEFENLKVAVQATVKRILFSGLSANGVLYSDSKGKTHTAFIHEKGEI 240

Query: 282 ILSAGAIGSPQLLLLSGVGSTSHLSSLNLPLFLHQPHVGQFMSDNPRFGVNIVLPFPLPT 341
           ILSAGAIGSPQLLLLSG+GSTSHLSSL+LPL  HQPHVGQFMSDNPRFGVNIVLPFPLPT
Sbjct: 241 ILSAGAIGSPQLLLLSGIGSTSHLSSLDLPLVFHQPHVGQFMSDNPRFGVNIVLPFPLPT 300

Query: 342 TTVEVVGILEKNTYFESLSSFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLGSL 401
           TTVEVVGILEKNTYFESLSSFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLG L
Sbjct: 301 TTVEVVGILEKNTYFESLSSFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLGCL 360

Query: 402 WLNSSTDVRKSPMVRFNYFSHPRDLAQCIGGLRKIQDLLNTQTIENIKTKDLEGKKTLQF 461
           WLNSSTDV+KSPMVRFNYFSHP DLAQCIGGLRKIQDLLNTQTIENIKT+DLEGKKTLQF
Sbjct: 361 WLNSSTDVQKSPMVRFNYFSHPHDLAQCIGGLRKIQDLLNTQTIENIKTQDLEGKKTLQF 420

Query: 462 LGIPLPENMADDTLVGEFCKRTVTTFWHFHGGCVVGKVVDGTYRVMGIENLRVVDGSTFS 521
           +G+PLPENMADDTLVGEFCKRTVTTFWHFHGGCVVGKVVDG YRVMGIENLRVVDGSTF 
Sbjct: 421 IGLPLPENMADDTLVGEFCKRTVTTFWHFHGGCVVGKVVDGNYRVMGIENLRVVDGSTFF 480

Query: 522 ESPGTNPMATIMMLGR 537
           ESPGTNPMATIMMLGR
Sbjct: 481 ESPGTNPMATIMMLGR 496

BLAST of CsGy2G005902 vs. ExPASy TrEMBL
Match: A0A0A0LMB3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G050000 PE=3 SV=1)

HSP 1 Score: 1093 bits (2826), Expect = 0.0
Identity = 546/546 (100.00%), Postives = 546/546 (100.00%), Query Frame = 0

Query: 6   MSAFLLHILIFCCQSHLGVFSTSYTKLGNSDQDVSYMKFVHNANKFPEKEEYDYIIIGGG 65
           MSAFLLHILIFCCQSHLGVFSTSYTKLGNSDQDVSYMKFVHNANKFPEKEEYDYIIIGGG
Sbjct: 1   MSAFLLHILIFCCQSHLGVFSTSYTKLGNSDQDVSYMKFVHNANKFPEKEEYDYIIIGGG 60

Query: 66  TAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQGLMNAFTDKDDGENPFQRFVSEDGV 125
           TAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQGLMNAFTDKDDGENPFQRFVSEDGV
Sbjct: 61  TAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQGLMNAFTDKDDGENPFQRFVSEDGV 120

Query: 126 ENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWDMEMVREAYEWVEETLVSEPNLSSW 185
           ENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWDMEMVREAYEWVEETLVSEPNLSSW
Sbjct: 121 ENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWDMEMVREAYEWVEETLVSEPNLSSW 180

Query: 186 QFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDNQGNRHGAVELLNKGESENLKVAVQ 245
           QFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDNQGNRHGAVELLNKGESENLKVAVQ
Sbjct: 181 QFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDNQGNRHGAVELLNKGESENLKVAVQ 240

Query: 246 ATVKRILFSGLSANGVLYSDSKGKSHTAFIHEKGEIILSAGAIGSPQLLLLSGVGSTSHL 305
           ATVKRILFSGLSANGVLYSDSKGKSHTAFIHEKGEIILSAGAIGSPQLLLLSGVGSTSHL
Sbjct: 241 ATVKRILFSGLSANGVLYSDSKGKSHTAFIHEKGEIILSAGAIGSPQLLLLSGVGSTSHL 300

Query: 306 SSLNLPLFLHQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLSSFIPF 365
           SSLNLPLFLHQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLSSFIPF
Sbjct: 301 SSLNLPLFLHQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLSSFIPF 360

Query: 366 SIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLGSLWLNSSTDVRKSPMVRFNYFSHPRD 425
           SIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLGSLWLNSSTDVRKSPMVRFNYFSHPRD
Sbjct: 361 SIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLGSLWLNSSTDVRKSPMVRFNYFSHPRD 420

Query: 426 LAQCIGGLRKIQDLLNTQTIENIKTKDLEGKKTLQFLGIPLPENMADDTLVGEFCKRTVT 485
           LAQCIGGLRKIQDLLNTQTIENIKTKDLEGKKTLQFLGIPLPENMADDTLVGEFCKRTVT
Sbjct: 421 LAQCIGGLRKIQDLLNTQTIENIKTKDLEGKKTLQFLGIPLPENMADDTLVGEFCKRTVT 480

Query: 486 TFWHFHGGCVVGKVVDGTYRVMGIENLRVVDGSTFSESPGTNPMATIMMLGRYVGMKMLQ 545
           TFWHFHGGCVVGKVVDGTYRVMGIENLRVVDGSTFSESPGTNPMATIMMLGRYVGMKMLQ
Sbjct: 481 TFWHFHGGCVVGKVVDGTYRVMGIENLRVVDGSTFSESPGTNPMATIMMLGRYVGMKMLQ 540

Query: 546 ERLSLA 551
           ERLSLA
Sbjct: 541 ERLSLA 546

BLAST of CsGy2G005902 vs. ExPASy TrEMBL
Match: A0A1S3BU55 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493189 PE=3 SV=1)

HSP 1 Score: 1044 bits (2700), Expect = 0.0
Identity = 518/551 (94.01%), Postives = 537/551 (97.46%), Query Frame = 0

Query: 1   MEHSTMSAFLLHILIFCCQSHLGVFSTSYTKLGNSDQDVSYMKFVHNANKFPEKEEYDYI 60
           MEHSTMSAFLLHILIF  QSHLGVFST+YTK  NS+QDVSY+KFVHNANK PEKEEYDYI
Sbjct: 1   MEHSTMSAFLLHILIFFFQSHLGVFSTTYTKPSNSNQDVSYLKFVHNANKLPEKEEYDYI 60

Query: 61  IIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQGLMNAFTDKDDGENPFQRFV 120
           IIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSV+NKQGLMNAFTDKD+GENPFQRFV
Sbjct: 61  IIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVMNKQGLMNAFTDKDNGENPFQRFV 120

Query: 121 SEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWDMEMVREAYEWVEETLVSEP 180
           SEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEI EWDMEMV +AYEWVEETLVS+P
Sbjct: 121 SEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEISEWDMEMVGKAYEWVEETLVSKP 180

Query: 181 NLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDNQGNRHGAVELLNKGESENL 240
           NLSSWQFAFRKALLEVG+DHDNGF+LRH++GTKIGGSIFDNQGNRHGAVELLNKGE ENL
Sbjct: 181 NLSSWQFAFRKALLEVGIDHDNGFDLRHVLGTKIGGSIFDNQGNRHGAVELLNKGEFENL 240

Query: 241 KVAVQATVKRILFSGLSANGVLYSDSKGKSHTAFIHEKGEIILSAGAIGSPQLLLLSGVG 300
           KVAVQATVKRILFSGLSANGVLYSDSKGK+HTAFIHEKGEIILSAGAIGSPQLLLLSG+G
Sbjct: 241 KVAVQATVKRILFSGLSANGVLYSDSKGKTHTAFIHEKGEIILSAGAIGSPQLLLLSGIG 300

Query: 301 STSHLSSLNLPLFLHQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLS 360
           STSHLSSL+LPL  HQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLS
Sbjct: 301 STSHLSSLDLPLVFHQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLS 360

Query: 361 SFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLGSLWLNSSTDVRKSPMVRFNYF 420
           SFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLG LWLNSSTDV+KSPMVRFNYF
Sbjct: 361 SFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLGFLWLNSSTDVQKSPMVRFNYF 420

Query: 421 SHPRDLAQCIGGLRKIQDLLNTQTIENIKTKDLEGKKTLQFLGIPLPENMADDTLVGEFC 480
           SHP DLAQCIGGLRKIQDLLNTQTIENIKT+DLEGKKTLQF+G+PLPENMADDTLVGEFC
Sbjct: 421 SHPHDLAQCIGGLRKIQDLLNTQTIENIKTQDLEGKKTLQFIGLPLPENMADDTLVGEFC 480

Query: 481 KRTVTTFWHFHGGCVVGKVVDGTYRVMGIENLRVVDGSTFSESPGTNPMATIMMLGRYVG 540
           KRTVTTFWHFHGGCVVGKVVDG YRVMGIENLRVVDGSTF ESPGTNPMATIMMLGRYVG
Sbjct: 481 KRTVTTFWHFHGGCVVGKVVDGNYRVMGIENLRVVDGSTFFESPGTNPMATIMMLGRYVG 540

Query: 541 MKMLQERLSLA 551
           MKMLQERL+LA
Sbjct: 541 MKMLQERLNLA 551

BLAST of CsGy2G005902 vs. ExPASy TrEMBL
Match: A0A5A7VNT0 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold418G00910 PE=3 SV=1)

HSP 1 Score: 1020 bits (2638), Expect = 0.0
Identity = 505/537 (94.04%), Postives = 523/537 (97.39%), Query Frame = 0

Query: 1   MEHSTMSAFLLHILIFCCQSHLGVFSTSYTKLGNSDQDVSYMKFVHNANKFPEKEEYDYI 60
           MEHSTMSAFLLHILIF  QSHLGVFST+YTK  NS+QDVSY+KFVHNANK PEKEEYDYI
Sbjct: 1   MEHSTMSAFLLHILIFFFQSHLGVFSTTYTKPSNSNQDVSYLKFVHNANKLPEKEEYDYI 60

Query: 61  IIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQGLMNAFTDKDDGENPFQRFV 120
           IIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSV+NKQGLMNAFTDKD+GENPFQRFV
Sbjct: 61  IIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVMNKQGLMNAFTDKDNGENPFQRFV 120

Query: 121 SEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWDMEMVREAYEWVEETLVSEP 180
           SEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEI EWDMEMV +AYEWVEETLVS+P
Sbjct: 121 SEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEISEWDMEMVGKAYEWVEETLVSKP 180

Query: 181 NLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDNQGNRHGAVELLNKGESENL 240
           NLSSWQFAFRKALLEVG+DHDNGF+LRH++GTKIGGSIFDNQGNRHGAVELLNKGE ENL
Sbjct: 181 NLSSWQFAFRKALLEVGIDHDNGFDLRHVLGTKIGGSIFDNQGNRHGAVELLNKGEFENL 240

Query: 241 KVAVQATVKRILFSGLSANGVLYSDSKGKSHTAFIHEKGEIILSAGAIGSPQLLLLSGVG 300
           KVAVQATVKRILFSGLSANGVLYSDSKGK+HTAFIHEKGEIILSAGAIGSPQLLLLSG+G
Sbjct: 241 KVAVQATVKRILFSGLSANGVLYSDSKGKTHTAFIHEKGEIILSAGAIGSPQLLLLSGIG 300

Query: 301 STSHLSSLNLPLFLHQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLS 360
           STSHLSSL+LPL  HQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLS
Sbjct: 301 STSHLSSLDLPLVFHQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLS 360

Query: 361 SFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLGSLWLNSSTDVRKSPMVRFNYF 420
           SFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLG LWLNSSTDV+KSPMVRFNYF
Sbjct: 361 SFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLGFLWLNSSTDVQKSPMVRFNYF 420

Query: 421 SHPRDLAQCIGGLRKIQDLLNTQTIENIKTKDLEGKKTLQFLGIPLPENMADDTLVGEFC 480
           SHP DLAQCIGGLRKIQDLLNTQTIENIKT+DLEGKKTLQF+G+PLPENMADDTLVGEFC
Sbjct: 421 SHPHDLAQCIGGLRKIQDLLNTQTIENIKTQDLEGKKTLQFIGLPLPENMADDTLVGEFC 480

Query: 481 KRTVTTFWHFHGGCVVGKVVDGTYRVMGIENLRVVDGSTFSESPGTNPMATIMMLGR 537
           KRTVTTFWHFHGGCVVGKVVDG YRVMGIENLRVVDGSTF ESPGTNPMATIMMLGR
Sbjct: 481 KRTVTTFWHFHGGCVVGKVVDGNYRVMGIENLRVVDGSTFFESPGTNPMATIMMLGR 537

BLAST of CsGy2G005902 vs. ExPASy TrEMBL
Match: A0A5D3CG26 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00550 PE=3 SV=1)

HSP 1 Score: 958 bits (2476), Expect = 0.0
Identity = 471/496 (94.96%), Postives = 486/496 (97.98%), Query Frame = 0

Query: 42  MKFVHNANKFPEKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQG 101
           MKFVHNANK PEKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSV+NKQG
Sbjct: 1   MKFVHNANKLPEKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVMNKQG 60

Query: 102 LMNAFTDKDDGENPFQRFVSEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWD 161
           LMNAFTDKD+GENPFQRFVSEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEI EWD
Sbjct: 61  LMNAFTDKDNGENPFQRFVSEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEISEWD 120

Query: 162 MEMVREAYEWVEETLVSEPNLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDN 221
           MEMV +AYEWVEETLVS+PNLSSWQFAFRKALLEVG+DHDNGF+LRH++GTKIGGSIFDN
Sbjct: 121 MEMVGKAYEWVEETLVSKPNLSSWQFAFRKALLEVGIDHDNGFDLRHVLGTKIGGSIFDN 180

Query: 222 QGNRHGAVELLNKGESENLKVAVQATVKRILFSGLSANGVLYSDSKGKSHTAFIHEKGEI 281
           QGNRHGAVELLNKGE ENLKVAVQATVKRILFSGLSANGVLYSDSKGK+HTAFIHEKGEI
Sbjct: 181 QGNRHGAVELLNKGEFENLKVAVQATVKRILFSGLSANGVLYSDSKGKTHTAFIHEKGEI 240

Query: 282 ILSAGAIGSPQLLLLSGVGSTSHLSSLNLPLFLHQPHVGQFMSDNPRFGVNIVLPFPLPT 341
           ILSAGAIGSPQLLLLSG+GSTSHLSSL+LPL  HQPHVGQFMSDNPRFGVNIVLPFPLPT
Sbjct: 241 ILSAGAIGSPQLLLLSGIGSTSHLSSLDLPLVFHQPHVGQFMSDNPRFGVNIVLPFPLPT 300

Query: 342 TTVEVVGILEKNTYFESLSSFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLGSL 401
           TTVEVVGILEKNTYFESLSSFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLG L
Sbjct: 301 TTVEVVGILEKNTYFESLSSFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLGCL 360

Query: 402 WLNSSTDVRKSPMVRFNYFSHPRDLAQCIGGLRKIQDLLNTQTIENIKTKDLEGKKTLQF 461
           WLNSSTDV+KSPMVRFNYFSHP DLAQCIGGLRKIQDLLNTQTIENIKT+DLEGKKTLQF
Sbjct: 361 WLNSSTDVQKSPMVRFNYFSHPHDLAQCIGGLRKIQDLLNTQTIENIKTQDLEGKKTLQF 420

Query: 462 LGIPLPENMADDTLVGEFCKRTVTTFWHFHGGCVVGKVVDGTYRVMGIENLRVVDGSTFS 521
           +G+PLPENMADDTLVGEFCKRTVTTFWHFHGGCVVGKVVDG YRVMGIENLRVVDGSTF 
Sbjct: 421 IGLPLPENMADDTLVGEFCKRTVTTFWHFHGGCVVGKVVDGNYRVMGIENLRVVDGSTFF 480

Query: 522 ESPGTNPMATIMMLGR 537
           ESPGTNPMATIMMLGR
Sbjct: 481 ESPGTNPMATIMMLGR 496

BLAST of CsGy2G005902 vs. ExPASy TrEMBL
Match: A0A6J1HMA6 ((R)-mandelonitrile lyase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465496 PE=3 SV=1)

HSP 1 Score: 825 bits (2130), Expect = 2.61e-297
Identity = 415/552 (75.18%), Postives = 470/552 (85.14%), Query Frame = 0

Query: 1   MEHSTMSAFLLHILIFCCQSHLGVFSTSYTKLGNSDQDVSYMKFVHNANKFPEKEE---Y 60
           M+HSTM+ FLL  LI              TK  N      YMKFVHNAN  P+ +E   Y
Sbjct: 1   MKHSTMATFLLLTLISISVD---------TKPINP-----YMKFVHNANNIPDHQEQQPY 60

Query: 61  DYIIIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQGLMNAFTDKDDGENPFQ 120
           DYI+IGGGTAGCPLA TLS+ F+VLLLERG+ P KFPSVLNKQGL+NAFTD DDG+ P++
Sbjct: 61  DYIVIGGGTAGCPLATTLSTNFTVLLLERGSEPTKFPSVLNKQGLLNAFTDDDDGKGPYE 120

Query: 121 RFVSEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWDMEMVREAYEWVEETLV 180
           RFVSEDGVENLRGRILGGSSMINAGFYSRAH+EFFE+  + EW+MEMV + YEWVEET+V
Sbjct: 121 RFVSEDGVENLRGRILGGSSMINAGFYSRAHREFFESGGV-EWEMEMVEKGYEWVEETVV 180

Query: 181 SEPNLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDNQGNRHGAVELLNKGES 240
           S P+LSSWQ A R+A+LE GV  DNGF+LR +VGTK GGSIFD  GNR GAVE LNK ES
Sbjct: 181 SRPSLSSWQAALRRAMLEGGVGPDNGFDLRSVVGTKTGGSIFDEHGNRRGAVEFLNKVES 240

Query: 241 ENLKVAVQATVKRILFSGLSANGVLYSDSKGKSHTAFIHEKGEIILSAGAIGSPQLLLLS 300
            NLKV V+A+V RI+FSGLSA GV YSDSKGKSHTA+I +KGEIILSAGAIGSPQLLLLS
Sbjct: 241 NNLKVVVEASVHRIIFSGLSATGVSYSDSKGKSHTAYIRKKGEIILSAGAIGSPQLLLLS 300

Query: 301 GVGSTSHLSSLNLPLFLHQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFE 360
           G+G  SHLSSLNLP+ LHQPHVG+FMSDNPRFGVNI+LPFPLPTTTVEVVG  E NT+FE
Sbjct: 301 GIGPISHLSSLNLPVVLHQPHVGEFMSDNPRFGVNIMLPFPLPTTTVEVVGTTETNTHFE 360

Query: 361 SLSSFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLGSLWLNSSTDVRKSPMVRF 420
           SLS+F+PFSIPPSF+LLPP STSLN+SLV+ISGKFSKV S+GSL LNSSTDVR+SP VRF
Sbjct: 361 SLSNFLPFSIPPSFALLPPHSTSLNLSLVVISGKFSKVGSVGSLRLNSSTDVRRSPTVRF 420

Query: 421 NYFSHPRDLAQCIGGLRKIQDLLNTQTIENIKTKDLEGKKTLQFLGIPLPENMADDTLVG 480
           NYF+ P DLAQC+ GLRKI DLLNTQT+E IKT DLEGKK +QFLG+PLPENMADD LVG
Sbjct: 421 NYFTQPEDLAQCVKGLRKIGDLLNTQTMEKIKTADLEGKKRVQFLGVPLPENMADDGLVG 480

Query: 481 EFCKRTVTTFWHFHGGCVVGKVVDGTYRVMGIENLRVVDGSTFSESPGTNPMATIMMLGR 540
           EFC++TVTTFWH+HGGC+VGKVVDG YRV+G+E LRVVDGSTFS+SPGTNPMAT+ MLGR
Sbjct: 481 EFCRKTVTTFWHYHGGCLVGKVVDGNYRVVGVEKLRVVDGSTFSDSPGTNPMATVSMLGR 537

Query: 541 YVGMKMLQERLS 549
           YVG+KML+ERLS
Sbjct: 541 YVGLKMLRERLS 537

BLAST of CsGy2G005902 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 536.2 bits (1380), Expect = 3.1e-152
Identity = 262/516 (50.78%), Postives = 362/516 (70.16%), Query Frame = 0

Query: 41  YMKFVHNANKFPEKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQ 100
           +M+F+ NA  F  ++ YDYII+GGGTAGCPLAATLS  F VLLLERG +P   P+V++  
Sbjct: 38  FMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSHD 97

Query: 101 GLMNAFTDKDDGENPFQRFVSEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEW 160
           G +   TD ++ ++P Q F+SE+GV N RGR+LGGSS INAGFYSRA K+FFE   ++ W
Sbjct: 98  GFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGLV-W 157

Query: 161 DMEMVREAYEWVEETLVSEPNLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFD 220
           D+  V ++YEWVE  +V  P L +WQ A R ALLEVGV   NGF L H VGTKIGGS FD
Sbjct: 158 DLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFD 217

Query: 221 NQGNRHGAVELLNKGESENLKVAVQATVKRILF--------SGLSANGVLYSDSKGKSHT 280
             G RH + +LL    S N++VAV ATV+R+L         S +SA GV+Y D  G+ H 
Sbjct: 218 RTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHH 277

Query: 281 AFIHEKGEIILSAGAIGSPQLLLLSGVGSTSHLSSLNLPLFLHQPHVGQFMSDNPRFGVN 340
           A I ++GE+ILSAGA+GSPQLL LSG+G  S+LS+  +P+ L QPHVG F+ DNPR G++
Sbjct: 278 ALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGIS 337

Query: 341 IVLPFPLPTTTVEVVGILEKNTYFESLSSFIPFSIPPSFSLLPPQSTSLNMSLVLISGKF 400
           IV P P+  + ++VVG+ E   + E+ S+ IPF+ P     +   ++ L + +  I  K 
Sbjct: 338 IVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVPVTTIMEKI 397

Query: 401 SKVDSLGSLWLNSSTDVRKSPMVRFNYFSHPRDLAQCIGGLRKIQDLLNTQTIENIKTKD 460
               S+G L L +STDVR +P+VRFNYFS P+DL +C+ G RKI ++L ++ +++   ++
Sbjct: 398 LGPVSIGLLRL-ASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIRE 457

Query: 461 LEGKKTLQFLGIPLPENMADDTLVGEFCKRTVTTFWHFHGGCVVGKVVDGTYRVMGIENL 520
             G +  +F+G PLP + ++D ++ +FC+RTV+T WH+HGG VVGKVVD   +V+G+ +L
Sbjct: 458 WFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSL 517

Query: 521 RVVDGSTFSESPGTNPMATIMMLGRYVGMKMLQERL 549
           R+VDGSTF+ SPGTNP AT+MMLGRY+G+KML+ER+
Sbjct: 518 RLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRERM 551

BLAST of CsGy2G005902 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 409.1 bits (1050), Expect = 5.8e-114
Identity = 235/560 (41.96%), Postives = 323/560 (57.68%), Query Frame = 0

Query: 29  YTKLGNSDQDVSYMKFVHNANKFPEKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGN 88
           ++ + +SD+  +Y  F+ +A   P    YDYIIIGGGTAGCPLAATLS   SVLLLERG+
Sbjct: 19  HSPICSSDKAPNY-SFMRDATGSPTTSYYDYIIIGGGTAGCPLAATLSQNASVLLLERGD 78

Query: 89  IPNKFPSVLNKQGLMNAFTDKDDGENPFQRFVSEDGVENLRGRILGGSSMINAGFYSRAH 148
            P   P++        A +D  +  +P QRFVSEDGV N R R+LGG S +NAGFY+RA 
Sbjct: 79  SPYNNPNITRLSAFGAALSDLSE-SSPSQRFVSEDGVINARARVLGGGSALNAGFYTRAG 138

Query: 149 KEFFETQEIIEWDMEMVREAYEWVEETLVSEPNLSSWQFAFRKALLEVGVDHDNGFELRH 208
            ++      + WD  +  E+Y+WVE  +  +P +  WQ A R  LLE G+  +NGF   H
Sbjct: 139 TKYVRN---MGWDGALANESYQWVEAKVAFQPPMGRWQTAVRDGLLEAGIVPNNGFTYDH 198

Query: 209 LVGTKIGGSIFDNQGNRHGAVELLNKGESENLKVAVQATVKRILFSGLS-----ANGVLY 268
           + GTK GG+IFD  GNRH A +LL   + + + V + ATV RILF         ANGV+Y
Sbjct: 199 INGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILFRTRGTTKPIANGVVY 258

Query: 269 SDSKGKSHTAFIHE--KGEIILSAGAIGSPQLLLLSGVGSTSHLSSLNLPLFLHQPHVGQ 328
            D  G++H A++ E    EIILSAG +GSPQLL+LSGVG ++ L + N+ + + QPHVGQ
Sbjct: 259 RDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVVMDQPHVGQ 318

Query: 329 FMSDNPRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLSS-------------------F 388
            M DNP   V +  P P+  + +EVVGI  + TY E+                      +
Sbjct: 319 GMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGGGGGSSGSSSTRDYY 378

Query: 389 IPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKVDSLGSLWLN----SSTDVRKSPMVRFN 448
             FS  P  +LL   S +   S     G F     +G L        + + + +P+V FN
Sbjct: 379 AMFS--PRATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLELKTRNPKDNPIVTFN 438

Query: 449 YFSHPRDLAQCIGGLRKIQDLLNTQTIENIKTKDLEGKKTLQFLGIPLPENMAD------ 508
           YF HP DL +C+ G++ I+ ++ ++     K  D+  +  L  L    P N+        
Sbjct: 439 YFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADVSFEYLLN-LTASTPVNLRPPRSGPG 498

Query: 509 ---DTLVGEFCKRTVTTFWHFHGGCVVGKVVDGTYRVMGIENLRVVDGSTFSESPGTNPM 550
                   EFC+ TVTT WH+HGGCVVG+VVDG Y+V+GI+ LRV+D ST    PGTNP 
Sbjct: 499 ASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMSTVGYCPGTNPQ 558

BLAST of CsGy2G005902 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 389.0 bits (998), Expect = 6.2e-108
Identity = 227/537 (42.27%), Postives = 310/537 (57.73%), Query Frame = 0

Query: 53  EKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQGLMNAFTDKDDG 112
           +   YDYI+IGGGTAGCPLAATLS  FSVL+LERG +P    +V   +       D    
Sbjct: 59  QDSSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRNFHIGLAD-ISA 118

Query: 113 ENPFQRFVSEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWDMEMVREAYEWV 172
            +  Q FVS DGV N R R+LGG S INAGFYSRA   F +      WD ++V+E+Y WV
Sbjct: 119 SSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKR---AGWDPKLVKESYPWV 178

Query: 173 EETLVSEPNLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDNQGNRHGAVELL 232
           E  +V +P L+ WQ A R +LLEVGV   NGF   H+ GTKIGG+IFD  G RH A ELL
Sbjct: 179 EREIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELL 238

Query: 233 NKGESENLKVAVQATVKRILF--SGL--SANGVLYSDSKGKSHTAFIHEK--GEIILSAG 292
                + L+V + ATV++I+F  SG      GV++ D KG  H A +  +   E+ILS+G
Sbjct: 239 AYANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSG 298

Query: 293 AIGSPQLLLLSGVGSTSHLSSLNLPLFLHQPHVGQFMSDNPRFGVNIVLPFPLPTTTVEV 352
           AIGSPQ+L+LSG+G    L  L +P+ L   HVG+ M+DNP   + +    P+  + ++ 
Sbjct: 299 AIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQT 358

Query: 353 VGILEKNTYFESLSSF--IPFSIPPS----------FSLLP-----PQST---------- 412
           VGI +   Y E+ + F   P SI             FS +P     P++T          
Sbjct: 359 VGITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQ 418

Query: 413 ---SLNMSLVLISGKFSKVDSLGSLWLNSSTDVRKSPMVRFNYFSHPRDLAQCIGGLRKI 472
              + N S +L   K +   S G L L  +T+V  +P V FNYF HP DL +C+  +R +
Sbjct: 419 LHEAFNGSFIL--EKLAYPISRGHLSL-VNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLV 478

Query: 473 QDLLNTQTIENIKTKDLEGKKTLQFLGIPL-----PENMADDTLVGEFCKRTVTTFWHFH 532
             ++ +    N    D +    +  L +       P+ + D   + +FCK TV T WH+H
Sbjct: 479 SKVVTSNRFLNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYH 538

Query: 533 GGCVVGKVVDGTYRVMGIENLRVVDGSTFSESPGTNPMATIMMLGRYVGMKMLQERL 549
           GGC+VGKVV    +V+G++ LRV+DGSTF ESPGTNP AT+MM+GRY+G+K+L+ERL
Sbjct: 539 GGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRERL 588

BLAST of CsGy2G005902 vs. TAIR 10
Match: AT5G51930.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 382.5 bits (981), Expect = 5.8e-106
Identity = 230/529 (43.48%), Postives = 311/529 (58.79%), Query Frame = 0

Query: 44  FVHNANKFPEKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPSVLNKQGLM 103
           F+ +A   P+   +DYIIIGGGTAGC LAATLS   SVL+LERG  P + P+  +    +
Sbjct: 60  FMKDATLAPKNASFDYIIIGGGTAGCALAATLSQNASVLVLERGGSPYENPTATDMGNSV 119

Query: 104 NAFTDKDDGENPFQRFVSEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQEIIEWDME 163
           N   + +   +  Q F+SEDGV N R R+LGG S+IN GFYSRA  ++ E     EW+ME
Sbjct: 120 NTLLN-NTPNSWSQLFISEDGVYNTRPRVLGGGSVINGGFYSRAGNDYVEE---AEWEME 179

Query: 164 MVREAYEWVEETLVSEPNLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIGGSIFDNQG 223
            V  AYEWVE+ LV EP +  WQ AF+  LLE G   DNGF   H+ GTKIGG+IFD  G
Sbjct: 180 EVEAAYEWVEKKLVFEPQVIEWQKAFKDGLLEAGESPDNGFTYDHIYGTKIGGTIFDRAG 239

Query: 224 NRHGAVELLNKGESENLKVAVQATVKRILFSGLSANGVLYSDSKGKSHTAFIHEK--GEI 283
           +RH A  LL       + V + A+V ++LF+   A  VL+ D+ G  H A +  K   E+
Sbjct: 240 HRHTAANLLEYANPNRIVVYLHASVHKVLFT-TEAYEVLFEDANGVFHKANLANKATNEV 299

Query: 284 ILSAGAIGSPQLLLLSGVGSTSHLSSLNL-PLFLHQPHVGQFMSDNPRFGVNIVLPFPLP 343
           ILSAGA+GSPQLL+LSGVG   HL +  + PL L QP VGQ M+DNP   V I  P P+ 
Sbjct: 300 ILSAGALGSPQLLMLSGVGPAVHLEAHGVNPLVLDQPMVGQGMADNPMNFVAIPSPQPVE 359

Query: 344 TTTVEVVGILEKNTYFESLSSF-IPFSIPPSF-----SLLPPQSTSLNMSL--------- 403
            + ++ VGI + ++Y E LS   + F I   F     +LL   S + +  +         
Sbjct: 360 LSLIQAVGITKFDSYIEGLSGLSLSFDITRRFFDGVLNLLNETSHTTSRKILTQSIAVLL 419

Query: 404 --------VLISGK--FSKVD---SLGSLWLNSSTDVRKSPMVRFNYFSHPRDLAQCIGG 463
                   V ++G   F KVD   S G + L  +T+ R +P V FNY+  P DL +C+ G
Sbjct: 420 KSFDVKLEVRMNGGLIFQKVDGPASKGHMKLR-NTNPRDNPSVTFNYYQEPEDLNKCVKG 479

Query: 464 LRKIQDLLNTQTIENIKTKDLEGKKTLQ-FLGIPL---PENMADDTLVGEFCKRTVTTFW 523
           L  I  ++N++     K   +  ++ L   L +P+   P ++     + +FC  TVT+ W
Sbjct: 480 LNTIIRMINSKAFSKYKYPGVTARELLNLMLALPINLRPRHVTSAFNLKQFCIDTVTSVW 539

Query: 524 HFHGGCVVGKVVDGTYRVMGIENLRVVDGSTFSESPGTNPMATIMMLGR 538
           H+HGGC VGKVVD  Y+V+GI+ LRV+DGSTF +SPGTNP AT+MMLGR
Sbjct: 540 HYHGGCQVGKVVDKNYKVLGIDGLRVIDGSTFLKSPGTNPQATVMMLGR 582

BLAST of CsGy2G005902 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 374.8 bits (961), Expect = 1.2e-103
Identity = 227/563 (40.32%), Postives = 313/563 (55.60%), Query Frame = 0

Query: 35  SDQDVSYMKFVHNANKFPEKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFP 94
           S     Y  F+ +A   P    +DYIIIGGGT+GC LAATLS   SVL+LERG  P   P
Sbjct: 24  SPDKAGYYSFMKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNP 83

Query: 95  SVLNKQGLMNAFTDKDDGENPFQRFVSEDGVENLRGRILGGSSMINAGFYSRAHKEFFET 154
           +  + +      ++    ++  Q F+SEDGV N R R+LGG S++NAGFY+RA  E+ + 
Sbjct: 84  TATDIENFATTLSNTSP-KSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVKE 143

Query: 155 QEIIEWDMEMVREAYEWVEETLVSEPNLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKI 214
               EW  + V  AYEWVE+ +  +P +  WQ AF+  LLE G    NGF   H+ GTKI
Sbjct: 144 ---TEWKTDEVEAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKI 203

Query: 215 GGSIFDNQGNRHGAVELLNKGESENLKVAVQATVKRILFSGL-----SANGVLYSDSKGK 274
           GG+IFD  G+RH A +LL      N+ V + A+V +ILF+        A GV++ D+ G 
Sbjct: 204 GGTIFDRAGHRHTAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGV 263

Query: 275 SHTAFIHEK--GEIILSAGAIGSPQLLLLSGVGSTSHLSSLNL-PLFLHQPHVGQFMSDN 334
            H A + +    E+ILSAGAIGSPQLL+LSG+G  +HL++  + PL L  P VGQ M DN
Sbjct: 264 LHKAELEKNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDN 323

Query: 335 PRFGVNIVLPFPLPTTTVEVVGILEKNTYFESLSSFI----------------------- 394
           P   + I  P P+  + ++VVGI +  +Y E  S  I                       
Sbjct: 324 PMNAIFIPSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLNEMQTS 383

Query: 395 -----------PFSIPPSFSLLPP---QSTSLNMSLVLISGKFSKVDSLGSLWLNSSTDV 454
                        SI   F+ + P    +T   + L  I+G  S+    G L L  +T+ 
Sbjct: 384 RTTSTTSPTLSTQSITDFFNPINPLLNATTRAGLILQKIAGPISR----GHLELR-NTNP 443

Query: 455 RKSPMVRFNYFSHPRDLAQCIGGLRKIQDLLNTQTIENIKTKD--LEGKKTLQFLGIPL- 514
             +P VRFNY+  P DL  C+ G+  I  ++N++     K  D  + G   L  L +P  
Sbjct: 444 DDNPSVRFNYYQEPEDLQICVEGINTIIKVINSKAFSKFKYPDATIHGLLDL-MLSVPTN 503

Query: 515 --PENMADDTLVGEFCKRTVTTFWHFHGGCVVGKVVDGTYRVMGIENLRVVDGSTFSESP 548
             P ++     + +FC  TV T WH+HGGC VG+VVD  YRV+GI++LRV+DGSTF +SP
Sbjct: 504 LRPRHITSMFNLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSP 563

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P527062.3e-15550.09(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1[more]
Q945K25.6e-15450.27(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1[more]
Q9SSM24.4e-15150.78(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ... [more]
P527076.4e-15049.82(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1[more]
O242432.0e-14849.82(R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAE8651657.10.0100.00hypothetical protein Csa_021352 [Cucumis sativus][more]
XP_008452076.10.094.01PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo][more]
KAA0067271.10.094.04(R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa][more]
XP_031736690.10.096.59(R)-mandelonitrile lyase-like [Cucumis sativus][more]
TYK10883.10.094.96(R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A0A0LMB30.0100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G050000 PE=3 SV=1[more]
A0A1S3BU550.094.01(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493189 PE=3 SV=... [more]
A0A5A7VNT00.094.04(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A5D3CG260.094.96(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A6J1HMA62.61e-29775.18(R)-mandelonitrile lyase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465496 PE=3... [more]
Match NameE-valueIdentityDescription
AT1G73050.13.1e-15250.78Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G12570.15.8e-11441.96Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.16.2e-10842.27Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51930.15.8e-10643.48Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51950.11.2e-10340.32Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 57..326
e-value: 2.4E-31
score: 109.2
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00623GMC_OXRED_1coord: 130..153
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00624GMC_OXRED_2coord: 286..300
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 399..537
e-value: 3.4E-28
score: 98.9
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 57..536
e-value: 2.4E-167
score: 559.7
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 48..546
IPR012132Glucose-methanol-choline oxidoreductasePIRSFPIRSF000137Alcohol_oxidasecoord: 366..550
e-value: 1.3E-27
score: 94.8
coord: 42..362
e-value: 4.0E-58
score: 195.5
NoneNo IPR availableGENE3D3.30.410.40coord: 178..486
e-value: 2.4E-167
score: 559.7
NoneNo IPR availablePANTHERPTHR45968OSJNBA0019K04.7 PROTEINcoord: 1..549
NoneNo IPR availablePANTHERPTHR45968:SF23(R)-MANDELONITRILE LYASE 4-RELATEDcoord: 1..549
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 323..493

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy2G005902.1CsGy2G005902.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors