CsGy1G032130 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy1G032130
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionProtein kinase domain-containing protein
LocationGy14Chr1: 31369134 .. 31371059 (-)
RNA-Seq ExpressionCsGy1G032130
SyntenyCsGy1G032130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATACCAAAAGAAAGAATATTCTAAATAAGGATCCAATATCCTCTAAGCCTTCTATAAAAATCCCCCAATTCCCAACTGATTCCCCCAAAAGCACTCCTACTTCCTAAGACTACAGAGAAAGAGAGAGAGAGAGAGAGAGAGAAAGAGAGAAAGAGACAAACAAGTTCAAGAATTTTGTAGAGAGCAAATACCAAAATCCCCCAATTTTTCCAAAGAAGAAGAACAAACAAATCTTCAGCAATGGCCGACATGATCGTCAACAGCCAATACCAAATCCTCGAGGAGCTCGGAAGAGGAAGGTTCGGAATCATCACTCGTTGCTTTTGTCCAATCTCCAACAAGTTCTTCGCCTGCAAAACCATCTCTAAAAACGATCTCAACGATGAAACTGACAAAGAATGCCTTGTCAAAGAACCCAAAATCATGGCACTTCTCCCTTCCCATCCCAACATTCTTCAGTTAATCGACGTTCTCGAGAATGACCATTACCTCTGCTTGATTTCCGACCTCTGCGAATCCCTCTCCCTTTACGATCGGATCATCCGTCGTCCCTTTTCCGAATCCGAAGCTGCCGTCGTCATGAAGCAGCTTCTCCAAGCCCTCGCCCACTGTCATTCTCATGGCGTCGTTCATCGCGATGTCAAGCCTGACAATGTGCTCTTCGATTCCAGGAACAATTTGAAACTCATCGATTTCGGATCGGCTGAGTGGTGTGATGAAGATGGATTTATGTACGGTGTCGTCGGAACGCCTTATTACGTTGCGCCGGAAGTTTTGAGAGGATCTGAGTACGGTCGGAAGGTCGACGTCTGGAGCGCCGGTGTGATTCTTTACACGATGTTGGCCGGATTTCCCCCGTTCTACGGTGAATCTGCGGCGGAGGTCTTTGAGGCTGTTCTTAGAGGAAATCTCAGATTTCCGACTAGGGTTTTTAGGTCGGTTTCTTCAGCGGCTAAGGATCTGATGAAGAAGATGATCTGTCGAGATGTTTCTAGAAGATTCTCAGCCGAACAAGCTCTCAGTAAGTCTAATTCTTATATTCAATCAAACCATTTTATGTTCTTGATCTGGTTGTTCTGGCAACTGTTTATTGATTGTTTCCATATTTTTTTAGCAAATGGTTGAACCATTTGAAGAAACCCCAAAACCCCAAGTGAAAATTCATTGCATATTTCAAAACTAGTGAATCTATTTGTTTTCTTATGGGGGTTGGAAACTGTTGTTTTGCAGGACACCCATGGATATTGAGTGGAGGAGAAGCAACATCTTCAGTTGAATAATTTGATCTTAACACTTTTTAACTCATCCCTAAAAAGAGAAACATATGAAGAAAGGATGCTATTGAAAAAGAATCAAGCATTCCTATTTTTGTATTCAAGTACATAATCCCCTGCTCTGCTTCTTCACCATATTGTGAATAAGAGACCTTAACCTTCAGTTCAGACTCCATCAATGGCTGCCTGCTCCATGTATAAGGTTCTAGAGAGAGAGAAAGATAACATCAGTTGTTGATTTGAGACTCCGCTGCTGTCAATATCATCTTTCTTTTCTTTTTTGATTTCTTCAATGCTTTGAGAAGCATTTGAGGATGTTTTTTTTTCTTTTTTTGCCTTTTTCATTTTGATTTGTTGTTAGGTAAAAACGGAATTTGGGGAATCCTTTCTTTCCCTCCCAACCCTTTTCTTTTTGTTGTATAAAGTGTTTGGGAAGTGAGAAAGTCCCACAAAAAAAATGGGAAAAGAATTAGAAAAAGGAGAGAGAAAAATAGTGAGAGAGAGATAAAGAAGAGAAGTTGTATTCAAATTAGTAGATGGCTTTTTTGTTTTGTTTCTTTTTTTTCAATTTTCAAGGATGTAATGAAATGTGATGTGATGTGGATGCAATTTGCTGGGAATATAAGGGTGATGTTTTAGTGTAAGTTTGCTC

mRNA sequence

ATACCAAAAGAAAGAATATTCTAAATAAGGATCCAATATCCTCTAAGCCTTCTATAAAAATCCCCCAATTCCCAACTGATTCCCCCAAAAGCACTCCTACTTCCTAAGACTACAGAGAAAGAGAGAGAGAGAGAGAGAGAGAAAGAGAGAAAGAGACAAACAAGTTCAAGAATTTTGTAGAGAGCAAATACCAAAATCCCCCAATTTTTCCAAAGAAGAAGAACAAACAAATCTTCAGCAATGGCCGACATGATCGTCAACAGCCAATACCAAATCCTCGAGGAGCTCGGAAGAGGAAGGTTCGGAATCATCACTCGTTGCTTTTGTCCAATCTCCAACAAGTTCTTCGCCTGCAAAACCATCTCTAAAAACGATCTCAACGATGAAACTGACAAAGAATGCCTTGTCAAAGAACCCAAAATCATGGCACTTCTCCCTTCCCATCCCAACATTCTTCAGTTAATCGACGTTCTCGAGAATGACCATTACCTCTGCTTGATTTCCGACCTCTGCGAATCCCTCTCCCTTTACGATCGGATCATCCGTCGTCCCTTTTCCGAATCCGAAGCTGCCGTCGTCATGAAGCAGCTTCTCCAAGCCCTCGCCCACTGTCATTCTCATGGCGTCGTTCATCGCGATGTCAAGCCTGACAATGTGCTCTTCGATTCCAGGAACAATTTGAAACTCATCGATTTCGGATCGGCTGAGTGGTGTGATGAAGATGGATTTATGTACGGTGTCGTCGGAACGCCTTATTACGTTGCGCCGGAAGTTTTGAGAGGATCTGAGTACGGTCGGAAGGTCGACGTCTGGAGCGCCGGTGTGATTCTTTACACGATGTTGGCCGGATTTCCCCCGTTCTACGGTGAATCTGCGGCGGAGGTCTTTGAGGCTGTTCTTAGAGGAAATCTCAGATTTCCGACTAGGGTTTTTAGGTCGGTTTCTTCAGCGGCTAAGGATCTGATGAAGAAGATGATCTGTCGAGATGTTTCTAGAAGATTCTCAGCCGAACAAGCTCTCAGACACCCATGGATATTGAGTGGAGGAGAAGCAACATCTTCAGTTGAATAATTTGATCTTAACACTTTTTAACTCATCCCTAAAAAGAGAAACATATGAAGAAAGGATGCTATTGAAAAAGAATCAAGCATTCCTATTTTTGTATTCAAGTACATAATCCCCTGCTCTGCTTCTTCACCATATTGTGAATAAGAGACCTTAACCTTCAGTTCAGACTCCATCAATGGCTGCCTGCTCCATGTATAAGGTTCTAGAGAGAGAGAAAGATAACATCAGTTGTTGATTTGAGACTCCGCTGCTGTCAATATCATCTTTCTTTTCTTTTTTGATTTCTTCAATGCTTTGAGAAGCATTTGAGGATGTTTTTTTTTCTTTTTTTGCCTTTTTCATTTTGATTTGTTGTTAGGTAAAAACGGAATTTGGGGAATCCTTTCTTTCCCTCCCAACCCTTTTCTTTTTGTTGTATAAAGTGTTTGGGAAGTGAGAAAGTCCCACAAAAAAAATGGGAAAAGAATTAGAAAAAGGAGAGAGAAAAATAGTGAGAGAGAGATAAAGAAGAGAAGTTGTATTCAAATTAGTAGATGGCTTTTTTGTTTTGTTTCTTTTTTTTCAATTTTCAAGGATGTAATGAAATGTGATGTGATGTGGATGCAATTTGCTGGGAATATAAGGGTGATGTTTTAGTGTAAGTTTGCTC

Coding sequence (CDS)

ATGGCCGACATGATCGTCAACAGCCAATACCAAATCCTCGAGGAGCTCGGAAGAGGAAGGTTCGGAATCATCACTCGTTGCTTTTGTCCAATCTCCAACAAGTTCTTCGCCTGCAAAACCATCTCTAAAAACGATCTCAACGATGAAACTGACAAAGAATGCCTTGTCAAAGAACCCAAAATCATGGCACTTCTCCCTTCCCATCCCAACATTCTTCAGTTAATCGACGTTCTCGAGAATGACCATTACCTCTGCTTGATTTCCGACCTCTGCGAATCCCTCTCCCTTTACGATCGGATCATCCGTCGTCCCTTTTCCGAATCCGAAGCTGCCGTCGTCATGAAGCAGCTTCTCCAAGCCCTCGCCCACTGTCATTCTCATGGCGTCGTTCATCGCGATGTCAAGCCTGACAATGTGCTCTTCGATTCCAGGAACAATTTGAAACTCATCGATTTCGGATCGGCTGAGTGGTGTGATGAAGATGGATTTATGTACGGTGTCGTCGGAACGCCTTATTACGTTGCGCCGGAAGTTTTGAGAGGATCTGAGTACGGTCGGAAGGTCGACGTCTGGAGCGCCGGTGTGATTCTTTACACGATGTTGGCCGGATTTCCCCCGTTCTACGGTGAATCTGCGGCGGAGGTCTTTGAGGCTGTTCTTAGAGGAAATCTCAGATTTCCGACTAGGGTTTTTAGGTCGGTTTCTTCAGCGGCTAAGGATCTGATGAAGAAGATGATCTGTCGAGATGTTTCTAGAAGATTCTCAGCCGAACAAGCTCTCAGACACCCATGGATATTGAGTGGAGGAGAAGCAACATCTTCAGTTGAATAA

Protein sequence

MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALLPSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQALAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRGSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE*
Homology
BLAST of CsGy1G032130 vs. ExPASy Swiss-Prot
Match: Q9SPK4 (Phosphoenolpyruvate carboxylase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PPCK1 PE=1 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 2.3e-92
Identity = 161/266 (60.53%), Postives = 203/266 (76.32%), Query Frame = 0

Query: 8   SQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALLPS 67
           ++YQI EE+GRGRFG ++R + P +  FFACKTI K  L+D+ D+ CL  EPK+MALL  
Sbjct: 13  NKYQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLSDDLDRACLDNEPKLMALLSY 72

Query: 68  HPNILQLIDVLENDHYLCLISDLCE-SLSLYDRIIRR-PFSESEAAVVMKQLLQALAHCH 127
           HPNI+Q+ D+++ D  L +  +L   S+S+YDR++    F E + A   KQ+LQAL+HCH
Sbjct: 73  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 132

Query: 128 SHGVVHRDVKPDNVLFDSRNN-LKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRGSEY 187
            +GVVHRD+KP+N+L D RN+ +K+ DFGS  W  E     GVVGTPYYVAPEVL G  Y
Sbjct: 133 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEGETTEGVVGTPYYVAPEVLMGYSY 192

Query: 188 GRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKK 247
           G KVD+WSAGV+LYTMLAG PPFYGE+A E+FEAVLRGNLRFPT++FR VSS AKD ++K
Sbjct: 193 GEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLRK 252

Query: 248 MICRDVSRRFSAEQALRHPWILSGGE 271
           +IC+D SRRFSAEQALRHPWI   GE
Sbjct: 253 LICKDASRRFSAEQALRHPWIQRAGE 278

BLAST of CsGy1G032130 vs. ExPASy Swiss-Prot
Match: Q93VK0 (Phosphoenolpyruvate carboxylase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PPCK2 PE=1 SV=2)

HSP 1 Score: 320.5 bits (820), Expect = 1.9e-86
Identity = 156/273 (57.14%), Postives = 202/273 (73.99%), Query Frame = 0

Query: 6   VNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALL 65
           + + YQ+ +E+GRGRFG ITRCF P + +F+ACKTI K  L D  D+EC+  EP+IMA+L
Sbjct: 7   LENNYQLCDEIGRGRFGTITRCFSPATKEFYACKTIDKRVLIDALDRECIETEPRIMAML 66

Query: 66  PSHPNILQLIDVLENDHYLCLISDLCE-SLSLYDRIIRR--PFSESEAAVVMKQLLQALA 125
           P HPNI+++ D+ E +  L ++ +L +  +++YDR+I      SESE+A   KQ+L ALA
Sbjct: 67  PPHPNIIRIFDLYETEDSLAIVMELVDPPMTIYDRLISAGGRLSESESASYAKQILSALA 126

Query: 126 HCHSHGVVHRDVKPDNVLFD-SRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRG 185
           HCH   VVHRDVKPDNVL D     +KL DFGSA W   +    GVVGTPYYVAPEV+ G
Sbjct: 127 HCHRCDVVHRDVKPDNVLVDLVSGGVKLCDFGSAVWLGGE-TAEGVVGTPYYVAPEVVMG 186

Query: 186 SEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
            +Y  KVD+WSAGV++YTMLAG PPF GE+A ++FE++LRGNLRFP + F SVSS AKDL
Sbjct: 187 RKYDEKVDIWSAGVVIYTMLAGEPPFNGETAEDIFESILRGNLRFPPKKFGSVSSEAKDL 246

Query: 246 MKKMICRDVSRRFSAEQALRHPWILSGGEATSS 275
           ++KMICRDVSRRFSAE ALRH W+++ G   S+
Sbjct: 247 LRKMICRDVSRRFSAEDALRHSWMMNVGNLQSN 278

BLAST of CsGy1G032130 vs. ExPASy Swiss-Prot
Match: P28582 (Calcium-dependent protein kinase OS=Daucus carota OX=4039 PE=2 SV=2)

HSP 1 Score: 226.5 bits (576), Expect = 3.7e-58
Identity = 113/271 (41.70%), Postives = 176/271 (64.94%), Query Frame = 0

Query: 6   VNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALL 65
           +  +Y + +ELGRG+FG + +C    S + +ACK+I K  L  + DKE + +E +I+  L
Sbjct: 77  IRGKYTLGKELGRGQFGCVYQCTENSSGQLYACKSILKRKLVSKNDKEDIKREIQILQHL 136

Query: 66  PSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRR-PFSESEAAVVMKQLLQALAHC 125
              PNI++   V E+   + L+ +LC    L+DRII +  +SE  AA + +Q++  +  C
Sbjct: 137 SGQPNIVEFKGVFEDRQSVHLVMELCAGGELFDRIIAQGHYSERAAATICRQIVNVVHVC 196

Query: 126 HSHGVVHRDVKPDNVLFDSRNN---LKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRG 185
           H  GV+HRD+KP+N L  S++    LK  DFG + + +E      +VG+ YYVAPEVLR 
Sbjct: 197 HFMGVMHRDLKPENFLLSSKDKDAMLKATDFGLSVFIEEGKVYRNIVGSAYYVAPEVLRR 256

Query: 186 SEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
           S YG+++D+WSAGVILY +L+G PPF+ E+   +F+A+L G + F +  + SVS++AKDL
Sbjct: 257 S-YGKEIDIWSAGVILYILLSGVPPFWAENEKGIFDAILEGVIDFESEPWPSVSNSAKDL 316

Query: 246 MKKMICRDVSRRFSAEQALRHPWILSGGEAT 273
           ++KM+ +D  RR ++ Q L HPW+  GGEA+
Sbjct: 317 VRKMLTQDPRRRITSAQVLDHPWMREGGEAS 346

BLAST of CsGy1G032130 vs. ExPASy Swiss-Prot
Match: P93759 (Calcium-dependent protein kinase 14 OS=Arabidopsis thaliana OX=3702 GN=CPK14 PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 4.9e-58
Identity = 116/272 (42.65%), Postives = 173/272 (63.60%), Query Frame = 0

Query: 6   VNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALL 65
           +  +Y++  ELGRG FG+   C    + + FACK+I K  L    D E + +E +IM  +
Sbjct: 50  IKQKYKLGRELGRGEFGVTYLCTEIETGEIFACKSILKKKLKTSIDIEDVKREVEIMRQM 109

Query: 66  PSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRR-PFSESEAAVVMKQLLQALAHC 125
           P HPNI+ L +  E+D  + L+ +LCE   L+DRI+ R  ++E  AA V+K +++ +  C
Sbjct: 110 PEHPNIVTLKETYEDDKAVHLVMELCEGGELFDRIVARGHYTERAAASVIKTIIEVVQMC 169

Query: 126 HSHGVVHRDVKPDNVLFDSRN---NLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRG 185
           H HGV+HRD+KP+N LF ++    +LK IDFG + +         +VG+PYY+APEVLR 
Sbjct: 170 HKHGVMHRDLKPENFLFANKKETASLKAIDFGLSVFFKPGERFNEIVGSPYYMAPEVLRR 229

Query: 186 SEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
           S YG+++D+WSAGVILY +L G PPF+ E+   V +A+L+  + F    +  VS  AKDL
Sbjct: 230 S-YGQEIDIWSAGVILYILLCGVPPFWAETEHGVAKAILKSVIDFKRDPWPKVSDNAKDL 289

Query: 246 MKKMICRDVSRRFSAEQALRHPWILSGGEATS 274
           +KKM+  D  RR +A+Q L HPWI +G  A++
Sbjct: 290 IKKMLHPDPRRRLTAQQVLDHPWIQNGKNASN 320

BLAST of CsGy1G032130 vs. ExPASy Swiss-Prot
Match: Q9C6P3 (Calcium-dependent protein kinase 33 OS=Arabidopsis thaliana OX=3702 GN=CPK33 PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 8.3e-58
Identity = 110/267 (41.20%), Postives = 175/267 (65.54%), Query Frame = 0

Query: 10  YQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALLPSHP 69
           Y + +ELGRG+FG+   C    + K FACK+ISK  L  + DKE + +E +IM  L   P
Sbjct: 73  YTLSKELGRGQFGVTYLCTEKSTGKRFACKSISKKKLVTKGDKEDMRREIQIMQHLSGQP 132

Query: 70  NILQLIDVLENDHYLCLISDLCESLSLYDRIIRR-PFSESEAAVVMKQLLQALAHCHSHG 129
           NI++     E++  + L+ +LC    L+DRI+ +  +SE  AA V +Q++  +  CH  G
Sbjct: 133 NIVEFKGAYEDEKAVNLVMELCAGGELFDRILAKGHYSERAAASVCRQIVNVVNICHFMG 192

Query: 130 VVHRDVKPDNVLFDSRNN---LKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRGSEYG 189
           V+HRD+KP+N L  S++    +K  DFG + + +E      +VG+ YYVAPEVL+   YG
Sbjct: 193 VMHRDLKPENFLLSSKDEKALIKATDFGLSVFIEEGRVYKDIVGSAYYVAPEVLK-RRYG 252

Query: 190 RKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKKM 249
           +++D+WSAG+ILY +L+G PPF+ E+   +F+A+L G + F ++ + S+S++AKDL+++M
Sbjct: 253 KEIDIWSAGIILYILLSGVPPFWAETEKGIFDAILEGEIDFESQPWPSISNSAKDLVRRM 312

Query: 250 ICRDVSRRFSAEQALRHPWILSGGEAT 273
           + +D  RR SA + L+HPW+  GGEA+
Sbjct: 313 LTQDPKRRISAAEVLKHPWLREGGEAS 338

BLAST of CsGy1G032130 vs. NCBI nr
Match: XP_011660276.2 (LOW QUALITY PROTEIN: phosphoenolpyruvate carboxylase kinase 1 [Cucumis sativus])

HSP 1 Score: 563 bits (1450), Expect = 2.15e-202
Identity = 275/276 (99.64%), Postives = 275/276 (99.64%), Query Frame = 0

Query: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           MADMIVNSQYQILEEL RGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK
Sbjct: 1   MADMIVNSQYQILEELXRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60

Query: 61  IMALLPSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLPSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA
Sbjct: 61  IMALLPSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 276
           LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 276

BLAST of CsGy1G032130 vs. NCBI nr
Match: XP_008462700.1 (PREDICTED: phosphoenolpyruvate carboxylase kinase 1 [Cucumis melo] >KAA0046511.1 phosphoenolpyruvate carboxylase kinase 1 [Cucumis melo var. makuwa] >TYK28749.1 phosphoenolpyruvate carboxylase kinase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 551 bits (1420), Expect = 8.05e-198
Identity = 269/276 (97.46%), Postives = 270/276 (97.83%), Query Frame = 0

Query: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           MADMIVNSQYQILEE+GRGRFGIITRCFCPISNKFFACKTISK DL DETDKECLVKEPK
Sbjct: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKTDLIDETDKECLVKEPK 60

Query: 61  IMALLPSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLP HPNILQLIDVLENDHYLCLISDLCE LSLYDRIIRRPFSESEAAVVMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCEPLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 276
           LMKKMICRDVSRR SAEQALRHPWILSGGE TSSVE
Sbjct: 241 LMKKMICRDVSRRLSAEQALRHPWILSGGEGTSSVE 276

BLAST of CsGy1G032130 vs. NCBI nr
Match: XP_038877083.1 (phosphoenolpyruvate carboxylase kinase 1-like [Benincasa hispida])

HSP 1 Score: 532 bits (1371), Expect = 2.46e-190
Identity = 257/277 (92.78%), Postives = 269/277 (97.11%), Query Frame = 0

Query: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           M DMIVNSQYQILEE+GRGRFG ITRCFCPISNKFFACKTI+KNDL DETDKECL KEPK
Sbjct: 1   MGDMIVNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPK 60

Query: 61  IMALLPSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMA+LP HPNILQLIDVLEND YLCLISDLC+SLSLYDRIIRRPFSESEAA+V+KQLL+A
Sbjct: 61  IMAILPPHPNILQLIDVLENDDYLCLISDLCDSLSLYDRIIRRPFSESEAAIVIKQLLEA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSA+WCDEDGFM+GVVGTPYY+APEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSADWCDEDGFMFGVVGTPYYIAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATS-SVE 276
           LMKKMICRDVSRRFSAEQ+LRHPWILSGGE T+ SVE
Sbjct: 241 LMKKMICRDVSRRFSAEQSLRHPWILSGGEGTATSVE 277

BLAST of CsGy1G032130 vs. NCBI nr
Match: XP_022988181.1 (phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita maxima])

HSP 1 Score: 523 bits (1347), Expect = 1.08e-186
Identity = 250/276 (90.58%), Postives = 265/276 (96.01%), Query Frame = 0

Query: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           M DMIVNSQY+ILEE+GRGRFG ITRCFCPISNKFFACKTI+K+DL DETDKECL KEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 61  IMALLPSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLP HPNILQLIDV END YLCLIS+LC+S+SLYDRIIRRPFSESEAA+VMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCH HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWCD+DGF +GVVGTPYY+APEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYG+KVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFP RVFRS+SSAAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 276
           LMKKMICRDVSRRFSAEQALRHPWILSGGE TSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEGTSSVE 276

BLAST of CsGy1G032130 vs. NCBI nr
Match: XP_022961225.1 (phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita moschata] >XP_022961226.1 phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita moschata])

HSP 1 Score: 520 bits (1340), Expect = 1.26e-185
Identity = 248/276 (89.86%), Postives = 264/276 (95.65%), Query Frame = 0

Query: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           M DMIVNSQY+ILEE+GRGRFG ITRCFCPISNKFFACKTI+K+DL DETDKECL KEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 61  IMALLPSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLP HPNILQLIDV END YLCLIS+LC+S+SLYDRIIRRPFSESEAA+VMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCH HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWCD+DGF +GVVGTPYY+APEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYG+KVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFP RVFRS+S AAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 276
           LMKKMICRDVSRRFSAEQALRHPWI+SGGE TSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 276

BLAST of CsGy1G032130 vs. ExPASy TrEMBL
Match: A0A0A0M3G7 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G690150 PE=3 SV=1)

HSP 1 Score: 565 bits (1457), Expect = 8.90e-204
Identity = 276/276 (100.00%), Postives = 276/276 (100.00%), Query Frame = 0

Query: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK
Sbjct: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60

Query: 61  IMALLPSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLPSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA
Sbjct: 61  IMALLPSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 276
           LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 276

BLAST of CsGy1G032130 vs. ExPASy TrEMBL
Match: A0A5A7TYV2 (Phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00970 PE=3 SV=1)

HSP 1 Score: 551 bits (1420), Expect = 3.90e-198
Identity = 269/276 (97.46%), Postives = 270/276 (97.83%), Query Frame = 0

Query: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           MADMIVNSQYQILEE+GRGRFGIITRCFCPISNKFFACKTISK DL DETDKECLVKEPK
Sbjct: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKTDLIDETDKECLVKEPK 60

Query: 61  IMALLPSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLP HPNILQLIDVLENDHYLCLISDLCE LSLYDRIIRRPFSESEAAVVMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCEPLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 276
           LMKKMICRDVSRR SAEQALRHPWILSGGE TSSVE
Sbjct: 241 LMKKMICRDVSRRLSAEQALRHPWILSGGEGTSSVE 276

BLAST of CsGy1G032130 vs. ExPASy TrEMBL
Match: A0A1S3CHK9 (phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo OX=3656 GN=LOC103501000 PE=3 SV=1)

HSP 1 Score: 551 bits (1420), Expect = 3.90e-198
Identity = 269/276 (97.46%), Postives = 270/276 (97.83%), Query Frame = 0

Query: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           MADMIVNSQYQILEE+GRGRFGIITRCFCPISNKFFACKTISK DL DETDKECLVKEPK
Sbjct: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKTDLIDETDKECLVKEPK 60

Query: 61  IMALLPSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLP HPNILQLIDVLENDHYLCLISDLCE LSLYDRIIRRPFSESEAAVVMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCEPLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 276
           LMKKMICRDVSRR SAEQALRHPWILSGGE TSSVE
Sbjct: 241 LMKKMICRDVSRRLSAEQALRHPWILSGGEGTSSVE 276

BLAST of CsGy1G032130 vs. ExPASy TrEMBL
Match: A0A6J1JGI4 (phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111485498 PE=3 SV=1)

HSP 1 Score: 523 bits (1347), Expect = 5.24e-187
Identity = 250/276 (90.58%), Postives = 265/276 (96.01%), Query Frame = 0

Query: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           M DMIVNSQY+ILEE+GRGRFG ITRCFCPISNKFFACKTI+K+DL DETDKECL KEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 61  IMALLPSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLP HPNILQLIDV END YLCLIS+LC+S+SLYDRIIRRPFSESEAA+VMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCH HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWCD+DGF +GVVGTPYY+APEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYG+KVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFP RVFRS+SSAAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 276
           LMKKMICRDVSRRFSAEQALRHPWILSGGE TSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEGTSSVE 276

BLAST of CsGy1G032130 vs. ExPASy TrEMBL
Match: A0A6J1HBL3 (phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461799 PE=3 SV=1)

HSP 1 Score: 520 bits (1340), Expect = 6.11e-186
Identity = 248/276 (89.86%), Postives = 264/276 (95.65%), Query Frame = 0

Query: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           M DMIVNSQY+ILEE+GRGRFG ITRCFCPISNKFFACKTI+K+DL DETDKECL KEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 61  IMALLPSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLP HPNILQLIDV END YLCLIS+LC+S+SLYDRIIRRPFSESEAA+VMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCH HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWCD+DGF +GVVGTPYY+APEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYG+KVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFP RVFRS+S AAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 276
           LMKKMICRDVSRRFSAEQALRHPWI+SGGE TSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 276

BLAST of CsGy1G032130 vs. TAIR 10
Match: AT1G08650.1 (phosphoenolpyruvate carboxylase kinase 1 )

HSP 1 Score: 340.1 bits (871), Expect = 1.7e-93
Identity = 161/266 (60.53%), Postives = 203/266 (76.32%), Query Frame = 0

Query: 8   SQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALLPS 67
           ++YQI EE+GRGRFG ++R + P +  FFACKTI K  L+D+ D+ CL  EPK+MALL  
Sbjct: 13  NKYQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLSDDLDRACLDNEPKLMALLSY 72

Query: 68  HPNILQLIDVLENDHYLCLISDLCE-SLSLYDRIIRR-PFSESEAAVVMKQLLQALAHCH 127
           HPNI+Q+ D+++ D  L +  +L   S+S+YDR++    F E + A   KQ+LQAL+HCH
Sbjct: 73  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 132

Query: 128 SHGVVHRDVKPDNVLFDSRNN-LKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRGSEY 187
            +GVVHRD+KP+N+L D RN+ +K+ DFGS  W  E     GVVGTPYYVAPEVL G  Y
Sbjct: 133 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEGETTEGVVGTPYYVAPEVLMGYSY 192

Query: 188 GRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKK 247
           G KVD+WSAGV+LYTMLAG PPFYGE+A E+FEAVLRGNLRFPT++FR VSS AKD ++K
Sbjct: 193 GEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLRK 252

Query: 248 MICRDVSRRFSAEQALRHPWILSGGE 271
           +IC+D SRRFSAEQALRHPWI   GE
Sbjct: 253 LICKDASRRFSAEQALRHPWIQRAGE 278

BLAST of CsGy1G032130 vs. TAIR 10
Match: AT1G08650.2 (phosphoenolpyruvate carboxylase kinase 1 )

HSP 1 Score: 322.8 bits (826), Expect = 2.7e-88
Identity = 154/256 (60.16%), Postives = 196/256 (76.56%), Query Frame = 0

Query: 8   SQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALLPS 67
           ++YQI EE+GRGRFG ++R + P +  FFACKTI K  L+D+ D+ CL  EPK+MALL  
Sbjct: 13  NKYQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLSDDLDRACLDNEPKLMALLSY 72

Query: 68  HPNILQLIDVLENDHYLCLISDLCE-SLSLYDRIIRR-PFSESEAAVVMKQLLQALAHCH 127
           HPNI+Q+ D+++ D  L +  +L   S+S+YDR++    F E + A   KQ+LQAL+HCH
Sbjct: 73  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 132

Query: 128 SHGVVHRDVKPDNVLFDSRNN-LKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRGSEY 187
            +GVVHRD+KP+N+L D RN+ +K+ DFGS  W  E     GVVGTPYYVAPEVL G  Y
Sbjct: 133 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEGETTEGVVGTPYYVAPEVLMGYSY 192

Query: 188 GRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKK 247
           G KVD+WSAGV+LYTMLAG PPFYGE+A E+FEAVLRGNLRFPT++FR VSS AKD ++K
Sbjct: 193 GEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLRK 252

Query: 248 MICRDVSRRFSAEQAL 261
           +IC+D SRRFSAEQAL
Sbjct: 253 LICKDASRRFSAEQAL 268

BLAST of CsGy1G032130 vs. TAIR 10
Match: AT3G04530.1 (phosphoenolpyruvate carboxylase kinase 2 )

HSP 1 Score: 320.5 bits (820), Expect = 1.4e-87
Identity = 156/273 (57.14%), Postives = 202/273 (73.99%), Query Frame = 0

Query: 6   VNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALL 65
           + + YQ+ +E+GRGRFG ITRCF P + +F+ACKTI K  L D  D+EC+  EP+IMA+L
Sbjct: 7   LENNYQLCDEIGRGRFGTITRCFSPATKEFYACKTIDKRVLIDALDRECIETEPRIMAML 66

Query: 66  PSHPNILQLIDVLENDHYLCLISDLCE-SLSLYDRIIRR--PFSESEAAVVMKQLLQALA 125
           P HPNI+++ D+ E +  L ++ +L +  +++YDR+I      SESE+A   KQ+L ALA
Sbjct: 67  PPHPNIIRIFDLYETEDSLAIVMELVDPPMTIYDRLISAGGRLSESESASYAKQILSALA 126

Query: 126 HCHSHGVVHRDVKPDNVLFD-SRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRG 185
           HCH   VVHRDVKPDNVL D     +KL DFGSA W   +    GVVGTPYYVAPEV+ G
Sbjct: 127 HCHRCDVVHRDVKPDNVLVDLVSGGVKLCDFGSAVWLGGE-TAEGVVGTPYYVAPEVVMG 186

Query: 186 SEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
            +Y  KVD+WSAGV++YTMLAG PPF GE+A ++FE++LRGNLRFP + F SVSS AKDL
Sbjct: 187 RKYDEKVDIWSAGVVIYTMLAGEPPFNGETAEDIFESILRGNLRFPPKKFGSVSSEAKDL 246

Query: 246 MKKMICRDVSRRFSAEQALRHPWILSGGEATSS 275
           ++KMICRDVSRRFSAE ALRH W+++ G   S+
Sbjct: 247 LRKMICRDVSRRFSAEDALRHSWMMNVGNLQSN 278

BLAST of CsGy1G032130 vs. TAIR 10
Match: AT2G41860.2 (calcium-dependent protein kinase 14 )

HSP 1 Score: 226.5 bits (576), Expect = 2.7e-59
Identity = 116/272 (42.65%), Postives = 173/272 (63.60%), Query Frame = 0

Query: 6   VNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALL 65
           +  +Y++  ELGRG FG+   C    + + FACK+I K  L    D E + +E +IM  +
Sbjct: 50  IKQKYKLGRELGRGEFGVTYLCTEIETGEIFACKSILKKKLKTSIDIEDVKREVEIMRQM 109

Query: 66  PSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRR-PFSESEAAVVMKQLLQALAHC 125
           P HPNI+ L +  E+D  + L+ +LCE   L+DRI+ R  ++E  AA V+K +++ +  C
Sbjct: 110 PEHPNIVTLKETYEDDKAVHLVMELCEGGELFDRIVARGHYTERAAASVIKTIIEVVQMC 169

Query: 126 HSHGVVHRDVKPDNVLFDSRN---NLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRG 185
           H HGV+HRD+KP+N LF ++    +LK IDFG + +         +VG+PYY+APEVLR 
Sbjct: 170 HKHGVMHRDLKPENFLFANKKETASLKAIDFGLSVFFKPGDRFNEIVGSPYYMAPEVLRR 229

Query: 186 SEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
           S YG+++D+WSAGVILY +L G PPF+ E+   V +A+L+  + F    +  VS  AKDL
Sbjct: 230 S-YGQEIDIWSAGVILYILLCGVPPFWAETEHGVAKAILKSVIDFKRDPWPKVSDNAKDL 289

Query: 246 MKKMICRDVSRRFSAEQALRHPWILSGGEATS 274
           +KKM+  D  RR +A+Q L HPWI +G  A++
Sbjct: 290 IKKMLHPDPRRRLTAQQVLDHPWIQNGKNASN 320

BLAST of CsGy1G032130 vs. TAIR 10
Match: AT2G41860.1 (calcium-dependent protein kinase 14 )

HSP 1 Score: 226.1 bits (575), Expect = 3.5e-59
Identity = 116/272 (42.65%), Postives = 173/272 (63.60%), Query Frame = 0

Query: 6   VNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALL 65
           +  +Y++  ELGRG FG+   C    + + FACK+I K  L    D E + +E +IM  +
Sbjct: 50  IKQKYKLGRELGRGEFGVTYLCTEIETGEIFACKSILKKKLKTSIDIEDVKREVEIMRQM 109

Query: 66  PSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRR-PFSESEAAVVMKQLLQALAHC 125
           P HPNI+ L +  E+D  + L+ +LCE   L+DRI+ R  ++E  AA V+K +++ +  C
Sbjct: 110 PEHPNIVTLKETYEDDKAVHLVMELCEGGELFDRIVARGHYTERAAASVIKTIIEVVQMC 169

Query: 126 HSHGVVHRDVKPDNVLFDSRN---NLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRG 185
           H HGV+HRD+KP+N LF ++    +LK IDFG + +         +VG+PYY+APEVLR 
Sbjct: 170 HKHGVMHRDLKPENFLFANKKETASLKAIDFGLSVFFKPGERFNEIVGSPYYMAPEVLRR 229

Query: 186 SEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
           S YG+++D+WSAGVILY +L G PPF+ E+   V +A+L+  + F    +  VS  AKDL
Sbjct: 230 S-YGQEIDIWSAGVILYILLCGVPPFWAETEHGVAKAILKSVIDFKRDPWPKVSDNAKDL 289

Query: 246 MKKMICRDVSRRFSAEQALRHPWILSGGEATS 274
           +KKM+  D  RR +A+Q L HPWI +G  A++
Sbjct: 290 IKKMLHPDPRRRLTAQQVLDHPWIQNGKNASN 320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SPK42.3e-9260.53Phosphoenolpyruvate carboxylase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PPCK... [more]
Q93VK01.9e-8657.14Phosphoenolpyruvate carboxylase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PPCK... [more]
P285823.7e-5841.70Calcium-dependent protein kinase OS=Daucus carota OX=4039 PE=2 SV=2[more]
P937594.9e-5842.65Calcium-dependent protein kinase 14 OS=Arabidopsis thaliana OX=3702 GN=CPK14 PE=... [more]
Q9C6P38.3e-5841.20Calcium-dependent protein kinase 33 OS=Arabidopsis thaliana OX=3702 GN=CPK33 PE=... [more]
Match NameE-valueIdentityDescription
XP_011660276.22.15e-20299.64LOW QUALITY PROTEIN: phosphoenolpyruvate carboxylase kinase 1 [Cucumis sativus][more]
XP_008462700.18.05e-19897.46PREDICTED: phosphoenolpyruvate carboxylase kinase 1 [Cucumis melo] >KAA0046511.1... [more]
XP_038877083.12.46e-19092.78phosphoenolpyruvate carboxylase kinase 1-like [Benincasa hispida][more]
XP_022988181.11.08e-18690.58phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita maxima][more]
XP_022961225.11.26e-18589.86phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita moschata] >XP_022961226... [more]
Match NameE-valueIdentityDescription
A0A0A0M3G78.90e-204100.00Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G690... [more]
A0A5A7TYV23.90e-19897.46Phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3CHK93.90e-19897.46phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo OX=3656 GN=LOC103501000... [more]
A0A6J1JGI45.24e-18790.58phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1HBL36.11e-18689.86phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
AT1G08650.11.7e-9360.53phosphoenolpyruvate carboxylase kinase 1 [more]
AT1G08650.22.7e-8860.16phosphoenolpyruvate carboxylase kinase 1 [more]
AT3G04530.11.4e-8757.14phosphoenolpyruvate carboxylase kinase 2 [more]
AT2G41860.22.7e-5942.65calcium-dependent protein kinase 14 [more]
AT2G41860.13.5e-5942.65calcium-dependent protein kinase 14 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 10..265
e-value: 5.5E-93
score: 324.9
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 10..265
e-value: 4.4E-72
score: 242.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 10..265
score: 48.088829
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 3..275
e-value: 2.6E-88
score: 298.4
NoneNo IPR availablePANTHERPTHR24349SERINE/THREONINE-PROTEIN KINASEcoord: 6..274
NoneNo IPR availablePANTHERPTHR24349:SF410PHOSPHOENOLPYRUVATE CARBOXYLASE KINASE 1coord: 6..274
NoneNo IPR availableCDDcd05117STKc_CAMKcoord: 9..264
e-value: 2.23728E-125
score: 355.631
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 129..141
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 16..39
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 4..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy1G032130.2CsGy1G032130.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0018105 peptidyl-serine phosphorylation
biological_process GO:0046777 protein autophosphorylation
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0009931 calcium-dependent protein serine/threonine kinase activity
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0004683 calmodulin-dependent protein kinase activity
molecular_function GO:0004672 protein kinase activity