CsGy1G027570 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy1G027570
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionmitogen-activated protein kinase kinase 2
LocationGy14Chr1: 26002413 .. 26007043 (-)
RNA-Seq ExpressionCsGy1G027570
SyntenyCsGy1G027570
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGGTATTAGGTTCATAGATGAAATTGAACACTAACAATTTAAAAAGTTAGAAATTTGATATGGTATGAATGTAGTTGAGGCATATTTAAATTGGATTTTGCAATATTGGGAGTTTGAAAAGTTAGAATTTTGTAAGAAAAATCAAAGGAAAAAGAAAAGTCAAAGATTCAAAAGAAGAAAAGTAAACGGTCCCGCATCCTCCAATCCAATCCAACCAAGAACAGCCCTATTTTTCTTTTTCTTTTCAATGTTTAAATCAGCTCTTAGTGTCACAAAAATGGTGTAATTAGGGTTTTTGAGTTTGGATTTTTTGTAAATTCTATCAGTTTCATTCTCAACTACTGCTCAACACTTGATTAATTCATATATATAAACAAATTCTTGACATTTCTCTGCAATTATCTCTCTCTTTCTCTCTCTACATCGCAAAAGGCTTCAATCCGAGTATCAATTCTACACGGTGGTAGTCTGGAAGAGATCAGCCATCAATGAGGAAAGGAGGCTTCAGCAACAACCTCAATCTCAAGCTCAATCTTCCTAAAGAAGACCAATCCATAGCGACGTTCCTGTAATCTTTCTCTCTGTGTGCTTGTGCTTGTGTGTGTATGTGTGTAAGTGAAAATTTCTGATGAATCTGGAATCTGGGTTTGTTTTTCTTGTGTAGAACGCAGAGTGGCACGTTTAAAGATGGTGATCTGCTTGTAAACAGAGATGGGGTTCGGATTGTTTCTCAAAGCGAAGTTGAAGCAGTAAGAGTTCTTAGTTTTGTAGCTTAGTGTGACTTTAGAGGACTATAATGTGCTGTAGTTTTCAATGTTATGGCTGAGGGAATTTGGATTTTAGCTCATTAACTTTTCAAGTATGGGTTTTCTTGCTACGTATTAAGTATTTGTTTGGTTTCTGTTGAGTTGCTTAAAGTATTCTGCAGTGAAGTATGGAATTACAAATCCGTATACTATGCTTGATGTACTGTGCTCACTTCACTATGGGTTTCATTTGTGATTGGGTTTCATTTGTTACTAACTCCTAGTTAACTAAATCTCTGTGTGATATCGTTTTCATTTTTAGGTTCTATTTCTTAAAATTATGCTTGTTGCTTTATCATTTCTTTACTATGGTTTTCATTTCTCTTTAAGAATCATTTAGATTCAAAGCCAAATTCCAAATAAACAAAATCACAATCTACATTTTTTTTATTTAGTTTTCAAAAACTTGGGTTTGATAACTTTTCAAGGGAGTAGATGACAAAATAAAGGAACTCATAAGTGGAAATAGTGTTTGTAATCTTTATTTTTTAAATTTAAGAATTAGGTGGTTATCATTGAAATTCAGACTAATTGATTGAGGTATATAATACGTGAGATTCCTATACTGAACTTGTTTGTTTTTCAACACATAGGGAACAAAGTGAAAAAGTGGTTGATTATGTTGTCTTGTGAACTATCTTGTGTATATTAAGCTACTAACTTGTTTTCCTTCTACCTATGTGTTCAGCCACCTCCGATTAAGCCTACAGAGGATCAGTTGAGTTTAGCGGACATAGACATTATTAAAGTCATTGGAAAAGGAAATGGTGGTACTGTGCAATTAGTTCAGCACAAATGGACTGCTCAGTTTTTTGCATTGAAGGTACTGCACAACTTAAACATTATTGAAATTCTCTGTCTCTGACTTCTATGATTTATATACTTTATAGTTGAGGCTTCAAAAAGTGGTTAGAGCATCTTAATCATCATTATATTGAAGGTATATTTTTCTTGTGAACTGAAAGATGGTTAATAATAAATAAAAAGAAAAGAAGGTATATTTTTCTTTCACCAGCAGTTTCACATTCCTATTCGTACACTACCCATTAGAAATCTTAGAATCAAGAAAAAAAGTAGTAACTATCTGCATTAATTAGTAAAAAAAATATTTCAAACCATATCAAATACCAAATCATTTCGTCTACTCTTTCAGGTAATTCAGATGAAAATTGAAGAGTCTCACCGCAAGCAGATTGCACAAGAACTGAAAATCAATCAATCAGCGCAGTGCCCTTATGTTGTTGTCTGTTACCAGTCTTTCTATGATAATGGATCAATATATATCATCCTAGAGTACATGGATGGAGGATCTTTAGCAGATTTTTTAAAAAAGGTTAAAAAAATTGAAGAACCATATCTTGCTGCCCTTTGTAAGCAGGTTGGTACAATAAGTCTTTGCTTGGATTTGGAGAATTCTTTTTTAATTAATTTATTTTTCAATTTTAGGAGATAATATAAGATTTCCTTTTTAAACCCCTTTCTCCTTCCATACAGGTACTGAAGGGGTTGTCTTACCTTCACCATGAAAGACACATCATCCATAGGGACTTGAAGCCTTCAAATTTATTAATAAACCATAGAGGGGAAGTCAAGATTACTGACTTTGGTGTGAGTGCAATTATGGAAAACACATATGAAGAGGCTAATACTTTTGTTGGCACGTATAACTATATGTCCGTGAGTATCAATTCTCAATATATTCGGATCAATACAATTCTCGGGCTGTCTTTCCTTTATTTTTTCTGGTAAAGCTGTACGTTTTTCCTTCCATACCTATGTGTATAACTGACTGGTGTCTCTATGTATTGTCGTTTATAGCCAGAGAGAATTGTGGGAGAGGGATATGACAATAAAAGTGACATTTGGAGCTTGGGTCTGATATTACTTGAGTGTGCAACCGGAAAGTTTCCTTATTCTCCACCCGGGCAAGATGGAGGATGGGTTAATTTTTATGAGCTTATGGAAGCCATTGTTGAAGGCGAACCTCCTTCTGCTCCGGCTGACCAATTTACTCCTGAATTCTGTTCATTTATTTCTGCATGGTAAGGACCCAACTTTTCCTATAGTCCCTTTTCCTGAGATTATGACAATCATTATATGTACTTCATGTAGAAATTTCTAAATGTAACTGTAAAGTAGGTATTTTCTTATACTCTAAGCTTATTTCAAGTGTTTTTACATTATGAGATTTCTGACTCAGAAACCTGTTATCTTTATGAATATCAGTGTGCAAACAGACCCAAAGAATAGACTGTCAGCACGTGAACTTTTGGTATATAATTCTTCCTTTCTACATATTGATAAGCAAGATGTCAACACAATAGAAAAAATAAATCTGAATTTTGTCTTCACTTTTTGTGTGACTTTCAGGAACATCCTTTCATCAAGATGTATGAAGATAAGGATATTGATCTATCATCTTACTTCAATGATGCAGGATCTCCACTTGCAACTTTCTAAATTTTATCTGGTAAGTTAGGAACTACATCCCTTTTAAGTTTACCTTTCAAAAAAATAAGAAAGAAAGAAAGAAAAAACTACGTCTCTTTTAAAGCTTCCTCTTGATATCTTTTTTAATTCTGTTTTGAATTACGTGATTGAGGTGATCATTTCTTGCAACCGAAGCTATCATTCAGTGTAATGAAGTGGAAATTTCTGTCTTCATTATTTGCATGCTAAACATATATTTTAAACTATGTTTTCCCCTTATAGCTCGTTTATAAATTTTTATTTTATTTTTAACCAACTGTTTATCTCCTTTGGTACTTTGAGATTCTTCTGCTTTCAGCTTTTTGCCAGTTCCCATCATAGCTTTGTATGACTGTGTGCATTTTTTATATGATTAAATTAAGTACGCCAGCTTTCTTTGGGACAATAGACTTAAAGTTAGCTGCATTTGACTGTGTATAGCAAGATGAAGATCAAACTTCTGACCTTTTAGGCCATATAAGAGCACACAAGGACAAGCGGCTTAGATGAAACCTGTTTCAAACTTATATGGTAGGTTAATTTAAAAAATCAGATCTACAGAAAGTCGCTAGAAATGGAATCTATGTATATAGTATGGTGATATTTCTGACTATCACTTTACTTGAATGATGAAATATAGAAATGTTAGGTAAAAATATTATGCTATGGTTAATGGCTCCAACATTCATGGCCAATTGAAATACCTAGAACTAATGAATGTTGGAATTTTTTGATATTGTTTCATGTCCTTCTGTCTCTGTTTCTCTCATTCAAGCTTTTACCCATTAACTGGTGTTTATGCTGATAATGCATCATTTTCTCGACAGTTGATCGTCTCTGGCTCGTACACTGGAATCTTATTTGTTTGCTGCTTGCTGCAAGGATGGACGAGGAGTCCGCTGGGATCTCGGTTTCTCATGAATGCTGCTTGTTTTACTTCAGTTTGTGTCTTAATGAAAGAGGTTTCAATCAAAACTGGATGATATAGTTGTTCCTATGCTGGTCACTAATTTGGATGATTCTGATCCTTCTTGTGGATGGCTCAGAATGCTAAACGGGATATGATGTTGTGTAAAGAGATTTCAAATCATGGCCATTTCTTGTAACATTTTGTAATCCAATTCCACCGCTACTCATGTAAAATTTGAAGCATCAAAGGAAAATAAATACATATTGTTATCTCAAGTTTCTGTCTTGTATTGTTATCTCAAGTTAAATTTGCTCGTGTAGCTTCTAAACATTATTAGGATGGCCATAGCCATCCACAAGAGAAAGAACACAAGTACGCAGTATAAGAGAATTTGGATTGCACAAAATAAACAAAATTCACTTTCAGCTTATTTGAAAGGAACGGA

mRNA sequence

GAGGTATTAGGTTCATAGATGAAATTGAACACTAACAATTTAAAAAGTTAGAAATTTGATATGGTATGAATGTAGTTGAGGCATATTTAAATTGGATTTTGCAATATTGGGAGTTTGAAAAGTTAGAATTTTGTAAGAAAAATCAAAGGAAAAAGAAAAGTCAAAGATTCAAAAGAAGAAAAGTAAACGGTCCCGCATCCTCCAATCCAATCCAACCAAGAACAGCCCTATTTTTCTTTTTCTTTTCAATGTTTAAATCAGCTCTTAGTGTCACAAAAATGGTGTAATTAGGGTTTTTGAGTTTGGATTTTTTGTAAATTCTATCAGTTTCATTCTCAACTACTGCTCAACACTTGATTAATTCATATATATAAACAAATTCTTGACATTTCTCTGCAATTATCTCTCTCTTTCTCTCTCTACATCGCAAAAGGCTTCAATCCGAGTATCAATTCTACACGGTGGTAGTCTGGAAGAGATCAGCCATCAATGAGGAAAGGAGGCTTCAGCAACAACCTCAATCTCAAGCTCAATCTTCCTAAAGAAGACCAATCCATAGCGACGTTCCTAACGCAGAGTGGCACGTTTAAAGATGGTGATCTGCTTGTAAACAGAGATGGGGTTCGGATTGTTTCTCAAAGCGAAGTTGAAGCACCACCTCCGATTAAGCCTACAGAGGATCAGTTGAGTTTAGCGGACATAGACATTATTAAAGTCATTGGAAAAGGAAATGGTGGTACTGTGCAATTAGTTCAGCACAAATGGACTGCTCAGTTTTTTGCATTGAAGGTAATTCAGATGAAAATTGAAGAGTCTCACCGCAAGCAGATTGCACAAGAACTGAAAATCAATCAATCAGCGCAGTGCCCTTATGTTGTTGTCTGTTACCAGTCTTTCTATGATAATGGATCAATATATATCATCCTAGAGTACATGGATGGAGGATCTTTAGCAGATTTTTTAAAAAAGGTTAAAAAAATTGAAGAACCATATCTTGCTGCCCTTTGTAAGCAGGTACTGAAGGGGTTGTCTTACCTTCACCATGAAAGACACATCATCCATAGGGACTTGAAGCCTTCAAATTTATTAATAAACCATAGAGGGGAAGTCAAGATTACTGACTTTGGTGTGAGTGCAATTATGGAAAACACATATGAAGAGGCTAATACTTTTGTTGGCACGTATAACTATATGTCCCCAGAGAGAATTGTGGGAGAGGGATATGACAATAAAAGTGACATTTGGAGCTTGGGTCTGATATTACTTGAGTGTGCAACCGGAAAGTTTCCTTATTCTCCACCCGGGCAAGATGGAGGATGGGTTAATTTTTATGAGCTTATGGAAGCCATTGTTGAAGGCGAACCTCCTTCTGCTCCGGCTGACCAATTTACTCCTGAATTCTGTTCATTTATTTCTGCATGTGTGCAAACAGACCCAAAGAATAGACTGTCAGCACGTGAACTTTTGGAACATCCTTTCATCAAGATGTATGAAGATAAGGATATTGATCTATCATCTTACTTCAATGATGCAGGATCTCCACTTGCAACTTTCTAAATTTTATCTGTTGATCGTCTCTGGCTCGTACACTGGAATCTTATTTGTTTGCTGCTTGCTGCAAGGATGGACGAGGAGTCCGCTGGGATCTCGGTTTCTCATGAATGCTGCTTGTTTTACTTCAGTTTGTGTCTTAATGAAAGAGGTTTCAATCAAAACTGGATGATATAGTTGTTCCTATGCTGGTCACTAATTTGGATGATTCTGATCCTTCTTGTGGATGGCTCAGAATGCTAAACGGGATATGATGTTGTGTAAAGAGATTTCAAATCATGGCCATTTCTTGTAACATTTTGTAATCCAATTCCACCGCTACTCATGTAAAATTTGAAGCATCAAAGGAAAATAAATACATATTGTTATCTCAAGTTTCTGTCTTGTATTGTTATCTCAAGTTAAATTTGCTCGTGTAGCTTCTAAACATTATTAGGATGGCCATAGCCATCCACAAGAGAAAGAACACAAGTACGCAGTATAAGAGAATTTGGATTGCACAAAATAAACAAAATTCACTTTCAGCTTATTTGAAAGGAACGGA

Coding sequence (CDS)

ATGAGGAAAGGAGGCTTCAGCAACAACCTCAATCTCAAGCTCAATCTTCCTAAAGAAGACCAATCCATAGCGACGTTCCTAACGCAGAGTGGCACGTTTAAAGATGGTGATCTGCTTGTAAACAGAGATGGGGTTCGGATTGTTTCTCAAAGCGAAGTTGAAGCACCACCTCCGATTAAGCCTACAGAGGATCAGTTGAGTTTAGCGGACATAGACATTATTAAAGTCATTGGAAAAGGAAATGGTGGTACTGTGCAATTAGTTCAGCACAAATGGACTGCTCAGTTTTTTGCATTGAAGGTAATTCAGATGAAAATTGAAGAGTCTCACCGCAAGCAGATTGCACAAGAACTGAAAATCAATCAATCAGCGCAGTGCCCTTATGTTGTTGTCTGTTACCAGTCTTTCTATGATAATGGATCAATATATATCATCCTAGAGTACATGGATGGAGGATCTTTAGCAGATTTTTTAAAAAAGGTTAAAAAAATTGAAGAACCATATCTTGCTGCCCTTTGTAAGCAGGTACTGAAGGGGTTGTCTTACCTTCACCATGAAAGACACATCATCCATAGGGACTTGAAGCCTTCAAATTTATTAATAAACCATAGAGGGGAAGTCAAGATTACTGACTTTGGTGTGAGTGCAATTATGGAAAACACATATGAAGAGGCTAATACTTTTGTTGGCACGTATAACTATATGTCCCCAGAGAGAATTGTGGGAGAGGGATATGACAATAAAAGTGACATTTGGAGCTTGGGTCTGATATTACTTGAGTGTGCAACCGGAAAGTTTCCTTATTCTCCACCCGGGCAAGATGGAGGATGGGTTAATTTTTATGAGCTTATGGAAGCCATTGTTGAAGGCGAACCTCCTTCTGCTCCGGCTGACCAATTTACTCCTGAATTCTGTTCATTTATTTCTGCATGTGTGCAAACAGACCCAAAGAATAGACTGTCAGCACGTGAACTTTTGGAACATCCTTTCATCAAGATGTATGAAGATAAGGATATTGATCTATCATCTTACTTCAATGATGCAGGATCTCCACTTGCAACTTTCTAA

Protein sequence

MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTEDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKINQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGLSYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERIVGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQFTPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF*
Homology
BLAST of CsGy1G027570 vs. ExPASy Swiss-Prot
Match: Q9S7U9 (Mitogen-activated protein kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=MKK2 PE=1 SV=2)

HSP 1 Score: 518.5 bits (1334), Expect = 6.1e-146
Identity = 250/354 (70.62%), Postives = 293/354 (82.77%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           M+KGGFSNNL L + +  E QSI  FLTQSGTFKDGDL VN+DGVRI+SQ E E   PIK
Sbjct: 1   MKKGGFSNNLKLAIPVAGE-QSITKFLTQSGTFKDGDLRVNKDGVRIISQLEPEVLSPIK 60

Query: 61  PTEDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           P +DQLSL+D+D++KVIGKG+ G VQLVQHKWT QFFALKVIQ+ I+E+ RK IAQELKI
Sbjct: 61  PADDQLSLSDLDMVKVIGKGSSGVVQLVQHKWTGQFFALKVIQLNIDEAIRKAIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQS+QCP +V  YQSFYDNG+I +ILEYMDGGSLADFLK VK I + YL+A+ +QVL+GL
Sbjct: 121 NQSSQCPNLVTSYQSFYDNGAISLILEYMDGGSLADFLKSVKAIPDSYLSAIFRQVLQGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
            YLHH+RHIIHRDLKPSNLLINHRGEVKITDFGVS +M NT   ANTFVGTYNYMSPERI
Sbjct: 181 IYLHHDRHIIHRDLKPSNLLINHRGEVKITDFGVSTVMTNTAGLANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           VG  Y NKSDIWSLGL++LECATGKFPY+PP Q+  W + +ELMEAIV+  PP+ P+  F
Sbjct: 241 VGNKYGNKSDIWSLGLVVLECATGKFPYAPPNQEETWTSVFELMEAIVDQPPPALPSGNF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLAT 355
           +PE  SFIS C+Q DP +R SA+EL+EHPF+  Y+   I+L+SYF DAGSPLAT
Sbjct: 301 SPELSSFISTCLQKDPNSRSSAKELMEHPFLNKYDYSGINLASYFTDAGSPLAT 353

BLAST of CsGy1G027570 vs. ExPASy Swiss-Prot
Match: Q94A06 (Mitogen-activated protein kinase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=MKK1 PE=1 SV=2)

HSP 1 Score: 488.0 bits (1255), Expect = 8.9e-137
Identity = 236/341 (69.21%), Postives = 281/341 (82.40%), Query Frame = 0

Query: 16  LPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTEDQLSLADIDIIK 75
           LP  +QSI+ FLTQSGTFKDGDL VN+DG++ VS SE  APPPI+P ++QLSLAD+++IK
Sbjct: 13  LPPLEQSISKFLTQSGTFKDGDLRVNKDGIQTVSLSEPGAPPPIEPLDNQLSLADLEVIK 72

Query: 76  VIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKINQSAQCPYVVVCYQS 135
           VIGKG+ G VQLV+HK T QFFALKVIQ+  EES  + I+QEL+IN S+QCPY+V CYQS
Sbjct: 73  VIGKGSSGNVQLVKHKLTQQFFALKVIQLNTEESTCRAISQELRINLSSQCPYLVSCYQS 132

Query: 136 FYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGLSYLHHERHIIHRDLK 195
           FY NG + IILE+MDGGSLAD LKKV K+ E  L+A+CK+VL+GL Y+HHER IIHRDLK
Sbjct: 133 FYHNGLVSIILEFMDGGSLADLLKKVGKVPENMLSAICKRVLRGLCYIHHERRIIHRDLK 192

Query: 196 PSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERIVGEGYDNKSDIWSLG 255
           PSNLLINHRGEVKITDFGVS I+ +T   AN+FVGTY YMSPERI G  Y NKSDIWSLG
Sbjct: 193 PSNLLINHRGEVKITDFGVSKILTSTSSLANSFVGTYPYMSPERISGSLYSNKSDIWSLG 252

Query: 256 LILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQFTPEFCSFISACVQTD 315
           L+LLECATGKFPY+PP    GW + YEL++AIVE  PP AP++ F+PEFCSFIS CVQ D
Sbjct: 253 LVLLECATGKFPYTPPEHKKGWSSVYELVDAIVENPPPCAPSNLFSPEFCSFISQCVQKD 312

Query: 316 PKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGS---PLA 354
           P++R SA+ELLEH F+KM+ED D +LS+YF DAGS   PLA
Sbjct: 313 PRDRKSAKELLEHKFVKMFEDSDTNLSAYFTDAGSLIPPLA 353

BLAST of CsGy1G027570 vs. ExPASy Swiss-Prot
Match: Q5QN75 (Mitogen-activated protein kinase kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MKK1 PE=1 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 5.0e-124
Identity = 207/332 (62.35%), Postives = 269/332 (81.02%), Query Frame = 0

Query: 12  LKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPP--IKPTEDQLSLA 71
           LKL++P ++  +  FLT SGTFKDG+L +N+ G++++S+   + P    +K  + QLS+ 
Sbjct: 10  LKLSVPAQETPVDKFLTASGTFKDGELRLNQRGLQLISEETADEPQSTNLKVEDVQLSMD 69

Query: 72  DIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKINQSAQCPYV 131
           D+++I+VIGKG+GG VQLV+HKW    +ALK IQM I+E+ RKQI QELKINQ+ Q  ++
Sbjct: 70  DLEMIQVIGKGSGGIVQLVRHKWVGTLYALKGIQMNIQEAVRKQIVQELKINQATQNAHI 129

Query: 132 VVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGLSYLHHERHI 191
           V+C+QSFY NG IY++LEYMD GSLAD +K+VK I EPYLA LCKQVL+GL YLHHERH+
Sbjct: 130 VLCHQSFYHNGVIYLVLEYMDRGSLADIIKQVKTILEPYLAVLCKQVLEGLLYLHHERHV 189

Query: 192 IHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERIVGEGYDNKS 251
           IHRD+KPSNLL+N +GEVKITDFGVSA++ ++  + +TFVGTYNYM+PERI G  YD KS
Sbjct: 190 IHRDIKPSNLLVNRKGEVKITDFGVSAVLASSMGQRDTFVGTYNYMAPERISGSSYDYKS 249

Query: 252 DIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQFTPEFCSFIS 311
           DIWSLGL++LECA G+FPY  P +  GW++FYEL+EAIV+  PPSAPADQF+PEFC+FIS
Sbjct: 250 DIWSLGLVILECAIGRFPYI-PSEGEGWLSFYELLEAIVDQPPPSAPADQFSPEFCAFIS 309

Query: 312 ACVQTDPKNRLSARELLEHPFIKMYEDKDIDL 342
           +C+Q DP  R+SA ELL HPFIK +EDKD+DL
Sbjct: 310 SCIQKDPAERMSASELLNHPFIKKFEDKDLDL 340

BLAST of CsGy1G027570 vs. ExPASy Swiss-Prot
Match: A9RWC9 (Mitogen-activated protein kinase kinase 1a OS=Physcomitrium patens OX=3218 GN=MKK1a PE=2 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 6.8e-121
Identity = 204/342 (59.65%), Postives = 269/342 (78.65%), Query Frame = 0

Query: 3   KGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPT 62
           KGG   N  L++N+PKE+ +I+ ++T SGTF+DGD+++N++G+R+VSQ++   P      
Sbjct: 9   KGG---NSALRVNVPKEN-AISDYVTASGTFQDGDIMLNKEGIRVVSQTQTSTP----AA 68

Query: 63  EDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKINQ 122
              +SL+D++ +KV+GKG+GG VQLV+HKWT + +ALKVI M IEE+ RKQI QELKIN 
Sbjct: 69  NGNISLSDLETVKVLGKGSGGVVQLVRHKWTNETYALKVIHMNIEETTRKQIVQELKINH 128

Query: 123 SAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKV--KKIEEPYLAALCKQVLKGL 182
           ++QCPYVV+CY +FY+NG I I+LEYMDGGSLAD +K++  K I+EP L  +CKQVL GL
Sbjct: 129 ASQCPYVVICYHAFYNNGLISIVLEYMDGGSLADIMKELEPKCIKEPNLGVVCKQVLLGL 188

Query: 183 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 242
            YLH  RHIIHRD+KPSNLL+NH+GEVKI+DFGVSA++ N+    +TFVGT  YMSPER+
Sbjct: 189 MYLHQTRHIIHRDIKPSNLLVNHKGEVKISDFGVSAVLANSMAVRDTFVGTCTYMSPERV 248

Query: 243 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 302
           +G  Y   SDIWSLGL LLECA GK+PY PPG + GW+NFYEL++ IV+  PP APADQF
Sbjct: 249 LGGTYGFDSDIWSLGLTLLECALGKYPYQPPGSEEGWMNFYELLQTIVDQPPPVAPADQF 308

Query: 303 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLS 343
           +PEFCSFISAC+Q DPK R +A ELL HPF+  Y++++  L+
Sbjct: 309 SPEFCSFISACLQKDPKCRPTAAELLNHPFVNKYDEEEYGLA 342

BLAST of CsGy1G027570 vs. ExPASy Swiss-Prot
Match: Q9FJV0 (Mitogen-activated protein kinase kinase 6 OS=Arabidopsis thaliana OX=3702 GN=MKK6 PE=1 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 3.7e-119
Identity = 203/336 (60.42%), Postives = 259/336 (77.08%), Query Frame = 0

Query: 7   SNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTEDQL 66
           SN   LKL++P ++  I++FLT SGTF DGD L+N+ G+R+ S  +       K  + ++
Sbjct: 6   SNLKQLKLSVPAQESPISSFLTASGTFHDGDFLLNQKGLRLTSDEKQSRQSDSKELDFEI 65

Query: 67  SLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKINQ-SAQ 126
           +  D++ +KVIGKG+GG VQLV+HKW  +FFA+KVIQM I+E  RKQI QELKINQ S+Q
Sbjct: 66  TAEDLETVKVIGKGSGGVVQLVRHKWVGKFFAMKVIQMNIQEEIRKQIVQELKINQASSQ 125

Query: 127 CPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGLSYLHH 186
           CP+VVVCY SFY NG+  ++LEYMD GSLAD +++VK I EPYLA +CKQVL GL YLH+
Sbjct: 126 CPHVVVCYHSFYHNGAFSLVLEYMDRGSLADVIRQVKTILEPYLAVVCKQVLLGLVYLHN 185

Query: 187 ERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERIVGEGY 246
           ERH+IHRD+KPSNLL+NH+GEVKI+DFGVSA + ++  + +TFVGTYNYMSPERI G  Y
Sbjct: 186 ERHVIHRDIKPSNLLVNHKGEVKISDFGVSASLASSMGQRDTFVGTYNYMSPERISGSTY 245

Query: 247 DNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQFTPEFC 306
           D  SDIWSLG+ +LECA G+FPY          +FYEL+ AIVE  PP+AP+DQF+PEFC
Sbjct: 246 DYSSDIWSLGMSVLECAIGRFPYLESEDQQNPPSFYELLAAIVENPPPTAPSDQFSPEFC 305

Query: 307 SFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDL 342
           SF+SAC+Q DP  R S+ +LL HPFIK +EDKDIDL
Sbjct: 306 SFVSACIQKDPPARASSLDLLSHPFIKKFEDKDIDL 341

BLAST of CsGy1G027570 vs. NCBI nr
Match: XP_004144014.1 (mitogen-activated protein kinase kinase 2 [Cucumis sativus] >KGN66268.1 hypothetical protein Csa_006896 [Cucumis sativus])

HSP 1 Score: 723 bits (1867), Expect = 2.70e-263
Identity = 354/355 (99.72%), Postives = 355/355 (100.00%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK
Sbjct: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60

Query: 61  PTEDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           PT+DQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI
Sbjct: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL
Sbjct: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
           SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI
Sbjct: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF
Sbjct: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355
           TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF
Sbjct: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355

BLAST of CsGy1G027570 vs. NCBI nr
Match: XP_008450884.2 (PREDICTED: LOW QUALITY PROTEIN: mitogen-activated protein kinase kinase 2-like [Cucumis melo])

HSP 1 Score: 712 bits (1839), Expect = 5.02e-259
Identity = 347/355 (97.75%), Postives = 353/355 (99.44%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           MRKGGFSNNLNLKLNLPKE+QSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK
Sbjct: 1   MRKGGFSNNLNLKLNLPKEEQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60

Query: 61  PTEDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           PT+DQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI
Sbjct: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQSAQCPY+VVCYQSFYDNGSIYIILEYMDGGSLADFLKKVK I+EPYLAALCKQVLKGL
Sbjct: 121 NQSAQCPYIVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKXIQEPYLAALCKQVLKGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
           SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTY+EANTFVGTYNYMSPERI
Sbjct: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYQEANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQD GWVNFYELMEAIVEGEPPSAPADQF
Sbjct: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDEGWVNFYELMEAIVEGEPPSAPADQF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355
           TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYED+DIDLSSYFNDAGSPLATF
Sbjct: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDRDIDLSSYFNDAGSPLATF 355

BLAST of CsGy1G027570 vs. NCBI nr
Match: XP_038880098.1 (mitogen-activated protein kinase kinase 2-like [Benincasa hispida] >XP_038880099.1 mitogen-activated protein kinase kinase 2-like [Benincasa hispida])

HSP 1 Score: 699 bits (1804), Expect = 1.09e-253
Identity = 343/355 (96.62%), Postives = 348/355 (98.03%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK
Sbjct: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60

Query: 61  PTEDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           PT+DQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI
Sbjct: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQSAQCPY+VVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKI+EPYLAALCKQVLKGL
Sbjct: 121 NQSAQCPYIVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIQEPYLAALCKQVLKGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
           SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIM +T  EANTFVGTYNYMSPERI
Sbjct: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMASTSGEANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           VGE YDNKSDIWSLGLILLECATGKFPYSPP QDGGWVNFYELMEAIVEGEPPSAPADQF
Sbjct: 241 VGERYDNKSDIWSLGLILLECATGKFPYSPPEQDGGWVNFYELMEAIVEGEPPSAPADQF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355
           TP FCSFISACVQTDPK+RLSARELLEHPFIKMYED DIDLSSYFNDAGSPLATF
Sbjct: 301 TPNFCSFISACVQTDPKDRLSARELLEHPFIKMYEDTDIDLSSYFNDAGSPLATF 355

BLAST of CsGy1G027570 vs. NCBI nr
Match: XP_023516502.1 (mitogen-activated protein kinase kinase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 688 bits (1776), Expect = 2.01e-249
Identity = 334/355 (94.08%), Postives = 346/355 (97.46%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           MRKGGFSNNLNLKLNLP+EDQSIA FLTQSGTFKDGDLLVNRDGVRIVS+SEVEAPPPIK
Sbjct: 1   MRKGGFSNNLNLKLNLPQEDQSIAKFLTQSGTFKDGDLLVNRDGVRIVSKSEVEAPPPIK 60

Query: 61  PTEDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           PT+DQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI
Sbjct: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQSAQCPY+VVCYQSFYDNGSIYIILEYMDGGSLADFLKKVK+++EPYLAALCKQVLKGL
Sbjct: 121 NQSAQCPYIVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKQVQEPYLAALCKQVLKGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
           SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIM +TY EANTFVGTYNYMSPERI
Sbjct: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMASTYGEANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           +GE YDNKSDIWSLGLI LECATGKFPYSPP Q+GGWVNFYELMEAIVEGEPPSAPADQF
Sbjct: 241 IGEKYDNKSDIWSLGLIFLECATGKFPYSPPEQEGGWVNFYELMEAIVEGEPPSAPADQF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355
           TP FCSFISACVQ DP++RLSARELLEHPFIKMYED DIDLSSYFNDAGSPLATF
Sbjct: 301 TPNFCSFISACVQKDPRDRLSARELLEHPFIKMYEDTDIDLSSYFNDAGSPLATF 355

BLAST of CsGy1G027570 vs. NCBI nr
Match: XP_022961151.1 (mitogen-activated protein kinase kinase 2 [Cucurbita moschata] >KAG7023678.1 Mitogen-activated protein kinase kinase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 687 bits (1773), Expect = 5.77e-249
Identity = 333/355 (93.80%), Postives = 346/355 (97.46%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           MRKGGFSNNLNLKLNLP++DQSIA FLTQSGTFKDGDLLVNRDGVRIVS+SEVEAPPPIK
Sbjct: 1   MRKGGFSNNLNLKLNLPQDDQSIAKFLTQSGTFKDGDLLVNRDGVRIVSKSEVEAPPPIK 60

Query: 61  PTEDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           PT+DQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI
Sbjct: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQSAQCPY+VVCYQSFYDNGSIYIILEYMDGGSLADFLKKVK+++EPYLAALCKQVLKGL
Sbjct: 121 NQSAQCPYIVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKQVQEPYLAALCKQVLKGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
           SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIM +TY EANTFVGTYNYMSPERI
Sbjct: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMASTYGEANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           +GE YDNKSDIWSLGLI LECATGKFPYSPP Q+GGWVNFYELMEAIVEGEPPSAPADQF
Sbjct: 241 IGEKYDNKSDIWSLGLIFLECATGKFPYSPPEQEGGWVNFYELMEAIVEGEPPSAPADQF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355
           TP FCSFISACVQ DP++RLSARELLEHPFIKMYED DIDLSSYFNDAGSPLATF
Sbjct: 301 TPNFCSFISACVQKDPRDRLSARELLEHPFIKMYEDTDIDLSSYFNDAGSPLATF 355

BLAST of CsGy1G027570 vs. ExPASy TrEMBL
Match: A0A0A0LWW3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G589750 PE=3 SV=1)

HSP 1 Score: 723 bits (1867), Expect = 1.31e-263
Identity = 354/355 (99.72%), Postives = 355/355 (100.00%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK
Sbjct: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60

Query: 61  PTEDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           PT+DQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI
Sbjct: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL
Sbjct: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
           SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI
Sbjct: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF
Sbjct: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355
           TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF
Sbjct: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355

BLAST of CsGy1G027570 vs. ExPASy TrEMBL
Match: A0A1S3BQA4 (LOW QUALITY PROTEIN: mitogen-activated protein kinase kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103492345 PE=3 SV=1)

HSP 1 Score: 712 bits (1839), Expect = 2.43e-259
Identity = 347/355 (97.75%), Postives = 353/355 (99.44%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           MRKGGFSNNLNLKLNLPKE+QSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK
Sbjct: 1   MRKGGFSNNLNLKLNLPKEEQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60

Query: 61  PTEDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           PT+DQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI
Sbjct: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQSAQCPY+VVCYQSFYDNGSIYIILEYMDGGSLADFLKKVK I+EPYLAALCKQVLKGL
Sbjct: 121 NQSAQCPYIVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKXIQEPYLAALCKQVLKGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
           SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTY+EANTFVGTYNYMSPERI
Sbjct: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYQEANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQD GWVNFYELMEAIVEGEPPSAPADQF
Sbjct: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDEGWVNFYELMEAIVEGEPPSAPADQF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355
           TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYED+DIDLSSYFNDAGSPLATF
Sbjct: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDRDIDLSSYFNDAGSPLATF 355

BLAST of CsGy1G027570 vs. ExPASy TrEMBL
Match: A0A6J1H9J6 (mitogen-activated protein kinase kinase 2 OS=Cucurbita moschata OX=3662 GN=LOC111461750 PE=3 SV=1)

HSP 1 Score: 687 bits (1773), Expect = 2.79e-249
Identity = 333/355 (93.80%), Postives = 346/355 (97.46%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           MRKGGFSNNLNLKLNLP++DQSIA FLTQSGTFKDGDLLVNRDGVRIVS+SEVEAPPPIK
Sbjct: 1   MRKGGFSNNLNLKLNLPQDDQSIAKFLTQSGTFKDGDLLVNRDGVRIVSKSEVEAPPPIK 60

Query: 61  PTEDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           PT+DQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI
Sbjct: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQSAQCPY+VVCYQSFYDNGSIYIILEYMDGGSLADFLKKVK+++EPYLAALCKQVLKGL
Sbjct: 121 NQSAQCPYIVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKQVQEPYLAALCKQVLKGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
           SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIM +TY EANTFVGTYNYMSPERI
Sbjct: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMASTYGEANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           +GE YDNKSDIWSLGLI LECATGKFPYSPP Q+GGWVNFYELMEAIVEGEPPSAPADQF
Sbjct: 241 IGEKYDNKSDIWSLGLIFLECATGKFPYSPPEQEGGWVNFYELMEAIVEGEPPSAPADQF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355
           TP FCSFISACVQ DP++RLSARELLEHPFIKMYED DIDLSSYFNDAGSPLATF
Sbjct: 301 TPNFCSFISACVQKDPRDRLSARELLEHPFIKMYEDTDIDLSSYFNDAGSPLATF 355

BLAST of CsGy1G027570 vs. ExPASy TrEMBL
Match: A0A6J1JJS8 (mitogen-activated protein kinase kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485219 PE=3 SV=1)

HSP 1 Score: 686 bits (1770), Expect = 8.01e-249
Identity = 332/355 (93.52%), Postives = 346/355 (97.46%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           MRKGGFSNNLNLKLNLP++DQSIA FLTQSGTFKDGDLLVNRDGVRIVS+SEVEAPPPIK
Sbjct: 1   MRKGGFSNNLNLKLNLPQDDQSIAKFLTQSGTFKDGDLLVNRDGVRIVSKSEVEAPPPIK 60

Query: 61  PTEDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           PT+DQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI
Sbjct: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQSAQCPY+VVCYQSFYDNGSIYIILEYMDGGSLADFLKKVK+++EPYLAALCKQVLKGL
Sbjct: 121 NQSAQCPYIVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKQVQEPYLAALCKQVLKGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
           SYLHH+RHIIHRDLKPSNLLINHRGEVKITDFGVSAIM +TY EANTFVGTYNYMSPERI
Sbjct: 181 SYLHHDRHIIHRDLKPSNLLINHRGEVKITDFGVSAIMASTYGEANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           +GE YDNKSDIWSLGLI LECATGKFPYSPP Q+GGWVNFYELMEAIVEGEPPSAPADQF
Sbjct: 241 IGEKYDNKSDIWSLGLIFLECATGKFPYSPPEQEGGWVNFYELMEAIVEGEPPSAPADQF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355
           TP FCSFISACVQ DP++RLSARELLEHPFIKMYED DIDLSSYFNDAGSPLATF
Sbjct: 301 TPNFCSFISACVQKDPRDRLSARELLEHPFIKMYEDTDIDLSSYFNDAGSPLATF 355

BLAST of CsGy1G027570 vs. ExPASy TrEMBL
Match: A0A6J1D344 (mitogen-activated protein kinase kinase 2-like OS=Momordica charantia OX=3673 GN=LOC111016574 PE=3 SV=1)

HSP 1 Score: 669 bits (1727), Expect = 2.66e-242
Identity = 329/355 (92.68%), Postives = 339/355 (95.49%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           MRKGGFS NL  KLNLP+EDQSIA FLTQSGTF+DGDLLVNRDGVRIVSQSEVEAPPPIK
Sbjct: 1   MRKGGFSTNL--KLNLPQEDQSIAKFLTQSGTFRDGDLLVNRDGVRIVSQSEVEAPPPIK 60

Query: 61  PTEDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           PT+DQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI
Sbjct: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQSAQCPY+V+CYQSFYDNGSIYIILEYMDGGSLADFLKKVKKI+EPYLAA+CKQVLKGL
Sbjct: 121 NQSAQCPYIVICYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIQEPYLAAICKQVLKGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
            YLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIM +T  EANTFVGTYNYMSPERI
Sbjct: 181 LYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMASTSGEANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           VG  YDNKSDIW LGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAP+DQF
Sbjct: 241 VGARYDNKSDIWCLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPSDQF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355
           TP FC+FISACVQ DPK RLSARELLEHPFIKMYED  IDLSSYFNDAGSPLATF
Sbjct: 301 TPNFCAFISACVQQDPKVRLSARELLEHPFIKMYEDTAIDLSSYFNDAGSPLATF 353

BLAST of CsGy1G027570 vs. TAIR 10
Match: AT4G29810.1 (MAP kinase kinase 2 )

HSP 1 Score: 518.5 bits (1334), Expect = 4.4e-147
Identity = 250/354 (70.62%), Postives = 293/354 (82.77%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           M+KGGFSNNL L + +  E QSI  FLTQSGTFKDGDL VN+DGVRI+SQ E E   PIK
Sbjct: 1   MKKGGFSNNLKLAIPVAGE-QSITKFLTQSGTFKDGDLRVNKDGVRIISQLEPEVLSPIK 60

Query: 61  PTEDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           P +DQLSL+D+D++KVIGKG+ G VQLVQHKWT QFFALKVIQ+ I+E+ RK IAQELKI
Sbjct: 61  PADDQLSLSDLDMVKVIGKGSSGVVQLVQHKWTGQFFALKVIQLNIDEAIRKAIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQS+QCP +V  YQSFYDNG+I +ILEYMDGGSLADFLK VK I + YL+A+ +QVL+GL
Sbjct: 121 NQSSQCPNLVTSYQSFYDNGAISLILEYMDGGSLADFLKSVKAIPDSYLSAIFRQVLQGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
            YLHH+RHIIHRDLKPSNLLINHRGEVKITDFGVS +M NT   ANTFVGTYNYMSPERI
Sbjct: 181 IYLHHDRHIIHRDLKPSNLLINHRGEVKITDFGVSTVMTNTAGLANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           VG  Y NKSDIWSLGL++LECATGKFPY+PP Q+  W + +ELMEAIV+  PP+ P+  F
Sbjct: 241 VGNKYGNKSDIWSLGLVVLECATGKFPYAPPNQEETWTSVFELMEAIVDQPPPALPSGNF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLAT 355
           +PE  SFIS C+Q DP +R SA+EL+EHPF+  Y+   I+L+SYF DAGSPLAT
Sbjct: 301 SPELSSFISTCLQKDPNSRSSAKELMEHPFLNKYDYSGINLASYFTDAGSPLAT 353

BLAST of CsGy1G027570 vs. TAIR 10
Match: AT4G29810.2 (MAP kinase kinase 2 )

HSP 1 Score: 510.8 bits (1314), Expect = 9.1e-145
Identity = 250/363 (68.87%), Postives = 293/363 (80.72%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFL---------TQSGTFKDGDLLVNRDGVRIVSQS 60
           M+KGGFSNNL L + +  E QSI  FL         TQSGTFKDGDL VN+DGVRI+SQ 
Sbjct: 1   MKKGGFSNNLKLAIPVAGE-QSITKFLRKGFGSLCRTQSGTFKDGDLRVNKDGVRIISQL 60

Query: 61  EVEAPPPIKPTEDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHR 120
           E E   PIKP +DQLSL+D+D++KVIGKG+ G VQLVQHKWT QFFALKVIQ+ I+E+ R
Sbjct: 61  EPEVLSPIKPADDQLSLSDLDMVKVIGKGSSGVVQLVQHKWTGQFFALKVIQLNIDEAIR 120

Query: 121 KQIAQELKINQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAA 180
           K IAQELKINQS+QCP +V  YQSFYDNG+I +ILEYMDGGSLADFLK VK I + YL+A
Sbjct: 121 KAIAQELKINQSSQCPNLVTSYQSFYDNGAISLILEYMDGGSLADFLKSVKAIPDSYLSA 180

Query: 181 LCKQVLKGLSYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGT 240
           + +QVL+GL YLHH+RHIIHRDLKPSNLLINHRGEVKITDFGVS +M NT   ANTFVGT
Sbjct: 181 IFRQVLQGLIYLHHDRHIIHRDLKPSNLLINHRGEVKITDFGVSTVMTNTAGLANTFVGT 240

Query: 241 YNYMSPERIVGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGE 300
           YNYMSPERIVG  Y NKSDIWSLGL++LECATGKFPY+PP Q+  W + +ELMEAIV+  
Sbjct: 241 YNYMSPERIVGNKYGNKSDIWSLGLVVLECATGKFPYAPPNQEETWTSVFELMEAIVDQP 300

Query: 301 PPSAPADQFTPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSP 355
           PP+ P+  F+PE  SFIS C+Q DP +R SA+EL+EHPF+  Y+   I+L+SYF DAGSP
Sbjct: 301 PPALPSGNFSPELSSFISTCLQKDPNSRSSAKELMEHPFLNKYDYSGINLASYFTDAGSP 360

BLAST of CsGy1G027570 vs. TAIR 10
Match: AT4G26070.2 (MAP kinase/ ERK kinase 1 )

HSP 1 Score: 488.0 bits (1255), Expect = 6.3e-138
Identity = 236/341 (69.21%), Postives = 281/341 (82.40%), Query Frame = 0

Query: 16  LPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTEDQLSLADIDIIK 75
           LP  +QSI+ FLTQSGTFKDGDL VN+DG++ VS SE  APPPI+P ++QLSLAD+++IK
Sbjct: 13  LPPLEQSISKFLTQSGTFKDGDLRVNKDGIQTVSLSEPGAPPPIEPLDNQLSLADLEVIK 72

Query: 76  VIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKINQSAQCPYVVVCYQS 135
           VIGKG+ G VQLV+HK T QFFALKVIQ+  EES  + I+QEL+IN S+QCPY+V CYQS
Sbjct: 73  VIGKGSSGNVQLVKHKLTQQFFALKVIQLNTEESTCRAISQELRINLSSQCPYLVSCYQS 132

Query: 136 FYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGLSYLHHERHIIHRDLK 195
           FY NG + IILE+MDGGSLAD LKKV K+ E  L+A+CK+VL+GL Y+HHER IIHRDLK
Sbjct: 133 FYHNGLVSIILEFMDGGSLADLLKKVGKVPENMLSAICKRVLRGLCYIHHERRIIHRDLK 192

Query: 196 PSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERIVGEGYDNKSDIWSLG 255
           PSNLLINHRGEVKITDFGVS I+ +T   AN+FVGTY YMSPERI G  Y NKSDIWSLG
Sbjct: 193 PSNLLINHRGEVKITDFGVSKILTSTSSLANSFVGTYPYMSPERISGSLYSNKSDIWSLG 252

Query: 256 LILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQFTPEFCSFISACVQTD 315
           L+LLECATGKFPY+PP    GW + YEL++AIVE  PP AP++ F+PEFCSFIS CVQ D
Sbjct: 253 LVLLECATGKFPYTPPEHKKGWSSVYELVDAIVENPPPCAPSNLFSPEFCSFISQCVQKD 312

Query: 316 PKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGS---PLA 354
           P++R SA+ELLEH F+KM+ED D +LS+YF DAGS   PLA
Sbjct: 313 PRDRKSAKELLEHKFVKMFEDSDTNLSAYFTDAGSLIPPLA 353

BLAST of CsGy1G027570 vs. TAIR 10
Match: AT4G26070.3 (MAP kinase/ ERK kinase 1 )

HSP 1 Score: 488.0 bits (1255), Expect = 6.3e-138
Identity = 236/341 (69.21%), Postives = 281/341 (82.40%), Query Frame = 0

Query: 16  LPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTEDQLSLADIDIIK 75
           LP  +QSI+ FLTQSGTFKDGDL VN+DG++ VS SE  APPPI+P ++QLSLAD+++IK
Sbjct: 13  LPPLEQSISKFLTQSGTFKDGDLRVNKDGIQTVSLSEPGAPPPIEPLDNQLSLADLEVIK 72

Query: 76  VIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKINQSAQCPYVVVCYQS 135
           VIGKG+ G VQLV+HK T QFFALKVIQ+  EES  + I+QEL+IN S+QCPY+V CYQS
Sbjct: 73  VIGKGSSGNVQLVKHKLTQQFFALKVIQLNTEESTCRAISQELRINLSSQCPYLVSCYQS 132

Query: 136 FYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGLSYLHHERHIIHRDLK 195
           FY NG + IILE+MDGGSLAD LKKV K+ E  L+A+CK+VL+GL Y+HHER IIHRDLK
Sbjct: 133 FYHNGLVSIILEFMDGGSLADLLKKVGKVPENMLSAICKRVLRGLCYIHHERRIIHRDLK 192

Query: 196 PSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERIVGEGYDNKSDIWSLG 255
           PSNLLINHRGEVKITDFGVS I+ +T   AN+FVGTY YMSPERI G  Y NKSDIWSLG
Sbjct: 193 PSNLLINHRGEVKITDFGVSKILTSTSSLANSFVGTYPYMSPERISGSLYSNKSDIWSLG 252

Query: 256 LILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQFTPEFCSFISACVQTD 315
           L+LLECATGKFPY+PP    GW + YEL++AIVE  PP AP++ F+PEFCSFIS CVQ D
Sbjct: 253 LVLLECATGKFPYTPPEHKKGWSSVYELVDAIVENPPPCAPSNLFSPEFCSFISQCVQKD 312

Query: 316 PKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGS---PLA 354
           P++R SA+ELLEH F+KM+ED D +LS+YF DAGS   PLA
Sbjct: 313 PRDRKSAKELLEHKFVKMFEDSDTNLSAYFTDAGSLIPPLA 353

BLAST of CsGy1G027570 vs. TAIR 10
Match: AT4G29810.3 (MAP kinase kinase 2 )

HSP 1 Score: 471.1 bits (1211), Expect = 8.0e-133
Identity = 232/335 (69.25%), Postives = 271/335 (80.90%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFL---------TQSGTFKDGDLLVNRDGVRIVSQS 60
           M+KGGFSNNL L + +  E QSI  FL         TQSGTFKDGDL VN+DGVRI+SQ 
Sbjct: 1   MKKGGFSNNLKLAIPVAGE-QSITKFLRKGFGSLCRTQSGTFKDGDLRVNKDGVRIISQL 60

Query: 61  EVEAPPPIKPTEDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHR 120
           E E   PIKP +DQLSL+D+D++KVIGKG+ G VQLVQHKWT QFFALKVIQ+ I+E+ R
Sbjct: 61  EPEVLSPIKPADDQLSLSDLDMVKVIGKGSSGVVQLVQHKWTGQFFALKVIQLNIDEAIR 120

Query: 121 KQIAQELKINQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAA 180
           K IAQELKINQS+QCP +V  YQSFYDNG+I +ILEYMDGGSLADFLK VK I + YL+A
Sbjct: 121 KAIAQELKINQSSQCPNLVTSYQSFYDNGAISLILEYMDGGSLADFLKSVKAIPDSYLSA 180

Query: 181 LCKQVLKGLSYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGT 240
           + +QVL+GL YLHH+RHIIHRDLKPSNLLINHRGEVKITDFGVS +M NT   ANTFVGT
Sbjct: 181 IFRQVLQGLIYLHHDRHIIHRDLKPSNLLINHRGEVKITDFGVSTVMTNTAGLANTFVGT 240

Query: 241 YNYMSPERIVGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGE 300
           YNYMSPERIVG  Y NKSDIWSLGL++LECATGKFPY+PP Q+  W + +ELMEAIV+  
Sbjct: 241 YNYMSPERIVGNKYGNKSDIWSLGLVVLECATGKFPYAPPNQEETWTSVFELMEAIVDQP 300

Query: 301 PPSAPADQFTPEFCSFISACVQTDPKNRLSARELL 327
           PP+ P+  F+PE  SFIS C+Q DP +R SA+EL+
Sbjct: 301 PPALPSGNFSPELSSFISTCLQKDPNSRSSAKELM 334

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9S7U96.1e-14670.62Mitogen-activated protein kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Q94A068.9e-13769.21Mitogen-activated protein kinase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Q5QN755.0e-12462.35Mitogen-activated protein kinase kinase 1 OS=Oryza sativa subsp. japonica OX=399... [more]
A9RWC96.8e-12159.65Mitogen-activated protein kinase kinase 1a OS=Physcomitrium patens OX=3218 GN=MK... [more]
Q9FJV03.7e-11960.42Mitogen-activated protein kinase kinase 6 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Match NameE-valueIdentityDescription
XP_004144014.12.70e-26399.72mitogen-activated protein kinase kinase 2 [Cucumis sativus] >KGN66268.1 hypothet... [more]
XP_008450884.25.02e-25997.75PREDICTED: LOW QUALITY PROTEIN: mitogen-activated protein kinase kinase 2-like [... [more]
XP_038880098.11.09e-25396.62mitogen-activated protein kinase kinase 2-like [Benincasa hispida] >XP_038880099... [more]
XP_023516502.12.01e-24994.08mitogen-activated protein kinase kinase 2-like [Cucurbita pepo subsp. pepo][more]
XP_022961151.15.77e-24993.80mitogen-activated protein kinase kinase 2 [Cucurbita moschata] >KAG7023678.1 Mit... [more]
Match NameE-valueIdentityDescription
A0A0A0LWW31.31e-26399.72Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G589... [more]
A0A1S3BQA42.43e-25997.75LOW QUALITY PROTEIN: mitogen-activated protein kinase kinase 2-like OS=Cucumis m... [more]
A0A6J1H9J62.79e-24993.80mitogen-activated protein kinase kinase 2 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1JJS88.01e-24993.52mitogen-activated protein kinase kinase 2-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1D3442.66e-24292.68mitogen-activated protein kinase kinase 2-like OS=Momordica charantia OX=3673 GN... [more]
Match NameE-valueIdentityDescription
AT4G29810.14.4e-14770.62MAP kinase kinase 2 [more]
AT4G29810.29.1e-14568.87MAP kinase kinase 2 [more]
AT4G26070.26.3e-13869.21MAP kinase/ ERK kinase 1 [more]
AT4G26070.36.3e-13869.21MAP kinase/ ERK kinase 1 [more]
AT4G29810.38.0e-13369.25MAP kinase kinase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPRINTSPR00109TYRKINASEcoord: 146..159
score: 49.12
coord: 183..201
score: 35.52
coord: 249..271
score: 34.02
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 71..331
e-value: 8.3E-91
score: 317.7
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 73..331
e-value: 2.9E-72
score: 243.2
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 71..331
score: 49.657703
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 46..150
e-value: 6.4E-29
score: 102.1
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 151..351
e-value: 4.4E-65
score: 221.7
NoneNo IPR availablePIRSRPIRSR037014-1PIRSR037014-1coord: 71..271
e-value: 1.4E-29
score: 100.8
NoneNo IPR availablePIRSRPIRSR000605-51PIRSR000605-51coord: 59..269
e-value: 4.7E-34
score: 115.8
NoneNo IPR availablePANTHERPTHR24361:SF759MAP KINASE KINASEcoord: 9..354
NoneNo IPR availablePANTHERPTHR24361MITOGEN-ACTIVATED KINASE KINASE KINASEcoord: 9..354
NoneNo IPR availableCDDcd06623PKc_MAPKK_plant_likecoord: 69..335
e-value: 1.41855E-158
score: 443.187
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 189..201
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 77..100
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 55..336

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy1G027570.2CsGy1G027570.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity