CsGy1G025560 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy1G025560
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionAB hydrolase-1 domain-containing protein
LocationGy14Chr1: 24452511 .. 24455324 (+)
RNA-Seq ExpressionCsGy1G025560
SyntenyCsGy1G025560
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GATATTCCAAAATTTCCATATGAATTTGTAGAAAGGTGTGAAGCAAAAGGTTACTAAAGTTATACTTTTTTTCTTGAAAGGTTAGTTTTCCATAAAAAGACGAAAAAGTTAAAAACACAAATCAAATCCCCAAAAGAAAGACTTCGTTTGTCAGCAATCATAAAAAGATTAAGCAGAAAGATGAGAACTTCAAGAAACCCATTTATTGCTGAATACCCATTTAGTGCTTTTCATCTCCTCGGCCAAATTCCTGTGTTCAACCACCTCCCCTTCATTCAATTTCGTTTGCCGCTCCATTTTAAACCATACCCCATTTCTCTGCAACTGATAGTAATACGGAGAGAAACTAAAATTAACCCATCGCCATGGATACTATCAGCCACAGAACTGTGAGCGTTAATGGCATAAACATGCACATAGCTGAGAAGGGCGAAGGCCCAATTGTTCTTTTCATCCATGGTTTCCCAGAGCTTTGGTATACATGGCGCCATCAGATTTTGGCCTTGAGCTCTCTTGGGTACCACGCTGTGGCGCCGGACCTTCGTGGGTATGGCGATTCTGATGCCCCTGATTCCATTTCTAGCTATTCTATTATGCATATTGTTGGGGATCTTGTTGCTCTTGTTGAGAGTTTTGGGGTTAAGGAAGTTTTCGTTGTGGCGCATGATTGGGGCGCTTTGATTGCATGGTCTTTGTGTTTATTCCGAGCTGAGATGGTTAAGGCGTTTGTGTGTCTAAGTGTGCCGTTTAGGCCTAGGCATCCGAACAGGAAGCCTGTTGAGACCATGAGGATGGTTTTTGGAGATGATTACTACATCTGCAGATTCCAGGTTGGGTTCTTTGTTTTTTTTTTTTCTTTTTTTCTTTTTTTGAATGTTGAAATGAAAATCTTAGGCTTCGAGAGTGCTTTTCCCATGGTAATAATTAGTTTTTGAGTTTTTTTCCTAGTTGTCAAATAGTTGAATGGTGAAAATAAATTAAAACAGATGAACAGTGAGTTGAATAACGTAATTGAATTGATTCTCGGGTGAGAGTTACAAGATCTCATACCTTGAAATTCATGGCTATGTTACCGAACTAATTAGCATGGAATATTTTCTCTTGACCATCTTAATCTATGTTTGAAACTGATGTTGGCATGAGTTTGGGATGACTCTTGGAAACAGGATCAAATGGTTTCAACCAGTACAAATTTGCTTTTCCAACCTTTTCATACTGTTTGGTTGAAGAACGGAAAGTGGTTTCGAAATGTGTACGAATTACTTGGAACCTAACTTTTTGAAGTAATTATAGTTGAAGTATTTAATTAGCTACTCTGAAAAGTCAACAGAACACCACACCAAAGTGCATTTATGAGAAAGCACTTATAGCCCAAGTACGAGGCTAAAAGCACTTCTTTATTGTAACTATAAACTTGAAATCTTGAATACAAGGTCCTTAAGAAATATTGAACAGTGATTTATGTACTGGTTTTCTGGGATTAAAATAGCCTCTACTTAGGAGATTGTTTCCCTTTTTGTGCTTTTTTCTTTGAAAGAGAGTAATCAAACATAAGATTTGTTTTTCTTTTTTCTCCCTTTTAGAATCCAGGAGAGATTGAAGAAGAGATGGCTCAAGTGGGTGCCAAAGACGTACTAAGAGGCATATTAACAACAAGAAGACAAGGGCCTCCCATTTATCCCAAAAAACAAGCCTTCCGAGCCAGGGCCGGACCCCCATCTCCCTTGCCCTCTTGGCTTTCAGAAGAAGATCTTTCTTACTTTGCCTCCAAATATGAGCAGAAGGGATTCACTGGACCATTGAACTATTACAGATCAATGGACTTGTAAGTCACAAAACAAACTACAAACTTGCTGATTTTTAAAACCAAGAGCCAGCTCTTATGTCTAAACATTGCATTTTTTGTCCATACATCTTCATTATTTTAAAATTTTCATTATTGTCTTTTACCTTTACATCAAAGCTCACTCTGGGTAACTCTACTATTCTTCAAATTGGCATTCAGGTCTTTAACTTCTATCCATAAAACAAGATGTCACGTCATGAAATTTATCTGCATTCTTCTTGCTTTTTATTTTTCTACAATTCATCTAGGACCTCGTTGGCAAGACTGGTCTAATAAATGATAGAAACATCTAAAAGTATAGGGACCTAATTGTCACGGAATGCTAAGGATACGGACTGTATGTATGTATAACATAACCTATACTCGTTTTGGAAGTAAAAGACTGATTTGTATTTGGATTTTGGTGTAGAAACTGGGAGTTAACAGCACCATGGACAGGGGTTCAAGTGAAAGTGCCAGTGAAGTTCATAGTTGGAGATGTTGACATGGTCTATACAACACCAGGAGTTAAGGAGTATGTCAATGGTGGCGGTTTCAAGAAAGATGTGCCATTTCTGCAAGACGTTGTGGTAATGGATGGAGTTGGCCACTTCCTAAACCAAGAGAAGCCTGAAGAGATTAACACTCACATCTATGACTTCATCAAAAAATTCTAGACTGATTCAACAAGGATCATAGAAAAGTCTTTACTTTTCCAAAATACATGGCTGTCTTTTTCTTTTTCAGTTTTACGTTGTTTGATGCTTCTTTCAAGTGATTAGTATGTTTGAAAATCCAAGTGGTTGTTCTCCATGGGAGATACTTTTTCAATGTGTTAAAAATTTCCAATAACTTAAAAAACCAAGCGATCCAACCAACTCGGACTGTTTGGTTTGGTTATTGGCTCATTTGGGTTGGACCTTTCAAATAAATGAAATGTTAATTTGATTCAATCAAAACCTATAATTCTTAATTTTGATTTTTAAAAG

mRNA sequence

GATATTCCAAAATTTCCATATGAATTTGTAGAAAGGTGTGAAGCAAAAGGTTACTAAAGTTATACTTTTTTTCTTGAAAGGTTAGTTTTCCATAAAAAGACGAAAAAGTTAAAAACACAAATCAAATCCCCAAAAGAAAGACTTCGTTTGTCAGCAATCATAAAAAGATTAAGCAGAAAGATGAGAACTTCAAGAAACCCATTTATTGCTGAATACCCATTTAGTGCTTTTCATCTCCTCGGCCAAATTCCTGTGTTCAACCACCTCCCCTTCATTCAATTTCGTTTGCCGCTCCATTTTAAACCATACCCCATTTCTCTGCAACTGATAGTAATACGGAGAGAAACTAAAATTAACCCATCGCCATGGATACTATCAGCCACAGAACTGTGAGCGTTAATGGCATAAACATGCACATAGCTGAGAAGGGCGAAGGCCCAATTGTTCTTTTCATCCATGGTTTCCCAGAGCTTTGGTATACATGGCGCCATCAGATTTTGGCCTTGAGCTCTCTTGGGTACCACGCTGTGGCGCCGGACCTTCGTGGGTATGGCGATTCTGATGCCCCTGATTCCATTTCTAGCTATTCTATTATGCATATTGTTGGGGATCTTGTTGCTCTTGTTGAGAGTTTTGGGGTTAAGGAAGTTTTCGTTGTGGCGCATGATTGGGGCGCTTTGATTGCATGGTCTTTGTGTTTATTCCGAGCTGAGATGGTTAAGGCGTTTGTGTGTCTAAGTGTGCCGTTTAGGCCTAGGCATCCGAACAGGAAGCCTGTTGAGACCATGAGGATGGTTTTTGGAGATGATTACTACATCTGCAGATTCCAGAATCCAGGAGAGATTGAAGAAGAGATGGCTCAAGTGGGTGCCAAAGACGTACTAAGAGGCATATTAACAACAAGAAGACAAGGGCCTCCCATTTATCCCAAAAAACAAGCCTTCCGAGCCAGGGCCGGACCCCCATCTCCCTTGCCCTCTTGGCTTTCAGAAGAAGATCTTTCTTACTTTGCCTCCAAATATGAGCAGAAGGGATTCACTGGACCATTGAACTATTACAGATCAATGGACTTAAACTGGGAGTTAACAGCACCATGGACAGGGGTTCAAGTGAAAGTGCCAGTGAAGTTCATAGTTGGAGATGTTGACATGGTCTATACAACACCAGGAGTTAAGGAGTATGTCAATGGTGGCGGTTTCAAGAAAGATGTGCCATTTCTGCAAGACGTTGTGGTAATGGATGGAGTTGGCCACTTCCTAAACCAAGAGAAGCCTGAAGAGATTAACACTCACATCTATGACTTCATCAAAAAATTCTAGACTGATTCAACAAGGATCATAGAAAAGTCTTTACTTTTCCAAAATACATGGCTGTCTTTTTCTTTTTCAGTTTTACGTTGTTTGATGCTTCTTTCAAGTGATTAGTATGTTTGAAAATCCAAGTGGTTGTTCTCCATGGGAGATACTTTTTCAATGTGTTAAAAATTTCCAATAACTTAAAAAACCAAGCGATCCAACCAACTCGGACTGTTTGGTTTGGTTATTGGCTCATTTGGGTTGGACCTTTCAAATAAATGAAATGTTAATTTGATTCAATCAAAACCTATAATTCTTAATTTTGATTTTTAAAAG

Coding sequence (CDS)

ATGGATACTATCAGCCACAGAACTGTGAGCGTTAATGGCATAAACATGCACATAGCTGAGAAGGGCGAAGGCCCAATTGTTCTTTTCATCCATGGTTTCCCAGAGCTTTGGTATACATGGCGCCATCAGATTTTGGCCTTGAGCTCTCTTGGGTACCACGCTGTGGCGCCGGACCTTCGTGGGTATGGCGATTCTGATGCCCCTGATTCCATTTCTAGCTATTCTATTATGCATATTGTTGGGGATCTTGTTGCTCTTGTTGAGAGTTTTGGGGTTAAGGAAGTTTTCGTTGTGGCGCATGATTGGGGCGCTTTGATTGCATGGTCTTTGTGTTTATTCCGAGCTGAGATGGTTAAGGCGTTTGTGTGTCTAAGTGTGCCGTTTAGGCCTAGGCATCCGAACAGGAAGCCTGTTGAGACCATGAGGATGGTTTTTGGAGATGATTACTACATCTGCAGATTCCAGAATCCAGGAGAGATTGAAGAAGAGATGGCTCAAGTGGGTGCCAAAGACGTACTAAGAGGCATATTAACAACAAGAAGACAAGGGCCTCCCATTTATCCCAAAAAACAAGCCTTCCGAGCCAGGGCCGGACCCCCATCTCCCTTGCCCTCTTGGCTTTCAGAAGAAGATCTTTCTTACTTTGCCTCCAAATATGAGCAGAAGGGATTCACTGGACCATTGAACTATTACAGATCAATGGACTTAAACTGGGAGTTAACAGCACCATGGACAGGGGTTCAAGTGAAAGTGCCAGTGAAGTTCATAGTTGGAGATGTTGACATGGTCTATACAACACCAGGAGTTAAGGAGTATGTCAATGGTGGCGGTTTCAAGAAAGATGTGCCATTTCTGCAAGACGTTGTGGTAATGGATGGAGTTGGCCACTTCCTAAACCAAGAGAAGCCTGAAGAGATTAACACTCACATCTATGACTTCATCAAAAAATTCTAG

Protein sequence

MDTISHRTVSVNGINMHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLRGYGDSDAPDSISSYSIMHIVGDLVALVESFGVKEVFVVAHDWGALIAWSLCLFRAEMVKAFVCLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVLRGILTTRRQGPPIYPKKQAFRARAGPPSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWELTAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVGHFLNQEKPEEINTHIYDFIKKF*
Homology
BLAST of CsGy1G025560 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 6.0e-60
Identity = 125/326 (38.34%), Postives = 179/326 (54.91%), Query Frame = 0

Query: 1   MDTISHRTVSVNGINMHIAEKGE--GPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPD 60
           M   + R V  NG+ + + E GE   P+V+  HGFPEL Y+WRHQI AL+  GYH +APD
Sbjct: 1   MGAPTERLVDTNGVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPD 60

Query: 61  LRGYGDSDAPDSISSYSIMHIVGDLVALVESFGVKEVFVVAHDWGALIAWSLCLFRAEMV 120
            RGYG S  P++I +Y I  +  DLV L++  G +    V HDWGA++ W+  L  A+ V
Sbjct: 61  QRGYGGSSRPEAIEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRV 120

Query: 121 KAFVCLSVPFRPRHPNRKPVETMRMVFGDD-YYICRFQNPGEIEEEMAQVGAKDVLRGIL 180
            A   LSVP  PR     P +  R  FG++ +YI  FQ PG  + E+    A+ + R I 
Sbjct: 121 AAVAALSVPALPR-AQVPPTQAFRSRFGENFFYILYFQEPGIADAELNGDPARTMRRMIG 180

Query: 181 TTRRQGPP------IYPKKQAFRARAGPPSPLPSWLSEEDLSYFASKYEQKGFTGPLNYY 240
             R  G        + P    F  R   P+ LP+W+S+E+L ++  ++ + GFTG LN+Y
Sbjct: 181 GLRPPGDQSAAMRMLAPGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGLNWY 240

Query: 241 RSMDLNWELTAPWTGVQVKVPVKFIVGDVDMVYT---TPGVKEYVNGGGFKKDVPFLQDV 300
           R+ D NWE TA   G  + VP  FI G  D V T   T    E ++G       P+ +  
Sbjct: 241 RNFDRNWETTADLAGKTISVPSLFIAGTADPVLTFTRTDRAAEVISG-------PYRE-- 300

Query: 301 VVMDGVGHFLNQEKPEEINTHIYDFI 315
           V++DG GH+L QE+P E+   + +F+
Sbjct: 301 VLIDGAGHWLQQERPGEVTAALLEFL 316

BLAST of CsGy1G025560 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 3.4e-47
Identity = 103/320 (32.19%), Postives = 168/320 (52.50%), Query Frame = 0

Query: 4   ISHRTVSVN-GINMHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLRGY 63
           +SH  V +  G+ +H  E G GP V   HGFPE W++WR+QI AL+  G+  +A D++GY
Sbjct: 237 LSHGYVLIKPGVRLHFVEMGSGPAVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKGY 296

Query: 64  GDSDAPDSISSYSIMHIVGDLVALVESFGVKEVFVVAHDWGALIAWSLCLFRAEMVKAFV 123
           G+S AP  I  YS+  +  D+V  +   G+ +   + HDWG ++ W++ LF  E V+A  
Sbjct: 297 GESSAPPEIEEYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAVA 356

Query: 124 CLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQ---VGAKDVLRG---- 183
            L+ PF P +PN  P+E ++     DY +  FQ PG  E E+ Q      K+  R     
Sbjct: 357 SLNTPFMPSNPNVSPMEIIKANPVFDYQL-YFQEPGVAEAELEQNLDRTFKNFFRAHDET 416

Query: 184 ILTTRRQGPPIYPKKQAFRARAGPPSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMD 243
            LTT R    +      F      PS L   ++EED+ ++  ++++ GF GPLN+YR+M+
Sbjct: 417 FLTTNR----VRELGGLFVGTPEEPS-LSRLVTEEDIQFYVQQFKKSGFRGPLNWYRNME 476

Query: 244 LNWELTAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVG 303
            NW+     +G ++ +P   +  + D+V      K   N       +P L+   + D  G
Sbjct: 477 RNWQWGCKGSGRKILIPALMVTAENDLVLHPKMSKHMENW------IPHLKRGHIKD-CG 536

Query: 304 HFLNQEKPEEINTHIYDFIK 316
           H+   +KP E+N  + ++++
Sbjct: 537 HWTQIDKPAELNRILIEWLE 543

BLAST of CsGy1G025560 vs. ExPASy Swiss-Prot
Match: P80299 (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 4.2e-45
Identity = 97/316 (30.70%), Postives = 163/316 (51.58%), Query Frame = 0

Query: 4   ISHRTVSVN-GINMHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLRGY 63
           +SH  V+V  GI +H  E G GP +   HGFPE W++WR+QI AL+  G+  +A D++GY
Sbjct: 235 VSHGYVTVKPGIRLHFVEMGSGPAICLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGY 294

Query: 64  GDSDAPDSISSYSIMHIVGDLVALVESFGVKEVFVVAHDWGALIAWSLCLFRAEMVKAFV 123
           GDS +P  I  Y++  +  ++V  +   G+ +   + HDW  ++ W++ LF  E V+A  
Sbjct: 295 GDSSSPPEIEEYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVRAVA 354

Query: 124 CLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVLRGILTTRRQ 183
            L+ P  P +P   P+E +R +   +Y +  FQ PG  E E+ +  ++       T+   
Sbjct: 355 SLNTPLMPPNPEVSPMEVIRSIPVFNYQL-YFQEPGVAEAELEKNMSRTFKSFFRTSDDM 414

Query: 184 GPPIYPKKQAFRA--RAGPPSPLPSWL-SEEDLSYFASKYEQKGFTGPLNYYRSMDLNWE 243
           G     K           P  P  S + +EE++ Y+  ++++ GF GPLN+YR+ + NW+
Sbjct: 415 GLLTVNKATEMGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNWYRNTERNWK 474

Query: 244 LTAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVGHFLN 303
            +    G ++ VP   +  + D+V      K   N       +PFL+   + D  GH+  
Sbjct: 475 WSCKALGRKILVPALMVTAEKDIVLRPEMSKNMENW------IPFLKRGHIED-CGHWTQ 534

Query: 304 QEKPEEINTHIYDFIK 316
            EKP E+N  +  ++K
Sbjct: 535 IEKPAEVNQILIKWLK 542

BLAST of CsGy1G025560 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 182.2 bits (461), Expect = 9.3e-45
Identity = 94/307 (30.62%), Postives = 161/307 (52.44%), Query Frame = 0

Query: 4   ISHRTVSVN-GINMHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLRGY 63
           +SH  V+V   + +H  E G GP V   HGFPE WY+WR+QI AL+  GY  +A D++GY
Sbjct: 237 MSHGYVTVKPRVRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGY 296

Query: 64  GDSDAPDSISSYSIMHIVGDLVALVESFGVKEVFVVAHDWGALIAWSLCLFRAEMVKAFV 123
           G+S AP  I  Y +  +  ++V  ++  G+ +   + HDWG ++ W + LF  E V+A  
Sbjct: 297 GESSAPPEIEEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVA 356

Query: 124 CLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVLRGILTTRRQ 183
            L+ PF P +PN  P+E+++     DY +  FQ PG  E E+ Q  ++        +   
Sbjct: 357 SLNTPFIPANPNMSPLESIKANPVFDYQL-YFQEPGVAEAELEQNLSRTFKSLFRASDES 416

Query: 184 GPPIYPKKQAFRARAGPPS--PLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 243
              ++   +A       P    L   ++EE++ ++  ++++ GF GPLN+YR+M+ NW+ 
Sbjct: 417 VLSMHKVCEAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKW 476

Query: 244 TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVGHFLNQ 303
                G ++ +P   +  + D V   P + +++        +P L+   + D  GH+   
Sbjct: 477 ACKSLGRKILIPALMVTAEKDFV-LVPQMSQHMEDW-----IPHLKRGHIED-CGHWTQM 535

Query: 304 EKPEEIN 308
           +KP E+N
Sbjct: 537 DKPTEVN 535

BLAST of CsGy1G025560 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 177.6 bits (449), Expect = 2.3e-43
Identity = 94/308 (30.52%), Postives = 160/308 (51.95%), Query Frame = 0

Query: 4   ISHRTVSVN-GINMHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLRGY 63
           +SH  V+V  GI +H  E G GP +   HGFPE W++WR+QI AL+  G+  +A D++GY
Sbjct: 235 VSHGYVTVKPGIRLHFVEMGSGPALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGY 294

Query: 64  GDSDAPDSISSYSIMHIVGDLVALVESFGVKEVFVVAHDWGALIAWSLCLFRAEMVKAFV 123
           GDS +P  I  Y++  +  ++V  ++  G+ +   + HDW  ++ W++ LF  E V+A  
Sbjct: 295 GDSSSPPEIEEYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVRAVA 354

Query: 124 CLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVLRGILTTRRQ 183
            L+ PF P  P+  P++ +R +   +Y +  FQ PG  E E+ +  ++        +   
Sbjct: 355 SLNTPFMPPDPDVSPMKVIRSIPVFNYQL-YFQEPGVAEAELEKNMSRTFKSFFRASDET 414

Query: 184 GPPIYPKKQAFRARA--GPPSP-LPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWE 243
           G     K           P  P L    +EE++ ++  ++++ GF GPLN+YR+ + NW+
Sbjct: 415 GFIAVHKATEIGGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYRNTERNWK 474

Query: 244 LTAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVGHFLN 303
            +    G ++ VP   +  + D+V      K        +K +PFL+   + D  GH+  
Sbjct: 475 WSCKGLGRKILVPALMVTAEKDIVLRPEMSK------NMEKWIPFLKRGHIED-CGHWTQ 534

Query: 304 QEKPEEIN 308
            EKP E+N
Sbjct: 535 IEKPTEVN 534

BLAST of CsGy1G025560 vs. NCBI nr
Match: XP_011659835.1 (uncharacterized protein LOC101203211 [Cucumis sativus] >KGN66047.1 hypothetical protein Csa_006919 [Cucumis sativus])

HSP 1 Score: 667 bits (1721), Expect = 2.51e-242
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0

Query: 1   MDTISHRTVSVNGINMHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR 60
           MDTISHRTVSVNGINMHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR
Sbjct: 1   MDTISHRTVSVNGINMHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR 60

Query: 61  GYGDSDAPDSISSYSIMHIVGDLVALVESFGVKEVFVVAHDWGALIAWSLCLFRAEMVKA 120
           GYGDSDAPDSISSYSIMHIVGDLVALVESFGVKEVFVVAHDWGALIAWSLCLFRAEMVKA
Sbjct: 61  GYGDSDAPDSISSYSIMHIVGDLVALVESFGVKEVFVVAHDWGALIAWSLCLFRAEMVKA 120

Query: 121 FVCLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVLRGILTTR 180
           FVCLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVLRGILTTR
Sbjct: 121 FVCLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVLRGILTTR 180

Query: 181 RQGPPIYPKKQAFRARAGPPSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240
           RQGPPIYPKKQAFRARAGPPSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL
Sbjct: 181 RQGPPIYPKKQAFRARAGPPSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240

Query: 241 TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVGHFLNQ 300
           TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVGHFLNQ
Sbjct: 241 TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVGHFLNQ 300

Query: 301 EKPEEINTHIYDFIKKF 317
           EKPEEINTHIYDFIKKF
Sbjct: 301 EKPEEINTHIYDFIKKF 317

BLAST of CsGy1G025560 vs. NCBI nr
Match: XP_008450607.1 (PREDICTED: bifunctional epoxide hydrolase 2-like isoform X1 [Cucumis melo] >KAA0050918.1 bifunctional epoxide hydrolase 2-like isoform X1 [Cucumis melo var. makuwa] >TYK10266.1 bifunctional epoxide hydrolase 2-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 641 bits (1653), Expect = 5.86e-232
Identity = 303/317 (95.58%), Postives = 310/317 (97.79%), Query Frame = 0

Query: 1   MDTISHRTVSVNGINMHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR 60
           MDTI+HRTVSVNGIN+HIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR
Sbjct: 1   MDTINHRTVSVNGINVHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR 60

Query: 61  GYGDSDAPDSISSYSIMHIVGDLVALVESFGVKEVFVVAHDWGALIAWSLCLFRAEMVKA 120
           GYGDSDAPDSISSYSIMHIVGDLVALVES GVKEVFVVAHDWGALIAW LCLFRAE+VKA
Sbjct: 61  GYGDSDAPDSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWGLCLFRAEIVKA 120

Query: 121 FVCLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVLRGILTTR 180
           FVCLSVPFRPRHPNRKPVETMR VFGDDYYICRFQNPGEIEEEMAQVGAK+VLRGILTTR
Sbjct: 121 FVCLSVPFRPRHPNRKPVETMRKVFGDDYYICRFQNPGEIEEEMAQVGAKEVLRGILTTR 180

Query: 181 RQGPPIYPKKQAFRARAGPPSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240
           RQGPPIYPKKQAFRAR    SPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL
Sbjct: 181 RQGPPIYPKKQAFRARPESSSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240

Query: 241 TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVGHFLNQ 300
           TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVV+M+GVGHFLNQ
Sbjct: 241 TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVIMEGVGHFLNQ 300

Query: 301 EKPEEINTHIYDFIKKF 317
           EKPEEINTHIYDFI+KF
Sbjct: 301 EKPEEINTHIYDFIRKF 317

BLAST of CsGy1G025560 vs. NCBI nr
Match: XP_038879653.1 (epoxide hydrolase A-like [Benincasa hispida])

HSP 1 Score: 613 bits (1582), Expect = 3.90e-221
Identity = 290/317 (91.48%), Postives = 300/317 (94.64%), Query Frame = 0

Query: 1   MDTISHRTVSVNGINMHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR 60
           MDTI HRTVSVNGINMHIAEKGEGPIVL IHGFPELWY WRHQILALSSLGY AVAPDLR
Sbjct: 1   MDTIEHRTVSVNGINMHIAEKGEGPIVLLIHGFPELWYAWRHQILALSSLGYRAVAPDLR 60

Query: 61  GYGDSDAPDSISSYSIMHIVGDLVALVESFGVKEVFVVAHDWGALIAWSLCLFRAEMVKA 120
           GYGDSDAPDSISSYSIMHIVGDLVALVES GVKEVFVVAHDWGALIAW LCLFRAE+VKA
Sbjct: 61  GYGDSDAPDSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWCLCLFRAEIVKA 120

Query: 121 FVCLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVLRGILTTR 180
           FVCLSVPFRPRHP  KPVETMR  FGDDYYICRFQNPGEIEEEMAQVGAK+VL+GILTTR
Sbjct: 121 FVCLSVPFRPRHPYMKPVETMRKFFGDDYYICRFQNPGEIEEEMAQVGAKEVLKGILTTR 180

Query: 181 RQGPPIYPKKQAFRARAGPPSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240
           R GPPI PK QAFRAR G  SPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL
Sbjct: 181 RPGPPILPKGQAFRARPGSSSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240

Query: 241 TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVGHFLNQ 300
           TAPWTGVQVKVPVKFIVGD DMVYTTPG+KEYVNGGGFKKDVPFLQ+VV+M+GVGHFLNQ
Sbjct: 241 TAPWTGVQVKVPVKFIVGDADMVYTTPGIKEYVNGGGFKKDVPFLQEVVIMEGVGHFLNQ 300

Query: 301 EKPEEINTHIYDFIKKF 317
           EKPEEI+THI+DFI+KF
Sbjct: 301 EKPEEISTHIHDFIRKF 317

BLAST of CsGy1G025560 vs. NCBI nr
Match: XP_022969836.1 (uncharacterized protein LOC111468917 [Cucurbita maxima])

HSP 1 Score: 589 bits (1519), Expect = 1.56e-211
Identity = 275/317 (86.75%), Postives = 296/317 (93.38%), Query Frame = 0

Query: 1   MDTISHRTVSVNGINMHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR 60
           MD+I HRTVSVNGINMHIAEKGEGPIVL IHGFPELWY+WRHQILALSS GY AVAPDLR
Sbjct: 1   MDSIQHRTVSVNGINMHIAEKGEGPIVLLIHGFPELWYSWRHQILALSSHGYRAVAPDLR 60

Query: 61  GYGDSDAPDSISSYSIMHIVGDLVALVESFGVKEVFVVAHDWGALIAWSLCLFRAEMVKA 120
           GYGDSDAPDS+SSYSIMHIVGDLVALVE  GVKEVFVVAHDWGA+IAW LCLFRA++VKA
Sbjct: 61  GYGDSDAPDSLSSYSIMHIVGDLVALVEILGVKEVFVVAHDWGAMIAWCLCLFRADIVKA 120

Query: 121 FVCLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVLRGILTTR 180
           FVCLSVPFRPRHP  KPVE+MR  FGDDYYICRFQNPGEIEEEMA VGAK+V+RGILTTR
Sbjct: 121 FVCLSVPFRPRHPKVKPVESMRKFFGDDYYICRFQNPGEIEEEMAMVGAKEVVRGILTTR 180

Query: 181 RQGPPIYPKKQAFRARAGPPSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240
           R GPPI+PK QAF++R    +PLPSWLSEEDLSYFASK+E KGFTGPLNYYRSMDLNWEL
Sbjct: 181 RTGPPIFPKGQAFKSRPESSTPLPSWLSEEDLSYFASKFESKGFTGPLNYYRSMDLNWEL 240

Query: 241 TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVGHFLNQ 300
           TAPWTGVQVKVPVKF+VGDVDMVYTTPGVKEYVNGGGFKKDVP+LQ+VVVM+GVGHFL Q
Sbjct: 241 TAPWTGVQVKVPVKFVVGDVDMVYTTPGVKEYVNGGGFKKDVPYLQEVVVMEGVGHFLTQ 300

Query: 301 EKPEEINTHIYDFIKKF 317
           EKPEE+++HI DFIKKF
Sbjct: 301 EKPEEVSSHILDFIKKF 317

BLAST of CsGy1G025560 vs. NCBI nr
Match: XP_023531428.1 (uncharacterized protein LOC111793669 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 588 bits (1516), Expect = 4.48e-211
Identity = 274/317 (86.44%), Postives = 295/317 (93.06%), Query Frame = 0

Query: 1   MDTISHRTVSVNGINMHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR 60
           MD+I HRTVSVNGINMHIAEKGEGPIVL IHGFPELWY+WRHQILALSS GY AVAPDLR
Sbjct: 1   MDSIQHRTVSVNGINMHIAEKGEGPIVLLIHGFPELWYSWRHQILALSSQGYRAVAPDLR 60

Query: 61  GYGDSDAPDSISSYSIMHIVGDLVALVESFGVKEVFVVAHDWGALIAWSLCLFRAEMVKA 120
           GYGDSDAPDS+SSYSIMHIVGDLVALVE  GVKEVFVVAHDWGA+IAW LCLFR ++VKA
Sbjct: 61  GYGDSDAPDSLSSYSIMHIVGDLVALVEILGVKEVFVVAHDWGAMIAWCLCLFRPDIVKA 120

Query: 121 FVCLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVLRGILTTR 180
           FVCLSVPFRPRHP  KPVE+MR  FGDDYYICRFQNPGEIEEEMA VGAK+V+RGILTTR
Sbjct: 121 FVCLSVPFRPRHPKIKPVESMRKFFGDDYYICRFQNPGEIEEEMAVVGAKEVVRGILTTR 180

Query: 181 RQGPPIYPKKQAFRARAGPPSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240
           + GPPI+PK QAF+ R G  +PLPSWLSEEDLSYFASK+E KGFTGPLNYYRSMDLNWEL
Sbjct: 181 KTGPPIFPKGQAFQVRPGSSTPLPSWLSEEDLSYFASKFESKGFTGPLNYYRSMDLNWEL 240

Query: 241 TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVGHFLNQ 300
           TAPWTGVQVKVPVKF+VGDVDMVYTTPGVKEYVNGGGFKKDVP+LQ+VVVM+GVGHFL Q
Sbjct: 241 TAPWTGVQVKVPVKFVVGDVDMVYTTPGVKEYVNGGGFKKDVPYLQEVVVMEGVGHFLTQ 300

Query: 301 EKPEEINTHIYDFIKKF 317
           EKPEE+++HI DFIKKF
Sbjct: 301 EKPEEVSSHILDFIKKF 317

BLAST of CsGy1G025560 vs. ExPASy TrEMBL
Match: A0A0A0LYZ6 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G569240 PE=4 SV=1)

HSP 1 Score: 667 bits (1721), Expect = 1.22e-242
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0

Query: 1   MDTISHRTVSVNGINMHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR 60
           MDTISHRTVSVNGINMHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR
Sbjct: 1   MDTISHRTVSVNGINMHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR 60

Query: 61  GYGDSDAPDSISSYSIMHIVGDLVALVESFGVKEVFVVAHDWGALIAWSLCLFRAEMVKA 120
           GYGDSDAPDSISSYSIMHIVGDLVALVESFGVKEVFVVAHDWGALIAWSLCLFRAEMVKA
Sbjct: 61  GYGDSDAPDSISSYSIMHIVGDLVALVESFGVKEVFVVAHDWGALIAWSLCLFRAEMVKA 120

Query: 121 FVCLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVLRGILTTR 180
           FVCLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVLRGILTTR
Sbjct: 121 FVCLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVLRGILTTR 180

Query: 181 RQGPPIYPKKQAFRARAGPPSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240
           RQGPPIYPKKQAFRARAGPPSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL
Sbjct: 181 RQGPPIYPKKQAFRARAGPPSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240

Query: 241 TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVGHFLNQ 300
           TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVGHFLNQ
Sbjct: 241 TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVGHFLNQ 300

Query: 301 EKPEEINTHIYDFIKKF 317
           EKPEEINTHIYDFIKKF
Sbjct: 301 EKPEEINTHIYDFIKKF 317

BLAST of CsGy1G025560 vs. ExPASy TrEMBL
Match: A0A5D3CIS4 (Bifunctional epoxide hydrolase 2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004160 PE=4 SV=1)

HSP 1 Score: 641 bits (1653), Expect = 2.84e-232
Identity = 303/317 (95.58%), Postives = 310/317 (97.79%), Query Frame = 0

Query: 1   MDTISHRTVSVNGINMHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR 60
           MDTI+HRTVSVNGIN+HIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR
Sbjct: 1   MDTINHRTVSVNGINVHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR 60

Query: 61  GYGDSDAPDSISSYSIMHIVGDLVALVESFGVKEVFVVAHDWGALIAWSLCLFRAEMVKA 120
           GYGDSDAPDSISSYSIMHIVGDLVALVES GVKEVFVVAHDWGALIAW LCLFRAE+VKA
Sbjct: 61  GYGDSDAPDSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWGLCLFRAEIVKA 120

Query: 121 FVCLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVLRGILTTR 180
           FVCLSVPFRPRHPNRKPVETMR VFGDDYYICRFQNPGEIEEEMAQVGAK+VLRGILTTR
Sbjct: 121 FVCLSVPFRPRHPNRKPVETMRKVFGDDYYICRFQNPGEIEEEMAQVGAKEVLRGILTTR 180

Query: 181 RQGPPIYPKKQAFRARAGPPSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240
           RQGPPIYPKKQAFRAR    SPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL
Sbjct: 181 RQGPPIYPKKQAFRARPESSSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240

Query: 241 TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVGHFLNQ 300
           TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVV+M+GVGHFLNQ
Sbjct: 241 TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVIMEGVGHFLNQ 300

Query: 301 EKPEEINTHIYDFIKKF 317
           EKPEEINTHIYDFI+KF
Sbjct: 301 EKPEEINTHIYDFIRKF 317

BLAST of CsGy1G025560 vs. ExPASy TrEMBL
Match: A0A1S3BNY7 (bifunctional epoxide hydrolase 2-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492144 PE=4 SV=1)

HSP 1 Score: 641 bits (1653), Expect = 2.84e-232
Identity = 303/317 (95.58%), Postives = 310/317 (97.79%), Query Frame = 0

Query: 1   MDTISHRTVSVNGINMHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR 60
           MDTI+HRTVSVNGIN+HIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR
Sbjct: 1   MDTINHRTVSVNGINVHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR 60

Query: 61  GYGDSDAPDSISSYSIMHIVGDLVALVESFGVKEVFVVAHDWGALIAWSLCLFRAEMVKA 120
           GYGDSDAPDSISSYSIMHIVGDLVALVES GVKEVFVVAHDWGALIAW LCLFRAE+VKA
Sbjct: 61  GYGDSDAPDSISSYSIMHIVGDLVALVESLGVKEVFVVAHDWGALIAWGLCLFRAEIVKA 120

Query: 121 FVCLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVLRGILTTR 180
           FVCLSVPFRPRHPNRKPVETMR VFGDDYYICRFQNPGEIEEEMAQVGAK+VLRGILTTR
Sbjct: 121 FVCLSVPFRPRHPNRKPVETMRKVFGDDYYICRFQNPGEIEEEMAQVGAKEVLRGILTTR 180

Query: 181 RQGPPIYPKKQAFRARAGPPSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240
           RQGPPIYPKKQAFRAR    SPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL
Sbjct: 181 RQGPPIYPKKQAFRARPESSSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240

Query: 241 TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVGHFLNQ 300
           TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVV+M+GVGHFLNQ
Sbjct: 241 TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVIMEGVGHFLNQ 300

Query: 301 EKPEEINTHIYDFIKKF 317
           EKPEEINTHIYDFI+KF
Sbjct: 301 EKPEEINTHIYDFIRKF 317

BLAST of CsGy1G025560 vs. ExPASy TrEMBL
Match: A0A6J1HYW5 (uncharacterized protein LOC111468917 OS=Cucurbita maxima OX=3661 GN=LOC111468917 PE=4 SV=1)

HSP 1 Score: 589 bits (1519), Expect = 7.56e-212
Identity = 275/317 (86.75%), Postives = 296/317 (93.38%), Query Frame = 0

Query: 1   MDTISHRTVSVNGINMHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR 60
           MD+I HRTVSVNGINMHIAEKGEGPIVL IHGFPELWY+WRHQILALSS GY AVAPDLR
Sbjct: 1   MDSIQHRTVSVNGINMHIAEKGEGPIVLLIHGFPELWYSWRHQILALSSHGYRAVAPDLR 60

Query: 61  GYGDSDAPDSISSYSIMHIVGDLVALVESFGVKEVFVVAHDWGALIAWSLCLFRAEMVKA 120
           GYGDSDAPDS+SSYSIMHIVGDLVALVE  GVKEVFVVAHDWGA+IAW LCLFRA++VKA
Sbjct: 61  GYGDSDAPDSLSSYSIMHIVGDLVALVEILGVKEVFVVAHDWGAMIAWCLCLFRADIVKA 120

Query: 121 FVCLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVLRGILTTR 180
           FVCLSVPFRPRHP  KPVE+MR  FGDDYYICRFQNPGEIEEEMA VGAK+V+RGILTTR
Sbjct: 121 FVCLSVPFRPRHPKVKPVESMRKFFGDDYYICRFQNPGEIEEEMAMVGAKEVVRGILTTR 180

Query: 181 RQGPPIYPKKQAFRARAGPPSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240
           R GPPI+PK QAF++R    +PLPSWLSEEDLSYFASK+E KGFTGPLNYYRSMDLNWEL
Sbjct: 181 RTGPPIFPKGQAFKSRPESSTPLPSWLSEEDLSYFASKFESKGFTGPLNYYRSMDLNWEL 240

Query: 241 TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVGHFLNQ 300
           TAPWTGVQVKVPVKF+VGDVDMVYTTPGVKEYVNGGGFKKDVP+LQ+VVVM+GVGHFL Q
Sbjct: 241 TAPWTGVQVKVPVKFVVGDVDMVYTTPGVKEYVNGGGFKKDVPYLQEVVVMEGVGHFLTQ 300

Query: 301 EKPEEINTHIYDFIKKF 317
           EKPEE+++HI DFIKKF
Sbjct: 301 EKPEEVSSHILDFIKKF 317

BLAST of CsGy1G025560 vs. ExPASy TrEMBL
Match: A0A6J1HVS6 (uncharacterized protein LOC111467903 OS=Cucurbita maxima OX=3661 GN=LOC111467903 PE=4 SV=1)

HSP 1 Score: 588 bits (1516), Expect = 2.33e-211
Identity = 278/319 (87.15%), Postives = 297/319 (93.10%), Query Frame = 0

Query: 1   MDTISHRTVSVNGINMHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR 60
           MDTI HRTVSVNGINMH+AEKGEGP+VL IHGFPELWY+WRHQILALSSLGY AVAPDLR
Sbjct: 1   MDTIQHRTVSVNGINMHVAEKGEGPVVLLIHGFPELWYSWRHQILALSSLGYRAVAPDLR 60

Query: 61  GYGDSDAPDSISSYSIMHIVGDLVALVESFGVKEVFVVAHDWGALIAWSLCLFRAEMVKA 120
           GYGDSD+PDSISSYSIMHIVGDLVALVES GVK+VFVVAHDWGA+IAWSLC FRAE+VKA
Sbjct: 61  GYGDSDSPDSISSYSIMHIVGDLVALVESLGVKQVFVVAHDWGAIIAWSLCSFRAEIVKA 120

Query: 121 FVCLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKD--VLRGILT 180
           FVCLSVPFRPR+P  KPVET R VFGDDYYICRFQNPGEIEEEMA+VGAK   VL+GILT
Sbjct: 121 FVCLSVPFRPRNPMMKPVETFRKVFGDDYYICRFQNPGEIEEEMAEVGAKAKAVLQGILT 180

Query: 181 TRRQGPPIYPKKQAFRARAGPPSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNW 240
           TR  GP I PK QAF+AR G P PLPSWLSE+DLSYFASKYEQKGFTGPLNYYRSMDLNW
Sbjct: 181 TRSPGPLILPKGQAFKARPGSPPPLPSWLSEQDLSYFASKYEQKGFTGPLNYYRSMDLNW 240

Query: 241 ELTAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVGHFL 300
           ELTAPWTGV+VKVPVK+IVGD D+VYTTPGVKEYVNGGGFKK+VPFLQ+VV+M+GVGHFL
Sbjct: 241 ELTAPWTGVEVKVPVKYIVGDADIVYTTPGVKEYVNGGGFKKNVPFLQEVVIMEGVGHFL 300

Query: 301 NQEKPEEINTHIYDFIKKF 317
           NQEKPEEINTHI DFIKKF
Sbjct: 301 NQEKPEEINTHILDFIKKF 319

BLAST of CsGy1G025560 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 446.8 bits (1148), Expect = 1.4e-125
Identity = 195/317 (61.51%), Postives = 248/317 (78.23%), Query Frame = 0

Query: 1   MDTISHRTVSVNGINMHIAEKGEGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLR 60
           M+ I H T+S NGINMH+A  G GP++LF+HGFP+LWY+WRHQ+++ ++LGY A+APDLR
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSGPVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDLR 60

Query: 61  GYGDSDAPDSISSYSIMHIVGDLVALVESFGVKEVFVVAHDWGALIAWSLCLFRAEMVKA 120
           GYGDSDAP S  SY+I+HIVGDLV L++S GV  VF+V HDWGA++AW LC+ R + V A
Sbjct: 61  GYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRVNA 120

Query: 121 FVCLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVLRGILTTR 180
            V  SV F PR+P+ KPV+  R +FGDDYYICRFQ PGEIEE+ AQV  K ++    T+R
Sbjct: 121 LVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRFFTSR 180

Query: 181 RQGPPIYPKKQAFRARAGPPSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLNWEL 240
              PP  PK   FR    PPS LP+WL+E+D+ ++  K+ QKGFTG LNYYR+++L+WEL
Sbjct: 181 NPRPPCIPKSVGFRGLPDPPS-LPAWLTEQDVRFYGDKFSQKGFTGGLNYYRALNLSWEL 240

Query: 241 TAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVGHFLNQ 300
           TAPWTG+Q+KVPVKFIVGD+D+ Y  PG KEY++ GG KK VPFLQ+VVVM+GVGHFL+Q
Sbjct: 241 TAPWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHFLHQ 300

Query: 301 EKPEEINTHIYDFIKKF 318
           EKP+E+  HIY F KKF
Sbjct: 301 EKPDEVTDHIYGFFKKF 316

BLAST of CsGy1G025560 vs. TAIR 10
Match: AT3G05600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 436.8 bits (1122), Expect = 1.5e-122
Identity = 200/323 (61.92%), Postives = 247/323 (76.47%), Query Frame = 0

Query: 1   MDTISHRTVSVNGINMHIAEKG--EGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPD 60
           M+ I HR VSVNGI MHIAEKG  EGP+VL +HGFP+LWYTWRHQI  LSSLGY AVAPD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 61  LRGYGDSDAPDSISSYSIMHIVGDLVALVESF--GVKEVFVVAHDWGALIAWSLCLFRAE 120
           LRGYGDSD+P+S S Y+ +++VGDLVAL++S     ++VF+V HDWGA+I W LCLFR E
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 121 MVKAFVCLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVLRGI 180
            +  FVCLSVP+R R+P  KPV+  + VFGDDYYICRFQ PG+IE E+A    +  LR +
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRNL 180

Query: 181 LTTRRQGPPIYPKKQAFRARAGPPS---PLPSWLSEEDLSYFASKYEQKGFTGPLNYYRS 240
            T R  GPPI PK   F  +  P S    LP W S++DL ++ SK+E+ GFTG LNYYR+
Sbjct: 181 FTGRTLGPPILPKDNPFGEKPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNYYRA 240

Query: 241 MDLNWELTAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDG 300
           MDLNWELTAPWTG +++VPVKF+ GD DMVYTTPG+KEY++GGGF  DVP LQ++VV++ 
Sbjct: 241 MDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVIED 300

Query: 301 VGHFLNQEKPEEINTHIYDFIKK 317
            GHF+NQEKP+E+  HI DF  K
Sbjct: 301 AGHFVNQEKPQEVTAHINDFFTK 323

BLAST of CsGy1G025560 vs. TAIR 10
Match: AT4G15960.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 424.5 bits (1090), Expect = 7.6e-119
Identity = 188/324 (58.02%), Postives = 246/324 (75.93%), Query Frame = 0

Query: 1   MDTISHRTVSVNGINMHIAEK-----GEGPIVLFIHGFPELWYTWRHQILALSSLGYHAV 60
           +D + H+T+ VNGINMH+AEK     GE PI+LF+HGFPELWYTWRHQ++ALSSLGY  +
Sbjct: 51  LDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGYRTI 110

Query: 61  APDLRGYGDSDAPDSISSYSIMHIVGDLVALVESF--GVKEVFVVAHDWGALIAWSLCLF 120
           APDLRGYGD++AP+ +  Y+ +++ GD+VAL+++   G K V VV HDWGA+IAW LC +
Sbjct: 111 APDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMIAWQLCQY 170

Query: 121 RAEMVKAFVCLSVPFRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVL 180
           R E VKA V +SV F PR+P R PV T+R VFGDDYY+CRFQ  GEIE E  ++G ++VL
Sbjct: 171 RPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEFKKLGTENVL 230

Query: 181 RGILTTRRQGPPIYPKKQAFRARAGPPSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRS 240
           +  LT +  GP   PK + F+      S LP WL++EDL Y+ +KYE KGFTGP+NYYR+
Sbjct: 231 KEFLTYKTPGPLNLPKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGFTGPINYYRN 290

Query: 241 MDLNWELTAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDG 300
           +D NWELTAPWTG +++VPVKFI+GD D+ Y  PG KEY+NGGGFK+DVP L + VV+ G
Sbjct: 291 IDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLDETVVLKG 350

Query: 301 VGHFLNQEKPEEINTHIYDFIKKF 318
           +GHFL++E P+ IN HI++F  KF
Sbjct: 351 LGHFLHEENPDVINQHIHNFFHKF 374

BLAST of CsGy1G025560 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 401.0 bits (1029), Expect = 9.1e-112
Identity = 188/321 (58.57%), Postives = 243/321 (75.70%), Query Frame = 0

Query: 4   ISHRTVSVNGINMHIAEKG--EGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLRG 63
           + HR V  NGI++H+A +G  +GPIVL +HGFPELWY+WRHQI  L++ GY AVAPDLRG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 64  YGDSDAPDSISSYSIMHIVGDLVALVESFGVKE---VFVVAHDWGALIAWSLCLFRAEMV 123
           YGDSDAP  ISSY+  +IVGDL+A++ +    E   VFVV HDWGALIAW LCLFR + V
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 124 KAFVCLSVP--FRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVLRGI 183
           KA V LSVP  FRP  P+ KPV+ MR  +GDDYYICRFQ  G++E E+A+VG + V++ +
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 184 LTTRRQGPPIYPKKQAFRARAGPPSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDL 243
           LT R  GP I PK ++F    G   PLPSWL+EED++YF SK+E+KGF+GP+NYYR+ + 
Sbjct: 181 LTYRTPGPVIIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFEEKGFSGPVNYYRNFNR 240

Query: 244 NWELTAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVGH 303
           N EL  PW G +++VP KF++G++D+VY  PGVKEY++G  FK+DVP L++ VVM+GV H
Sbjct: 241 NNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVMEGVAH 300

Query: 304 FLNQEKPEEINTHIYDFIKKF 318
           F+NQEKP+EI   I DFI KF
Sbjct: 301 FINQEKPQEILQIILDFISKF 321

BLAST of CsGy1G025560 vs. TAIR 10
Match: AT2G26750.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 389.8 bits (1000), Expect = 2.1e-108
Identity = 184/320 (57.50%), Postives = 241/320 (75.31%), Query Frame = 0

Query: 4   ISHRTVSVNGINMHIAEKG--EGPIVLFIHGFPELWYTWRHQILALSSLGYHAVAPDLRG 63
           + HR V  NGI++H+A +G  +G IVL +HGFPELWY+WRHQI  L++ GY AVAPDLRG
Sbjct: 1   MEHRNVRGNGIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAVAPDLRG 60

Query: 64  YGDSDAPDSISSYSIMHIVGDLVALVESF--GVKEVFVVAHDWGALIAWSLCLFRAEMVK 123
           YGDSDAP  ISS++  +IVGDLVA++ +     K+VFVV HDWGALIAW LCLFR + VK
Sbjct: 61  YGDSDAPAEISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWYLCLFRPDKVK 120

Query: 124 AFVCLSVP--FRPRHPNRKPVETMRMVFGDDYYICRFQNPGEIEEEMAQVGAKDVLRGIL 183
           A V LSVP  F P  P+ KPV+ MR V+G+DYY+CRFQ  G+IE E+A+VG + V++ +L
Sbjct: 121 ALVNLSVPLSFWPTDPSVKPVDRMRAVYGNDYYVCRFQEVGDIEAEIAEVGTERVMKRLL 180

Query: 184 TTRRQGPPIYPKKQAFRARAGPPSPLPSWLSEEDLSYFASKYEQKGFTGPLNYYRSMDLN 243
           T R  GP I PK ++F    G   PLPSWL+EED++YF SK+++KGF GP+NYYR+ + N
Sbjct: 181 TYRTPGPLIIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFKEKGFCGPVNYYRNFNRN 240

Query: 244 WELTAPWTGVQVKVPVKFIVGDVDMVYTTPGVKEYVNGGGFKKDVPFLQDVVVMDGVGHF 303
            EL  PW G +++VP KF++G++D+VY  PGVKEY++G  FK+DVP +++ VVM+GV HF
Sbjct: 241 NELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEGVAHF 300

Query: 304 LNQEKPEEINTHIYDFIKKF 318
           LNQEKP+EI   I DFI  F
Sbjct: 301 LNQEKPQEILQIILDFISTF 320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
I6YGS06.0e-6038.34Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX... [more]
Q6Q2C23.4e-4732.19Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1[more]
P802994.2e-4530.70Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=... [more]
P349139.3e-4530.62Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2[more]
P349142.3e-4330.52Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_011659835.12.51e-242100.00uncharacterized protein LOC101203211 [Cucumis sativus] >KGN66047.1 hypothetical ... [more]
XP_008450607.15.86e-23295.58PREDICTED: bifunctional epoxide hydrolase 2-like isoform X1 [Cucumis melo] >KAA0... [more]
XP_038879653.13.90e-22191.48epoxide hydrolase A-like [Benincasa hispida][more]
XP_022969836.11.56e-21186.75uncharacterized protein LOC111468917 [Cucurbita maxima][more]
XP_023531428.14.48e-21186.44uncharacterized protein LOC111793669 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0LYZ61.22e-242100.00AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G569... [more]
A0A5D3CIS42.84e-23295.58Bifunctional epoxide hydrolase 2-like isoform X1 OS=Cucumis melo var. makuwa OX=... [more]
A0A1S3BNY72.84e-23295.58bifunctional epoxide hydrolase 2-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1HYW57.56e-21286.75uncharacterized protein LOC111468917 OS=Cucurbita maxima OX=3661 GN=LOC111468917... [more]
A0A6J1HVS62.33e-21187.15uncharacterized protein LOC111467903 OS=Cucurbita maxima OX=3661 GN=LOC111467903... [more]
Match NameE-valueIdentityDescription
AT4G02340.11.4e-12561.51alpha/beta-Hydrolases superfamily protein [more]
AT3G05600.11.5e-12261.92alpha/beta-Hydrolases superfamily protein [more]
AT4G15960.17.6e-11958.02alpha/beta-Hydrolases superfamily protein [more]
AT2G26740.19.1e-11258.57soluble epoxide hydrolase [more]
AT2G26750.12.1e-10857.50alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 291..313
score: 44.66
coord: 31..49
score: 55.98
coord: 97..110
score: 55.84
coord: 51..66
score: 60.23
coord: 111..124
score: 38.96
coord: 251..267
score: 37.97
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..317
e-value: 3.9E-107
score: 360.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 3..316
IPR000073Alpha/beta hydrolase fold-1PFAMPF12697Abhydrolase_6coord: 27..307
e-value: 2.7E-21
score: 77.2
NoneNo IPR availablePANTHERPTHR43329:SF58OS05G0273800 PROTEINcoord: 1..317
NoneNo IPR availablePANTHERPTHR43329EPOXIDE HYDROLASEcoord: 1..317

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy1G025560.1CsGy1G025560.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004301 epoxide hydrolase activity
molecular_function GO:0003824 catalytic activity