CsGy1G025230 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy1G025230
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description3'-5' exonuclease domain-containing protein
LocationGy14Chr1: 24178581 .. 24186204 (-)
RNA-Seq ExpressionCsGy1G025230
SyntenyCsGy1G025230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAAGAAAAAAAAAAAAAGAAGAAAAAATTGATTTTGCCTAAATATTCAACCAATGTTTATTTTTTGTCCAACCTTCCTAGTTCCTCATAAGAAAGTTATCCAATGCAGAGTTTATCATGAAGACATTTTTTTGTCATACCATATTGGAGTTATTTCGGTTCATCTTCATTCTATTTATTTATATTCTACATTTTTAATGTCACCTAGTACAAATGCAGGCACATTGAAAGCAACTAAGAAGTTTCGATTCCTTCAGCAACAAGTTCATTTAGTACTTCATAATGGTCCCCAACCTGGACCAGCTACCTTTGTTATTCGTTGCCTATACGTCTTGCCTATATTTGGACTATATAGTGAAGGATTCAGCCACTTGATTACCTCAGCCCTCCAACGTTTTCTGAAAGTTGTGATAACTCCAGCAGACCTTGACGAAGCAAAGGACCTAGCTGCTCAGTTATTTATTGATATTGTTGGAGGATTCATTGCGCATGATGACAGGATAGTCGTGAAGATTATACAGATATTTGATGTTCAATTGTCCGATGTTGAGAAAGTTATGTTTGAATCTAAGGCAAGAAATAGGTGTTCCTCTGACTCAGCCAAAGACTTTGTCGAACAATATGTTTCTGAGTTATTAGAAACTCAGGGTTACGCGACAGCTGTTGACGTATTAGAGCATTTCTCTATTCACCAATCTGGGCAGTCCCTTCTCTATAGCATGTTGCAGAACAATGAATTTAAGGCAGCTGAGAAATGGGCCACTTTCATGGGAAAGCAAATGTTACATCTGCTTGTCCAGGAGTTGATAAATAGAAACAAACTAAAAAGTGCGTATGGCGTTATAAAGAAAAATGATTTACAGAAGGAATTTCCCGATGTCTATCAGAAGTGTAAAGAGAGGTACTTTGATGACCCCTATGCTGTATTTTTCTTCTTTGAACTCAAATTTTCATGTGCTCTTGGTATTGTTCTGTCTCCATTGGCTTAAAATCTTATGAATAATTATGATTCTTACTCAGCTCCTTGAAGAATTTAGCTGAAAAAGGATGCTGGGATGTTGCTGAGGCAAAAGCAAACAACAATAGGCAATTTCTTGAATATCTGGTTAGTATACTCCATAGATTCATTACTCATATTTTGAAGATTTTTAAGTAATACATATACTTCATTACAAGCAAAATGTCTTACCTGCTGAACCTGAGTTCTGGGTTTTTTGACAAATAGTTCTTAAAGCTCAAAGTCTTCATATCTTTACCTAACCTTTATGTGTTTTAAGGTTTACTTGGCATTGGAGGCTGGTTATTTCGAAAAAGTTGATGAACTTTGCACTAGGTACTCCCTCACGGGTTTTCTGAATATCAAAGGTATGCCATCTGTACCGTAGTCTCTTGCACTTAGAAGTTTCATTGTATATGTCGGGCCGTTTTTCCAGCTTTACCAAGATCAATATCTCATGTTAGACTATTATTATTCAGAAGTGTTCTACAAACTATCTTGTTGGCCTTTGTCCATTCCCTATTAGTTGGCCTTTGTCCATTCCCTATTACCCCTAGTCATTCCTTTTTACCTTTTATTCTTTATTGCCTCAAGTTTGTCCACTTTATTTGTTTTTTAATTGGATAAGAATGGATAGGCATCTCGCATAAAATTTTTCAAGTAGAAAATAAAGAGCTAAAAGGTGCGGCAAGAAAACAAGGAACTGGTTGAATGCCTCAGGGAATTGCTTCCATGGGCATTAAGAAGTGGAGTTGCCGGAGATAGTGAATAAACCAAAGAAATCCTTATCATTATTCCTAGGTTATTACGGCCTTTGTTAAGATACTGGCCATGTTCTTTTATTCCTCAAAAAAGTTAAATTGTGTTCAAAATAAGGGAAAGAAGGTTCTTGCTGATCAAAAAAGGGTAAAATGTAACCTAGTGAGCTACTCAATTTTACCATGGGCATTAAGAAGGGCCTTTTCCTTCTCCCGTTGATCACCCAATGAAGGAAGGTAGACTACTTGTCACCCTTAGTGAGCTACTCAATTTTACCTCTCTGTCTCGAGAAAGAGAAAATTAGTGTAAGTGTAAAATTAATATCATTTAGCAAAAACTAAAAAAGTGTAAAATTAATATCATTTAGCAAAAACTAATTTAGTATATTTTTAGCTTATTGACACATTATTGTATTAAAAACTAAGGAACAAAAATTATCTTCGACTTCCCTAATCTTCTAGGCAGTCATCAATTCAGTAGTAAATTATCTTTGAGTCACCTAGGATGATTTCCACTATCAGGAACAAATTTTGGTTTCTGTACATTATATTGCTTCAATGTTCTTGAACATTATCTACACAAAACATTAGCATGCTTTGAATCACTGCAGTTAAATTTATCAGCATATTGGTTTTCTGTAGAACGTGAGGGAAGTTATGAACAGAAGTTACCTAACCACTATCTGGATCTTAATCAACTAATTGATGGAAATATATTATGGATCGACAACGCTGATGCTTTACATCGTGCAACATGCCATATCGAGGAGTGTAAAGTTGTTGGTATTGATTGTGAGTGGAAACCTAACTATATAAAGGGCAAGAAACCGAACAAGGTAATTGCTGACAAATTATTTGTCCTCTCTGTTTGTGCTTTTCTCCTCCAATTCCTTTCTTATATTGTTGTATCTGCACTGGTTGTATTTCATTTTATTTTTTAATCTATTAAGAACGTTGAAGTACGTAGCAAATGCACCTGTTATTTTGGTTCTTTGGATTGAAAGAAACAATAGGGGTTTCAATAACAAAAACATAACAACAATAGTAATAAAATAAAATTGGACCATGTTATTTCTGTAGCCCTTTACTGTTATTTCTAATGTTTTACCTGTCTTGGTTTCATCTCAGGTATCTATAATGCAAATTGCTTCCGAGAAAATGGCTTTCATCTTTGACTTGATCAAGTTATATGATGATGTCCCTGACATTCTAGACAATTGCCTGACTCGCATCTTGCAGTCTTCTAGTATTTTGAAGCTCGGTTAGTAGTATCTGAATGTTTCAACTTCGTGTAACTCACTTGAAGTCTTGAATTTTCCATTCATATCCCAAAGTCTAGTTCTTAGTGATTGAATTGTTATGTCCTTTAAATTATTTTAATTTTTAATGTGAACCTGAAACATAATTTAAAGATGCCCAATCCCGATAGTATCTTCTTTTATATATTCATAAAGAATAACACTTCTTGATGTCTGAAAAAGTGCCTGTTTTACTGCTACCTTTCTATGTTCATGTACTAACTCCCTTTCTTTTCTGGGATTGCTAATTTTGAGTAAATATGTTCTTGTCTTTGTAGGCTACAATTTTTTATGCGATGTAAAGCAACTGTCCCATTCGTATGAATCATTGAAGTGTTTCAAACATTATGAGATGTTACTAGACATTCAAAATATATTTGACCATAGTGGAGGTCTATCTGGGCTTGCACAGGTAACATAACTGCAATTACACAAAACTCTCTTCTTCCTCGAATTTGATATTGAAATCATTTGGTTTTTTCCCATACTATTGCCTACTCCTCGCATAGCTCTTTTATCAATCAAAGCTTTATTGGCTTGTTTTTTTTTTTAATGTTGTAATTGTTTTAGAAAGTGTTGGGAGCTGGATTGAACAAGACACGAAGAAATAGTGACTGGGAACAGCGGCCTTTAACGGTGAATCAGGTCAATTTCTTTCTTGCTCAATTTTTTATATCAGTTTGACTTCTCTATAGATGAGGGGAGTGACAAAAGCAATAGCAAGCGTTTGAATAATGCACGTATCAGACCATTTTATTCAAGTTGGATAATATTTTGTGGTTTGGATCTTTGTCATTACTCATTACTATTGACAAAAGGTGAGAATGGGGTAAGCAGATTTGAATCCACATTCTGCAGTTTGTTTAAAATTTGAACTTAGTAGATACGTCACTGATAAATGGATATTTATTGAAAATTTTCCTAGTTCTGTAAAAAAAAAAAACCATGGATGATGCTCCTTTTTTTCATTTTTTTTTTCCTTTCTACGTTGTAGTAAGATATGCCTACGGATTTTGTAGAGGTGAAATTATTTCTTGCTCCCTGTTATTTCCCACTTACTAAACGGGTTTCTCTCATGTGTCTTTGCTTGTAGATTTTAAAATTTCTTTTAATTTCTCTTCCAAATAGACTAGAAAAGTTAAAAACATTTATTAAACTACAGTAGTATTGTTGAATGTTGAGCAGCTAGAATATGCAGCGCTGGATGCTGTGGTGCTCGTCCATATCTTCCAACATGTTCGAGATCAATCACAGCCATCTACGACAACAGAAGGGGAAACACGACTTGAGCGGAAATCCTTTATTGTAAGTCTAAGTCCTCTGTTCTTATATATACCCAGTTGACAAAATATGAAAATTTTGAATCCTAATTGAATCGGAGCAATTGACTAGCTATCGTCTTCTGTCGCTCATTCTTTCTAACTCCTTGGATAATAATGTAGGTCTCCCACATGGATAACTCGTCAAAACTGAAGAAGAAAAAAGAGAGGAGCGAAAAGGAAGCAGAAGTTGTAACCAAGTTGTAATGTCAGATGTGAATTATGTTAGGATGTTTATGGTACACTCGTGCCTTTTCTGTACATATTCATTCAAATCAAAATTGTATGTTGATCTTCACTGTACTTCAGTGATTTCCATGAAAATAGTAAATGTGTACAGTAAATCATGCTTATGTTAATATGGATTATGCACGAAACCTTCTGTTTAAATACTCTCATACTTATATCATTGCCTTTGGCACAGGAACAATGAGGAGTAAAGACTAGGAATTACTTTGTAGATTGGATCAATTTGTTCTCTTAGCGAGTGACAACAGATTTATATTATTAATCTTTGATCTTGGCGACTACTTGTGGATTTGAACGTTTGATGTCTAAGGATGGAGGTTATCAGTATTAGTAACAATATTTCTCATCGGTATTAGTGTTGTATTGATAACTTAATATCAGTAGACCATAATAAGTATCACTTTGAGAGTATGAGTATTGGAATTGGTAATTGGTATATTTCCTTCGCGTTTGAGGCTCATTTACAATCGAT

mRNA sequence

CAAAAGAAAAAAAAAAAAAGAAGAAAAAATTGATTTTGCCTAAATATTCAACCAATGTTTATTTTTTGTCCAACCTTCCTAGTTCCTCATAAGAAAGTTATCCAATGCAGAGTTTATCATGAAGACATTTTTTTGTCATACCATATTGGAGTTATTTCGGTTCATCTTCATTCTATTTATTTATATTCTACATTTTTAATGTCACCTAGTACAAATGCAGGCACATTGAAAGCAACTAAGAAGTTTCGATTCCTTCAGCAACAAGTTCATTTAGTACTTCATAATGGTCCCCAACCTGGACCAGCTACCTTTGTTATTCGTTGCCTATACGTCTTGCCTATATTTGGACTATATAGTGAAGGATTCAGCCACTTGATTACCTCAGCCCTCCAACGTTTTCTGAAAGTTGTGATAACTCCAGCAGACCTTGACGAAGCAAAGGACCTAGCTGCTCAGTTATTTATTGATATTGTTGGAGGATTCATTGCGCATGATGACAGGATAGTCGTGAAGATTATACAGATATTTGATGTTCAATTGTCCGATGTTGAGAAAGTTATGTTTGAATCTAAGGCAAGAAATAGGTGTTCCTCTGACTCAGCCAAAGACTTTGTCGAACAATATGTTTCTGAGTTATTAGAAACTCAGGGTTACGCGACAGCTGTTGACGTATTAGAGCATTTCTCTATTCACCAATCTGGGCAGTCCCTTCTCTATAGCATGTTGCAGAACAATGAATTTAAGGCAGCTGAGAAATGGGCCACTTTCATGGGAAAGCAAATGTTACATCTGCTTGTCCAGGAGTTGATAAATAGAAACAAACTAAAAAGTGCGTATGGCGTTATAAAGAAAAATGATTTACAGAAGGAATTTCCCGATGTCTATCAGAAGTGTAAAGAGAGCTCCTTGAAGAATTTAGCTGAAAAAGGATGCTGGGATGTTGCTGAGGCAAAAGCAAACAACAATAGGCAATTTCTTGAATATCTGGTTTACTTGGCATTGGAGGCTGGTTATTTCGAAAAAGTTGATGAACTTTGCACTAGGTACTCCCTCACGGGTTTTCTGAATATCAAAGAACGTGAGGGAAGTTATGAACAGAAGTTACCTAACCACTATCTGGATCTTAATCAACTAATTGATGGAAATATATTATGGATCGACAACGCTGATGCTTTACATCGTGCAACATGCCATATCGAGGAGTGTAAAGTTGTTGGTATTGATTGTGAGTGGAAACCTAACTATATAAAGGGCAAGAAACCGAACAAGGTATCTATAATGCAAATTGCTTCCGAGAAAATGGCTTTCATCTTTGACTTGATCAAGTTATATGATGATGTCCCTGACATTCTAGACAATTGCCTGACTCGCATCTTGCAGTCTTCTAGTATTTTGAAGCTCGGCTACAATTTTTTATGCGATGTAAAGCAACTGTCCCATTCGTATGAATCATTGAAGTGTTTCAAACATTATGAGATGTTACTAGACATTCAAAATATATTTGACCATAGTGGAGGTCTATCTGGGCTTGCACAGAAAGTGTTGGGAGCTGGATTGAACAAGACACGAAGAAATAGTGACTGGGAACAGCGGCCTTTAACGGTGAATCAGCTAGAATATGCAGCGCTGGATGCTGTGGTGCTCGTCCATATCTTCCAACATGTTCGAGATCAATCACAGCCATCTACGACAACAGAAGGGGAAACACGACTTGAGCGGAAATCCTTTATTGTCTCCCACATGGATAACTCGTCAAAACTGAAGAAGAAAAAAGAGAGGAGCGAAAAGGAAGCAGAAGTTGTAACCAAGTTGTAATGTCAGATGTGAATTATGTTAGGATGTTTATGGTACACTCGTGCCTTTTCTGTACATATTCATTCAAATCAAAATTGAACAATGAGGAGTAAAGACTAGGAATTACTTTGTAGATTGGATCAATTTGTTCTCTTAGCGAGTGACAACAGATTTATATTATTAATCTTTGATCTTGGCGACTACTTGTGGATTTGAACGTTTGATGTCTAAGGATGGAGGTTATCAGTATTAGTAACAATATTTCTCATCGGTATTAGTGTTGTATTGATAACTTAATATCAGTAGACCATAATAAGTATCACTTTGAGAGTATGAGTATTGGAATTGGTAATTGGTATATTTCCTTCGCGTTTGAGGCTCATTTACAATCGAT

Coding sequence (CDS)

ATGTTTATTTTTTGTCCAACCTTCCTAGTTCCTCATAAGAAAGTTATCCAATGCAGAGTTTATCATGAAGACATTTTTTTGTCATACCATATTGGAGTTATTTCGGTTCATCTTCATTCTATTTATTTATATTCTACATTTTTAATGTCACCTAGTACAAATGCAGGCACATTGAAAGCAACTAAGAAGTTTCGATTCCTTCAGCAACAAGTTCATTTAGTACTTCATAATGGTCCCCAACCTGGACCAGCTACCTTTGTTATTCGTTGCCTATACGTCTTGCCTATATTTGGACTATATAGTGAAGGATTCAGCCACTTGATTACCTCAGCCCTCCAACGTTTTCTGAAAGTTGTGATAACTCCAGCAGACCTTGACGAAGCAAAGGACCTAGCTGCTCAGTTATTTATTGATATTGTTGGAGGATTCATTGCGCATGATGACAGGATAGTCGTGAAGATTATACAGATATTTGATGTTCAATTGTCCGATGTTGAGAAAGTTATGTTTGAATCTAAGGCAAGAAATAGGTGTTCCTCTGACTCAGCCAAAGACTTTGTCGAACAATATGTTTCTGAGTTATTAGAAACTCAGGGTTACGCGACAGCTGTTGACGTATTAGAGCATTTCTCTATTCACCAATCTGGGCAGTCCCTTCTCTATAGCATGTTGCAGAACAATGAATTTAAGGCAGCTGAGAAATGGGCCACTTTCATGGGAAAGCAAATGTTACATCTGCTTGTCCAGGAGTTGATAAATAGAAACAAACTAAAAAGTGCGTATGGCGTTATAAAGAAAAATGATTTACAGAAGGAATTTCCCGATGTCTATCAGAAGTGTAAAGAGAGCTCCTTGAAGAATTTAGCTGAAAAAGGATGCTGGGATGTTGCTGAGGCAAAAGCAAACAACAATAGGCAATTTCTTGAATATCTGGTTTACTTGGCATTGGAGGCTGGTTATTTCGAAAAAGTTGATGAACTTTGCACTAGGTACTCCCTCACGGGTTTTCTGAATATCAAAGAACGTGAGGGAAGTTATGAACAGAAGTTACCTAACCACTATCTGGATCTTAATCAACTAATTGATGGAAATATATTATGGATCGACAACGCTGATGCTTTACATCGTGCAACATGCCATATCGAGGAGTGTAAAGTTGTTGGTATTGATTGTGAGTGGAAACCTAACTATATAAAGGGCAAGAAACCGAACAAGGTATCTATAATGCAAATTGCTTCCGAGAAAATGGCTTTCATCTTTGACTTGATCAAGTTATATGATGATGTCCCTGACATTCTAGACAATTGCCTGACTCGCATCTTGCAGTCTTCTAGTATTTTGAAGCTCGGCTACAATTTTTTATGCGATGTAAAGCAACTGTCCCATTCGTATGAATCATTGAAGTGTTTCAAACATTATGAGATGTTACTAGACATTCAAAATATATTTGACCATAGTGGAGGTCTATCTGGGCTTGCACAGAAAGTGTTGGGAGCTGGATTGAACAAGACACGAAGAAATAGTGACTGGGAACAGCGGCCTTTAACGGTGAATCAGCTAGAATATGCAGCGCTGGATGCTGTGGTGCTCGTCCATATCTTCCAACATGTTCGAGATCAATCACAGCCATCTACGACAACAGAAGGGGAAACACGACTTGAGCGGAAATCCTTTATTGTCTCCCACATGGATAACTCGTCAAAACTGAAGAAGAAAAAAGAGAGGAGCGAAAAGGAAGCAGAAGTTGTAACCAAGTTGTAA

Protein sequence

MFIFCPTFLVPHKKVIQCRVYHEDIFLSYHIGVISVHLHSIYLYSTFLMSPSTNAGTLKATKKFRFLQQQVHLVLHNGPQPGPATFVIRCLYVLPIFGLYSEGFSHLITSALQRFLKVVITPADLDEAKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQLSDVEKVMFESKARNRCSSDSAKDFVEQYVSELLETQGYATAVDVLEHFSIHQSGQSLLYSMLQNNEFKAAEKWATFMGKQMLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLKNLAEKGCWDVAEAKANNNRQFLEYLVYLALEAGYFEKVDELCTRYSLTGFLNIKEREGSYEQKLPNHYLDLNQLIDGNILWIDNADALHRATCHIEECKVVGIDCEWKPNYIKGKKPNKVSIMQIASEKMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYESLKCFKHYEMLLDIQNIFDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALDAVVLVHIFQHVRDQSQPSTTTEGETRLERKSFIVSHMDNSSKLKKKKERSEKEAEVVTKL*
Homology
BLAST of CsGy1G025230 vs. ExPASy Swiss-Prot
Match: Q8N9H8 (Exonuclease mut-7 homolog OS=Homo sapiens OX=9606 GN=EXD3 PE=1 SV=3)

HSP 1 Score: 80.1 bits (196), Expect = 9.2e-14
Identity = 56/178 (31.46%), Postives = 91/178 (51.12%), Query Frame = 0

Query: 379 CHIEECKVVGIDCEWKPNYIKGKKPNKVSIMQIASEKMAFIFDLIKLYD----DVPDILD 438
           CH    +VVG+D EW P ++ G +P + S++Q+A E   F+ D++ L             
Sbjct: 392 CH----QVVGVDVEWTPVFVAGGRP-RPSLLQVAVEGHVFLLDVLALSQPPTGQGAQAFS 451

Query: 439 NCLTRILQSSSILKLGYNFLCDVKQLSHSYESL-----KCFKHYEMLLDIQNIFDHS--- 498
             + ++L   SI KLGY  + D+++L  S  +L     +     ++LL  + +   S   
Sbjct: 452 RLVAQLLSDPSITKLGYGMVGDLQKLGTSCPALAHVEKQILGGMDLLLVHRQMRVASVPA 511

Query: 499 ---------GGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALDAVVLVHIFQ 536
                     GLS L Q+VLG  L+KT++ S+W++RPL   Q+ YAA DA  L+ + Q
Sbjct: 512 PAVDRARELRGLSLLVQQVLGTALDKTQQLSNWDRRPLCEEQVIYAAADAYCLLEVHQ 564

BLAST of CsGy1G025230 vs. ExPASy Swiss-Prot
Match: Q84LH3 (Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 8.0e-10
Identity = 50/162 (30.86%), Postives = 79/162 (48.77%), Query Frame = 0

Query: 387 VGIDCEWKPNYIKGKKPNKVSIMQIASEKMAFIFDLIKLYDDVPDI----LDNCLTRILQ 446
           VG+D EW+P++ KG  P KV+ +QI  +           Y DV  I    +   L  +++
Sbjct: 130 VGLDIEWRPSFRKGVLPGKVATVQICVDSN---------YCDVMHIFHSGIPQSLQHLIE 189

Query: 447 SSSILKLGYNFLCDVKQLSHSYESLKCFKHYEMLLDIQNI---FDHSGGLSGLAQKVLGA 506
            S+++K+G     D  +L H Y      K  E L D+ N     D   GL+ L + ++  
Sbjct: 190 DSTLVKVGIGIDGDSVKLFHDYG--VSIKDVEDLSDLANQKIGGDKKWGLASLTETLVCK 249

Query: 507 GLNKTR--RNSDWEQRPLTVNQLEYAALDAVVLVHIFQHVRD 540
            L K    R  +WE  PL+  QL+YAA DA    H+++ ++D
Sbjct: 250 ELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVLKD 280

BLAST of CsGy1G025230 vs. ExPASy Swiss-Prot
Match: P34607 (Exonuclease mut-7 OS=Caenorhabditis elegans OX=6239 GN=mut-7 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.1e-09
Identity = 45/173 (26.01%), Postives = 82/173 (47.40%), Query Frame = 0

Query: 387 VGIDCEWKPNYIKGKKPNKVSIMQIASEKMAFIFDLIKL--YDDVPDILDNCLTRILQSS 446
           VG D EWKP+ +     +K++I+Q+  +   ++ D ++L   +   D      +R+   S
Sbjct: 432 VGFDSEWKPSNLTAVHDSKIAIIQLFFKNCVWLVDCVELEKANMADDWWQKFASRLFGDS 491

Query: 447 SILKLGYNFLCDVKQLSHSYESLKCFKHYEMLLDIQNIFD-------------------- 506
            +  +G++   D+  ++    ++   K    + D +N FD                    
Sbjct: 492 PVKVVGFDMRNDLDAMA----TIPALKSSMKIEDTKNAFDLKRLAENVCDIDMEILELPK 551

Query: 507 HSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALDAVVLVHIFQHV 538
            +  L+ L   +LG  L+KT + S+W+ RPL   Q+ YAALDAVV+V  F+ +
Sbjct: 552 KTFKLADLTHYLLGLELDKTEQCSNWQCRPLRKKQIVYAALDAVVVVETFKKI 600

BLAST of CsGy1G025230 vs. ExPASy Swiss-Prot
Match: Q179T2 (Exonuclease mut-7 homolog OS=Aedes aegypti OX=7159 GN=AAEL005527 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.8e-09
Identity = 50/209 (23.92%), Postives = 92/209 (44.02%), Query Frame = 0

Query: 351 PNHYLDLNQLIDGNILWIDNADALHRATCHIEECKVVGIDCEWKPNYIKGKKPNKVSIMQ 410
           P H L L+   + ++  +D+ D  +     +    ++  D EWKP +      N+VS++Q
Sbjct: 426 PAHTLRLD---ESHVHLVDSKDKFYAMLSDLCRQSMIAFDSEWKPTF---GGANEVSLIQ 485

Query: 411 IASEKMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSH---SYESL 470
           +A+    ++ D++    +  D        +     +LKL +    D+        S+  +
Sbjct: 486 LATWDDVYMIDVMVSQLEPLD-WAALAKNVFNRDDVLKLSFAPSTDISMFQKALPSFNVM 545

Query: 471 KCFKHYEMLLDIQNIFDH----------------SGGLSGLAQKVLGAGLNKTRRNSDWE 530
              +    +LD+Q ++ H                +  L+ L +  LG  L+K+ + S+W 
Sbjct: 546 YSSQSTSAILDLQLLWRHVERFDSFRFPYHEESVNQNLANLVRLCLGKKLDKSNQFSNWA 605

Query: 531 QRPLTVNQLEYAALDAVVLVHIFQHVRDQ 541
           QRPL   QL YAALDA  L+ I+  +  Q
Sbjct: 606 QRPLRKEQLRYAALDAFCLLEIYDAIEKQ 627

BLAST of CsGy1G025230 vs. ExPASy Swiss-Prot
Match: Q9VIF1 (Exonuclease mut-7 homolog OS=Drosophila melanogaster OX=7227 GN=Nbr PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 3.1e-09
Identity = 67/307 (21.82%), Postives = 126/307 (41.04%), Query Frame = 0

Query: 295 DVAEAKANNNRQFLEYLVYLALEAGYFEKVD--------------ELCTRYSLTGFLNIK 354
           +V    A N+    +++ YLA    + E +               E+ T+ S  G     
Sbjct: 323 EVVSVHAFNHELRTDFVKYLASAGAHSEAIYWYTEFNIDPKDCPLEIETQVSQNGAGKAS 382

Query: 355 EREGSYEQKLPNHYLDL---NQLIDGNILWIDNADALHRATCHIEECKVVGIDCEWKPNY 414
             E   +++ P+   D+     L D  ++ ++ AD   R   H+++  V+ +D EW  + 
Sbjct: 383 GWESPGKERCPSSRCDMYLTMDLPDECLIIVNKADEFDRMLYHLQQECVIYLDSEWMQSV 442

Query: 415 IKGKKPNKVSIMQIASEKMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDV 474
                 N++ ++QIA+    ++ D +       +        I  + +I K+G++ + D+
Sbjct: 443 C---GDNQLCVLQIATGHNVYLIDCLARESLRSEHWRLLGANIFNNVNIRKVGFSMVSDL 502

Query: 475 K------------QLSHSYESLKCF------KHYEMLLDIQNIFDHSGGLSGLAQKVLGA 534
                        Q+ H Y  L+        + + + L   N+      L+ L+   LG 
Sbjct: 503 SVLQRSLPLQLRLQMPHHYLDLRNLWLELKKQRFGVELPFGNVNRAGDALTDLSLACLGK 562

Query: 535 GLNKTRRNSDWEQRPLTVNQLEYAALDAVVLVHIFQHVRDQSQPSTTTEGETRLERKSFI 567
            LNK+ + S+W  RPL   Q+ YAA+DA  L+ I+                T +ER SFI
Sbjct: 563 KLNKSNQCSNWANRPLRREQILYAAIDARCLMLIY---------------NTLIERVSFI 611

BLAST of CsGy1G025230 vs. NCBI nr
Match: XP_004135767.1 (uncharacterized protein LOC101205010 isoform X1 [Cucumis sativus] >XP_031736262.1 uncharacterized protein LOC101205010 isoform X1 [Cucumis sativus] >XP_031736263.1 uncharacterized protein LOC101205010 isoform X1 [Cucumis sativus] >KGN66018.1 hypothetical protein Csa_007185 [Cucumis sativus])

HSP 1 Score: 1055 bits (2728), Expect = 0.0
Identity = 530/540 (98.15%), Postives = 533/540 (98.70%), Query Frame = 0

Query: 47  FLMSPSTNAGTLKATKKFRFLQQQVHLVLHNGPQPGPATFVIRCLYVLPIFGLYSEGFSH 106
           +L+      GTLKATKKFRFLQQQVHLVLHNGPQPGPATFVIRCLYVLPIFGLYSEGFSH
Sbjct: 41  YLLKECYIRGTLKATKKFRFLQQQVHLVLHNGPQPGPATFVIRCLYVLPIFGLYSEGFSH 100

Query: 107 LITSALQRFLKVVITPADLDEAKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQLSDVE 166
           LITSALQRFLKVVITPADLDEAKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQLSDVE
Sbjct: 101 LITSALQRFLKVVITPADLDEAKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQLSDVE 160

Query: 167 KVMFESKARNRCSSDSAKDFVEQYVSELLETQGYATAVDVLEHFSIHQSGQSLLYSMLQN 226
           KVMFESKARNRCSSDSAKDFVEQYVSELLETQGYATAVDVLEHFSIHQSGQSLLY MLQN
Sbjct: 161 KVMFESKARNRCSSDSAKDFVEQYVSELLETQGYATAVDVLEHFSIHQSGQSLLYGMLQN 220

Query: 227 NEFKAAEKWATFMGKQMLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLK 286
           NEFKAAEKWATFMGKQMLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLK
Sbjct: 221 NEFKAAEKWATFMGKQMLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLK 280

Query: 287 NLAEKGCWDVAEAKANNNRQFLEYLVYLALEAGYFEKVDELCTRYSLTGFLNIKEREGSY 346
           NLAEKGCWDVAEAKANNNRQFLEYLVYLALEAGYFEKVDELCTRYSLTGFLNIKEREGSY
Sbjct: 281 NLAEKGCWDVAEAKANNNRQFLEYLVYLALEAGYFEKVDELCTRYSLTGFLNIKEREGSY 340

Query: 347 EQKLPNHYLDLNQLIDGNILWIDNADALHRATCHIEECKVVGIDCEWKPNYIKGKKPNKV 406
           EQKLPNHYLDLNQLIDGNILWIDNADALHRATCHIEECKVVGIDCEWKPNYIKGKKPNKV
Sbjct: 341 EQKLPNHYLDLNQLIDGNILWIDNADALHRATCHIEECKVVGIDCEWKPNYIKGKKPNKV 400

Query: 407 SIMQIASEKMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYES 466
           SIMQIASEKMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYES
Sbjct: 401 SIMQIASEKMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYES 460

Query: 467 LKCFKHYEMLLDIQNIFDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALD 526
           LKCFKHYEMLLDIQNIFDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALD
Sbjct: 461 LKCFKHYEMLLDIQNIFDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALD 520

Query: 527 AVVLVHIFQHVRDQSQPSTTTEGETRLERKSFIVSHMDNSSKLKKKKERSEKEAEVVTKL 586
           AVVLVHIFQHVRDQSQPSTTTEGETRLERKSFIVSHMDNSSKLKKKKERS+KEAEVVTKL
Sbjct: 521 AVVLVHIFQHVRDQSQPSTTTEGETRLERKSFIVSHMDNSSKLKKKKERSKKEAEVVTKL 580

BLAST of CsGy1G025230 vs. NCBI nr
Match: XP_031736264.1 (uncharacterized protein LOC101205010 isoform X2 [Cucumis sativus])

HSP 1 Score: 1053 bits (2722), Expect = 0.0
Identity = 529/533 (99.25%), Postives = 530/533 (99.44%), Query Frame = 0

Query: 54  NAGTLKATKKFRFLQQQVHLVLHNGPQPGPATFVIRCLYVLPIFGLYSEGFSHLITSALQ 113
           N  TLKATKKFRFLQQQVHLVLHNGPQPGPATFVIRCLYVLPIFGLYSEGFSHLITSALQ
Sbjct: 3   NYCTLKATKKFRFLQQQVHLVLHNGPQPGPATFVIRCLYVLPIFGLYSEGFSHLITSALQ 62

Query: 114 RFLKVVITPADLDEAKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQLSDVEKVMFESK 173
           RFLKVVITPADLDEAKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQLSDVEKVMFESK
Sbjct: 63  RFLKVVITPADLDEAKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQLSDVEKVMFESK 122

Query: 174 ARNRCSSDSAKDFVEQYVSELLETQGYATAVDVLEHFSIHQSGQSLLYSMLQNNEFKAAE 233
           ARNRCSSDSAKDFVEQYVSELLETQGYATAVDVLEHFSIHQSGQSLLY MLQNNEFKAAE
Sbjct: 123 ARNRCSSDSAKDFVEQYVSELLETQGYATAVDVLEHFSIHQSGQSLLYGMLQNNEFKAAE 182

Query: 234 KWATFMGKQMLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLKNLAEKGC 293
           KWATFMGKQMLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLKNLAEKGC
Sbjct: 183 KWATFMGKQMLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLKNLAEKGC 242

Query: 294 WDVAEAKANNNRQFLEYLVYLALEAGYFEKVDELCTRYSLTGFLNIKEREGSYEQKLPNH 353
           WDVAEAKANNNRQFLEYLVYLALEAGYFEKVDELCTRYSLTGFLNIKEREGSYEQKLPNH
Sbjct: 243 WDVAEAKANNNRQFLEYLVYLALEAGYFEKVDELCTRYSLTGFLNIKEREGSYEQKLPNH 302

Query: 354 YLDLNQLIDGNILWIDNADALHRATCHIEECKVVGIDCEWKPNYIKGKKPNKVSIMQIAS 413
           YLDLNQLIDGNILWIDNADALHRATCHIEECKVVGIDCEWKPNYIKGKKPNKVSIMQIAS
Sbjct: 303 YLDLNQLIDGNILWIDNADALHRATCHIEECKVVGIDCEWKPNYIKGKKPNKVSIMQIAS 362

Query: 414 EKMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYESLKCFKHY 473
           EKMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYESLKCFKHY
Sbjct: 363 EKMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYESLKCFKHY 422

Query: 474 EMLLDIQNIFDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALDAVVLVHI 533
           EMLLDIQNIFDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALDAVVLVHI
Sbjct: 423 EMLLDIQNIFDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALDAVVLVHI 482

Query: 534 FQHVRDQSQPSTTTEGETRLERKSFIVSHMDNSSKLKKKKERSEKEAEVVTKL 586
           FQHVRDQSQPSTTTEGETRLERKSFIVSHMDNSSKLKKKKERS+KEAEVVTKL
Sbjct: 483 FQHVRDQSQPSTTTEGETRLERKSFIVSHMDNSSKLKKKKERSKKEAEVVTKL 535

BLAST of CsGy1G025230 vs. NCBI nr
Match: XP_008450552.1 (PREDICTED: uncharacterized protein LOC103492118 [Cucumis melo] >XP_008450553.1 PREDICTED: uncharacterized protein LOC103492118 [Cucumis melo])

HSP 1 Score: 1009 bits (2608), Expect = 0.0
Identity = 506/540 (93.70%), Postives = 519/540 (96.11%), Query Frame = 0

Query: 47  FLMSPSTNAGTLKATKKFRFLQQQVHLVLHNGPQPGPATFVIRCLYVLPIFGLYSEGFSH 106
           +L+      GT+KATKKFRFLQQQVHLVLHNGPQPGP TFVI CLYVLPIFGLYSEGFSH
Sbjct: 41  YLLKECYIRGTVKATKKFRFLQQQVHLVLHNGPQPGPGTFVIHCLYVLPIFGLYSEGFSH 100

Query: 107 LITSALQRFLKVVITPADLDEAKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQLSDVE 166
           LITSALQRFLKVV TPADLD+AKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQL+DVE
Sbjct: 101 LITSALQRFLKVVTTPADLDKAKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQLTDVE 160

Query: 167 KVMFESKARNRCSSDSAKDFVEQYVSELLETQGYATAVDVLEHFSIHQSGQSLLYSMLQN 226
           KVMFESKARNRCSSDSAKDFVEQYVSEL+ETQGY TAVDVLEHFSIHQSGQSLLYSMLQN
Sbjct: 161 KVMFESKARNRCSSDSAKDFVEQYVSELIETQGYTTAVDVLEHFSIHQSGQSLLYSMLQN 220

Query: 227 NEFKAAEKWATFMGKQMLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLK 286
           NEFKAAEKWATFMGK MLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLK
Sbjct: 221 NEFKAAEKWATFMGKPMLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLK 280

Query: 287 NLAEKGCWDVAEAKANNNRQFLEYLVYLALEAGYFEKVDELCTRYSLTGFLNIKEREGSY 346
           NLAEKGCWDVAEAK N+NRQFLEYLVYLALEAGYFEKVDELC RYSLTGFLNIKEREGSY
Sbjct: 281 NLAEKGCWDVAEAKTNSNRQFLEYLVYLALEAGYFEKVDELCNRYSLTGFLNIKEREGSY 340

Query: 347 EQKLPNHYLDLNQLIDGNILWIDNADALHRATCHIEECKVVGIDCEWKPNYIKGKKPNKV 406
            QKL NHYL+LNQLI G+ILW+DNADALH AT HIEECKVVGIDCEWKPNYIKGKKPNKV
Sbjct: 341 GQKLTNHYLNLNQLIAGDILWVDNADALHHATRHIEECKVVGIDCEWKPNYIKGKKPNKV 400

Query: 407 SIMQIASEKMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYES 466
           SIMQI  E+MAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYES
Sbjct: 401 SIMQIGLEEMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYES 460

Query: 467 LKCFKHYEMLLDIQNIFDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALD 526
           LKCFKHYEMLLDIQN+FDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALD
Sbjct: 461 LKCFKHYEMLLDIQNVFDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALD 520

Query: 527 AVVLVHIFQHVRDQSQPSTTTEGETRLERKSFIVSHMDNSSKLKKKKERSEKEAEVVTKL 586
           AVVLVHIFQHVRDQSQPSTTTEGETRLE KSFIVSHMDNSSKLKKKKERS+KEAEVVTKL
Sbjct: 521 AVVLVHIFQHVRDQSQPSTTTEGETRLEWKSFIVSHMDNSSKLKKKKERSKKEAEVVTKL 580

BLAST of CsGy1G025230 vs. NCBI nr
Match: KAA0050943.1 (exonuclease mut-7-like protein isoform X1 [Cucumis melo var. makuwa] >TYK10290.1 exonuclease mut-7-like protein isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 976 bits (2522), Expect = 0.0
Identity = 485/513 (94.54%), Postives = 495/513 (96.49%), Query Frame = 0

Query: 49  MSPSTNAGTLKATKKFRFLQQQVHLVLHNGPQPGPATFVIRCLYVLPIFGLYSEGFSHLI 108
           MSPS N GT+KATKKFRFLQQQVHLVLHNGPQPGP TFVI CLYVLPIFGLYSEGFSHLI
Sbjct: 1   MSPSANVGTVKATKKFRFLQQQVHLVLHNGPQPGPGTFVIHCLYVLPIFGLYSEGFSHLI 60

Query: 109 TSALQRFLKVVITPADLDEAKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQLSDVEKV 168
           TSALQRFLKVV TPADLD+AKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQL+DVEKV
Sbjct: 61  TSALQRFLKVVTTPADLDKAKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQLTDVEKV 120

Query: 169 MFESKARNRCSSDSAKDFVEQYVSELLETQGYATAVDVLEHFSIHQSGQSLLYSMLQNNE 228
           MFESKARNRCSSDSAKDFVEQYVSEL+ETQGY TAVDVLEHFSIHQSGQSLLYSMLQNNE
Sbjct: 121 MFESKARNRCSSDSAKDFVEQYVSELIETQGYTTAVDVLEHFSIHQSGQSLLYSMLQNNE 180

Query: 229 FKAAEKWATFMGKQMLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLKNL 288
           FKAAEKWATFMGK MLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLKNL
Sbjct: 181 FKAAEKWATFMGKPMLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLKNL 240

Query: 289 AEKGCWDVAEAKANNNRQFLEYLVYLALEAGYFEKVDELCTRYSLTGFLNIKEREGSYEQ 348
           AEKGCWDVAEAK N+NRQFLEYLVYLALEAGYFEKVDELC RYSLTGFLNIKEREGSY Q
Sbjct: 241 AEKGCWDVAEAKTNSNRQFLEYLVYLALEAGYFEKVDELCNRYSLTGFLNIKEREGSYGQ 300

Query: 349 KLPNHYLDLNQLIDGNILWIDNADALHRATCHIEECKVVGIDCEWKPNYIKGKKPNKVSI 408
           KL NHYL+LNQLI G+ILW+DNADALH AT HIEECKVVGIDCEWKPNYIKGKKPNKVSI
Sbjct: 301 KLTNHYLNLNQLIAGDILWVDNADALHHATRHIEECKVVGIDCEWKPNYIKGKKPNKVSI 360

Query: 409 MQIASEKMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYESLK 468
           MQI  E+MAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYESLK
Sbjct: 361 MQIGLEEMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYESLK 420

Query: 469 CFKHYEMLLDIQNIFDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALDAV 528
           CFKHYEMLLDIQN+FDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALDAV
Sbjct: 421 CFKHYEMLLDIQNVFDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALDAV 480

Query: 529 VLVHIFQHVRDQSQPSTTTEGETRLERKSFIVS 561
           VLVHIFQHVRDQSQPSTTTEGETRLE KSFI S
Sbjct: 481 VLVHIFQHVRDQSQPSTTTEGETRLEWKSFIAS 513

BLAST of CsGy1G025230 vs. NCBI nr
Match: XP_038880060.1 (uncharacterized protein LOC120071757 isoform X1 [Benincasa hispida] >XP_038880061.1 uncharacterized protein LOC120071757 isoform X1 [Benincasa hispida])

HSP 1 Score: 965 bits (2494), Expect = 0.0
Identity = 479/540 (88.70%), Postives = 510/540 (94.44%), Query Frame = 0

Query: 47  FLMSPSTNAGTLKATKKFRFLQQQVHLVLHNGPQPGPATFVIRCLYVLPIFGLYSEGFSH 106
           +L+      GTLKATKKFRFLQQQVHLVLHNGP+PGPATFVI CLYVLPIFGLYSEGFSH
Sbjct: 84  YLLKECYIRGTLKATKKFRFLQQQVHLVLHNGPRPGPATFVIHCLYVLPIFGLYSEGFSH 143

Query: 107 LITSALQRFLKVVITPADLDEAKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQLSDVE 166
           LITSALQRFLKVV T ADLD+AK+LAAQLF+DIVGGFI HDDRIVVKI+Q++DVQL+++E
Sbjct: 144 LITSALQRFLKVVTTLADLDKAKNLAAQLFMDIVGGFIEHDDRIVVKILQVYDVQLTNIE 203

Query: 167 KVMFESKARNRCSSDSAKDFVEQYVSELLETQGYATAVDVLEHFSIHQSGQSLLYSMLQN 226
           KV+F+SKARNRCSSDSAKDFVEQYVSEL+ TQ YATAVDVLEHFSIHQSGQSLLYSMLQN
Sbjct: 204 KVLFKSKARNRCSSDSAKDFVEQYVSELIGTQAYATAVDVLEHFSIHQSGQSLLYSMLQN 263

Query: 227 NEFKAAEKWATFMGKQMLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLK 286
           NEFKAAEKWATFMGK ML LLVQELINRNKLKSAYG+IKKND QKEFPDVYQKCKESSLK
Sbjct: 264 NEFKAAEKWATFMGKPMLRLLVQELINRNKLKSAYGIIKKNDFQKEFPDVYQKCKESSLK 323

Query: 287 NLAEKGCWDVAEAKANNNRQFLEYLVYLALEAGYFEKVDELCTRYSLTGFLNIKEREGSY 346
           NLAEKGCWDVAEAK N+NRQFLEYLVYLALEAGYFEKVDELC RYSLTGFLNIKEREG Y
Sbjct: 324 NLAEKGCWDVAEAKTNSNRQFLEYLVYLALEAGYFEKVDELCNRYSLTGFLNIKEREGGY 383

Query: 347 EQKLPNHYLDLNQLIDGNILWIDNADALHRATCHIEECKVVGIDCEWKPNYIKGKKPNKV 406
            QK  NHYL+LNQLI G+ILW+DNADAL  ATCH+EECKVVGIDCEWKPNYIKGKKPNKV
Sbjct: 384 GQKSQNHYLNLNQLIVGDILWVDNADALRHATCHLEECKVVGIDCEWKPNYIKGKKPNKV 443

Query: 407 SIMQIASEKMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYES 466
           SIMQIASE+MAFIFDLIKLYDD+PDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYES
Sbjct: 444 SIMQIASEEMAFIFDLIKLYDDIPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYES 503

Query: 467 LKCFKHYEMLLDIQNIFDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALD 526
           L+CFKHYEMLLDIQN+FDHSGGLSGLA+KVLGAGLNKTRRNSDWEQRPLTVNQLE AALD
Sbjct: 504 LQCFKHYEMLLDIQNVFDHSGGLSGLAKKVLGAGLNKTRRNSDWEQRPLTVNQLENAALD 563

Query: 527 AVVLVHIFQHVRDQSQPSTTTEGETRLERKSFIVSHMDNSSKLKKKKERSEKEAEVVTKL 586
           AVVLVHIF+HVRDQSQPSTTTEG+TR E KSFIVSHMDN+ KLKKKK+RS+KE EVVT L
Sbjct: 564 AVVLVHIFRHVRDQSQPSTTTEGQTRPEWKSFIVSHMDNTLKLKKKKDRSKKEPEVVTNL 623

BLAST of CsGy1G025230 vs. ExPASy TrEMBL
Match: A0A0A0LWB0 (3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G561980 PE=4 SV=1)

HSP 1 Score: 1055 bits (2728), Expect = 0.0
Identity = 530/540 (98.15%), Postives = 533/540 (98.70%), Query Frame = 0

Query: 47  FLMSPSTNAGTLKATKKFRFLQQQVHLVLHNGPQPGPATFVIRCLYVLPIFGLYSEGFSH 106
           +L+      GTLKATKKFRFLQQQVHLVLHNGPQPGPATFVIRCLYVLPIFGLYSEGFSH
Sbjct: 41  YLLKECYIRGTLKATKKFRFLQQQVHLVLHNGPQPGPATFVIRCLYVLPIFGLYSEGFSH 100

Query: 107 LITSALQRFLKVVITPADLDEAKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQLSDVE 166
           LITSALQRFLKVVITPADLDEAKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQLSDVE
Sbjct: 101 LITSALQRFLKVVITPADLDEAKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQLSDVE 160

Query: 167 KVMFESKARNRCSSDSAKDFVEQYVSELLETQGYATAVDVLEHFSIHQSGQSLLYSMLQN 226
           KVMFESKARNRCSSDSAKDFVEQYVSELLETQGYATAVDVLEHFSIHQSGQSLLY MLQN
Sbjct: 161 KVMFESKARNRCSSDSAKDFVEQYVSELLETQGYATAVDVLEHFSIHQSGQSLLYGMLQN 220

Query: 227 NEFKAAEKWATFMGKQMLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLK 286
           NEFKAAEKWATFMGKQMLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLK
Sbjct: 221 NEFKAAEKWATFMGKQMLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLK 280

Query: 287 NLAEKGCWDVAEAKANNNRQFLEYLVYLALEAGYFEKVDELCTRYSLTGFLNIKEREGSY 346
           NLAEKGCWDVAEAKANNNRQFLEYLVYLALEAGYFEKVDELCTRYSLTGFLNIKEREGSY
Sbjct: 281 NLAEKGCWDVAEAKANNNRQFLEYLVYLALEAGYFEKVDELCTRYSLTGFLNIKEREGSY 340

Query: 347 EQKLPNHYLDLNQLIDGNILWIDNADALHRATCHIEECKVVGIDCEWKPNYIKGKKPNKV 406
           EQKLPNHYLDLNQLIDGNILWIDNADALHRATCHIEECKVVGIDCEWKPNYIKGKKPNKV
Sbjct: 341 EQKLPNHYLDLNQLIDGNILWIDNADALHRATCHIEECKVVGIDCEWKPNYIKGKKPNKV 400

Query: 407 SIMQIASEKMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYES 466
           SIMQIASEKMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYES
Sbjct: 401 SIMQIASEKMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYES 460

Query: 467 LKCFKHYEMLLDIQNIFDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALD 526
           LKCFKHYEMLLDIQNIFDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALD
Sbjct: 461 LKCFKHYEMLLDIQNIFDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALD 520

Query: 527 AVVLVHIFQHVRDQSQPSTTTEGETRLERKSFIVSHMDNSSKLKKKKERSEKEAEVVTKL 586
           AVVLVHIFQHVRDQSQPSTTTEGETRLERKSFIVSHMDNSSKLKKKKERS+KEAEVVTKL
Sbjct: 521 AVVLVHIFQHVRDQSQPSTTTEGETRLERKSFIVSHMDNSSKLKKKKERSKKEAEVVTKL 580

BLAST of CsGy1G025230 vs. ExPASy TrEMBL
Match: A0A1S3BPE9 (uncharacterized protein LOC103492118 OS=Cucumis melo OX=3656 GN=LOC103492118 PE=4 SV=1)

HSP 1 Score: 1009 bits (2608), Expect = 0.0
Identity = 506/540 (93.70%), Postives = 519/540 (96.11%), Query Frame = 0

Query: 47  FLMSPSTNAGTLKATKKFRFLQQQVHLVLHNGPQPGPATFVIRCLYVLPIFGLYSEGFSH 106
           +L+      GT+KATKKFRFLQQQVHLVLHNGPQPGP TFVI CLYVLPIFGLYSEGFSH
Sbjct: 41  YLLKECYIRGTVKATKKFRFLQQQVHLVLHNGPQPGPGTFVIHCLYVLPIFGLYSEGFSH 100

Query: 107 LITSALQRFLKVVITPADLDEAKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQLSDVE 166
           LITSALQRFLKVV TPADLD+AKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQL+DVE
Sbjct: 101 LITSALQRFLKVVTTPADLDKAKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQLTDVE 160

Query: 167 KVMFESKARNRCSSDSAKDFVEQYVSELLETQGYATAVDVLEHFSIHQSGQSLLYSMLQN 226
           KVMFESKARNRCSSDSAKDFVEQYVSEL+ETQGY TAVDVLEHFSIHQSGQSLLYSMLQN
Sbjct: 161 KVMFESKARNRCSSDSAKDFVEQYVSELIETQGYTTAVDVLEHFSIHQSGQSLLYSMLQN 220

Query: 227 NEFKAAEKWATFMGKQMLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLK 286
           NEFKAAEKWATFMGK MLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLK
Sbjct: 221 NEFKAAEKWATFMGKPMLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLK 280

Query: 287 NLAEKGCWDVAEAKANNNRQFLEYLVYLALEAGYFEKVDELCTRYSLTGFLNIKEREGSY 346
           NLAEKGCWDVAEAK N+NRQFLEYLVYLALEAGYFEKVDELC RYSLTGFLNIKEREGSY
Sbjct: 281 NLAEKGCWDVAEAKTNSNRQFLEYLVYLALEAGYFEKVDELCNRYSLTGFLNIKEREGSY 340

Query: 347 EQKLPNHYLDLNQLIDGNILWIDNADALHRATCHIEECKVVGIDCEWKPNYIKGKKPNKV 406
            QKL NHYL+LNQLI G+ILW+DNADALH AT HIEECKVVGIDCEWKPNYIKGKKPNKV
Sbjct: 341 GQKLTNHYLNLNQLIAGDILWVDNADALHHATRHIEECKVVGIDCEWKPNYIKGKKPNKV 400

Query: 407 SIMQIASEKMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYES 466
           SIMQI  E+MAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYES
Sbjct: 401 SIMQIGLEEMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYES 460

Query: 467 LKCFKHYEMLLDIQNIFDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALD 526
           LKCFKHYEMLLDIQN+FDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALD
Sbjct: 461 LKCFKHYEMLLDIQNVFDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALD 520

Query: 527 AVVLVHIFQHVRDQSQPSTTTEGETRLERKSFIVSHMDNSSKLKKKKERSEKEAEVVTKL 586
           AVVLVHIFQHVRDQSQPSTTTEGETRLE KSFIVSHMDNSSKLKKKKERS+KEAEVVTKL
Sbjct: 521 AVVLVHIFQHVRDQSQPSTTTEGETRLEWKSFIVSHMDNSSKLKKKKERSKKEAEVVTKL 580

BLAST of CsGy1G025230 vs. ExPASy TrEMBL
Match: A0A5D3CG08 (Exonuclease mut-7-like protein isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004410 PE=4 SV=1)

HSP 1 Score: 976 bits (2522), Expect = 0.0
Identity = 485/513 (94.54%), Postives = 495/513 (96.49%), Query Frame = 0

Query: 49  MSPSTNAGTLKATKKFRFLQQQVHLVLHNGPQPGPATFVIRCLYVLPIFGLYSEGFSHLI 108
           MSPS N GT+KATKKFRFLQQQVHLVLHNGPQPGP TFVI CLYVLPIFGLYSEGFSHLI
Sbjct: 1   MSPSANVGTVKATKKFRFLQQQVHLVLHNGPQPGPGTFVIHCLYVLPIFGLYSEGFSHLI 60

Query: 109 TSALQRFLKVVITPADLDEAKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQLSDVEKV 168
           TSALQRFLKVV TPADLD+AKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQL+DVEKV
Sbjct: 61  TSALQRFLKVVTTPADLDKAKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQLTDVEKV 120

Query: 169 MFESKARNRCSSDSAKDFVEQYVSELLETQGYATAVDVLEHFSIHQSGQSLLYSMLQNNE 228
           MFESKARNRCSSDSAKDFVEQYVSEL+ETQGY TAVDVLEHFSIHQSGQSLLYSMLQNNE
Sbjct: 121 MFESKARNRCSSDSAKDFVEQYVSELIETQGYTTAVDVLEHFSIHQSGQSLLYSMLQNNE 180

Query: 229 FKAAEKWATFMGKQMLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLKNL 288
           FKAAEKWATFMGK MLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLKNL
Sbjct: 181 FKAAEKWATFMGKPMLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLKNL 240

Query: 289 AEKGCWDVAEAKANNNRQFLEYLVYLALEAGYFEKVDELCTRYSLTGFLNIKEREGSYEQ 348
           AEKGCWDVAEAK N+NRQFLEYLVYLALEAGYFEKVDELC RYSLTGFLNIKEREGSY Q
Sbjct: 241 AEKGCWDVAEAKTNSNRQFLEYLVYLALEAGYFEKVDELCNRYSLTGFLNIKEREGSYGQ 300

Query: 349 KLPNHYLDLNQLIDGNILWIDNADALHRATCHIEECKVVGIDCEWKPNYIKGKKPNKVSI 408
           KL NHYL+LNQLI G+ILW+DNADALH AT HIEECKVVGIDCEWKPNYIKGKKPNKVSI
Sbjct: 301 KLTNHYLNLNQLIAGDILWVDNADALHHATRHIEECKVVGIDCEWKPNYIKGKKPNKVSI 360

Query: 409 MQIASEKMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYESLK 468
           MQI  E+MAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYESLK
Sbjct: 361 MQIGLEEMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYESLK 420

Query: 469 CFKHYEMLLDIQNIFDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALDAV 528
           CFKHYEMLLDIQN+FDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALDAV
Sbjct: 421 CFKHYEMLLDIQNVFDHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALDAV 480

Query: 529 VLVHIFQHVRDQSQPSTTTEGETRLERKSFIVS 561
           VLVHIFQHVRDQSQPSTTTEGETRLE KSFI S
Sbjct: 481 VLVHIFQHVRDQSQPSTTTEGETRLEWKSFIAS 513

BLAST of CsGy1G025230 vs. ExPASy TrEMBL
Match: A0A6J1HXY3 (uncharacterized protein LOC111467860 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467860 PE=4 SV=1)

HSP 1 Score: 893 bits (2307), Expect = 0.0
Identity = 446/541 (82.44%), Postives = 486/541 (89.83%), Query Frame = 0

Query: 47  FLMSPSTNAGTLKATKKFRFLQQQVHLVLHNGPQPGPATFVIRCLYVLPIFGLYSEGFSH 106
           +L+      GTLKATKKFR LQQQVHLVLHNGPQPGPA FVI CLYVLPIFGLYSEGFSH
Sbjct: 41  YLLKECYIRGTLKATKKFRSLQQQVHLVLHNGPQPGPAIFVIHCLYVLPIFGLYSEGFSH 100

Query: 107 LITSALQRFLKVVITPADLDEAKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQLSDVE 166
           LITSA QRFLKVV T  DLD+AK+LAAQLF+DIVGGFI HD+RIVVKI+Q+FD+QL+++E
Sbjct: 101 LITSAFQRFLKVVTTLTDLDKAKNLAAQLFVDIVGGFIKHDERIVVKILQVFDIQLTNIE 160

Query: 167 KVMFESKARNRCSSDSAKDFVEQYVSELLETQGYATAVDVLEHFSIHQSGQSLLYSMLQN 226
           KV F+SKARN C S SAKDF+EQYVSEL+ETQ Y TAVDVLEHFSI QSGQSLLYSMLQN
Sbjct: 161 KVRFDSKARNGCCSSSAKDFIEQYVSELVETQSYTTAVDVLEHFSIRQSGQSLLYSMLQN 220

Query: 227 NEFKAAEKWATFMGKQMLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLK 286
           NEFKAAEKWATFMG+ ML LLVQEL+NRNK+KSAYG++K N LQ EFPDVYQKCKESSLK
Sbjct: 221 NEFKAAEKWATFMGRPMLCLLVQELMNRNKVKSAYGIVKTNGLQTEFPDVYQKCKESSLK 280

Query: 287 NLAEKGCWDVAEAKANNNRQFLEYLVYLALEAGYFEKVDELCTRYSLTGFLNIKEREGSY 346
           NLAEKGCWDVAEAK  +NRQFLEYLVYLALEAGYFEKVDELC RYSLTGFLNIKEREG+Y
Sbjct: 281 NLAEKGCWDVAEAKTKSNRQFLEYLVYLALEAGYFEKVDELCNRYSLTGFLNIKEREGAY 340

Query: 347 EQKLPNHYLDLNQLIDGNILWIDNADALHRATCHIEECKVVGIDCEWKPNYIKGKKPNKV 406
            Q   NHYL+LNQLI G+ILW+DNADALH A CHIEECKV+GIDCEWKPNYIKG+KPNKV
Sbjct: 341 GQ---NHYLNLNQLITGDILWVDNADALHNAICHIEECKVIGIDCEWKPNYIKGRKPNKV 400

Query: 407 SIMQIASEKMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYES 466
           SIMQIASE+ AFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNF CD+KQLSHSY S
Sbjct: 401 SIMQIASEEKAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFQCDIKQLSHSYGS 460

Query: 467 LKCFKHYEMLLDIQNIF-DHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAAL 526
           L+CFKHY+MLLDIQN+F DH+GGLSGLA+KVLGAGLNKTRRNSDWEQRPLTVNQLEYAAL
Sbjct: 461 LECFKHYDMLLDIQNVFKDHTGGLSGLAKKVLGAGLNKTRRNSDWEQRPLTVNQLEYAAL 520

Query: 527 DAVVLVHIFQHVRDQSQPSTTTEGETRLERKSFIVSHMDNSSKLKKKKERSEKEAEVVTK 586
           DAVVLVHIF+HV DQSQPSTT E + RLE KS IVSHMDN  K ++KK RS  E +V T 
Sbjct: 521 DAVVLVHIFRHVGDQSQPSTTAEAQKRLEWKSIIVSHMDNPPK-QRKKNRSNMEPKVATN 577

BLAST of CsGy1G025230 vs. ExPASy TrEMBL
Match: A0A6J1HC93 (uncharacterized protein LOC111462125 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462125 PE=4 SV=1)

HSP 1 Score: 890 bits (2301), Expect = 0.0
Identity = 445/541 (82.26%), Postives = 485/541 (89.65%), Query Frame = 0

Query: 47  FLMSPSTNAGTLKATKKFRFLQQQVHLVLHNGPQPGPATFVIRCLYVLPIFGLYSEGFSH 106
           +L+      GTLKATKKFR LQQQVHLVLHNGPQPGPATFVI CLYVLPIFGLYSEGFSH
Sbjct: 41  YLLKECYIRGTLKATKKFRSLQQQVHLVLHNGPQPGPATFVIHCLYVLPIFGLYSEGFSH 100

Query: 107 LITSALQRFLKVVITPADLDEAKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQLSDVE 166
           LITSA QRFLKVV T  DLD+AK+LAAQLF+DIVGGFI HD+RI VKI+Q+FD+QL+++E
Sbjct: 101 LITSAFQRFLKVVTTLTDLDKAKNLAAQLFVDIVGGFIKHDERIAVKILQVFDIQLTNIE 160

Query: 167 KVMFESKARNRCSSDSAKDFVEQYVSELLETQGYATAVDVLEHFSIHQSGQSLLYSMLQN 226
           KV F+SKARN C S SAKDFVEQYVS+L+ETQ Y TAVDVLEHFSI QSGQSLLYSMLQN
Sbjct: 161 KVRFDSKARNGCCSSSAKDFVEQYVSQLVETQSYTTAVDVLEHFSIRQSGQSLLYSMLQN 220

Query: 227 NEFKAAEKWATFMGKQMLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKESSLK 286
           NEFKAAEKWATFMG+ ML LLVQEL+NRNK+KSAYG++K N LQ EFPDVYQKCKESSLK
Sbjct: 221 NEFKAAEKWATFMGRPMLCLLVQELMNRNKVKSAYGIVKTNGLQTEFPDVYQKCKESSLK 280

Query: 287 NLAEKGCWDVAEAKANNNRQFLEYLVYLALEAGYFEKVDELCTRYSLTGFLNIKEREGSY 346
           NLAEKGCWDVAEAK  +NRQFLEYLVYLALEAGYFEKVDELC RYSLTGFLNIKEREG+Y
Sbjct: 281 NLAEKGCWDVAEAKTKSNRQFLEYLVYLALEAGYFEKVDELCNRYSLTGFLNIKEREGAY 340

Query: 347 EQKLPNHYLDLNQLIDGNILWIDNADALHRATCHIEECKVVGIDCEWKPNYIKGKKPNKV 406
            Q   NHYL+LNQLI G+ILW+D ADALH A CHIEECKV+GIDCEWKPNYIKG+KPNKV
Sbjct: 341 GQ---NHYLNLNQLITGDILWVDKADALHNAVCHIEECKVIGIDCEWKPNYIKGRKPNKV 400

Query: 407 SIMQIASEKMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHSYES 466
           SIMQIASE+ AFIFDLIKLY+DVPDILDNCLTRILQSSSILKLGYNF CD+KQLSHSY S
Sbjct: 401 SIMQIASEEKAFIFDLIKLYNDVPDILDNCLTRILQSSSILKLGYNFQCDIKQLSHSYGS 460

Query: 467 LKCFKHYEMLLDIQNIF-DHSGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAAL 526
           L+CFKHY+MLLDIQN+F DH+GGLSGLA+KVLGAGLNKTRRNSDWEQRPLTVNQLEYAAL
Sbjct: 461 LECFKHYDMLLDIQNVFKDHTGGLSGLAKKVLGAGLNKTRRNSDWEQRPLTVNQLEYAAL 520

Query: 527 DAVVLVHIFQHVRDQSQPSTTTEGETRLERKSFIVSHMDNSSKLKKKKERSEKEAEVVTK 586
           DAVVLVHIF+HV DQSQPSTT E + RLE KSFIVSHMDN  K  +KK RS  E +V T 
Sbjct: 521 DAVVLVHIFRHVGDQSQPSTTAEAQKRLEWKSFIVSHMDNPPK-PRKKNRSNMEPKVATN 577

BLAST of CsGy1G025230 vs. TAIR 10
Match: AT1G56310.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 589.7 bits (1519), Expect = 2.5e-168
Identity = 296/534 (55.43%), Postives = 388/534 (72.66%), Query Frame = 0

Query: 47  FLMSPSTNAGTLKATKKFRFLQQQVHLVLHNGPQPGPATFVIRCLYVLPIFGLYSEGFSH 106
           +L+      G+LKATKKF+ LQ QVH VL N PQPGPATF+I CL +LP+FG+Y EGFSH
Sbjct: 41  YLLKECYKHGSLKATKKFQALQYQVHRVLANKPQPGPATFIINCLTLLPLFGVYGEGFSH 100

Query: 107 LITSALQRFLKVVITPA---DLDEAKDLAAQLFIDIVGGFIAHDDRIVVKIIQIFDVQLS 166
           L+ SAL+RF K V  P    D+  A+ LAAQ F+  VGG + +D++++V  +++FDV+L+
Sbjct: 101 LVISALRRFFKTVSEPTSEEDICLARKLAAQFFLATVGGSLTYDEKVMVHTLRVFDVRLT 160

Query: 167 DVEKVMFESKARNRCSSDSAKDFVEQYVSELLETQGYATAVDVLEHFSIHQSGQSLLYSM 226
            +++ +  S+   R        F+EQY+S+L++++ + TAV +LEHFS    G++ L  M
Sbjct: 161 SIDEALSISEVWQRYGFACGNAFLEQYISDLIKSKSFMTAVTLLEHFSFRFPGETFLQQM 220

Query: 227 LQNNEFKAAEKWATFMGKQMLHLLVQELINRNKLKSAYGVIKKNDLQKEFPDVYQKCKES 286
           +++  F+AAE+WATFMG+  L +LVQE  +RN LK AY +I KN LQ +FP++Y KCKES
Sbjct: 221 VEDKNFQAAERWATFMGRPSLCILVQEYGSRNMLKQAYNIINKNYLQHDFPELYHKCKES 280

Query: 287 SLKNLAEKGCWDVAEAKANNNRQFLEYLVYLALEAGYFEKVDELCTRYSLTGFLNIKERE 346
           +LK LAEK CWDVAE K   +RQ L+YLVYLA+EAGY EKVDELC RYSL G    +E E
Sbjct: 281 ALKVLAEKACWDVAEIKTKGDRQLLKYLVYLAVEAGYLEKVDELCDRYSLQGLPKAREAE 340

Query: 347 GSYEQKLPNHYLDLNQLIDGNILWIDNADALHRATCHIEECKVVGIDCEWKPNYIKGKKP 406
            ++ +K    +L LN L   +++W+D  + L +AT  +E C+VVGIDCEWKPNYIKG K 
Sbjct: 341 VAFVEK---SFLRLNDLAVEDVVWVDEVNELRKATSFLEGCRVVGIDCEWKPNYIKGSKQ 400

Query: 407 NKVSIMQIASEKMAFIFDLIKLYDDVPDILDNCLTRILQSSSILKLGYNFLCDVKQLSHS 466
           NKVSIMQI S+   FI DLIKLY+D  +ILDNCL+ ILQS S LKLGYNF CD+KQL+ S
Sbjct: 401 NKVSIMQIGSDTKIFILDLIKLYNDASEILDNCLSHILQSKSTLKLGYNFQCDIKQLALS 460

Query: 467 YESLKCFKHYEMLLDIQNIFDHS-GGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEY 526
           Y  LKCF+ Y+MLLDIQN+F+   GGL+GL +K+LG  LNKTRRNSDWEQRPL+ NQLEY
Sbjct: 461 YGDLKCFERYDMLLDIQNVFNEPFGGLAGLTKKILGVSLNKTRRNSDWEQRPLSQNQLEY 520

Query: 527 AALDAVVLVHIFQHVRDQSQPSTTTEGETRLERKSFIVSHMDNSSKLKKKKERS 577
           AALDA VL+HIF+HVRD     +++E     + KS IVSHM++  KLKKK  +S
Sbjct: 521 AALDAAVLIHIFRHVRDHPPHDSSSE---TTQWKSHIVSHMESPKKLKKKTPKS 568

BLAST of CsGy1G025230 vs. TAIR 10
Match: AT5G24340.1 (3'-5' exonuclease domain-containing protein )

HSP 1 Score: 69.3 bits (168), Expect = 1.2e-11
Identity = 48/172 (27.91%), Postives = 82/172 (47.67%), Query Frame = 0

Query: 386 VVGIDCEWKPNYIKGKKPNKVSIMQIASE-------KMAFIFDLIKLYDDVPDILDNCLT 445
           ++ +D EWKP +        V+++Q+A            F+ DL  ++  +P + +  L 
Sbjct: 30  IIALDAEWKPQHSNTSSFPTVTLLQVACRLSHATDVSDVFLIDLSSIH--LPSVWE-LLN 89

Query: 446 RILQSSSILKLGYNFLCDVKQLSHSYESLKC---FKHYEMLLDIQNIFDH---------- 505
            +  S  +LKLG+ F  D+  LS ++    C   F+  +  LDI +I+++          
Sbjct: 90  DMFVSPDVLKLGFRFKQDLVYLSSTFTQHGCEGGFQEVKQYLDITSIYNYLQHKRFGRKA 149

Query: 506 ---SGGLSGLAQKVLGAGLNKTRRNSDWEQRPLTVNQLEYAALDAVVLVHIF 535
                 L+ + +++L   L+K  + SDW  RPLT  Q  YAA DA  L+ IF
Sbjct: 150 PKDIKSLAAICKEMLDISLSKELQCSDWSYRPLTEEQKLYAATDAHCLLQIF 198

BLAST of CsGy1G025230 vs. TAIR 10
Match: AT4G13870.2 (Werner syndrome-like exonuclease )

HSP 1 Score: 67.0 bits (162), Expect = 5.7e-11
Identity = 50/162 (30.86%), Postives = 79/162 (48.77%), Query Frame = 0

Query: 387 VGIDCEWKPNYIKGKKPNKVSIMQIASEKMAFIFDLIKLYDDVPDI----LDNCLTRILQ 446
           VG+D EW+P++ KG  P KV+ +QI  +           Y DV  I    +   L  +++
Sbjct: 130 VGLDIEWRPSFRKGVLPGKVATVQICVDSN---------YCDVMHIFHSGIPQSLQHLIE 189

Query: 447 SSSILKLGYNFLCDVKQLSHSYESLKCFKHYEMLLDIQNI---FDHSGGLSGLAQKVLGA 506
            S+++K+G     D  +L H Y      K  E L D+ N     D   GL+ L + ++  
Sbjct: 190 DSTLVKVGIGIDGDSVKLFHDYG--VSIKDVEDLSDLANQKIGGDKKWGLASLTETLVCK 249

Query: 507 GLNKTR--RNSDWEQRPLTVNQLEYAALDAVVLVHIFQHVRD 540
            L K    R  +WE  PL+  QL+YAA DA    H+++ ++D
Sbjct: 250 ELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVLKD 280

BLAST of CsGy1G025230 vs. TAIR 10
Match: AT4G13870.1 (Werner syndrome-like exonuclease )

HSP 1 Score: 66.2 bits (160), Expect = 9.8e-11
Identity = 50/162 (30.86%), Postives = 78/162 (48.15%), Query Frame = 0

Query: 387 VGIDCEWKPNYIKGKKPNKVSIMQIASEKMAFIFDLIKLYDDVPDI----LDNCLTRILQ 446
           VG+D EW+P++ KG  P KV+ +QI  +           Y DV  I    +   L  +++
Sbjct: 130 VGLDIEWRPSFRKGVLPGKVATVQICVDSN---------YCDVMHIFHSGIPQSLQHLIE 189

Query: 447 SSSILKLGYNFLCDVKQLSHSYESLKCFKHYEMLLDIQNI---FDHSGGLSGLAQKVLGA 506
            S+++K+G     D  +L H Y      K  E L D+ N     D   GL+ L + ++  
Sbjct: 190 DSTLVKVGIGIDGDSVKLFHDYG--VSIKDVEDLSDLANQKIGGDKKWGLASLTETLVCK 249

Query: 507 GLNKTR--RNSDWEQRPLTVNQLEYAALDAVVLVHIFQHVRD 540
            L K    R  +WE  PL+  QL+YAA DA    H+++ + D
Sbjct: 250 ELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKDLPD 280

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8N9H89.2e-1431.46Exonuclease mut-7 homolog OS=Homo sapiens OX=9606 GN=EXD3 PE=1 SV=3[more]
Q84LH38.0e-1030.86Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=... [more]
P346071.1e-0926.01Exonuclease mut-7 OS=Caenorhabditis elegans OX=6239 GN=mut-7 PE=1 SV=1[more]
Q179T21.8e-0923.92Exonuclease mut-7 homolog OS=Aedes aegypti OX=7159 GN=AAEL005527 PE=1 SV=1[more]
Q9VIF13.1e-0921.82Exonuclease mut-7 homolog OS=Drosophila melanogaster OX=7227 GN=Nbr PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_004135767.10.098.15uncharacterized protein LOC101205010 isoform X1 [Cucumis sativus] >XP_031736262.... [more]
XP_031736264.10.099.25uncharacterized protein LOC101205010 isoform X2 [Cucumis sativus][more]
XP_008450552.10.093.70PREDICTED: uncharacterized protein LOC103492118 [Cucumis melo] >XP_008450553.1 P... [more]
KAA0050943.10.094.54exonuclease mut-7-like protein isoform X1 [Cucumis melo var. makuwa] >TYK10290.1... [more]
XP_038880060.10.088.70uncharacterized protein LOC120071757 isoform X1 [Benincasa hispida] >XP_03888006... [more]
Match NameE-valueIdentityDescription
A0A0A0LWB00.098.153'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G... [more]
A0A1S3BPE90.093.70uncharacterized protein LOC103492118 OS=Cucumis melo OX=3656 GN=LOC103492118 PE=... [more]
A0A5D3CG080.094.54Exonuclease mut-7-like protein isoform X1 OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A6J1HXY30.082.44uncharacterized protein LOC111467860 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1HC930.082.26uncharacterized protein LOC111462125 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT1G56310.12.5e-16855.43Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G24340.11.2e-1127.913'-5' exonuclease domain-containing protein [more]
AT4G13870.25.7e-1130.86Werner syndrome-like exonuclease [more]
AT4G13870.19.8e-1130.86Werner syndrome-like exonuclease [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0025623'-5' exonuclease domainSMARTSM0047435exoneu6coord: 365..542
e-value: 2.0E-7
score: 40.7
IPR0025623'-5' exonuclease domainPFAMPF01612DNA_pol_A_exo1coord: 367..539
e-value: 1.4E-22
score: 80.2
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 359..548
e-value: 3.9E-43
score: 148.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 549..586
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 541..586
NoneNo IPR availablePANTHERPTHR13620:SF91SUBFAMILY NOT NAMEDcoord: 56..346
NoneNo IPR availablePANTHERPTHR136203-5 EXONUCLEASEcoord: 56..346
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 324..540

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy1G025230.2CsGy1G025230.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010587 miRNA catabolic process
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0030422 production of siRNA involved in RNA interference
biological_process GO:0006139 nucleobase-containing compound metabolic process
cellular_component GO:0010494 cytoplasmic stress granule
molecular_function GO:0008408 3'-5' exonuclease activity
molecular_function GO:0003676 nucleic acid binding