CsGy1G024820 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy1G024820
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptiongamma-soluble NSF attachment protein
LocationGy14Chr1: 23762906 .. 23770588 (+)
RNA-Seq ExpressionCsGy1G024820
SyntenyCsGy1G024820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCAAAATAACCTTAAAGTTGATAGAGATATTGGAAATTTATTGGTTTATTTGTTGTTAATTTGGAAGAGATAGATAGAGGGAATTCTTACCTGTTGGATCGATTCGTTGCAATGGTGTAATCGTAAAGGGGAGTTGGAAGTACAGCTCGATCAAGCGTCGAGCACAATCACAACAATCAAGGGAGAAGAGAAGCAATTGCAGAACAGAAACAAGAACCATGTCTAGTTCCGATCCCGACAAGCTAATGATCAAAGCCGATAAATTGTTAGTTTTCTTCATTTCTTCATTTTCTCATCTGACTTCTTTCAACTTCGTTTTGTTTCTTATTAACTTCATGAACGGTGCCGAGCTTCTGAGGTTCTTATGGGATTTGGTTTTTTTTTTTTTTTTCTTTTAGTTTTGATTATGGATTTTGTAAGGGCTAGTTTGAAGTACCTTTCAGAAATGGGAAGGAAAATGAAAAATGTAATCAATGAGCCTAGGACAATGTGGGTTTTAAGCCATGCTTAATGTTGATGTGTAATTCTAGTGTTAGTTTCGAGGTGGATCGGATGGGTAGTTCATTTTTCAGGATCCATCTTTGAACTTATCCATTAAGTTTTCTTTGATTTATATTCATTTTCTTTATTTCCTGTAAAATCTATAGAACAAAACTCAGTATGACAAGATGGAGTGCTGATTGGAAAAGTGCTACTGTCCTCTATGAGCAGGCCGGTATGTTTATTTTCTATTCATGCAGTTACTTAAGCTCTCTAATGTGTTGCAAGATCATGAAACTTGCAGATTTTTGTATTACTTGTTATAATCTGTTCTCCCCAGATGATTTTAAATAAGAATCTGTCTTCTTTGCCTTAGCAAATGCATACCGGGTCCGAAATAACCATGAGAAAGCAAAGATTGCTTTTGAAAAAGCTTCAAAAGGGCAAGAGATGCTCTCCTCGTATCCTTGTTTCTAGAATTGGTTAATGGCTCTTTGGCCTGCATTCATTTATTTCTTGACCTTTGTTTTCTCCCTTTAAGTCCTTACTACTAATCAGACCCTGGGATGCCGCTAAGCATATGGAATCTGCTGGTGCTCTCGCAAAGGAACTTGGTAACTTGACTGAAGTTGCTGACTTTTACAGAAGAGCATCAGAGTTGTATGTTTTGTGTGGAAGATCTCAACCTGCATCTGATGCTTTATCAAAGGGTGCTCAGTAAGTTTATGTTTTATGCAACTTCACATAATTTTTGTCATTCAACCAAATTGCTTGAAACTAGATTTCTTCTTTTGTATCCTTTTATTCTTAACTAAATTGTTTCTAAACCACATAAAGGCTCTAAGTTAGCTTCGTGCAGTTGACAACATTATCAAAAATAAATTATATATAATTTTATACCTATAGTTTTTTTCAGTAGTTCTCTAGTAGAATCTTGGTTGTTTATGTTTCTTTGCCATTGCTGTTTCTCTAGCAGTAGGTTTCTGTCACCCAATTGAACAGCAGTTTCTTTCCTGCAAATTTTTTTAGAAAAATATCTTTGCGTTTAACGTACTTGACCTCTATGTTGACTCAATTAGTTTATATTCACCACAACTCCTTATCGTTTATGCCTACTGTTCTTCTTATACACAATTGCATTTGCCTTTTATTTGTTCATTCATGCTTGAGCAGCCACAGGTTAAAAACAAGAATAATAAAAAAGCTCCTCATTGAACTATTGCATCCTCCCTTCACTGGAATGGATGAACCATAACTGTAACTTAAGGGAATATGTCATTGCTTTTATATTTTACAAACATACAAGTTGATATAAGTACATCTTGCCATAAATTCCCTTGAGCATTTTAATTATATATCTCCTTACAGTGCTTTGGAAGATAGCAAGCCAGATGAAGCTATCACATTATACACTGAAGCCTGTACACTTCTGGAAGACGACGGCAGGGAACAAATGGCCTTTGATTTATATCGAGATGCCACAAGTGTTTTTGTAAAATTAGAGAAGTAAGTTTCAAGTAATTTTGAAACTGCCATAATCAAAATCTTAAAGTATTAACTTATTAGTTAGTTCACTATCTTATTATTTGCTATTTTATAATGAAATTTTTATTACCCATGTGCTGTCGAAACTACAATAAACGTTGTAGTAAAGGTGACAGCTAAACTTGCCCCTTGGACCCTAGGCCTAACTCCTTTAAATTTGAAGTAAGTAAGTCCTCTGTCTAGAAGAGTTTGAGTTCTATTGTTTTGTCGAGCATATTAGGGACAAAATGACTGCTTTAATTTTAGGCAAATACTCTATTTTATGAGCAGTACCATCTTAAACCTTTTAAAGTTTAAATATGAAATTTGAATCTTTCGTATCACTGTATCCTCAATTGTTTTCAAGTTCAGCTAATCTGTTTCTCATTTGATGCAATTATCTGTTTCTTTTAGCCAATTAATTGGGTATAGGCTTCTCTTGTACTTTGTTGTCCTATAACATCTTTTCAGAAGATATTGCTCTTTTTAAACTTTAAAGATACTATTGAACTGTTAATCTATTCCTAATACTCGGTAAATCTTAGGTCATGTATGAGCTTCCTTGTGTTCGTACATAGAAGGTTTAATTGCTGATAGACAGTATTGTCTTTTTCCATGTAGATATTCTGATGCTGCGGAATTGCTGTTGCGATGGGGTCTGGCAGCTGATAAATGCAATGCTGTCCATAGCCAGGGGAAGGTGGGAGCTATCTACTTCTTGAATTTTCTTATATTAATGTGCTACAATTTTTTCCTCTTCATTGAAACCAGCATGTTAAATGAGTTGCCAGGAAGATAACTGTCAGCTTTCTATCACATTGATCGTTTTCAGCCTTCCTCGGTGGATTTATGGGCTAATTTACAGCAGTCAATGATGTTTGAAAAGACTTTCCAATTGACATAAATGGAAGTTTAATATATACATATATATACATTTTTTTTTTTTGTTTCTTTTTATTAATCAAGAAAATTTTCGTAGAAAGAGAGCGATCTCTAATCTCTATCAGGAAATGGAGCTTATTATGATTTGCTAATAATACATTTATTAATTCCAGGCATATCTTAGTGCCATCATTGTTTACCTTTACGCGCATGACTTCAAACAAGCTGAGAAGTGCTACAATGACTGTTCACAGTGAGTACAAATTCTGAACTATGCCATGGCTTTCTTTTCATCTGCTTCATGGACTTTTATGCTTTATATTACATGATTAAAGCCAAGGTGGGTCATTTTCAAGTTAGCCTTTGGTAGATACTAATTATATACCAGATTTGGATTCACTTGATCTAAGAAGCACAAACATGGACACAGATACGACAAGAAACGGCAATTGCCAATTTCTAAAATACTAAGACACAGACATCTATTGGGGATACACCATTTTTTAAAATTGTTTTTTTAGAATATGTGTGCATTTAAGCTAAAATACTAAATGGGCTCCACTAAAAAAGCATAAAATGTCAACTGTCAAGTTCATTAATCATTGTCATCCAAACATTTAACAAATAATAAAAAAATATATATATGAGTTTCAACTTTGAAGATAGGAAAAAAAAATGGATTGATGAGTAAAAGGCAGTGGGCAGTAGGTAAAAGTACATATTGCAAATACCAACAGGCAGGGCTGGCAGAAACTTTTGCTCCAGCGAGAACTGGGAAGGCATTGTTTTTGATGCAGTTACTCGGAAGCAAGACAAGGTGGGGGATGGTTTTTTACTCTAAAAAGGAAGATAAATGAACTTTAACTTTGGCCCAAATTTTAAACAATTTTCTTTATTTTTTTTTCAGTGTGTCCGTGAAGTGTCCACTTTGTGTCCGAAATTAAAAAAAAAAAAAAAAAAATGCAAATTGGGTATGTTAGACACGTGTCCACTGCATGTTCGACACTGATATCACCTCATATGAATTTTGTGCTTCTCAGCACTTGATAAGAGGAATTGTAAATGAGATCCACTTTTAATAGAATCCTCAGCATCTGTTTCTATAAGTTGATAATTATGTCCTTGAGTTCAAAATGAGATCTTTGAAATTAAGAAGTGTCTGCATTGATTATTATCTCGATTATATTGTTTTATAGTTGTACAAGATGGGACATTTTGCTCAACTCAATACCAATCAAGTCCAGCAAATGATAAATGTCTGTTGTGTAGACTTAGTATTGGACAAAGATAGTAGTCATGAGTGTTGGGCAAATTGGACAGGAACAAGGTGAAGAGAGTCTTGAGAGTCTTTGTAATAAAGACTAAATCAGATGCATGTATATGCCTTCCTCTTTCTTTATTTTGTATAAATTAGCGGCACACTGATGATATTTAGTCAGATAGATACGATGCACATATGTGCATTTTCGTATCTTAATCAATATTAAATACATAAAAGCAGCGGAGTAGCCCAATTGATCTTCCCCTAATCTGAAAAACAAGCTAGTTGAGGCTAACTCTCCGTGACCACCTGCAGAATGGTTTGCACTCTTCTCCCTTCGTACATGGGCCAAGTGTTATTTTTTGGTTGATTTTTTCTTTCGACTTAGATAAATTCTTAGGAGTTTTTAAGCTTCCGAGAGATCAAAATAGGCTTTCCGTATCCAAGATAAAACTGGTGATGGATAATCTTTCATTCTTTAGTGTATTTAATTTTGAAAAAAGGGAATTGTTCCATTTTAATATTTTATTGCACATGACATCCTGAAAAGGTACTTACAGGTTGAAATTCTTTTATTTTCTCTTTGCAGAGTTGATGCTTTTACGAGAACTGACCAATACCGTGCTGCTAGTAAGCTATTATCTGCTTATAGGGAAGGCGATGTTGAGGAAATCAAGCGTGTGGCTCAGTCAAGCACTATTTCTCATCTTGATCATGTGGTAAATGTTTTTCCATGCTGTTTTCCATTTTCAGTTGATGAAAATGGTTGAATATGAAAGCTTTTTTTATTAACTACAATCAGTGAACCAGAACATCCTTTAATTCTCATGGGAAAAATTCCCCATTTTGAAAAGGAAGAGATAGAAGGGCAAAGAAAATGTAACTTTTCTTTGAAACTTCAACGTGCCGAAGAAAACATGATAATAAAGAAATGAGAACATTGTGTTGAGGGTTTAGGGTTTATGATAATAAAGAAATGTGAACTTTGTGTTGAAACACGGTAAACAAGGACAGGGAGAAAACAACCATCCAAGCATTTTTTCCCTTATAGTTATGTTTTCACTTTGGTATTCGAAACAAGTGAAAGACTTCATTACCTTGACATATAATTAAATGCAACAATGAGAAATTTCCCAAAATTTTGGTTCAAAATTGATTTTGTATGACCAATGGGCTTCTACATCAAGTTATAGAAACTTAAAAGTTTCTCGGTTCCTCCAAAGCTATTTCCTCTGCTACTCTTTATTCATAGCAATTAAAATGTACCCAGAACATTTCAATTCATGACTTTCCAAATAATTTGCCCAACCGACTTCACTTCGAAATTATGTAAAGAAATGTTAGATGTAGCTGTTGTAGCTTGTAGCGTTATCAGTTTTGCTTTGTATTGTTGTGTTACCTACTTTGAACAAAATAAAATTCCTGCAGATTATCAGGCTTGCTAGAAAACTGCCTACAGGTGATGTGAGTGCCCTGAAGACTGATACGACTGAAGACCAAGAAGAAGCACTGGATGAAAATGATCTCACATGATCTTTGGAATCATAAACTGCCACATATTTTCAGAACTTTGTTTTAAAAGATAATGGTTTCAACTTGCAATTATAGAGTGTAATTACTTTCTAAGAAATTTGTGTGAAAAATTATACCATGCGTGGGTTTTACTTGAAAAGCAAATGAAAAGCCATTAAACAGCTGAAGTTCTGTTTGCCAATTGTTCACCTTAGCCCTTGGGACTTGTGTACATAATATTAAAACATGGAAAAGAGAATGTCTAAAC

mRNA sequence

GCAAAATAACCTTAAAGTTGATAGAGATATTGGAAATTTATTGGTTTATTTGTTGTTAATTTGGAAGAGATAGATAGAGGGAATTCTTACCTGTTGGATCGATTCGTTGCAATGGTGTAATCGTAAAGGGGAGTTGGAAGTACAGCTCGATCAAGCGTCGAGCACAATCACAACAATCAAGGGAGAAGAGAAGCAATTGCAGAACAGAAACAAGAACCATGTCTAGTTCCGATCCCGACAAGCTAATGATCAAAGCCGATAAATTAACAAAACTCAGTATGACAAGATGGAGTGCTGATTGGAAAAGTGCTACTGTCCTCTATGAGCAGGCCGCAAATGCATACCGGGTCCGAAATAACCATGAGAAAGCAAAGATTGCTTTTGAAAAAGCTTCAAAAGGGCAAGAGATGCTCTCCTCACCCTGGGATGCCGCTAAGCATATGGAATCTGCTGGTGCTCTCGCAAAGGAACTTGGTAACTTGACTGAAGTTGCTGACTTTTACAGAAGAGCATCAGAGTTGTATGTTTTGTGTGGAAGATCTCAACCTGCATCTGATGCTTTATCAAAGGGTGCTCATGCTTTGGAAGATAGCAAGCCAGATGAAGCTATCACATTATACACTGAAGCCTGTACACTTCTGGAAGACGACGGCAGGGAACAAATGGCCTTTGATTTATATCGAGATGCCACAAGTGTTTTTGTAAAATTAGAGAAATATTCTGATGCTGCGGAATTGCTGTTGCGATGGGGTCTGGCAGCTGATAAATGCAATGCTGTCCATAGCCAGGGGAAGGCATATCTTAGTGCCATCATTGTTTACCTTTACGCGCATGACTTCAAACAAGCTGAGAAGTGCTACAATGACTGTTCACAAGTTGATGCTTTTACGAGAACTGACCAATACCGTGCTGCTAGTAAGCTATTATCTGCTTATAGGGAAGGCGATGTTGAGGAAATCAAGCGTGTGGCTCAGTCAAGCACTATTTCTCATCTTGATCATGTGATTATCAGGCTTGCTAGAAAACTGCCTACAGGTGATGTGAGTGCCCTGAAGACTGATACGACTGAAGACCAAGAAGAAGCACTGGATGAAAATGATCTCACATGATCTTTGGAATCATAAACTGCCACATATTTTCAGAACTTTGTTTTAAAAGATAATGGTTTCAACTTGCAATTATAGAGTGTAATTACTTTCTAAGAAATTTGTGTGAAAAATTATACCATGCGTGGGTTTTACTTGAAAAGCAAATGAAAAGCCATTAAACAGCTGAAGTTCTGTTTGCCAATTGTTCACCTTAGCCCTTGGGACTTGTGTACATAATATTAAAACATGGAAAAGAGAATGTCTAAAC

Coding sequence (CDS)

ATGTCTAGTTCCGATCCCGACAAGCTAATGATCAAAGCCGATAAATTAACAAAACTCAGTATGACAAGATGGAGTGCTGATTGGAAAAGTGCTACTGTCCTCTATGAGCAGGCCGCAAATGCATACCGGGTCCGAAATAACCATGAGAAAGCAAAGATTGCTTTTGAAAAAGCTTCAAAAGGGCAAGAGATGCTCTCCTCACCCTGGGATGCCGCTAAGCATATGGAATCTGCTGGTGCTCTCGCAAAGGAACTTGGTAACTTGACTGAAGTTGCTGACTTTTACAGAAGAGCATCAGAGTTGTATGTTTTGTGTGGAAGATCTCAACCTGCATCTGATGCTTTATCAAAGGGTGCTCATGCTTTGGAAGATAGCAAGCCAGATGAAGCTATCACATTATACACTGAAGCCTGTACACTTCTGGAAGACGACGGCAGGGAACAAATGGCCTTTGATTTATATCGAGATGCCACAAGTGTTTTTGTAAAATTAGAGAAATATTCTGATGCTGCGGAATTGCTGTTGCGATGGGGTCTGGCAGCTGATAAATGCAATGCTGTCCATAGCCAGGGGAAGGCATATCTTAGTGCCATCATTGTTTACCTTTACGCGCATGACTTCAAACAAGCTGAGAAGTGCTACAATGACTGTTCACAAGTTGATGCTTTTACGAGAACTGACCAATACCGTGCTGCTAGTAAGCTATTATCTGCTTATAGGGAAGGCGATGTTGAGGAAATCAAGCGTGTGGCTCAGTCAAGCACTATTTCTCATCTTGATCATGTGATTATCAGGCTTGCTAGAAAACTGCCTACAGGTGATGTGAGTGCCCTGAAGACTGATACGACTGAAGACCAAGAAGAAGCACTGGATGAAAATGATCTCACATGA

Protein sequence

MSSSDPDKLMIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASKGQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTEACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYREGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKTDTTEDQEEALDENDLT*
Homology
BLAST of CsGy1G024820 vs. ExPASy Swiss-Prot
Match: Q9SPE5 (Gamma-soluble NSF attachment protein OS=Arabidopsis thaliana OX=3702 GN=GSNAP PE=2 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 1.2e-115
Identity = 206/294 (70.07%), Postives = 253/294 (86.05%), Query Frame = 0

Query: 3   SSDPDKLMIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASKGQ 62
           SSDPDK+M KADK+TKL++TRWSADW+ AT LYEQAAN +R  N +EKAK+A EKASKGQ
Sbjct: 2   SSDPDKMMSKADKMTKLTLTRWSADWRGATELYEQAANGFRASNKYEKAKVALEKASKGQ 61

Query: 63  EMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHAL 122
           EM +SPWDAAKHMESA ALA++L    EVADFYR+ASELYV CGR+QPASDAL K A AL
Sbjct: 62  EMQASPWDAAKHMESAAALAQKLSIWNEVADFYRKASELYVECGRAQPASDALGKAARAL 121

Query: 123 EDSKPDEAITLYTEACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAAD 182
           ED KPD+AI LYT+AC +LE+DGR+QMAFDLYR   +V++KLEK++DAA   LR G+AAD
Sbjct: 122 EDVKPDDAIQLYTDACEILEEDGRDQMAFDLYRACANVYIKLEKFTDAATFFLRLGVAAD 181

Query: 183 KCNAVHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYREG 242
           KC+A +SQ KAYLSAII+YLYAHD +QAEKCYNDCSQ+DAF ++DQ R+AS+LL+AY EG
Sbjct: 182 KCDATNSQCKAYLSAIILYLYAHDLQQAEKCYNDCSQIDAFLKSDQSRSASRLLTAYNEG 241

Query: 243 DVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKTDTTEDQEEALDENDLT 297
           D+EEIK+VA +ST+S+LDH+II+LARKLPTGDV+A++ +T +D    LDE+DLT
Sbjct: 242 DIEEIKKVASASTVSNLDHMIIKLARKLPTGDVTAIQMNTNDD----LDEDDLT 291

BLAST of CsGy1G024820 vs. ExPASy Swiss-Prot
Match: Q9U9R7 (Gamma-soluble NSF attachment protein OS=Dictyostelium discoideum OX=44689 GN=snpC PE=1 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.6e-33
Identity = 84/264 (31.82%), Postives = 142/264 (53.79%), Query Frame = 0

Query: 7   DKLMIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASKGQEMLS 66
           +  M + DKL+K ++ RW ADW SA + YE+AANA+R    ++ AK  F + S  Q  + 
Sbjct: 14  ETFMKEGDKLSKTNIFRWKADWDSAALAYEKAANAFRSAKIYDSAKYCFLRLSLCQTHMD 73

Query: 67  SPWDAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSK 126
             + AAK ME+A A+AKEL    E A+    + +LY   G S  A+D ++K A  LED  
Sbjct: 74  VYYLAAKSMENASAMAKELKETQECANLLLESCKLYRTNGNSFQAADTMTKAAKLLEDID 133

Query: 127 PDEAITLYTEACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNA 186
            ++ I L T+AC L E D ++  + D ++   S+ +K +KY++A +L++       K   
Sbjct: 134 LNQTIKLLTDACELFELDDKDHFSGDTFKQTISMLLKHKKYTEAVDLMILQNRVFVKLEQ 193

Query: 187 VHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYREGDVEE 246
            H   K+ LS I + L   D   ++K Y       +F  + +   A +L++A+   DV+ 
Sbjct: 194 NHDLHKSCLSVITISLATDDIVASKKYYEQFLDYPSFIHSQEGTTAQELITAFDNHDVDG 253

Query: 247 IKRVAQSSTISHLDHVIIRLARKL 271
           +K++      + LD+ + ++A+ L
Sbjct: 254 VKKIVSRHIFNFLDNQVAKIAKNL 277

BLAST of CsGy1G024820 vs. ExPASy Swiss-Prot
Match: P81127 (Gamma-soluble NSF attachment protein OS=Bos taurus OX=9913 GN=NAPG PE=1 SV=3)

HSP 1 Score: 105.5 bits (262), Expect = 1.0e-21
Identity = 69/268 (25.75%), Postives = 130/268 (48.51%), Query Frame = 0

Query: 10  MIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASKGQEMLSSPW 69
           + KA+K  K    +W  D+ SA   Y +AA A++     E+AK A  K +   E   + +
Sbjct: 13  LAKAEKYLKTGFLKWKPDYDSAASEYGKAAVAFKNAKQFEQAKDACLKEAVAHENNRALF 72

Query: 70  DAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDE 129
            AAK  E AG + KE+  L E      +AS +Y+  G    A+ AL +    +E+  P++
Sbjct: 73  HAAKAYEQAGMMLKEMQKLPEAVQLIEKASMMYLENGTPDTAAMALERAGKLIENVDPEK 132

Query: 130 AITLYTEACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHS 189
           A+ LY +   + E++ R + A +L   A+ + V+  ++ +AA  + +      +     +
Sbjct: 133 AVQLYQQTANVFENEERLRQAVELLGKASRLLVRGRRFDEAAISIQKEKNIYKEIENYPT 192

Query: 190 QGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYREGDVEEIKR 249
             K  ++ ++V+L+ +D+  AE+C  +   +  F  ++   A  +LL  Y + D +++  
Sbjct: 193 CYKKTIAQVLVHLHRNDYVAAERCVRESYSIPGFNGSEDCAALEQLLEGYDQQDQDQVAE 252

Query: 250 VAQSSTISHLDHVIIRLARKL--PTGDV 276
           V  S    ++D+   +L   L  P G V
Sbjct: 253 VCNSPLFKYMDNDYAKLGLSLVVPGGGV 280

BLAST of CsGy1G024820 vs. ExPASy Swiss-Prot
Match: Q9CWZ7 (Gamma-soluble NSF attachment protein OS=Mus musculus OX=10090 GN=Napg PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 2.3e-21
Identity = 65/261 (24.90%), Postives = 127/261 (48.66%), Query Frame = 0

Query: 10  MIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASKGQEMLSSPW 69
           + KA+K  K    +W  D+ SA   Y +AA A++     E+AK A  + +   E   + +
Sbjct: 13  LAKAEKYLKTGFLKWKPDYDSAASEYGKAAVAFKNAKQFEQAKDACLREAVAHENNRALF 72

Query: 70  DAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDE 129
            AAK  E AG + KE+  L E      +AS +Y+  G    A+ AL +    +E+  P++
Sbjct: 73  HAAKAYEQAGMMLKEMQKLPEAVQLIEKASMMYLENGTPDTAAMALERAGKLIENVDPEK 132

Query: 130 AITLYTEACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHS 189
           A+ LY +   + E++ R + A +L   A+ + V+  ++ +AA  + +      +     +
Sbjct: 133 AVQLYQQTANVFENEERLRQAVELLGKASRLLVRGRRFDEAALSIQKEKNIYKEIENYPT 192

Query: 190 QGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYREGDVEEIKR 249
             K  ++ ++V+L+ +D+  AE+C  +   +  F  ++   A  +LL  Y + D +++  
Sbjct: 193 CYKKTIAQVLVHLHRNDYVAAERCVRESYSIPGFNGSEDCAALEQLLEGYDQQDQDQVSE 252

Query: 250 VAQSSTISHLDHVIIRLARKL 271
           V  S    ++D+   +L   L
Sbjct: 253 VCNSPLFKYMDNDYAKLGLSL 273

BLAST of CsGy1G024820 vs. ExPASy Swiss-Prot
Match: Q99747 (Gamma-soluble NSF attachment protein OS=Homo sapiens OX=9606 GN=NAPG PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 6.7e-21
Identity = 68/283 (24.03%), Postives = 135/283 (47.70%), Query Frame = 0

Query: 10  MIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASKGQEMLSSPW 69
           + KA+K  K    +W  D+ SA   Y +AA A++     E+AK A  + +   E   + +
Sbjct: 13  LAKAEKYLKTGFLKWKPDYDSAASEYGKAAVAFKNAKQFEQAKDACLREAVAHENNRALF 72

Query: 70  DAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDE 129
            AAK  E AG + KE+  L E      +AS +Y+  G    A+ AL +    +E+  P++
Sbjct: 73  HAAKAYEQAGMMLKEMQKLPEAVQLIEKASMMYLENGTPDTAAMALERAGKLIENVDPEK 132

Query: 130 AITLYTEACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHS 189
           A+ LY +   + E++ R + A +L   A+ + V+  ++ +AA  + +      +     +
Sbjct: 133 AVQLYQQTANVFENEERLRQAVELLGKASRLLVRGRRFDEAALSIQKEKNIYKEIENYPT 192

Query: 190 QGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYREGDVEEIKR 249
             K  ++ ++V+L+ +D+  AE+C  +   +  F  ++   A  +LL  Y + D +++  
Sbjct: 193 CYKKTIAQVLVHLHRNDYVAAERCVRESYSIPGFNGSEDCAALEQLLEGYDQQDQDQVSD 252

Query: 250 VAQSSTISHLDHVIIRLARKL--PTGDVSALKTDTTEDQEEAL 291
           V  S    ++D+   +L   L  P G +      T + + + +
Sbjct: 253 VCNSPLFKYMDNDYAKLGLSLVVPGGGIKKKSPATPQAKPDGV 295

BLAST of CsGy1G024820 vs. NCBI nr
Match: XP_004135563.1 (gamma-soluble NSF attachment protein [Cucumis sativus] >KGN65970.1 hypothetical protein Csa_023322 [Cucumis sativus])

HSP 1 Score: 581 bits (1498), Expect = 4.88e-209
Identity = 296/296 (100.00%), Postives = 296/296 (100.00%), Query Frame = 0

Query: 1   MSSSDPDKLMIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASK 60
           MSSSDPDKLMIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASK
Sbjct: 1   MSSSDPDKLMIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASK 60

Query: 61  GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAH 120
           GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAH
Sbjct: 61  GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAH 120

Query: 121 ALEDSKPDEAITLYTEACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLA 180
           ALEDSKPDEAITLYTEACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLA
Sbjct: 121 ALEDSKPDEAITLYTEACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLA 180

Query: 181 ADKCNAVHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYR 240
           ADKCNAVHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYR
Sbjct: 181 ADKCNAVHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYR 240

Query: 241 EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKTDTTEDQEEALDENDLT 296
           EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKTDTTEDQEEALDENDLT
Sbjct: 241 EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKTDTTEDQEEALDENDLT 296

BLAST of CsGy1G024820 vs. NCBI nr
Match: XP_008459116.1 (PREDICTED: gamma-soluble NSF attachment protein [Cucumis melo])

HSP 1 Score: 573 bits (1477), Expect = 7.75e-206
Identity = 290/296 (97.97%), Postives = 295/296 (99.66%), Query Frame = 0

Query: 1   MSSSDPDKLMIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASK 60
           MSSSDPDKLMIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASK
Sbjct: 1   MSSSDPDKLMIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASK 60

Query: 61  GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAH 120
           GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAH
Sbjct: 61  GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAH 120

Query: 121 ALEDSKPDEAITLYTEACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLA 180
           ALEDSKPDEAITLYTEACTLLE+DGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLA
Sbjct: 121 ALEDSKPDEAITLYTEACTLLEEDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLA 180

Query: 181 ADKCNAVHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYR 240
           ADKCNAVHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAF+RTDQYRAA+KLLSAYR
Sbjct: 181 ADKCNAVHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFSRTDQYRAATKLLSAYR 240

Query: 241 EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKTDTTEDQEEALDENDLT 296
           EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSA+KTDTT+DQEE LDENDLT
Sbjct: 241 EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSAMKTDTTDDQEEPLDENDLT 296

BLAST of CsGy1G024820 vs. NCBI nr
Match: XP_038893768.1 (gamma-soluble NSF attachment protein [Benincasa hispida])

HSP 1 Score: 557 bits (1436), Expect = 1.38e-199
Identity = 282/296 (95.27%), Postives = 287/296 (96.96%), Query Frame = 0

Query: 1   MSSSDPDKLMIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASK 60
           MSSSDPDKLMIKADKLTKLSMTRWSADWK ATVLYEQAANAYRVRNNHEKAKIAFEKASK
Sbjct: 1   MSSSDPDKLMIKADKLTKLSMTRWSADWKGATVLYEQAANAYRVRNNHEKAKIAFEKASK 60

Query: 61  GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAH 120
           GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELY+LCGRSQPASDALSKGAH
Sbjct: 61  GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYILCGRSQPASDALSKGAH 120

Query: 121 ALEDSKPDEAITLYTEACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLA 180
           ALED KPDEAITLYT+ACTLLE+DGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLA
Sbjct: 121 ALEDGKPDEAITLYTDACTLLEEDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLA 180

Query: 181 ADKCNAVHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYR 240
           ADKCNAVHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVD F RTDQYRAASKL+SAY 
Sbjct: 181 ADKCNAVHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDTFLRTDQYRAASKLISAYI 240

Query: 241 EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKTDTTEDQEEALDENDLT 296
           EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKTD TE+  E LDENDLT
Sbjct: 241 EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKTDATEEHGEPLDENDLT 296

BLAST of CsGy1G024820 vs. NCBI nr
Match: XP_022927536.1 (gamma-soluble NSF attachment protein [Cucurbita moschata] >XP_023000740.1 gamma-soluble NSF attachment protein [Cucurbita maxima] >KAG6583818.1 Gamma-soluble NSF attachment protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019444.1 Gamma-soluble NSF attachment protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 546 bits (1407), Expect = 3.62e-195
Identity = 275/296 (92.91%), Postives = 285/296 (96.28%), Query Frame = 0

Query: 1   MSSSDPDKLMIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASK 60
           MSSSD DKLMIKADKLTKLSMTRW+ADWK+ATVLYEQAANAYRVRNN E AKIAFEKASK
Sbjct: 1   MSSSDADKLMIKADKLTKLSMTRWNADWKTATVLYEQAANAYRVRNNRENAKIAFEKASK 60

Query: 61  GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAH 120
           GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELY+LCGRSQPASDALSKGAH
Sbjct: 61  GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYILCGRSQPASDALSKGAH 120

Query: 121 ALEDSKPDEAITLYTEACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLA 180
           ALED KPDEAITLYT+ACTLLE+DGREQMAFDLYRDATSVFVKLEKYS+AAELLLRWGLA
Sbjct: 121 ALEDGKPDEAITLYTDACTLLEEDGREQMAFDLYRDATSVFVKLEKYSEAAELLLRWGLA 180

Query: 181 ADKCNAVHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYR 240
           ADKCNA HSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAF RTDQYRAA+KL+SAY 
Sbjct: 181 ADKCNATHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFLRTDQYRAANKLISAYM 240

Query: 241 EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKTDTTEDQEEALDENDLT 296
           EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALK D T++ EE LDENDLT
Sbjct: 241 EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKADATDEHEEPLDENDLT 296

BLAST of CsGy1G024820 vs. NCBI nr
Match: XP_023519196.1 (gamma-soluble NSF attachment protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 545 bits (1404), Expect = 1.04e-194
Identity = 274/296 (92.57%), Postives = 285/296 (96.28%), Query Frame = 0

Query: 1   MSSSDPDKLMIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASK 60
           MSSSD DKLMIKADKLTKLSMTRW+ADWK+ATVLYEQAANAYRVRNN E AKIAFEKASK
Sbjct: 1   MSSSDADKLMIKADKLTKLSMTRWNADWKTATVLYEQAANAYRVRNNRENAKIAFEKASK 60

Query: 61  GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAH 120
           GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELY+LCGRSQPASDALSKGAH
Sbjct: 61  GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYILCGRSQPASDALSKGAH 120

Query: 121 ALEDSKPDEAITLYTEACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLA 180
           ALED KPDEAITLYT+ACTLLE+DGREQMAFDLYRDATSVFVKLEKYS+AAELLLRWGLA
Sbjct: 121 ALEDGKPDEAITLYTDACTLLEEDGREQMAFDLYRDATSVFVKLEKYSEAAELLLRWGLA 180

Query: 181 ADKCNAVHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYR 240
           ADKCNA HSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAF RTDQYRAA+KL++AY 
Sbjct: 181 ADKCNATHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFLRTDQYRAANKLIAAYM 240

Query: 241 EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKTDTTEDQEEALDENDLT 296
           EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALK D T++ EE LDENDLT
Sbjct: 241 EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKADATDEHEEPLDENDLT 296

BLAST of CsGy1G024820 vs. ExPASy TrEMBL
Match: A0A0A0LVP4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G554570 PE=3 SV=1)

HSP 1 Score: 581 bits (1498), Expect = 2.36e-209
Identity = 296/296 (100.00%), Postives = 296/296 (100.00%), Query Frame = 0

Query: 1   MSSSDPDKLMIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASK 60
           MSSSDPDKLMIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASK
Sbjct: 1   MSSSDPDKLMIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASK 60

Query: 61  GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAH 120
           GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAH
Sbjct: 61  GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAH 120

Query: 121 ALEDSKPDEAITLYTEACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLA 180
           ALEDSKPDEAITLYTEACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLA
Sbjct: 121 ALEDSKPDEAITLYTEACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLA 180

Query: 181 ADKCNAVHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYR 240
           ADKCNAVHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYR
Sbjct: 181 ADKCNAVHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYR 240

Query: 241 EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKTDTTEDQEEALDENDLT 296
           EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKTDTTEDQEEALDENDLT
Sbjct: 241 EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKTDTTEDQEEALDENDLT 296

BLAST of CsGy1G024820 vs. ExPASy TrEMBL
Match: A0A1S3CAN0 (gamma-soluble NSF attachment protein OS=Cucumis melo OX=3656 GN=LOC103498320 PE=3 SV=1)

HSP 1 Score: 573 bits (1477), Expect = 3.75e-206
Identity = 290/296 (97.97%), Postives = 295/296 (99.66%), Query Frame = 0

Query: 1   MSSSDPDKLMIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASK 60
           MSSSDPDKLMIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASK
Sbjct: 1   MSSSDPDKLMIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASK 60

Query: 61  GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAH 120
           GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAH
Sbjct: 61  GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAH 120

Query: 121 ALEDSKPDEAITLYTEACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLA 180
           ALEDSKPDEAITLYTEACTLLE+DGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLA
Sbjct: 121 ALEDSKPDEAITLYTEACTLLEEDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLA 180

Query: 181 ADKCNAVHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYR 240
           ADKCNAVHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAF+RTDQYRAA+KLLSAYR
Sbjct: 181 ADKCNAVHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFSRTDQYRAATKLLSAYR 240

Query: 241 EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKTDTTEDQEEALDENDLT 296
           EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSA+KTDTT+DQEE LDENDLT
Sbjct: 241 EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSAMKTDTTDDQEEPLDENDLT 296

BLAST of CsGy1G024820 vs. ExPASy TrEMBL
Match: A0A6J1KJ73 (gamma-soluble NSF attachment protein OS=Cucurbita maxima OX=3661 GN=LOC111495099 PE=3 SV=1)

HSP 1 Score: 546 bits (1407), Expect = 1.75e-195
Identity = 275/296 (92.91%), Postives = 285/296 (96.28%), Query Frame = 0

Query: 1   MSSSDPDKLMIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASK 60
           MSSSD DKLMIKADKLTKLSMTRW+ADWK+ATVLYEQAANAYRVRNN E AKIAFEKASK
Sbjct: 1   MSSSDADKLMIKADKLTKLSMTRWNADWKTATVLYEQAANAYRVRNNRENAKIAFEKASK 60

Query: 61  GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAH 120
           GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELY+LCGRSQPASDALSKGAH
Sbjct: 61  GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYILCGRSQPASDALSKGAH 120

Query: 121 ALEDSKPDEAITLYTEACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLA 180
           ALED KPDEAITLYT+ACTLLE+DGREQMAFDLYRDATSVFVKLEKYS+AAELLLRWGLA
Sbjct: 121 ALEDGKPDEAITLYTDACTLLEEDGREQMAFDLYRDATSVFVKLEKYSEAAELLLRWGLA 180

Query: 181 ADKCNAVHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYR 240
           ADKCNA HSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAF RTDQYRAA+KL+SAY 
Sbjct: 181 ADKCNATHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFLRTDQYRAANKLISAYM 240

Query: 241 EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKTDTTEDQEEALDENDLT 296
           EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALK D T++ EE LDENDLT
Sbjct: 241 EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKADATDEHEEPLDENDLT 296

BLAST of CsGy1G024820 vs. ExPASy TrEMBL
Match: A0A6J1EHZ0 (gamma-soluble NSF attachment protein OS=Cucurbita moschata OX=3662 GN=LOC111434335 PE=3 SV=1)

HSP 1 Score: 546 bits (1407), Expect = 1.75e-195
Identity = 275/296 (92.91%), Postives = 285/296 (96.28%), Query Frame = 0

Query: 1   MSSSDPDKLMIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASK 60
           MSSSD DKLMIKADKLTKLSMTRW+ADWK+ATVLYEQAANAYRVRNN E AKIAFEKASK
Sbjct: 1   MSSSDADKLMIKADKLTKLSMTRWNADWKTATVLYEQAANAYRVRNNRENAKIAFEKASK 60

Query: 61  GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAH 120
           GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELY+LCGRSQPASDALSKGAH
Sbjct: 61  GQEMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYILCGRSQPASDALSKGAH 120

Query: 121 ALEDSKPDEAITLYTEACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLA 180
           ALED KPDEAITLYT+ACTLLE+DGREQMAFDLYRDATSVFVKLEKYS+AAELLLRWGLA
Sbjct: 121 ALEDGKPDEAITLYTDACTLLEEDGREQMAFDLYRDATSVFVKLEKYSEAAELLLRWGLA 180

Query: 181 ADKCNAVHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYR 240
           ADKCNA HSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAF RTDQYRAA+KL+SAY 
Sbjct: 181 ADKCNATHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFLRTDQYRAANKLISAYM 240

Query: 241 EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKTDTTEDQEEALDENDLT 296
           EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALK D T++ EE LDENDLT
Sbjct: 241 EGDVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKADATDEHEEPLDENDLT 296

BLAST of CsGy1G024820 vs. ExPASy TrEMBL
Match: A0A5D3BU63 (Gamma-soluble NSF attachment protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1734G00170 PE=3 SV=1)

HSP 1 Score: 536 bits (1381), Expect = 7.70e-192
Identity = 270/276 (97.83%), Postives = 275/276 (99.64%), Query Frame = 0

Query: 21  MTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASKGQEMLSSPWDAAKHMESAGA 80
           MTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASKGQEMLSSPWDAAKHMESAGA
Sbjct: 1   MTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASKGQEMLSSPWDAAKHMESAGA 60

Query: 81  LAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTEACTL 140
           LAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTEACTL
Sbjct: 61  LAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHALEDSKPDEAITLYTEACTL 120

Query: 141 LEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLSAIIV 200
           LE+DGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLSAIIV
Sbjct: 121 LEEDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAADKCNAVHSQGKAYLSAIIV 180

Query: 201 YLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYREGDVEEIKRVAQSSTISHLD 260
           YLYAHDFKQAEKCYNDCSQVDAF+RTDQYRAA+KLLSAYREGDVEEIKRVAQSSTISHLD
Sbjct: 181 YLYAHDFKQAEKCYNDCSQVDAFSRTDQYRAATKLLSAYREGDVEEIKRVAQSSTISHLD 240

Query: 261 HVIIRLARKLPTGDVSALKTDTTEDQEEALDENDLT 296
           HVIIRLARKLPTGDVSA+KTDTT+DQEE LDENDLT
Sbjct: 241 HVIIRLARKLPTGDVSAMKTDTTDDQEEPLDENDLT 276

BLAST of CsGy1G024820 vs. TAIR 10
Match: AT4G20410.1 (gamma-soluble NSF attachment protein )

HSP 1 Score: 417.5 bits (1072), Expect = 8.7e-117
Identity = 206/294 (70.07%), Postives = 253/294 (86.05%), Query Frame = 0

Query: 3   SSDPDKLMIKADKLTKLSMTRWSADWKSATVLYEQAANAYRVRNNHEKAKIAFEKASKGQ 62
           SSDPDK+M KADK+TKL++TRWSADW+ AT LYEQAAN +R  N +EKAK+A EKASKGQ
Sbjct: 2   SSDPDKMMSKADKMTKLTLTRWSADWRGATELYEQAANGFRASNKYEKAKVALEKASKGQ 61

Query: 63  EMLSSPWDAAKHMESAGALAKELGNLTEVADFYRRASELYVLCGRSQPASDALSKGAHAL 122
           EM +SPWDAAKHMESA ALA++L    EVADFYR+ASELYV CGR+QPASDAL K A AL
Sbjct: 62  EMQASPWDAAKHMESAAALAQKLSIWNEVADFYRKASELYVECGRAQPASDALGKAARAL 121

Query: 123 EDSKPDEAITLYTEACTLLEDDGREQMAFDLYRDATSVFVKLEKYSDAAELLLRWGLAAD 182
           ED KPD+AI LYT+AC +LE+DGR+QMAFDLYR   +V++KLEK++DAA   LR G+AAD
Sbjct: 122 EDVKPDDAIQLYTDACEILEEDGRDQMAFDLYRACANVYIKLEKFTDAATFFLRLGVAAD 181

Query: 183 KCNAVHSQGKAYLSAIIVYLYAHDFKQAEKCYNDCSQVDAFTRTDQYRAASKLLSAYREG 242
           KC+A +SQ KAYLSAII+YLYAHD +QAEKCYNDCSQ+DAF ++DQ R+AS+LL+AY EG
Sbjct: 182 KCDATNSQCKAYLSAIILYLYAHDLQQAEKCYNDCSQIDAFLKSDQSRSASRLLTAYNEG 241

Query: 243 DVEEIKRVAQSSTISHLDHVIIRLARKLPTGDVSALKTDTTEDQEEALDENDLT 297
           D+EEIK+VA +ST+S+LDH+II+LARKLPTGDV+A++ +T +D    LDE+DLT
Sbjct: 242 DIEEIKKVASASTVSNLDHMIIKLARKLPTGDVTAIQMNTNDD----LDEDDLT 291

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SPE51.2e-11570.07Gamma-soluble NSF attachment protein OS=Arabidopsis thaliana OX=3702 GN=GSNAP PE... [more]
Q9U9R72.6e-3331.82Gamma-soluble NSF attachment protein OS=Dictyostelium discoideum OX=44689 GN=snp... [more]
P811271.0e-2125.75Gamma-soluble NSF attachment protein OS=Bos taurus OX=9913 GN=NAPG PE=1 SV=3[more]
Q9CWZ72.3e-2124.90Gamma-soluble NSF attachment protein OS=Mus musculus OX=10090 GN=Napg PE=1 SV=1[more]
Q997476.7e-2124.03Gamma-soluble NSF attachment protein OS=Homo sapiens OX=9606 GN=NAPG PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_004135563.14.88e-209100.00gamma-soluble NSF attachment protein [Cucumis sativus] >KGN65970.1 hypothetical ... [more]
XP_008459116.17.75e-20697.97PREDICTED: gamma-soluble NSF attachment protein [Cucumis melo][more]
XP_038893768.11.38e-19995.27gamma-soluble NSF attachment protein [Benincasa hispida][more]
XP_022927536.13.62e-19592.91gamma-soluble NSF attachment protein [Cucurbita moschata] >XP_023000740.1 gamma-... [more]
XP_023519196.11.04e-19492.57gamma-soluble NSF attachment protein [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0LVP42.36e-209100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G554570 PE=3 SV=1[more]
A0A1S3CAN03.75e-20697.97gamma-soluble NSF attachment protein OS=Cucumis melo OX=3656 GN=LOC103498320 PE=... [more]
A0A6J1KJ731.75e-19592.91gamma-soluble NSF attachment protein OS=Cucurbita maxima OX=3661 GN=LOC111495099... [more]
A0A6J1EHZ01.75e-19592.91gamma-soluble NSF attachment protein OS=Cucurbita moschata OX=3662 GN=LOC1114343... [more]
A0A5D3BU637.70e-19297.83Gamma-soluble NSF attachment protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
AT4G20410.18.7e-11770.07gamma-soluble NSF attachment protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF14938SNAPcoord: 5..265
e-value: 1.1E-27
score: 97.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 277..296
NoneNo IPR availablePANTHERPTHR13768:SF2GAMMA-SOLUBLE NSF ATTACHMENT PROTEINcoord: 2..286
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 2..272
e-value: 7.6E-78
score: 263.5
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 6..250
IPR000744NSF attachment proteinPANTHERPTHR13768SOLUBLE NSF ATTACHMENT PROTEIN SNAPcoord: 2..286

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy1G024820.2CsGy1G024820.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006886 intracellular protein transport
cellular_component GO:0031201 SNARE complex
molecular_function GO:0005483 soluble NSF attachment protein activity
molecular_function GO:0019905 syntaxin binding
molecular_function GO:0005515 protein binding