CsGy1G024210 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy1G024210
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionFAD-binding Berberine family protein
LocationGy14Chr1: 23044454 .. 23048466 (+)
RNA-Seq ExpressionCsGy1G024210
SyntenyCsGy1G024210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATCTTTCTAAAACCAAGAAAACAAACGCAAACAAACACTAGAATGGAAATTTTAAGGACTTCAATTTTGCTCTTATCATTTTCATTCCTTGTGTTTTTTTCTTCTGCGTCTTCTTCAGCTCCTGAAAATTTCTTTCAATGTCTTCTCCGACACTCTCCACCATCGTACCCCATCGCTCCGGCTATCTTTACTCCAAACAATGCTTCTTATTCCTCCGTTCTCGAGACTTACATTAGAAACTTGAGATTCAACACTTCAACAACTCCTAAACCATTCCTCATTCTCACTGCTCTCCATGAATCTCATGTTAAAGCTGCTATTCTTTGTGCTCGAATCCATAATCTTCAAATGAAGATTCGTAGCGGCGGCCATGATTACGAGGGCGTTTCTTACGTCTCCGACGTCGTCCCTTTCTTCATACTCGATATGTTCAACCTACGAGAGATCACGGTTGATGCCAAAACCAAGACCGCTTGGGTATTGTCAATCATTCATTTATCTTTGATATAGTTTATTGTCTCTCTCAACTTATTGTAATCCTTGTACATCCAGGCAGCTTTCATTTATTATTTTTAAATTGATTTTTGATAATTTATTTTCGTGTGAAAAGAAAAGGGTATAAAAAATGAATTAGATGTAGCCTTATACTAGTCATCCGTACGTAATATTATTATCAATGTTTATTTACTTAGGAAATAACATAATGCAGTGTTAAATGACCTATGCTACGGCTTAAGATTGAAATCTCTATAGACTGCATTATATTTAATCATTTATTCATATTGTTACCAGGTCCAAACTGGAGCAACTCTAGGAGAAGTATATTATAGAGTTGCTGAGAATAACAAGTCCTACGGCTTCCCAGCCGGCGTGTGCCCCACGGTAGGGGTCGGCGGTCATTTTGGTGGTGGTGGTTACGGTAACATGATGAGAAAATACGGCCTCTCCGTGGATCAAATAATTGATGCAAAGATTATCGACGTCAATGGAAAACTCCTTGACCGAAAGGCAATGGGGGAGGATCTATTTTGGGCCATCATAGGCGGTGGAGGATCTAGCTTCGGTGTCGTTGTAGCATACAAAATCCGAATGGTTCCTGTGCCGGAGACGGTAACAGTGTTCCGAGTACAAAGAACATTGGAACAAAATGACTTAACGGAGATTGTAGATGAGTGGCAACAAGTGGCACATGTGATTGACAATGATTTGTTCATAAGAGTAACCTTTGATGTTGTCAATGGAACAAACAAGGGGAAAAAAACATTGAGAGCTACGTTTATAGCCTTGTTTCTTGGAGATTCAGAAAGGCTTTTGTTTGTCATCAACAACAGTTTTCCTAAACTTGGTTTGAAGAAATCTGATTGCATTGAAATGAGTTGGCTTCAATCTGTGCTCTTTTGGACTAATTTCCCTCTAGGTAATCTTTCTTTTTACTTTGGATATATAACAGTTTGTTCTTCGTCAAATTAATGACTTTTATTTTTTTGTTGTTTTTAAAATGCTTTTGAAAACACCTCCATAAAAGAGGATATCACAGACATTATAGGAACTAAGAAAACTTGTGCCACAAGTTTTTTTCTTTATTTTTCTTAATAGAAATATTGAATTTTTCTATTAAAAAATTAGAGAAAACTTGTGCCATAATCTTTAGTAATTGTTGTTTTCTTTTTTCTTGTTTATTAGTTTTTTTATTTTTTTATGAAAAAAATTAAGACTTCTAGTGGTTCTTTTTTTAATGGAAAAATGATTCACATATATATTATATATAGAAAAGTTTTCAAATATGAAGTATAATGCCCTTTTAATAAAATACCCAAACCAGATTTTTCAAAGTTAAAAGAAATAGTAAAAACCATTTTTCTTACTTTCGTTAAAAAACCAACTCTGGTAATTAGAAAACATTTAATAGGAAGGAAATAAGTTAATTTTCAAAAATCAAAAACAAAAAATAGAACATTACTAAAACTGAGTAATCATTTATTGTTAGTTTTTAGTTTTTAGAAAAATAAACTTACAATCAATACTCTTATTTTTACGTTTTTAATATATATATATTTTTTCTACCGATCTTTTAAAAATGCAACGTCAAATTCTTTTAAATAAAAACTAGAGAAATTATTTTAAATAATAAAAAATTTTAAAATAATTCTAAAAATATTTACAACTATAGCAAAACATTACAATCTAGATGTGATATATAGATCAAGATAGACTATTTAGACACATGATATAGGTAGACAAAATAATAGTTTATCTTGGTCTATCATGTCCATCATAGATAGATTATGATATATTTCTATATTTTATTAAATTTTTTTATTACTTTTTCTATATTTTGAAAATAACTCTAAAATTGTTTTGAAATTGCTAAATCGAGACGTTTTGTTTAAGAAAACAAAGTTAACGAGAAAAGGAAAATAAGTATAAACTTTCAAAATCAAAATACTTATTACTAAATTGTTTGGATTGAGAAATATAGTAAAGAAGAGAGATCACATGATAGATTTATGATTTAAAACCGAACTCGTTTTAAACAAAAAGAAAACTTGGGTGCTCCTTTTGGCGGGACGTATGTTGATTCAATCTACATGGGATCGACAATTATAACAAATTTTGTAAGAAATTTTTTTGGCACACCCTACATTTAAAAATGAATTTGAGAAAGAAAAAATGTGATTGTGGCTAAAACTCTTGGAATCAATTTTTCTTTTTGAAGAAAAACAACATATTTTTATTTTGTTGAATTGAAAATCCCCCACGAGTTTTTATAATATAATAATGACCAAACTATCCTTTCCTCATCTCTCTCAAATCTGTCCCACCATGACTAGACAACACATCATAATATTTTAAATACAAATTTGACTTTATTAGTAGATGTATTTAGAGCCAACCAAAATACAAAACTATGAAAAAAAGAAAAAAAGCTCTTCTCTAACAACGCAAACAAACCTATAATTGTTTTTAAGAAAATTACTTTTAAAAAAACTTTCCTAAATATAACAAAATATAACGTATCTATGTCTACCGTTAGACACATATCAATACAAATGGTAGTACATTGCAGTCTATACATTACAGTTTGATAGACGTCAAATTTTGTTATATTTATAAATATGTTATAGAATCGATAGAAAATAATATAAAAAATATTTGGAAAGATGAGACCCTATGATCTAAAGTTATTCTTTTATGAAATATTTGTAACCACAGGCACATCGGTGGAAGCCCTACTGTCCAGAACCCCACAAGTACTGACCCATCTAAAAAGAAAGTCAGATTATGTAAAAACCCCAATTCCAAAGGAAGGACTCAACAAAATATGGAAGAAAATGATAGAACTTGAAAAACCCATGTTGACATTCAACCCTTATGGTGGAAGAATGGCTGAAATTCCATCAAATGCAACTCCATTTCCTCATAGAGCTGGAAACTTATGGAAGATTCAATATGCAACAAATTGGGTTGAAGAAGGAAACAAAGAAGCCAAACATTTTATTGATTTGACAAGGAAACTCTACAAGTTTATGACACCATTTGTGTCAAAAAATCCAAGAACTGCCTTTCTCAACTATAGAGATCTTGATATTGGTGTAAATCACAATGGGAAGAATAGTTACTATGAAGGAAGAGTTTATGGAATTAAATATTTTGAAGGTAACTTTGATAGGTTGGTGAAGATCAAGACCAAGGTTGATCCTCACAACTTTTTTAGGAATGAACAAAGCATTCCAAGGTTTCCTCATGTTTGGTGATACAAAATTATTGTGTGTTATTGTTTCTCTTTTGTTGTTTTCCTTGTTAATTGTCATTTGAAAGATCAACGTTTTCTTTGCTAAATAATATATTAAAGTGTGTAAGTTATATATACACGGGATATATATTATATAGTTGTATGTGTGTGATAGATGTGATGATTAGGCTATAAAGTCATTCTTCATAATGCATTTTTATGTATGTGAAAGTGAAAGGGCCCCTTTAAGTTTATCATATATTCAACTAAAGATGTGTC

mRNA sequence

CATCTTTCTAAAACCAAGAAAACAAACGCAAACAAACACTAGAATGGAAATTTTAAGGACTTCAATTTTGCTCTTATCATTTTCATTCCTTGTGTTTTTTTCTTCTGCGTCTTCTTCAGCTCCTGAAAATTTCTTTCAATGTCTTCTCCGACACTCTCCACCATCGTACCCCATCGCTCCGGCTATCTTTACTCCAAACAATGCTTCTTATTCCTCCGTTCTCGAGACTTACATTAGAAACTTGAGATTCAACACTTCAACAACTCCTAAACCATTCCTCATTCTCACTGCTCTCCATGAATCTCATGTTAAAGCTGCTATTCTTTGTGCTCGAATCCATAATCTTCAAATGAAGATTCGTAGCGGCGGCCATGATTACGAGGGCGTTTCTTACGTCTCCGACGTCGTCCCTTTCTTCATACTCGATATGTTCAACCTACGAGAGATCACGGTTGATGCCAAAACCAAGACCGCTTGGGTCCAAACTGGAGCAACTCTAGGAGAAGTATATTATAGAGTTGCTGAGAATAACAAGTCCTACGGCTTCCCAGCCGGCGTGTGCCCCACGGTAGGGGTCGGCGGTCATTTTGGTGGTGGTGGTTACGGTAACATGATGAGAAAATACGGCCTCTCCGTGGATCAAATAATTGATGCAAAGATTATCGACGTCAATGGAAAACTCCTTGACCGAAAGGCAATGGGGGAGGATCTATTTTGGGCCATCATAGGCGGTGGAGGATCTAGCTTCGGTGTCGTTGTAGCATACAAAATCCGAATGGTTCCTGTGCCGGAGACGGTAACAGTGTTCCGAGTACAAAGAACATTGGAACAAAATGACTTAACGGAGATTGTAGATGAGTGGCAACAAGTGGCACATGTGATTGACAATGATTTGTTCATAAGAGTAACCTTTGATGTTGTCAATGGAACAAACAAGGGGAAAAAAACATTGAGAGCTACGTTTATAGCCTTGTTTCTTGGAGATTCAGAAAGGCTTTTGTTTGTCATCAACAACAGTTTTCCTAAACTTGGTTTGAAGAAATCTGATTGCATTGAAATGAGTTGGCTTCAATCTGTGCTCTTTTGGACTAATTTCCCTCTAGGCACATCGGTGGAAGCCCTACTGTCCAGAACCCCACAAGTACTGACCCATCTAAAAAGAAAGTCAGATTATGTAAAAACCCCAATTCCAAAGGAAGGACTCAACAAAATATGGAAGAAAATGATAGAACTTGAAAAACCCATGTTGACATTCAACCCTTATGGTGGAAGAATGGCTGAAATTCCATCAAATGCAACTCCATTTCCTCATAGAGCTGGAAACTTATGGAAGATTCAATATGCAACAAATTGGGTTGAAGAAGGAAACAAAGAAGCCAAACATTTTATTGATTTGACAAGGAAACTCTACAAGTTTATGACACCATTTGTGTCAAAAAATCCAAGAACTGCCTTTCTCAACTATAGAGATCTTGATATTGGTGTAAATCACAATGGGAAGAATAGTTACTATGAAGGAAGAGTTTATGGAATTAAATATTTTGAAGGTAACTTTGATAGGTTGGTGAAGATCAAGACCAAGGTTGATCCTCACAACTTTTTTAGGAATGAACAAAGCATTCCAAGGTTTCCTCATGTTTGGTGATACAAAATTATTGTGTGTTATTGTTTCTCTTTTGTTGTTTTCCTTGTTAATTGTCATTTGAAAGATCAACGTTTTCTTTGCTAAATAATATATTAAAGTGTGTAAGTTATATATACACGGGATATATATTATATAGTTGTATGTGTGTGATAGATGTGATGATTAGGCTATAAAGTCATTCTTCATAATGCATTTTTATGTATGTGAAAGTGAAAGGGCCCCTTTAAGTTTATCATATATTCAACTAAAGATGTGTC

Coding sequence (CDS)

ATGGAAATTTTAAGGACTTCAATTTTGCTCTTATCATTTTCATTCCTTGTGTTTTTTTCTTCTGCGTCTTCTTCAGCTCCTGAAAATTTCTTTCAATGTCTTCTCCGACACTCTCCACCATCGTACCCCATCGCTCCGGCTATCTTTACTCCAAACAATGCTTCTTATTCCTCCGTTCTCGAGACTTACATTAGAAACTTGAGATTCAACACTTCAACAACTCCTAAACCATTCCTCATTCTCACTGCTCTCCATGAATCTCATGTTAAAGCTGCTATTCTTTGTGCTCGAATCCATAATCTTCAAATGAAGATTCGTAGCGGCGGCCATGATTACGAGGGCGTTTCTTACGTCTCCGACGTCGTCCCTTTCTTCATACTCGATATGTTCAACCTACGAGAGATCACGGTTGATGCCAAAACCAAGACCGCTTGGGTCCAAACTGGAGCAACTCTAGGAGAAGTATATTATAGAGTTGCTGAGAATAACAAGTCCTACGGCTTCCCAGCCGGCGTGTGCCCCACGGTAGGGGTCGGCGGTCATTTTGGTGGTGGTGGTTACGGTAACATGATGAGAAAATACGGCCTCTCCGTGGATCAAATAATTGATGCAAAGATTATCGACGTCAATGGAAAACTCCTTGACCGAAAGGCAATGGGGGAGGATCTATTTTGGGCCATCATAGGCGGTGGAGGATCTAGCTTCGGTGTCGTTGTAGCATACAAAATCCGAATGGTTCCTGTGCCGGAGACGGTAACAGTGTTCCGAGTACAAAGAACATTGGAACAAAATGACTTAACGGAGATTGTAGATGAGTGGCAACAAGTGGCACATGTGATTGACAATGATTTGTTCATAAGAGTAACCTTTGATGTTGTCAATGGAACAAACAAGGGGAAAAAAACATTGAGAGCTACGTTTATAGCCTTGTTTCTTGGAGATTCAGAAAGGCTTTTGTTTGTCATCAACAACAGTTTTCCTAAACTTGGTTTGAAGAAATCTGATTGCATTGAAATGAGTTGGCTTCAATCTGTGCTCTTTTGGACTAATTTCCCTCTAGGCACATCGGTGGAAGCCCTACTGTCCAGAACCCCACAAGTACTGACCCATCTAAAAAGAAAGTCAGATTATGTAAAAACCCCAATTCCAAAGGAAGGACTCAACAAAATATGGAAGAAAATGATAGAACTTGAAAAACCCATGTTGACATTCAACCCTTATGGTGGAAGAATGGCTGAAATTCCATCAAATGCAACTCCATTTCCTCATAGAGCTGGAAACTTATGGAAGATTCAATATGCAACAAATTGGGTTGAAGAAGGAAACAAAGAAGCCAAACATTTTATTGATTTGACAAGGAAACTCTACAAGTTTATGACACCATTTGTGTCAAAAAATCCAAGAACTGCCTTTCTCAACTATAGAGATCTTGATATTGGTGTAAATCACAATGGGAAGAATAGTTACTATGAAGGAAGAGTTTATGGAATTAAATATTTTGAAGGTAACTTTGATAGGTTGGTGAAGATCAAGACCAAGGTTGATCCTCACAACTTTTTTAGGAATGAACAAAGCATTCCAAGGTTTCCTCATGTTTGGTGA

Protein sequence

MEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVLETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGGHFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEALLSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHVW*
Homology
BLAST of CsGy1G024210 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 2.5e-196
Identity = 339/526 (64.45%), Postives = 411/526 (78.14%), Query Frame = 0

Query: 8   ILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVLETYIRNL 67
           IL+L F   ++ SS+SS+  E F QCL  +S P +PI+PAIF   N SYSSVL+  IRNL
Sbjct: 6   ILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIRNL 65

Query: 68  RFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVS-DVVPFFI 127
           RFNT++TPKPFLI+ A HESHV+AAI C + HNLQMKIRSGGHDY+G+SYV+    PFF+
Sbjct: 66  RFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFV 125

Query: 128 LDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGGHFGGGG 187
           LDMFNLR + VD  +KTAWVQTGA LGEVYY + E +K+  +PAG+CPTVGVGGH  GGG
Sbjct: 126 LDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGG 185

Query: 188 YGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRMV 247
           YGNMMRKYGL+VD  IDA+++DVNGK+LDRK MGEDL+WAI GGGG S+GVV+AYKI +V
Sbjct: 186 YGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLV 245

Query: 248 PVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKTLRAT 307
            VPE VTVFR+ RTLEQN  T+I+  WQQVA  + ++LFIR   DVVNGT   +KT+R T
Sbjct: 246 EVPENVTVFRISRTLEQN-ATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRTT 305

Query: 308 FIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEALLSRTPQ 367
           FIA+FLGD+  LL ++N  FP+LGL +SDC E SW+QSVLFWTN  +G+S E LL +  Q
Sbjct: 306 FIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSS-ETLLLQRNQ 365

Query: 368 VLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPHRAG 427
            + +LKRKSDYV+ PI + GL  IWKKMIELE P + FNPYGG M  I S  TPFP+RAG
Sbjct: 366 PVNYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAG 425

Query: 428 NLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVN-HNG 487
           NLWKIQY  NW +E   +   +++LTRKLY+FMTPFVSKNPR +F NYRD+D+G+N HNG
Sbjct: 426 NLWKIQYGANWRDETLTD--RYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNG 485

Query: 488 K-NSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP 531
           K +SY EG+ YG KYF GNF+RLVKIKT+VD  NFFRNEQSIP  P
Sbjct: 486 KISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVLP 527

BLAST of CsGy1G024210 vs. ExPASy Swiss-Prot
Match: Q9SVG3 (Berberine bridge enzyme-like 21 OS=Arabidopsis thaliana OX=3702 GN=At4g20840 PE=2 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 8.8e-165
Identity = 296/519 (57.03%), Postives = 374/519 (72.06%), Query Frame = 0

Query: 16  LVFFSSA----SSSAPENFFQCLL-RHSPPSYPIAPAIFTPNNASYSSVLETYIRNLRFN 75
           L FFSSA    S S  E+F QC   +   P   I   +F+  N S+SSVL  YIRN RFN
Sbjct: 20  LPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSSVLRAYIRNGRFN 79

Query: 76  TSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFFILDMF 135
           TS+TPKP +I+T   + HV AA+ C++  N  +KIRSGGHDYEG+SY+SD  PFFILDM 
Sbjct: 80  TSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYISD-KPFFILDMS 139

Query: 136 NLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGGHFGGGGYGNM 195
           NLR+++VD   ++AW+  GATLGEVYYR+ E +K +GFPAGVCPTVGVGGH  GGGYGNM
Sbjct: 140 NLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGVGGHISGGGYGNM 199

Query: 196 MRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRMVPVPE 255
           +RK+GLSVD +IDAKI+DVNG++LDRK+MGEDLFWAI GGGG+SFGVV+ YK+++VPVPE
Sbjct: 200 LRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVLGYKVKLVPVPE 259

Query: 256 TVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKTLRATFIAL 315
           TVTVFRV++ ++ +   ++V +WQ V    D +LF+R+    V  T K  KT+RAT +AL
Sbjct: 260 TVTVFRVEKYMD-SGAVDMVHKWQSVGPKTDRNLFLRMLIQPV--TRKKVKTVRATVVAL 319

Query: 316 FLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVE--ALLSRTPQVL 375
           FLG +E ++ ++   FP+L LKK +C EM+W QS L+W N    T ++    L R     
Sbjct: 320 FLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDPKVFLDRNLDRA 379

Query: 376 THLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPHRAGNL 435
              KRKSDYV + IP++G+  ++KKM EL K  L FNPYGG+MAE+  NATPFPHR+  L
Sbjct: 380 NFGKRKSDYVASEIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTVNATPFPHRS-KL 439

Query: 436 WKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVNHNGKNS 495
           +KIQY+  W E   +  K F++    LY FMT FVSKNPR A+LNYRD+DIGVN +G NS
Sbjct: 440 FKIQYSVTWQENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRDVDIGVNDHGTNS 499

Query: 496 YYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIP 528
           Y EG VYG KYF  NFDRLVK+KT  DP NFFRNEQSIP
Sbjct: 500 YEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIP 533

BLAST of CsGy1G024210 vs. ExPASy Swiss-Prot
Match: Q9SVG4 (Berberine bridge enzyme-like 19 OS=Arabidopsis thaliana OX=3702 GN=At4g20830 PE=1 SV=2)

HSP 1 Score: 572.4 bits (1474), Expect = 5.4e-162
Identity = 290/524 (55.34%), Postives = 376/524 (71.76%), Query Frame = 0

Query: 13  FSFLVFFS------SASSSAPENFFQCLL-RHSPPSYPIAPAIFTPNNASYSSVLETYIR 72
           F FL+F S      S S+S   +F +C   +   P   I   +F+  N ++SSVL  YIR
Sbjct: 16  FFFLLFLSLPLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIR 75

Query: 73  NLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFF 132
           N RFNTS+T KP +I+T   ESHV AA+ C++  N  +KIRSGGHDY+G+SY+SD  PFF
Sbjct: 76  NARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISD-KPFF 135

Query: 133 ILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGGHFGGG 192
           ILDM N+R+++VD  + +AW+  GATLGEVYYR+ E ++ +GFPAGVCPTVGVGGH  GG
Sbjct: 136 ILDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGG 195

Query: 193 GYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRM 252
           GYGNM+RK+GLSVD + DAKI+DVNG++LDRKAMGEDLFWAI GGGG S+GVV+ YK+++
Sbjct: 196 GYGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKL 255

Query: 253 VPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKTLRA 312
           VPVP  VTVFRV++ ++ +   ++V +WQ V    D +LF+R+    V  T K  KT+RA
Sbjct: 256 VPVPSVVTVFRVEQYMD-SGAVDMVHKWQSVGPKTDPNLFMRMLIQPV--TRKKVKTVRA 315

Query: 313 TFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVE--ALLSR 372
           + +ALFLG ++ ++ +++  FP+LGLKK +C EM+W QS L+W N    T V+    L R
Sbjct: 316 SVVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDR 375

Query: 373 TPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPH 432
                +  KRKSDYV T IPK+G+  ++KKMIEL K  L FNPYGG+MAE+  NA PFPH
Sbjct: 376 NLDTSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPH 435

Query: 433 RAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVNH 492
           R   L+KIQY+ NW E   +  K +++  + LY FMT FVSKNPR+++ NYRD+DIGVN 
Sbjct: 436 R-NKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVND 495

Query: 493 NGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIP 528
           +G NSY EG VYG KYF  NFDRLVKIKT VDP NFFRNEQSIP
Sbjct: 496 HGANSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIP 534

BLAST of CsGy1G024210 vs. ExPASy Swiss-Prot
Match: Q9SA99 (Berberine bridge enzyme-like 2 OS=Arabidopsis thaliana OX=3702 GN=At1g11770 PE=2 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 5.9e-161
Identity = 287/530 (54.15%), Postives = 371/530 (70.00%), Query Frame = 0

Query: 8   ILLLSFSFLVFFSSASSSAPE----NFFQCLLRHS-PPSYPIAPAIFTPNNASYSSVLET 67
           ++L   S  +  S A    PE    NF QC    +  P   +A  +     A+++ VL  
Sbjct: 6   LILFLISSFISTSLAVEPPPETIYQNFLQCFTNQTKAPPNSLADVVLPKTAAAFTPVLRA 65

Query: 68  YIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDVV 127
           YIRN RFNT+ TPKP +++ A  ESHV+AA++C +  N+Q+K RSGGHDYEGVSY+S  V
Sbjct: 66  YIRNARFNTTATPKPAIVIAARSESHVQAAVICTKSLNIQLKTRSGGHDYEGVSYISH-V 125

Query: 128 PFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGGHF 187
           PFF+LDM NLR ITVD  T++AWV  GATLGEVYYR+ E  KS+GFPAGVCPTVG GGH 
Sbjct: 126 PFFVLDMSNLRNITVDPATESAWVGAGATLGEVYYRIWEKTKSHGFPAGVCPTVGAGGHI 185

Query: 188 GGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYK 247
            GGGYGNM+RKYGLSVD + DAKI+DVNG++LDRK MGED+FWAI GGGG+SFGV++A+K
Sbjct: 186 SGGGYGNMIRKYGLSVDYVTDAKIVDVNGQVLDRKGMGEDMFWAINGGGGASFGVILAFK 245

Query: 248 IRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKT 307
           I++VPVP TVTVFRV++ L +N  TE+V +WQ VA   D  LF+R+    V  T    +T
Sbjct: 246 IKLVPVPPTVTVFRVEKNLVEN-ATEMVHKWQFVAPKTDPGLFMRLLLQPV--TRNKMQT 305

Query: 308 LRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSV--EAL 367
           +RA+ +ALFLGD   ++ ++   FP+LGLKK +C EM+W+QSV++W N    T +  E L
Sbjct: 306 VRASVVALFLGDQNTVMSMLTKEFPELGLKKENCTEMTWIQSVMWWANNDNATQIKPEIL 365

Query: 368 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 427
           L R P + T  KRKSD+V+  I K+GL+ ++KKMIE+ K  L FNPYGG M+ + +  TP
Sbjct: 366 LDRNPDMATFGKRKSDFVEKEITKDGLDFLFKKMIEVGKIGLVFNPYGGIMSTVATTKTP 425

Query: 428 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 487
           FPHR   L+KIQ++ NW + G +    F+   +  Y +M PFV+KNPR  ++NYRDLDIG
Sbjct: 426 FPHRK-KLYKIQHSMNWKDPGTEAETSFLQKAKSFYSYMAPFVTKNPRHTYINYRDLDIG 485

Query: 488 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP 531
           VN  G NSY    V+G  YF  NFDRLVK+KT VDP NFFR+EQSIP  P
Sbjct: 486 VNTPGPNSYRVAEVFGRMYFGENFDRLVKVKTAVDPQNFFRDEQSIPTLP 530

BLAST of CsGy1G024210 vs. ExPASy Swiss-Prot
Match: F4HV09 (Berberine bridge enzyme-like 14 OS=Arabidopsis thaliana OX=3702 GN=At1g34575 PE=3 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 4.7e-158
Identity = 279/528 (52.84%), Postives = 383/528 (72.54%), Query Frame = 0

Query: 6   TSILLLSFSFLVFFSSASSSAP---ENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVLET 65
           T+  L+   FL+   ++ ++ P   ++F QC+     PS PI    +T  N ++ ++L  
Sbjct: 5   TTQTLIFTVFLLLIPTSFAAPPKLKDSFTQCVTVFK-PSVPIQNFTYTQQNPNFLTILNN 64

Query: 66  YIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDVV 125
           Y+RNLR+   TT KP  I+ A H +H++A I CA+   LQ++IRSGGHDY+G+SY+S  V
Sbjct: 65  YVRNLRYFNGTTRKPVAIVAAAHFTHIQATINCAKKLGLQLRIRSGGHDYDGMSYLS-TV 124

Query: 126 PFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVA-ENNKSYGFPAGVCPTVGVGGH 185
            F +LDMFNLR I +D K  TAWVQ+GATLGE+YY VA ++N   GFPAG+CP +G GGH
Sbjct: 125 DFVVLDMFNLRAIEIDPKLDTAWVQSGATLGEIYYNVANKSNNLRGFPAGICPGLGAGGH 184

Query: 186 FGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 245
           F GGGYGNMMRKYGLS+D IIDAKI+D N ++LDR +MGEDLFWA+ GGG +SF VV+A+
Sbjct: 185 FSGGGYGNMMRKYGLSIDNIIDAKIVDANARVLDRSSMGEDLFWALRGGGAASFCVVLAW 244

Query: 246 KIRMVPVPETVTVFRVQRTLEQNDL-TEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 305
           KI++VPVPE VTVF V+    +  + T++  +WQ++A  IDNDLFIR+T    N      
Sbjct: 245 KIKLVPVPEKVTVFNVETIGNRGVIPTDLAAKWQEIADKIDNDLFIRLTLSSSN------ 304

Query: 306 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTS-VEA 365
           KT++A+F+ ++LG+SE+LL ++N  FP+LGL K++CIEM W++SVLFW + P GT+    
Sbjct: 305 KTVKASFMGMYLGNSEKLLEIMNAKFPELGLNKTECIEMKWIESVLFWLSIPPGTAPTSV 364

Query: 366 LLSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNAT 425
           +L+R PQ   +LKRKSDYV+ PI K GL  I+K + E E   + +NPYGGRM+EIP+  T
Sbjct: 365 MLNRIPQKQIYLKRKSDYVQKPISKPGLESIFKILSENENVSMAWNPYGGRMSEIPATET 424

Query: 426 PFPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDI 485
            FPHRAGN++KIQY++NW   G + A   +  T ++++ M+P+VSKNPR AFLNYRD+DI
Sbjct: 425 AFPHRAGNMFKIQYSSNWFVPGEEAASDCLSQTERVFEAMSPYVSKNPREAFLNYRDIDI 484

Query: 486 GVNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIP 528
           G N N  ++Y EG+VYG+KYF+ NF+RLV++KT+VDP N FR EQSIP
Sbjct: 485 GKNLN--STYEEGKVYGVKYFKNNFERLVQVKTRVDPDNIFRYEQSIP 522

BLAST of CsGy1G024210 vs. NCBI nr
Match: XP_004135723.1 (berberine bridge enzyme-like 8 [Cucumis sativus] >KAE8653304.1 hypothetical protein Csa_023313 [Cucumis sativus])

HSP 1 Score: 1080 bits (2793), Expect = 0.0
Identity = 533/533 (100.00%), Postives = 533/533 (100.00%), Query Frame = 0

Query: 1   MEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           MEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL
Sbjct: 1   MEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120

Query: 121 VVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGG 180
           VVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGG
Sbjct: 121 VVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGG 180

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300
           YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK
Sbjct: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300

Query: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360
           KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL
Sbjct: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360

Query: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420

Query: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHVW 533
           VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHVW
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHVW 533

BLAST of CsGy1G024210 vs. NCBI nr
Match: XP_008445642.1 (PREDICTED: reticuline oxidase-like protein [Cucumis melo] >KAA0047124.1 reticuline oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1034 bits (2674), Expect = 0.0
Identity = 510/532 (95.86%), Postives = 521/532 (97.93%), Query Frame = 0

Query: 1   MEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           M+ILR+SIL LSFSFLVFFSSASSSAPENF QCLLRHSPPSYPIAPAIFTPNNASYSSVL
Sbjct: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIHNLQMKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120

Query: 121 VVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGG 180
           VVPFF+LDMFNLREITVDAKT+TAWVQTGATLGEVYYRVAE NKSYGFPAGVCPTVGVGG
Sbjct: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRK+GLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300
           YKIRMV VPE VTVFRVQRTLEQN+LTEIVDEWQQVAHVIDNDLFIRVTFDVVNGT+K K
Sbjct: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300

Query: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360
           KTLRATFIALFLGDS RLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL
Sbjct: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360

Query: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVK PIPKEGL+KIWKKMIELEKPM+TFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLDKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420

Query: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNW EEG +EAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 532
           VNHNGKNSYYEGRVYGIKYFEGNF+RLVKIKTKVDPHNFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of CsGy1G024210 vs. NCBI nr
Match: XP_038895093.1 (berberine bridge enzyme-like 8 [Benincasa hispida])

HSP 1 Score: 1019 bits (2636), Expect = 0.0
Identity = 501/532 (94.17%), Postives = 518/532 (97.37%), Query Frame = 0

Query: 1   MEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           MEILR+SILLLSFS+LVFFSSA+S APENFFQCLLRHSPPSYPIAPAIFTPNN S+SSVL
Sbjct: 1   MEILRSSILLLSFSYLVFFSSATS-APENFFQCLLRHSPPSYPIAPAIFTPNNVSFSSVL 60

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIH+LQMKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSD 120

Query: 121 VVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGG 180
           V+PFFILDMFNLR+ITVD KTKTAWVQTGATLGEVYYR+AE NK YGFPAGVCPTVGVGG
Sbjct: 121 VLPFFILDMFNLRDITVDVKTKTAWVQTGATLGEVYYRIAEKNKFYGFPAGVCPTVGVGG 180

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRKYGLSVDQIIDAKI+DVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300
           YKIRMV VPETVTVFRVQRTLEQNDLTEIVDEWQ+VAHVIDNDLFIRVTFDV+NGT KGK
Sbjct: 241 YKIRMVRVPETVTVFRVQRTLEQNDLTEIVDEWQRVAHVIDNDLFIRVTFDVINGTEKGK 300

Query: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360
           KTLRATF+A+FLGDSERLL VI  SFPKLGLKKSDC+EMSWLQSVLFWTNFPLGTSV+AL
Sbjct: 301 KTLRATFLAMFLGDSERLLSVIKKSFPKLGLKKSDCLEMSWLQSVLFWTNFPLGTSVDAL 360

Query: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420

Query: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNW EEG +EAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWDEEGTQEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 532
           VNHNGKNSYYEGRVYGIKYFEGNF+RLVKIKTKVDP NFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV 531

BLAST of CsGy1G024210 vs. NCBI nr
Match: XP_022994779.1 (berberine bridge enzyme-like 8 [Cucurbita maxima])

HSP 1 Score: 960 bits (2481), Expect = 0.0
Identity = 473/532 (88.91%), Postives = 500/532 (93.98%), Query Frame = 0

Query: 1   MEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           MEI R SI LLSFS L+FF+SA++ APENF QCLLRHSPPSYPIAPAIFT NNASYSSVL
Sbjct: 3   MEISRFSIFLLSFSALLFFASATT-APENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVL 62

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIH+LQMKIRSGGHDYEGVSYVSD
Sbjct: 63  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSD 122

Query: 121 VVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGG 180
           VVPFF+LDMFNLR+I+VD KT+TAWVQTGATLGEVYYR+AE  K +GFPAGVCPTVGVGG
Sbjct: 123 VVPFFVLDMFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGG 182

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRKYGLSVDQIIDAKI+DV G LLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 183 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVYGNLLDRKAMGEDLFWAIIGGGGSSFGVVVA 242

Query: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300
           YKIR V VPETVTVFRV RTLE ND+TEIVD+WQ+VAHVID DLFIRVTFDVVNG+  GK
Sbjct: 243 YKIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSRSGK 302

Query: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360
           KTLRATFIALFLGDSERLL V+N SFPKLGLKKSDC+EMSWLQSVLFWTNFP GTSV+ L
Sbjct: 303 KTLRATFIALFLGDSERLLSVMNKSFPKLGLKKSDCLEMSWLQSVLFWTNFPQGTSVDEL 362

Query: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVKT IPKE LN+IWKKMIELEKP +TFNPYGGRMAEIPSNATP
Sbjct: 363 LSRTPQVLTHLKRKSDYVKTLIPKEKLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATP 422

Query: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNW E+G ++A HFIDLTRKLYKFMTPFV+KNPRTAFLNYRDLDIG
Sbjct: 423 FPHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIG 482

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 532
           VNHNGK SYYEGRV+GIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP+V
Sbjct: 483 VNHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPYV 533

BLAST of CsGy1G024210 vs. NCBI nr
Match: XP_023542409.1 (berberine bridge enzyme-like 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 959 bits (2480), Expect = 0.0
Identity = 473/530 (89.25%), Postives = 498/530 (93.96%), Query Frame = 0

Query: 1   MEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           MEI R S  LLSFS L+FF+SA++ APENF QCLLRHSPPSYPIAPAIFT NNASYSSVL
Sbjct: 3   MEISRFSSFLLSFSALLFFASATT-APENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVL 62

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIH+LQMKIRSGGHDYEGVSYVSD
Sbjct: 63  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSD 122

Query: 121 VVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGG 180
           VVPFF+LDMFNLR+I+VD KTKTAWVQTGATLGEVYYR+AE  K +GFPAGVCPTVGVGG
Sbjct: 123 VVPFFVLDMFNLRDISVDVKTKTAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGG 182

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRKYGLSVDQIIDAKI+DVNG LLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 183 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVNGNLLDRKAMGEDLFWAIIGGGGSSFGVVVA 242

Query: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300
           YKIR V VPETVTVFRV RTLE ND+TEIVD+WQ+VAHVID DLFIRVTFDVVNG+  GK
Sbjct: 243 YKIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSKSGK 302

Query: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360
           KTLRATFIALFLGDSERLL V+N SFPKLGLKKSDC+EMSWLQSVLFWTNFP GTSV+ L
Sbjct: 303 KTLRATFIALFLGDSERLLSVMNKSFPKLGLKKSDCLEMSWLQSVLFWTNFPQGTSVDEL 362

Query: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVKT IPKE LN+IWKKMIELEKP +TFNPYGGRMAEIPSNATP
Sbjct: 363 LSRTPQVLTHLKRKSDYVKTLIPKEKLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATP 422

Query: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNW E+G ++A HFIDLTRKLYKFMTPFV+KNPRTAFLNYRDLDIG
Sbjct: 423 FPHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIG 482

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP 530
           VNHNGK SYYEGRV+GIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP
Sbjct: 483 VNHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP 531

BLAST of CsGy1G024210 vs. ExPASy TrEMBL
Match: A0A5A7TVZ2 (Reticuline oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold466G001070 PE=3 SV=1)

HSP 1 Score: 1034 bits (2674), Expect = 0.0
Identity = 510/532 (95.86%), Postives = 521/532 (97.93%), Query Frame = 0

Query: 1   MEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           M+ILR+SIL LSFSFLVFFSSASSSAPENF QCLLRHSPPSYPIAPAIFTPNNASYSSVL
Sbjct: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIHNLQMKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120

Query: 121 VVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGG 180
           VVPFF+LDMFNLREITVDAKT+TAWVQTGATLGEVYYRVAE NKSYGFPAGVCPTVGVGG
Sbjct: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRK+GLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300
           YKIRMV VPE VTVFRVQRTLEQN+LTEIVDEWQQVAHVIDNDLFIRVTFDVVNGT+K K
Sbjct: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300

Query: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360
           KTLRATFIALFLGDS RLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL
Sbjct: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360

Query: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVK PIPKEGL+KIWKKMIELEKPM+TFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLDKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420

Query: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNW EEG +EAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 532
           VNHNGKNSYYEGRVYGIKYFEGNF+RLVKIKTKVDPHNFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of CsGy1G024210 vs. ExPASy TrEMBL
Match: A0A1S3BE21 (reticuline oxidase-like protein OS=Cucumis melo OX=3656 GN=LOC103488597 PE=3 SV=1)

HSP 1 Score: 1034 bits (2674), Expect = 0.0
Identity = 510/532 (95.86%), Postives = 521/532 (97.93%), Query Frame = 0

Query: 1   MEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           M+ILR+SIL LSFSFLVFFSSASSSAPENF QCLLRHSPPSYPIAPAIFTPNNASYSSVL
Sbjct: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIHNLQMKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120

Query: 121 VVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGG 180
           VVPFF+LDMFNLREITVDAKT+TAWVQTGATLGEVYYRVAE NKSYGFPAGVCPTVGVGG
Sbjct: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRK+GLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300
           YKIRMV VPE VTVFRVQRTLEQN+LTEIVDEWQQVAHVIDNDLFIRVTFDVVNGT+K K
Sbjct: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300

Query: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360
           KTLRATFIALFLGDS RLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL
Sbjct: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360

Query: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVK PIPKEGL+KIWKKMIELEKPM+TFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLDKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420

Query: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNW EEG +EAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 532
           VNHNGKNSYYEGRVYGIKYFEGNF+RLVKIKTKVDPHNFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of CsGy1G024210 vs. ExPASy TrEMBL
Match: A0A6J1K3U3 (berberine bridge enzyme-like 8 OS=Cucurbita maxima OX=3661 GN=LOC111490407 PE=3 SV=1)

HSP 1 Score: 960 bits (2481), Expect = 0.0
Identity = 473/532 (88.91%), Postives = 500/532 (93.98%), Query Frame = 0

Query: 1   MEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           MEI R SI LLSFS L+FF+SA++ APENF QCLLRHSPPSYPIAPAIFT NNASYSSVL
Sbjct: 3   MEISRFSIFLLSFSALLFFASATT-APENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVL 62

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIH+LQMKIRSGGHDYEGVSYVSD
Sbjct: 63  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSD 122

Query: 121 VVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGG 180
           VVPFF+LDMFNLR+I+VD KT+TAWVQTGATLGEVYYR+AE  K +GFPAGVCPTVGVGG
Sbjct: 123 VVPFFVLDMFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGG 182

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRKYGLSVDQIIDAKI+DV G LLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 183 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVYGNLLDRKAMGEDLFWAIIGGGGSSFGVVVA 242

Query: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300
           YKIR V VPETVTVFRV RTLE ND+TEIVD+WQ+VAHVID DLFIRVTFDVVNG+  GK
Sbjct: 243 YKIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSRSGK 302

Query: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360
           KTLRATFIALFLGDSERLL V+N SFPKLGLKKSDC+EMSWLQSVLFWTNFP GTSV+ L
Sbjct: 303 KTLRATFIALFLGDSERLLSVMNKSFPKLGLKKSDCLEMSWLQSVLFWTNFPQGTSVDEL 362

Query: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVKT IPKE LN+IWKKMIELEKP +TFNPYGGRMAEIPSNATP
Sbjct: 363 LSRTPQVLTHLKRKSDYVKTLIPKEKLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATP 422

Query: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNW E+G ++A HFIDLTRKLYKFMTPFV+KNPRTAFLNYRDLDIG
Sbjct: 423 FPHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIG 482

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 532
           VNHNGK SYYEGRV+GIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP+V
Sbjct: 483 VNHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPYV 533

BLAST of CsGy1G024210 vs. ExPASy TrEMBL
Match: A0A6J1GSQ1 (berberine bridge enzyme-like 8 OS=Cucurbita moschata OX=3662 GN=LOC111456785 PE=3 SV=1)

HSP 1 Score: 955 bits (2468), Expect = 0.0
Identity = 470/532 (88.35%), Postives = 499/532 (93.80%), Query Frame = 0

Query: 1   MEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60
           M+I R S  LLSFS L+FF+SA++ APENF QCLLRHSPPSYPIAPAIFT NNASYSSVL
Sbjct: 3   MDISRFSSFLLSFSALLFFASATT-APENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVL 62

Query: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           ETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIH+LQMKIRSGGHDYEGVSYVSD
Sbjct: 63  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSD 122

Query: 121 VVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGG 180
           VVPFF+LDMFNLR+I+VD KT+TAWVQTGATLGEVYYR+AE  K +GFPAGVCPTVGVGG
Sbjct: 123 VVPFFVLDMFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGG 182

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240
           HFGGGGYGNMMRKYGLSVDQIIDAKI+DVNG LLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 183 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVNGNLLDRKAMGEDLFWAIIGGGGSSFGVVVA 242

Query: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300
           YKIR V VPETVTVFRV RTLE ND+TEIVD+WQ+VAHVID DLFIRVTFDVVNG+  GK
Sbjct: 243 YKIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSRSGK 302

Query: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360
           KTLRATFIALFLGDSERLL V+N SFPKLGLKK+DC+EMSWLQSVLFWTNFP GTSV+ L
Sbjct: 303 KTLRATFIALFLGDSERLLSVMNKSFPKLGLKKADCLEMSWLQSVLFWTNFPQGTSVDEL 362

Query: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420
           LSRTPQVLTHLKRKSDYVKT IPKE LN+IWKKMIELEKP +TFNPYGGRMAEIPSNATP
Sbjct: 363 LSRTPQVLTHLKRKSDYVKTLIPKENLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATP 422

Query: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNW E+G ++A HFIDLTRKLYKFMTPFV+KNPRTAFLNYRDLDIG
Sbjct: 423 FPHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIG 482

Query: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 532
           VNHNGK SYYEGRV+GIKYFEGNFDRLVKIKTKVDP NFFRNEQSIPRFP+V
Sbjct: 483 VNHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPLNFFRNEQSIPRFPYV 533

BLAST of CsGy1G024210 vs. ExPASy TrEMBL
Match: A0A6J1DGF3 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020214 PE=3 SV=1)

HSP 1 Score: 914 bits (2362), Expect = 0.0
Identity = 448/531 (84.37%), Postives = 493/531 (92.84%), Query Frame = 0

Query: 3   ILRTSILL-LSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVLE 62
           ILR   LL L FS L F S+ ++++PENF +CLLR SPPS+PI+PAIFTP+NAS+SSVL+
Sbjct: 5   ILRPFFLLSLLFSLLFFASATTTASPENFLKCLLRRSPPSHPISPAIFTPDNASFSSVLQ 64

Query: 63  TYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDV 122
           TYIRNLRFNTS+TPKPFLILTALHESHVK AILCARIHNLQMKIRSGGHDYEGVSYVSDV
Sbjct: 65  TYIRNLRFNTSSTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSDV 124

Query: 123 VPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGGH 182
           VPFF+LDMFNLR ITVD +T+TAWV+TGATLGEVYYR+AE +K +GFPAGVCPTVGVGGH
Sbjct: 125 VPFFVLDMFNLRAITVDVETETAWVETGATLGEVYYRIAEKSKLHGFPAGVCPTVGVGGH 184

Query: 183 FGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 242
           FGGGGYGNMMRKYGLSVDQIIDAK++DVNGKLLDRK+MGEDLFWAI GGGGSSFG VVAY
Sbjct: 185 FGGGGYGNMMRKYGLSVDQIIDAKMVDVNGKLLDRKSMGEDLFWAITGGGGSSFGXVVAY 244

Query: 243 KIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKK 302
           KI+ V VPETVTVF+V+R L +N +TEIVDEWQ+VAH IDNDLFIRVTFDVVNGT  GKK
Sbjct: 245 KIQTVRVPETVTVFKVERRLAEN-VTEIVDEWQRVAHEIDNDLFIRVTFDVVNGTENGKK 304

Query: 303 TLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEALL 362
           TLRATFIALFLGDSERLL +++ SFPKLGLK+SDC++MSWL+SVLFWTNFPLGTSV+ALL
Sbjct: 305 TLRATFIALFLGDSERLLSIMDKSFPKLGLKQSDCLQMSWLESVLFWTNFPLGTSVDALL 364

Query: 363 SRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPF 422
           SR PQVLTHLKRKSDYVKT IPKEGLN+IWKKMIELEKPMLTFNPYGGRMAEI S+ TPF
Sbjct: 365 SRKPQVLTHLKRKSDYVKTLIPKEGLNQIWKKMIELEKPMLTFNPYGGRMAEIASSKTPF 424

Query: 423 PHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGV 482
           PHRAGNLWKIQYATNW EEG +EA HFI+LTRKLY FMTPFVSKNPRTAFLNYRDLDIGV
Sbjct: 425 PHRAGNLWKIQYATNWDEEGVEEADHFINLTRKLYNFMTPFVSKNPRTAFLNYRDLDIGV 484

Query: 483 NHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 532
           NHNGKNSY EGRV+G+KYFEGNFDRLVKIKT+VDP+NFFRNEQSIPR P+V
Sbjct: 485 NHNGKNSYNEGRVFGVKYFEGNFDRLVKIKTRVDPNNFFRNEQSIPRLPYV 534

BLAST of CsGy1G024210 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 686.4 bits (1770), Expect = 1.8e-197
Identity = 339/526 (64.45%), Postives = 411/526 (78.14%), Query Frame = 0

Query: 8   ILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVLETYIRNL 67
           IL+L F   ++ SS+SS+  E F QCL  +S P +PI+PAIF   N SYSSVL+  IRNL
Sbjct: 6   ILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIRNL 65

Query: 68  RFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVS-DVVPFFI 127
           RFNT++TPKPFLI+ A HESHV+AAI C + HNLQMKIRSGGHDY+G+SYV+    PFF+
Sbjct: 66  RFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFV 125

Query: 128 LDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGGHFGGGG 187
           LDMFNLR + VD  +KTAWVQTGA LGEVYY + E +K+  +PAG+CPTVGVGGH  GGG
Sbjct: 126 LDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGG 185

Query: 188 YGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRMV 247
           YGNMMRKYGL+VD  IDA+++DVNGK+LDRK MGEDL+WAI GGGG S+GVV+AYKI +V
Sbjct: 186 YGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLV 245

Query: 248 PVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKTLRAT 307
            VPE VTVFR+ RTLEQN  T+I+  WQQVA  + ++LFIR   DVVNGT   +KT+R T
Sbjct: 246 EVPENVTVFRISRTLEQN-ATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRTT 305

Query: 308 FIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEALLSRTPQ 367
           FIA+FLGD+  LL ++N  FP+LGL +SDC E SW+QSVLFWTN  +G+S E LL +  Q
Sbjct: 306 FIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSS-ETLLLQRNQ 365

Query: 368 VLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPHRAG 427
            + +LKRKSDYV+ PI + GL  IWKKMIELE P + FNPYGG M  I S  TPFP+RAG
Sbjct: 366 PVNYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAG 425

Query: 428 NLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVN-HNG 487
           NLWKIQY  NW +E   +   +++LTRKLY+FMTPFVSKNPR +F NYRD+D+G+N HNG
Sbjct: 426 NLWKIQYGANWRDETLTD--RYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNG 485

Query: 488 K-NSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP 531
           K +SY EG+ YG KYF GNF+RLVKIKT+VD  NFFRNEQSIP  P
Sbjct: 486 KISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVLP 527

BLAST of CsGy1G024210 vs. TAIR 10
Match: AT4G20840.1 (FAD-binding Berberine family protein )

HSP 1 Score: 581.6 bits (1498), Expect = 6.3e-166
Identity = 296/519 (57.03%), Postives = 374/519 (72.06%), Query Frame = 0

Query: 16  LVFFSSA----SSSAPENFFQCLL-RHSPPSYPIAPAIFTPNNASYSSVLETYIRNLRFN 75
           L FFSSA    S S  E+F QC   +   P   I   +F+  N S+SSVL  YIRN RFN
Sbjct: 20  LPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSSVLRAYIRNGRFN 79

Query: 76  TSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFFILDMF 135
           TS+TPKP +I+T   + HV AA+ C++  N  +KIRSGGHDYEG+SY+SD  PFFILDM 
Sbjct: 80  TSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYISD-KPFFILDMS 139

Query: 136 NLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGGHFGGGGYGNM 195
           NLR+++VD   ++AW+  GATLGEVYYR+ E +K +GFPAGVCPTVGVGGH  GGGYGNM
Sbjct: 140 NLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGVGGHISGGGYGNM 199

Query: 196 MRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRMVPVPE 255
           +RK+GLSVD +IDAKI+DVNG++LDRK+MGEDLFWAI GGGG+SFGVV+ YK+++VPVPE
Sbjct: 200 LRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVLGYKVKLVPVPE 259

Query: 256 TVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKTLRATFIAL 315
           TVTVFRV++ ++ +   ++V +WQ V    D +LF+R+    V  T K  KT+RAT +AL
Sbjct: 260 TVTVFRVEKYMD-SGAVDMVHKWQSVGPKTDRNLFLRMLIQPV--TRKKVKTVRATVVAL 319

Query: 316 FLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVE--ALLSRTPQVL 375
           FLG +E ++ ++   FP+L LKK +C EM+W QS L+W N    T ++    L R     
Sbjct: 320 FLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDPKVFLDRNLDRA 379

Query: 376 THLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPHRAGNL 435
              KRKSDYV + IP++G+  ++KKM EL K  L FNPYGG+MAE+  NATPFPHR+  L
Sbjct: 380 NFGKRKSDYVASEIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTVNATPFPHRS-KL 439

Query: 436 WKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVNHNGKNS 495
           +KIQY+  W E   +  K F++    LY FMT FVSKNPR A+LNYRD+DIGVN +G NS
Sbjct: 440 FKIQYSVTWQENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRDVDIGVNDHGTNS 499

Query: 496 YYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIP 528
           Y EG VYG KYF  NFDRLVK+KT  DP NFFRNEQSIP
Sbjct: 500 YEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIP 533

BLAST of CsGy1G024210 vs. TAIR 10
Match: AT4G20830.1 (FAD-binding Berberine family protein )

HSP 1 Score: 572.4 bits (1474), Expect = 3.8e-163
Identity = 290/524 (55.34%), Postives = 376/524 (71.76%), Query Frame = 0

Query: 13  FSFLVFFS------SASSSAPENFFQCLL-RHSPPSYPIAPAIFTPNNASYSSVLETYIR 72
           F FL+F S      S S+S   +F +C   +   P   I   +F+  N ++SSVL  YIR
Sbjct: 16  FFFLLFLSLPLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIR 75

Query: 73  NLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFF 132
           N RFNTS+T KP +I+T   ESHV AA+ C++  N  +KIRSGGHDY+G+SY+SD  PFF
Sbjct: 76  NARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISD-KPFF 135

Query: 133 ILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGGHFGGG 192
           ILDM N+R+++VD  + +AW+  GATLGEVYYR+ E ++ +GFPAGVCPTVGVGGH  GG
Sbjct: 136 ILDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGG 195

Query: 193 GYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRM 252
           GYGNM+RK+GLSVD + DAKI+DVNG++LDRKAMGEDLFWAI GGGG S+GVV+ YK+++
Sbjct: 196 GYGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKL 255

Query: 253 VPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKTLRA 312
           VPVP  VTVFRV++ ++ +   ++V +WQ V    D +LF+R+    V  T K  KT+RA
Sbjct: 256 VPVPSVVTVFRVEQYMD-SGAVDMVHKWQSVGPKTDPNLFMRMLIQPV--TRKKVKTVRA 315

Query: 313 TFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVE--ALLSR 372
           + +ALFLG ++ ++ +++  FP+LGLKK +C EM+W QS L+W N    T V+    L R
Sbjct: 316 SVVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDR 375

Query: 373 TPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPH 432
                +  KRKSDYV T IPK+G+  ++KKMIEL K  L FNPYGG+MAE+  NA PFPH
Sbjct: 376 NLDTSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPH 435

Query: 433 RAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVNH 492
           R   L+KIQY+ NW E   +  K +++  + LY FMT FVSKNPR+++ NYRD+DIGVN 
Sbjct: 436 R-NKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVND 495

Query: 493 NGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIP 528
           +G NSY EG VYG KYF  NFDRLVKIKT VDP NFFRNEQSIP
Sbjct: 496 HGANSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIP 534

BLAST of CsGy1G024210 vs. TAIR 10
Match: AT4G20830.2 (FAD-binding Berberine family protein )

HSP 1 Score: 572.4 bits (1474), Expect = 3.8e-163
Identity = 290/524 (55.34%), Postives = 376/524 (71.76%), Query Frame = 0

Query: 13  FSFLVFFS------SASSSAPENFFQCLL-RHSPPSYPIAPAIFTPNNASYSSVLETYIR 72
           F FL+F S      S S+S   +F +C   +   P   I   +F+  N ++SSVL  YIR
Sbjct: 16  FFFLLFLSLPLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIR 75

Query: 73  NLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFF 132
           N RFNTS+T KP +I+T   ESHV AA+ C++  N  +KIRSGGHDY+G+SY+SD  PFF
Sbjct: 76  NARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISD-KPFF 135

Query: 133 ILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGGHFGGG 192
           ILDM N+R+++VD  + +AW+  GATLGEVYYR+ E ++ +GFPAGVCPTVGVGGH  GG
Sbjct: 136 ILDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGG 195

Query: 193 GYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRM 252
           GYGNM+RK+GLSVD + DAKI+DVNG++LDRKAMGEDLFWAI GGGG S+GVV+ YK+++
Sbjct: 196 GYGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKL 255

Query: 253 VPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKTLRA 312
           VPVP  VTVFRV++ ++ +   ++V +WQ V    D +LF+R+    V  T K  KT+RA
Sbjct: 256 VPVPSVVTVFRVEQYMD-SGAVDMVHKWQSVGPKTDPNLFMRMLIQPV--TRKKVKTVRA 315

Query: 313 TFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVE--ALLSR 372
           + +ALFLG ++ ++ +++  FP+LGLKK +C EM+W QS L+W N    T V+    L R
Sbjct: 316 SVVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDR 375

Query: 373 TPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPH 432
                +  KRKSDYV T IPK+G+  ++KKMIEL K  L FNPYGG+MAE+  NA PFPH
Sbjct: 376 NLDTSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPH 435

Query: 433 RAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVNH 492
           R   L+KIQY+ NW E   +  K +++  + LY FMT FVSKNPR+++ NYRD+DIGVN 
Sbjct: 436 R-NKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVND 495

Query: 493 NGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIP 528
           +G NSY EG VYG KYF  NFDRLVKIKT VDP NFFRNEQSIP
Sbjct: 496 HGANSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIP 534

BLAST of CsGy1G024210 vs. TAIR 10
Match: AT1G11770.1 (FAD-binding Berberine family protein )

HSP 1 Score: 568.9 bits (1465), Expect = 4.2e-162
Identity = 287/530 (54.15%), Postives = 371/530 (70.00%), Query Frame = 0

Query: 8   ILLLSFSFLVFFSSASSSAPE----NFFQCLLRHS-PPSYPIAPAIFTPNNASYSSVLET 67
           ++L   S  +  S A    PE    NF QC    +  P   +A  +     A+++ VL  
Sbjct: 6   LILFLISSFISTSLAVEPPPETIYQNFLQCFTNQTKAPPNSLADVVLPKTAAAFTPVLRA 65

Query: 68  YIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDVV 127
           YIRN RFNT+ TPKP +++ A  ESHV+AA++C +  N+Q+K RSGGHDYEGVSY+S  V
Sbjct: 66  YIRNARFNTTATPKPAIVIAARSESHVQAAVICTKSLNIQLKTRSGGHDYEGVSYISH-V 125

Query: 128 PFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGGHF 187
           PFF+LDM NLR ITVD  T++AWV  GATLGEVYYR+ E  KS+GFPAGVCPTVG GGH 
Sbjct: 126 PFFVLDMSNLRNITVDPATESAWVGAGATLGEVYYRIWEKTKSHGFPAGVCPTVGAGGHI 185

Query: 188 GGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYK 247
            GGGYGNM+RKYGLSVD + DAKI+DVNG++LDRK MGED+FWAI GGGG+SFGV++A+K
Sbjct: 186 SGGGYGNMIRKYGLSVDYVTDAKIVDVNGQVLDRKGMGEDMFWAINGGGGASFGVILAFK 245

Query: 248 IRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKT 307
           I++VPVP TVTVFRV++ L +N  TE+V +WQ VA   D  LF+R+    V  T    +T
Sbjct: 246 IKLVPVPPTVTVFRVEKNLVEN-ATEMVHKWQFVAPKTDPGLFMRLLLQPV--TRNKMQT 305

Query: 308 LRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSV--EAL 367
           +RA+ +ALFLGD   ++ ++   FP+LGLKK +C EM+W+QSV++W N    T +  E L
Sbjct: 306 VRASVVALFLGDQNTVMSMLTKEFPELGLKKENCTEMTWIQSVMWWANNDNATQIKPEIL 365

Query: 368 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 427
           L R P + T  KRKSD+V+  I K+GL+ ++KKMIE+ K  L FNPYGG M+ + +  TP
Sbjct: 366 LDRNPDMATFGKRKSDFVEKEITKDGLDFLFKKMIEVGKIGLVFNPYGGIMSTVATTKTP 425

Query: 428 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 487
           FPHR   L+KIQ++ NW + G +    F+   +  Y +M PFV+KNPR  ++NYRDLDIG
Sbjct: 426 FPHRK-KLYKIQHSMNWKDPGTEAETSFLQKAKSFYSYMAPFVTKNPRHTYINYRDLDIG 485

Query: 488 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP 531
           VN  G NSY    V+G  YF  NFDRLVK+KT VDP NFFR+EQSIP  P
Sbjct: 486 VNTPGPNSYRVAEVFGRMYFGENFDRLVKVKTAVDPQNFFRDEQSIPTLP 530

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SA852.5e-19664.45Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2... [more]
Q9SVG38.8e-16557.03Berberine bridge enzyme-like 21 OS=Arabidopsis thaliana OX=3702 GN=At4g20840 PE=... [more]
Q9SVG45.4e-16255.34Berberine bridge enzyme-like 19 OS=Arabidopsis thaliana OX=3702 GN=At4g20830 PE=... [more]
Q9SA995.9e-16154.15Berberine bridge enzyme-like 2 OS=Arabidopsis thaliana OX=3702 GN=At1g11770 PE=2... [more]
F4HV094.7e-15852.84Berberine bridge enzyme-like 14 OS=Arabidopsis thaliana OX=3702 GN=At1g34575 PE=... [more]
Match NameE-valueIdentityDescription
XP_004135723.10.0100.00berberine bridge enzyme-like 8 [Cucumis sativus] >KAE8653304.1 hypothetical prot... [more]
XP_008445642.10.095.86PREDICTED: reticuline oxidase-like protein [Cucumis melo] >KAA0047124.1 reticuli... [more]
XP_038895093.10.094.17berberine bridge enzyme-like 8 [Benincasa hispida][more]
XP_022994779.10.088.91berberine bridge enzyme-like 8 [Cucurbita maxima][more]
XP_023542409.10.089.25berberine bridge enzyme-like 8 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A5A7TVZ20.095.86Reticuline oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S3BE210.095.86reticuline oxidase-like protein OS=Cucumis melo OX=3656 GN=LOC103488597 PE=3 SV=... [more]
A0A6J1K3U30.088.91berberine bridge enzyme-like 8 OS=Cucurbita maxima OX=3661 GN=LOC111490407 PE=3 ... [more]
A0A6J1GSQ10.088.35berberine bridge enzyme-like 8 OS=Cucurbita moschata OX=3662 GN=LOC111456785 PE=... [more]
A0A6J1DGF30.084.37berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020214 PE... [more]
Match NameE-valueIdentityDescription
AT1G30700.11.8e-19764.45FAD-binding Berberine family protein [more]
AT4G20840.16.3e-16657.03FAD-binding Berberine family protein [more]
AT4G20830.13.8e-16355.34FAD-binding Berberine family protein [more]
AT4G20830.23.8e-16355.34FAD-binding Berberine family protein [more]
AT1G11770.14.2e-16254.15FAD-binding Berberine family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 25..132
e-value: 1.4E-40
score: 139.5
IPR012951Berberine/berberine-likePFAMPF08031BBEcoord: 470..527
e-value: 4.8E-21
score: 74.6
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 80..214
e-value: 3.1E-24
score: 85.3
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 136..527
e-value: 4.2E-165
score: 551.7
NoneNo IPR availableGENE3D3.40.462.20coord: 251..466
e-value: 4.2E-165
score: 551.7
NoneNo IPR availablePANTHERPTHR32448:SF25BERBERINE BRIDGE ENZYME-LIKE 8coord: 18..528
NoneNo IPR availablePANTHERPTHR32448OS08G0158400 PROTEINcoord: 18..528
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 73..248
score: 15.434865
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 21..249

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy1G024210.2CsGy1G024210.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0071949 FAD binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding