CsGy1G023855 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy1G023855
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionStructure-specific endonuclease subunit SLX1 homolog
LocationGy14Chr1: 22549615 .. 22551766 (+)
RNA-Seq ExpressionCsGy1G023855
SyntenyCsGy1G023855
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGGAAGAGGAAAGAGAAACCGGAAATCTGTAAAACCACGGATGAGGAGAAGGAAGACGATGAAGAAGAAGAGAGAGGAAACGAAGTGAATGGCTTCTTTTCATGCTATCTCTTGGCCTCTGCTTGCCCTCGTTTCAAAGGCCATACCTATATCGGGTTTGAAACTTTCCCACTCCATTTTTAGCTCCTTGCTTCCCTACATCGGACCTTCTATTTGCTTTCTAAATTCCCAATTTTCGAATTTCTTTACCATCTTTGATTCGGTTCTTTTGTAAAGATTGAGTGAGGGAAAACATAAAGAAATGTTGGTGAGTCGATCTGATCTGCTGGGTATTGATTTGTTGACTGTTTATATGAAATTTTAGATTCACAGTGAACCCGAAGCGCAGAATCAGACAACACAATGGTGAGATTAGATGCGGTGCTTGGCGTACTAAGAGGAAGAGACCTTGGGAGATGGTCTTGTGCATTTATGGCTTCCCTACTAATGTTTCTGCTCTTCAGGTTACATTTCCTGACTTTCAGCTCTAAACTAGTTTGATTCTGCGTTTTTTCTACTTGATTCTCTTTTACTAGCTGAATGAATGACACTTCGATGATTATGCAAATGTTGAGATGTTAGATTAAAAGAAGAAAAAAAGCAGATTCTCTTTCAGAACTCATTTTTTTTATCATAAGAAATAGTGGCTTGCATATTAGGGATTTTATGGCTTAAAGAATATACCAAAATTTGTTGCTGCTTTAATTTTCTTCTTTAGCCGTTTACTTTCTTGTAAGTTTCCATCAGTTCTAGAATTTGATTTTCTAAACTGTGGGTACAGTTTGAATGGGCGTGGCAGCATCCTAATGAGTCACTGGCTGTAAGAAGTGCTGCTGCAACTTTCAAGTCTCTCTCTGGAGTTGCTAACAAAGTTAAACTTGCATACACAATGCTCACACTTCCTGCTTGGCGTGGGTATGACATTTGAAAAAATTTATACAATCTTGTCGAATTTGTCTGTTAAATATGCGAGAAGCGTGAAGAGAAACCTAAAAATAATGAAAATATCCATCAACATGTATTGAAAGGTTTGACATTGATATTAAATATTATTGAACATCTCCAATATCTTCTATAAATGCTTGTAAACATCAAATCTTCAAATCGATGTGGACATGAGTATTTTAATCCTTGGATAATCCTCCATATCAGCAAGAAAAGGGAAAGATTTATAGTATCTCAATAAAGACAACAATCTTATAACTGTCTTGTTTTTGGGGCAAAACCAAAGTTTGAGAGTGAAAAGGGCGATATCTTATTACTGTGATTTATGAAGGAGCAACAAACTGATGTAATTTCAATCAATTTTGTCTGGCAATCTCTTTATTCACTTATTTCTCCTTATCCTTGCAGTTTAAATATAACTGTAAACTACTTCTCTACAAAGTTCATGAAGAATGCGGCTGGTTGCCCAAGTTTGCCAGAGCATATGAAGGTCCAAGTTTCCCCCATCAATGAGCTTCCATGCTATTCCGAAGGAGATCAAGATATGCTTGAAAATGAAGGTGACTGGGAGTATAATCGAGAACGTGAAGAAATTTGTGGTTTTCGAGTGTATGGATCAATGAAAGAAGTTTCAAATGAAGTTCCTCAAAAGTTAATGGATTATCAGACAGGTACAGATGGAAGACCTCCTCACGTGTTGCGTGGATGTGATAAGGAGCTTGAGACCAATGAACAAGTGCCACCTTCTTCATGTACTCCGTCTTATATTGATGTGGGTATGTCTTATGACTTGTGTGCATGTGATGAAGGACTCGAGAATGATGAAAGAGAGGCAGCTTCGTGTGGTCAGTCTTGCATTGTAGCAGGTACATCTAGAACTGAAATTGTCATTGATGACGAAGAGGAAAACCAATTAGAAGGGAGTAGTATGAACTTGCAGGAGCAACCAGGTAGGGAGAATTTAACATCAGGAATAGCTTCTGAAATCAGTAAAGTTTCAAGGTGGAACAATGGCTGGGTTCCAACTGTTGAGTATGAGGTTATCGATGTGTCTACCCCGTCTCCAGACTGCCGAACAAGTTCGCATAGATTCAAGAGAAGAGTTACTTCCGGTAAGTCTGAGATGATTGACTTGACTAAATCTCCTACATTTATTCAATTGTAG

mRNA sequence

ATGCGGAAGAGGAAAGAGAAACCGGAAATCTGTAAAACCACGGATGAGGAGAAGGAAGACGATGAAGAAGAAGAGAGAGGAAACGAAGTGAATGGCTTCTTTTCATGCTATCTCTTGGCCTCTGCTTGCCCTCGTTTCAAAGGCCATACCTATATCGGATTCACAGTGAACCCGAAGCGCAGAATCAGACAACACAATGGTGAGATTAGATGCGGTGCTTGGCGTACTAAGAGGAAGAGACCTTGGGAGATGGTCTTGTGCATTTATGGCTTCCCTACTAATGTTTCTGCTCTTCAGTTTGAATGGGCGTGGCAGCATCCTAATGAGTCACTGGCTGTAAGAAGTGCTGCTGCAACTTTCAAGTCTCTCTCTGGAGTTGCTAACAAAGTTAAACTTGCATACACAATGCTCACACTTCCTGCTTGGCGTGGTTTAAATATAACTGTAAACTACTTCTCTACAAAGTTCATGAAGAATGCGGCTGGTTGCCCAAGTTTGCCAGAGCATATGAAGGTCCAAGTTTCCCCCATCAATGAGCTTCCATGCTATTCCGAAGGAGATCAAGATATGCTTGAAAATGAAGGTGACTGGGAGTATAATCGAGAACGTGAAGAAATTTGTGGTTTTCGAGTGTATGGATCAATGAAAGAAGTTTCAAATGAAGTTCCTCAAAAGTTAATGGATTATCAGACAGGTACAGATGGAAGACCTCCTCACGTGTTGCGTGGATGTGATAAGGAGCTTGAGACCAATGAACAAGTGCCACCTTCTTCATGTACTCCGTCTTATATTGATGTGGGTATGTCTTATGACTTGTGTGCATGTGATGAAGGACTCGAGAATGATGAAAGAGAGGCAGCTTCGTGTGGTCAGTCTTGCATTGTAGCAGGTACATCTAGAACTGAAATTGTCATTGATGACGAAGAGGAAAACCAATTAGAAGGGAGTAGTATGAACTTGCAGGAGCAACCAGGTAGGGAGAATTTAACATCAGGAATAGCTTCTGAAATCAGTAAAGTTTCAAGGTGGAACAATGGCTGGGTTCCAACTGTTGAGTATGAGGTTATCGATGTGTCTACCCCGTCTCCAGACTGCCGAACAAGTTCGCATAGATTCAAGAGAAGAGTTACTTCCGGTAAGTCTGAGATGATTGACTTGACTAAATCTCCTACATTTATTCAATTGTAG

Coding sequence (CDS)

ATGCGGAAGAGGAAAGAGAAACCGGAAATCTGTAAAACCACGGATGAGGAGAAGGAAGACGATGAAGAAGAAGAGAGAGGAAACGAAGTGAATGGCTTCTTTTCATGCTATCTCTTGGCCTCTGCTTGCCCTCGTTTCAAAGGCCATACCTATATCGGATTCACAGTGAACCCGAAGCGCAGAATCAGACAACACAATGGTGAGATTAGATGCGGTGCTTGGCGTACTAAGAGGAAGAGACCTTGGGAGATGGTCTTGTGCATTTATGGCTTCCCTACTAATGTTTCTGCTCTTCAGTTTGAATGGGCGTGGCAGCATCCTAATGAGTCACTGGCTGTAAGAAGTGCTGCTGCAACTTTCAAGTCTCTCTCTGGAGTTGCTAACAAAGTTAAACTTGCATACACAATGCTCACACTTCCTGCTTGGCGTGGTTTAAATATAACTGTAAACTACTTCTCTACAAAGTTCATGAAGAATGCGGCTGGTTGCCCAAGTTTGCCAGAGCATATGAAGGTCCAAGTTTCCCCCATCAATGAGCTTCCATGCTATTCCGAAGGAGATCAAGATATGCTTGAAAATGAAGGTGACTGGGAGTATAATCGAGAACGTGAAGAAATTTGTGGTTTTCGAGTGTATGGATCAATGAAAGAAGTTTCAAATGAAGTTCCTCAAAAGTTAATGGATTATCAGACAGGTACAGATGGAAGACCTCCTCACGTGTTGCGTGGATGTGATAAGGAGCTTGAGACCAATGAACAAGTGCCACCTTCTTCATGTACTCCGTCTTATATTGATGTGGGTATGTCTTATGACTTGTGTGCATGTGATGAAGGACTCGAGAATGATGAAAGAGAGGCAGCTTCGTGTGGTCAGTCTTGCATTGTAGCAGGTACATCTAGAACTGAAATTGTCATTGATGACGAAGAGGAAAACCAATTAGAAGGGAGTAGTATGAACTTGCAGGAGCAACCAGGTAGGGAGAATTTAACATCAGGAATAGCTTCTGAAATCAGTAAAGTTTCAAGGTGGAACAATGGCTGGGTTCCAACTGTTGAGTATGAGGTTATCGATGTGTCTACCCCGTCTCCAGACTGCCGAACAAGTTCGCATAGATTCAAGAGAAGAGTTACTTCCGGTAAGTCTGAGATGATTGACTTGACTAAATCTCCTACATTTATTCAATTGTAG

Protein sequence

MRKRKEKPEICKTTDEEKEDDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPINELPCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRPPHVLRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAGTSRTEIVIDDEEENQLEGSSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL*
Homology
BLAST of CsGy1G023855 vs. ExPASy Swiss-Prot
Match: Q8BX32 (Structure-specific endonuclease subunit SLX1 OS=Mus musculus OX=10090 GN=Slx1b PE=2 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 1.1e-29
Identity = 67/164 (40.85%), Postives = 92/164 (56.10%), Query Frame = 0

Query: 33  FFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHN-GEIRCGAWRTKRKRPWEMVLCIYGF 92
           FF  YLL    PR +G  Y+GFTVNP RR+RQHN G  + GAWRT  + PW+MVL I+GF
Sbjct: 10  FFGVYLLYCQNPRHRGRVYVGFTVNPARRVRQHNAGRKKGGAWRTSGRGPWDMVLIIHGF 69

Query: 93  PTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNY 152
           P+ V+AL+FEWAWQHP  S  +       +S +  A  +++   ML +P W  L +T+ +
Sbjct: 70  PSAVAALRFEWAWQHPQASRRLTHVGPRLRSEAAFAFHLRVLAHMLRVPPWVRLPLTLRW 129

Query: 153 FSTKFMKNAAGCPSLPEHMKVQVSP------INELPCYSEGDQD 190
               F      CP+ P HM +   P      + + P  SE D +
Sbjct: 130 LRPDFRHEL--CPAPPAHMPIAFGPPPPQPLVPKRPAVSEADSE 171

BLAST of CsGy1G023855 vs. ExPASy Swiss-Prot
Match: Q5PQP5 (Structure-specific endonuclease subunit SLX1 OS=Rattus norvegicus OX=10116 GN=Slx1b PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 3.1e-29
Identity = 66/164 (40.24%), Postives = 91/164 (55.49%), Query Frame = 0

Query: 33  FFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHN-GEIRCGAWRTKRKRPWEMVLCIYGF 92
           FF  YLL    PR +G  Y+GFTVNP RR+RQHN G  + GAWRT  + PW+MVL ++GF
Sbjct: 10  FFGVYLLYCQNPRHRGRVYVGFTVNPARRVRQHNAGRKKGGAWRTSGRGPWDMVLILHGF 69

Query: 93  PTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNY 152
           P+ V+AL+FEWAWQHP  S  +       +S +     +++   ML +P W  L +TV +
Sbjct: 70  PSAVAALRFEWAWQHPQASRRLTHVGPRLRSEASFTFHLRVLAHMLRVPPWVRLPLTVRW 129

Query: 153 FSTKFMKNAAGCPSLPEHMKVQVSP------INELPCYSEGDQD 190
               F      CP+ P HM +   P      + + P  SE D +
Sbjct: 130 LRPDFRHEL--CPAPPPHMPIAFGPPPPQPLVPKRPAASEADSE 171

BLAST of CsGy1G023855 vs. ExPASy Swiss-Prot
Match: Q32PI0 (Structure-specific endonuclease subunit SLX1 OS=Bos taurus OX=9913 GN=SLX1A PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 5.8e-28
Identity = 61/145 (42.07%), Postives = 84/145 (57.93%), Query Frame = 0

Query: 33  FFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHN-GEIRCGAWRTKRKRPWEMVLCIYGF 92
           FF  YLL    PR +G  Y+GFTVNP RR++QHN G  + GAWRT  + PWEMVL ++GF
Sbjct: 6   FFGVYLLYCLNPRHRGRVYVGFTVNPARRVQQHNGGRKKGGAWRTSGRGPWEMVLIVHGF 65

Query: 93  PTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNY 152
           P+ V+AL+FEWAWQHP  S  +       +  +  A  +++   ML  P W  L +T+ +
Sbjct: 66  PSAVAALRFEWAWQHPQASRRLTHVGRRLRGEATFAFHLRVLAHMLRAPPWVRLPLTLRW 125

Query: 153 FSTKFMKNAAGCPSLPEHMKVQVSP 177
               F ++   CP  P HM +   P
Sbjct: 126 LRADFRQDL--CPPPPPHMPLAFGP 148

BLAST of CsGy1G023855 vs. ExPASy Swiss-Prot
Match: Q0IH86 (Structure-specific endonuclease subunit slx1 OS=Xenopus laevis OX=8355 GN=slx1a PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 2.9e-27
Identity = 59/154 (38.31%), Postives = 88/154 (57.14%), Query Frame = 0

Query: 29  EVNGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHN-GEIRCGAWRTKRKRPWEMVLC 88
           EV GF+  YLL    P++KG  YIGFTVNP+RRI+QHN G+ + GAW+T  + PW+MVL 
Sbjct: 4   EVEGFYGVYLLFCTNPKYKGRIYIGFTVNPERRIQQHNGGKHKGGAWKTSGRGPWDMVLI 63

Query: 89  IYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWRGLNI 148
           ++GFP +++AL+FEWAWQHP+ S  +       K  S     + +   ML +  W  L +
Sbjct: 64  VHGFPNDIAALRFEWAWQHPHVSRRLTHVPRKTKKQSSFDFHLLVLCHMLRVAPWNRLPL 123

Query: 149 TVNYFSTKFMKNAAGCPSLPEHMKVQVSPINELP 182
           T+ +   ++ +        P HM +    +   P
Sbjct: 124 TLRWLRQEYRRELPLLLQPPLHMPLAFGQVRARP 157

BLAST of CsGy1G023855 vs. ExPASy Swiss-Prot
Match: A7STV9 (Structure-specific endonuclease subunit SLX1 homolog OS=Nematostella vectensis OX=45351 GN=v1g174298 PE=3 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.9e-26
Identity = 61/149 (40.94%), Postives = 87/149 (58.39%), Query Frame = 0

Query: 31  NGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEI-RCGAWRTKRKRPWEMVLCIY 90
           +GF   YLL    P+FKGHTYIG+TVNP RRI+QHNG + + GA++T RK+PW M+L ++
Sbjct: 8   SGFHGVYLLYCVNPKFKGHTYIGYTVNPNRRIKQHNGGVDKGGAYKTSRKKPWNMILIVH 67

Query: 91  GFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLS-GVANKVKLAYTMLTLPAWRGLNIT 150
           GFP ++ ALQFEWAWQ P  S  ++SAA   K     V    ++   +L +  W  L + 
Sbjct: 68  GFPNDIIALQFEWAWQKPTVSRRLKSAATKKKPRERPVQYCFRILSELLRVGPWNRLPLH 127

Query: 151 VNYFSTKFMKNAAGCPSLPEHMKVQVSPI 178
           + +   ++          P HM V   P+
Sbjct: 128 IRWLMREYEMEFDPRCLPPFHMTVVYGPL 156

BLAST of CsGy1G023855 vs. NCBI nr
Match: XP_004153269.1 (structure-specific endonuclease subunit SLX1 homolog [Cucumis sativus])

HSP 1 Score: 813 bits (2101), Expect = 1.32e-297
Identity = 395/395 (100.00%), Postives = 395/395 (100.00%), Query Frame = 0

Query: 1   MRKRKEKPEICKTTDEEKEDDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVNPKR 60
           MRKRKEKPEICKTTDEEKEDDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVNPKR
Sbjct: 1   MRKRKEKPEICKTTDEEKEDDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVNPKR 60

Query: 61  RIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATF 120
           RIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATF
Sbjct: 61  RIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATF 120

Query: 121 KSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPINEL 180
           KSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPINEL
Sbjct: 121 KSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPINEL 180

Query: 181 PCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRPPHV 240
           PCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRPPHV
Sbjct: 181 PCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRPPHV 240

Query: 241 LRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAGTSR 300
           LRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAGTSR
Sbjct: 241 LRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAGTSR 300

Query: 301 TEIVIDDEEENQLEGSSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVST 360
           TEIVIDDEEENQLEGSSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVST
Sbjct: 301 TEIVIDDEEENQLEGSSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVST 360

Query: 361 PSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 395
           PSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL
Sbjct: 361 PSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 395

BLAST of CsGy1G023855 vs. NCBI nr
Match: XP_008457313.1 (PREDICTED: uncharacterized protein LOC103497035 [Cucumis melo])

HSP 1 Score: 752 bits (1941), Expect = 4.05e-273
Identity = 373/401 (93.02%), Postives = 381/401 (95.01%), Query Frame = 0

Query: 1   MRKRKEKPEICKTT-----DEEKEDD-EEEERGNEVNGFFSCYLLASACPRFKGHTYIGF 60
           MRKRKEKPEICKT      DEEKEDD EEEERGN VNGFFSCYLLASACPRFKGHTYIGF
Sbjct: 1   MRKRKEKPEICKTMAAVVQDEEKEDDDEEEERGNGVNGFFSCYLLASACPRFKGHTYIGF 60

Query: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR 120
           TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR
Sbjct: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR 120

Query: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMKVQV 180
           SAAATFKSLSGVANKVKLAYTMLTLPAW GLNITVNYFSTKFMKNAAGCPSLPEHMKVQV
Sbjct: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWCGLNITVNYFSTKFMKNAAGCPSLPEHMKVQV 180

Query: 181 SPINELPCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTD 240
           SPI+ELPCYSEGDQ +LENE DWEY+RE EEIC F+VYGSMKEVSNEVPQKLMDYQTGTD
Sbjct: 181 SPIDELPCYSEGDQGVLENEDDWEYDRESEEICSFQVYGSMKEVSNEVPQKLMDYQTGTD 240

Query: 241 GRPPHVLRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCI 300
           GRPP VLRGCDKELETNEQVPPSSCTPSY+DVGMSYDLCACDEGLENDEREAASCGQSC 
Sbjct: 241 GRPPPVLRGCDKELETNEQVPPSSCTPSYVDVGMSYDLCACDEGLENDEREAASCGQSCT 300

Query: 301 VAGTSRTEIVIDDEEENQLEGSSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTVEYE 360
           VAGTSRTEI+IDD EENQ EGS MNLQEQPGR+NLTSGIASEISKVSR +NG VPTVEYE
Sbjct: 301 VAGTSRTEIIIDDGEENQFEGSGMNLQEQPGRKNLTSGIASEISKVSRCSNGLVPTVEYE 360

Query: 361 VIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 395
           VIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL
Sbjct: 361 VIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 401

BLAST of CsGy1G023855 vs. NCBI nr
Match: KAA0060004.1 (Structure-specific endonuclease subunit SLX1 [Cucumis melo var. makuwa] >TYJ97262.1 Structure-specific endonuclease subunit SLX1 [Cucumis melo var. makuwa])

HSP 1 Score: 735 bits (1897), Expect = 1.26e-266
Identity = 360/382 (94.24%), Postives = 368/382 (96.34%), Query Frame = 0

Query: 15  DEEKEDD-EEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEIRCGA 74
           DEEKEDD EEEERGN VNGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEIRCGA
Sbjct: 7   DEEKEDDDEEEERGNGVNGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEIRCGA 66

Query: 75  WRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLA 134
           WRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLA
Sbjct: 67  WRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLA 126

Query: 135 YTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPINELPCYSEGDQDMLEN 194
           YTMLTLPAW GLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPI+ELPCYSEGDQ +LEN
Sbjct: 127 YTMLTLPAWCGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPIDELPCYSEGDQGVLEN 186

Query: 195 EGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRPPHVLRGCDKELETNEQ 254
           E DWEY+RE EEIC F+VYGSMKEVSNEVPQKLMDYQTGTDGRPP VLRGCDKELETNEQ
Sbjct: 187 EDDWEYDRESEEICSFQVYGSMKEVSNEVPQKLMDYQTGTDGRPPPVLRGCDKELETNEQ 246

Query: 255 VPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAGTSRTEIVIDDEEENQL 314
           VPPSSCTPSY+DVGMSYDLCACDEGLENDEREAASCGQSC VAGTSRTEI+IDD EENQ 
Sbjct: 247 VPPSSCTPSYVDVGMSYDLCACDEGLENDEREAASCGQSCTVAGTSRTEIIIDDGEENQF 306

Query: 315 EGSSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVSTPSPDCRTSSHRFK 374
           EGS MNLQEQPGR+NLTSGIASEISKVSR +NG VPTVEYEVIDVSTPSPDCRTSSHRFK
Sbjct: 307 EGSGMNLQEQPGRKNLTSGIASEISKVSRCSNGLVPTVEYEVIDVSTPSPDCRTSSHRFK 366

Query: 375 RRVTSGKSEMIDLTKSPTFIQL 395
           RRVTSGKSEMIDLTKSPTFIQL
Sbjct: 367 RRVTSGKSEMIDLTKSPTFIQL 388

BLAST of CsGy1G023855 vs. NCBI nr
Match: KGN66284.2 (hypothetical protein Csa_023148 [Cucumis sativus])

HSP 1 Score: 705 bits (1820), Expect = 2.57e-255
Identity = 341/344 (99.13%), Postives = 342/344 (99.42%), Query Frame = 0

Query: 52  IGFTVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESL 111
           I  +VNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESL
Sbjct: 19  IPISVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESL 78

Query: 112 AVRSAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMK 171
           AVRSAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMK
Sbjct: 79  AVRSAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMK 138

Query: 172 VQVSPINELPCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQT 231
           VQVSPINELPCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQT
Sbjct: 139 VQVSPINELPCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQT 198

Query: 232 GTDGRPPHVLRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQ 291
           GTDGRPPHVLRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQ
Sbjct: 199 GTDGRPPHVLRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQ 258

Query: 292 SCIVAGTSRTEIVIDDEEENQLEGSSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTV 351
           SCIVAGTSRTEIVIDDEEENQLEGSSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTV
Sbjct: 259 SCIVAGTSRTEIVIDDEEENQLEGSSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTV 318

Query: 352 EYEVIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 395
           EYEVIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL
Sbjct: 319 EYEVIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 362

BLAST of CsGy1G023855 vs. NCBI nr
Match: XP_038894793.1 (structure-specific endonuclease subunit SLX1 [Benincasa hispida])

HSP 1 Score: 661 bits (1705), Expect = 3.39e-237
Identity = 334/401 (83.29%), Postives = 355/401 (88.53%), Query Frame = 0

Query: 1   MRKRKEKPEICKT---TDEEKEDDE---EEERGNEVNGFFSCYLLASACPRFKGHTYIGF 60
           MRKRKE+PEI KT    +EEKEDDE   EEE  NEVNGFFSCYLLASACPR+KGHTYIGF
Sbjct: 1   MRKRKERPEISKTMAVAEEEKEDDEDEEEEEGSNEVNGFFSCYLLASACPRYKGHTYIGF 60

Query: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR 120
           TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR
Sbjct: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR 120

Query: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMKVQV 180
           SAAATFKSLSGVANKVKLAYTMLTLPAW  LNITVNYFSTK+MKNAAGCPSLP+HMKVQV
Sbjct: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWCSLNITVNYFSTKYMKNAAGCPSLPKHMKVQV 180

Query: 181 SPINELPCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTD 240
           SPINELPCYSEGDQ +LENE DWEYNRE EEIC FRVYGSMKEVSNEVPQKLMD+Q  TD
Sbjct: 181 SPINELPCYSEGDQCVLENEDDWEYNRECEEICSFRVYGSMKEVSNEVPQKLMDHQASTD 240

Query: 241 GRPPHVLRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCI 300
           GRP   L GCDKE E NEQVPPSSC PSYID GMSYDL  CDE LE DERE ASC  SCI
Sbjct: 241 GRP-RELHGCDKEFENNEQVPPSSCNPSYIDAGMSYDLHGCDEELEEDEREPASCAPSCI 300

Query: 301 VAGTSRTEIVIDDEEENQLEGSSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTVEYE 360
           V   SRTEI+IDD +E+Q+EG+ MNL++Q  R+NL+SGIASEISKVSR N   +P VEYE
Sbjct: 301 VTDISRTEIIIDDGDEDQIEGTGMNLEQQLCRKNLSSGIASEISKVSRCNIE-LPPVEYE 360

Query: 361 VIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 395
           VIDVSTPSPDCRT+SHR KR+VTSG+SE+IDLTKSPTFIQL
Sbjct: 361 VIDVSTPSPDCRTNSHRVKRKVTSGRSEIIDLTKSPTFIQL 399

BLAST of CsGy1G023855 vs. ExPASy TrEMBL
Match: A0A0A0M0Y6 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis sativus OX=3659 GN=Csa_1G533630 PE=3 SV=1)

HSP 1 Score: 813 bits (2101), Expect = 6.39e-298
Identity = 395/395 (100.00%), Postives = 395/395 (100.00%), Query Frame = 0

Query: 1   MRKRKEKPEICKTTDEEKEDDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVNPKR 60
           MRKRKEKPEICKTTDEEKEDDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVNPKR
Sbjct: 1   MRKRKEKPEICKTTDEEKEDDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVNPKR 60

Query: 61  RIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATF 120
           RIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATF
Sbjct: 61  RIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATF 120

Query: 121 KSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPINEL 180
           KSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPINEL
Sbjct: 121 KSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPINEL 180

Query: 181 PCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRPPHV 240
           PCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRPPHV
Sbjct: 181 PCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRPPHV 240

Query: 241 LRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAGTSR 300
           LRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAGTSR
Sbjct: 241 LRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAGTSR 300

Query: 301 TEIVIDDEEENQLEGSSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVST 360
           TEIVIDDEEENQLEGSSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVST
Sbjct: 301 TEIVIDDEEENQLEGSSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVST 360

Query: 361 PSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 395
           PSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL
Sbjct: 361 PSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 395

BLAST of CsGy1G023855 vs. ExPASy TrEMBL
Match: A0A1S3C574 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis melo OX=3656 GN=LOC103497035 PE=3 SV=1)

HSP 1 Score: 752 bits (1941), Expect = 1.96e-273
Identity = 373/401 (93.02%), Postives = 381/401 (95.01%), Query Frame = 0

Query: 1   MRKRKEKPEICKTT-----DEEKEDD-EEEERGNEVNGFFSCYLLASACPRFKGHTYIGF 60
           MRKRKEKPEICKT      DEEKEDD EEEERGN VNGFFSCYLLASACPRFKGHTYIGF
Sbjct: 1   MRKRKEKPEICKTMAAVVQDEEKEDDDEEEERGNGVNGFFSCYLLASACPRFKGHTYIGF 60

Query: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR 120
           TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR
Sbjct: 61  TVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVR 120

Query: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMKVQV 180
           SAAATFKSLSGVANKVKLAYTMLTLPAW GLNITVNYFSTKFMKNAAGCPSLPEHMKVQV
Sbjct: 121 SAAATFKSLSGVANKVKLAYTMLTLPAWCGLNITVNYFSTKFMKNAAGCPSLPEHMKVQV 180

Query: 181 SPINELPCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTD 240
           SPI+ELPCYSEGDQ +LENE DWEY+RE EEIC F+VYGSMKEVSNEVPQKLMDYQTGTD
Sbjct: 181 SPIDELPCYSEGDQGVLENEDDWEYDRESEEICSFQVYGSMKEVSNEVPQKLMDYQTGTD 240

Query: 241 GRPPHVLRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCI 300
           GRPP VLRGCDKELETNEQVPPSSCTPSY+DVGMSYDLCACDEGLENDEREAASCGQSC 
Sbjct: 241 GRPPPVLRGCDKELETNEQVPPSSCTPSYVDVGMSYDLCACDEGLENDEREAASCGQSCT 300

Query: 301 VAGTSRTEIVIDDEEENQLEGSSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTVEYE 360
           VAGTSRTEI+IDD EENQ EGS MNLQEQPGR+NLTSGIASEISKVSR +NG VPTVEYE
Sbjct: 301 VAGTSRTEIIIDDGEENQFEGSGMNLQEQPGRKNLTSGIASEISKVSRCSNGLVPTVEYE 360

Query: 361 VIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 395
           VIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL
Sbjct: 361 VIDVSTPSPDCRTSSHRFKRRVTSGKSEMIDLTKSPTFIQL 401

BLAST of CsGy1G023855 vs. ExPASy TrEMBL
Match: A0A5D3BBR0 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00560 PE=3 SV=1)

HSP 1 Score: 735 bits (1897), Expect = 6.10e-267
Identity = 360/382 (94.24%), Postives = 368/382 (96.34%), Query Frame = 0

Query: 15  DEEKEDD-EEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEIRCGA 74
           DEEKEDD EEEERGN VNGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEIRCGA
Sbjct: 7   DEEKEDDDEEEERGNGVNGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEIRCGA 66

Query: 75  WRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLA 134
           WRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLA
Sbjct: 67  WRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLA 126

Query: 135 YTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPINELPCYSEGDQDMLEN 194
           YTMLTLPAW GLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPI+ELPCYSEGDQ +LEN
Sbjct: 127 YTMLTLPAWCGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPIDELPCYSEGDQGVLEN 186

Query: 195 EGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRPPHVLRGCDKELETNEQ 254
           E DWEY+RE EEIC F+VYGSMKEVSNEVPQKLMDYQTGTDGRPP VLRGCDKELETNEQ
Sbjct: 187 EDDWEYDRESEEICSFQVYGSMKEVSNEVPQKLMDYQTGTDGRPPPVLRGCDKELETNEQ 246

Query: 255 VPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAGTSRTEIVIDDEEENQL 314
           VPPSSCTPSY+DVGMSYDLCACDEGLENDEREAASCGQSC VAGTSRTEI+IDD EENQ 
Sbjct: 247 VPPSSCTPSYVDVGMSYDLCACDEGLENDEREAASCGQSCTVAGTSRTEIIIDDGEENQF 306

Query: 315 EGSSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVSTPSPDCRTSSHRFK 374
           EGS MNLQEQPGR+NLTSGIASEISKVSR +NG VPTVEYEVIDVSTPSPDCRTSSHRFK
Sbjct: 307 EGSGMNLQEQPGRKNLTSGIASEISKVSRCSNGLVPTVEYEVIDVSTPSPDCRTSSHRFK 366

Query: 375 RRVTSGKSEMIDLTKSPTFIQL 395
           RRVTSGKSEMIDLTKSPTFIQL
Sbjct: 367 RRVTSGKSEMIDLTKSPTFIQL 388

BLAST of CsGy1G023855 vs. ExPASy TrEMBL
Match: A0A6J1HZQ2 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111469571 PE=3 SV=1)

HSP 1 Score: 580 bits (1496), Expect = 4.99e-206
Identity = 293/379 (77.31%), Postives = 322/379 (84.96%), Query Frame = 0

Query: 1   MRKRKEKPEICKT---TDEEKEDDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVN 60
           MR+RKEKPEI KT    +E+KEDDE+EERGNE NGFFSCYLLASACPR+KGHTYIGFTVN
Sbjct: 1   MRRRKEKPEIPKTMAVAEEKKEDDEDEERGNEGNGFFSCYLLASACPRYKGHTYIGFTVN 60

Query: 61  PKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAA 120
           PKRRIRQHNGEIRCGAWRTKRKRPWEMV CIYGFPTNVSALQFEWAWQHPNESLAVRSAA
Sbjct: 61  PKRRIRQHNGEIRCGAWRTKRKRPWEMVFCIYGFPTNVSALQFEWAWQHPNESLAVRSAA 120

Query: 121 ATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPI 180
           ATFKSLSG+ANK+KLAYTMLTLPAW  LNITVNYFSTK+M NAAGCPSLP HM+VQ+SPI
Sbjct: 121 ATFKSLSGIANKIKLAYTMLTLPAWCSLNITVNYFSTKYMNNAAGCPSLPGHMRVQISPI 180

Query: 181 NELPCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRP 240
           +ELPCYSEGD+ +LENE DWEYN+E EE+C  +V+G M EV NE+PQ LMDY TGTDGRP
Sbjct: 181 DELPCYSEGDRCVLENENDWEYNKEYEEVCSSQVHGLMTEVPNEIPQTLMDYSTGTDGRP 240

Query: 241 PHVLRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAG 300
            H LRG D+EL+ NEQVP SSCTPSYIDVG+SYDL  CDE      +E A C Q C V G
Sbjct: 241 -HELRGSDEELDDNEQVPHSSCTPSYIDVGVSYDLRGCDE------QEPALCAQPCTVEG 300

Query: 301 TSRTEIVIDDEEENQLEGSSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVID 360
              TEI+IDD EENQLEGS M LQ+QPGR+   SGIA+EISKVS      +P VEYEVID
Sbjct: 301 W--TEIIIDDGEENQLEGSGMKLQQQPGRKKNLSGIATEISKVSYG----LPPVEYEVID 360

Query: 361 VSTPSPDCRTSSHRFKRRV 376
           VSTPSP CRTSSH+FKRR+
Sbjct: 361 VSTPSPVCRTSSHKFKRRL 366

BLAST of CsGy1G023855 vs. ExPASy TrEMBL
Match: A0A6J1I681 (Structure-specific endonuclease subunit SLX1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111469571 PE=3 SV=1)

HSP 1 Score: 580 bits (1496), Expect = 9.28e-206
Identity = 293/379 (77.31%), Postives = 322/379 (84.96%), Query Frame = 0

Query: 1   MRKRKEKPEICKT---TDEEKEDDEEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVN 60
           MR+RKEKPEI KT    +E+KEDDE+EERGNE NGFFSCYLLASACPR+KGHTYIGFTVN
Sbjct: 1   MRRRKEKPEIPKTMAVAEEKKEDDEDEERGNEGNGFFSCYLLASACPRYKGHTYIGFTVN 60

Query: 61  PKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAA 120
           PKRRIRQHNGEIRCGAWRTKRKRPWEMV CIYGFPTNVSALQFEWAWQHPNESLAVRSAA
Sbjct: 61  PKRRIRQHNGEIRCGAWRTKRKRPWEMVFCIYGFPTNVSALQFEWAWQHPNESLAVRSAA 120

Query: 121 ATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPI 180
           ATFKSLSG+ANK+KLAYTMLTLPAW  LNITVNYFSTK+M NAAGCPSLP HM+VQ+SPI
Sbjct: 121 ATFKSLSGIANKIKLAYTMLTLPAWCSLNITVNYFSTKYMNNAAGCPSLPGHMRVQISPI 180

Query: 181 NELPCYSEGDQDMLENEGDWEYNREREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGRP 240
           +ELPCYSEGD+ +LENE DWEYN+E EE+C  +V+G M EV NE+PQ LMDY TGTDGRP
Sbjct: 181 DELPCYSEGDRCVLENENDWEYNKEYEEVCSSQVHGLMTEVPNEIPQTLMDYSTGTDGRP 240

Query: 241 PHVLRGCDKELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAG 300
            H LRG D+EL+ NEQVP SSCTPSYIDVG+SYDL  CDE      +E A C Q C V G
Sbjct: 241 -HELRGSDEELDDNEQVPHSSCTPSYIDVGVSYDLRGCDE------QEPALCAQPCTVEG 300

Query: 301 TSRTEIVIDDEEENQLEGSSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVID 360
              TEI+IDD EENQLEGS M LQ+QPGR+   SGIA+EISKVS      +P VEYEVID
Sbjct: 301 W--TEIIIDDGEENQLEGSGMKLQQQPGRKKNLSGIATEISKVSYG----LPPVEYEVID 360

Query: 361 VSTPSPDCRTSSHRFKRRV 376
           VSTPSP CRTSSH+FKRR+
Sbjct: 361 VSTPSPVCRTSSHKFKRRL 366

BLAST of CsGy1G023855 vs. TAIR 10
Match: AT2G30350.2 (Excinuclease ABC, C subunit, N-terminal )

HSP 1 Score: 269.6 bits (688), Expect = 3.9e-72
Identity = 171/389 (43.96%), Postives = 212/389 (54.50%), Query Frame = 0

Query: 22  EEEERGNEVNGFFSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEIRCGAWRTKRKRP 81
           E+   G +  GFF+CYLL S  PR KG TYIGFTVNP+RRIRQHNGEI  GAWRTK+KRP
Sbjct: 16  EDGVTGKDGKGFFACYLLTSLSPRHKGQTYIGFTVNPRRRIRQHNGEITSGAWRTKKKRP 75

Query: 82  WEMVLCIYGFPTNVSALQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAYTMLTLPA 141
           WEMVLCIYGFPTNVSALQFEWAWQHP ES+AVR AAA FKS SGVA+K+KL YTML LPA
Sbjct: 76  WEMVLCIYGFPTNVSALQFEWAWQHPRESVAVREAAAAFKSFSGVASKIKLVYTMLNLPA 135

Query: 142 WRGLNITVNYFSTKFMKNAAGCPSLPEHMKVQVSPINELPCYSE-GDQDMLENEGDWEYN 201
           W  LN+TVNYFS+K+  +    PSLP HMKVQV  + +L  +++  D    E+E   E N
Sbjct: 136 WNSLNLTVNYFSSKYAHHGGKSPSLPLHMKVQVCAMEDLQYFTKVDDSSQPEDEESPEVN 195

Query: 202 REREEICGFRVYGSMKEVSNEVPQKLMDYQTGTDGR-------PPHVLRGCDK------- 261
            E ++           + S+ + Q      + +D R       P  V    D+       
Sbjct: 196 EEDDD--------DDDDDSSNLSQPGNSNTSSSDDRHFEKAKEPVTVFDEEDRLANFSGF 255

Query: 262 -ELETNEQVPPSSCTPSYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAGTSRTEIVI 321
             L+  E V       S+I VG         E + ND   + +C            EIV 
Sbjct: 256 GLLDEEETVEDE---VSHITVGSIRATEKEPETVFNDRLASFTC--------FGLVEIVE 315

Query: 322 DDEEENQLEGSSMNLQEQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVSTPSPDC 381
           D+     +  +    +E   R N  +   +E              V+ EVID+ TPSP C
Sbjct: 316 DEVSHGTIGSTEAMEKECRKRRNHITSTTTE--------------VDVEVIDLMTPSPSC 367

Query: 382 RTSSHRFKRRVTSGKSEMIDLTKSPTFIQ 395
           R  S   +RRV    SE IDLT SP FI+
Sbjct: 376 RAGSSMKRRRV----SEFIDLTMSPNFIE 367

BLAST of CsGy1G023855 vs. TAIR 10
Match: AT2G30350.1 (Excinuclease ABC, C subunit, N-terminal )

HSP 1 Score: 144.1 bits (362), Expect = 2.5e-34
Identity = 113/313 (36.10%), Postives = 149/313 (47.60%), Query Frame = 0

Query: 98  LQFEWAWQHPNESLAVRSAAATFKSLSGVANKVKLAYTMLTLPAWRGLNITVNYFSTKFM 157
           LQFEWAWQHP ES+AVR AAA FKS SGVA+K+KL YTML LPAW  LN+TVNYFS+K+ 
Sbjct: 58  LQFEWAWQHPRESVAVREAAAAFKSFSGVASKIKLVYTMLNLPAWNSLNLTVNYFSSKYA 117

Query: 158 KNAAGCPSLPEHMKVQVSPINELPCYSE-GDQDMLENEGDWEYNREREEICGFRVYGSMK 217
            +    PSLP HMKVQV  + +L  +++  D    E+E   E N E ++           
Sbjct: 118 HHGGKSPSLPLHMKVQVCAMEDLQYFTKVDDSSQPEDEESPEVNEEDDD--------DDD 177

Query: 218 EVSNEVPQKLMDYQTGTDGR-------PPHVLRGCDK--------ELETNEQVPPSSCTP 277
           + S+ + Q      + +D R       P  V    D+         L+  E V       
Sbjct: 178 DDSSNLSQPGNSNTSSSDDRHFEKAKEPVTVFDEEDRLANFSGFGLLDEEETVEDE---V 237

Query: 278 SYIDVGMSYDLCACDEGLENDEREAASCGQSCIVAGTSRTEIVIDDEEENQLEGSSMNLQ 337
           S+I VG         E + ND   + +C            EIV D+     +  +    +
Sbjct: 238 SHITVGSIRATEKEPETVFNDRLASFTC--------FGLVEIVEDEVSHGTIGSTEAMEK 297

Query: 338 EQPGRENLTSGIASEISKVSRWNNGWVPTVEYEVIDVSTPSPDCRTSSHRFKRRVTSGKS 395
           E   R N  +   +E              V+ EVID+ TPSP CR  S   +RRV    S
Sbjct: 298 ECRKRRNHITSTTTE--------------VDVEVIDLMTPSPSCRAGSSMKRRRV----S 333

BLAST of CsGy1G023855 vs. TAIR 10
Match: AT5G43210.1 (Excinuclease ABC, C subunit, N-terminal )

HSP 1 Score: 57.0 bits (136), Expect = 4.0e-08
Identity = 30/72 (41.67%), Postives = 38/72 (52.78%), Query Frame = 0

Query: 34  FSCYLLASACPRFKGHTYIGFTVNPKRRIRQHNGEIRCGAWRTKRKRPWEMVLCIYGFPT 93
           +S YL+ S     K  TY+G T +  RR++QHNGEIR GA  +   RPW     I GF  
Sbjct: 54  WSVYLILSTTEPIK--TYVGITTDFSRRLKQHNGEIRGGAKASSAGRPWLCACIITGFTC 113

Query: 94  NVSALQFEWAWQ 106
              A  FE  W+
Sbjct: 114 LSQASSFESKWK 123

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8BX321.1e-2940.85Structure-specific endonuclease subunit SLX1 OS=Mus musculus OX=10090 GN=Slx1b P... [more]
Q5PQP53.1e-2940.24Structure-specific endonuclease subunit SLX1 OS=Rattus norvegicus OX=10116 GN=Sl... [more]
Q32PI05.8e-2842.07Structure-specific endonuclease subunit SLX1 OS=Bos taurus OX=9913 GN=SLX1A PE=2... [more]
Q0IH862.9e-2738.31Structure-specific endonuclease subunit slx1 OS=Xenopus laevis OX=8355 GN=slx1a ... [more]
A7STV91.9e-2640.94Structure-specific endonuclease subunit SLX1 homolog OS=Nematostella vectensis O... [more]
Match NameE-valueIdentityDescription
XP_004153269.11.32e-297100.00structure-specific endonuclease subunit SLX1 homolog [Cucumis sativus][more]
XP_008457313.14.05e-27393.02PREDICTED: uncharacterized protein LOC103497035 [Cucumis melo][more]
KAA0060004.11.26e-26694.24Structure-specific endonuclease subunit SLX1 [Cucumis melo var. makuwa] >TYJ9726... [more]
KGN66284.22.57e-25599.13hypothetical protein Csa_023148 [Cucumis sativus][more]
XP_038894793.13.39e-23783.29structure-specific endonuclease subunit SLX1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A0A0M0Y66.39e-298100.00Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis sativus OX=3659 ... [more]
A0A1S3C5741.96e-27393.02Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis melo OX=3656 GN=... [more]
A0A5D3BBR06.10e-26794.24Structure-specific endonuclease subunit SLX1 homolog OS=Cucumis melo var. makuwa... [more]
A0A6J1HZQ24.99e-20677.31Structure-specific endonuclease subunit SLX1 homolog OS=Cucurbita maxima OX=3661... [more]
A0A6J1I6819.28e-20677.31Structure-specific endonuclease subunit SLX1 homolog OS=Cucurbita maxima OX=3661... [more]
Match NameE-valueIdentityDescription
AT2G30350.23.9e-7243.96Excinuclease ABC, C subunit, N-terminal [more]
AT2G30350.12.5e-3436.10Excinuclease ABC, C subunit, N-terminal [more]
AT5G43210.14.0e-0841.67Excinuclease ABC, C subunit, N-terminal [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035901GIY-YIG endonuclease superfamilyGENE3D3.40.1440.10coord: 32..132
e-value: 6.2E-24
score: 85.9
IPR000305GIY-YIG endonucleasePFAMPF01541GIY-YIGcoord: 37..109
e-value: 2.2E-12
score: 47.1
IPR000305GIY-YIG endonucleasePROSITEPS50164GIY_YIGcoord: 32..114
score: 17.191767
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availablePANTHERPTHR20208:SF10STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1coord: 16..390
NoneNo IPR availablePANTHERPTHR20208STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1coord: 16..390
NoneNo IPR availableCDDcd10455GIY-YIG_SLX1coord: 33..107
e-value: 7.2875E-53
score: 168.565
IPR027520Structure-specific endonuclease subunit Slx1HAMAPMF_03100Endonuc_su_Slx1coord: 29..381
score: 23.629711

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy1G023855.1CsGy1G023855.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000724 double-strand break repair via homologous recombination
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0051569 regulation of histone H3-K4 methylation
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0006281 DNA repair
cellular_component GO:0016593 Cdc73/Paf1 complex
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0033557 Slx1-Slx4 complex
molecular_function GO:0017108 5'-flap endonuclease activity
molecular_function GO:0008821 crossover junction endodeoxyribonuclease activity
molecular_function GO:0000993 RNA polymerase II complex binding