CsGy1G013160 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy1G013160
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionprotein NUCLEAR FUSION DEFECTIVE 4-like
LocationGy14Chr1: 8542640 .. 8544361 (-)
RNA-Seq ExpressionCsGy1G013160
SyntenyCsGy1G013160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGATCAGAAGCCTGGGCTTTCATCAAACAAGTGGCTGAAGGACGATGGTTTTCGGTTTGGGCAGGTATGATGATGATGATCGGTAACGGTACAACATACATTTTCGGAACATACTCCAAAGTCCTCAAGTCAGAATTTGATTACAGCCAAACCCAAGTTAACATGTTAGGATTTGCTAAAGATCTCGGTAACAATGCTGGGATTATCGCGGGTTTACTCTCCGAGTTCGTTCCAACGTGGGTTCTCTTCATGATCGGCGCATTCCAAAACTTCACTGGCTACTTCTTGATATGGCTATCCATGACACGCCGGATCAGTCAACCAGCATTTTGGCAAATGTTTCTTTGCGTTTGCTTTGGCTCTAATTCTTCTAATTATTCCAATACAGCCATTATGGTCACTAGTCTTAGAAATTTCCCGGACCGTCGTGGTATCATTTTAGGCTTACTCAAAGGGTATGTTGGTATTGGAGGTGCCATTTTAACCCAAATTTGTTTAGGTTTTTATGGCCCTGAAGATCCATCAAATATTGTTCTTCTCTTTGCTTGGTTTCCCTCAGTCCTAATCCTTCTTATTTCCAATTCAATTCGACCAATTCATATCCGTAAGCATCCCGAAGAGCTCAAAGTTTTTTACCATTTACTTTATGTTTCTATTGTTCTAGCAATCTTCATCTTGTTTTTGACCATGTCAGAAAAACAAGTTGTTTTTTCTCAGTCTGCTTATGCGAGTGGCGCGTCCGTCGTGATTGCCTTACTCTTTTTACCTCTTTTGATTGCTTGTAGAGAAGAATTCTTATTGTACAAACTCAAGAAACAAAACCATAACCTTGAACCTTCTGTCACCCTCTCCATTATTGATCAAAAGGTACCAAACAGTCACAAACCATTTTCTACATTGGAAGAAATTGCAGAGATTTCTCCGTCTTGTTTGTCGAACATATGTAACAAACCACATCGAGGCGAGGATTTCACCATCTTACAAGCTATCTTCAGTGTAGATATGGTTTTAATTTGTTTAGCTACTTTTGCTGGATGTGGGTCTTCTCTTGCAGCCATTGATAATTTAGGTCAAATTGGCGAATCACTCGGCTACCCACCACGAGCTATAGGCATCTTCGTTTCTTGGGTTTCTATATTCAATTTCTTCGGCCGAGTCGTCTCTGGTTTCATCTCTGAATTGATGATGATCAAATATAAACTACCGCGTCCTCTAATGTTTGCCTTTGCCTTTCTTCTCACTTGCATTGGCCAGCTTTGTATAGCGTATCCGTTTCCAGGTTCCCTGTATGTAGCTTCCATCGTTATCGGATTTGGATTCGGAGCACAAAATCCATTGCTGTTCGCGGTAATCTCAGAGATGTTTGGCCTAAAACATTACTCCATATTGTTCAATTGTGGGCAATTGGCAGTCCCATTAGGGTCTTACATACTAAATGTGGATATAGTTGGGAAACTGTACGACGCGGAAGCATTACGTGAAGGTAAAAAGATGACGGGCAGGGGAATAAATTGCAGTGGAGCTCATTGTTTTGGTGGATCTTTTACAATTTTGGCTGCATCCACATTGTTTGGAGCATTGGTAATGCTTGTATTGGCTTATAGGACAAGAGAATATTATAGATGGGATGTATATAAGAATTACAAAGAGGATATGTGGATTCCTCAAGCAGAGATGGAGTTTTATCGCTTAGACAACAAGAAGAACATTGATGACTAA

mRNA sequence

ATGGGATCAGAAGCCTGGGCTTTCATCAAACAAGTGGCTGAAGGACGATGGTTTTCGGTTTGGGCAGGTATGATGATGATGATCGGTAACGGTACAACATACATTTTCGGAACATACTCCAAAGTCCTCAAGTCAGAATTTGATTACAGCCAAACCCAAGTTAACATGTTAGGATTTGCTAAAGATCTCGGTAACAATGCTGGGATTATCGCGGGTTTACTCTCCGAGTTCGTTCCAACGTGGGTTCTCTTCATGATCGGCGCATTCCAAAACTTCACTGGCTACTTCTTGATATGGCTATCCATGACACGCCGGATCAGTCAACCAGCATTTTGGCAAATGTTTCTTTGCGTTTGCTTTGGCTCTAATTCTTCTAATTATTCCAATACAGCCATTATGGTCACTAGTCTTAGAAATTTCCCGGACCGTCGTGGTATCATTTTAGGCTTACTCAAAGGGTATGTTGGTATTGGAGGTGCCATTTTAACCCAAATTTGTTTAGGTTTTTATGGCCCTGAAGATCCATCAAATATTGTTCTTCTCTTTGCTTGGTTTCCCTCAGTCCTAATCCTTCTTATTTCCAATTCAATTCGACCAATTCATATCCGTAAGCATCCCGAAGAGCTCAAAGTTTTTTACCATTTACTTTATGTTTCTATTGTTCTAGCAATCTTCATCTTGTTTTTGACCATGTCAGAAAAACAAGTTGTTTTTTCTCAGTCTGCTTATGCGAGTGGCGCGTCCGTCGTGATTGCCTTACTCTTTTTACCTCTTTTGATTGCTTGTAGAGAAGAATTCTTATTGTACAAACTCAAGAAACAAAACCATAACCTTGAACCTTCTGTCACCCTCTCCATTATTGATCAAAAGGTACCAAACAGTCACAAACCATTTTCTACATTGGAAGAAATTGCAGAGATTTCTCCGTCTTGTTTGTCGAACATATGTAACAAACCACATCGAGGCGAGGATTTCACCATCTTACAAGCTATCTTCAGTGTAGATATGGTTTTAATTTGTTTAGCTACTTTTGCTGGATGTGGGTCTTCTCTTGCAGCCATTGATAATTTAGGTCAAATTGGCGAATCACTCGGCTACCCACCACGAGCTATAGGCATCTTCGTTTCTTGGGTTTCTATATTCAATTTCTTCGGCCGAGTCGTCTCTGGTTTCATCTCTGAATTGATGATGATCAAATATAAACTACCGCGTCCTCTAATGTTTGCCTTTGCCTTTCTTCTCACTTGCATTGGCCAGCTTTGTATAGCGTATCCGTTTCCAGGTTCCCTGTATGTAGCTTCCATCGTTATCGGATTTGGATTCGGAGCACAAAATCCATTGCTGTTCGCGGTAATCTCAGAGATGTTTGGCCTAAAACATTACTCCATATTGTTCAATTGTGGGCAATTGGCAGTCCCATTAGGGTCTTACATACTAAATGTGGATATAGTTGGGAAACTGTACGACGCGGAAGCATTACGTGAAGGTAAAAAGATGACGGGCAGGGGAATAAATTGCAGTGGAGCTCATTGTTTTGGTGGATCTTTTACAATTTTGGCTGCATCCACATTGTTTGGAGCATTGGTAATGCTTGTATTGGCTTATAGGACAAGAGAATATTATAGATGGGATGTATATAAGAATTACAAAGAGGATATGTGGATTCCTCAAGCAGAGATGGAGTTTTATCGCTTAGACAACAAGAAGAACATTGATGACTAA

Coding sequence (CDS)

ATGGGATCAGAAGCCTGGGCTTTCATCAAACAAGTGGCTGAAGGACGATGGTTTTCGGTTTGGGCAGGTATGATGATGATGATCGGTAACGGTACAACATACATTTTCGGAACATACTCCAAAGTCCTCAAGTCAGAATTTGATTACAGCCAAACCCAAGTTAACATGTTAGGATTTGCTAAAGATCTCGGTAACAATGCTGGGATTATCGCGGGTTTACTCTCCGAGTTCGTTCCAACGTGGGTTCTCTTCATGATCGGCGCATTCCAAAACTTCACTGGCTACTTCTTGATATGGCTATCCATGACACGCCGGATCAGTCAACCAGCATTTTGGCAAATGTTTCTTTGCGTTTGCTTTGGCTCTAATTCTTCTAATTATTCCAATACAGCCATTATGGTCACTAGTCTTAGAAATTTCCCGGACCGTCGTGGTATCATTTTAGGCTTACTCAAAGGGTATGTTGGTATTGGAGGTGCCATTTTAACCCAAATTTGTTTAGGTTTTTATGGCCCTGAAGATCCATCAAATATTGTTCTTCTCTTTGCTTGGTTTCCCTCAGTCCTAATCCTTCTTATTTCCAATTCAATTCGACCAATTCATATCCGTAAGCATCCCGAAGAGCTCAAAGTTTTTTACCATTTACTTTATGTTTCTATTGTTCTAGCAATCTTCATCTTGTTTTTGACCATGTCAGAAAAACAAGTTGTTTTTTCTCAGTCTGCTTATGCGAGTGGCGCGTCCGTCGTGATTGCCTTACTCTTTTTACCTCTTTTGATTGCTTGTAGAGAAGAATTCTTATTGTACAAACTCAAGAAACAAAACCATAACCTTGAACCTTCTGTCACCCTCTCCATTATTGATCAAAAGGTACCAAACAGTCACAAACCATTTTCTACATTGGAAGAAATTGCAGAGATTTCTCCGTCTTGTTTGTCGAACATATGTAACAAACCACATCGAGGCGAGGATTTCACCATCTTACAAGCTATCTTCAGTGTAGATATGGTTTTAATTTGTTTAGCTACTTTTGCTGGATGTGGGTCTTCTCTTGCAGCCATTGATAATTTAGGTCAAATTGGCGAATCACTCGGCTACCCACCACGAGCTATAGGCATCTTCGTTTCTTGGGTTTCTATATTCAATTTCTTCGGCCGAGTCGTCTCTGGTTTCATCTCTGAATTGATGATGATCAAATATAAACTACCGCGTCCTCTAATGTTTGCCTTTGCCTTTCTTCTCACTTGCATTGGCCAGCTTTGTATAGCGTATCCGTTTCCAGGTTCCCTGTATGTAGCTTCCATCGTTATCGGATTTGGATTCGGAGCACAAAATCCATTGCTGTTCGCGGTAATCTCAGAGATGTTTGGCCTAAAACATTACTCCATATTGTTCAATTGTGGGCAATTGGCAGTCCCATTAGGGTCTTACATACTAAATGTGGATATAGTTGGGAAACTGTACGACGCGGAAGCATTACGTGAAGGTAAAAAGATGACGGGCAGGGGAATAAATTGCAGTGGAGCTCATTGTTTTGGTGGATCTTTTACAATTTTGGCTGCATCCACATTGTTTGGAGCATTGGTAATGCTTGTATTGGCTTATAGGACAAGAGAATATTATAGATGGGATGTATATAAGAATTACAAAGAGGATATGTGGATTCCTCAAGCAGAGATGGAGTTTTATCGCTTAGACAACAAGAAGAACATTGATGACTAA

Protein sequence

MGSEAWAFIKQVAEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAKDLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFGSNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQICLGFYGPEDPSNIVLLFAWFPSVLILLISNSIRPIHIRKHPEELKVFYHLLYVSIVLAIFILFLTMSEKQVVFSQSAYASGASVVIALLFLPLLIACREEFLLYKLKKQNHNLEPSVTLSIIDQKVPNSHKPFSTLEEIAEISPSCLSNICNKPHRGEDFTILQAIFSVDMVLICLATFAGCGSSLAAIDNLGQIGESLGYPPRAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFAFAFLLTCIGQLCIAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNVDIVGKLYDAEALREGKKMTGRGINCSGAHCFGGSFTILAASTLFGALVMLVLAYRTREYYRWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD*
Homology
BLAST of CsGy1G013160 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 1.1e-30
Identity = 146/541 (26.99%), Postives = 230/541 (42.51%), Query Frame = 0

Query: 16  RWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAKDLGNNAGIIAGLLS 75
           +W  + A + +    GT + F  YS  LKS    SQ ++N L  A DLG   G  +G+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 76  EFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFGSNSSNYSNTAIMVT 135
            + P  V+    A   F GY + WL +T  I+ P +  +FLC      S  + NTA  + 
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTACFIL 162

Query: 136 SLRNFPDRRGIILGLLKGYVGIGGAILTQICLGFYGPEDPSNIVLL----------FAWF 195
            +R+FP+ R + L L   + GI  A L  +      P   SN+ LL          FA  
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAA-LYSLAFNAINPSS-SNLYLLLNSLVPLVVSFAAL 222

Query: 196 PSVLILLISNSIRPIHIRKHPEELKVFYHLLYVSIVLAIFILFLTMSEKQVVFSQSAYAS 255
             VL     ++      R+H   +   + +L V  V+  F L L+ S      + SA  +
Sbjct: 223 YPVLTKPSLDTTPDYDSRRHDSHV---FTILNVLAVITSFHLLLSSSS-----TSSARLN 282

Query: 256 GASVVIALLF---LPLLIACREEFLLYKLKKQNHNLEPSVTLSIIDQKVPNSHKPFSTLE 315
               V+ L+F    PLL+  R+ FL     + NH     V L+I + K   +     T  
Sbjct: 283 FIGAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGY 342

Query: 316 EIAEISPSCLSNICNKPHRGEDFTILQAIFSVDMVLICLATFAGCGSSLAAIDNLGQIGE 375
           E    +        N    G++ +    I  ++  L  +A F G    L   +NLGQI +
Sbjct: 343 EHMGTAKE-----GNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQ 402

Query: 376 SLGYPPRAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFAFAFLLTCIG--QL 435
           SLG     +   V+  S F+FFGR++S    + M  +++L R   FA A L T I    L
Sbjct: 403 SLGQNSTTL---VTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLL 462

Query: 436 CIAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILN 495
            ++     +L  A+ +IG   G       ++ S++FG     +  N     +P+GS +  
Sbjct: 463 AVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYG 522

Query: 496 VDIVGKLYDAEALREGKKMTGRGINCSGAHCFGGSFTILAASTLFGALVMLVLAYRTREY 542
             I   +Y+A A  +   +    I C G  C+  +F      ++ G +  L L  RT+  
Sbjct: 523 Y-IAASIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPV 562

BLAST of CsGy1G013160 vs. NCBI nr
Match: XP_004147308.1 (uncharacterized protein LOC101204150 [Cucumis sativus] >KAE8652748.1 hypothetical protein Csa_014134 [Cucumis sativus])

HSP 1 Score: 1143 bits (2956), Expect = 0.0
Identity = 573/573 (100.00%), Postives = 573/573 (100.00%), Query Frame = 0

Query: 1   MGSEAWAFIKQVAEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFA 60
           MGSEAWAFIKQVAEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFA
Sbjct: 1   MGSEAWAFIKQVAEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFA 60

Query: 61  KDLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCF 120
           KDLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCF
Sbjct: 61  KDLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCF 120

Query: 121 GSNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQICLGFYGPEDPSNIVL 180
           GSNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQICLGFYGPEDPSNIVL
Sbjct: 121 GSNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQICLGFYGPEDPSNIVL 180

Query: 181 LFAWFPSVLILLISNSIRPIHIRKHPEELKVFYHLLYVSIVLAIFILFLTMSEKQVVFSQ 240
           LFAWFPSVLILLISNSIRPIHIRKHPEELKVFYHLLYVSIVLAIFILFLTMSEKQVVFSQ
Sbjct: 181 LFAWFPSVLILLISNSIRPIHIRKHPEELKVFYHLLYVSIVLAIFILFLTMSEKQVVFSQ 240

Query: 241 SAYASGASVVIALLFLPLLIACREEFLLYKLKKQNHNLEPSVTLSIIDQKVPNSHKPFST 300
           SAYASGASVVIALLFLPLLIACREEFLLYKLKKQNHNLEPSVTLSIIDQKVPNSHKPFST
Sbjct: 241 SAYASGASVVIALLFLPLLIACREEFLLYKLKKQNHNLEPSVTLSIIDQKVPNSHKPFST 300

Query: 301 LEEIAEISPSCLSNICNKPHRGEDFTILQAIFSVDMVLICLATFAGCGSSLAAIDNLGQI 360
           LEEIAEISPSCLSNICNKPHRGEDFTILQAIFSVDMVLICLATFAGCGSSLAAIDNLGQI
Sbjct: 301 LEEIAEISPSCLSNICNKPHRGEDFTILQAIFSVDMVLICLATFAGCGSSLAAIDNLGQI 360

Query: 361 GESLGYPPRAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFAFAFLLTCIGQL 420
           GESLGYPPRAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFAFAFLLTCIGQL
Sbjct: 361 GESLGYPPRAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFAFAFLLTCIGQL 420

Query: 421 CIAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILN 480
           CIAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILN
Sbjct: 421 CIAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILN 480

Query: 481 VDIVGKLYDAEALREGKKMTGRGINCSGAHCFGGSFTILAASTLFGALVMLVLAYRTREY 540
           VDIVGKLYDAEALREGKKMTGRGINCSGAHCFGGSFTILAASTLFGALVMLVLAYRTREY
Sbjct: 481 VDIVGKLYDAEALREGKKMTGRGINCSGAHCFGGSFTILAASTLFGALVMLVLAYRTREY 540

Query: 541 YRWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 573
           YRWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD
Sbjct: 541 YRWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 573

BLAST of CsGy1G013160 vs. NCBI nr
Match: XP_008464773.1 (PREDICTED: uncharacterized protein LOC103502580 [Cucumis melo])

HSP 1 Score: 1021 bits (2641), Expect = 0.0
Identity = 505/573 (88.13%), Postives = 539/573 (94.07%), Query Frame = 0

Query: 1   MGSEAWAFIKQVAEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFA 60
           M SEAWAFIKQV +GRWFSV+AGMMMMIGNGTTYIFGTYSKVLK+EFDYSQTQVNMLGFA
Sbjct: 1   MESEAWAFIKQVVQGRWFSVYAGMMMMIGNGTTYIFGTYSKVLKTEFDYSQTQVNMLGFA 60

Query: 61  KDLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCF 120
           KDLG+N GIIAGLL+EFVPTWVLF IGAFQNFT YF IWLSMTRRI QP FWQMFL VCF
Sbjct: 61  KDLGSNVGIIAGLLAEFVPTWVLFTIGAFQNFTCYFFIWLSMTRRIPQPEFWQMFLSVCF 120

Query: 121 GSNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQICLGFYGPEDPSNIVL 180
           GSNSSNY+NTAIMV SLRNFPDRRGIILGLLKGYVGIGGAILTQI LG YGPEDPS++VL
Sbjct: 121 GSNSSNYANTAIMVISLRNFPDRRGIILGLLKGYVGIGGAILTQIYLGIYGPEDPSDLVL 180

Query: 181 LFAWFPSVLILLISNSIRPIHIRKHPEELKVFYHLLYVSIVLAIFILFLTMSEKQVVFSQ 240
           LFAWFPSVLILLIS SIRPIHIRKHPEELKVFYHLLYVSI++AIFI FL++++KQV FS 
Sbjct: 181 LFAWFPSVLILLISTSIRPIHIRKHPEELKVFYHLLYVSIIIAIFIFFLSITQKQVTFSP 240

Query: 241 SAYASGASVVIALLFLPLLIACREEFLLYKLKKQNHNLEPSVTLSIIDQKVPNSHKPFST 300
           +AYASGA VV+ALL LPLLI CREEFLLYKLKKQN N +PSV LSI+DQKV N  K FST
Sbjct: 241 AAYASGAYVVVALLCLPLLIVCREEFLLYKLKKQNLNRDPSVALSIVDQKVTNCPKSFST 300

Query: 301 LEEIAEISPSCLSNICNKPHRGEDFTILQAIFSVDMVLICLATFAGCGSSLAAIDNLGQI 360
           LEEIAEISPSC+SNICNKP RG DFT+LQAIFS+DM+LICLATF+GCGSSLAAIDNLGQI
Sbjct: 301 LEEIAEISPSCISNICNKPDRGNDFTVLQAIFSIDMLLICLATFSGCGSSLAAIDNLGQI 360

Query: 361 GESLGYPPRAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFAFAFLLTCIGQL 420
           GESLGYP RAI IFVSWVSIFNFFGRV SGFISELM+IKYKLPRPLMFAFAFLLTC+GQL
Sbjct: 361 GESLGYPARAISIFVSWVSIFNFFGRVFSGFISELMIIKYKLPRPLMFAFAFLLTCLGQL 420

Query: 421 CIAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILN 480
           CIAYPFPGS+YVAS +IGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQL VPLGSYILN
Sbjct: 421 CIAYPFPGSVYVASSIIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLVVPLGSYILN 480

Query: 481 VDIVGKLYDAEALREGKKMTGRGINCSGAHCFGGSFTILAASTLFGALVMLVLAYRTREY 540
           VDIVGKLYDAEALREG+KMTG+GI C+GAHCFGGSFTILAASTLFGALVML+LAYRTR+Y
Sbjct: 481 VDIVGKLYDAEALREGRKMTGKGITCTGAHCFGGSFTILAASTLFGALVMLLLAYRTRDY 540

Query: 541 YRWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 573
           YRWD+YKNYKEDMWIPQAEMEFYRLDN+KNIDD
Sbjct: 541 YRWDIYKNYKEDMWIPQAEMEFYRLDNRKNIDD 573

BLAST of CsGy1G013160 vs. NCBI nr
Match: XP_022964775.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 888 bits (2295), Expect = 0.0
Identity = 437/572 (76.40%), Postives = 501/572 (87.59%), Query Frame = 0

Query: 2   GSEAWAFIKQVAEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAK 61
           GSEAWAFIKQV  GRWFSV+AG++MMIGNGTTY+F TYSKV+K++FDYSQTQ+N LGFAK
Sbjct: 6   GSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 62  DLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFG 121
           DLG+N GIIAGLL+E  PTWVLF+IGAFQNFTG+FLIWLS+T RI++P FWQMF+ VCFG
Sbjct: 66  DLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFILVCFG 125

Query: 122 SNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQICLGFYGPEDPSNIVLL 181
           +NSSN++NTAIMVTS+RNFPDRRGIILGLLKGYVGIGGAI++Q+ L  YG  DPSN+VLL
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPSNLVLL 185

Query: 182 FAWFPSVLILLISNSIRPIHIRKHPEELKVFYHLLYVSIVLAIFILFLTMSEKQVVFSQS 241
           FAW PS+LIL++  SIRPI IRKHP+ELKVFY LLYVSI+LAIFILFLT+++KQVVFSQ+
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 242 AYASGASVVIALLFLPLLIACREEFLLYKLKKQNHNLEPSVTLSIIDQKVPNSHKPFSTL 301
            Y  GAS V+ LL +P+LIACREE LLYKL K+     PSV LS++ Q  P    P S  
Sbjct: 246 GYVGGASFVVCLLCVPILIACREELLLYKLNKET--AAPSVALSMLHQNPPTL--PTSAD 305

Query: 302 EEIAEISPSCLSNICNKPHRGEDFTILQAIFSVDMVLICLATFAGCGSSLAAIDNLGQIG 361
            EI EISPSC  NI NKP RGEDFTILQA+FS DM LICLATF+GCGSSLAAIDNLGQ+G
Sbjct: 306 SEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQVG 365

Query: 362 ESLGYPPRAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFAFAFLLTCIGQLC 421
           ESLGY  RAIGI VSWVSIFNFFGRV SGFISE +M KYKLPRPL FAFAFL+TC+GQL 
Sbjct: 366 ESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCVGQLF 425

Query: 422 IAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNV 481
           IAYPFPGSLY+A+I+IGFGFGAQNP+LFAVISEMFGLK YS LFNCGQLA P GSYILNV
Sbjct: 426 IAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSYILNV 485

Query: 482 DIVGKLYDAEALREGKKMTGRGINCSGAHCFGGSFTILAASTLFGALVMLVLAYRTREYY 541
           D+VGKLYD EALRE K++ G+G+NC+GAHCFGGSFT+LAA+TLFGA+VM VLAYRTRE+Y
Sbjct: 486 DVVGKLYDMEALRERKQIAGKGLNCTGAHCFGGSFTVLAAATLFGAVVMFVLAYRTREFY 545

Query: 542 RWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 573
           + DVYKN+ E++WIPQ EMEFYRLDNKKNI+D
Sbjct: 546 KRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

BLAST of CsGy1G013160 vs. NCBI nr
Match: XP_022970365.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022970399.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 888 bits (2294), Expect = 0.0
Identity = 436/572 (76.22%), Postives = 503/572 (87.94%), Query Frame = 0

Query: 2   GSEAWAFIKQVAEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAK 61
           GSEAW FIKQV  GRWFSV+AG++MMIGNGTTY+F TYSKV+K++FDYSQTQ+N LGFAK
Sbjct: 6   GSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 62  DLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFG 121
           DLG+N GIIAGLL+E  PTWVLF+IGAFQNF G+FLIWLS+T RI++P FWQMF+ VCFG
Sbjct: 66  DLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFILVCFG 125

Query: 122 SNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQICLGFYGPEDPSNIVLL 181
           +NSSN++NTAIMVTS+RNFPDRRGIILGLLKGYVGIGGAI++Q  L  YG  DPSN+VLL
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPSNLVLL 185

Query: 182 FAWFPSVLILLISNSIRPIHIRKHPEELKVFYHLLYVSIVLAIFILFLTMSEKQVVFSQS 241
           FAW PS+LIL++  SIRPI IRKHP ELKVFY LLYVSI+LAIFILFLT+++KQVVFSQ+
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 242 AYASGASVVIALLFLPLLIACREEFLLYKLKKQNHNLEPSVTLSIIDQKVPNSHKPFSTL 301
            Y  GAS V++LL +P+LIACREE LLYKL KQ     PSVT+S++ Q  P    P S  
Sbjct: 246 GYVGGASFVVSLLCVPILIACREELLLYKLNKQTAG--PSVTVSMVHQNPPTL--PASAD 305

Query: 302 EEIAEISPSCLSNICNKPHRGEDFTILQAIFSVDMVLICLATFAGCGSSLAAIDNLGQIG 361
            EI EISPSC  NI NKP RGEDFTILQA+FS DM LICLATF+GCGSSLAAIDNLGQ+G
Sbjct: 306 SEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQVG 365

Query: 362 ESLGYPPRAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFAFAFLLTCIGQLC 421
           ESLGYP RAIGI VSWVSIFNFFGRV SGFISE +M KYKLPRPL F+FAFL+TC+GQL 
Sbjct: 366 ESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQLF 425

Query: 422 IAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNV 481
           IAYPFPGS+Y+A+I+IGFGFGAQNP+LFAVISEMFGL+ YS LFNCGQLAVPLGSYILNV
Sbjct: 426 IAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILNV 485

Query: 482 DIVGKLYDAEALREGKKMTGRGINCSGAHCFGGSFTILAASTLFGALVMLVLAYRTREYY 541
           D+VGKLYD EALREGKK+ G+G+ C+GAHCFGGSFTILAA+TLFGA+VMLVLAYRTRE+Y
Sbjct: 486 DVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREFY 545

Query: 542 RWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 573
           + DVYKN+ E++WIPQ +MEFYRLD+KK+I+D
Sbjct: 546 KRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 573

BLAST of CsGy1G013160 vs. NCBI nr
Match: XP_022964780.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 886 bits (2289), Expect = 0.0
Identity = 436/572 (76.22%), Postives = 500/572 (87.41%), Query Frame = 0

Query: 2   GSEAWAFIKQVAEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAK 61
           GSEAWAFIKQV  GRWFSV+AG++MMIGNGTTY+F TYSKV+K++FDYSQTQ+N LGFAK
Sbjct: 6   GSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 62  DLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFG 121
           DLG+N GIIAGLL+E  PTWVLF+IGAFQNFTG+FLIWLS+T RI++P FWQMF+ VCFG
Sbjct: 66  DLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFILVCFG 125

Query: 122 SNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQICLGFYGPEDPSNIVLL 181
           +NSSN++NTAIMVTS+RNFPDRRGIILGLLKGYVGIGGAI++Q+ L  YG  DPSN+VLL
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPSNLVLL 185

Query: 182 FAWFPSVLILLISNSIRPIHIRKHPEELKVFYHLLYVSIVLAIFILFLTMSEKQVVFSQS 241
           FAW PS+LIL++  SIRPI IRKHP+ELKVFY LLYVSI+LAIFILFLT+++KQVVFSQ+
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 242 AYASGASVVIALLFLPLLIACREEFLLYKLKKQNHNLEPSVTLSIIDQKVPNSHKPFSTL 301
            Y  GAS V+ LL +P+LIACREE LLYKL K+     PSV LS++ Q  P    P S  
Sbjct: 246 GYVGGASFVVCLLCVPILIACREELLLYKLNKET--AAPSVALSMLHQNPPTL--PTSAD 305

Query: 302 EEIAEISPSCLSNICNKPHRGEDFTILQAIFSVDMVLICLATFAGCGSSLAAIDNLGQIG 361
            EI EISPSC  NI NKP RGEDFTILQA+FS DM LICLATF+GCGSSLAAIDNLGQ+G
Sbjct: 306 SEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQVG 365

Query: 362 ESLGYPPRAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFAFAFLLTCIGQLC 421
           ESLGY  RAIGI VSWVSIFNFFGRV SGFISE +M KYKLPRPL FAFAFL+TC+GQL 
Sbjct: 366 ESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCVGQLF 425

Query: 422 IAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNV 481
           IAYPFPGSLY+A+I+IGFGFGAQNP+LFAVISEMFGLK YS LFNCGQLA P GSYILNV
Sbjct: 426 IAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSYILNV 485

Query: 482 DIVGKLYDAEALREGKKMTGRGINCSGAHCFGGSFTILAASTLFGALVMLVLAYRTREYY 541
           D+VGKLYD EALRE K++ G+G+ C+GAHCFGGSFT+LAA+TLFGA+VM VLAYRTRE+Y
Sbjct: 486 DVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVLAYRTREFY 545

Query: 542 RWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 573
           + DVYKN+ E++WIPQ EMEFYRLDNKKNI+D
Sbjct: 546 KRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

BLAST of CsGy1G013160 vs. ExPASy TrEMBL
Match: A0A1S3CMS0 (uncharacterized protein LOC103502580 OS=Cucumis melo OX=3656 GN=LOC103502580 PE=4 SV=1)

HSP 1 Score: 1021 bits (2641), Expect = 0.0
Identity = 505/573 (88.13%), Postives = 539/573 (94.07%), Query Frame = 0

Query: 1   MGSEAWAFIKQVAEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFA 60
           M SEAWAFIKQV +GRWFSV+AGMMMMIGNGTTYIFGTYSKVLK+EFDYSQTQVNMLGFA
Sbjct: 1   MESEAWAFIKQVVQGRWFSVYAGMMMMIGNGTTYIFGTYSKVLKTEFDYSQTQVNMLGFA 60

Query: 61  KDLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCF 120
           KDLG+N GIIAGLL+EFVPTWVLF IGAFQNFT YF IWLSMTRRI QP FWQMFL VCF
Sbjct: 61  KDLGSNVGIIAGLLAEFVPTWVLFTIGAFQNFTCYFFIWLSMTRRIPQPEFWQMFLSVCF 120

Query: 121 GSNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQICLGFYGPEDPSNIVL 180
           GSNSSNY+NTAIMV SLRNFPDRRGIILGLLKGYVGIGGAILTQI LG YGPEDPS++VL
Sbjct: 121 GSNSSNYANTAIMVISLRNFPDRRGIILGLLKGYVGIGGAILTQIYLGIYGPEDPSDLVL 180

Query: 181 LFAWFPSVLILLISNSIRPIHIRKHPEELKVFYHLLYVSIVLAIFILFLTMSEKQVVFSQ 240
           LFAWFPSVLILLIS SIRPIHIRKHPEELKVFYHLLYVSI++AIFI FL++++KQV FS 
Sbjct: 181 LFAWFPSVLILLISTSIRPIHIRKHPEELKVFYHLLYVSIIIAIFIFFLSITQKQVTFSP 240

Query: 241 SAYASGASVVIALLFLPLLIACREEFLLYKLKKQNHNLEPSVTLSIIDQKVPNSHKPFST 300
           +AYASGA VV+ALL LPLLI CREEFLLYKLKKQN N +PSV LSI+DQKV N  K FST
Sbjct: 241 AAYASGAYVVVALLCLPLLIVCREEFLLYKLKKQNLNRDPSVALSIVDQKVTNCPKSFST 300

Query: 301 LEEIAEISPSCLSNICNKPHRGEDFTILQAIFSVDMVLICLATFAGCGSSLAAIDNLGQI 360
           LEEIAEISPSC+SNICNKP RG DFT+LQAIFS+DM+LICLATF+GCGSSLAAIDNLGQI
Sbjct: 301 LEEIAEISPSCISNICNKPDRGNDFTVLQAIFSIDMLLICLATFSGCGSSLAAIDNLGQI 360

Query: 361 GESLGYPPRAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFAFAFLLTCIGQL 420
           GESLGYP RAI IFVSWVSIFNFFGRV SGFISELM+IKYKLPRPLMFAFAFLLTC+GQL
Sbjct: 361 GESLGYPARAISIFVSWVSIFNFFGRVFSGFISELMIIKYKLPRPLMFAFAFLLTCLGQL 420

Query: 421 CIAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILN 480
           CIAYPFPGS+YVAS +IGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQL VPLGSYILN
Sbjct: 421 CIAYPFPGSVYVASSIIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLVVPLGSYILN 480

Query: 481 VDIVGKLYDAEALREGKKMTGRGINCSGAHCFGGSFTILAASTLFGALVMLVLAYRTREY 540
           VDIVGKLYDAEALREG+KMTG+GI C+GAHCFGGSFTILAASTLFGALVML+LAYRTR+Y
Sbjct: 481 VDIVGKLYDAEALREGRKMTGKGITCTGAHCFGGSFTILAASTLFGALVMLLLAYRTRDY 540

Query: 541 YRWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 573
           YRWD+YKNYKEDMWIPQAEMEFYRLDN+KNIDD
Sbjct: 541 YRWDIYKNYKEDMWIPQAEMEFYRLDNRKNIDD 573

BLAST of CsGy1G013160 vs. ExPASy TrEMBL
Match: A0A6J1HLS6 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464776 PE=4 SV=1)

HSP 1 Score: 888 bits (2295), Expect = 0.0
Identity = 437/572 (76.40%), Postives = 501/572 (87.59%), Query Frame = 0

Query: 2   GSEAWAFIKQVAEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAK 61
           GSEAWAFIKQV  GRWFSV+AG++MMIGNGTTY+F TYSKV+K++FDYSQTQ+N LGFAK
Sbjct: 6   GSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 62  DLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFG 121
           DLG+N GIIAGLL+E  PTWVLF+IGAFQNFTG+FLIWLS+T RI++P FWQMF+ VCFG
Sbjct: 66  DLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFILVCFG 125

Query: 122 SNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQICLGFYGPEDPSNIVLL 181
           +NSSN++NTAIMVTS+RNFPDRRGIILGLLKGYVGIGGAI++Q+ L  YG  DPSN+VLL
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPSNLVLL 185

Query: 182 FAWFPSVLILLISNSIRPIHIRKHPEELKVFYHLLYVSIVLAIFILFLTMSEKQVVFSQS 241
           FAW PS+LIL++  SIRPI IRKHP+ELKVFY LLYVSI+LAIFILFLT+++KQVVFSQ+
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 242 AYASGASVVIALLFLPLLIACREEFLLYKLKKQNHNLEPSVTLSIIDQKVPNSHKPFSTL 301
            Y  GAS V+ LL +P+LIACREE LLYKL K+     PSV LS++ Q  P    P S  
Sbjct: 246 GYVGGASFVVCLLCVPILIACREELLLYKLNKET--AAPSVALSMLHQNPPTL--PTSAD 305

Query: 302 EEIAEISPSCLSNICNKPHRGEDFTILQAIFSVDMVLICLATFAGCGSSLAAIDNLGQIG 361
            EI EISPSC  NI NKP RGEDFTILQA+FS DM LICLATF+GCGSSLAAIDNLGQ+G
Sbjct: 306 SEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQVG 365

Query: 362 ESLGYPPRAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFAFAFLLTCIGQLC 421
           ESLGY  RAIGI VSWVSIFNFFGRV SGFISE +M KYKLPRPL FAFAFL+TC+GQL 
Sbjct: 366 ESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCVGQLF 425

Query: 422 IAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNV 481
           IAYPFPGSLY+A+I+IGFGFGAQNP+LFAVISEMFGLK YS LFNCGQLA P GSYILNV
Sbjct: 426 IAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSYILNV 485

Query: 482 DIVGKLYDAEALREGKKMTGRGINCSGAHCFGGSFTILAASTLFGALVMLVLAYRTREYY 541
           D+VGKLYD EALRE K++ G+G+NC+GAHCFGGSFT+LAA+TLFGA+VM VLAYRTRE+Y
Sbjct: 486 DVVGKLYDMEALRERKQIAGKGLNCTGAHCFGGSFTVLAAATLFGAVVMFVLAYRTREFY 545

Query: 542 RWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 573
           + DVYKN+ E++WIPQ EMEFYRLDNKKNI+D
Sbjct: 546 KRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

BLAST of CsGy1G013160 vs. ExPASy TrEMBL
Match: A0A6J1I5D7 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469398 PE=4 SV=1)

HSP 1 Score: 888 bits (2294), Expect = 0.0
Identity = 436/572 (76.22%), Postives = 503/572 (87.94%), Query Frame = 0

Query: 2   GSEAWAFIKQVAEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAK 61
           GSEAW FIKQV  GRWFSV+AG++MMIGNGTTY+F TYSKV+K++FDYSQTQ+N LGFAK
Sbjct: 6   GSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 62  DLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFG 121
           DLG+N GIIAGLL+E  PTWVLF+IGAFQNF G+FLIWLS+T RI++P FWQMF+ VCFG
Sbjct: 66  DLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFILVCFG 125

Query: 122 SNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQICLGFYGPEDPSNIVLL 181
           +NSSN++NTAIMVTS+RNFPDRRGIILGLLKGYVGIGGAI++Q  L  YG  DPSN+VLL
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPSNLVLL 185

Query: 182 FAWFPSVLILLISNSIRPIHIRKHPEELKVFYHLLYVSIVLAIFILFLTMSEKQVVFSQS 241
           FAW PS+LIL++  SIRPI IRKHP ELKVFY LLYVSI+LAIFILFLT+++KQVVFSQ+
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 242 AYASGASVVIALLFLPLLIACREEFLLYKLKKQNHNLEPSVTLSIIDQKVPNSHKPFSTL 301
            Y  GAS V++LL +P+LIACREE LLYKL KQ     PSVT+S++ Q  P    P S  
Sbjct: 246 GYVGGASFVVSLLCVPILIACREELLLYKLNKQTAG--PSVTVSMVHQNPPTL--PASAD 305

Query: 302 EEIAEISPSCLSNICNKPHRGEDFTILQAIFSVDMVLICLATFAGCGSSLAAIDNLGQIG 361
            EI EISPSC  NI NKP RGEDFTILQA+FS DM LICLATF+GCGSSLAAIDNLGQ+G
Sbjct: 306 SEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQVG 365

Query: 362 ESLGYPPRAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFAFAFLLTCIGQLC 421
           ESLGYP RAIGI VSWVSIFNFFGRV SGFISE +M KYKLPRPL F+FAFL+TC+GQL 
Sbjct: 366 ESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQLF 425

Query: 422 IAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNV 481
           IAYPFPGS+Y+A+I+IGFGFGAQNP+LFAVISEMFGL+ YS LFNCGQLAVPLGSYILNV
Sbjct: 426 IAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILNV 485

Query: 482 DIVGKLYDAEALREGKKMTGRGINCSGAHCFGGSFTILAASTLFGALVMLVLAYRTREYY 541
           D+VGKLYD EALREGKK+ G+G+ C+GAHCFGGSFTILAA+TLFGA+VMLVLAYRTRE+Y
Sbjct: 486 DVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREFY 545

Query: 542 RWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 573
           + DVYKN+ E++WIPQ +MEFYRLD+KK+I+D
Sbjct: 546 KRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 573

BLAST of CsGy1G013160 vs. ExPASy TrEMBL
Match: A0A6J1HLT1 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464780 PE=4 SV=1)

HSP 1 Score: 886 bits (2289), Expect = 0.0
Identity = 436/572 (76.22%), Postives = 500/572 (87.41%), Query Frame = 0

Query: 2   GSEAWAFIKQVAEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAK 61
           GSEAWAFIKQV  GRWFSV+AG++MMIGNGTTY+F TYSKV+K++FDYSQTQ+N LGFAK
Sbjct: 6   GSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 62  DLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFG 121
           DLG+N GIIAGLL+E  PTWVLF+IGAFQNFTG+FLIWLS+T RI++P FWQMF+ VCFG
Sbjct: 66  DLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFILVCFG 125

Query: 122 SNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQICLGFYGPEDPSNIVLL 181
           +NSSN++NTAIMVTS+RNFPDRRGIILGLLKGYVGIGGAI++Q+ L  YG  DPSN+VLL
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPSNLVLL 185

Query: 182 FAWFPSVLILLISNSIRPIHIRKHPEELKVFYHLLYVSIVLAIFILFLTMSEKQVVFSQS 241
           FAW PS+LIL++  SIRPI IRKHP+ELKVFY LLYVSI+LAIFILFLT+++KQVVFSQ+
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 242 AYASGASVVIALLFLPLLIACREEFLLYKLKKQNHNLEPSVTLSIIDQKVPNSHKPFSTL 301
            Y  GAS V+ LL +P+LIACREE LLYKL K+     PSV LS++ Q  P    P S  
Sbjct: 246 GYVGGASFVVCLLCVPILIACREELLLYKLNKET--AAPSVALSMLHQNPPTL--PTSAD 305

Query: 302 EEIAEISPSCLSNICNKPHRGEDFTILQAIFSVDMVLICLATFAGCGSSLAAIDNLGQIG 361
            EI EISPSC  NI NKP RGEDFTILQA+FS DM LICLATF+GCGSSLAAIDNLGQ+G
Sbjct: 306 SEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQVG 365

Query: 362 ESLGYPPRAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFAFAFLLTCIGQLC 421
           ESLGY  RAIGI VSWVSIFNFFGRV SGFISE +M KYKLPRPL FAFAFL+TC+GQL 
Sbjct: 366 ESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCVGQLF 425

Query: 422 IAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNV 481
           IAYPFPGSLY+A+I+IGFGFGAQNP+LFAVISEMFGLK YS LFNCGQLA P GSYILNV
Sbjct: 426 IAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSYILNV 485

Query: 482 DIVGKLYDAEALREGKKMTGRGINCSGAHCFGGSFTILAASTLFGALVMLVLAYRTREYY 541
           D+VGKLYD EALRE K++ G+G+ C+GAHCFGGSFT+LAA+TLFGA+VM VLAYRTRE+Y
Sbjct: 486 DVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVLAYRTREFY 545

Query: 542 RWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 573
           + DVYKN+ E++WIPQ EMEFYRLDNKKNI+D
Sbjct: 546 KRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

BLAST of CsGy1G013160 vs. ExPASy TrEMBL
Match: A0A6J1I2B3 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469248 PE=4 SV=1)

HSP 1 Score: 885 bits (2287), Expect = 0.0
Identity = 433/572 (75.70%), Postives = 501/572 (87.59%), Query Frame = 0

Query: 2   GSEAWAFIKQVAEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAK 61
           GSEAW FIKQV  GRWFSV+AG++MMIGNGTTY+F TYSKV+K++FDYSQTQ+N LGFAK
Sbjct: 6   GSEAWGFIKQVVTGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 62  DLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFG 121
           DLG+N GIIAGLL+E  PTWVLF++GAFQNFTG+FLIWLS+T RI++P FWQMF+ VCFG
Sbjct: 66  DLGSNVGIIAGLLAEVTPTWVLFIVGAFQNFTGFFLIWLSITARIAKPKFWQMFILVCFG 125

Query: 122 SNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQICLGFYGPEDPSNIVLL 181
           +NSSN++NTAIMVTS+RNFPDRRGIILGLLKGYVGIGGAI++Q+ L  YG  DPSN+VLL
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQLYLAIYGHNDPSNLVLL 185

Query: 182 FAWFPSVLILLISNSIRPIHIRKHPEELKVFYHLLYVSIVLAIFILFLTMSEKQVVFSQS 241
           FAW PS+LIL++  SIRPI IRKHP ELKVFY LLYVSI+LAIFILFLT+++KQVVFSQ+
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 242 AYASGASVVIALLFLPLLIACREEFLLYKLKKQNHNLEPSVTLSIIDQKVPNSHKPFSTL 301
            Y  GAS V++LL +P+LIACREE LLYKL KQ     PSVT+S++ Q  P      S  
Sbjct: 246 GYVGGASFVVSLLCVPILIACREELLLYKLNKQTAG--PSVTVSMVHQNPPTLSA--SAD 305

Query: 302 EEIAEISPSCLSNICNKPHRGEDFTILQAIFSVDMVLICLATFAGCGSSLAAIDNLGQIG 361
            EI EI PSC  NI NKP RGEDFTILQA+FS DM LICLATF+GCGSSLAAIDNLGQ+G
Sbjct: 306 SEIPEILPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQVG 365

Query: 362 ESLGYPPRAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFAFAFLLTCIGQLC 421
           ESLGYP RAIGI VSWVSIFNFFGRV SGFISE +M KYKLPRPL F+FAFL+TC+GQL 
Sbjct: 366 ESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQLF 425

Query: 422 IAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNV 481
           IAYPFPGS+Y A+I+IGFGFGAQNP+LFAVISEMFGL+HYS LFNCGQL VPLGSYILNV
Sbjct: 426 IAYPFPGSVYFAAIIIGFGFGAQNPMLFAVISEMFGLRHYSTLFNCGQLTVPLGSYILNV 485

Query: 482 DIVGKLYDAEALREGKKMTGRGINCSGAHCFGGSFTILAASTLFGALVMLVLAYRTREYY 541
           D+VGKLYD EAL EGK++ G+G+ C+GAHCFGGSFTILAA+TLFGA+VMLVLAYRTRE+Y
Sbjct: 486 DVVGKLYDMEALHEGKQIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREFY 545

Query: 542 RWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 573
           + DVYKN+ E++WIPQ +MEFYRLDNKK+I+D
Sbjct: 546 KRDVYKNFNEEIWIPQTQMEFYRLDNKKSIED 573

BLAST of CsGy1G013160 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 548.9 bits (1413), Expect = 4.9e-156
Identity = 282/577 (48.87%), Postives = 393/577 (68.11%), Query Frame = 0

Query: 4   EAWAFIKQVAEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAKDL 63
           E   F+     GRWF V+A  ++M   G TY+FGTYSK +KS   Y QT +N+LGF KDL
Sbjct: 5   ETVRFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDL 64

Query: 64  GNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFGSN 123
           G N G+++GL++E  PTW +  IG+  NF GYF+IWL++T ++++P  WQM L +C G+N
Sbjct: 65  GANVGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGAN 124

Query: 124 SSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQICLGFYGPEDPSNIVLLFA 183
           S N++NT  +VT ++NFP+ RG++LGLLKGYVG+ GAI TQ+    YG  D  +++LL A
Sbjct: 125 SQNFANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYG-HDSKSLILLIA 184

Query: 184 WFPSVLILLISNSIRPIHIRKHPEELKVFYHLLYVSIVLAIFILFLTMSEKQVVFSQSAY 243
           W P+ + L+    IR   + +   EL VFY  LY+SI LA+F++ + ++EKQV FS++AY
Sbjct: 185 WLPAAVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAY 244

Query: 244 ASGASVVIALLFLPLLIACREEFLLYKLKKQNHNLEPSVTLSIIDQKVPNSHKPFSTLEE 303
           A+ A++  ALLF+PL ++ ++E  ++ + K        V +    +++       + +  
Sbjct: 245 AASATICCALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNG 304

Query: 304 IAEISPSCLSNICNKPHRGEDFTILQAIFSVDMVLICLATFAGCGSSLAAIDNLGQIGES 363
             + + SC S + + P RGED+TILQA+ S DM+++ +ATF G GSSL A+DNLGQIGES
Sbjct: 305 EEKETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGES 364

Query: 364 LGYPPRAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFAFAFLLTCIGQLCIA 423
           LGYP   +  FVS VSI+N+FGRV SGF+SE ++ KYKLPRPLM     LL+C G L IA
Sbjct: 365 LGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIA 424

Query: 424 YPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNVDI 483
           +P PGS+Y+ASI++GF FGAQ PLLFA+ISE+FGLK+YS LFNCGQLA PLGSYILNV +
Sbjct: 425 FPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRV 484

Query: 484 VGKLYDAEALREGKKMTGRG--------INCSGAHCFGGSFTILAASTLFGALVMLVLAY 543
            G LYD EAL   K++T RG        + C G+ C+   F ILAA T FGALV L LA 
Sbjct: 485 TGMLYDKEAL---KQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAI 544

Query: 544 RTREYYRWDVYKNYKEDMWIPQAEMEFYRLDNKKNID 573
           RTRE+Y+ D+YK ++E    P++E E    D++K ++
Sbjct: 545 RTREFYKGDIYKKFRES---PESESELVP-DSRKAVE 573

BLAST of CsGy1G013160 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 527.7 bits (1358), Expect = 1.2e-149
Identity = 267/560 (47.68%), Postives = 377/560 (67.32%), Query Frame = 0

Query: 11  QVAEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAKDLGNNAGII 70
           Q+  GRWF  +  +++M   G TY+FG YS  +K    Y QT +N+L F KDLG N G++
Sbjct: 16  QILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVL 75

Query: 71  AGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFGSNSSNYSNT 130
           AGLL+E  P W + +IGA  NF GYF+IWL++T RIS+P  W M L +C G+NS +++NT
Sbjct: 76  AGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANT 135

Query: 131 AIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQICLGFYGPEDPSNIVLLFAWFPSVLI 190
             +VT ++NFP+ RG++LG+LKGYVG+ GAI+TQ+   FYG ED   ++L+  W P+++ 
Sbjct: 136 GSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYG-EDTKELILMIGWLPAIVS 195

Query: 191 LLISNSIRPIHIRKHPEELKVFYHLLYVSIVLAIFILFLTMSEKQVVFSQSAYASGASVV 250
                +IR + +++   ELKVFY+ LY+S+ LA F++ + +  K   F+QS +   A+VV
Sbjct: 196 FAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVV 255

Query: 251 IALLFLPLLIACREEFLLYKLKKQNHNLEPSVTLSIIDQKVPNSHKPFSTLEEIAEI--- 310
           I LL LP+++   EE  L+K K+   N    + +     K+ +S       EE  E+   
Sbjct: 256 IVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEESKEVVEK 315

Query: 311 --SPSCLSNICNKPHRGEDFTILQAIFSVDMVLICLATFAGCGSSLAAIDNLGQIGESLG 370
             +PSC + + N P RG+D+TILQA+FSVDM+++ LAT  G G +L AIDNLGQIG SLG
Sbjct: 316 VKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNSLG 375

Query: 371 YPPRAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFAFAFLLTCIGQLCIAYP 430
           YP R++  FVS VSI+N++GRVVSG +SE+ +IKYK PRPLM     LL+C G L IA+ 
Sbjct: 376 YPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAFN 435

Query: 431 FPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNVDIVG 490
            PG LYVAS++IGF FGAQ PLLFA+ISE+FGLK+YS L+N G +A P+GSY+LNV + G
Sbjct: 436 VPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVAG 495

Query: 491 KLYDAEALREGK-----KMTGRGINCSGAHCFGGSFTILAASTLFGALVMLVLAYRTREY 550
            LYD EA ++ K     ++ G+ +NC G  CF  SF I+AA TLFG LV +VL  RT+++
Sbjct: 496 YLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTKKF 555

Query: 551 YRWDVYKNYKEDMWIPQAEM 561
           Y+ D+YK ++E     + EM
Sbjct: 556 YKSDIYKKFREKALAAEMEM 574

BLAST of CsGy1G013160 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 301.6 bits (771), Expect = 1.3e-81
Identity = 186/558 (33.33%), Postives = 301/558 (53.94%), Query Frame = 0

Query: 16  RWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAKDLGNNAGIIAGLLS 75
           RW    A M +    G  Y+FG+ S V+KS  +Y+Q +++ LG AKDLG++ G IAG LS
Sbjct: 16  RWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAGTLS 75

Query: 76  EFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFGSNSSNYSNTAIMVT 135
           E +P W   ++GA QN  GY  +WL +T R      W M + +  G+N   Y NT  +V+
Sbjct: 76  EILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGALVS 135

Query: 136 SLRNFPDRRGIILGLLKGYVGIGGAILTQICLGFYGPEDPSNIVLLFAWFPSVLILLISN 195
            ++NFP  RG ++G+LKG+ G+GGAI++QI    +   +P++++L+ A  P+V+++ +  
Sbjct: 136 GVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHS-SNPASLILMVAVTPAVVVVCLMF 195

Query: 196 SIRPI--HIRKHPEELKVFYHLLYVSIVLAIFILFLTMSEKQVVFSQSAYASGASVVIAL 255
            IRP+  H +  P +   F  +  V ++LA +++ + + +  VV S +       V+  +
Sbjct: 196 FIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVLFVI 255

Query: 256 LFLPLLIACR--------------EEFLLYKLKKQNHNLE-PSVTLSIIDQKVPNS---- 315
           L +P+L+                 EE L+ K + Q   L+ P + LS ++ + P      
Sbjct: 256 LVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEKPKDVDLL 315

Query: 316 -----HKPFSTLEE---IAEISPSCLSNICNKPHRGEDFTILQAIFSVDMVLICLATFAG 375
                HK  + L+     A    +   N    PHRGEDFT+ QA+   D  LI  +   G
Sbjct: 316 PASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIFFSLLLG 375

Query: 376 CGSSLAAIDNLGQIGESLGYPPRAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPL 435
            GS L  IDNLGQ+ +SLGY      + VS +SI+NF GR+  G+ SEL++  Y  PRP+
Sbjct: 376 SGSGLTVIDNLGQMSQSLGYD--NTHVLVSMISIWNFLGRIGGGYFSELVVRDYAYPRPV 435

Query: 436 MFAFAFLLTCIGQLCIAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFN 495
             A A L+  +G +  AY +PG++Y+ +++IG G+GA   ++ A  SE+FGLK +  L+N
Sbjct: 436 AMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLKKFGALYN 495

Query: 496 CGQLAVPLGSYILNVDIVGKLYDAEALREGKKMT---GRGINCSGAHCFGGSFTILAAST 542
              LA P GS + +  I   +YD EA R+           + C+G+ CF  +  I++   
Sbjct: 496 FLTLANPAGSLVFSGMIASSIYDREAERQAHGSVFDPDDALRCNGSICFFLTSLIMSGFC 555

BLAST of CsGy1G013160 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 293.5 bits (750), Expect = 3.7e-79
Identity = 186/570 (32.63%), Postives = 300/570 (52.63%), Query Frame = 0

Query: 9   IKQVAEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAKDLGNNAG 68
           +K     RW    A M +    G  Y+FG+ S V+KS  +Y+Q Q++ LG AKDLG++ G
Sbjct: 9   VKSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDSVG 68

Query: 69  IIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFGSNSSNYS 128
            +AG LSE +P W   ++G+ QN  GY  +WL +T R      W M + +  G+N   Y 
Sbjct: 69  FLAGTLSEILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGETYF 128

Query: 129 NTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQICLGFYGPEDPSNIVLLFAWFPSV 188
           NTA +V+ ++NFP  RG ++G+LKG+ G+GGAIL+Q+    +   D ++++ + A  PSV
Sbjct: 129 NTAALVSGVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHS-SDRASLIFMVAVAPSV 188

Query: 189 LILLISNSIRPI--HIRKHPEELKVFYHLLYVSIVLAIFILFLTMSEKQVVFSQSAYASG 248
           +++ +   IRP+  H +    +   F  +  V I+LA +++ + + E  +  S S   + 
Sbjct: 189 VVVPLMFFIRPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLVEDFIDLSHSIIIAF 248

Query: 249 ASVVIALL----FLPLLIAC----------REEFLLYKLKKQNHNLE------PSVTLSI 308
             V+ A+L    F+P+  +C           EE LL   + Q+          P +  S 
Sbjct: 249 TVVLFAILLVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQSTTPDHGPELIFSE 308

Query: 309 IDQKVPNSHKPFSTLE---EIAEISPSCLSNIC---------NKPHRGEDFTILQAIFSV 368
           ++ + P        +E    IA++    +               PHRGEDFT+ QA+   
Sbjct: 309 VEDEKPKEVDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLTQALVKA 368

Query: 369 DMVLICLATFAGCGSSLAAIDNLGQIGESLGYPPRAIGIFVSWVSIFNFFGRVVSGFISE 428
           D  LI  +   G GS L  IDNLGQ+ +SLGY      +FVS +SI+NF GR+  G+ SE
Sbjct: 369 DFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYD--NTHVFVSMISIWNFLGRIGGGYFSE 428

Query: 429 LMMIKYKLPRPLMFAFAFLLTCIGQLCIAYPFPGSLYVASIVIGFGFGAQNPLLFAVISE 488
           L++  Y  PRP+  A A L+  +G +  AY +PG++++ +++IG G+GA   ++ A  SE
Sbjct: 429 LIVRDYAYPRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYGAHWAIVPATASE 488

Query: 489 MFGLKHYSILFNCGQLAVPLGSYILNVDIVGKLYDAEALREGKKMTGRG---INCSGAHC 542
           +FGLK +  L+N   LA P GS + +  I   +YD EA R+ +         + C G+ C
Sbjct: 489 LFGLKKFGALYNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSLFNPDDVLRCRGSIC 548

BLAST of CsGy1G013160 vs. TAIR 10
Match: AT2G16660.1 (Major facilitator superfamily protein )

HSP 1 Score: 288.5 bits (737), Expect = 1.2e-77
Identity = 183/531 (34.46%), Postives = 295/531 (55.56%), Query Frame = 0

Query: 16  RWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAKDLGNNAGIIAGLLS 75
           +W      + +   +G  Y F  YS  LKS  + +Q ++N L  AKD+G   GI+AGL S
Sbjct: 14  KWLGFVTAVWVQSISGNNYTFSNYSGALKSLMNLNQLELNNLSVAKDVGKAFGILAGLAS 73

Query: 76  EFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFGSNSSNYSNTAIMVT 135
           + +PT V+ +IG F+   GY + WL ++R I    +WQM + +C G NS+ + NTA++VT
Sbjct: 74  DRLPTPVILLIGCFEGLLGYGVQWLVVSRTIQPIPYWQMCIFLCMGGNSTTWMNTAVLVT 133

Query: 136 SLRNFPDRRGIILGLLKGYVGIGGAILTQICLGFYGPEDPSNIVLLFAWFPSVLILLISN 195
            +RNF   RG + G+LKGYVG+  AI T +C   +   DP++ ++L A  P  + L    
Sbjct: 134 CIRNFRRNRGPVSGILKGYVGLSTAIFTDLCTALFS-NDPASFLVLLAVVPFAVCLTAVF 193

Query: 196 SIRPI----HIRKHPEELKVFYHLLYVSIVLAIFI-LFLTMSEKQVVFSQSAYASGASVV 255
            +R I       +  EE + F     V++V+A+++  +  +  K  VFS     + AS++
Sbjct: 194 FLREIPPASSAAEENEETRYFTIFNIVAVVVAVYLQSYDIIGIKTGVFS----VAFASIL 253

Query: 256 IALLFLPLLIACREEFLLYKLKKQNHNLEPSVTLSIIDQKVPNSHKPFSTLEEIAEISPS 315
           + LL  P+ I     F+      +  +LE  +   ++  ++  + K       +A ++  
Sbjct: 254 LFLLASPIAIPF-HSFIKSLNYGEQDDLEGRIQEPLLRSEIAAAEKEVIV---VAAVAAE 313

Query: 316 CLSNICNKPHRGEDFTILQAIFSVDMVLICLATFAGCGSSLAAIDNLGQIGESLGYPPRA 375
                  KP  GED TI++A+ +VD  ++ ++   G G+ LA ++N+GQIG +LGY    
Sbjct: 314 EEEKKRKKPVLGEDHTIMEAVLTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGY--TN 373

Query: 376 IGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFAFAFLLTCIGQLCIAYPFPGSL 435
           + IFVS  SI+ FFGR++SG +SE  + K   PRPL  A + +L  +G + +A   P SL
Sbjct: 374 VSIFVSMTSIWGFFGRILSGTLSEYFLKKAGTPRPLWNAASQILMAVGYILMALAVPNSL 433

Query: 436 YVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNVDIVGKLYDA 495
           Y+ S+V+G  +G +  +     SE+FGLK+Y +++N   L +PLGS++ +  + G LYDA
Sbjct: 434 YIGSMVVGVCYGVRLAITVPTASELFGLKYYGLIYNILVLNLPLGSFLFSGLLAGFLYDA 493

Query: 496 EALREGKKMTGRGINCSGAHCFGGSFTILAASTLFGALVMLVLAYRTREYY 542
           EA        G G  C GAHC+   F ++A +++ G  + LVLAYRT+E Y
Sbjct: 494 EA----TPTPGGGNTCVGAHCYRLIFIVMALASVIGVGLDLVLAYRTKEIY 529

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I9E11.1e-3026.99Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
XP_004147308.10.0100.00uncharacterized protein LOC101204150 [Cucumis sativus] >KAE8652748.1 hypothetica... [more]
XP_008464773.10.088.13PREDICTED: uncharacterized protein LOC103502580 [Cucumis melo][more]
XP_022964775.10.076.40protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata][more]
XP_022970365.10.076.22protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022970399.1 prote... [more]
XP_022964780.10.076.22protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A1S3CMS00.088.13uncharacterized protein LOC103502580 OS=Cucumis melo OX=3656 GN=LOC103502580 PE=... [more]
A0A6J1HLS60.076.40protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1I5D70.076.22protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469... [more]
A0A6J1HLT10.076.22protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1I2B30.075.70protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469... [more]
Match NameE-valueIdentityDescription
AT2G28120.14.9e-15648.87Major facilitator superfamily protein [more]
AT2G39210.11.2e-14947.68Major facilitator superfamily protein [more]
AT5G14120.11.3e-8133.33Major facilitator superfamily protein [more]
AT3G01930.23.7e-7932.63Major facilitator superfamily protein [more]
AT2G16660.11.2e-7734.46Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 340..538
e-value: 2.9E-10
score: 39.6
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 310..551
e-value: 5.8E-12
score: 47.4
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 16..539
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 16..261
e-value: 1.7E-74
score: 250.4
NoneNo IPR availablePANTHERPTHR21576:SF29PROTEIN NUCLEAR FUSION DEFECTIVE 4coord: 3..552
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 3..552

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy1G013160.1CsGy1G013160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity