Cp4.1LG20g09160 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG20g09160
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptiontRNA ligase 1
LocationCp4.1LG20: 6712781 .. 6713386 (+)
RNA-Seq ExpressionCp4.1LG20g09160
SyntenyCp4.1LG20g09160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGGCGCCGCATAGGATTTTCCGTGCTATAACTCTTCCTCTCCCTCGTTTCTCTTCCTCTTCCACCTTCCATTACAGGGCCTTCCCCTTCATTCCCTGCTCTCTTTCCCACTTTATTTTACCTCCTTCTCTTCTTATTCTGTCAACCTCGATTGCCTTCCCTTTTTCGGTTTTGTGGAATTCTCGATTCCTGATGCCTTACAATCAGGTACCCATTTCTGAATTTTGTTTGCCATTGTTGGAATATGCGATGGATTTTCATCNCGGAGTCTCAACTGCCTCTCCCTTTTCTTGTTTCTTGTAATTTACCTCGTTCCCTTACTCTCGCAGCCTCAATTGCCTTCCCTTTTCTTGTTTCTTGTCATTTACCTCGTTCCCTTACTCTGTCAGCCTCAATTGCCTTTTCTTTTCTTGTTTATTGTAATTCTCGATTCCCTTTCCCCGTTTCTTGTGATTCTCGATTCATCATGCCTTACAATACTCATTTCTTTTGATGAATTTTGTTCACCATTGTAATTCAATGAATTCATCTCTATATTTTTGTATTTGAGTATCTTTGTTTGTTTTTGTTGATGCTTAGGTATTCACGATCGTCTGGTTTTGA

mRNA sequence

ATGTCGGCGCCGCATAGGATTTTCCGTGCTATAACTCTTCCTCTCCCTCGTTTCTCTTCCTCTTCCACCTTCCATTACAGGGCCTTCCCCTTCATTCCCTGCTCTCTTTCCCACTTTATTTTACCTCCTTCTCTTCTTATTCTGTCAACCTCGATTGCCTTCCCTTTTTCGGTTTTGTGGAATTCTCGATTCCTGATGCCTTACAATCAGGTATTCACGATCGTCTGGTTTTGA

Coding sequence (CDS)

ATGTCGGCGCCGCATAGGATTTTCCGTGCTATAACTCTTCCTCTCCCTCGTTTCTCTTCCTCTTCCACCTTCCATTACAGGGCCTTCCCCTTCATTCCCTGCTCTCTTTCCCACTTTATTTTACCTCCTTCTCTTCTTATTCTGTCAACCTCGATTGCCTTCCCTTTTTCGGTTTTGTGGAATTCTCGATTCCTGATGCCTTACAATCAGGTATTCACGATCGTCTGGTTTTGA

Protein sequence

MSAPHRIFRAITLPLPRFSSSSTFHYRAFPFIPCSLSHFILPPSLLILSTSIAFPFSVLWNSRFLMPYNQVFTIVWF
Homology
BLAST of Cp4.1LG20g09160 vs. NCBI nr
Match: XP_023519581.1 (tRNA ligase 1 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023519582.1 tRNA ligase 1 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 141 bits (356), Expect = 6.15e-37
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0

Query: 1  MSAPHRIFRAITLPLPRFSSSSTFHYRAFPFIPCSLSHFILPPSLLILSTSIAFPFSVLW 60
          MSAPHRIFRAITLPLPRFSSSSTFHYRAFPFIPCSLSHFILPPSLLILSTSIAFPFSVLW
Sbjct: 1  MSAPHRIFRAITLPLPRFSSSSTFHYRAFPFIPCSLSHFILPPSLLILSTSIAFPFSVLW 60

Query: 61 NSRFLMPYNQ 70
          NSRFLMPYNQ
Sbjct: 61 NSRFLMPYNQ 70

BLAST of Cp4.1LG20g09160 vs. NCBI nr
Match: KAG6583500.1 (tRNA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 78.2 bits (191), Expect = 1.21e-14
Identity = 45/70 (64.29%), Postives = 52/70 (74.29%), Query Frame = 0

Query: 1  MSAPHRIFRAITLPLPRFSSSSTFHYRAFPFIPCSLSHFILPPSLLILSTSIAFPFSVLW 60
          MSA +RIF AITLPL   SSS   HYRAFPF+ CSLSHFIL PSL + ++   FPF+V  
Sbjct: 1  MSATYRIFCAITLPL---SSSPALHYRAFPFVSCSLSHFILHPSLTLPAS--VFPFTVSR 60

Query: 61 NSRFLMPYNQ 70
          +SRF MPYNQ
Sbjct: 61 DSRFTMPYNQ 65

BLAST of Cp4.1LG20g09160 vs. NCBI nr
Match: XP_022970408.1 (tRNA ligase 1 [Cucurbita maxima])

HSP 1 Score: 75.9 bits (185), Expect = 7.89e-14
Identity = 46/71 (64.79%), Postives = 52/71 (73.24%), Query Frame = 0

Query: 1  MSAPHRIFRAITLPLPRFSSSSTFHYRAF-PFIPCSLSHFILPPSLLILSTSIAFPFSVL 60
          MSAPHRIF AITLP  R S SS F+YR F PFIP S SH IL PSL I + SI+FP +V 
Sbjct: 1  MSAPHRIFCAITLPRHRLSYSSAFNYRVFFPFIPYSFSHRILSPSLTI-TDSISFPSTVS 60

Query: 61 WNSRFLMPYNQ 70
           + RF+MPYNQ
Sbjct: 61 SDFRFMMPYNQ 70

BLAST of Cp4.1LG20g09160 vs. NCBI nr
Match: KAG7019255.1 (tRNA ligase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 75.1 bits (183), Expect = 1.47e-13
Identity = 44/70 (62.86%), Postives = 51/70 (72.86%), Query Frame = 0

Query: 1  MSAPHRIFRAITLPLPRFSSSSTFHYRAFPFIPCSLSHFILPPSLLILSTSIAFPFSVLW 60
          MSA +RIF AITLPL   SSS   H RAFPF+ CSLSHFIL PSL + ++   FPF+V  
Sbjct: 1  MSATYRIFCAITLPL---SSSPALHSRAFPFVSCSLSHFILHPSLTLPAS--VFPFTVCR 60

Query: 61 NSRFLMPYNQ 70
          +SRF MPYNQ
Sbjct: 61 DSRFTMPYNQ 65

BLAST of Cp4.1LG20g09160 vs. NCBI nr
Match: XP_022964893.1 (tRNA ligase 1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 75.1 bits (183), Expect = 1.47e-13
Identity = 44/70 (62.86%), Postives = 51/70 (72.86%), Query Frame = 0

Query: 1  MSAPHRIFRAITLPLPRFSSSSTFHYRAFPFIPCSLSHFILPPSLLILSTSIAFPFSVLW 60
          MSA +RIF AITLPL   SSS   H RAFPF+ CSLSHFIL PSL + ++   FPF+V  
Sbjct: 1  MSATYRIFCAITLPL---SSSPALHSRAFPFVSCSLSHFILHPSLTLPAS--VFPFTVCR 60

Query: 61 NSRFLMPYNQ 70
          +SRF MPYNQ
Sbjct: 61 DSRFTMPYNQ 65

BLAST of Cp4.1LG20g09160 vs. ExPASy TrEMBL
Match: A0A6J1I3R5 (tRNA ligase 1 OS=Cucurbita maxima OX=3661 GN=LOC111469400 PE=4 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 3.82e-14
Identity = 46/71 (64.79%), Postives = 52/71 (73.24%), Query Frame = 0

Query: 1  MSAPHRIFRAITLPLPRFSSSSTFHYRAF-PFIPCSLSHFILPPSLLILSTSIAFPFSVL 60
          MSAPHRIF AITLP  R S SS F+YR F PFIP S SH IL PSL I + SI+FP +V 
Sbjct: 1  MSAPHRIFCAITLPRHRLSYSSAFNYRVFFPFIPYSFSHRILSPSLTI-TDSISFPSTVS 60

Query: 61 WNSRFLMPYNQ 70
           + RF+MPYNQ
Sbjct: 61 SDFRFMMPYNQ 70

BLAST of Cp4.1LG20g09160 vs. ExPASy TrEMBL
Match: A0A6J1HM92 (tRNA ligase 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464863 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 7.12e-14
Identity = 44/70 (62.86%), Postives = 51/70 (72.86%), Query Frame = 0

Query: 1  MSAPHRIFRAITLPLPRFSSSSTFHYRAFPFIPCSLSHFILPPSLLILSTSIAFPFSVLW 60
          MSA +RIF AITLPL   SSS   H RAFPF+ CSLSHFIL PSL + ++   FPF+V  
Sbjct: 1  MSATYRIFCAITLPL---SSSPALHSRAFPFVSCSLSHFILHPSLTLPAS--VFPFTVCR 60

Query: 61 NSRFLMPYNQ 70
          +SRF MPYNQ
Sbjct: 61 DSRFTMPYNQ 65

BLAST of Cp4.1LG20g09160 vs. ExPASy TrEMBL
Match: A0A5A7UUA9 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold778G00320 PE=4 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.88e-08
Identity = 42/72 (58.33%), Postives = 46/72 (63.89%), Query Frame = 0

Query: 1  MSAPHRIFRAITLPLPRFSSSSTFHYRAFPFIPCSLSHFILPPSLLILS-TSIAFPFSVL 60
          MSA  RIF A  LP P FSSS    Y+ FPFI   LSHFILP SL +   TS  FP S  
Sbjct: 1  MSALQRIFYAKILPHPPFSSS----YKVFPFICHPLSHFILPRSLTLAPLTSSPFPLSC- 60

Query: 61 WNSRFLMPYNQV 71
           +SRF+MPYNQV
Sbjct: 61 -DSRFVMPYNQV 66

BLAST of Cp4.1LG20g09160 vs. ExPASy TrEMBL
Match: A0A0A0LVA8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G087440 PE=4 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 2.27e-08
Identity = 39/70 (55.71%), Postives = 45/70 (64.29%), Query Frame = 0

Query: 1  MSAPHRIFRAITLPLPRFSSSSTFHYRAFPFIPCSLSHFILPPSLLILSTSIAFPFSVLW 60
          MSA  RIF A TLP P FSSS    YR FPFI   LSH+ILP SL  L+   + P  +  
Sbjct: 1  MSALQRIFCAKTLPHPPFSSS----YRVFPFISHPLSHYILPRSLT-LAPLTSSPLPISC 60

Query: 61 NSRFLMPYNQ 70
          +SRF+MPYNQ
Sbjct: 61 DSRFVMPYNQ 65

BLAST of Cp4.1LG20g09160 vs. ExPASy TrEMBL
Match: A0A1S3CK49 (uncharacterized protein LOC103501711 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501711 PE=4 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 6.59e-08
Identity = 41/71 (57.75%), Postives = 45/71 (63.38%), Query Frame = 0

Query: 1  MSAPHRIFRAITLPLPRFSSSSTFHYRAFPFIPCSLSHFILPPSLLILS-TSIAFPFSVL 60
          MSA  RIF A  LP P FSSS    Y+ FPFI   LSHFILP SL +   TS  FP S  
Sbjct: 1  MSALQRIFYAKILPHPPFSSS----YKVFPFICHPLSHFILPRSLTLAPLTSSPFPLSC- 60

Query: 61 WNSRFLMPYNQ 70
           +SRF+MPYNQ
Sbjct: 61 -DSRFVMPYNQ 65

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_023519581.16.15e-37100.00tRNA ligase 1 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023519582.1 tRNA ligas... [more]
KAG6583500.11.21e-1464.29tRNA ligase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022970408.17.89e-1464.79tRNA ligase 1 [Cucurbita maxima][more]
KAG7019255.11.47e-1362.86tRNA ligase 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022964893.11.47e-1362.86tRNA ligase 1 isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1I3R53.82e-1464.79tRNA ligase 1 OS=Cucurbita maxima OX=3661 GN=LOC111469400 PE=4 SV=1[more]
A0A6J1HM927.12e-1462.86tRNA ligase 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464863 PE=4 SV=1[more]
A0A5A7UUA91.88e-0858.33Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A0A0LVA82.27e-0855.71Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G087440 PE=4 SV=1[more]
A0A1S3CK496.59e-0857.75uncharacterized protein LOC103501711 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG20g09160.1Cp4.1LG20g09160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016874 ligase activity