Cp4.1LG20g06900 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG20g06900
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Calcium-binding EF-hand family protein
Location	Cp4.1LG20: 5313220 .. 5316043 (-)
RNA-Seq Expression	Cp4.1LG20g06900
Synteny	Cp4.1LG20g06900

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTTCATTCTGAATCCCACCGAAACTCTGAGCCGATAAACACAAAACCGAACGAAAGTTGCTTTCTCTGCCACCGTTTCATCCCAAAAGAAGCTCCCCGATTCGATTACCCCTTCTGATTCTTTACTTTTTCTGATCCCATCTTCTGTTCATCCTCTCTTCTTTTTGGCCATCAATGGCATCCTTCTTTGGTAGATTCACATCCCATTCCAGTTCCAGCTCCAATTCCAAACCCCATGCTCCATCTGCACCGTACGCGCCATCGGCGCCGGAAGCCCACGGCCATGAACATTCTCATGGGACCGCTTCGCCGTATTTCTCCGAAAACCATCCGCCGCAGCCTTATGGGTCTAATTATGGTGGGGTTTCTTCATATGGGTCTTATGGTTTTCCACCTGGGACCCCCCCTGAGGTAATTAGGAGCTTCCAAATGGTGGATAGAGATGGAAGTGGGTTCATTGATGAGAATGAATTGCAGCAAGCTCTTTCTTCTGGCTACCAGAGATTTAGTCTTAGAACCGTTCGTTTGCTCATTTTCTTGTTCAGAAATCCGGTTGATTCCTCCAGAATGGGTGAGTTTCTTATTATCTGTTCAATCTTGGATGCAATCAAGCTCGGTTGAGATCGTTATTTAGACTGAAACCTCTGTTTAATTTTGTCCTGTTCGTTGCCTTTCTTGGAGGCTCAATGGGCAACGATTGTCTTGTCTGCCCTGTATCGAACTAAGGGGAATAGATTGTGAGTAAATGTAAATTGAAAATGAAAACCCATGTTGTTAAATTTGAATAGGAATTCAGCTTCATTCTATATGGAGTCCTCAATTTTGGTTGTTTCGAATGAGTTTGGTGGTGCCTTTTACTTAACAATGTGTGTCTGAATTGGCTGTTGCAGGGCCTAATGAGTTTACAGCTTTGTGGAACTGTCTTGGACAATGGCGTGTGAGTAATGTTGTGATTGTGTGGTTTCCCTCTCTTTTCTTCATTTTGCCCTCATAATTTATCTGCTTTTGCTGAAACTTCTTAAAGTGATGATGACAATGTGGAACTTCTGAGTAGATTGTTGGTTTTAAATGTTCTTATGTGGACTGTTGTGTGAAAAAATAATTGTTGGGTGTTAACCGACCAATTCCACTACCAGTAGATATTGTCCTTTTTGTGCTTTCCCTTTTGGATTTCCCCTCAAGATTTTAAAATGCGTATGCTAGAGAGAGGTTTCCACACCCTTTTAAAGAATGTTTCGTTTCCCTCTCCAACCGATGTGGGATCTCACAATGAATGGTATCAGAGCCAGATATCGGGCGGTGTGCCAGTAAGGACAGTGAGCTCGAAGGAGAGTGGACACCGGATGGTGTGCTAGCAAGGATGTCAGACCCTGAAGGGGAGTGGATTGTGAGGTCCCACATCGATTGGAGAGAGGAACAAATGACAGTGAGGACACTGGGTCCCGAAAGAGGCGGATTGTGAGATCCTACATCGGTTGGAGAGGGGAACAAAGCTTTCTTTATAAGGGTGTGGAAACCTCTCTCTAGCATACGCGCTTTAAAACCTTGAAGGGAAGCTCGGAAGGTAAAGTCCAAAGAGGACAATTTCTGCTAGTGGTGGACTTGGGCTGTTTTAGTTGATCCAATTTGTTGAAGCCATCCAGTTTATGTGTAATATCATTAAAGAAATTGGTAGAACTTAGAACTTAGTTCAAAACGTGATTTTTCTGCATTTTGTTCTTTCATTCAGAAAGCAAAACTCTCTTGCTTCTACAACTACTTTCAGAACAATATAGAAGCACATGAAACATGTAAATTTAAGGATGTTTTTAGGATAAGGCTTTCTAGAGGTTTGACGAAAGAGGGTAGATAAGAAGAAAACGAAACGACAGAGCAACCTTTTAATTTAATCAGGACAGTTCCAGGTTGTATATCGAACACGTTCGAGTTTTATTTGCCTTTTCCATTCCTTTTATTTATTTATTTTCAGAATTCCTTCAGACCTTCGAGACTAAAGAACTCTCATTTTCAGGGCACGTTCGAGCGATATGATCGAGATAGAAGTGGGAAAATTGATGCATTAGAAATGAGGGATGCACTTTATGGTCTTGGCTATGCAGTCCCGTCTTCAGTCCTCGAACTTCTAATATCACTTTACGATGATCGAAGTGGGCGTCGAGTTGAATTTAACTTCGACAGTTTCGTCGAGTAAGTGTTTGAGCATAGTTGAATACTTCTAGCTTCTTATGAGATATACTTACATTTCTGGAATTGTCTATTTCTTGTCTGCAGGTGTGGCATGATAGTAAAGGTAAGACTTCAGACCTCTTACTCCCGGTTTTACTAATATCGTCACGTTGCTCGACTAAATAATACTATCGATAAATCTTGTTGAAGTAATCGAATGTATAAGTAAAACTGAAAGCGTTTGTTGTTCATGGGTATCATTATCATATTGTTAGTAGCAGCACATGGTAAGTGATATCATTTCTAAACCGATCCGTGCAAAATTACAGGGCTTGACCGAGAAATTCAAGGAGAAGGATAGGCACTACACAGGTTCGGCTACGCTGACGTACGAAGATTTCATGTCAACAATCCTCCCATTTCTTGTGTCTTACAGTTAAAAGGCCTCAAAAGTAGACAAAGTGTTCATTTTAGGACGTTTGGTTTAGAGCAAAAAGGTTTTTGTTTCAGCTGCGTAGAGATATGTTTTGGAAGTTGTTTTTTCTTTATCAAAATTCTCCTCGCTATCCTATGCATGGCGTTACCTTTTTTTTTTTTTTTTTTTTTTTTGCGCCGAAAACATTCATTTTAAATAGTTCATAAATGATGTCATGAACT

mRNA sequence

CTTTTCATTCTGAATCCCACCGAAACTCTGAGCCGATAAACACAAAACCGAACGAAAGTTGCTTTCTCTGCCACCGTTTCATCCCAAAAGAAGCTCCCCGATTCGATTACCCCTTCTGATTCTTTACTTTTTCTGATCCCATCTTCTGTTCATCCTCTCTTCTTTTTGGCCATCAATGGCATCCTTCTTTGGTAGATTCACATCCCATTCCAGTTCCAGCTCCAATTCCAAACCCCATGCTCCATCTGCACCGTACGCGCCATCGGCGCCGGAAGCCCACGGCCATGAACATTCTCATGGGACCGCTTCGCCGTATTTCTCCGAAAACCATCCGCCGCAGCCTTATGGGTCTAATTATGGTGGGGTTTCTTCATATGGGTCTTATGGTTTTCCACCTGGGACCCCCCCTGAGGTAATTAGGAGCTTCCAAATGGTGGATAGAGATGGAAGTGGGTTCATTGATGAGAATGAATTGCAGCAAGCTCTTTCTTCTGGCTACCAGAGATTTAGTCTTAGAACCGTTCGTTTGCTCATTTTCTTGTTCAGAAATCCGGTTGATTCCTCCAGAATGGGGCCTAATGAGTTTACAGCTTTGTGGAACTGTCTTGGACAATGGCGTAATTCCTTCAGACCTTCGAGACTAAAGAACTCTCATTTTCAGGGCACGTTCGAGCGATATGATCGAGATAGAAGTGGGAAAATTGATGCATTAGAAATGAGGGATGCACTTTATGGTCTTGGCTATGCAGTCCCGTCTTCAGTCCTCGAACTTCTAATATCACTTTACGATGATCGAAGTGGGCGTCGAGTTGAATTTAACTTCGACAGTTTCGTCGAGTGTGGCATGATAGTAAAGGGCTTGACCGAGAAATTCAAGGAGAAGGATAGGCACTACACAGGTTCGGCTACGCTGACGTACGAAGATTTCATGTCAACAATCCTCCCATTTCTTGTGTCTTACAGTTAAAAGGCCTCAAAAGTAGACAAAGTGTTCATTTTAGGACGTTTGGTTTAGAGCAAAAAGGTTTTTGTTTCAGCTGCGTAGAGATATGTTTTGGAAGTTGTTTTTTCTTTATCAAAATTCTCCTCGCTATCCTATGCATGGCGTTACCTTTTTTTTTTTTTTTTTTTTTTTTGCGCCGAAAACATTCATTTTAAATAGTTCATAAATGATGTCATGAACT

Coding sequence (CDS)

ATGGCATCCTTCTTTGGTAGATTCACATCCCATTCCAGTTCCAGCTCCAATTCCAAACCCCATGCTCCATCTGCACCGTACGCGCCATCGGCGCCGGAAGCCCACGGCCATGAACATTCTCATGGGACCGCTTCGCCGTATTTCTCCGAAAACCATCCGCCGCAGCCTTATGGGTCTAATTATGGTGGGGTTTCTTCATATGGGTCTTATGGTTTTCCACCTGGGACCCCCCCTGAGGTAATTAGGAGCTTCCAAATGGTGGATAGAGATGGAAGTGGGTTCATTGATGAGAATGAATTGCAGCAAGCTCTTTCTTCTGGCTACCAGAGATTTAGTCTTAGAACCGTTCGTTTGCTCATTTTCTTGTTCAGAAATCCGGTTGATTCCTCCAGAATGGGGCCTAATGAGTTTACAGCTTTGTGGAACTGTCTTGGACAATGGCGTAATTCCTTCAGACCTTCGAGACTAAAGAACTCTCATTTTCAGGGCACGTTCGAGCGATATGATCGAGATAGAAGTGGGAAAATTGATGCATTAGAAATGAGGGATGCACTTTATGGTCTTGGCTATGCAGTCCCGTCTTCAGTCCTCGAACTTCTAATATCACTTTACGATGATCGAAGTGGGCGTCGAGTTGAATTTAACTTCGACAGTTTCGTCGAGTGTGGCATGATAGTAAAGGGCTTGACCGAGAAATTCAAGGAGAAGGATAGGCACTACACAGGTTCGGCTACGCTGACGTACGAAGATTTCATGTCAACAATCCTCCCATTTCTTGTGTCTTACAGTTAA

Protein sequence

MASFFGRFTSHSSSSSNSKPHAPSAPYAPSAPEAHGHEHSHGTASPYFSENHPPQPYGSNYGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLIFLFRNPVDSSRMGPNEFTALWNCLGQWRNSFRPSRLKNSHFQGTFERYDRDRSGKIDALEMRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMSTILPFLVSYS

Homology

[Close]

BLAST of Cp4.1LG20g06900 vs. ExPASy Swiss-Prot
Match: Q9ZQH1 (Probable calcium-binding protein CML48 OS=Arabidopsis thaliana OX=3702 GN=CML48 PE=2 SV=2)

HSP 1 Score: 213.8 bits (543), Expect = 2.4e-54
Identity = 118/251 (47.01%), Postives = 163/251 (64.94%), Query Frame = 0


Query: 16  SNSKPHAPSAPYAPSA--PEAHGHEHSHGTASPYFSENHPPQPYGSNYGGVSSYGSYGFP 75
           S S  +APSAP  P +   + H  E  +  A P    ++ P    S+Y G+       F 
Sbjct: 2   SYSNAYAPSAPELPESFVQQQHDGESRYTYAYP----SYQPTQQFSSYSGM-------FS 61

Query: 76  PGTPPEVIRSFQMVDRDGSGFIDENELQQALS-SGYQRFSLRTVRLLIFLFRNPVDS-SR 135
           P T PE++RSF+  DR+ SGF++E+EL+QALS SGY   S RT+RLL+F+++ PVDS  R
Sbjct: 62  PETHPEIVRSFESADRNRSGFLEESELRQALSLSGYDGISNRTIRLLLFIYKIPVDSLLR 121

Query: 136 MGPNEFTALWNCLGQWRNSFRPSRLKNSHFQGTFERYDRDRSGKIDALEMRDALYGLGYA 195
           +GP E+  LWNCL QWR                F RYDRDRSGK+++ ++RDA Y LG  
Sbjct: 122 LGPKEYVELWNCLAQWR--------------AIFNRYDRDRSGKMNSTQLRDAFYNLGCV 181

Query: 196 VPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDF 255
           +P+SV +L++S +DD +G+ V+  FDSF+ECGMIVKGLTEKF+E D  YTG ATL+Y+ F
Sbjct: 182 LPTSVHQLIVSQFDDGTGKTVDLCFDSFLECGMIVKGLTEKFRENDPGYTGYATLSYDVF 227

Query: 256 MSTILPFLVSY 263
           M  ++PF+ +Y
Sbjct: 242 MLMVIPFIATY 227

[Close]

BLAST of Cp4.1LG20g06900 vs. ExPASy Swiss-Prot
Match: Q2QY10 (Calcium-binding protein CBP OS=Oryza sativa subsp. japonica OX=39947 GN=CBP PE=2 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.0e-52
Identity = 120/261 (45.98%), Postives = 157/261 (60.15%), Query Frame = 0


Query: 19  KPHAPSAPY--------APSAPEAHGHEHSHG---TASPYFSENHPPQPYGSNYGGVSSY 78
           K  + SAPY        AP + + +G    +G   ++ PY +    P P  + YG    Y
Sbjct: 47  KTSSSSAPYYGGGGGYGAPPSTQPYGSGGGYGAPPSSQPYGAPYGAPPPSSAPYGAPGGY 106

Query: 79  GS-------YGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 138
           GS         FPPGT P V+  FQ  DRDGSG ID+ ELQ ALS   Q FSLRTV LL+
Sbjct: 107 GSPFASLVPSAFPPGTDPNVVACFQAADRDGSGMIDDKELQSALSGYSQSFSLRTVHLLM 166

Query: 139 FLFRNPVDSSRMGPNEFTALWNCLGQWRNSFRPSRLKNSHFQGTFERYDRDRSGKIDALE 198
           +LF N  +  ++GP EFT+++  L  WR+               FER+DRDRSGKIDA E
Sbjct: 167 YLFTN-TNVRKIGPKEFTSVFYSLQNWRS--------------IFERFDRDRSGKIDATE 226

Query: 199 MRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHY 258
           +RDAL  LGY+V  +VL+LL+S +D   G+     +D+F+EC + VKGLTEKFKEKD  +
Sbjct: 227 LRDALLSLGYSVSPTVLDLLVSKFDKTGGKNKAIEYDNFIECCLTVKGLTEKFKEKDTAF 286

Query: 259 TGSATLTYEDFMSTILPFLVS 262
           +GSAT TYE FM T+LPFL++
Sbjct: 287 SGSATFTYEAFMLTVLPFLIA 292

[Close]

BLAST of Cp4.1LG20g06900 vs. ExPASy Swiss-Prot
Match: Q9FYE4 (Probable calcium-binding protein CML50 OS=Arabidopsis thaliana OX=3702 GN=CML50 PE=2 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 8.5e-52
Identity = 118/251 (47.01%), Postives = 153/251 (60.96%), Query Frame = 0


Query: 29  PSAPEAHGHEHS----HGTASPYFSENHPPQP----YGSNYGGV---SSYGS-------Y 88
           PS P  HG  +     HG +        PP+P    +G  YGG    +SYGS        
Sbjct: 119 PSQPSGHGGGYGATPPHGVSDYGSYGGAPPRPASSGHGGGYGGYPPQASYGSPFASLIPS 178

Query: 89  GFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLIFLFRNPVDSS 148
           GF PGT P ++  FQ  D+DGSGFID+ ELQ ALSS  QRFS+RTV LL++LF N  ++ 
Sbjct: 179 GFAPGTDPNIVACFQAADQDGSGFIDDKELQGALSSYQQRFSMRTVHLLMYLFTNS-NAM 238

Query: 149 RMGPNEFTALWNCLGQWRNSFRPSRLKNSHFQGTFERYDRDRSGKIDALEMRDALYGLGY 208
           ++GP EFTAL+  L  WR+               FER D+DRSG+ID  E+RDAL  LG+
Sbjct: 239 KIGPKEFTALFYSLQNWRS--------------IFERSDKDRSGRIDVNELRDALLSLGF 298

Query: 209 AVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYED 262
           +V   VL+LL+S +D   G+     +D+F+EC + VKGLTEKFKEKD  Y+GSAT  YE 
Sbjct: 299 SVSPVVLDLLVSKFDKSGGKNRAIEYDNFIECCLTVKGLTEKFKEKDTAYSGSATFNYES 354

[Close]

BLAST of Cp4.1LG20g06900 vs. ExPASy Swiss-Prot
Match: Q8W4L0 (Probable calcium-binding protein CML49 OS=Arabidopsis thaliana OX=3702 GN=CML49 PE=2 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.7e-47
Identity = 118/268 (44.03%), Postives = 152/268 (56.72%), Query Frame = 0


Query: 19  KPHAPSAPYAPSAPEAHGHEHSHGTASPYFSENHPPQP--YGSNYGGV------------ 78
           KPH   A Y   +P  +G   S G  S Y      PQ   +G  YGG             
Sbjct: 86  KPH--GASYGSPSPGGYGAHPSSG-PSDYGGYGGAPQQSGHGGGYGGAPQQSGHGGGYGA 145

Query: 79  ----SSYGS-------YGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSL 138
               +SYGS         FPPGT P ++  FQ  DRD SGFID+ ELQ ALSS  Q FS+
Sbjct: 146 PPPQASYGSPFASLVPSAFPPGTDPNIVACFQAADRDNSGFIDDKELQGALSSYNQSFSI 205

Query: 139 RTVRLLIFLFRNPVDSSRMGPNEFTALWNCLGQWRNSFRPSRLKNSHFQGTFERYDRDRS 198
           RTV LL++LF N  +  ++GP EFT+L+  L  WR+               FER+D+DRS
Sbjct: 206 RTVHLLMYLFTNS-NVRKIGPKEFTSLFFSLQNWRS--------------IFERFDKDRS 265

Query: 199 GKIDALEMRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKF 258
           G+ID  E+RDAL  LG++V   +L+LL+S +D   GR     +D+F+EC + VKGLTEKF
Sbjct: 266 GRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIECCLTVKGLTEKF 325

Query: 259 KEKDRHYTGSATLTYEDFMSTILPFLVS 262
           KEKD   +GSA   YE+FM T+LPFLV+
Sbjct: 326 KEKDTALSGSAIFNYENFMLTVLPFLVA 335

[Close]

BLAST of Cp4.1LG20g06900 vs. ExPASy Swiss-Prot
Match: Q5PQ53 (Peflin OS=Xenopus laevis OX=8355 GN=pef1 PE=1 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.0e-25
Identity = 78/233 (33.48%), Postives = 122/233 (52.36%), Query Frame = 0


Query: 22  APSAPYAPSAPEAHGHEHSHGTASPYFSENHPPQPYGSNYGGVSSYGSYGFPPGTPPEVI 81
           AP AP  P   +A G  +S   ++PY S+ H     G+  G +        PPG  PE  
Sbjct: 68  APGAPGGPYGGQAPGGPYSVPGSTPYGSQQHGSYGQGAPAGNI--------PPGVDPEAF 127

Query: 82  RSFQMVDRDGSGFIDENELQQAL-SSGYQRFSLRTVRLLIFLFRNPVDSSRMGPNEFTAL 141
             FQ VD D SG+I   EL+QAL ++ +  F+  T  +++ +F +  +S R+    F+AL
Sbjct: 128 SWFQTVDTDHSGYISLKELKQALVNTNWSSFNDETCTMMMNMF-DKSNSGRIDMFGFSAL 187

Query: 142 WNCLGQWRNSFRPSRLKNSHFQGTFERYDRDRSGKIDALEMRDALYGLGYAVPSSVLELL 201
           W  + QWRN               F++YDRDRSG I+  E+  AL  +GY +    ++++
Sbjct: 188 WRFIQQWRN--------------LFQQYDRDRSGSINQGELHQALCQMGYQLSPQFVQIV 247

Query: 202 ISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMS 254
           +S Y  RS  +     D F++    ++ +TE F+EKD    G+A L+YEDF++
Sbjct: 248 MSRYAQRSA-QPGLQLDRFIQICTQLQSMTEAFREKDTGQIGTAKLSYEDFIT 276

[Close]

BLAST of Cp4.1LG20g06900 vs. NCBI nr
Match: XP_023519854.1 (probable calcium-binding protein CML48 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 495 bits (1275), Expect = 2.16e-176
Identity = 249/263 (94.68%), Postives = 249/263 (94.68%), Query Frame = 0


Query: 1   MASFFGRFTSHSSSSSNSKPHAPSAPYAPSAPEAHGHEHSHGTASPYFSENHPPQPYGSN 60
           MASFFGRFTSHSSSSSNSKPHAPSAPYAPSAPEAHGHEHSHGTASPYFSENHPPQPYGSN
Sbjct: 1   MASFFGRFTSHSSSSSNSKPHAPSAPYAPSAPEAHGHEHSHGTASPYFSENHPPQPYGSN 60

Query: 61  YGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 120
           YGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI
Sbjct: 61  YGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 120

Query: 121 FLFRNPVDSSRMGPNEFTALWNCLGQWRNSFRPSRLKNSHFQGTFERYDRDRSGKIDALE 180
           FLFRNPVDSSRMGPNEFTALWNCLGQWR              GTFERYDRDRSGKIDALE
Sbjct: 121 FLFRNPVDSSRMGPNEFTALWNCLGQWR--------------GTFERYDRDRSGKIDALE 180

Query: 181 MRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHY 240
           MRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHY
Sbjct: 181 MRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHY 240

Query: 241 TGSATLTYEDFMSTILPFLVSYS 263
           TGSATLTYEDFMSTILPFLVSYS
Sbjct: 241 TGSATLTYEDFMSTILPFLVSYS 249

[Close]

BLAST of Cp4.1LG20g06900 vs. NCBI nr
Match: KAG6583656.1 (putative calcium-binding protein CML48, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028448.1 putative calcium-binding protein CML48 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 492 bits (1266), Expect = 5.08e-175
Identity = 247/263 (93.92%), Postives = 248/263 (94.30%), Query Frame = 0


Query: 1   MASFFGRFTSHSSSSSNSKPHAPSAPYAPSAPEAHGHEHSHGTASPYFSENHPPQPYGSN 60
           MASFFGRFTSHSSSSSNSKPHAPSAPYAPSAPE HGHEHSHGTASPYFSE+HPPQPYGSN
Sbjct: 1   MASFFGRFTSHSSSSSNSKPHAPSAPYAPSAPEVHGHEHSHGTASPYFSEHHPPQPYGSN 60

Query: 61  YGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 120
           YGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI
Sbjct: 61  YGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 120

Query: 121 FLFRNPVDSSRMGPNEFTALWNCLGQWRNSFRPSRLKNSHFQGTFERYDRDRSGKIDALE 180
           FLFRNPVDSSRMGPNEFTALWNCLGQWR              GTFERYDRDRSGKIDALE
Sbjct: 121 FLFRNPVDSSRMGPNEFTALWNCLGQWR--------------GTFERYDRDRSGKIDALE 180

Query: 181 MRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHY 240
           MRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHY
Sbjct: 181 MRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHY 240

Query: 241 TGSATLTYEDFMSTILPFLVSYS 263
           TGSATLTYEDFMSTILPFLVSYS
Sbjct: 241 TGSATLTYEDFMSTILPFLVSYS 249

[Close]

BLAST of Cp4.1LG20g06900 vs. NCBI nr
Match: XP_022932202.1 (probable calcium-binding protein CML48 [Cucurbita moschata])

HSP 1 Score: 489 bits (1260), Expect = 4.17e-174
Identity = 246/263 (93.54%), Postives = 247/263 (93.92%), Query Frame = 0


Query: 1   MASFFGRFTSHSSSSSNSKPHAPSAPYAPSAPEAHGHEHSHGTASPYFSENHPPQPYGSN 60
           MASFFGRFTSHSSSSSNSKPHAPSAPYAPSAPE HGHEHSHGTASPYFSE+HPPQPYGSN
Sbjct: 1   MASFFGRFTSHSSSSSNSKPHAPSAPYAPSAPEVHGHEHSHGTASPYFSEHHPPQPYGSN 60

Query: 61  YGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 120
           YG VSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI
Sbjct: 61  YGAVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 120

Query: 121 FLFRNPVDSSRMGPNEFTALWNCLGQWRNSFRPSRLKNSHFQGTFERYDRDRSGKIDALE 180
           FLFRNPVDSSRMGPNEFTALWNCLGQWR              GTFERYDRDRSGKIDALE
Sbjct: 121 FLFRNPVDSSRMGPNEFTALWNCLGQWR--------------GTFERYDRDRSGKIDALE 180

Query: 181 MRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHY 240
           MRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHY
Sbjct: 181 MRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHY 240

Query: 241 TGSATLTYEDFMSTILPFLVSYS 263
           TGSATLTYEDFMSTILPFLVSYS
Sbjct: 241 TGSATLTYEDFMSTILPFLVSYS 249

[Close]

BLAST of Cp4.1LG20g06900 vs. NCBI nr
Match: XP_022970340.1 (probable calcium-binding protein CML48 [Cucurbita maxima])

HSP 1 Score: 477 bits (1228), Expect = 3.15e-169
Identity = 241/263 (91.63%), Postives = 244/263 (92.78%), Query Frame = 0


Query: 1   MASFFGRFTSHSSSSSNSKPHAPSAPYAPSAPEAHGHEHSHGTASPYFSENHPPQPYGSN 60
           MASFFGRFTSHSSSSSNS  HAPSAPYAPSAPEAHGHEHSH TASPYFSE+HPPQPYGSN
Sbjct: 1   MASFFGRFTSHSSSSSNSNSHAPSAPYAPSAPEAHGHEHSHETASPYFSEHHPPQPYGSN 60

Query: 61  YGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 120
           YGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI
Sbjct: 61  YGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 120

Query: 121 FLFRNPVDSSRMGPNEFTALWNCLGQWRNSFRPSRLKNSHFQGTFERYDRDRSGKIDALE 180
           FLFRNPVDSSRMGPNEFTALWNCLGQWR              GTFERYDRDRSGKIDALE
Sbjct: 121 FLFRNPVDSSRMGPNEFTALWNCLGQWR--------------GTFERYDRDRSGKIDALE 180

Query: 181 MRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHY 240
           MRDALYGLGYAVPSSVL+LLISLYDD+SGRRVEFNF SFVECGMIVKGLTEKFKEKDRHY
Sbjct: 181 MRDALYGLGYAVPSSVLKLLISLYDDQSGRRVEFNFGSFVECGMIVKGLTEKFKEKDRHY 240

Query: 241 TGSATLTYEDFMSTILPFLVSYS 263
           TGSA LTYEDFMSTILPFLVSYS
Sbjct: 241 TGSAMLTYEDFMSTILPFLVSYS 249

[Close]

BLAST of Cp4.1LG20g06900 vs. NCBI nr
Match: XP_022967101.1 (probable calcium-binding protein CML48 [Cucurbita maxima])

HSP 1 Score: 447 bits (1151), Expect = 1.71e-157
Identity = 224/263 (85.17%), Postives = 239/263 (90.87%), Query Frame = 0


Query: 1   MASFFGRFTSHSSSSSNSKPHAPSAPYAPSAPEAHGHEHSHGTASPYFSENHPPQPYGSN 60
           MASFFGRFTS+S+S+S+S  HAPSAP APS PE +GH +SHGTASPY+S+ H PQPYGSN
Sbjct: 1   MASFFGRFTSYSNSNSHSHSHAPSAPSAPSEPETYGHGNSHGTASPYYSQQHQPQPYGSN 60

Query: 61  YGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 120
           YGGVSSYGSYGFPPGT PEVIRSFQMVD+D SGFIDENELQ+ALSSGYQRFSLRTVRLLI
Sbjct: 61  YGGVSSYGSYGFPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLRTVRLLI 120

Query: 121 FLFRNPVDSSRMGPNEFTALWNCLGQWRNSFRPSRLKNSHFQGTFERYDRDRSGKIDALE 180
           FLFRNP+DSSRMGPNEFTALWNCLGQWR              GTFERYDRDRSG+IDALE
Sbjct: 121 FLFRNPIDSSRMGPNEFTALWNCLGQWR--------------GTFERYDRDRSGRIDALE 180

Query: 181 MRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHY 240
           MRDALYGLGYAVPSSVL+LLISLYDDRSGRRVE+NFDSFVECGMIVKGLTEKFKEKD+HY
Sbjct: 181 MRDALYGLGYAVPSSVLQLLISLYDDRSGRRVEYNFDSFVECGMIVKGLTEKFKEKDKHY 240

Query: 241 TGSATLTYEDFMSTILPFLVSYS 263
           TGSATLTYEDFMSTILPFLVSYS
Sbjct: 241 TGSATLTYEDFMSTILPFLVSYS 249

[Close]

BLAST of Cp4.1LG20g06900 vs. ExPASy TrEMBL
Match: A0A6J1EVZ6 (probable calcium-binding protein CML48 OS=Cucurbita moschata OX=3662 GN=LOC111438517 PE=4 SV=1)

HSP 1 Score: 489 bits (1260), Expect = 2.02e-174
Identity = 246/263 (93.54%), Postives = 247/263 (93.92%), Query Frame = 0


Query: 1   MASFFGRFTSHSSSSSNSKPHAPSAPYAPSAPEAHGHEHSHGTASPYFSENHPPQPYGSN 60
           MASFFGRFTSHSSSSSNSKPHAPSAPYAPSAPE HGHEHSHGTASPYFSE+HPPQPYGSN
Sbjct: 1   MASFFGRFTSHSSSSSNSKPHAPSAPYAPSAPEVHGHEHSHGTASPYFSEHHPPQPYGSN 60

Query: 61  YGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 120
           YG VSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI
Sbjct: 61  YGAVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 120

Query: 121 FLFRNPVDSSRMGPNEFTALWNCLGQWRNSFRPSRLKNSHFQGTFERYDRDRSGKIDALE 180
           FLFRNPVDSSRMGPNEFTALWNCLGQWR              GTFERYDRDRSGKIDALE
Sbjct: 121 FLFRNPVDSSRMGPNEFTALWNCLGQWR--------------GTFERYDRDRSGKIDALE 180

Query: 181 MRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHY 240
           MRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHY
Sbjct: 181 MRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHY 240

Query: 241 TGSATLTYEDFMSTILPFLVSYS 263
           TGSATLTYEDFMSTILPFLVSYS
Sbjct: 241 TGSATLTYEDFMSTILPFLVSYS 249

[Close]

BLAST of Cp4.1LG20g06900 vs. ExPASy TrEMBL
Match: A0A6J1I2L1 (probable calcium-binding protein CML48 OS=Cucurbita maxima OX=3661 GN=LOC111469333 PE=4 SV=1)

HSP 1 Score: 477 bits (1228), Expect = 1.52e-169
Identity = 241/263 (91.63%), Postives = 244/263 (92.78%), Query Frame = 0


Query: 1   MASFFGRFTSHSSSSSNSKPHAPSAPYAPSAPEAHGHEHSHGTASPYFSENHPPQPYGSN 60
           MASFFGRFTSHSSSSSNS  HAPSAPYAPSAPEAHGHEHSH TASPYFSE+HPPQPYGSN
Sbjct: 1   MASFFGRFTSHSSSSSNSNSHAPSAPYAPSAPEAHGHEHSHETASPYFSEHHPPQPYGSN 60

Query: 61  YGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 120
           YGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI
Sbjct: 61  YGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 120

Query: 121 FLFRNPVDSSRMGPNEFTALWNCLGQWRNSFRPSRLKNSHFQGTFERYDRDRSGKIDALE 180
           FLFRNPVDSSRMGPNEFTALWNCLGQWR              GTFERYDRDRSGKIDALE
Sbjct: 121 FLFRNPVDSSRMGPNEFTALWNCLGQWR--------------GTFERYDRDRSGKIDALE 180

Query: 181 MRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHY 240
           MRDALYGLGYAVPSSVL+LLISLYDD+SGRRVEFNF SFVECGMIVKGLTEKFKEKDRHY
Sbjct: 181 MRDALYGLGYAVPSSVLKLLISLYDDQSGRRVEFNFGSFVECGMIVKGLTEKFKEKDRHY 240

Query: 241 TGSATLTYEDFMSTILPFLVSYS 263
           TGSA LTYEDFMSTILPFLVSYS
Sbjct: 241 TGSAMLTYEDFMSTILPFLVSYS 249

[Close]

BLAST of Cp4.1LG20g06900 vs. ExPASy TrEMBL
Match: A0A6J1HR40 (probable calcium-binding protein CML48 OS=Cucurbita maxima OX=3661 GN=LOC111466605 PE=4 SV=1)

HSP 1 Score: 447 bits (1151), Expect = 8.28e-158
Identity = 224/263 (85.17%), Postives = 239/263 (90.87%), Query Frame = 0


Query: 1   MASFFGRFTSHSSSSSNSKPHAPSAPYAPSAPEAHGHEHSHGTASPYFSENHPPQPYGSN 60
           MASFFGRFTS+S+S+S+S  HAPSAP APS PE +GH +SHGTASPY+S+ H PQPYGSN
Sbjct: 1   MASFFGRFTSYSNSNSHSHSHAPSAPSAPSEPETYGHGNSHGTASPYYSQQHQPQPYGSN 60

Query: 61  YGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLI 120
           YGGVSSYGSYGFPPGT PEVIRSFQMVD+D SGFIDENELQ+ALSSGYQRFSLRTVRLLI
Sbjct: 61  YGGVSSYGSYGFPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLRTVRLLI 120

Query: 121 FLFRNPVDSSRMGPNEFTALWNCLGQWRNSFRPSRLKNSHFQGTFERYDRDRSGKIDALE 180
           FLFRNP+DSSRMGPNEFTALWNCLGQWR              GTFERYDRDRSG+IDALE
Sbjct: 121 FLFRNPIDSSRMGPNEFTALWNCLGQWR--------------GTFERYDRDRSGRIDALE 180

Query: 181 MRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHY 240
           MRDALYGLGYAVPSSVL+LLISLYDDRSGRRVE+NFDSFVECGMIVKGLTEKFKEKD+HY
Sbjct: 181 MRDALYGLGYAVPSSVLQLLISLYDDRSGRRVEYNFDSFVECGMIVKGLTEKFKEKDKHY 240

Query: 241 TGSATLTYEDFMSTILPFLVSYS 263
           TGSATLTYEDFMSTILPFLVSYS
Sbjct: 241 TGSATLTYEDFMSTILPFLVSYS 249

[Close]

BLAST of Cp4.1LG20g06900 vs. ExPASy TrEMBL
Match: A0A5D3E617 (Putative calcium-binding protein CML48 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G003180 PE=4 SV=1)

HSP 1 Score: 442 bits (1138), Expect = 9.16e-156
Identity = 227/267 (85.02%), Postives = 237/267 (88.76%), Query Frame = 0


Query: 1   MASFFGRFTSHSSSSSNSKP----HAPSAPYAPSAPEAHGHEHSHGTASPYFSENHPPQP 60
           MASFF RFTS+S+SSSNS      HAPSAP APSAPE  GHE SH TASPY+SE H PQP
Sbjct: 1   MASFFNRFTSYSNSSSNSNSNSNSHAPSAPSAPSAPETQGHELSHATASPYYSEQHQPQP 60

Query: 61  YGSNYGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120
           YGSNYGGVSSYGSYGFPPGT PEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV
Sbjct: 61  YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120

Query: 121 RLLIFLFRNPVDSSRMGPNEFTALWNCLGQWRNSFRPSRLKNSHFQGTFERYDRDRSGKI 180
           RLLIFLFRNP+DSSRMGPNEFTALWNCLGQWR              G FERYDRDRSG+I
Sbjct: 121 RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWR--------------GMFERYDRDRSGRI 180

Query: 181 DALEMRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEK 240
           DALEMRDALYGLGYAVPSSVL+LLISLYDDRSG++VEFNFDSFVECGMIVKGLTEKFKEK
Sbjct: 181 DALEMRDALYGLGYAVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEK 240

Query: 241 DRHYTGSATLTYEDFMSTILPFLVSYS 263
           DR+YTGSATLTYEDFMSTILPFLVSY+
Sbjct: 241 DRNYTGSATLTYEDFMSTILPFLVSYN 253

[Close]

BLAST of Cp4.1LG20g06900 vs. ExPASy TrEMBL
Match: A0A1S3CFY8 (probable calcium-binding protein CML48 OS=Cucumis melo OX=3656 GN=LOC103500001 PE=4 SV=1)

HSP 1 Score: 442 bits (1138), Expect = 9.16e-156
Identity = 227/267 (85.02%), Postives = 237/267 (88.76%), Query Frame = 0


Query: 1   MASFFGRFTSHSSSSSNSKP----HAPSAPYAPSAPEAHGHEHSHGTASPYFSENHPPQP 60
           MASFF RFTS+S+SSSNS      HAPSAP APSAPE  GHE SH TASPY+SE H PQP
Sbjct: 1   MASFFNRFTSYSNSSSNSNSNSNSHAPSAPSAPSAPETQGHELSHATASPYYSEQHQPQP 60

Query: 61  YGSNYGGVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120
           YGSNYGGVSSYGSYGFPPGT PEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV
Sbjct: 61  YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120

Query: 121 RLLIFLFRNPVDSSRMGPNEFTALWNCLGQWRNSFRPSRLKNSHFQGTFERYDRDRSGKI 180
           RLLIFLFRNP+DSSRMGPNEFTALWNCLGQWR              G FERYDRDRSG+I
Sbjct: 121 RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWR--------------GMFERYDRDRSGRI 180

Query: 181 DALEMRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEK 240
           DALEMRDALYGLGYAVPSSVL+LLISLYDDRSG++VEFNFDSFVECGMIVKGLTEKFKEK
Sbjct: 181 DALEMRDALYGLGYAVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEK 240

Query: 241 DRHYTGSATLTYEDFMSTILPFLVSYS 263
           DR+YTGSATLTYEDFMSTILPFLVSY+
Sbjct: 241 DRNYTGSATLTYEDFMSTILPFLVSYN 253

[Close]

BLAST of Cp4.1LG20g06900 vs. TAIR 10
Match: AT2G27480.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 213.8 bits (543), Expect = 1.7e-55
Identity = 118/251 (47.01%), Postives = 163/251 (64.94%), Query Frame = 0


Query: 16  SNSKPHAPSAPYAPSA--PEAHGHEHSHGTASPYFSENHPPQPYGSNYGGVSSYGSYGFP 75
           S S  +APSAP  P +   + H  E  +  A P    ++ P    S+Y G+       F 
Sbjct: 2   SYSNAYAPSAPELPESFVQQQHDGESRYTYAYP----SYQPTQQFSSYSGM-------FS 61

Query: 76  PGTPPEVIRSFQMVDRDGSGFIDENELQQALS-SGYQRFSLRTVRLLIFLFRNPVDS-SR 135
           P T PE++RSF+  DR+ SGF++E+EL+QALS SGY   S RT+RLL+F+++ PVDS  R
Sbjct: 62  PETHPEIVRSFESADRNRSGFLEESELRQALSLSGYDGISNRTIRLLLFIYKIPVDSLLR 121

Query: 136 MGPNEFTALWNCLGQWRNSFRPSRLKNSHFQGTFERYDRDRSGKIDALEMRDALYGLGYA 195
           +GP E+  LWNCL QWR                F RYDRDRSGK+++ ++RDA Y LG  
Sbjct: 122 LGPKEYVELWNCLAQWR--------------AIFNRYDRDRSGKMNSTQLRDAFYNLGCV 181

Query: 196 VPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDF 255
           +P+SV +L++S +DD +G+ V+  FDSF+ECGMIVKGLTEKF+E D  YTG ATL+Y+ F
Sbjct: 182 LPTSVHQLIVSQFDDGTGKTVDLCFDSFLECGMIVKGLTEKFRENDPGYTGYATLSYDVF 227

Query: 256 MSTILPFLVSY 263
           M  ++PF+ +Y
Sbjct: 242 MLMVIPFIATY 227

[Close]

BLAST of Cp4.1LG20g06900 vs. TAIR 10
Match: AT5G04170.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 205.3 bits (521), Expect = 6.0e-53
Identity = 118/251 (47.01%), Postives = 153/251 (60.96%), Query Frame = 0


Query: 29  PSAPEAHGHEHS----HGTASPYFSENHPPQP----YGSNYGGV---SSYGS-------Y 88
           PS P  HG  +     HG +        PP+P    +G  YGG    +SYGS        
Sbjct: 119 PSQPSGHGGGYGATPPHGVSDYGSYGGAPPRPASSGHGGGYGGYPPQASYGSPFASLIPS 178

Query: 89  GFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLIFLFRNPVDSS 148
           GF PGT P ++  FQ  D+DGSGFID+ ELQ ALSS  QRFS+RTV LL++LF N  ++ 
Sbjct: 179 GFAPGTDPNIVACFQAADQDGSGFIDDKELQGALSSYQQRFSMRTVHLLMYLFTNS-NAM 238

Query: 149 RMGPNEFTALWNCLGQWRNSFRPSRLKNSHFQGTFERYDRDRSGKIDALEMRDALYGLGY 208
           ++GP EFTAL+  L  WR+               FER D+DRSG+ID  E+RDAL  LG+
Sbjct: 239 KIGPKEFTALFYSLQNWRS--------------IFERSDKDRSGRIDVNELRDALLSLGF 298

Query: 209 AVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYED 262
           +V   VL+LL+S +D   G+     +D+F+EC + VKGLTEKFKEKD  Y+GSAT  YE 
Sbjct: 299 SVSPVVLDLLVSKFDKSGGKNRAIEYDNFIECCLTVKGLTEKFKEKDTAYSGSATFNYES 354

[Close]

BLAST of Cp4.1LG20g06900 vs. TAIR 10
Match: AT3G10300.3 (Calcium-binding EF-hand family protein )

HSP 1 Score: 191.0 bits (484), Expect = 1.2e-48
Identity = 118/268 (44.03%), Postives = 152/268 (56.72%), Query Frame = 0


Query: 19  KPHAPSAPYAPSAPEAHGHEHSHGTASPYFSENHPPQP--YGSNYGGV------------ 78
           KPH   A Y   +P  +G   S G  S Y      PQ   +G  YGG             
Sbjct: 86  KPH--GASYGSPSPGGYGAHPSSG-PSDYGGYGGAPQQSGHGGGYGGAPQQSGHGGGYGA 145

Query: 79  ----SSYGS-------YGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSL 138
               +SYGS         FPPGT P ++  FQ  DRD SGFID+ ELQ ALSS  Q FS+
Sbjct: 146 PPPQASYGSPFASLVPSAFPPGTDPNIVACFQAADRDNSGFIDDKELQGALSSYNQSFSI 205

Query: 139 RTVRLLIFLFRNPVDSSRMGPNEFTALWNCLGQWRNSFRPSRLKNSHFQGTFERYDRDRS 198
           RTV LL++LF N  +  ++GP EFT+L+  L  WR+               FER+D+DRS
Sbjct: 206 RTVHLLMYLFTNS-NVRKIGPKEFTSLFFSLQNWRS--------------IFERFDKDRS 265

Query: 199 GKIDALEMRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKF 258
           G+ID  E+RDAL  LG++V   +L+LL+S +D   GR     +D+F+EC + VKGLTEKF
Sbjct: 266 GRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIECCLTVKGLTEKF 325

Query: 259 KEKDRHYTGSATLTYEDFMSTILPFLVS 262
           KEKD   +GSA   YE+FM T+LPFLV+
Sbjct: 326 KEKDTALSGSAIFNYENFMLTVLPFLVA 335

[Close]

BLAST of Cp4.1LG20g06900 vs. TAIR 10
Match: AT3G10300.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 139.0 bits (349), Expect = 5.3e-33
Identity = 92/228 (40.35%), Postives = 121/228 (53.07%), Query Frame = 0


Query: 19  KPHAPSAPYAPSAPEAHGHEHSHGTASPYFSENHPPQP--YGSNYGGV------------ 78
           KPH   A Y   +P  +G   S G  S Y      PQ   +G  YGG             
Sbjct: 86  KPH--GASYGSPSPGGYGAHPSSG-PSDYGGYGGAPQQSGHGGGYGGAPQQSGHGGGYGA 145

Query: 79  ----SSYGS-------YGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSL 138
               +SYGS         FPPGT P ++  FQ  DRD SGFID+ ELQ ALSS  Q FS+
Sbjct: 146 PPPQASYGSPFASLVPSAFPPGTDPNIVACFQAADRDNSGFIDDKELQGALSSYNQSFSI 205

Query: 139 RTVRLLIFLFRNPVDSSRMGPNEFTALWNCLGQWRNSFRPSRLKNSHFQGTFERYDRDRS 198
           RTV LL++LF N  +  ++GP EFT+L+  L  WR+               FER+D+DRS
Sbjct: 206 RTVHLLMYLFTNS-NVRKIGPKEFTSLFFSLQNWRS--------------IFERFDKDRS 265

Query: 199 GKIDALEMRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVE 222
           G+ID  E+RDAL  LG++V   +L+LL+S +D   GR     +D+F+E
Sbjct: 266 GRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIE 295

[Close]

BLAST of Cp4.1LG20g06900 vs. TAIR 10
Match: AT3G10300.4 (Calcium-binding EF-hand family protein )

HSP 1 Score: 139.0 bits (349), Expect = 5.3e-33
Identity = 92/228 (40.35%), Postives = 121/228 (53.07%), Query Frame = 0


Query: 19  KPHAPSAPYAPSAPEAHGHEHSHGTASPYFSENHPPQP--YGSNYGGV------------ 78
           KPH   A Y   +P  +G   S G  S Y      PQ   +G  YGG             
Sbjct: 86  KPH--GASYGSPSPGGYGAHPSSG-PSDYGGYGGAPQQSGHGGGYGGAPQQSGHGGGYGA 145

Query: 79  ----SSYGS-------YGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSL 138
               +SYGS         FPPGT P ++  FQ  DRD SGFID+ ELQ ALSS  Q FS+
Sbjct: 146 PPPQASYGSPFASLVPSAFPPGTDPNIVACFQAADRDNSGFIDDKELQGALSSYNQSFSI 205

Query: 139 RTVRLLIFLFRNPVDSSRMGPNEFTALWNCLGQWRNSFRPSRLKNSHFQGTFERYDRDRS 198
           RTV LL++LF N  +  ++GP EFT+L+  L  WR+               FER+D+DRS
Sbjct: 206 RTVHLLMYLFTNS-NVRKIGPKEFTSLFFSLQNWRS--------------IFERFDKDRS 265

Query: 199 GKIDALEMRDALYGLGYAVPSSVLELLISLYDDRSGRRVEFNFDSFVE 222
           G+ID  E+RDAL  LG++V   +L+LL+S +D   GR     +D+F+E
Sbjct: 266 GRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIE 295

The following BLAST results are available for this feature:

BLAST of Cp4.1LG20g06900 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZQH1	2.4e-54	47.01	Probable calcium-binding protein CML48 OS=Arabidopsis thaliana OX=3702 GN=CML48 ...	[more]
Q2QY10	1.0e-52	45.98	Calcium-binding protein CBP OS=Oryza sativa subsp. japonica OX=39947 GN=CBP PE=2...	[more]
Q9FYE4	8.5e-52	47.01	Probable calcium-binding protein CML50 OS=Arabidopsis thaliana OX=3702 GN=CML50 ...	[more]
Q8W4L0	1.7e-47	44.03	Probable calcium-binding protein CML49 OS=Arabidopsis thaliana OX=3702 GN=CML49 ...	[more]
Q5PQ53	1.0e-25	33.48	Peflin OS=Xenopus laevis OX=8355 GN=pef1 PE=1 SV=1	[more]

BLAST of Cp4.1LG20g06900 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023519854.1	2.16e-176	94.68	probable calcium-binding protein CML48 [Cucurbita pepo subsp. pepo]	[more]
KAG6583656.1	5.08e-175	93.92	putative calcium-binding protein CML48, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022932202.1	4.17e-174	93.54	probable calcium-binding protein CML48 [Cucurbita moschata]	[more]
XP_022970340.1	3.15e-169	91.63	probable calcium-binding protein CML48 [Cucurbita maxima]	[more]
XP_022967101.1	1.71e-157	85.17	probable calcium-binding protein CML48 [Cucurbita maxima]	[more]

BLAST of Cp4.1LG20g06900 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EVZ6	2.02e-174	93.54	probable calcium-binding protein CML48 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A6J1I2L1	1.52e-169	91.63	probable calcium-binding protein CML48 OS=Cucurbita maxima OX=3661 GN=LOC1114693...	[more]
A0A6J1HR40	8.28e-158	85.17	probable calcium-binding protein CML48 OS=Cucurbita maxima OX=3661 GN=LOC1114666...	[more]
A0A5D3E617	9.16e-156	85.02	Putative calcium-binding protein CML48 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CFY8	9.16e-156	85.02	probable calcium-binding protein CML48 OS=Cucumis melo OX=3656 GN=LOC103500001 P...	[more]

BLAST of Cp4.1LG20g06900 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G27480.1	1.7e-55	47.01	Calcium-binding EF-hand family protein	[more]
AT5G04170.1	6.0e-53	47.01	Calcium-binding EF-hand family protein	[more]
AT3G10300.3	1.2e-48	44.03	Calcium-binding EF-hand family protein	[more]
AT3G10300.2	5.3e-33	40.35	Calcium-binding EF-hand family protein	[more]
AT3G10300.4	5.3e-33	40.35	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 160..188 e-value: 0.22 score: 20.6 coord: 79..107 e-value: 1.9E-4 score: 30.8
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 164..182 e-value: 0.002 score: 17.6
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 80..106 e-value: 2.0E-6 score: 27.0
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 156..191 score: 12.253878
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 75..110 score: 12.05861
None	No IPR available	GENE3D	1.10.238.10		coord: 70..260 e-value: 4.9E-34 score: 119.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..60
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..23
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 44..60
None	No IPR available	PANTHER	PTHR46824:SF2	CALCIUM-BINDING PROTEIN CML48-RELATED	coord: 18..261
None	No IPR available	CDD	cd16180	EFh_PEF_Group_I	coord: 79..258 e-value: 1.07623E-68 score: 207.766
IPR044590	Probable calcium-binding protein CML48/49/50	PANTHER	PTHR46824	CALCIUM-BINDING PROTEIN CML48-RELATED	coord: 18..261
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 88..100
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 169..181
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 71..255

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG20g06900.1	Cp4.1LG20g06900.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding