Cp4.1LG20g04750 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG20g04750
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionheat stress transcription factor A-4c-like
LocationCp4.1LG20: 2777680 .. 2779992 (-)
RNA-Seq ExpressionCp4.1LG20g04750
SyntenyCp4.1LG20g04750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCTTTTTCCGCCATTTTAGTGACTGATAATTTTCTTCAAGCCCGTGTTAGGCAGTGCATCAATCAAAGATTTCGGTGTTTCCATGTAGGTTTCTCCCAATCAAAGCGAAGGAAAAGAGGTACAGGTGCCCTCAGAGTTTGGATTTTTAATCGTTCGTCTCTTTTCCCCCCTCCTCTTTTTGTTTGTTGAATCTAAGGGCATTTTTTAATCGTCTTTCGTTTCCACTTTTTTGTTATTTCAATTGAATTCATTGGATTTCATTAAGTATTTTATTACTTCAGTTCAAACCGTTAAATTCCGTTCCGCAACTCTCTGTTCTTTTGTCTTCTTTAGGCTATGTTGAGCAAGTTCTACTCGAGTTGACTGGAGAAGGGTCAATGCAAAATTCGTAATTTCTTTCAAAATCCCATATGGAAATCGATAATTCTGAATTGGGTTTGCCTGGAAATCAAGCTTCGATGGAAATTTCTGGGATTCACCAGTGTTTTTGTGATTTAAATGAAGACCCAACTGGGGGTTTTTGCTTTTGAGGTTTGAAGTTCTTTGGTGGATTATCGTTGTTGTTCTTGAAGTTTGATGTGGTGAATCTGTTGAGAGTTGTATTGATATTTTTGTAAGGATTTTGATACGCGATCTTCTGTTTGCAATTTGGTGGATTGGGAGTTGTTAATGTACAGCTTTTGGTTTCTTAATTTGGGGGTTCTTATTTTGGGGATTGGAGAGGTTAGGATATAGAATGGATGAAGCTCAGGGAAGCGGCTTGACTTCGTTGCCTCCATTTTTAGTCAAAACATATGATATGGTCGATGATCCTTCAACCAATTCGATTGTTTCATGGAGTTCAAGTGATAAAAGCTTTGTTGTTTGGAAACCACTGGAGTTCTCATCAGTTTTGTTGCCTAAATTCTTTAAGCATAGCAACTTCTCGAGCTTCATCAGGCAGCTCAATACTTATGTAAGATGATAAGTTTTTCATTTACTTGCTTATTGTTGTGATTGTGTCCTTGTTTTTGTTTTCTGAACATTTTGTTCATTACTTTAGGGGTTCAAGAAGGTGGATCCTGATCAATGGGAATTTGCCAATGATGATTTTGTTAGAGGTGAGCAACACCTGATGAAGAACATCCACAGGAGAAAACCAGTTCATAGTCATTCTTTACAGAACCCCCATGGACAAGGAGTATCTCCTCCGCTAACTGAAGTCGAGAGAAAGAGCTTCGAGGATAACATCGAGACGCTGAAACGGGATAAAGAGCAGCTTGTTCTTGAGTTGCGGAAACACAAACAAGAGTATCAAGGAGTTGTGTTGCAAATGCAGAATTTGAAAGATCGCTTTCAATGTGTTCAACAAGGCATGCAATTATTTATCAGTCTGATCGCTCGTTTTTTGCATAAACCAGGACTTCGCTTGGATCTTCTGCCACAATTGGAAACTTCTGATAGAAAGAGGAGATTGCCTAGAGTTTCTTACAACAATAGTGAAGATAACCTTGAGGATAATCAGATGGGGACAACTCAAACCATTAGTAGAGAAAATATGGATTGTAGTTTTGATCCAATTTTGAAAGAAGAACAGTTTGAACTTCTTGAGACATCCATAGCCTTTTGGGAAGGCATTATCCATAGTTATGGTCAAACAATTAGTCCACTTGATTCCAGCTCAAACCTGGAGTTGGGTGGATCTGTAAGTCATGCCAGTAGCCCTGCTGTAACTTGCAGGCAAGTTAGTGAGGAGCTTCGGTGTAAATCACCAGGAATTGACATGAATTTGGAGCCCATGGCAACCGTTGCTCCTGAATCTGTAGCCTCGAAAGATCAGGCAGCTGGAGTCAAAGCTCCAGTACCAACTGGTGTCAATGATGTTTTCTGGCAGCAATTCTTGACGGAGAATCCTGGTTCATCTGACCCACAAGAAGTTCAATCAGCCAGAAAAGATTCTGATGTCATAATCGAAGAAAACAGACCGAGCGATCAAGGAAATTTTTGGTGGAACACGAGGAGTGTAAATAATGTTGTAGAACAGATAGGGAACCTCGCTCCAGCAGAGAAATTTTCATAGTAGGTTGATTGTTTTTCTGAACTACTGATTTTCCTTAGCTCACATGTACATAATTTATTATAAAATGGAACATTTGGGCTCTCATCTATGTCTTTCTTCACATGTTTTCATTGGTTTTGATTCCCTCATAATGCTTGACTTGTTTAGAGAATTAGTGGCTGTACCTGTGAATGAATAATAATTTTGACTACTTTGGTTGTGTAATAGGGAATAGGCATTAGTGTTGGATCATTGTATAGTATGCAAGTGATTA

mRNA sequence

ATCTTTTTCCGCCATTTTAGTGACTGATAATTTTCTTCAAGCCCGTGTTAGGCAGTGCATCAATCAAAGATTTCGGTGTTTCCATGTAGGTTTCTCCCAATCAAAGCGAAGGAAAAGAGGCTATGTTGAGCAAGTTCTACTCGAGTTGACTGGAGAAGGGTCAATGCAAAATTCGTAATTTCTTTCAAAATCCCATATGGAAATCGATAATTCTGAATTGGGTTTGCCTGGAAATCAAGCTTCGATGGAAATTTCTGGGATTCACCAGTGTTTTTGTGATTTAAATGAAGACCCAACTGGGGGTTTTTGCTTTTGAGGTTTGAAGTTCTTTGGTGGATTATCGTTGTTGTTCTTGAAGTTTGATGTGGTGAATCTGTTGAGAGTTGTATTGATATTTTTGTAAGGATTTTGATACGCGATCTTCTGTTTGCAATTTGGTGGATTGGGAGTTGTTAATGTACAGCTTTTGGTTTCTTAATTTGGGGGTTCTTATTTTGGGGATTGGAGAGGTTAGGATATAGAATGGATGAAGCTCAGGGAAGCGGCTTGACTTCGTTGCCTCCATTTTTAGTCAAAACATATGATATGGTCGATGATCCTTCAACCAATTCGATTGTTTCATGGAGTTCAAGTGATAAAAGCTTTGTTGTTTGGAAACCACTGGAGTTCTCATCAGTTTTGTTGCCTAAATTCTTTAAGCATAGCAACTTCTCGAGCTTCATCAGGCAGCTCAATACTTATGGGTTCAAGAAGGTGGATCCTGATCAATGGGAATTTGCCAATGATGATTTTGTTAGAGGTGAGCAACACCTGATGAAGAACATCCACAGGAGAAAACCAGTTCATAGTCATTCTTTACAGAACCCCCATGGACAAGGAGTATCTCCTCCGCTAACTGAAGTCGAGAGAAAGAGCTTCGAGGATAACATCGAGACGCTGAAACGGGATAAAGAGCAGCTTGTTCTTGAGTTGCGGAAACACAAACAAGAGTATCAAGGAGTTGTGTTGCAAATGCAGAATTTGAAAGATCGCTTTCAATGTGTTCAACAAGGCATGCAATTATTTATCAGTCTGATCGCTCGTTTTTTGCATAAACCAGGACTTCGCTTGGATCTTCTGCCACAATTGGAAACTTCTGATAGAAAGAGGAGATTGCCTAGAGTTTCTTACAACAATAGTGAAGATAACCTTGAGGATAATCAGATGGGGACAACTCAAACCATTAGTAGAGAAAATATGGATTGTAGTTTTGATCCAATTTTGAAAGAAGAACAGTTTGAACTTCTTGAGACATCCATAGCCTTTTGGGAAGGCATTATCCATAGTTATGGTCAAACAATTAGTCCACTTGATTCCAGCTCAAACCTGGAGTTGGGTGGATCTGTAAGTCATGCCAGTAGCCCTGCTGTAACTTGCAGGCAAGTTAGTGAGGAGCTTCGGTGTAAATCACCAGGAATTGACATGAATTTGGAGCCCATGGCAACCGTTGCTCCTGAATCTGTAGCCTCGAAAGATCAGGCAGCTGGAGTCAAAGCTCCAGTACCAACTGGTGTCAATGATGTTTTCTGGCAGCAATTCTTGACGGAGAATCCTGGTTCATCTGACCCACAAGAAGTTCAATCAGCCAGAAAAGATTCTGATGTCATAATCGAAGAAAACAGACCGAGCGATCAAGGAAATTTTTGGTGGAACACGAGGAGTGTAAATAATGTTGTAGAACAGATAGGGAACCTCGCTCCAGCAGAGAAATTTTCATAGTAGGTTGATTGTTTTTCTGAACTACTGATTTTCCTTAGCTCACATGTACATAATTTATTATAAAATGGAACATTTGGGCTCTCATCTATGTCTTTCTTCACATGTTTTCATTGGTTTTGATTCCCTCATAATGCTTGACTTGTTTAGAGAATTAGTGGCTGTACCTGTGAATGAATAATAATTTTGACTACTTTGGTTGTGTAATAGGGAATAGGCATTAGTGTTGGATCATTGTATAGTATGCAAGTGATTA

Coding sequence (CDS)

ATGGATGAAGCTCAGGGAAGCGGCTTGACTTCGTTGCCTCCATTTTTAGTCAAAACATATGATATGGTCGATGATCCTTCAACCAATTCGATTGTTTCATGGAGTTCAAGTGATAAAAGCTTTGTTGTTTGGAAACCACTGGAGTTCTCATCAGTTTTGTTGCCTAAATTCTTTAAGCATAGCAACTTCTCGAGCTTCATCAGGCAGCTCAATACTTATGGGTTCAAGAAGGTGGATCCTGATCAATGGGAATTTGCCAATGATGATTTTGTTAGAGGTGAGCAACACCTGATGAAGAACATCCACAGGAGAAAACCAGTTCATAGTCATTCTTTACAGAACCCCCATGGACAAGGAGTATCTCCTCCGCTAACTGAAGTCGAGAGAAAGAGCTTCGAGGATAACATCGAGACGCTGAAACGGGATAAAGAGCAGCTTGTTCTTGAGTTGCGGAAACACAAACAAGAGTATCAAGGAGTTGTGTTGCAAATGCAGAATTTGAAAGATCGCTTTCAATGTGTTCAACAAGGCATGCAATTATTTATCAGTCTGATCGCTCGTTTTTTGCATAAACCAGGACTTCGCTTGGATCTTCTGCCACAATTGGAAACTTCTGATAGAAAGAGGAGATTGCCTAGAGTTTCTTACAACAATAGTGAAGATAACCTTGAGGATAATCAGATGGGGACAACTCAAACCATTAGTAGAGAAAATATGGATTGTAGTTTTGATCCAATTTTGAAAGAAGAACAGTTTGAACTTCTTGAGACATCCATAGCCTTTTGGGAAGGCATTATCCATAGTTATGGTCAAACAATTAGTCCACTTGATTCCAGCTCAAACCTGGAGTTGGGTGGATCTGTAAGTCATGCCAGTAGCCCTGCTGTAACTTGCAGGCAAGTTAGTGAGGAGCTTCGGTGTAAATCACCAGGAATTGACATGAATTTGGAGCCCATGGCAACCGTTGCTCCTGAATCTGTAGCCTCGAAAGATCAGGCAGCTGGAGTCAAAGCTCCAGTACCAACTGGTGTCAATGATGTTTTCTGGCAGCAATTCTTGACGGAGAATCCTGGTTCATCTGACCCACAAGAAGTTCAATCAGCCAGAAAAGATTCTGATGTCATAATCGAAGAAAACAGACCGAGCGATCAAGGAAATTTTTGGTGGAACACGAGGAGTGTAAATAATGTTGTAGAACAGATAGGGAACCTCGCTCCAGCAGAGAAATTTTCATAG

Protein sequence

MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKHSNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGVSPPLTEVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQLFISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENMDCSFDPILKEEQFELLETSIAFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQVSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSSDPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKFS
Homology
BLAST of Cp4.1LG20g04750 vs. ExPASy Swiss-Prot
Match: O49403 (Heat stress transcription factor A-4a OS=Arabidopsis thaliana OX=3702 GN=HSFA4A PE=1 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 6.9e-93
Identity = 195/415 (46.99%), Postives = 265/415 (63.86%), Query Frame = 0

Query: 1   MDE-AQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFK 60
           MDE   G   +SLPPFL KTY+MVDD S++SIVSWS S+KSF+VW P EFS  LLP+FFK
Sbjct: 1   MDENNHGVSSSSLPPFLTKTYEMVDDSSSDSIVSWSQSNKSFIVWNPPEFSRDLLPRFFK 60

Query: 61  HSNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQG 120
           H+NFSSFIRQLNTYGF+K DP+QWEFANDDFVRG+ HLMKNIHRRKPVHSHSL  P+ Q 
Sbjct: 61  HNNFSSFIRQLNTYGFRKADPEQWEFANDDFVRGQPHLMKNIHRRKPVHSHSL--PNLQA 120

Query: 121 VSPPLTEVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQ 180
              PLT+ ER    + IE L ++KE L+ EL K  +E +   +Q++ LK+R Q +++  +
Sbjct: 121 QLNPLTDSERVRMNNQIERLTKEKEGLLEELHKQDEEREVFEMQVKELKERLQHMEKRQK 180

Query: 181 LFISLIARFLHKPGLRLDLLPQL-ETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISREN 240
             +S +++ L KPGL L+L P + ET++RKRR PR+ +   E  LE+N+   T  + RE 
Sbjct: 181 TMVSFVSQVLEKPGLALNLSPCVPETNERKRRFPRIEFFPDEPMLEENK---TCVVVREE 240

Query: 241 MDCSFDPILKEEQFELLETSIAFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTC 300
              S     +E Q E LE+SIA WE ++    +++    S   L++  S +   SP ++C
Sbjct: 241 GSTSPSSHTREHQVEQLESSIAIWENLVSDSCESMLQSRSMMTLDVDESSTFPESPPLSC 300

Query: 301 RQVSEELRCKSPG----IDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLT 360
            Q+S + R KSP     IDMN EP  +    +VA+         P   G ND FWQQF +
Sbjct: 301 IQLSVDSRLKSPPSPRIIDMNCEPDGSKEQNTVAAP------PPPPVAGANDGFWQQFFS 360

Query: 361 ENPGSSDPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEK 410
           ENPGS++ +EVQ  RKD     ++         WWN+R+VN + EQ+G+L  +E+
Sbjct: 361 ENPGSTEQREVQLERKDD----KDKAGVRTEKCWWNSRNVNAITEQLGHLTSSER 400

BLAST of Cp4.1LG20g04750 vs. ExPASy Swiss-Prot
Match: Q9FK72 (Heat stress transcription factor A-4c OS=Arabidopsis thaliana OX=3702 GN=HSFA4C PE=2 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 1.8e-80
Identity = 182/401 (45.39%), Postives = 237/401 (59.10%), Query Frame = 0

Query: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60
           MDE  G G +SLPPFL KTY+MVDD S++S+V+WS ++KSF+V  P EFS  LLP+FFKH
Sbjct: 1   MDENNG-GSSSLPPFLTKTYEMVDDSSSDSVVAWSENNKSFIVKNPAEFSRDLLPRFFKH 60

Query: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120
            NFSSFIRQLNTYGF+KVDP++WEF NDDFVRG  +LMKNIHRRKPVHSHSL N   Q  
Sbjct: 61  KNFSSFIRQLNTYGFRKVDPEKWEFLNDDFVRGRPYLMKNIHRRKPVHSHSLVNLQAQN- 120

Query: 121 SPPLTEVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQL 180
             PLTE ER+S ED IE LK +KE L+ EL+  +QE +   LQ+  LKDR Q ++Q  + 
Sbjct: 121 --PLTESERRSMEDQIERLKNEKEGLLAELQNQEQERKEFELQVTTLKDRLQHMEQHQKS 180

Query: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENMD 240
            ++ +++ L KPGL L+    LE  +R++R             ++N +  + +       
Sbjct: 181 IVAYVSQVLGKPGLSLN----LENHERRKR-----------RFQENSLPPSSS------- 240

Query: 241 CSFDPILKEEQFELLETSIAFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300
                    EQ E LE+S+ FWE ++           S S  + G   S     A     
Sbjct: 241 -------HIEQVEKLESSLTFWENLV-----------SESCEKSGLQSSSMDHDAAESSL 300

Query: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVP-TGVNDVFWQQFLTENPGS 360
              + R KS  IDMN EP  TV               AP P TGVND FW+Q LTENPGS
Sbjct: 301 SIGDTRPKSSKIDMNSEPPVTVT--------------APAPKTGVNDDFWEQCLTENPGS 343

Query: 361 SDPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQ 401
           ++ QEVQS R+D       N+  +Q  +WWN+ +VNN+ E+
Sbjct: 361 TEQQEVQSERRDVGNDNNGNKIGNQRTYWWNSGNVNNITEK 343

BLAST of Cp4.1LG20g04750 vs. ExPASy Swiss-Prot
Match: Q94J16 (Heat stress transcription factor A-4b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA4B PE=2 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 5.5e-74
Identity = 176/443 (39.73%), Postives = 244/443 (55.08%), Query Frame = 0

Query: 6   GSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKHSNFSS 65
           G G  SLPPFL KTY+MVDDPST+++V W+ +  SFVV    EF   LLPK+FKH+NFSS
Sbjct: 4   GGGGGSLPPFLSKTYEMVDDPSTDAVVGWTPAGTSFVVANQPEFCRDLLPKYFKHNNFSS 63

Query: 66  FIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGVSPPLT 125
           F+RQLNTYGF+KVDP+QWEFAN+DF++G++H +KNIHRRKP+ SHS    H QG   PLT
Sbjct: 64  FVRQLNTYGFRKVDPEQWEFANEDFIKGQRHRLKNIHRRKPIFSHS---SHSQGAG-PLT 123

Query: 126 EVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQLFISLI 185
           + ERK +E+ IE LK D   L  EL+ +  +   +  +MQ L+++   V+   +  IS +
Sbjct: 124 DNERKDYEEEIERLKSDNAALSSELQNNTLKKLNMEKRMQALEEKLFVVEDQQRSLISYV 183

Query: 186 ARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQM-------GTTQTISREN 245
              +  PG     + Q +   +KRRLP     + + N ++NQ+          QT  RE+
Sbjct: 184 REIVKAPGFLSSFVQQQDHHRKKRRLPIPISFHEDANTQENQIMPCDLTNSPAQTFYRES 243

Query: 246 MDCSFDPILKEEQF-----ELLETSIAFWEGI-----------IHSYGQT----ISPL-- 305
            D     +   E F     E     I++ +G+           +HS G++     SP   
Sbjct: 244 FDKMESSLNSLENFLREASEEFGNDISYDDGVPGPSSTVVLTELHSPGESDPRVSSPPTR 303

Query: 306 ---------DSSSNLELGGSVSHASSPAVTCRQVSEELRCKSPGIDMNLEPMATVAPESV 365
                    DS S+ ++  S S A SP +       + R K   ID+N EP  T   E+ 
Sbjct: 304 MRTSSAGAGDSHSSRDVAESTSCAESPPIPQMHSRVDTRAKVSEIDVNSEPAVT---ETG 363

Query: 366 ASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSSDP-QEVQSARKDSDVIIEENRPSDQGN 410
            S+DQ A     V  G ND FWQQFLTE PGSSD  QE QS R+D    ++E +  D+ +
Sbjct: 364 PSRDQPAEEPPAVTPGANDGFWQQFLTEQPGSSDAHQEAQSERRDGGNKVDEMKSGDRQH 423

BLAST of Cp4.1LG20g04750 vs. ExPASy Swiss-Prot
Match: Q93VB5 (Heat stress transcription factor A-4d OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA4D PE=1 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 4.3e-58
Identity = 164/456 (35.96%), Postives = 242/456 (53.07%), Query Frame = 0

Query: 6   GSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKHSNFSS 65
           G G    PPFL+KTY+MV+D +TN +VSW     SFVVW PL+FS  LLPK+FKH+NFSS
Sbjct: 12  GGGGGGPPPFLIKTYEMVEDAATNHVVSWGPGGASFVVWNPLDFSRDLLPKYFKHNNFSS 71

Query: 66  FIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGVSPPLT 125
           FIRQLNTYGF+K+DP++WEFAN+DF+RG  HL+KNIHRRKPVHSHSLQN     ++ PL 
Sbjct: 72  FIRQLNTYGFRKIDPERWEFANEDFIRGHTHLLKNIHRRKPVHSHSLQNQ----INGPLA 131

Query: 126 EVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQLFISLI 185
           E ER+  E+ I  LK +K  LV +L++  Q+   +  QMQ ++ R   ++Q  +  ++ +
Sbjct: 132 ESERRELEEEINRLKYEKSILVADLQRQNQQQYVINWQMQAMEGRLVAMEQRQKNIVASL 191

Query: 186 ARFLHKPGLRL-DLLPQLETSDRKRRLPRVS-YNNSEDNLEDNQMGTTQTISRENMDC-S 245
              L + G  +   L + +   +KRR+P++  + +     E+ ++   Q I  +      
Sbjct: 192 CEMLQRRGGAVSSSLLESDHFSKKRRVPKMDLFVDDCAAGEEQKVFQFQGIGTDAPAMPP 251

Query: 246 FDPILKEEQFELLETSIAFWEGII---------------HSYGQT------------ISP 305
             P+   E F+ +E S+   E +                H +G T             +P
Sbjct: 252 VLPVTNGEAFDRVELSLVSLEKLFQRANDACTAAEEMYSHGHGGTEPSTAICPEEMNTAP 311

Query: 306 LDSSSNLELGGSVSHASSPAV--TCRQVSEELRCKSPGIDMNLE-PMATVAPE-SVASKD 365
           +++  +L+L  S+ H SSP        +S EL  +SPG   + E PMA +  +  V    
Sbjct: 312 METGIDLQLPASL-HPSSPNTGNAHLHLSTEL-TESPGFVQSPELPMAEIREDIHVTRYP 371

Query: 366 QAAGVKAPVPTG-----------------VNDVFWQQFLTENPGSS-DPQEVQSARKDSD 410
             A V + + +                   NDVFW++FLTE P S  D  E Q + KD D
Sbjct: 372 TQADVNSEIASSTDTSQDGTSETEASHGPTNDVFWERFLTETPRSCLDESERQESPKD-D 431

BLAST of Cp4.1LG20g04750 vs. ExPASy Swiss-Prot
Match: Q84T61 (Heat stress transcription factor A-1 OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA1 PE=2 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 7.8e-52
Identity = 140/362 (38.67%), Postives = 200/362 (55.25%), Query Frame = 0

Query: 10  TSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKHSNFSSFIRQ 69
           T+ PPFL+KTY+MVDDP+T+++VSW   + SFVVW   EF+  LLPK+FKHSNFSSF+RQ
Sbjct: 33  TAPPPFLMKTYEMVDDPATDAVVSWGPGNNSFVVWNTPEFARDLLPKYFKHSNFSSFVRQ 92

Query: 70  LNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVH-SHSLQNPHGQGVS-PPLTEV 129
           LNTYGF+KVDPD+WEFAN+ F+RG++HL+K I+RRKP H ++ +Q P       P   EV
Sbjct: 93  LNTYGFRKVDPDRWEFANEGFLRGQKHLLKTINRRKPTHGNNQVQQPQLPAAPVPACVEV 152

Query: 130 ERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQLFISLIAR 189
            +   E+ IE LKRDK  L+ EL + +Q+ Q    Q+Q L  R Q ++Q  Q  +S +A+
Sbjct: 153 GKFGMEEEIEMLKRDKNVLMQELVRLRQQQQTTDHQLQTLGKRLQGMEQRQQQMMSFLAK 212

Query: 190 FLHKPGLRLDLLPQLE-------TSDRKRRLPRVSYN-NSEDNLEDNQMGTTQTISRENM 249
            +H PG     + Q E        S++KRRLP+   + +SE    D Q+   Q +  E  
Sbjct: 213 AMHSPGFLAQFVQQNENSRRRIVASNKKRRLPKQDGSLDSESASLDGQIVKYQPMINEAA 272

Query: 250 DCSFDPILK---EEQFELLETSIAFWEGIIHSYGQTISPLDSSSNLELGG---------- 309
                 ILK     +FE +  S  F   ++ +Y      LDSSS+    G          
Sbjct: 273 KAMLRKILKLDSSHRFESMGNSDNF---LLENYMPNGQGLDSSSSTRNSGVTLAEVPANS 332

Query: 310 ---SVSHASSPAVTCRQVSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTG 346
               V+ +S  +  C   + +++C  P +  N  P       +V S  +A    AP PT 
Sbjct: 333 GLPYVATSSGLSAICSTSTPQIQC--PVVLDNGIPKEVPNMSAVPSVPKAV---APGPTD 386

BLAST of Cp4.1LG20g04750 vs. NCBI nr
Match: XP_023519250.1 (heat stress transcription factor A-4a-like [Cucurbita pepo subsp. pepo] >XP_023519251.1 heat stress transcription factor A-4a-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 820 bits (2118), Expect = 1.14e-299
Identity = 411/411 (100.00%), Postives = 411/411 (100.00%), Query Frame = 0

Query: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60
           MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH
Sbjct: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120
           SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV
Sbjct: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120

Query: 121 SPPLTEVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQL 180
           SPPLTEVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQL
Sbjct: 121 SPPLTEVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQL 180

Query: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENMD 240
           FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENMD
Sbjct: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENMD 240

Query: 241 CSFDPILKEEQFELLETSIAFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300
           CSFDPILKEEQFELLETSIAFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ
Sbjct: 241 CSFDPILKEEQFELLETSIAFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300

Query: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360
           VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS
Sbjct: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360

Query: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKFS 411
           DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKFS
Sbjct: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKFS 411

BLAST of Cp4.1LG20g04750 vs. NCBI nr
Match: XP_022927503.1 (heat stress transcription factor A-4c-like [Cucurbita moschata] >XP_022927504.1 heat stress transcription factor A-4c-like [Cucurbita moschata] >KAG7019556.1 Heat stress transcription factor A-4a [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 811 bits (2096), Expect = 2.57e-296
Identity = 405/411 (98.54%), Postives = 409/411 (99.51%), Query Frame = 0

Query: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60
           MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH
Sbjct: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120
           SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV
Sbjct: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120

Query: 121 SPPLTEVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQL 180
           SPPLTEVERKSFEDNIETLKRDKEQL+LELRKH+QEYQGVVLQMQNLKDRFQCVQQGMQL
Sbjct: 121 SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180

Query: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENMD 240
           FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISREN D
Sbjct: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD 240

Query: 241 CSFDPILKEEQFELLETSIAFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300
           CSFDPILKEEQFEL+ETS+ FWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ
Sbjct: 241 CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300

Query: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360
           VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS
Sbjct: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360

Query: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKFS 411
           DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKFS
Sbjct: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKFS 411

BLAST of Cp4.1LG20g04750 vs. NCBI nr
Match: KAG6583940.1 (Heat stress transcription factor A-4a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 776 bits (2004), Expect = 1.98e-281
Identity = 387/393 (98.47%), Postives = 391/393 (99.49%), Query Frame = 0

Query: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60
           MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH
Sbjct: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120
           SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV
Sbjct: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120

Query: 121 SPPLTEVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQL 180
           SPPLTEVERKSFEDNIETLKRDKEQL+LELRKH+QEYQGVVLQMQNLKDRFQCVQQGMQL
Sbjct: 121 SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180

Query: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENMD 240
           FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISREN D
Sbjct: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD 240

Query: 241 CSFDPILKEEQFELLETSIAFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300
           CSFDPILKEEQFEL+ETS+ FWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ
Sbjct: 241 CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300

Query: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360
           VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS
Sbjct: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360

Query: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRS 393
           DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRS
Sbjct: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRS 393

BLAST of Cp4.1LG20g04750 vs. NCBI nr
Match: XP_023000762.1 (heat stress transcription factor A-4a-like [Cucurbita maxima] >XP_023000763.1 heat stress transcription factor A-4a-like [Cucurbita maxima])

HSP 1 Score: 749 bits (1933), Expect = 7.50e-272
Identity = 372/388 (95.88%), Postives = 383/388 (98.71%), Query Frame = 0

Query: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60
           MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH
Sbjct: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120
           SNFSSFIRQLNTYGFKKV P+QWEFANDDFVRG+QHLMKNIHRRKPVHSHSLQNPHGQGV
Sbjct: 61  SNFSSFIRQLNTYGFKKVHPEQWEFANDDFVRGKQHLMKNIHRRKPVHSHSLQNPHGQGV 120

Query: 121 SPPLTEVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQL 180
           SPPLTEVERKSFED+IETLKRDKEQ++LELRKH+QEYQGVVLQMQNLKDRFQCVQQGMQL
Sbjct: 121 SPPLTEVERKSFEDDIETLKRDKEQILLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180

Query: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENMD 240
           FISLIAR LHKPG RLDLLPQLETSDRKRRLPRVSYNNSEDN+EDNQMGTTQTISRENMD
Sbjct: 181 FISLIARLLHKPGRRLDLLPQLETSDRKRRLPRVSYNNSEDNIEDNQMGTTQTISRENMD 240

Query: 241 CSFDPILKEEQFELLETSIAFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300
           CSFDPILKEEQFEL+ETS+ FWEGI+HSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ
Sbjct: 241 CSFDPILKEEQFELVETSLTFWEGIVHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300

Query: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360
           VSEELRCKSPGIDMNLEPMATVAPES+ASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS
Sbjct: 301 VSEELRCKSPGIDMNLEPMATVAPESIASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360

Query: 361 DPQEVQSARKDSDVIIEENRPSDQGNFW 388
           DPQEVQSARKDSDVIIEENR SDQGNFW
Sbjct: 361 DPQEVQSARKDSDVIIEENRRSDQGNFW 388

BLAST of Cp4.1LG20g04750 vs. NCBI nr
Match: XP_038895068.1 (heat stress transcription factor A-4a [Benincasa hispida] >XP_038895070.1 heat stress transcription factor A-4a [Benincasa hispida])

HSP 1 Score: 697 bits (1799), Expect = 4.29e-251
Identity = 348/410 (84.88%), Postives = 372/410 (90.73%), Query Frame = 0

Query: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60
           MDEAQG GLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVW PLEFSSVLLPKFFKH
Sbjct: 1   MDEAQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120
           SNFSSFIRQLNTYGF+KVDP+QWEFANDDFVR + HLMKNIHRRKPVHSHSLQN HGQG+
Sbjct: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANDDFVRSKPHLMKNIHRRKPVHSHSLQNLHGQGI 120

Query: 121 SPPLTEVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQL 180
           SP LTEVER    D+IE LK DKEQL+LEL+KH+QEYQGV LQMQNLKDRFQCVQQ MQ 
Sbjct: 121 SP-LTEVERNGLNDDIERLKLDKEQLLLELQKHEQEYQGVGLQMQNLKDRFQCVQQEMQS 180

Query: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENMD 240
           FISL+AR L KPGL LDLLPQLET +RKRRLPRVSYNN+ED LEDNQMGTTQTI R++M 
Sbjct: 181 FISLMARILQKPGLHLDLLPQLETPERKRRLPRVSYNNNEDKLEDNQMGTTQTIGRDDMG 240

Query: 241 CSFDPILKEEQFELLETSIAFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300
           CSFD I K+EQFEL+ETS+ FWEGII SYGQT+SPLDSSSNLELGG VSHASSPA +CRQ
Sbjct: 241 CSFDSIFKKEQFELIETSLTFWEGIILSYGQTVSPLDSSSNLELGGCVSHASSPATSCRQ 300

Query: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360
           VSEE RCKSPGIDMNLEP+ TVAP+S+ASKDQ AGV APVPTG NDVFWQQFLTENPG+S
Sbjct: 301 VSEEFRCKSPGIDMNLEPVPTVAPDSLASKDQEAGVNAPVPTGANDVFWQQFLTENPGAS 360

Query: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKF 410
           DPQEVQSARKDSDVI +ENR SD G FWWNTRSVNNVVEQIG+L PAEKF
Sbjct: 361 DPQEVQSARKDSDVINDENRQSDHGKFWWNTRSVNNVVEQIGHLKPAEKF 409

BLAST of Cp4.1LG20g04750 vs. ExPASy TrEMBL
Match: A0A6J1EI67 (heat stress transcription factor A-4c-like OS=Cucurbita moschata OX=3662 GN=LOC111434310 PE=3 SV=1)

HSP 1 Score: 811 bits (2096), Expect = 1.24e-296
Identity = 405/411 (98.54%), Postives = 409/411 (99.51%), Query Frame = 0

Query: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60
           MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH
Sbjct: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120
           SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV
Sbjct: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120

Query: 121 SPPLTEVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQL 180
           SPPLTEVERKSFEDNIETLKRDKEQL+LELRKH+QEYQGVVLQMQNLKDRFQCVQQGMQL
Sbjct: 121 SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180

Query: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENMD 240
           FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISREN D
Sbjct: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD 240

Query: 241 CSFDPILKEEQFELLETSIAFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300
           CSFDPILKEEQFEL+ETS+ FWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ
Sbjct: 241 CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300

Query: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360
           VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS
Sbjct: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360

Query: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKFS 411
           DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKFS
Sbjct: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKFS 411

BLAST of Cp4.1LG20g04750 vs. ExPASy TrEMBL
Match: A0A6J1KEK1 (heat stress transcription factor A-4a-like OS=Cucurbita maxima OX=3661 GN=LOC111495117 PE=3 SV=1)

HSP 1 Score: 749 bits (1933), Expect = 3.63e-272
Identity = 372/388 (95.88%), Postives = 383/388 (98.71%), Query Frame = 0

Query: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60
           MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH
Sbjct: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120
           SNFSSFIRQLNTYGFKKV P+QWEFANDDFVRG+QHLMKNIHRRKPVHSHSLQNPHGQGV
Sbjct: 61  SNFSSFIRQLNTYGFKKVHPEQWEFANDDFVRGKQHLMKNIHRRKPVHSHSLQNPHGQGV 120

Query: 121 SPPLTEVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQL 180
           SPPLTEVERKSFED+IETLKRDKEQ++LELRKH+QEYQGVVLQMQNLKDRFQCVQQGMQL
Sbjct: 121 SPPLTEVERKSFEDDIETLKRDKEQILLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180

Query: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENMD 240
           FISLIAR LHKPG RLDLLPQLETSDRKRRLPRVSYNNSEDN+EDNQMGTTQTISRENMD
Sbjct: 181 FISLIARLLHKPGRRLDLLPQLETSDRKRRLPRVSYNNSEDNIEDNQMGTTQTISRENMD 240

Query: 241 CSFDPILKEEQFELLETSIAFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300
           CSFDPILKEEQFEL+ETS+ FWEGI+HSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ
Sbjct: 241 CSFDPILKEEQFELVETSLTFWEGIVHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300

Query: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360
           VSEELRCKSPGIDMNLEPMATVAPES+ASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS
Sbjct: 301 VSEELRCKSPGIDMNLEPMATVAPESIASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360

Query: 361 DPQEVQSARKDSDVIIEENRPSDQGNFW 388
           DPQEVQSARKDSDVIIEENR SDQGNFW
Sbjct: 361 DPQEVQSARKDSDVIIEENRRSDQGNFW 388

BLAST of Cp4.1LG20g04750 vs. ExPASy TrEMBL
Match: A0A0A0LV22 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G467710 PE=3 SV=1)

HSP 1 Score: 694 bits (1790), Expect = 4.88e-250
Identity = 347/410 (84.63%), Postives = 377/410 (91.95%), Query Frame = 0

Query: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60
           MDEAQG GLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVW PLEFSSVLLPKFFKH
Sbjct: 1   MDEAQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120
           SNFSSFIRQLNTYGF+KVDP+QWEFAN+DFVRG+ HLMKNIHRRKP+HSHSLQN HGQG+
Sbjct: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANEDFVRGKPHLMKNIHRRKPIHSHSLQNLHGQGI 120

Query: 121 SPPLTEVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQL 180
           SP LTEVER SF+D+IE LK DKEQL+LEL+K++QEYQGV LQ+QNLKDRFQ VQQ MQL
Sbjct: 121 SP-LTEVERNSFKDDIERLKLDKEQLLLELQKYEQEYQGVGLQIQNLKDRFQRVQQEMQL 180

Query: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENMD 240
           FISL+AR L KPGL LDLLPQLET +RKRRLPRVSYN SED+LEDN +GTTQTI R++M 
Sbjct: 181 FISLMARLLQKPGLHLDLLPQLETPERKRRLPRVSYNISEDSLEDNHLGTTQTIGRDDMG 240

Query: 241 CSFDPILKEEQFELLETSIAFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300
           CSFDPIL++EQ ELLETS+ FWEGIIHSY +T+SPLDSSSNLEL GSVSHASSPA++CR 
Sbjct: 241 CSFDPILEKEQLELLETSLTFWEGIIHSYDETVSPLDSSSNLELVGSVSHASSPAISCRL 300

Query: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360
           V EE RCKSPGIDMNLEPMATVAP+SVASKDQAAGV AP+PTG NDVFWQQFLTENPG+S
Sbjct: 301 VREEFRCKSPGIDMNLEPMATVAPDSVASKDQAAGVNAPLPTGFNDVFWQQFLTENPGAS 360

Query: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKF 410
           DPQEVQSARKDSDVI EENR SD G FWWNTRSVNNVVEQIG+L PAEKF
Sbjct: 361 DPQEVQSARKDSDVINEENRQSDHGKFWWNTRSVNNVVEQIGHLKPAEKF 409

BLAST of Cp4.1LG20g04750 vs. ExPASy TrEMBL
Match: A0A1S4DVL4 (heat stress transcription factor A-4c OS=Cucumis melo OX=3656 GN=LOC103487107 PE=3 SV=1)

HSP 1 Score: 692 bits (1787), Expect = 1.40e-249
Identity = 345/410 (84.15%), Postives = 375/410 (91.46%), Query Frame = 0

Query: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60
           MDEAQG GLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVW PLEFSSVLLPKFFKH
Sbjct: 1   MDEAQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120
           SNFSSFIRQLNTYGF+KVDP+QWEFAN+DFVRG+ HLMKNIHRRKP+HSHSLQN HGQG+
Sbjct: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANEDFVRGKPHLMKNIHRRKPIHSHSLQNLHGQGI 120

Query: 121 SPPLTEVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQL 180
           SP LTEVER SF+DNIE LK DKEQL+LEL+K++QEYQGV LQMQNLKDRFQ VQQGMQL
Sbjct: 121 SP-LTEVERNSFKDNIERLKLDKEQLLLELQKYEQEYQGVGLQMQNLKDRFQRVQQGMQL 180

Query: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENMD 240
           FI L+AR   KPGLRLDLLPQLET +RKRRLPR SYN SED+LED+Q+GTTQ I RE++ 
Sbjct: 181 FIGLMARLFQKPGLRLDLLPQLETPERKRRLPRASYNISEDSLEDDQLGTTQAIGREDLS 240

Query: 241 CSFDPILKEEQFELLETSIAFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300
           CSFDPIL++EQ ELLETS+ FWEGIIHSY QT+ PLDSSSNLEL GSVSHASSPA++CR 
Sbjct: 241 CSFDPILEKEQLELLETSLTFWEGIIHSYDQTVIPLDSSSNLELVGSVSHASSPAISCRL 300

Query: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360
           V EE RCKSPGIDMNLEPMATVAP+SVASKDQAAGV AP+PTG NDVFWQQFLTENPG+S
Sbjct: 301 VREEFRCKSPGIDMNLEPMATVAPDSVASKDQAAGVNAPLPTGFNDVFWQQFLTENPGAS 360

Query: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKF 410
           DPQEVQSARKDSDVI EEN+ SD  NFWWNTRSVNN+VEQIG+L PAEKF
Sbjct: 361 DPQEVQSARKDSDVINEENKQSDHENFWWNTRSVNNIVEQIGHLKPAEKF 409

BLAST of Cp4.1LG20g04750 vs. ExPASy TrEMBL
Match: A0A6J1CE08 (heat stress transcription factor A-4c-like OS=Momordica charantia OX=3673 GN=LOC111010635 PE=3 SV=1)

HSP 1 Score: 644 bits (1661), Expect = 2.07e-230
Identity = 327/413 (79.18%), Postives = 368/413 (89.10%), Query Frame = 0

Query: 1   MDEAQGSG-LTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFK 60
           MDEAQG G L+SLPPFLVKTYDMVDDPST+SIVSW+ S+KSFVV  PLEFSSVLLPKFFK
Sbjct: 1   MDEAQGGGGLSSLPPFLVKTYDMVDDPSTDSIVSWTPSNKSFVVRNPLEFSSVLLPKFFK 60

Query: 61  HSNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQG 120
           HSNFSSFIRQLNTYGF+KVDP+QWEFAN+DFVRG+ +LMKNIHRRKPVHSHSLQN HGQG
Sbjct: 61  HSNFSSFIRQLNTYGFRKVDPEQWEFANEDFVRGQPYLMKNIHRRKPVHSHSLQNIHGQG 120

Query: 121 VSPPLTEVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQ 180
           +S PLTEVERK  + +IE LK+DKEQL+LELR+H+QE+QGV LQMQNLKDRF+ +QQ MQ
Sbjct: 121 ISSPLTEVERKGLKGDIERLKQDKEQLLLELRRHEQEHQGVGLQMQNLKDRFEHMQQQMQ 180

Query: 181 LFISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMG-TTQTISREN 240
            FISL+      PGLRLDLLP+LET +RKRRLPR++YNN+ED LED+QMG TTQ+++REN
Sbjct: 181 TFISLVV-----PGLRLDLLPRLETPERKRRLPRIAYNNNEDKLEDDQMGGTTQSVAREN 240

Query: 241 MDCSFDPILKEEQFELLETSIAFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTC 300
           MDCSFDPILK EQFEL ETS+ FWEGIIHS+GQ +SPLDSSS LEL  S SHASSPA++ 
Sbjct: 241 MDCSFDPILKREQFELFETSLIFWEGIIHSFGQKVSPLDSSSYLELDESTSHASSPAMSH 300

Query: 301 RQVSEELRCKSPGIDMNLEP-MATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENP 360
           RQVSEE RCKSPGIDMNLEP +ATVAPESVAS+DQAAGV APVPTGVND FW+QFLTENP
Sbjct: 301 RQVSEEFRCKSPGIDMNLEPPVATVAPESVASRDQAAGVNAPVPTGVNDGFWEQFLTENP 360

Query: 361 GSSDPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEKF 410
           GSSDPQEVQSARKDS+V++EE R  D G FWWN RSVNNVVEQIG+L PAEKF
Sbjct: 361 GSSDPQEVQSARKDSNVVVEEKRQRDHGKFWWNVRSVNNVVEQIGHLTPAEKF 408

BLAST of Cp4.1LG20g04750 vs. TAIR 10
Match: AT4G18880.1 (heat shock transcription factor A4A )

HSP 1 Score: 342.4 bits (877), Expect = 4.9e-94
Identity = 195/415 (46.99%), Postives = 265/415 (63.86%), Query Frame = 0

Query: 1   MDE-AQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFK 60
           MDE   G   +SLPPFL KTY+MVDD S++SIVSWS S+KSF+VW P EFS  LLP+FFK
Sbjct: 1   MDENNHGVSSSSLPPFLTKTYEMVDDSSSDSIVSWSQSNKSFIVWNPPEFSRDLLPRFFK 60

Query: 61  HSNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQG 120
           H+NFSSFIRQLNTYGF+K DP+QWEFANDDFVRG+ HLMKNIHRRKPVHSHSL  P+ Q 
Sbjct: 61  HNNFSSFIRQLNTYGFRKADPEQWEFANDDFVRGQPHLMKNIHRRKPVHSHSL--PNLQA 120

Query: 121 VSPPLTEVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQ 180
              PLT+ ER    + IE L ++KE L+ EL K  +E +   +Q++ LK+R Q +++  +
Sbjct: 121 QLNPLTDSERVRMNNQIERLTKEKEGLLEELHKQDEEREVFEMQVKELKERLQHMEKRQK 180

Query: 181 LFISLIARFLHKPGLRLDLLPQL-ETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISREN 240
             +S +++ L KPGL L+L P + ET++RKRR PR+ +   E  LE+N+   T  + RE 
Sbjct: 181 TMVSFVSQVLEKPGLALNLSPCVPETNERKRRFPRIEFFPDEPMLEENK---TCVVVREE 240

Query: 241 MDCSFDPILKEEQFELLETSIAFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTC 300
              S     +E Q E LE+SIA WE ++    +++    S   L++  S +   SP ++C
Sbjct: 241 GSTSPSSHTREHQVEQLESSIAIWENLVSDSCESMLQSRSMMTLDVDESSTFPESPPLSC 300

Query: 301 RQVSEELRCKSPG----IDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLT 360
            Q+S + R KSP     IDMN EP  +    +VA+         P   G ND FWQQF +
Sbjct: 301 IQLSVDSRLKSPPSPRIIDMNCEPDGSKEQNTVAAP------PPPPVAGANDGFWQQFFS 360

Query: 361 ENPGSSDPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQIGNLAPAEK 410
           ENPGS++ +EVQ  RKD     ++         WWN+R+VN + EQ+G+L  +E+
Sbjct: 361 ENPGSTEQREVQLERKDD----KDKAGVRTEKCWWNSRNVNAITEQLGHLTSSER 400

BLAST of Cp4.1LG20g04750 vs. TAIR 10
Match: AT5G45710.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 301.2 bits (770), Expect = 1.3e-81
Identity = 182/401 (45.39%), Postives = 237/401 (59.10%), Query Frame = 0

Query: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60
           MDE  G G +SLPPFL KTY+MVDD S++S+V+WS ++KSF+V  P EFS  LLP+FFKH
Sbjct: 1   MDENNG-GSSSLPPFLTKTYEMVDDSSSDSVVAWSENNKSFIVKNPAEFSRDLLPRFFKH 60

Query: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120
            NFSSFIRQLNTYGF+KVDP++WEF NDDFVRG  +LMKNIHRRKPVHSHSL N   Q  
Sbjct: 61  KNFSSFIRQLNTYGFRKVDPEKWEFLNDDFVRGRPYLMKNIHRRKPVHSHSLVNLQAQN- 120

Query: 121 SPPLTEVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQL 180
             PLTE ER+S ED IE LK +KE L+ EL+  +QE +   LQ+  LKDR Q ++Q  + 
Sbjct: 121 --PLTESERRSMEDQIERLKNEKEGLLAELQNQEQERKEFELQVTTLKDRLQHMEQHQKS 180

Query: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENMD 240
            ++ +++ L KPGL L+    LE  +R++R             ++N +  + +       
Sbjct: 181 IVAYVSQVLGKPGLSLN----LENHERRKR-----------RFQENSLPPSSS------- 240

Query: 241 CSFDPILKEEQFELLETSIAFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300
                    EQ E LE+S+ FWE ++           S S  + G   S     A     
Sbjct: 241 -------HIEQVEKLESSLTFWENLV-----------SESCEKSGLQSSSMDHDAAESSL 300

Query: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVP-TGVNDVFWQQFLTENPGS 360
              + R KS  IDMN EP  TV               AP P TGVND FW+Q LTENPGS
Sbjct: 301 SIGDTRPKSSKIDMNSEPPVTVT--------------APAPKTGVNDDFWEQCLTENPGS 343

Query: 361 SDPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSVNNVVEQ 401
           ++ QEVQS R+D       N+  +Q  +WWN+ +VNN+ E+
Sbjct: 361 TEQQEVQSERRDVGNDNNGNKIGNQRTYWWNSGNVNNITEK 343

BLAST of Cp4.1LG20g04750 vs. TAIR 10
Match: AT5G16820.1 (heat shock factor 3 )

HSP 1 Score: 198.0 bits (502), Expect = 1.5e-50
Identity = 110/227 (48.46%), Postives = 145/227 (63.88%), Query Frame = 0

Query: 9   LTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKHSNFSSFIR 68
           + S+PPFL KTYDMVDDP TN +VSWSS + SFVVW   EFS VLLPK+FKH+NFSSF+R
Sbjct: 22  VNSVPPFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVR 81

Query: 69  QLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGVSP----PL 128
           QLNTYGF+KVDPD+WEFAN+ F+RG + L+K+I RRKP  SH  QN     V        
Sbjct: 82  QLNTYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKP--SHVQQNQQQTQVQSSSVGAC 141

Query: 129 TEVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQLFISL 188
            EV +   E+ +E LKRDK  L+ EL + +Q+ Q    Q+QN+  + Q ++Q  Q  +S 
Sbjct: 142 VEVGKFGIEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMSF 201

Query: 189 IARFLHKPGLRLDLLP--------QLETSDRKRRLPRVSYNNSEDNL 224
           +A+ +  PG    L+         Q+  S++KRRLP     N  DN+
Sbjct: 202 LAKAVQSPGFLNQLVQQNNNDGNRQIPGSNKKRRLPVDEQENRGDNV 246

BLAST of Cp4.1LG20g04750 vs. TAIR 10
Match: AT5G16820.2 (heat shock factor 3 )

HSP 1 Score: 198.0 bits (502), Expect = 1.5e-50
Identity = 110/227 (48.46%), Postives = 145/227 (63.88%), Query Frame = 0

Query: 9   LTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKHSNFSSFIR 68
           + S+PPFL KTYDMVDDP TN +VSWSS + SFVVW   EFS VLLPK+FKH+NFSSF+R
Sbjct: 22  VNSVPPFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVR 81

Query: 69  QLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGVSP----PL 128
           QLNTYGF+KVDPD+WEFAN+ F+RG + L+K+I RRKP  SH  QN     V        
Sbjct: 82  QLNTYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKP--SHVQQNQQQTQVQSSSVGAC 141

Query: 129 TEVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQLFISL 188
            EV +   E+ +E LKRDK  L+ EL + +Q+ Q    Q+QN+  + Q ++Q  Q  +S 
Sbjct: 142 VEVGKFGIEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMSF 201

Query: 189 IARFLHKPGLRLDLLP--------QLETSDRKRRLPRVSYNNSEDNL 224
           +A+ +  PG    L+         Q+  S++KRRLP     N  DN+
Sbjct: 202 LAKAVQSPGFLNQLVQQNNNDGNRQIPGSNKKRRLPVDEQENRGDNV 246

BLAST of Cp4.1LG20g04750 vs. TAIR 10
Match: AT3G02990.1 (heat shock transcription factor A1E )

HSP 1 Score: 197.2 bits (500), Expect = 2.6e-50
Identity = 146/451 (32.37%), Postives = 225/451 (49.89%), Query Frame = 0

Query: 9   LTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKHSNFSSFIR 68
           ++S+PPFL KTYDMVDDP T+ +VSWSS + SFVVW   EF+   LPK+FKH+NFSSF+R
Sbjct: 18  MSSIPPFLSKTYDMVDDPLTDDVVSWSSGNNSFVVWNVPEFAKQFLPKYFKHNNFSSFVR 77

Query: 69  QLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGVS-PPLTEV 128
           QLNTYGF+KVDPD+WEFAN+ F+RG++ ++K+I RRKP      Q P  Q  S     EV
Sbjct: 78  QLNTYGFRKVDPDRWEFANEGFLRGQKQILKSIVRRKPAQVQPPQQPQVQHSSVGACVEV 137

Query: 129 ERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQLFISLIAR 188
            +   E+ +E L+RDK  L+ EL + +Q+ Q     +QN+  +   ++Q  Q  +S +A+
Sbjct: 138 GKFGLEEEVERLQRDKNVLMQELVRLRQQQQVTEHHLQNVGQKVHVMEQRQQQMMSFLAK 197

Query: 189 FLHKPGLRLDLLPQ-------LETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISR--EN 248
            +  PG       Q       +  S++KRRLP     NS  +      G ++ I R   +
Sbjct: 198 AVQSPGFLNQFSQQSNEANQHISESNKKRRLPVEDQMNSGSH---GVNGLSRQIVRYQSS 257

Query: 249 MDCSFDPILKEEQFELLETSIAFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTC 308
           M+ + + +L++ Q     ++    E +  + G  +     +SN+   GS S+  SP VT 
Sbjct: 258 MNDATNTMLQQIQ---QMSNAPSHESLSSNNGSFLLGDVPNSNISDNGSSSN-GSPEVTL 317

Query: 309 RQVS-------------------EELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKA- 368
             VS                   + +    P    +L P    A  S +S     G +  
Sbjct: 318 ADVSSIPAGFYPAMKYHEPCETNQVMETNLPFSQGDLLPPTQGAAASGSSSSDLVGCETD 377

Query: 369 ------PVPT------------------GVNDVFWQQFLTENPGSSDPQEVQSARKDSDV 405
                 P+                     V D FW+QF+ E+P   +  E+ S   ++++
Sbjct: 378 NGECLDPIMAVLDGALELEADTLNELLPEVQDSFWEQFIGESPVIGETDELISGSVENEL 437

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O494036.9e-9346.99Heat stress transcription factor A-4a OS=Arabidopsis thaliana OX=3702 GN=HSFA4A ... [more]
Q9FK721.8e-8045.39Heat stress transcription factor A-4c OS=Arabidopsis thaliana OX=3702 GN=HSFA4C ... [more]
Q94J165.5e-7439.73Heat stress transcription factor A-4b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q93VB54.3e-5835.96Heat stress transcription factor A-4d OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q84T617.8e-5238.67Heat stress transcription factor A-1 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Match NameE-valueIdentityDescription
XP_023519250.11.14e-299100.00heat stress transcription factor A-4a-like [Cucurbita pepo subsp. pepo] >XP_0235... [more]
XP_022927503.12.57e-29698.54heat stress transcription factor A-4c-like [Cucurbita moschata] >XP_022927504.1 ... [more]
KAG6583940.11.98e-28198.47Heat stress transcription factor A-4a, partial [Cucurbita argyrosperma subsp. so... [more]
XP_023000762.17.50e-27295.88heat stress transcription factor A-4a-like [Cucurbita maxima] >XP_023000763.1 he... [more]
XP_038895068.14.29e-25184.88heat stress transcription factor A-4a [Benincasa hispida] >XP_038895070.1 heat s... [more]
Match NameE-valueIdentityDescription
A0A6J1EI671.24e-29698.54heat stress transcription factor A-4c-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1KEK13.63e-27295.88heat stress transcription factor A-4a-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A0A0LV224.88e-25084.63HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G467710 ... [more]
A0A1S4DVL41.40e-24984.15heat stress transcription factor A-4c OS=Cucumis melo OX=3656 GN=LOC103487107 PE... [more]
A0A6J1CE082.07e-23079.18heat stress transcription factor A-4c-like OS=Momordica charantia OX=3673 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT4G18880.14.9e-9446.99heat shock transcription factor A4A [more]
AT5G45710.11.3e-8145.39winged-helix DNA-binding transcription factor family protein [more]
AT5G16820.11.5e-5048.46heat shock factor 3 [more]
AT5G16820.21.5e-5048.46heat shock factor 3 [more]
AT3G02990.12.6e-5032.37heat shock transcription factor A1E [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 129..170
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 355..382
NoneNo IPR availablePANTHERPTHR10015:SF161HEAT STRESS TRANSCRIPTION FACTOR A-4Ccoord: 1..391
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 66..78
score: 78.68
coord: 15..38
score: 46.07
coord: 53..65
score: 72.09
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 11..104
e-value: 2.5E-57
score: 206.5
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 15..104
e-value: 3.1E-30
score: 104.6
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 54..78
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 9..105
e-value: 9.3E-36
score: 124.4
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 1..391
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 11..104

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG20g04750.1Cp4.1LG20g04750.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034605 cellular response to heat
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0043565 sequence-specific DNA binding