Cp4.1LG20g03400 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG20g03400
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Pectate lyase
Location	Cp4.1LG20: 1966050 .. 1967671 (+)
RNA-Seq Expression	Cp4.1LG20g03400
Synteny	Cp4.1LG20g03400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCATGGCTTCCTCATCATCCCACCTTCTTCTAACTCTTCTCATCATTATCTCACTCATACAATCCTCCCATCAAGATTACTACACCCCAACCAAGAACAACATGAACATAATCGACTCTTGTTGGCGTTCATCCTCCAATTGGGCCAAAAACCGCCAAGCCTTAGCCAACTGCGCGGTGGGCTACGGCAAGGACGCAATTGGAGGCAAATTTGGCACCATTTACGTAGTCACCGACCCATCTGACAACCCTTCAAACCCCAAATATGGAACGTTGCGTTATGGTGTGATTCAAAACAAGCCACTTTGGGTTGTGTTTGCTAAGAGCATGGTTATTGTGTTGAAGAATGAGCTGATGATAAATAGTTTCAAGACCATTGATGGACGTGGTGCTAATGTGGAGATTGCTTATGGGCCTTGCATTACGGTGCAAGGGGTTAGCCATGTTATTATACATGGAATTAGTATCCATGATTGTAAGCCTGCCAAAGCTGGGCTTGTTAGAGACACTGTCACACATGTTGGCAAACGTAGAGGCTCTGATGGTGATGCCATTTCTATATTTGCTTCCTCCCATGTTTGGATTGATCATTGTTACTTAGCTCAATCCACTGATGGTCTTATTGATATAATTCATGCTTCCACCGCTGTTACTGTCTCAAATAACTACTTCTCCCAACATGACAAGGTAAGAATGTTTTTTTAAGATTCTTTCTGTCAGATCCCATGTCAGTGTAAAAATTCTTTATAAGAGTGTAAAAATCTCTCCTTATTAACACATTCTAAAATTTGGGTTTCGTTTTTGATAGGTGATGCTGCTTGGACATAACGATGGGTTCACCGATGATAAAATTATGAGGGTCACCATTGTCTTCAACCGCTTCGGAGCTGGCCTTATCGAGAGGATGCCGAGGTAAAAAAAAAAAAAAACGCTATAAAATGTTCTCATACCAACATATGCAAGTCATACATTCATACTCAGAATTTACGAAAAAGTACTATAAAGATCTGCAATAACATTTTTTTTTGTTAGCTATCATATCCATTATTTCTTGTAGTGTAAATTAAAGACTAACCCTTTACTTATTATCGAAATTTCTTATAGTTTAAGACGAAATTTGTGATTTAACGAGTGTTATATGAATATCCCTAATGTTTTGTTATCTGGGTATTAATAATGATATAGGGTTAGGTTCGGTTACGCCCACGTAGCCAACAATCGATACGACGAGTGGAAAATGTACGCGATAGGTGGGAGTGCAAACCCCACAATCTTCAGTGAAGGGAACTACTTCGTAGCTCCCAAGAACTCAAATGCCAAACAGGTAATACATAGCAACAACCACTTCACTTCAAGAATCAAAAATTTAAATTTGGATGTAAAATAAAGCATTTGAACAACGAACAGGTAACAAAGAGAGAGGTCAACAAAGGGTGGAATAACTGGAAATGGAGATCCTCAAGAGACGTGTTCTTGAACGGCGCTTACTTTGTGCAATCTGGGTGGGGAAGTTGCAGCCCATTCTACACAAGAGCTCAGTCCTTTCCAGTGGCTCCAGGCTCCAAGGTTCCAGCTTTGACTGCAAATTCTGGGCCACTTCGTTGCTCCATTGGCAAACGTTGC

mRNA sequence

CCATGGCTTCCTCATCATCCCACCTTCTTCTAACTCTTCTCATCATTATCTCACTCATACAATCCTCCCATCAAGATTACTACACCCCAACCAAGAACAACATGAACATAATCGACTCTTGTTGGCGTTCATCCTCCAATTGGGCCAAAAACCGCCAAGCCTTAGCCAACTGCGCGGTGGGCTACGGCAAGGACGCAATTGGAGGCAAATTTGGCACCATTTACGTAGTCACCGACCCATCTGACAACCCTTCAAACCCCAAATATGGAACGTTGCGTTATGGTGTGATTCAAAACAAGCCACTTTGGGTTGTGTTTGCTAAGAGCATGGTTATTGTGTTGAAGAATGAGCTGATGATAAATAGTTTCAAGACCATTGATGGACGTGGTGCTAATGTGGAGATTGCTTATGGGCCTTGCATTACGGTGCAAGGGGTTAGCCATGTTATTATACATGGAATTAGTATCCATGATTGTAAGCCTGCCAAAGCTGGGCTTGTTAGAGACACTGTCACACATGTTGGCAAACGTAGAGGCTCTGATGGTGATGCCATTTCTATATTTGCTTCCTCCCATGTTTGGATTGATCATTGTTACTTAGCTCAATCCACTGATGCCAACAATCGATACGACGAGTGGAAAATGTACGCGATAGGTGGGAGTGCAAACCCCACAATCTTCAGTGAAGGGAACTACTTCGTAGCTCCCAAGAACTCAAATGCCAAACAGGTAACAAAGAGAGAGGTCAACAAAGGGTGGAATAACTGGAAATGGAGATCCTCAAGAGACGTGTTCTTGAACGGCGCTTACTTTGTGCAATCTGGGTGGGGAAGTTGCAGCCCATTCTACACAAGAGCTCAGTCCTTTCCAGTGGCTCCAGGCTCCAAGGTTCCAGCTTTGACTGCAAATTCTGGGCCACTTCGTTGCTCCATTGGCAAACGTTGC

Coding sequence (CDS)

ATGGCTTCCTCATCATCCCACCTTCTTCTAACTCTTCTCATCATTATCTCACTCATACAATCCTCCCATCAAGATTACTACACCCCAACCAAGAACAACATGAACATAATCGACTCTTGTTGGCGTTCATCCTCCAATTGGGCCAAAAACCGCCAAGCCTTAGCCAACTGCGCGGTGGGCTACGGCAAGGACGCAATTGGAGGCAAATTTGGCACCATTTACGTAGTCACCGACCCATCTGACAACCCTTCAAACCCCAAATATGGAACGTTGCGTTATGGTGTGATTCAAAACAAGCCACTTTGGGTTGTGTTTGCTAAGAGCATGGTTATTGTGTTGAAGAATGAGCTGATGATAAATAGTTTCAAGACCATTGATGGACGTGGTGCTAATGTGGAGATTGCTTATGGGCCTTGCATTACGGTGCAAGGGGTTAGCCATGTTATTATACATGGAATTAGTATCCATGATTGTAAGCCTGCCAAAGCTGGGCTTGTTAGAGACACTGTCACACATGTTGGCAAACGTAGAGGCTCTGATGGTGATGCCATTTCTATATTTGCTTCCTCCCATGTTTGGATTGATCATTGTTACTTAGCTCAATCCACTGATGCCAACAATCGATACGACGAGTGGAAAATGTACGCGATAGGTGGGAGTGCAAACCCCACAATCTTCAGTGAAGGGAACTACTTCGTAGCTCCCAAGAACTCAAATGCCAAACAGGTAACAAAGAGAGAGGTCAACAAAGGGTGGAATAACTGGAAATGGAGATCCTCAAGAGACGTGTTCTTGAACGGCGCTTACTTTGTGCAATCTGGGTGGGGAAGTTGCAGCCCATTCTACACAAGAGCTCAGTCCTTTCCAGTGGCTCCAGGCTCCAAGGTTCCAGCTTTGACTGCAAATTCTGGGCCACTTCGTTGCTCCATTGGCAAACGTTGC

Protein sequence

MASSSSHLLLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVGYGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMINSFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSDGDAISIFASSHVWIDHCYLAQSTDANNRYDEWKMYAIGGSANPTIFSEGNYFVAPKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPGSKVPALTANSGPLRCSIGKRC

Homology

[Close]

BLAST of Cp4.1LG20g03400 vs. ExPASy Swiss-Prot
Match: O65456 (Probable pectate lyase 16 OS=Arabidopsis thaliana OX=3702 GN=At4g22080 PE=2 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 3.7e-110
Identity = 205/387 (52.97%), Postives = 250/387 (64.60%), Query Frame = 0


Query: 6   SHLLLTLLIIISLIQSSHQDY--YTPT---------KNNMNIIDSCWRSSSNWAKNRQAL 65
           S LL+ L +  SL+ + +  Y  YTPT         +N MN +DSCWR  S+WA NR+ L
Sbjct: 7   SCLLVVLFLCHSLVHAENNGYYGYTPTVANYLPEKPQNIMNPVDSCWRLKSDWAANRKDL 66

Query: 66  ANCAVGYGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLK 125
           A+C VG+G   +GGK G +YVVT+P DN  NP+ G+LRYGVIQ KPLW+ FAK MVI L+
Sbjct: 67  ADCVVGFGSSTLGGKKGNLYVVTNPYDNAQNPQPGSLRYGVIQAKPLWITFAKDMVITLE 126

Query: 126 NELMINSFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVG 185
           NELM+NS+KTIDGRGA VEIAYGPCIT+Q V++VI+HGISIHDCKP K G+VR + THVG
Sbjct: 127 NELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVIVHGISIHDCKPGKYGMVRSSPTHVG 186

Query: 186 KRRGSDGDAISIFASSHVWIDHCYLAQSTD------------------------------ 245
            R+GSDGDAI+IF SS++WIDHCYLA  TD                              
Sbjct: 187 HRKGSDGDAIAIFGSSNIWIDHCYLASCTDGLIDVIHASTGITISNNYFTQHDKVMLLGH 246

Query: 246 -------------------------------------ANNRYDEWKMYAIGGSANPTIFS 305
                                                ANNRYD+W MYAIGGSA+PTIFS
Sbjct: 247 NDDFVQDVKMKVTVAFNHFGPGLVERMPRVRRGYAHVANNRYDKWIMYAIGGSADPTIFS 306

Query: 306 EGNYFVAPKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQS 315
           EGNYF+A   SN+K+VTKREV  GWNNW+WR+S+DVF NGAYFV SG+GS S  Y+ AQ 
Sbjct: 307 EGNYFIASDKSNSKEVTKREVKGGWNNWRWRTSKDVFKNGAYFVPSGYGSISLPYSSAQR 366

[Close]

BLAST of Cp4.1LG20g03400 vs. ExPASy Swiss-Prot
Match: O65388 (Putative pectate lyase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g11920 PE=3 SV=2)

HSP 1 Score: 393.7 bits (1010), Expect = 2.0e-108
Identity = 198/381 (51.97%), Postives = 253/381 (66.40%), Query Frame = 0


Query: 8   LLLTLLIII------SLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVGY 67
           L LT++ ++      S++++++ + YT  K   N IDSCWR +  WA NR+ALA+CAVG+
Sbjct: 4   LFLTIISLLFAAFSSSVVEAAYSNGYTIPKLLPNPIDSCWRRNPYWASNRRALADCAVGF 63

Query: 68  GKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMINS 127
           GK A+GGK+G+IYVVT+PSD+P NP+ GTLRY VIQ+KPLW+ FA+ MVIVL+NEL++NS
Sbjct: 64  GKSAVGGKYGSIYVVTNPSDDPENPRPGTLRYAVIQSKPLWITFARDMVIVLRNELIMNS 123

Query: 128 FKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSDG 187
           +KTIDGRGA VEIAYGPCIT+Q VSHVIIHGISIHDCKP K+G VR + THVG R+GSDG
Sbjct: 124 YKTIDGRGAKVEIAYGPCITIQHVSHVIIHGISIHDCKPGKSGRVRSSPTHVGSRKGSDG 183

Query: 188 DAISIFASSHVWIDHCYLAQSTD------------------------------------- 247
           DAI+IF SSH+WIDHC+ ++  D                                     
Sbjct: 184 DAIAIFDSSHIWIDHCFFSRCQDGLIDVLHASTAVTISNNYFTQHDKVMLLGHNDNNVED 243

Query: 248 ------------------------------ANNRYDEWKMYAIGGSANPTIFSEGNYFVA 307
                                         ANNRY++W+MYAIGGSA+PTIFSEGNYFVA
Sbjct: 244 KIMRVTIAFNHFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSADPTIFSEGNYFVA 303

Query: 308 PKNSNAKQVTKR-EVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG 315
             + + KQVTKR +    W  WKWR+S+DVF NGAYFV SG+G+ +P Y RA+ FPV+ G
Sbjct: 304 SDDPSKKQVTKRIDSGYDWKRWKWRTSKDVFKNGAYFVPSGYGTVTPLYGRAERFPVSHG 363

[Close]

BLAST of Cp4.1LG20g03400 vs. ExPASy Swiss-Prot
Match: O65457 (Putative pectate lyase 17 OS=Arabidopsis thaliana OX=3702 GN=At4g22090 PE=3 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 1.1e-106
Identity = 201/387 (51.94%), Postives = 243/387 (62.79%), Query Frame = 0


Query: 6   SHLLLTLLIIISLIQSSHQDYY-----------TPTKNNMNIIDSCWRSSSNWAKNRQAL 65
           S LL+ L +  SL+ +++  YY              +N MN +DSCWR  S+W  NR+ L
Sbjct: 7   SCLLVALFLCQSLVHAAYNGYYGYSPAAAPYPAEEPQNIMNPVDSCWRLKSDWDVNREDL 66

Query: 66  ANCAVGYGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLK 125
           A+CAVG+G   +GGK G IYVVT+P DN  NP  G+LRYGVIQ KPLW+ FAK MVI L 
Sbjct: 67  ADCAVGFGSSTLGGKKGNIYVVTNPYDNAQNPHPGSLRYGVIQAKPLWITFAKDMVITLA 126

Query: 126 NELMINSFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVG 185
           NELM+NS+KTIDGRGA VEIAYGPCIT+Q V++VI+HGISIHDCKP K+G VR + THVG
Sbjct: 127 NELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVIVHGISIHDCKPGKSGKVRSSPTHVG 186

Query: 186 KRRGSDGDAISIFASSHVWIDHCYLAQSTD------------------------------ 245
            R+GSDGDAI+IF SS+VWIDHCYLA  TD                              
Sbjct: 187 HRKGSDGDAITIFGSSNVWIDHCYLASCTDGLIDVIHASTAITISNNYFTQHDKVMLLGH 246

Query: 246 -------------------------------------ANNRYDEWKMYAIGGSANPTIFS 305
                                                ANNRYD+W MYAIGGSA+PTIFS
Sbjct: 247 NDNFVKDVKMKVTVAFNHFGPGLVERMPRVRRGYAHVANNRYDKWIMYAIGGSADPTIFS 306

Query: 306 EGNYFVAPKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQS 315
           EGNYF+A   S +K+VTKREV  GWNNW+WR+S DVF NGA+FV SG+GS    Y+ AQ 
Sbjct: 307 EGNYFIASDKSYSKEVTKREVKGGWNNWRWRTSNDVFKNGAFFVPSGYGSIPLPYSSAQR 366

[Close]

BLAST of Cp4.1LG20g03400 vs. ExPASy Swiss-Prot
Match: Q9C8G4 (Probable pectate lyase 4 OS=Arabidopsis thaliana OX=3702 GN=At1g30350 PE=2 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 2.6e-87
Identity = 176/374 (47.06%), Postives = 218/374 (58.29%), Query Frame = 0


Query: 9   LLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVGYGKDAIGG 68
           LL       L++++H    T  + ++N ID+CWR +  WA NRQALA+CAVGYGK AIGG
Sbjct: 10  LLLAAFASPLLETAHSYNVTAPRVSLNPIDACWRRNPKWATNRQALAHCAVGYGKAAIGG 69

Query: 69  KFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMINSFKTIDGR 128
           K G IYVVT+PSDNP+ P  GTLRY V Q KPLW+ FA+ MVIVLK++LMINS+KTIDGR
Sbjct: 70  KNGPIYVVTNPSDNPTRPSPGTLRYAVSQPKPLWITFARDMVIVLKSQLMINSYKTIDGR 129

Query: 129 GANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSDGDAISIFA 188
           GA VEIA GPC+ ++ V HVIIHGISIHDCK                  G DGD I +F 
Sbjct: 130 GAKVEIANGPCLRIRQVKHVIIHGISIHDCK--------------ADPNGMDGDGIRVFQ 189

Query: 189 SSHVWIDHCYLAQSTD-------------------------------------------- 248
           S+HVWIDHC+L++  D                                            
Sbjct: 190 STHVWIDHCFLSRCHDGLIDVIVSSTAVTISNNYFTQHDKVMLLGHDDSYMGDKDMRVTI 249

Query: 249 -----------------------ANNRYDEWKMYAIGGSANPTIFSEGNYFVAPKNSNAK 308
                                  ANNRY++W+MYAIGGSANP IFSEGNYFVAP+  ++K
Sbjct: 250 AFNTFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSANPIIFSEGNYFVAPEKRSSK 309

Query: 309 QVTKREV-NKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPGSKVPALT 315
           QVTKR +       WKW +SRDVF+NGA+F   G     P Y   + F VA GS VP+LT
Sbjct: 310 QVTKRMMAGPDSKRWKWGTSRDVFMNGAFFGPPG-VIVRPLYKGGEGFRVAHGSLVPSLT 368

[Close]

BLAST of Cp4.1LG20g03400 vs. ExPASy Swiss-Prot
Match: Q93Z25 (Probable pectate lyase 22 OS=Arabidopsis thaliana OX=3702 GN=At5g63180 PE=2 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 5.2e-80
Identity = 157/351 (44.73%), Postives = 198/351 (56.41%), Query Frame = 0


Query: 35  NIIDSCWRSSSNWAKNRQALANCAVGYGKDAIGGKFGTIYVVTDP-SDNPSNPKYGTLRY 94
           N ID CWR   +W KNR+ LA+C +G+GK+AIGG+ G IYVVTDP +D+P NP+ GTLRY
Sbjct: 82  NPIDDCWRCDKDWEKNRKRLADCGIGFGKNAIGGRDGEIYVVTDPGNDDPVNPRPGTLRY 141

Query: 95  GVIQNKPLWVVFAKSMVIVLKNELMINSFKTIDGRGANVEIAYGPCITVQGVSHVIIHGI 154
            VIQ++PLW++F + M I LK EL++NSFKT+DGRGA+V I+ GPCIT+Q V+++IIHG+
Sbjct: 142 AVIQDEPLWIIFKRDMTIQLKEELIMNSFKTLDGRGASVHISGGPCITIQYVTNIIIHGL 201

Query: 155 SIHDCKPAKAGLVRDTVTHVGKRRGSDGDAISIFASSHVWIDHCYLAQSTD--------- 214
            IHDCK      VRD+  H G R  SDGD +SIF  SHVW+DHC L+   D         
Sbjct: 202 HIHDCKQGGNTYVRDSPEHYGYRTVSDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIRGS 261

Query: 215 ----------------------------------------------------------AN 274
                                                                      N
Sbjct: 262 TAITISNNYLTHHNKVMLLGHSDTYEQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVN 321

Query: 275 NRYDEWKMYAIGGSANPTIFSEGNYFVAPKNSNAKQVTKRE--VNKGWNNWKWRSSRDVF 315
           N Y  W+MYAIGGSANPTI S+GN F+AP +S++K+VTK E      W NW WRS  D+ 
Sbjct: 322 NDYTHWEMYAIGGSANPTINSQGNRFLAPDDSSSKEVTKHEDAPEDEWRNWNWRSEGDLL 381

[Close]

BLAST of Cp4.1LG20g03400 vs. NCBI nr
Match: XP_023520154.1 (putative pectate lyase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 620 bits (1600), Expect = 6.44e-223
Identity = 314/381 (82.41%), Postives = 314/381 (82.41%), Query Frame = 0


Query: 1   MASSSSHLLLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG 60
           MASSSSHLLLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG
Sbjct: 1   MASSSSHLLLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG 60

Query: 61  YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN 120
           YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN
Sbjct: 61  YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN 120

Query: 121 SFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSD 180
           SFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSD
Sbjct: 121 SFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSD 180

Query: 181 GDAISIFASSHVWIDHCYLAQSTD------------------------------------ 240
           GDAISIFASSHVWIDHCYLAQSTD                                    
Sbjct: 181 GDAISIFASSHVWIDHCYLAQSTDGLIDIIHASTAVTVSNNYFSQHDKVMLLGHNDGFTD 240

Query: 241 -------------------------------ANNRYDEWKMYAIGGSANPTIFSEGNYFV 300
                                          ANNRYDEWKMYAIGGSANPTIFSEGNYFV
Sbjct: 241 DKIMRVTIVFNRFGAGLIERMPRVRFGYAHVANNRYDEWKMYAIGGSANPTIFSEGNYFV 300

Query: 301 APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG 314
           APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG
Sbjct: 301 APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG 360

[Close]

BLAST of Cp4.1LG20g03400 vs. NCBI nr
Match: KAG7019700.1 (putative pectate lyase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 619 bits (1596), Expect = 2.62e-222
Identity = 313/381 (82.15%), Postives = 314/381 (82.41%), Query Frame = 0


Query: 1   MASSSSHLLLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG 60
           MASSSSHLLLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG
Sbjct: 1   MASSSSHLLLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG 60

Query: 61  YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN 120
           YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN
Sbjct: 61  YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN 120

Query: 121 SFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSD 180
           SFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSD
Sbjct: 121 SFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSD 180

Query: 181 GDAISIFASSHVWIDHCYLAQSTD------------------------------------ 240
           GDAISIFASSHVWIDHCYLA+STD                                    
Sbjct: 181 GDAISIFASSHVWIDHCYLARSTDGLIDIIHASTAVTVSNNYFSQHDKVMLLGHNDGFTD 240

Query: 241 -------------------------------ANNRYDEWKMYAIGGSANPTIFSEGNYFV 300
                                          ANNRYDEWKMYAIGGSANPTIFSEGNYFV
Sbjct: 241 DKIMRVTIVFNRFGAGLIERMPRVRFGYAHVANNRYDEWKMYAIGGSANPTIFSEGNYFV 300

Query: 301 APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG 314
           APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG
Sbjct: 301 APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG 360

[Close]

BLAST of Cp4.1LG20g03400 vs. NCBI nr
Match: XP_022923701.1 (putative pectate lyase 2 [Cucurbita moschata])

HSP 1 Score: 617 bits (1590), Expect = 2.15e-221
Identity = 312/381 (81.89%), Postives = 314/381 (82.41%), Query Frame = 0


Query: 1   MASSSSHLLLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG 60
           MASSSSHLLLTLLIIISLIQSS+QDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG
Sbjct: 1   MASSSSHLLLTLLIIISLIQSSYQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG 60

Query: 61  YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN 120
           YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN
Sbjct: 61  YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN 120

Query: 121 SFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSD 180
           SFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSD
Sbjct: 121 SFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSD 180

Query: 181 GDAISIFASSHVWIDHCYLAQSTD------------------------------------ 240
           GDAISIFASSHVWIDHCYLA+STD                                    
Sbjct: 181 GDAISIFASSHVWIDHCYLARSTDGLIDIIHASTAVTVSNNYFSQHDKVMLLGHNDGFTD 240

Query: 241 -------------------------------ANNRYDEWKMYAIGGSANPTIFSEGNYFV 300
                                          ANNRYDEWKMYAIGGSANPTIFSEGNYFV
Sbjct: 241 DKIMRVTIVFNRFGAGLIERMPRVRFGYAHVANNRYDEWKMYAIGGSANPTIFSEGNYFV 300

Query: 301 APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG 314
           APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG
Sbjct: 301 APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG 360

[Close]

BLAST of Cp4.1LG20g03400 vs. NCBI nr
Match: KAG6584109.1 (putative pectate lyase 16, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 615 bits (1585), Expect = 1.24e-220
Identity = 311/381 (81.63%), Postives = 312/381 (81.89%), Query Frame = 0


Query: 1   MASSSSHLLLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG 60
           MASSSSHLLLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG
Sbjct: 1   MASSSSHLLLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG 60

Query: 61  YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN 120
           YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN
Sbjct: 61  YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN 120

Query: 121 SFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSD 180
           SFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSD
Sbjct: 121 SFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSD 180

Query: 181 GDAISIFASSHVWIDHCYLAQSTD------------------------------------ 240
           GDAISIF SSHVWIDHCYLA+STD                                    
Sbjct: 181 GDAISIFGSSHVWIDHCYLARSTDGLIDIIHASTAVTVSNNYFSQHDKVMLLGHNDGFTD 240

Query: 241 -------------------------------ANNRYDEWKMYAIGGSANPTIFSEGNYFV 300
                                          ANNRYDEWKMYAIGGSANPTIFSEGNYFV
Sbjct: 241 DKIMRVTIVFNRFGAGLIERMPRVRFGYAHVANNRYDEWKMYAIGGSANPTIFSEGNYFV 300

Query: 301 APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG 314
           APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG
Sbjct: 301 APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG 360

[Close]

BLAST of Cp4.1LG20g03400 vs. NCBI nr
Match: XP_023001281.1 (probable pectate lyase 16 [Cucurbita maxima])

HSP 1 Score: 609 bits (1571), Expect = 1.69e-218
Identity = 308/381 (80.84%), Postives = 311/381 (81.63%), Query Frame = 0


Query: 1   MASSSSHLLLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG 60
           MASSSSHLLLTLLII+SLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG
Sbjct: 1   MASSSSHLLLTLLIILSLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG 60

Query: 61  YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN 120
           YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN
Sbjct: 61  YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN 120

Query: 121 SFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSD 180
           SFKTIDGRGANVEIAYGPCITVQ VSHVIIHGISIHDCKPAKAGLVRD+VTHVGKRRGSD
Sbjct: 121 SFKTIDGRGANVEIAYGPCITVQRVSHVIIHGISIHDCKPAKAGLVRDSVTHVGKRRGSD 180

Query: 181 GDAISIFASSHVWIDHCYLAQSTD------------------------------------ 240
           GDAISIF SSHVWIDHCYLA+STD                                    
Sbjct: 181 GDAISIFGSSHVWIDHCYLARSTDGLIDIIHASTAVTVSNNYFSQHDKVMLLGHNDGFTD 240

Query: 241 -------------------------------ANNRYDEWKMYAIGGSANPTIFSEGNYFV 300
                                          ANNRYDEWKMYAIGGSANPTIFSEGNYFV
Sbjct: 241 DKIMRVTIVFNRFGAGLIERMPRVRFGYTHVANNRYDEWKMYAIGGSANPTIFSEGNYFV 300

Query: 301 APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG 314
           APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG
Sbjct: 301 APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG 360

[Close]

BLAST of Cp4.1LG20g03400 vs. ExPASy TrEMBL
Match: A0A6J1E7G9 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111431331 PE=3 SV=1)

HSP 1 Score: 617 bits (1590), Expect = 1.04e-221
Identity = 312/381 (81.89%), Postives = 314/381 (82.41%), Query Frame = 0


Query: 1   MASSSSHLLLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG 60
           MASSSSHLLLTLLIIISLIQSS+QDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG
Sbjct: 1   MASSSSHLLLTLLIIISLIQSSYQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG 60

Query: 61  YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN 120
           YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN
Sbjct: 61  YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN 120

Query: 121 SFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSD 180
           SFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSD
Sbjct: 121 SFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSD 180

Query: 181 GDAISIFASSHVWIDHCYLAQSTD------------------------------------ 240
           GDAISIFASSHVWIDHCYLA+STD                                    
Sbjct: 181 GDAISIFASSHVWIDHCYLARSTDGLIDIIHASTAVTVSNNYFSQHDKVMLLGHNDGFTD 240

Query: 241 -------------------------------ANNRYDEWKMYAIGGSANPTIFSEGNYFV 300
                                          ANNRYDEWKMYAIGGSANPTIFSEGNYFV
Sbjct: 241 DKIMRVTIVFNRFGAGLIERMPRVRFGYAHVANNRYDEWKMYAIGGSANPTIFSEGNYFV 300

Query: 301 APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG 314
           APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG
Sbjct: 301 APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG 360

[Close]

BLAST of Cp4.1LG20g03400 vs. ExPASy TrEMBL
Match: A0A6J1KI65 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111495456 PE=3 SV=1)

HSP 1 Score: 609 bits (1571), Expect = 8.17e-219
Identity = 308/381 (80.84%), Postives = 311/381 (81.63%), Query Frame = 0


Query: 1   MASSSSHLLLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG 60
           MASSSSHLLLTLLII+SLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG
Sbjct: 1   MASSSSHLLLTLLIILSLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG 60

Query: 61  YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN 120
           YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN
Sbjct: 61  YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN 120

Query: 121 SFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSD 180
           SFKTIDGRGANVEIAYGPCITVQ VSHVIIHGISIHDCKPAKAGLVRD+VTHVGKRRGSD
Sbjct: 121 SFKTIDGRGANVEIAYGPCITVQRVSHVIIHGISIHDCKPAKAGLVRDSVTHVGKRRGSD 180

Query: 181 GDAISIFASSHVWIDHCYLAQSTD------------------------------------ 240
           GDAISIF SSHVWIDHCYLA+STD                                    
Sbjct: 181 GDAISIFGSSHVWIDHCYLARSTDGLIDIIHASTAVTVSNNYFSQHDKVMLLGHNDGFTD 240

Query: 241 -------------------------------ANNRYDEWKMYAIGGSANPTIFSEGNYFV 300
                                          ANNRYDEWKMYAIGGSANPTIFSEGNYFV
Sbjct: 241 DKIMRVTIVFNRFGAGLIERMPRVRFGYTHVANNRYDEWKMYAIGGSANPTIFSEGNYFV 300

Query: 301 APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG 314
           APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG
Sbjct: 301 APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG 360

[Close]

BLAST of Cp4.1LG20g03400 vs. ExPASy TrEMBL
Match: A0A0A0LU35 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_1G059200 PE=3 SV=1)

HSP 1 Score: 553 bits (1425), Expect = 1.41e-196
Identity = 274/381 (71.92%), Postives = 291/381 (76.38%), Query Frame = 0


Query: 1   MASSSSHLLLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG 60
           MAS+SSHLLL LL I+SLI  S QDYYTP K NMN+IDSCWR +SNWAKNR+ALA+CAVG
Sbjct: 1   MASNSSHLLLILLFILSLINPSLQDYYTPQKKNMNVIDSCWRQTSNWAKNRKALADCAVG 60

Query: 61  YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN 120
           YGKDAIGGKFGTIY VTDPSDNPSNPKYGTLRYGVIQ+KPLW+VF K MVIVLKNELM+N
Sbjct: 61  YGKDAIGGKFGTIYTVTDPSDNPSNPKYGTLRYGVIQDKPLWIVFGKDMVIVLKNELMVN 120

Query: 121 SFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSD 180
           SFKTIDGRGA VEIAYGPCITVQGVSHVIIHGISIHDCKP KAGLVRDTV+H+GKR+GSD
Sbjct: 121 SFKTIDGRGAKVEIAYGPCITVQGVSHVIIHGISIHDCKPGKAGLVRDTVSHIGKRKGSD 180

Query: 181 GDAISIFASSHVWIDHCYLAQSTD------------------------------------ 240
           GDAI++F SSHVWIDHCYLA+ TD                                    
Sbjct: 181 GDAIAVFGSSHVWIDHCYLARCTDGLIDVIHASTSVTISNNYFSQHDKVMLLGHNDGFTD 240

Query: 241 -------------------------------ANNRYDEWKMYAIGGSANPTIFSEGNYFV 300
                                          ANNRYDEWKMYAIGGSANPTIFSEGNYFV
Sbjct: 241 DKIMRVTIVFNRFGAGLIERMPRVRFGYAHVANNRYDEWKMYAIGGSANPTIFSEGNYFV 300

Query: 301 APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG 314
           AP+NS AKQVTKREVN GW NWKWRSSRDVFLNGAYFV SGWGSCSP YT+AQSFPVAPG
Sbjct: 301 APQNSYAKQVTKREVNNGWKNWKWRSSRDVFLNGAYFVPSGWGSCSPIYTKAQSFPVAPG 360

[Close]

BLAST of Cp4.1LG20g03400 vs. ExPASy TrEMBL
Match: A0A1S3B1W2 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103485080 PE=3 SV=1)

HSP 1 Score: 540 bits (1391), Expect = 2.11e-191
Identity = 269/381 (70.60%), Postives = 285/381 (74.80%), Query Frame = 0


Query: 1   MASSSSHLLLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVG 60
           M S SSHLLL LL I S I  S Q YYTP K NMN+IDSCWR +SNWAKNR+ALA+CAVG
Sbjct: 1   MDSKSSHLLLILLFIFSFINPSLQGYYTPDKKNMNVIDSCWRQTSNWAKNRKALADCAVG 60

Query: 61  YGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMIN 120
           YGKDAIGGKFGTIY VTDPSDNPSNPKYGTLRYGVIQ+KPLW+VF K MVIVLKNELM+N
Sbjct: 61  YGKDAIGGKFGTIYTVTDPSDNPSNPKYGTLRYGVIQDKPLWIVFGKDMVIVLKNELMVN 120

Query: 121 SFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSD 180
           SFKTIDGRGA VEIAYGPCITVQGVSHVIIHGISIHDCKP K+GLVRDTV+HVGKR+GSD
Sbjct: 121 SFKTIDGRGAKVEIAYGPCITVQGVSHVIIHGISIHDCKPGKSGLVRDTVSHVGKRKGSD 180

Query: 181 GDAISIFASSHVWIDHCYLAQSTD------------------------------------ 240
           GDAI++F SSHVWIDHCYLA+ TD                                    
Sbjct: 181 GDAIAVFGSSHVWIDHCYLARCTDGLIDVIHASTSVTISNNYFSQHDKVMLLGHNDGFTD 240

Query: 241 -------------------------------ANNRYDEWKMYAIGGSANPTIFSEGNYFV 300
                                          ANNRYDEWKMYAIGGSANPTIFSEGNYFV
Sbjct: 241 DKIMRVTIVFNRFGAGLIERMPRVRFGYAHVANNRYDEWKMYAIGGSANPTIFSEGNYFV 300

Query: 301 APKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG 314
           A +NS AKQVTKREVN GW NWKWRSSRDVFLNGAYFV SGWGSCSP YT+AQSFPVAPG
Sbjct: 301 ASQNSYAKQVTKREVNNGWKNWKWRSSRDVFLNGAYFVPSGWGSCSPIYTKAQSFPVAPG 360

[Close]

BLAST of Cp4.1LG20g03400 vs. ExPASy TrEMBL
Match: A0A6J1C888 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111008879 PE=3 SV=1)

HSP 1 Score: 533 bits (1373), Expect = 1.20e-188
Identity = 265/382 (69.37%), Postives = 289/382 (75.65%), Query Frame = 0


Query: 1   MASSSS-HLLLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAV 60
           MAS+ + HL+L L++++S I SS QDYYTP + NMN+IDSCWR  SNWA NR+ALA+CA+
Sbjct: 1   MASTCTPHLVLCLVLVLSFINSSLQDYYTPDRQNMNVIDSCWRRRSNWAANRRALADCAI 60

Query: 61  GYGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMI 120
           GYG DAIGGKFGTIYVVTDPSDNPSNPK GTLRYGVIQ KPLWVVFAK+MVIVLKNELM+
Sbjct: 61  GYGNDAIGGKFGTIYVVTDPSDNPSNPKPGTLRYGVIQTKPLWVVFAKNMVIVLKNELMV 120

Query: 121 NSFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGS 180
           NSFKTIDGRG  VEIAYGPCITVQGVSHVIIHGISIHDCKP K GLVRDTVTHVG+R GS
Sbjct: 121 NSFKTIDGRGVKVEIAYGPCITVQGVSHVIIHGISIHDCKPGKPGLVRDTVTHVGERHGS 180

Query: 181 DGDAISIFASSHVWIDHCYLAQSTD----------------------------------- 240
           DGDAI+IF+SSHVWIDHC+LA+S+D                                   
Sbjct: 181 DGDAIAIFSSSHVWIDHCFLARSSDGLIDVIHASTAVTISNNYFSQHDKVMLLGHNDGFT 240

Query: 241 --------------------------------ANNRYDEWKMYAIGGSANPTIFSEGNYF 300
                                           ANNRYDEWKMYAIGGSANPTIFSEGNYF
Sbjct: 241 DDKIMRVTIVFNRFGAGLIERMPRVRFGYAHVANNRYDEWKMYAIGGSANPTIFSEGNYF 300

Query: 301 VAPKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAP 314
           VAP+NSNAKQVTKREVN GW NWKWRSSRDVFLNGAYFVQSGWGSCSP Y+RAQSFPVAP
Sbjct: 301 VAPQNSNAKQVTKREVNNGWKNWKWRSSRDVFLNGAYFVQSGWGSCSPIYSRAQSFPVAP 360

[Close]

BLAST of Cp4.1LG20g03400 vs. TAIR 10
Match: AT4G22080.1 (root hair specific 14 )

HSP 1 Score: 399.4 bits (1025), Expect = 2.6e-111
Identity = 205/387 (52.97%), Postives = 250/387 (64.60%), Query Frame = 0


Query: 6   SHLLLTLLIIISLIQSSHQDY--YTPT---------KNNMNIIDSCWRSSSNWAKNRQAL 65
           S LL+ L +  SL+ + +  Y  YTPT         +N MN +DSCWR  S+WA NR+ L
Sbjct: 7   SCLLVVLFLCHSLVHAENNGYYGYTPTVANYLPEKPQNIMNPVDSCWRLKSDWAANRKDL 66

Query: 66  ANCAVGYGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLK 125
           A+C VG+G   +GGK G +YVVT+P DN  NP+ G+LRYGVIQ KPLW+ FAK MVI L+
Sbjct: 67  ADCVVGFGSSTLGGKKGNLYVVTNPYDNAQNPQPGSLRYGVIQAKPLWITFAKDMVITLE 126

Query: 126 NELMINSFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVG 185
           NELM+NS+KTIDGRGA VEIAYGPCIT+Q V++VI+HGISIHDCKP K G+VR + THVG
Sbjct: 127 NELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVIVHGISIHDCKPGKYGMVRSSPTHVG 186

Query: 186 KRRGSDGDAISIFASSHVWIDHCYLAQSTD------------------------------ 245
            R+GSDGDAI+IF SS++WIDHCYLA  TD                              
Sbjct: 187 HRKGSDGDAIAIFGSSNIWIDHCYLASCTDGLIDVIHASTGITISNNYFTQHDKVMLLGH 246

Query: 246 -------------------------------------ANNRYDEWKMYAIGGSANPTIFS 305
                                                ANNRYD+W MYAIGGSA+PTIFS
Sbjct: 247 NDDFVQDVKMKVTVAFNHFGPGLVERMPRVRRGYAHVANNRYDKWIMYAIGGSADPTIFS 306

Query: 306 EGNYFVAPKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQS 315
           EGNYF+A   SN+K+VTKREV  GWNNW+WR+S+DVF NGAYFV SG+GS S  Y+ AQ 
Sbjct: 307 EGNYFIASDKSNSKEVTKREVKGGWNNWRWRTSKDVFKNGAYFVPSGYGSISLPYSSAQR 366

[Close]

BLAST of Cp4.1LG20g03400 vs. TAIR 10
Match: AT1G11920.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 393.7 bits (1010), Expect = 1.4e-109
Identity = 198/381 (51.97%), Postives = 253/381 (66.40%), Query Frame = 0


Query: 8   LLLTLLIII------SLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVGY 67
           L LT++ ++      S++++++ + YT  K   N IDSCWR +  WA NR+ALA+CAVG+
Sbjct: 4   LFLTIISLLFAAFSSSVVEAAYSNGYTIPKLLPNPIDSCWRRNPYWASNRRALADCAVGF 63

Query: 68  GKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMINS 127
           GK A+GGK+G+IYVVT+PSD+P NP+ GTLRY VIQ+KPLW+ FA+ MVIVL+NEL++NS
Sbjct: 64  GKSAVGGKYGSIYVVTNPSDDPENPRPGTLRYAVIQSKPLWITFARDMVIVLRNELIMNS 123

Query: 128 FKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSDG 187
           +KTIDGRGA VEIAYGPCIT+Q VSHVIIHGISIHDCKP K+G VR + THVG R+GSDG
Sbjct: 124 YKTIDGRGAKVEIAYGPCITIQHVSHVIIHGISIHDCKPGKSGRVRSSPTHVGSRKGSDG 183

Query: 188 DAISIFASSHVWIDHCYLAQSTD------------------------------------- 247
           DAI+IF SSH+WIDHC+ ++  D                                     
Sbjct: 184 DAIAIFDSSHIWIDHCFFSRCQDGLIDVLHASTAVTISNNYFTQHDKVMLLGHNDNNVED 243

Query: 248 ------------------------------ANNRYDEWKMYAIGGSANPTIFSEGNYFVA 307
                                         ANNRY++W+MYAIGGSA+PTIFSEGNYFVA
Sbjct: 244 KIMRVTIAFNHFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSADPTIFSEGNYFVA 303

Query: 308 PKNSNAKQVTKR-EVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPG 315
             + + KQVTKR +    W  WKWR+S+DVF NGAYFV SG+G+ +P Y RA+ FPV+ G
Sbjct: 304 SDDPSKKQVTKRIDSGYDWKRWKWRTSKDVFKNGAYFVPSGYGTVTPLYGRAERFPVSHG 363

[Close]

BLAST of Cp4.1LG20g03400 vs. TAIR 10
Match: AT4G22090.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 387.9 bits (995), Expect = 7.8e-108
Identity = 201/387 (51.94%), Postives = 243/387 (62.79%), Query Frame = 0


Query: 6   SHLLLTLLIIISLIQSSHQDYY-----------TPTKNNMNIIDSCWRSSSNWAKNRQAL 65
           S LL+ L +  SL+ +++  YY              +N MN +DSCWR  S+W  NR+ L
Sbjct: 7   SCLLVALFLCQSLVHAAYNGYYGYSPAAAPYPAEEPQNIMNPVDSCWRLKSDWDVNREDL 66

Query: 66  ANCAVGYGKDAIGGKFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLK 125
           A+CAVG+G   +GGK G IYVVT+P DN  NP  G+LRYGVIQ KPLW+ FAK MVI L 
Sbjct: 67  ADCAVGFGSSTLGGKKGNIYVVTNPYDNAQNPHPGSLRYGVIQAKPLWITFAKDMVITLA 126

Query: 126 NELMINSFKTIDGRGANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVG 185
           NELM+NS+KTIDGRGA VEIAYGPCIT+Q V++VI+HGISIHDCKP K+G VR + THVG
Sbjct: 127 NELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVIVHGISIHDCKPGKSGKVRSSPTHVG 186

Query: 186 KRRGSDGDAISIFASSHVWIDHCYLAQSTD------------------------------ 245
            R+GSDGDAI+IF SS+VWIDHCYLA  TD                              
Sbjct: 187 HRKGSDGDAITIFGSSNVWIDHCYLASCTDGLIDVIHASTAITISNNYFTQHDKVMLLGH 246

Query: 246 -------------------------------------ANNRYDEWKMYAIGGSANPTIFS 305
                                                ANNRYD+W MYAIGGSA+PTIFS
Sbjct: 247 NDNFVKDVKMKVTVAFNHFGPGLVERMPRVRRGYAHVANNRYDKWIMYAIGGSADPTIFS 306

Query: 306 EGNYFVAPKNSNAKQVTKREVNKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQS 315
           EGNYF+A   S +K+VTKREV  GWNNW+WR+S DVF NGA+FV SG+GS    Y+ AQ 
Sbjct: 307 EGNYFIASDKSYSKEVTKREVKGGWNNWRWRTSNDVFKNGAFFVPSGYGSIPLPYSSAQR 366

[Close]

BLAST of Cp4.1LG20g03400 vs. TAIR 10
Match: AT1G30350.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 323.6 bits (828), Expect = 1.8e-88
Identity = 176/374 (47.06%), Postives = 218/374 (58.29%), Query Frame = 0


Query: 9   LLTLLIIISLIQSSHQDYYTPTKNNMNIIDSCWRSSSNWAKNRQALANCAVGYGKDAIGG 68
           LL       L++++H    T  + ++N ID+CWR +  WA NRQALA+CAVGYGK AIGG
Sbjct: 10  LLLAAFASPLLETAHSYNVTAPRVSLNPIDACWRRNPKWATNRQALAHCAVGYGKAAIGG 69

Query: 69  KFGTIYVVTDPSDNPSNPKYGTLRYGVIQNKPLWVVFAKSMVIVLKNELMINSFKTIDGR 128
           K G IYVVT+PSDNP+ P  GTLRY V Q KPLW+ FA+ MVIVLK++LMINS+KTIDGR
Sbjct: 70  KNGPIYVVTNPSDNPTRPSPGTLRYAVSQPKPLWITFARDMVIVLKSQLMINSYKTIDGR 129

Query: 129 GANVEIAYGPCITVQGVSHVIIHGISIHDCKPAKAGLVRDTVTHVGKRRGSDGDAISIFA 188
           GA VEIA GPC+ ++ V HVIIHGISIHDCK                  G DGD I +F 
Sbjct: 130 GAKVEIANGPCLRIRQVKHVIIHGISIHDCK--------------ADPNGMDGDGIRVFQ 189

Query: 189 SSHVWIDHCYLAQSTD-------------------------------------------- 248
           S+HVWIDHC+L++  D                                            
Sbjct: 190 STHVWIDHCFLSRCHDGLIDVIVSSTAVTISNNYFTQHDKVMLLGHDDSYMGDKDMRVTI 249

Query: 249 -----------------------ANNRYDEWKMYAIGGSANPTIFSEGNYFVAPKNSNAK 308
                                  ANNRY++W+MYAIGGSANP IFSEGNYFVAP+  ++K
Sbjct: 250 AFNTFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSANPIIFSEGNYFVAPEKRSSK 309

Query: 309 QVTKREV-NKGWNNWKWRSSRDVFLNGAYFVQSGWGSCSPFYTRAQSFPVAPGSKVPALT 315
           QVTKR +       WKW +SRDVF+NGA+F   G     P Y   + F VA GS VP+LT
Sbjct: 310 QVTKRMMAGPDSKRWKWGTSRDVFMNGAFFGPPG-VIVRPLYKGGEGFRVAHGSLVPSLT 368

[Close]

BLAST of Cp4.1LG20g03400 vs. TAIR 10
Match: AT1G67750.1 (Pectate lyase family protein )

HSP 1 Score: 299.3 bits (765), Expect = 3.7e-81
Identity = 162/350 (46.29%), Postives = 198/350 (56.57%), Query Frame = 0


Query: 35  NIIDSCWRSSSNWAKNRQALANCAVGYGKDAIGGKFGTIYVVTDPSD-NPSNPKYGTLRY 94
           N ID CWR    W KNRQ LA+CA+G+GK AIGG+ G IYVVTD SD +  NPK GTLR+
Sbjct: 60  NPIDDCWRCDPKWEKNRQRLADCAIGFGKHAIGGRDGKIYVVTDSSDKDVVNPKPGTLRH 119

Query: 95  GVIQNKPLWVVFAKSMVIVLKNELMINSFKTIDGRGANVEIAYGPCITVQGVSHVIIHGI 154
            VIQ++PLW++FA+ MVI LK EL++NSFKTIDGRGA+V IA G CITVQ V+++IIHG+
Sbjct: 120 AVIQDEPLWIIFARDMVIKLKEELIMNSFKTIDGRGASVHIAGGACITVQYVTNIIIHGV 179

Query: 155 SIHDCKPAKAGLVRDTVTHVGKRRGSDGDAISIFASSHVWIDHCYLAQSTD--------- 214
           +IHDCK      VRD+ +H G R  SDGDA+SIF  SHVW+DHC L+   D         
Sbjct: 180 NIHDCKRKGNAYVRDSPSHYGWRTASDGDAVSIFGGSHVWVDHCSLSNCADGLIDAIHGS 239

Query: 215 ----------------------------------------------------------AN 274
                                                                      N
Sbjct: 240 TAITISNNYLSHHNKVMLLGHSDSYTRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVN 299

Query: 275 NRYDEWKMYAIGGSANPTIFSEGNYFVAPKNSNAKQVTKRE--VNKGWNNWKWRSSRDVF 315
           N Y  W+MYAIGGSA PTI S+GN F+AP +   K+VTK E      W  W WRS  D+F
Sbjct: 300 NDYTHWQMYAIGGSAAPTINSQGNRFLAPNDHVFKEVTKYEDAPRSKWKKWNWRSEGDLF 359

The following BLAST results are available for this feature:

BLAST of Cp4.1LG20g03400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65456	3.7e-110	52.97	Probable pectate lyase 16 OS=Arabidopsis thaliana OX=3702 GN=At4g22080 PE=2 SV=1	[more]
O65388	2.0e-108	51.97	Putative pectate lyase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g11920 PE=3 SV=2	[more]
O65457	1.1e-106	51.94	Putative pectate lyase 17 OS=Arabidopsis thaliana OX=3702 GN=At4g22090 PE=3 SV=1	[more]
Q9C8G4	2.6e-87	47.06	Probable pectate lyase 4 OS=Arabidopsis thaliana OX=3702 GN=At1g30350 PE=2 SV=1	[more]
Q93Z25	5.2e-80	44.73	Probable pectate lyase 22 OS=Arabidopsis thaliana OX=3702 GN=At5g63180 PE=2 SV=1	[more]

BLAST of Cp4.1LG20g03400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023520154.1	6.44e-223	82.41	putative pectate lyase 2 [Cucurbita pepo subsp. pepo]	[more]
KAG7019700.1	2.62e-222	82.15	putative pectate lyase 2, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022923701.1	2.15e-221	81.89	putative pectate lyase 2 [Cucurbita moschata]	[more]
KAG6584109.1	1.24e-220	81.63	putative pectate lyase 16, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023001281.1	1.69e-218	80.84	probable pectate lyase 16 [Cucurbita maxima]	[more]

BLAST of Cp4.1LG20g03400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E7G9	1.04e-221	81.89	Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111431331 PE=3 SV=1	[more]
A0A6J1KI65	8.17e-219	80.84	Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111495456 PE=3 SV=1	[more]
A0A0A0LU35	1.41e-196	71.92	Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_1G059200 PE=3 SV=1	[more]
A0A1S3B1W2	2.11e-191	70.60	Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103485080 PE=3 SV=1	[more]
A0A6J1C888	1.20e-188	69.37	Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111008879 PE=3 SV=1	[more]

BLAST of Cp4.1LG20g03400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G22080.1	2.6e-111	52.97	root hair specific 14	[more]
AT1G11920.1	1.4e-109	51.97	Pectin lyase-like superfamily protein	[more]
AT4G22090.1	7.8e-108	51.94	Pectin lyase-like superfamily protein	[more]
AT1G30350.1	1.8e-88	47.06	Pectin lyase-like superfamily protein	[more]
AT1G67750.1	3.7e-81	46.29	Pectate lyase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018082	AmbAllergen	PRINTS	PR00807	AMBALLERGEN	coord: 57..74 score: 56.61 coord: 180..201 score: 58.87 coord: 116..132 score: 72.83 coord: 80..105 score: 56.59
IPR002022	Pectate lyase	SMART	SM00656	amb_all	coord: 108..238 e-value: 1.4E-17 score: 74.4
IPR002022	Pectate lyase	PFAM	PF00544	Pectate_lyase_4	coord: 114..203 e-value: 3.5E-5 score: 23.5
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 206..307 e-value: 6.6E-22 score: 79.9 coord: 34..205 e-value: 4.2E-55 score: 189.1
None	No IPR available	PANTHER	PTHR31683:SF80	PECTATE LYASE 16-RELATED	coord: 205..312
None	No IPR available	PANTHER	PTHR31683:SF80	PECTATE LYASE 16-RELATED	coord: 13..204
IPR045032	Pectin lyase family	PANTHER	PTHR31683	PECTATE LYASE 18-RELATED	coord: 13..204
IPR045032	Pectin lyase family	PANTHER	PTHR31683	PECTATE LYASE 18-RELATED	coord: 205..312
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 29..308

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG20g03400.1	Cp4.1LG20g03400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0030570	pectate lyase activity