Cp4.1LG20g00270 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG20g00270
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionPectate lyase
LocationCp4.1LG20: 184730 .. 186118 (-)
RNA-Seq ExpressionCp4.1LG20g00270
SyntenyCp4.1LG20g00270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CACAGCAGCAACGATCAACCAAAAATGGGCGCCACCCAACTCTTTGTTCTGTCTCTTATTTCCCTTACTCTCCTCTCCGCAGTTTCTTATTCAGAAGGCAATGGGTTGAAGATGAACGCCATTGACAGATGCTGGAGATCGAACCCGTACTGGAGGAGAAACAGGCAACAACTGGGCGTTTGCTCGGTGGGTTTCGTTGGAAAAATGACCAACAACGTCGGAAGAAATCTCATACGCTACCAAGTCACCGACCCTAGCGACGACCCTATAAATCCACGCCCAGGTACACTCCGATACGGCGCCACAATGATGAAGTCCAAAGTCTGGATCACCTTCCAAAAAGACATGCACATCCTTCTCCACAAGCCGCTTCTCATAAGCAGCTACACAGCCATCGACGGGCGAGGGGCCAGCGTCCACATCTCCGGGAATGCATGCCTGGTGGTCTCCAAGGCCACCAACATCATTATCCACGGCCTTATCATTCACCACTGCAAGGCTCAGGGCGCAGGCCAAGTCATGGGCCCTAATTCCAAGTTGGTTTCACTCGGACACGTGGACGGCGATGCCATTCGTTTAGTTTCAGCCTCCAAAGTCTGGATTGACCACAACACACTGTACCGTTGCGAGGATGGGCTTCTCGACGTCACCAGAGGCTCCACTGACATCACCATTTCCAACAATTGGTTCCGCGACCAAGACAAGGTTATTCTTCTCGGCCACGAGGACGGCTATGTCCGAGACATGAACATGAGAGTCACTGTGGTCTATAACCATTTTGGACCCAACTGCAACCAAAGGATGCCCAGGTAAGGCTTTACAATGATATAATATTGTCTACTTTAAGCCTAAGTTTTTAAGCGACTTCTCAATAGTGTTTATTTTTTAATTATAAATTTACGTCATTCTCTGAATTAACATGGTATTAGATTAAAATCATTTGTTTTATAGGATCCGGCATGGGTATGCGCACGTGGCGAACAATTTGTACCAAGGATGGACGCAATACGCAATTGGAGGGAGCATGAATCCAAGCGTCAAGAGTGAAGCCAACCTGTTCATCGCATCAAAATCAAAACAGGCAATAATGACATAAACTAAAATGGAAATTTACAAAATATCGATTAGAGATTTGAATTTATTTTGTGTGAATAACAATGGCAGATTGTATGGAGCACCGGGAAAGTAGAGGCGGCAAAATGGAAGTTTCATTCCGTAAGAGACGTTTTTGAAAACGGAGCTTCCTTTGGACAGATAGGTGTGGGGCGAGGAGGCGTAAAGCCAAATTACAGCGCCCAGCAGCGGTTTCCAGTGGCTGATGCTAAGTGGGTGAGATCATTGACAAGCTCCTCGGGGGCTCTACGCTGCTCGTCAAAATCAAGATGTTGA

mRNA sequence

CACAGCAGCAACGATCAACCAAAAATGGGCGCCACCCAACTCTTTGTTCTGTCTCTTATTTCCCTTACTCTCCTCTCCGCAGTTTCTTATTCAGAAGGCAATGGGTTGAAGATGAACGCCATTGACAGATGCTGGAGATCGAACCCGTACTGGAGGAGAAACAGGCAACAACTGGGCGTTTGCTCGGTGGGTTTCGTTGGAAAAATGACCAACAACGTCGGAAGAAATCTCATACGCTACCAAGTCACCGACCCTAGCGACGACCCTATAAATCCACGCCCAGGTACACTCCGATACGGCGCCACAATGATGAAGTCCAAAGTCTGGATCACCTTCCAAAAAGACATGCACATCCTTCTCCACAAGCCGCTTCTCATAAGCAGCTACACAGCCATCGACGGGCGAGGGGCCAGCGTCCACATCTCCGGGAATGCATGCCTGGTGGTCTCCAAGGCCACCAACATCATTATCCACGGCCTTATCATTCACCACTGCAAGGCTCAGGGCGCAGGCCAAGTCATGGGCCCTAATTCCAAGTTGGTTTCACTCGGACACGTGGACGGCGATGCCATTCGTTTAGTTTCAGCCTCCAAAGTCTGGATTGACCACAACACACTGTACCGTTGCGAGGATGGGCTTCTCGACGTCACCAGAGGCTCCACTGACATCACCATTTCCAACAATTGGTTCCGCGACCAAGACAAGGTTATTCTTCTCGGCCACGAGGACGGCTATGTCCGAGACATGAACATGAGAGTCACTGTGGTCTATAACCATTTTGGACCCAACTGCAACCAAAGGATGCCCAGGATCCGGCATGGGTATGCGCACGTGGCGAACAATTTGTACCAAGGATGGACGCAATACGCAATTGGAGGGAGCATGAATCCAAGCATTGTATGGAGCACCGGGAAAGTAGAGGCGGCAAAATGGAAGTTTCATTCCGTAAGAGACGTTTTTGAAAACGGAGCTTCCTTTGGACAGATAGGTGTGGGGCGAGGAGGCGTAAAGCCAAATTACAGCGCCCAGCAGCGGTTTCCAGTGGCTGATGCTAAGTGGGTGAGATCATTGACAAGCTCCTCGGGGGCTCTACGCTGCTCGTCAAAATCAAGATGTTGA

Coding sequence (CDS)

CACAGCAGCAACGATCAACCAAAAATGGGCGCCACCCAACTCTTTGTTCTGTCTCTTATTTCCCTTACTCTCCTCTCCGCAGTTTCTTATTCAGAAGGCAATGGGTTGAAGATGAACGCCATTGACAGATGCTGGAGATCGAACCCGTACTGGAGGAGAAACAGGCAACAACTGGGCGTTTGCTCGGTGGGTTTCGTTGGAAAAATGACCAACAACGTCGGAAGAAATCTCATACGCTACCAAGTCACCGACCCTAGCGACGACCCTATAAATCCACGCCCAGGTACACTCCGATACGGCGCCACAATGATGAAGTCCAAAGTCTGGATCACCTTCCAAAAAGACATGCACATCCTTCTCCACAAGCCGCTTCTCATAAGCAGCTACACAGCCATCGACGGGCGAGGGGCCAGCGTCCACATCTCCGGGAATGCATGCCTGGTGGTCTCCAAGGCCACCAACATCATTATCCACGGCCTTATCATTCACCACTGCAAGGCTCAGGGCGCAGGCCAAGTCATGGGCCCTAATTCCAAGTTGGTTTCACTCGGACACGTGGACGGCGATGCCATTCGTTTAGTTTCAGCCTCCAAAGTCTGGATTGACCACAACACACTGTACCGTTGCGAGGATGGGCTTCTCGACGTCACCAGAGGCTCCACTGACATCACCATTTCCAACAATTGGTTCCGCGACCAAGACAAGGTTATTCTTCTCGGCCACGAGGACGGCTATGTCCGAGACATGAACATGAGAGTCACTGTGGTCTATAACCATTTTGGACCCAACTGCAACCAAAGGATGCCCAGGATCCGGCATGGGTATGCGCACGTGGCGAACAATTTGTACCAAGGATGGACGCAATACGCAATTGGAGGGAGCATGAATCCAAGCATTGTATGGAGCACCGGGAAAGTAGAGGCGGCAAAATGGAAGTTTCATTCCGTAAGAGACGTTTTTGAAAACGGAGCTTCCTTTGGACAGATAGGTGTGGGGCGAGGAGGCGTAAAGCCAAATTACAGCGCCCAGCAGCGGTTTCCAGTGGCTGATGCTAAGTGGGTGAGATCATTGACAAGCTCCTCGGGGGCTCTACGCTGCTCGTCAAAATCAAGATGTTGA

Protein sequence

HSSNDQPKMGATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNVGRNLIRYQVTDPSDDPINPRPGTLRYGATMMKSKVWITFQKDMHILLHKPLLISSYTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRDMNMRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSIVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKWVRSLTSSSGALRCSSKSRC
Homology
BLAST of Cp4.1LG20g00270 vs. ExPASy Swiss-Prot
Match: O65388 (Putative pectate lyase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g11920 PE=3 SV=2)

HSP 1 Score: 318.5 bits (815), Expect = 9.7e-86
Identity = 177/390 (45.38%), Postives = 230/390 (58.97%), Query Frame = 0

Query: 9   MGATQLFVLSLISLTLLSAV---SYSEG---NGLKMNAIDRCWRSNPYWRRNRQQLGVCS 68
           M +  L ++SL+     S+V   +YS G     L  N ID CWR NPYW  NR+ L  C+
Sbjct: 1   MASLFLTIISLLFAAFSSSVVEAAYSNGYTIPKLLPNPIDSCWRRNPYWASNRRALADCA 60

Query: 69  VGFVGKMTNNVGRNLIRYQVTDPSDDPINPRPGTLRYGATMMKSKVWITFQKDMHILLHK 128
           VGF GK     G+    Y VT+PSDDP NPRPGTLRY A +    +WITF +DM I+L  
Sbjct: 61  VGF-GKSAVG-GKYGSIYVVTNPSDDPENPRPGTLRY-AVIQSKPLWITFARDMVIVLRN 120

Query: 129 PLLISSYTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVS 188
            L+++SY  IDGRGA V I+   C+ +   +++IIHG+ IH CK   +G+V    + + S
Sbjct: 121 ELIMNSYKTIDGRGAKVEIAYGPCITIQHVSHVIIHGISIHDCKPGKSGRVRSSPTHVGS 180

Query: 189 LGHVDGDAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHE 248
               DGDAI +  +S +WIDH    RC+DGL+DV   ST +TISNN+F   DKV+LLGH 
Sbjct: 181 RKGSDGDAIAIFDSSHIWIDHCFFSRCQDGLIDVLHASTAVTISNNYFTQHDKVMLLGHN 240

Query: 249 DGYVRDMNMRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSIVWS 308
           D  V D  MRVT+ +NHFGP   +RMPR+R GYAHVANN Y+ W  YAIGGS +P+I +S
Sbjct: 241 DNNVEDKIMRVTIAFNHFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSADPTI-FS 300

Query: 309 TGKVEAA--------------------KWKFHSVRDVFENGASFGQIGVGRGGVKPNYSA 368
            G    A                    +WK+ + +DVF+NGA F  +  G G V P Y  
Sbjct: 301 EGNYFVASDDPSKKQVTKRIDSGYDWKRWKWRTSKDVFKNGAYF--VPSGYGTVTPLYGR 360

Query: 369 QQRFPVADAKWVRSLTSSSGALRCSSKSRC 373
            +RFPV+    V  LTSS+G L C S   C
Sbjct: 361 AERFPVSHGSLVPLLTSSAGPLHCYSGRIC 384

BLAST of Cp4.1LG20g00270 vs. ExPASy Swiss-Prot
Match: Q9C8G4 (Probable pectate lyase 4 OS=Arabidopsis thaliana OX=3702 GN=At1g30350 PE=2 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 1.7e-82
Identity = 173/374 (46.26%), Postives = 222/374 (59.36%), Query Frame = 0

Query: 14  LFVLSLISLTLLSAVSYS-EGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNN 73
           L + +  S  L +A SY+     + +N ID CWR NP W  NRQ L  C+VG+ GK    
Sbjct: 10  LLLAAFASPLLETAHSYNVTAPRVSLNPIDACWRRNPKWATNRQALAHCAVGY-GKAAIG 69

Query: 74  VGRNLIRYQVTDPSDDPINPRPGTLRYGATMMKSKVWITFQKDMHILLHKPLLISSYTAI 133
            G+N   Y VT+PSD+P  P PGTLRY  +  K  +WITF +DM I+L   L+I+SY  I
Sbjct: 70  -GKNGPIYVVTNPSDNPTRPSPGTLRYAVSQPK-PLWITFARDMVIVLKSQLMINSYKTI 129

Query: 134 DGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDAIR 193
           DGRGA V I+   CL + +  ++IIHG+ IH CKA        PN        +DGD IR
Sbjct: 130 DGRGAKVEIANGPCLRIRQVKHVIIHGISIHDCKAD-------PNG-------MDGDGIR 189

Query: 194 LVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRDMNMR 253
           +  ++ VWIDH  L RC DGL+DV   ST +TISNN+F   DKV+LLGH+D Y+ D +MR
Sbjct: 190 VFQSTHVWIDHCFLSRCHDGLIDVIVSSTAVTISNNYFTQHDKVMLLGHDDSYMGDKDMR 249

Query: 254 VTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSIVWSTGKV------ 313
           VT+ +N FGP   +RMPR+R GYAHVANN Y+ W  YAIGGS NP I++S G        
Sbjct: 250 VTIAFNTFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSANP-IIFSEGNYFVAPEK 309

Query: 314 --------------EAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAK 367
                         ++ +WK+ + RDVF NGA FG  GV    V+P Y   + F VA   
Sbjct: 310 RSSKQVTKRMMAGPDSKRWKWGTSRDVFMNGAFFGPPGV---IVRPLYKGGEGFRVAHGS 362

BLAST of Cp4.1LG20g00270 vs. ExPASy Swiss-Prot
Match: O65456 (Probable pectate lyase 16 OS=Arabidopsis thaliana OX=3702 GN=At4g22080 PE=2 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 3.6e-80
Identity = 158/354 (44.63%), Postives = 203/354 (57.34%), Query Frame = 0

Query: 38  MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNVGRNLIRYQVTDPSDDPINPRPGTL 97
           MN +D CWR    W  NR+ L  C VGF          NL  Y VT+P D+  NP+PG+L
Sbjct: 46  MNPVDSCWRLKSDWAANRKDLADCVVGFGSSTLGGKKGNL--YVVTNPYDNAQNPQPGSL 105

Query: 98  RYGATMMKSKVWITFQKDMHILLHKPLLISSYTAIDGRGASVHISGNACLVVSKATNIII 157
           RYG    K  +WITF KDM I L   L+++SY  IDGRGA V I+   C+ +   TN+I+
Sbjct: 106 RYGVIQAK-PLWITFAKDMVITLENELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVIV 165

Query: 158 HGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDAIRLVSASKVWIDHNTLYRCEDGLLDVT 217
           HG+ IH CK    G V    + +      DGDAI +  +S +WIDH  L  C DGL+DV 
Sbjct: 166 HGISIHDCKPGKYGMVRSSPTHVGHRKGSDGDAIAIFGSSNIWIDHCYLASCTDGLIDVI 225

Query: 218 RGSTDITISNNWFRDQDKVILLGHEDGYVRDMNMRVTVVYNHFGPNCNQRMPRIRHGYAH 277
             ST ITISNN+F   DKV+LLGH D +V+D+ M+VTV +NHFGP   +RMPR+R GYAH
Sbjct: 226 HASTGITISNNYFTQHDKVMLLGHNDDFVQDVKMKVTVAFNHFGPGLVERMPRVRRGYAH 285

Query: 278 VANNLYQGWTQYAIGGSMNPSIVWSTGKVEAA-------------------KWKFHSVRD 337
           VANN Y  W  YAIGGS +P+I +S G    A                    W++ + +D
Sbjct: 286 VANNRYDKWIMYAIGGSADPTI-FSEGNYFIASDKSNSKEVTKREVKGGWNNWRWRTSKD 345

Query: 338 VFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKWVRSLTSSSGALRCSSKSRC 373
           VF+NGA F  +  G G +   YS+ QRF VA    V SLT+ +G L C+    C
Sbjct: 346 VFKNGAYF--VPSGYGSISLPYSSAQRFTVAPGNLVPSLTADAGPLNCNRNGPC 393

BLAST of Cp4.1LG20g00270 vs. ExPASy Swiss-Prot
Match: O65457 (Putative pectate lyase 17 OS=Arabidopsis thaliana OX=3702 GN=At4g22090 PE=3 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 4.7e-80
Identity = 158/354 (44.63%), Postives = 204/354 (57.63%), Query Frame = 0

Query: 38  MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNVGRNLIRYQVTDPSDDPINPRPGTL 97
           MN +D CWR    W  NR+ L  C+VGF          N+  Y VT+P D+  NP PG+L
Sbjct: 46  MNPVDSCWRLKSDWDVNREDLADCAVGFGSSTLGGKKGNI--YVVTNPYDNAQNPHPGSL 105

Query: 98  RYGATMMKSKVWITFQKDMHILLHKPLLISSYTAIDGRGASVHISGNACLVVSKATNIII 157
           RYG    K  +WITF KDM I L   L+++SY  IDGRGA V I+   C+ +   TN+I+
Sbjct: 106 RYGVIQAK-PLWITFAKDMVITLANELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVIV 165

Query: 158 HGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDAIRLVSASKVWIDHNTLYRCEDGLLDVT 217
           HG+ IH CK   +G+V    + +      DGDAI +  +S VWIDH  L  C DGL+DV 
Sbjct: 166 HGISIHDCKPGKSGKVRSSPTHVGHRKGSDGDAITIFGSSNVWIDHCYLASCTDGLIDVI 225

Query: 218 RGSTDITISNNWFRDQDKVILLGHEDGYVRDMNMRVTVVYNHFGPNCNQRMPRIRHGYAH 277
             ST ITISNN+F   DKV+LLGH D +V+D+ M+VTV +NHFGP   +RMPR+R GYAH
Sbjct: 226 HASTAITISNNYFTQHDKVMLLGHNDNFVKDVKMKVTVAFNHFGPGLVERMPRVRRGYAH 285

Query: 278 VANNLYQGWTQYAIGGSMNPSIVWSTGKVEAA-------------------KWKFHSVRD 337
           VANN Y  W  YAIGGS +P+I +S G    A                    W++ +  D
Sbjct: 286 VANNRYDKWIMYAIGGSADPTI-FSEGNYFIASDKSYSKEVTKREVKGGWNNWRWRTSND 345

Query: 338 VFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKWVRSLTSSSGALRCSSKSRC 373
           VF+NGA F  +  G G +   YS+ QRF VA    V SLT+ +G L C+    C
Sbjct: 346 VFKNGAFF--VPSGYGSIPLPYSSAQRFTVAPGNLVPSLTADAGPLNCNRNGPC 393

BLAST of Cp4.1LG20g00270 vs. ExPASy Swiss-Prot
Match: Q9LRM5 (Probable pectate lyase 9 OS=Arabidopsis thaliana OX=3702 GN=At3g24230 PE=2 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 2.3e-79
Identity = 163/364 (44.78%), Postives = 204/364 (56.04%), Query Frame = 0

Query: 30  YSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNVGRNLIRYQVTDPSDDP 89
           +S GNG   N ID CWR +  W +NR+ L  C +GF  K     GRN   Y VTD SDD 
Sbjct: 98  FSCGNG---NLIDDCWRCDRNWNKNRKHLADCGMGFGSKAFG--GRNGSYYVVTDHSDDD 157

Query: 90  -INPRPGTLRYGATMMKSKVWITFQKDMHILLHKPLLISSYTAIDGRGASVHISGNACLV 149
            +NP+PGTLR+ A +    +WI F++DM I L + L+++S+  ID RGA+VHI+  AC+ 
Sbjct: 158 VVNPKPGTLRH-AVIQVEPLWIIFKRDMVIKLKQELIMNSFKTIDARGANVHIANGACIT 217

Query: 150 VSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDAIRLVSASKVWIDHNTLYR 209
           +   TN+I+HGL IH CK  G   V    S+    G  DGDAI +  +S +WIDHN+L  
Sbjct: 218 IQNITNVIVHGLHIHDCKRTGNVTVRSSPSQAGFRGTADGDAINIFGSSHIWIDHNSLSN 277

Query: 210 CEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRDMNMRVTVVYNHFGPNCNQRM 269
           C DGL+DV  GST ITISNN F   D+V+LLGH D Y RD  M+VTV YNHFG    QRM
Sbjct: 278 CTDGLVDVVNGSTAITISNNHFTHHDEVMLLGHNDSYTRDKMMQVTVAYNHFGEGLIQRM 337

Query: 270 PRIRHGYAHVANNLYQGWTQYAIGGSMNPSIVWSTGKVEAAK------------------ 329
           PR RHGY HV NN Y  W  YAIGGS NP+I     +  A K                  
Sbjct: 338 PRCRHGYFHVVNNDYTHWKMYAIGGSANPTINSQGNRFAAPKNHSAKEVTKRLDTKGNEW 397

Query: 330 --WKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKWVRSLTSSSGALRCSS 373
             W + S +D+  NGA F   G G  G     S     P   A  V ++T+S+GAL C  
Sbjct: 398 MEWNWRSEKDLLVNGAFFTPSGEGASGDSQTLS----LPAKPASMVDAITASAGALSCRR 451

BLAST of Cp4.1LG20g00270 vs. NCBI nr
Match: XP_023520094.1 (putative pectate lyase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 741 bits (1913), Expect = 1.24e-269
Identity = 364/379 (96.04%), Postives = 364/379 (96.04%), Query Frame = 0

Query: 9   MGATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGK 68
           MGATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGK
Sbjct: 1   MGATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGK 60

Query: 69  MTNNVGRNLIRYQVTDPSDDPINPRPGTLRYGATMMKSKVWITFQKDMHILLHKPLLISS 128
           MTNNVGRNLIRYQVTDPSDDPINPRPGTLRYGATMMKSKVWITFQKDMHILLHKPLLISS
Sbjct: 61  MTNNVGRNLIRYQVTDPSDDPINPRPGTLRYGATMMKSKVWITFQKDMHILLHKPLLISS 120

Query: 129 YTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDG 188
           YTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDG
Sbjct: 121 YTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDG 180

Query: 189 DAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRD 248
           DAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRD
Sbjct: 181 DAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRD 240

Query: 249 MNMRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPS---------- 308
           MNMRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPS          
Sbjct: 241 MNMRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIA 300

Query: 309 -----IVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKW 368
                IVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKW
Sbjct: 301 SKSKQIVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKW 360

Query: 369 VRSLTSSSGALRCSSKSRC 372
           VRSLTSSSGALRCSSKSRC
Sbjct: 361 VRSLTSSSGALRCSSKSRC 379

BLAST of Cp4.1LG20g00270 vs. NCBI nr
Match: KAG7020030.1 (putative pectate lyase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 737 bits (1903), Expect = 4.16e-268
Identity = 362/379 (95.51%), Postives = 363/379 (95.78%), Query Frame = 0

Query: 9   MGATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGK 68
           MGATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGK
Sbjct: 1   MGATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGK 60

Query: 69  MTNNVGRNLIRYQVTDPSDDPINPRPGTLRYGATMMKSKVWITFQKDMHILLHKPLLISS 128
           MTNNVGRNLIRYQVTDPSDDPINPRPGTLRYGATM+KSKVWITFQKDMHILLHKPLLISS
Sbjct: 61  MTNNVGRNLIRYQVTDPSDDPINPRPGTLRYGATMIKSKVWITFQKDMHILLHKPLLISS 120

Query: 129 YTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDG 188
           YTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDG
Sbjct: 121 YTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDG 180

Query: 189 DAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRD 248
           DAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRD
Sbjct: 181 DAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRD 240

Query: 249 MNMRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPS---------- 308
            NMRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPS          
Sbjct: 241 KNMRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIA 300

Query: 309 -----IVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKW 368
                IVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKW
Sbjct: 301 SKSKQIVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKW 360

Query: 369 VRSLTSSSGALRCSSKSRC 372
           VRSLTSSSGALRCSSKSRC
Sbjct: 361 VRSLTSSSGALRCSSKSRC 379

BLAST of Cp4.1LG20g00270 vs. NCBI nr
Match: XP_022923821.1 (putative pectate lyase 2 [Cucurbita moschata])

HSP 1 Score: 737 bits (1902), Expect = 5.90e-268
Identity = 361/379 (95.25%), Postives = 363/379 (95.78%), Query Frame = 0

Query: 9   MGATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGK 68
           MGATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGK
Sbjct: 1   MGATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGK 60

Query: 69  MTNNVGRNLIRYQVTDPSDDPINPRPGTLRYGATMMKSKVWITFQKDMHILLHKPLLISS 128
           MTNN+GRNLIRYQVTDPSDDPINPRPGTLRYGATM+KSKVWITFQKDMHILLHKPLLISS
Sbjct: 61  MTNNIGRNLIRYQVTDPSDDPINPRPGTLRYGATMIKSKVWITFQKDMHILLHKPLLISS 120

Query: 129 YTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDG 188
           YTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDG
Sbjct: 121 YTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDG 180

Query: 189 DAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRD 248
           DAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRD
Sbjct: 181 DAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRD 240

Query: 249 MNMRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPS---------- 308
            NMRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPS          
Sbjct: 241 KNMRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIA 300

Query: 309 -----IVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKW 368
                IVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKW
Sbjct: 301 SKSKQIVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKW 360

Query: 369 VRSLTSSSGALRCSSKSRC 372
           VRSLTSSSGALRCSSKSRC
Sbjct: 361 VRSLTSSSGALRCSSKSRC 379

BLAST of Cp4.1LG20g00270 vs. NCBI nr
Match: XP_023001119.1 (putative pectate lyase 2 [Cucurbita maxima])

HSP 1 Score: 728 bits (1879), Expect = 1.89e-264
Identity = 357/379 (94.20%), Postives = 360/379 (94.99%), Query Frame = 0

Query: 9   MGATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGK 68
           MGATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGK
Sbjct: 1   MGATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGK 60

Query: 69  MTNNVGRNLIRYQVTDPSDDPINPRPGTLRYGATMMKSKVWITFQKDMHILLHKPLLISS 128
           MTNN+GRNLIRYQVTDPSDDPINPRPGTLRYGATM+KSKVWITFQK MHILLHKPLLISS
Sbjct: 61  MTNNIGRNLIRYQVTDPSDDPINPRPGTLRYGATMIKSKVWITFQKGMHILLHKPLLISS 120

Query: 129 YTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDG 188
           YTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDG
Sbjct: 121 YTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDG 180

Query: 189 DAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRD 248
           DAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRD
Sbjct: 181 DAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRD 240

Query: 249 MNMRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPS---------- 308
            NMRVTV+YNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPS          
Sbjct: 241 KNMRVTVIYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIA 300

Query: 309 -----IVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKW 368
                IVWSTGKVEAAKWKFHSV DVFENGASF QIGVGRGGVKPNYSAQQRFPVADAKW
Sbjct: 301 SKLKQIVWSTGKVEAAKWKFHSVGDVFENGASFEQIGVGRGGVKPNYSAQQRFPVADAKW 360

Query: 369 VRSLTSSSGALRCSSKSRC 372
           VRSLTSSSGALRCSSKSRC
Sbjct: 361 VRSLTSSSGALRCSSKSRC 379

BLAST of Cp4.1LG20g00270 vs. NCBI nr
Match: KAG6584442.1 (putative pectate lyase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 717 bits (1850), Expect = 8.27e-256
Identity = 344/347 (99.14%), Postives = 345/347 (99.42%), Query Frame = 0

Query: 9   MGATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGK 68
           MGATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGK
Sbjct: 1   MGATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGK 60

Query: 69  MTNNVGRNLIRYQVTDPSDDPINPRPGTLRYGATMMKSKVWITFQKDMHILLHKPLLISS 128
           MTNNVGRNLIRYQVTDPSDDPINPRPGTLRYGATM+KSKVWITFQKDMHILLHKPLLISS
Sbjct: 61  MTNNVGRNLIRYQVTDPSDDPINPRPGTLRYGATMIKSKVWITFQKDMHILLHKPLLISS 120

Query: 129 YTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDG 188
           YTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDG
Sbjct: 121 YTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDG 180

Query: 189 DAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRD 248
           DAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRD
Sbjct: 181 DAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRD 240

Query: 249 MNMRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSIVWSTGKVEA 308
            NMRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSIVWSTGKVEA
Sbjct: 241 KNMRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSIVWSTGKVEA 300

Query: 309 AKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKWVR 355
           AKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKW R
Sbjct: 301 AKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKWAR 347

BLAST of Cp4.1LG20g00270 vs. ExPASy TrEMBL
Match: A0A6J1E7R9 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111431420 PE=3 SV=1)

HSP 1 Score: 737 bits (1902), Expect = 2.86e-268
Identity = 361/379 (95.25%), Postives = 363/379 (95.78%), Query Frame = 0

Query: 9   MGATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGK 68
           MGATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGK
Sbjct: 1   MGATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGK 60

Query: 69  MTNNVGRNLIRYQVTDPSDDPINPRPGTLRYGATMMKSKVWITFQKDMHILLHKPLLISS 128
           MTNN+GRNLIRYQVTDPSDDPINPRPGTLRYGATM+KSKVWITFQKDMHILLHKPLLISS
Sbjct: 61  MTNNIGRNLIRYQVTDPSDDPINPRPGTLRYGATMIKSKVWITFQKDMHILLHKPLLISS 120

Query: 129 YTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDG 188
           YTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDG
Sbjct: 121 YTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDG 180

Query: 189 DAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRD 248
           DAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRD
Sbjct: 181 DAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRD 240

Query: 249 MNMRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPS---------- 308
            NMRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPS          
Sbjct: 241 KNMRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIA 300

Query: 309 -----IVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKW 368
                IVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKW
Sbjct: 301 SKSKQIVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKW 360

Query: 369 VRSLTSSSGALRCSSKSRC 372
           VRSLTSSSGALRCSSKSRC
Sbjct: 361 VRSLTSSSGALRCSSKSRC 379

BLAST of Cp4.1LG20g00270 vs. ExPASy TrEMBL
Match: A0A6J1KLU6 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111495358 PE=3 SV=1)

HSP 1 Score: 728 bits (1879), Expect = 9.16e-265
Identity = 357/379 (94.20%), Postives = 360/379 (94.99%), Query Frame = 0

Query: 9   MGATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGK 68
           MGATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGK
Sbjct: 1   MGATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGK 60

Query: 69  MTNNVGRNLIRYQVTDPSDDPINPRPGTLRYGATMMKSKVWITFQKDMHILLHKPLLISS 128
           MTNN+GRNLIRYQVTDPSDDPINPRPGTLRYGATM+KSKVWITFQK MHILLHKPLLISS
Sbjct: 61  MTNNIGRNLIRYQVTDPSDDPINPRPGTLRYGATMIKSKVWITFQKGMHILLHKPLLISS 120

Query: 129 YTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDG 188
           YTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDG
Sbjct: 121 YTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDG 180

Query: 189 DAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRD 248
           DAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRD
Sbjct: 181 DAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRD 240

Query: 249 MNMRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPS---------- 308
            NMRVTV+YNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPS          
Sbjct: 241 KNMRVTVIYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIA 300

Query: 309 -----IVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKW 368
                IVWSTGKVEAAKWKFHSV DVFENGASF QIGVGRGGVKPNYSAQQRFPVADAKW
Sbjct: 301 SKLKQIVWSTGKVEAAKWKFHSVGDVFENGASFEQIGVGRGGVKPNYSAQQRFPVADAKW 360

Query: 369 VRSLTSSSGALRCSSKSRC 372
           VRSLTSSSGALRCSSKSRC
Sbjct: 361 VRSLTSSSGALRCSSKSRC 379

BLAST of Cp4.1LG20g00270 vs. ExPASy TrEMBL
Match: A0A1S3AY18 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103483978 PE=3 SV=1)

HSP 1 Score: 644 bits (1661), Expect = 1.69e-231
Identity = 309/377 (81.96%), Postives = 336/377 (89.12%), Query Frame = 0

Query: 13  QLFVLSLISLTLL--SAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMT 72
           QL VLSLIS   L  + VS SE +GL MNAIDRCWRSNPYWRRNRQQL  CSVGF GKMT
Sbjct: 6   QLSVLSLISFIFLISNVVSSSEAHGLSMNAIDRCWRSNPYWRRNRQQLATCSVGFAGKMT 65

Query: 73  NNVGRNLIRYQVTDPSDDPINPRPGTLRYGATMMKSKVWITFQKDMHILLHKPLLISSYT 132
           NN+GRNLI YQVTDPSDDPINPRPGTLRYGATM+K KVWITFQKDMHI+L KPLLISS+T
Sbjct: 66  NNMGRNLIHYQVTDPSDDPINPRPGTLRYGATMIKHKVWITFQKDMHIVLQKPLLISSFT 125

Query: 133 AIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDA 192
           AIDGRGA+VHI+GNACL+V +ATNIIIHGLIIHHC++Q AG+VMGPNSK++SLGHVDGDA
Sbjct: 126 AIDGRGATVHITGNACLMVLRATNIIIHGLIIHHCRSQAAGEVMGPNSKVISLGHVDGDA 185

Query: 193 IRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRDMN 252
           IRLV+ASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGH+DGYVRD N
Sbjct: 186 IRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGYVRDRN 245

Query: 253 MRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSI----------- 312
           M+VTVVYNHFGPNCNQRMPRIR+GYAHVANNLYQGWTQYAIGGSMNPS+           
Sbjct: 246 MKVTVVYNHFGPNCNQRMPRIRYGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIASK 305

Query: 313 ----VWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKWVR 372
               +WSTG VE AKWKFHSVRDVFENGA F +IGVGRG VKPNY+ QQRFPVADAKW+R
Sbjct: 306 SKQVIWSTGNVEEAKWKFHSVRDVFENGALFTEIGVGRGRVKPNYNPQQRFPVADAKWIR 365

BLAST of Cp4.1LG20g00270 vs. ExPASy TrEMBL
Match: A0A0A0LPZ1 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_1G031910 PE=3 SV=1)

HSP 1 Score: 644 bits (1661), Expect = 1.82e-231
Identity = 306/377 (81.17%), Postives = 340/377 (90.19%), Query Frame = 0

Query: 13  QLFVLSLISLTLL--SAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMT 72
           QL VLSLIS  LL  +AVSYSE +GL MNAIDRCWRSNPYWR+NRQQL  CSVGF GKMT
Sbjct: 8   QLSVLSLISFILLISTAVSYSEAHGLNMNAIDRCWRSNPYWRKNRQQLATCSVGFAGKMT 67

Query: 73  NNVGRNLIRYQVTDPSDDPINPRPGTLRYGATMMKSKVWITFQKDMHILLHKPLLISSYT 132
           NN+GRNL+ YQVTDPSDDPINPRPGTLRYGATM+K KVWITF+KDMHI+L KPLL+SS+T
Sbjct: 68  NNMGRNLMHYQVTDPSDDPINPRPGTLRYGATMIKHKVWITFKKDMHIVLQKPLLVSSFT 127

Query: 133 AIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDA 192
           AIDGRG++VHI+GNACL+V +ATNIIIHGLIIHHCK+Q AGQVMGPN+K++SLG+VDGDA
Sbjct: 128 AIDGRGSTVHITGNACLMVVRATNIIIHGLIIHHCKSQAAGQVMGPNAKVISLGNVDGDA 187

Query: 193 IRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRDMN 252
           IRLV+ASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGH+DGYVRD +
Sbjct: 188 IRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGYVRDRS 247

Query: 253 MRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSI----------- 312
           MRVTV+YNHFGPNCNQRMPRIR+GYAHVANNLYQGWTQYAIGGSMNPS+           
Sbjct: 248 MRVTVLYNHFGPNCNQRMPRIRYGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIASK 307

Query: 313 ----VWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKWVR 372
               +WSTGKVE AKWKFHSVRD FENGASF +IGVG+G VKPNY+ QQRFPVADAKW+R
Sbjct: 308 SKQVIWSTGKVEEAKWKFHSVRDAFENGASFAEIGVGKGRVKPNYNPQQRFPVADAKWIR 367

BLAST of Cp4.1LG20g00270 vs. ExPASy TrEMBL
Match: A0A6J1KAY1 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111492658 PE=3 SV=1)

HSP 1 Score: 619 bits (1595), Expect = 6.29e-222
Identity = 288/352 (81.82%), Postives = 319/352 (90.62%), Query Frame = 0

Query: 36  LKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNVGRNLIRYQVTDPSDDPINPRPG 95
           + MNAIDRCWRSNPYWR+NRQ+L +CS+GFVGKMTNN+GRNL+ YQVTDP+DDP+ PRPG
Sbjct: 1   MNMNAIDRCWRSNPYWRKNRQKLALCSIGFVGKMTNNIGRNLVHYQVTDPTDDPLTPRPG 60

Query: 96  TLRYGATMMKSKVWITFQKDMHILLHKPLLISSYTAIDGRGASVHISGNACLVVSKATNI 155
           TLRYGATM+K KVWITFQKDMHI+L+KPLLISSYTAIDGRGA+VHI+GNACL+VS+ATN+
Sbjct: 61  TLRYGATMIKPKVWITFQKDMHIVLNKPLLISSYTAIDGRGATVHITGNACLMVSRATNV 120

Query: 156 IIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDAIRLVSASKVWIDHNTLYRCEDGLLD 215
           IIHGLIIHHCKAQ AG+VMGP SK+VSLGHVDGDAIRLVSASK+WIDHNTLYRCEDGL+D
Sbjct: 121 IIHGLIIHHCKAQAAGRVMGPGSKIVSLGHVDGDAIRLVSASKIWIDHNTLYRCEDGLID 180

Query: 216 VTRGSTDITISNNWFRDQDKVILLGHEDGYVRDMNMRVTVVYNHFGPNCNQRMPRIRHGY 275
           VTRGSTDITISNNW RDQDKVILLGH+D Y+ D NM+VT+VYNHFGPNCNQRMPRIR+GY
Sbjct: 181 VTRGSTDITISNNWLRDQDKVILLGHDDDYIWDRNMKVTLVYNHFGPNCNQRMPRIRYGY 240

Query: 276 AHVANNLYQGWTQYAIGGSMNPS---------------IVWSTGKVEAAKWKFHSVRDVF 335
           AHVANNLYQGWTQYAIGGSMNPS               I+WSTGKVE AKW FHSVRDVF
Sbjct: 241 AHVANNLYQGWTQYAIGGSMNPSVKSEANLFMASKSKQIIWSTGKVENAKWNFHSVRDVF 300

Query: 336 ENGASFGQIGVGRGGVKPNYSAQQRFPVADAKWVRSLTSSSGALRCSSKSRC 372
           ENGASF QIG GRGGVKP+Y+A QRFPV DAKW+RSLTSSSGALRCS +SRC
Sbjct: 301 ENGASFAQIGAGRGGVKPHYNALQRFPVVDAKWLRSLTSSSGALRCSPRSRC 352

BLAST of Cp4.1LG20g00270 vs. TAIR 10
Match: AT5G09280.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 394.0 bits (1011), Expect = 1.3e-109
Identity = 174/231 (75.32%), Postives = 208/231 (90.04%), Query Frame = 0

Query: 69  MTNNVGRNLIRYQVTDPSDDPINPRPGTLRYGATMMKSKVWITFQKDMHILLHKPLLISS 128
           MT N+G+ + +Y+VTDPSDDP+NP+PGTLRYGAT++K K WITF+++M I LHKPLLISS
Sbjct: 1   MTGNIGKGVTQYKVTDPSDDPLNPKPGTLRYGATLVKGKKWITFKRNMKIKLHKPLLISS 60

Query: 129 YTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDG 188
           +TA+DGRGASVHISG ACL+V +AT++IIHGL IH CKA     VMGP+SK++ LGH+DG
Sbjct: 61  FTALDGRGASVHISGPACLIVYRATDVIIHGLKIHDCKAHPPSSVMGPDSKIIQLGHMDG 120

Query: 189 DAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRD 248
           DAIRLV+A KVWIDHNTLY CEDGLLDVT GSTD+T+SNNWFR+QDKV+LLGH+DGYV+D
Sbjct: 121 DAIRLVTAKKVWIDHNTLYDCEDGLLDVTLGSTDVTVSNNWFRNQDKVMLLGHDDGYVKD 180

Query: 249 MNMRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSI 300
            +MRVTVV+NHFGPNCNQRMPR+RHGYAHVANN YQGWTQYAIGGSM+P +
Sbjct: 181 KDMRVTVVFNHFGPNCNQRMPRVRHGYAHVANNYYQGWTQYAIGGSMSPRV 231

BLAST of Cp4.1LG20g00270 vs. TAIR 10
Match: AT1G11920.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 318.5 bits (815), Expect = 6.9e-87
Identity = 177/390 (45.38%), Postives = 230/390 (58.97%), Query Frame = 0

Query: 9   MGATQLFVLSLISLTLLSAV---SYSEG---NGLKMNAIDRCWRSNPYWRRNRQQLGVCS 68
           M +  L ++SL+     S+V   +YS G     L  N ID CWR NPYW  NR+ L  C+
Sbjct: 1   MASLFLTIISLLFAAFSSSVVEAAYSNGYTIPKLLPNPIDSCWRRNPYWASNRRALADCA 60

Query: 69  VGFVGKMTNNVGRNLIRYQVTDPSDDPINPRPGTLRYGATMMKSKVWITFQKDMHILLHK 128
           VGF GK     G+    Y VT+PSDDP NPRPGTLRY A +    +WITF +DM I+L  
Sbjct: 61  VGF-GKSAVG-GKYGSIYVVTNPSDDPENPRPGTLRY-AVIQSKPLWITFARDMVIVLRN 120

Query: 129 PLLISSYTAIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVS 188
            L+++SY  IDGRGA V I+   C+ +   +++IIHG+ IH CK   +G+V    + + S
Sbjct: 121 ELIMNSYKTIDGRGAKVEIAYGPCITIQHVSHVIIHGISIHDCKPGKSGRVRSSPTHVGS 180

Query: 189 LGHVDGDAIRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHE 248
               DGDAI +  +S +WIDH    RC+DGL+DV   ST +TISNN+F   DKV+LLGH 
Sbjct: 181 RKGSDGDAIAIFDSSHIWIDHCFFSRCQDGLIDVLHASTAVTISNNYFTQHDKVMLLGHN 240

Query: 249 DGYVRDMNMRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSIVWS 308
           D  V D  MRVT+ +NHFGP   +RMPR+R GYAHVANN Y+ W  YAIGGS +P+I +S
Sbjct: 241 DNNVEDKIMRVTIAFNHFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSADPTI-FS 300

Query: 309 TGKVEAA--------------------KWKFHSVRDVFENGASFGQIGVGRGGVKPNYSA 368
            G    A                    +WK+ + +DVF+NGA F  +  G G V P Y  
Sbjct: 301 EGNYFVASDDPSKKQVTKRIDSGYDWKRWKWRTSKDVFKNGAYF--VPSGYGTVTPLYGR 360

Query: 369 QQRFPVADAKWVRSLTSSSGALRCSSKSRC 373
            +RFPV+    V  LTSS+G L C S   C
Sbjct: 361 AERFPVSHGSLVPLLTSSAGPLHCYSGRIC 384

BLAST of Cp4.1LG20g00270 vs. TAIR 10
Match: AT1G30350.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 307.8 bits (787), Expect = 1.2e-83
Identity = 173/374 (46.26%), Postives = 222/374 (59.36%), Query Frame = 0

Query: 14  LFVLSLISLTLLSAVSYS-EGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNN 73
           L + +  S  L +A SY+     + +N ID CWR NP W  NRQ L  C+VG+ GK    
Sbjct: 10  LLLAAFASPLLETAHSYNVTAPRVSLNPIDACWRRNPKWATNRQALAHCAVGY-GKAAIG 69

Query: 74  VGRNLIRYQVTDPSDDPINPRPGTLRYGATMMKSKVWITFQKDMHILLHKPLLISSYTAI 133
            G+N   Y VT+PSD+P  P PGTLRY  +  K  +WITF +DM I+L   L+I+SY  I
Sbjct: 70  -GKNGPIYVVTNPSDNPTRPSPGTLRYAVSQPK-PLWITFARDMVIVLKSQLMINSYKTI 129

Query: 134 DGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDAIR 193
           DGRGA V I+   CL + +  ++IIHG+ IH CKA        PN        +DGD IR
Sbjct: 130 DGRGAKVEIANGPCLRIRQVKHVIIHGISIHDCKAD-------PNG-------MDGDGIR 189

Query: 194 LVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRDMNMR 253
           +  ++ VWIDH  L RC DGL+DV   ST +TISNN+F   DKV+LLGH+D Y+ D +MR
Sbjct: 190 VFQSTHVWIDHCFLSRCHDGLIDVIVSSTAVTISNNYFTQHDKVMLLGHDDSYMGDKDMR 249

Query: 254 VTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSIVWSTGKV------ 313
           VT+ +N FGP   +RMPR+R GYAHVANN Y+ W  YAIGGS NP I++S G        
Sbjct: 250 VTIAFNTFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSANP-IIFSEGNYFVAPEK 309

Query: 314 --------------EAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAK 367
                         ++ +WK+ + RDVF NGA FG  GV    V+P Y   + F VA   
Sbjct: 310 RSSKQVTKRMMAGPDSKRWKWGTSRDVFMNGAFFGPPGV---IVRPLYKGGEGFRVAHGS 362

BLAST of Cp4.1LG20g00270 vs. TAIR 10
Match: AT4G22080.1 (root hair specific 14 )

HSP 1 Score: 300.1 bits (767), Expect = 2.5e-81
Identity = 158/354 (44.63%), Postives = 203/354 (57.34%), Query Frame = 0

Query: 38  MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNVGRNLIRYQVTDPSDDPINPRPGTL 97
           MN +D CWR    W  NR+ L  C VGF          NL  Y VT+P D+  NP+PG+L
Sbjct: 46  MNPVDSCWRLKSDWAANRKDLADCVVGFGSSTLGGKKGNL--YVVTNPYDNAQNPQPGSL 105

Query: 98  RYGATMMKSKVWITFQKDMHILLHKPLLISSYTAIDGRGASVHISGNACLVVSKATNIII 157
           RYG    K  +WITF KDM I L   L+++SY  IDGRGA V I+   C+ +   TN+I+
Sbjct: 106 RYGVIQAK-PLWITFAKDMVITLENELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVIV 165

Query: 158 HGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDAIRLVSASKVWIDHNTLYRCEDGLLDVT 217
           HG+ IH CK    G V    + +      DGDAI +  +S +WIDH  L  C DGL+DV 
Sbjct: 166 HGISIHDCKPGKYGMVRSSPTHVGHRKGSDGDAIAIFGSSNIWIDHCYLASCTDGLIDVI 225

Query: 218 RGSTDITISNNWFRDQDKVILLGHEDGYVRDMNMRVTVVYNHFGPNCNQRMPRIRHGYAH 277
             ST ITISNN+F   DKV+LLGH D +V+D+ M+VTV +NHFGP   +RMPR+R GYAH
Sbjct: 226 HASTGITISNNYFTQHDKVMLLGHNDDFVQDVKMKVTVAFNHFGPGLVERMPRVRRGYAH 285

Query: 278 VANNLYQGWTQYAIGGSMNPSIVWSTGKVEAA-------------------KWKFHSVRD 337
           VANN Y  W  YAIGGS +P+I +S G    A                    W++ + +D
Sbjct: 286 VANNRYDKWIMYAIGGSADPTI-FSEGNYFIASDKSNSKEVTKREVKGGWNNWRWRTSKD 345

Query: 338 VFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKWVRSLTSSSGALRCSSKSRC 373
           VF+NGA F  +  G G +   YS+ QRF VA    V SLT+ +G L C+    C
Sbjct: 346 VFKNGAYF--VPSGYGSISLPYSSAQRFTVAPGNLVPSLTADAGPLNCNRNGPC 393

BLAST of Cp4.1LG20g00270 vs. TAIR 10
Match: AT4G22090.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 299.7 bits (766), Expect = 3.3e-81
Identity = 158/354 (44.63%), Postives = 204/354 (57.63%), Query Frame = 0

Query: 38  MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNVGRNLIRYQVTDPSDDPINPRPGTL 97
           MN +D CWR    W  NR+ L  C+VGF          N+  Y VT+P D+  NP PG+L
Sbjct: 46  MNPVDSCWRLKSDWDVNREDLADCAVGFGSSTLGGKKGNI--YVVTNPYDNAQNPHPGSL 105

Query: 98  RYGATMMKSKVWITFQKDMHILLHKPLLISSYTAIDGRGASVHISGNACLVVSKATNIII 157
           RYG    K  +WITF KDM I L   L+++SY  IDGRGA V I+   C+ +   TN+I+
Sbjct: 106 RYGVIQAK-PLWITFAKDMVITLANELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVIV 165

Query: 158 HGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDAIRLVSASKVWIDHNTLYRCEDGLLDVT 217
           HG+ IH CK   +G+V    + +      DGDAI +  +S VWIDH  L  C DGL+DV 
Sbjct: 166 HGISIHDCKPGKSGKVRSSPTHVGHRKGSDGDAITIFGSSNVWIDHCYLASCTDGLIDVI 225

Query: 218 RGSTDITISNNWFRDQDKVILLGHEDGYVRDMNMRVTVVYNHFGPNCNQRMPRIRHGYAH 277
             ST ITISNN+F   DKV+LLGH D +V+D+ M+VTV +NHFGP   +RMPR+R GYAH
Sbjct: 226 HASTAITISNNYFTQHDKVMLLGHNDNFVKDVKMKVTVAFNHFGPGLVERMPRVRRGYAH 285

Query: 278 VANNLYQGWTQYAIGGSMNPSIVWSTGKVEAA-------------------KWKFHSVRD 337
           VANN Y  W  YAIGGS +P+I +S G    A                    W++ +  D
Sbjct: 286 VANNRYDKWIMYAIGGSADPTI-FSEGNYFIASDKSYSKEVTKREVKGGWNNWRWRTSND 345

Query: 338 VFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKWVRSLTSSSGALRCSSKSRC 373
           VF+NGA F  +  G G +   YS+ QRF VA    V SLT+ +G L C+    C
Sbjct: 346 VFKNGAFF--VPSGYGSIPLPYSSAQRFTVAPGNLVPSLTADAGPLNCNRNGPC 393

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O653889.7e-8645.38Putative pectate lyase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g11920 PE=3 SV=2[more]
Q9C8G41.7e-8246.26Probable pectate lyase 4 OS=Arabidopsis thaliana OX=3702 GN=At1g30350 PE=2 SV=1[more]
O654563.6e-8044.63Probable pectate lyase 16 OS=Arabidopsis thaliana OX=3702 GN=At4g22080 PE=2 SV=1[more]
O654574.7e-8044.63Putative pectate lyase 17 OS=Arabidopsis thaliana OX=3702 GN=At4g22090 PE=3 SV=1[more]
Q9LRM52.3e-7944.78Probable pectate lyase 9 OS=Arabidopsis thaliana OX=3702 GN=At3g24230 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023520094.11.24e-26996.04putative pectate lyase 2 [Cucurbita pepo subsp. pepo][more]
KAG7020030.14.16e-26895.51putative pectate lyase 2, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022923821.15.90e-26895.25putative pectate lyase 2 [Cucurbita moschata][more]
XP_023001119.11.89e-26494.20putative pectate lyase 2 [Cucurbita maxima][more]
KAG6584442.18.27e-25699.14putative pectate lyase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1E7R92.86e-26895.25Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111431420 PE=3 SV=1[more]
A0A6J1KLU69.16e-26594.20Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111495358 PE=3 SV=1[more]
A0A1S3AY181.69e-23181.96Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103483978 PE=3 SV=1[more]
A0A0A0LPZ11.82e-23181.17Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_1G031910 PE=3 SV=1[more]
A0A6J1KAY16.29e-22281.82Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111492658 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G09280.11.3e-10975.32Pectin lyase-like superfamily protein [more]
AT1G11920.16.9e-8745.38Pectin lyase-like superfamily protein [more]
AT1G30350.11.2e-8346.26Pectin lyase-like superfamily protein [more]
AT4G22080.12.5e-8144.63root hair specific 14 [more]
AT4G22090.13.3e-8144.63Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 289..308
score: 46.19
coord: 123..139
score: 52.1
coord: 267..286
score: 62.62
coord: 187..208
score: 48.7
coord: 86..111
score: 41.94
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 115..312
e-value: 1.8E-59
score: 213.6
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 128..296
e-value: 3.0E-22
score: 79.2
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 38..365
e-value: 5.0E-86
score: 290.8
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 20..369
NoneNo IPR availablePANTHERPTHR31683:SF74PECTATE LYASEcoord: 20..369
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 38..363

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG20g00270.1Cp4.1LG20g00270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity