Cp4.1LG19g11060 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG19g11060
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionRING-type E3 ubiquitin transferase
LocationCp4.1LG19: 5233725 .. 5238198 (+)
RNA-Seq ExpressionCp4.1LG19g11060
SyntenyCp4.1LG19g11060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGGTCTCTACTCTACAATTAACGAATACGAGACTGTAATTTGTAAATTACAAGTTCAAGACTGGTTTCTTCGTGCTTTCATTTGCATTCCACTTGCTTGTGGATTCTTCATAAACTGCTTGGATACTAGACCTCCCTCTGACCAAGCTATTCCTTATTCTTCACTTGTTATATCAGACAACAGGTGAGGGAAGCAAAAAATCAAAGCTACCAAGTGGCAGTGGGCTGATAGCTAACACTAAGGATACTTGGAAGGTAAAATCCGAGTCATCGACTGGCGCAACTTTGATTGTACTGCTGAAGGGAACTAAAAATCTTACCTCAAATGTTGATCACAAAAGCTCAAAGTTAAATATCTGAAAGATTTGTTCCTGAAATGGTTTCTTCTCCTGTACTAAAGGTTAATGCTGATCACACATGCACCAAAGCCATAGTTGAAAAGTTGGTCGAAGACTTGTCGAGCCATACACTTGAAACTCTAAGGACTACTACAGCTGAACTGCGGTTGCTTACCAAGCTTAATGCGAAAAATCGGAGGGTGATTTCGGACTGTGGGGCAATTAGCCTTTTAGTCAATTTGCTAAACTCCACTGACGCACAAGTTCAAGAGAATGCTGTTACAGCACTTGTGAACTTGTCAATTGACAATAATTGCAAAATTATCATTGCTCAGGCCAATGCAATTGAAGCTTTGATTCACGTCCTTCAAACTGGTAGCCCAGAGGCAAAGGAAAACTCAGCTGCTACTCTTGGTAGTCTTTCAGTATGTGAGGATAACCAGGTTAAAATTGGGAGGTCTGGGGCAATAGGGCCTCTGGTTGACTTGTTGAGGGATGGCACACCTCGAGGAAAGAAAGATGCAGCCACTGCTCTGTTTAATTTGTCATTGCTTTCCGAAAACAAGGCTAAAATTGTGGAGGCTGGATCTATCAAGCATCTTGTGAAGTTGATGGATCCAGCTGCTGGAATGGTTGAGAAGGCAGTGGTTGTTCTGGCAAATGTTGCATCAATAGATGAAGGGAGGATTGAAATTGTACGCGAGGGTGGTATTCCTTTGTTGGTTGACGCTGTTGAGTTAGGTTCTGCCAGGGCAAAAGAGTATGCAGCTGCTGCATTATTGTGGCTTTGTGCAATCTCCAGTAGGTTCTGCATCATGGCAATCCGAGAGGGAGCTGTCCCACCATTAGTGGCTTTGTCACAATCAGGCACAACAAGGGCCAAGGAAAAGGTACATGACTATACTGAATTGTGTTTTCCATGATTCCAAATTCACAATAGATTTTAAAAAGGAAAATTTGGCCACTTGCAGGCTAGGGCACTACTTGGCTGCTTCAGTAGAAATAAGCTCGTATGAGAATGCTAAAAGTCTGGAAGTGACTAATTCTTAAACAAGGTTTGGCTGTTTCCATTGCGTTCATGGTGTACGATAGGATTGCTGTTAGGATAGAAATGGAAACATTAATGTGTATTAAGAGAATATTAGTATTTAGCTAAGGAGTTGTTAGTGGAGTTTGGTTATAAATAGAATGAGTCTTGAAGGGGCAATTGTTTAGTGTTTAGGCTTCAGTGATACCTTGAGACAACAGAGGTTTTAGTACTTCGAATTACTCAGGTTATCTTGCAGTTCTTCTATCTTTTATATTTCAGCATATTCGGGTTTTATCAATGTATTTACGTTCAGATGTTGCGTACATATGGGTTATTGCTCTGTAATTACTGGTGCAAAAAGAAAAGAAAAACGAGGGTATAATAAAGGAAGGATAGTTTTTATTGTTTCATGTTAAAAAATTGGTATTCCAATGTACAAGACGAAGGATTTGTTGTTGTTCTGAGCTTTAGGATTTCTCTGTCTCTCTCTCTTTTTTGTTGGTATTTGCATGTATTTTTTTCTTCCCCTTGTCCCATCAAAAAGGTTTTATGGGTAACTTATTTATCTAAAATTTCAAGCACCAAAATGAACACGAGCTGATGTGCTTGTGCATCTCGATCCTTGTCGAACAGATCTTTCAGTGTTGACGATTCATCTTCCTGATGAAGCATCAGACACAAAGTATTATTTTTTATTGCTTTTAGGGAGAAAAAATTCACCTAATTCTGGAAGCCCTTTTCTGTTTGATTCTATGCCACTATATATGGACTTATCAGAGAGCTTCCACTTGAATGGTCTTAGCTTTAATATGTTTCAGTCTGATTCAATGAATTTATTTGTTCATCTCACAGACACACTTACATTTAAGAAACTGCAGGGGAAAAGGAATTTTATTCAAGTGATTGAAAAGTGAAAACATGACAGATTTCAAGGGAGGAAAATTAAATGAAAAATAAAATCAAGGGCATTTAGATTTTCCCTTGGAGTTCAACTTGTCTCTGTAGCAAGGACATTCTGACTTGTTTCCATAGGTTCCAGAGGGCACACACTTACATTGTTGGCAGCAGAGTCCACAATACTTAAGACACCTGTCCTTCACCCCTGCATTCAAACATCTTGCCCCACATTTTGAATCACAAAAACCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAGGAAAAACAATAAATCAGTTATTCTCTTGGATGAATGAAGGGTTATATCTTCATTGCTAATAGTATTGATATTAATGGTAAAGCATTTGTCTTACTTGGAGCATAGGTTGCAGAGGAATCAGCAATGGCATGTTGGAGGAGGAAGGAGCTCAGAATCAATGAAACAAGAACCAAACTGAGAGAACCAGACTTCATCTTTCTCCTTTTGTTTTAAGGTTAGTGTTCTACTGACTTTTCATTACTTTCTATCTGATATTTATAGACTTAGTAAGTGGAGGACTGGAATGAGAGAGGGAGGAATAGGTCCGTAAAGAATTGAAAAGTGGGGCTTGAATCAGTTGGATGACCCATGAGGGAAAGGCATGGAAAAATGTTTTAAGAATGGTTTACAGCTCGAACTAAAGTGTAAAGAAGCATGGTTTAGGTANTTTTTTTTTTTTTTTTTGTATATGTTCCTTAGGAAGGTCACTAGCAGCTTCTTTTTGTGTCCTTGTGCAGTGTATTTTATGGGGGTAATGGGAATGTGTTTCTGATGTATTGATCTGAACGTGGATTAGTTAGGAGGCTTTCAATCTATCAAATGCCCATTCAACTTATTGAAAATAGAAATCTTTAGTGATCAAATTTGAAATGAAGTAAGCTTTAGGGATGAAAACTACATTTTTTCCTGTTCTACAAATCTGAATTTGAACCCTTTTTCTGTTTGGAAAAGAGCTTGAACTAATTCTATGTTATATTATGCTGTGAACTAAATTTTTTTAAGCTGAACAAACCCATAAGAACTAAGTGGTAATTCTTAGCAACTGAGAAATATATGGAGTAGTTGAAATTGTATATATAGGCTTGAGCTCTTTCTTCCCCCACTCTGGATTCCACAATTGAGAAATATAAAATGGGACGTTTTCAATTATTGTACTTTCTGTATTTCTTCATTTTAAATGAACCTTTGCGTGTGAGTACATGTAAGATTCTGTACTTCATGTTATGTTATCAATATAAAATTGAGCTGTTTTTGACGATCTATGAAGGATATAAATAATGCTAGATCACTTCAAACTTTGTGTACTTTTGTACTAATGCTATATTGTAAAGAGAAAACTACTTGATTTTGGTTCAAGAGTAAGTTTGAGATGCCACACTGACTAAGATATTAGCAAGTGAAGTTCAAATTTTCACTTATCATTAGAACTCAACTTTATTTCTTTGATTTTTCCTGAAACTCCTCCCTGTACCCTAATCTCTCTTCCTTCATCTATCTTAGGGGTATGAGATCGGTAAAGACCAGAACAGACTGAACCGACTTGGGTCGGTCGGTTTATGTCATTTTTTAGTCGAGTTAGTTGGGTCGACCCTAAATAGCAGCCTCCTTTAGGAAGTCCAAACCGATGATCTTCCCAAGGTGATGTAGTTATTTATTATTATTTTTATTATTGTTTTTGTCTATTTTCTATTCTGTTCTGCTTTTTTTTTTTCTAGGCTAGGGGAGAACATAATCGGACTGACAAGCTGAACTGAACCGATTAAAGTTCTTAAAGTTTTGGTTCAGTTTGGTTCAAATTTTAAAGTTTTTCAATCTGAATCGGTTCTGTTCTACTTCAACTGACTTAGTTAAAAGCTTAGAGTAGTTAAATCTATGAAAACGTAAACATGCCCAACATAGCAAGTATTTCTAAAGCATATTTAAAGGATAGAACTTTGATTACTTGAAAACTTGAAAATTGGAATAGTTCCTAGTAAATTTGATCAAGTTTGATAGGGTGATCCTAAAGATAATCGAAGGACTCTATTGTAGCATTAAATGACTAAAGAAGCTCACAACTTTATTCAGACGGTTATTCCAAATTTCAAAGCAAGGATTATATTGAAGTTGAAGGTTGGATTGGATTCTTTCCTTAGTATGGAAGTTGAAATGCTATCTTATTACATCA

mRNA sequence

ATGATGACAACAGGTGAGGGAAGCAAAAAATCAAAGCTACCAAGTGGCAGTGGGCTGATAGCTAACACTAAGGATACTTGGAAGGTTAATGCTGATCACACATGCACCAAAGCCATAGTTGAAAAGTTGGTCGAAGACTTGTCGAGCCATACACTTGAAACTCTAAGGACTACTACAGCTGAACTGCGGTTGCTTACCAAGCTTAATGCGAAAAATCGGAGGGTGATTTCGGACTGTGGGGCAATTAGCCTTTTAGTCAATTTGCTAAACTCCACTGACGCACAAGTTCAAGAGAATGCTGTTACAGCACTTGTGAACTTGTCAATTGACAATAATTGCAAAATTATCATTGCTCAGGCCAATGCAATTGAAGCTTTGATTCACGTCCTTCAAACTGGTAGCCCAGAGGCAAAGGAAAACTCAGCTGCTACTCTTGGTAGTCTTTCAGTATGTGAGGATAACCAGGTTAAAATTGGGAGGTCTGGGGCAATAGGGCCTCTGGTTGACTTGTTGAGGGATGGCACACCTCGAGGAAAGAAAGATGCAGCCACTGCTCTGTTTAATTTGTCATTGCTTTCCGAAAACAAGGCTAAAATTGTGGAGGCTGGATCTATCAAGCATCTTGTGAAGTTGATGGATCCAGCTGCTGGAATGGTTGAGAAGGCAGTGGTTGTTCTGGCAAATGTTGCATCAATAGATGAAGGGAGGATTGAAATTGTACGCGAGGGTGGTATTCCTTTGTTGGTTGACGCTGTTGAGTTAGGTTCTGCCAGGGCAAAAGAGTATGCAGCTGCTGCATTATTGTGGCTTTGTGCAATCTCCAGTAGGTTCTGCATCATGGCAATCCGAGAGGGAGCTGTCCCACCATTAGTGGCTTTGTCACAATCAGGCACAACAAGGGCCAAGGAAAAGGGGTATGAGATCGGTAAAGACCAGAACAGACTGAACCGACTTGGGTCGGTCGGTTTATGTCATTTTTTAGTCGAGTTAGTTGGGTCGACCCTAAATAGCAGCCTCCTTTAGGAAGTCCAAACCGATGATCTTCCCAAGGTGATGTAGTTATTTATTATTATTTTTATTATTGTTTTTGTCTATTTTCTATTCTGTTCTGCTTTTTTTTTTTCTAGGCTAGGGGAGAACATAATCGGACTGACAAGCTGAACTGAACCGATTAAAGTTCTTAAAGTTTTGGTTCAGTTTGGTTCAAATTTTAAAGTTTTTCAATCTGAATCGGTTCTGTTCTACTTCAACTGACTTAGTTAAAAGCTTAGAGTAGTTAAATCTATGAAAACGTAAACATGCCCAACATAGCAAGTATTTCTAAAGCATATTTAAAGGATAGAACTTTGATTACTTGAAAACTTGAAAATTGGAATAGTTCCTAGTAAATTTGATCAAGTTTGATAGGGTGATCCTAAAGATAATCGAAGGACTCTATTGTAGCATTAAATGACTAAAGAAGCTCACAACTTTATTCAGACGGTTATTCCAAATTTCAAAGCAAGGATTATATTGAAGTTGAAGGTTGGATTGGATTCTTTCCTTAGTATGGAAGTTGAAATGCTATCTTATTACATCA

Coding sequence (CDS)

ATGATGACAACAGGTGAGGGAAGCAAAAAATCAAAGCTACCAAGTGGCAGTGGGCTGATAGCTAACACTAAGGATACTTGGAAGGTTAATGCTGATCACACATGCACCAAAGCCATAGTTGAAAAGTTGGTCGAAGACTTGTCGAGCCATACACTTGAAACTCTAAGGACTACTACAGCTGAACTGCGGTTGCTTACCAAGCTTAATGCGAAAAATCGGAGGGTGATTTCGGACTGTGGGGCAATTAGCCTTTTAGTCAATTTGCTAAACTCCACTGACGCACAAGTTCAAGAGAATGCTGTTACAGCACTTGTGAACTTGTCAATTGACAATAATTGCAAAATTATCATTGCTCAGGCCAATGCAATTGAAGCTTTGATTCACGTCCTTCAAACTGGTAGCCCAGAGGCAAAGGAAAACTCAGCTGCTACTCTTGGTAGTCTTTCAGTATGTGAGGATAACCAGGTTAAAATTGGGAGGTCTGGGGCAATAGGGCCTCTGGTTGACTTGTTGAGGGATGGCACACCTCGAGGAAAGAAAGATGCAGCCACTGCTCTGTTTAATTTGTCATTGCTTTCCGAAAACAAGGCTAAAATTGTGGAGGCTGGATCTATCAAGCATCTTGTGAAGTTGATGGATCCAGCTGCTGGAATGGTTGAGAAGGCAGTGGTTGTTCTGGCAAATGTTGCATCAATAGATGAAGGGAGGATTGAAATTGTACGCGAGGGTGGTATTCCTTTGTTGGTTGACGCTGTTGAGTTAGGTTCTGCCAGGGCAAAAGAGTATGCAGCTGCTGCATTATTGTGGCTTTGTGCAATCTCCAGTAGGTTCTGCATCATGGCAATCCGAGAGGGAGCTGTCCCACCATTAGTGGCTTTGTCACAATCAGGCACAACAAGGGCCAAGGAAAAGGGGTATGAGATCGGTAAAGACCAGAACAGACTGAACCGACTTGGGTCGGTCGGTTTATGTCATTTTTTAGTCGAGTTAGTTGGGTCGACCCTAAATAGCAGCCTCCTTTAG

Protein sequence

MMTTGEGSKKSKLPSGSGLIANTKDTWKVNADHTCTKAIVEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVNLLNSTDAQVQENAVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGSLSVCEDNQVKIGRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKHLVKLMDPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAALLWLCAISSRFCIMAIREGAVPPLVALSQSGTTRAKEKGYEIGKDQNRLNRLGSVGLCHFLVELVGSTLNSSLL
Homology
BLAST of Cp4.1LG19g11060 vs. ExPASy Swiss-Prot
Match: O22193 (U-box domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUB4 PE=1 SV=3)

HSP 1 Score: 327.0 bits (837), Expect = 2.5e-88
Identity = 186/265 (70.19%), Postives = 217/265 (81.89%), Query Frame = 0

Query: 40  VEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVNLLNSTDAQVQEN 99
           V+KLVE+L S +L+T R  TAELRLL K N  NR VI + GAI LLV LL STD+  QEN
Sbjct: 543 VKKLVEELKSSSLDTQRQATAELRLLAKHNMDNRIVIGNSGAIVLLVELLYSTDSATQEN 602

Query: 100 AVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGSLSVCEDNQVKIG 159
           AVTAL+NLSI++N K  IA A AIE LIHVL+ GS EAKENSAATL SLSV E+N++KIG
Sbjct: 603 AVTALLNLSINDNNKKAIADAGAIEPLIHVLENGSSEAKENSAATLFSLSVIEENKIKIG 662

Query: 160 RSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKHLVKLMDPAAGMV 219
           +SGAIGPLVDLL +GTPRGKKDAATALFNLS+  ENKA IV++G++++L+ LMDPAAGMV
Sbjct: 663 QSGAIGPLVDLLGNGTPRGKKDAATALFNLSIHQENKAMIVQSGAVRYLIDLMDPAAGMV 722

Query: 220 EKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAALLWLCAISSRFCI 279
           +KAV VLAN+A+I EGR  I +EGGIPLLV+ VELGSAR KE AAAALL L   S RFC 
Sbjct: 723 DKAVAVLANLATIPEGRNAIGQEGGIPLLVEVVELGSARGKENAAAALLQLSTNSGRFCN 782

Query: 280 MAIREGAVPPLVALSQSGTTRAKEK 305
           M ++EGAVPPLVALSQSGT RA+EK
Sbjct: 783 MVLQEGAVPPLVALSQSGTPRAREK 807

BLAST of Cp4.1LG19g11060 vs. ExPASy Swiss-Prot
Match: Q5XEZ8 (U-box domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=PUB2 PE=2 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 4.9e-76
Identity = 163/272 (59.93%), Postives = 208/272 (76.47%), Query Frame = 0

Query: 40  VEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVNLLNSTDAQVQEN 99
           V+KL++DL S +L+T R  TA +R+L + +  NR VI+ C AI  LV+LL STD ++Q +
Sbjct: 423 VKKLIDDLKSSSLDTQREATARIRILARNSTDNRIVIARCEAIPSLVSLLYSTDERIQAD 482

Query: 100 AVTALVNLSIDNNCKIIIAQANAIEALIHVLQTG-SPEAKENSAATLGSLSVCEDNQVKI 159
           AVT L+NLSI++N K +IA++ AI  LIHVL+TG   EAK NSAATL SLSV E+ + +I
Sbjct: 483 AVTCLLNLSINDNNKSLIAESGAIVPLIHVLKTGYLEEAKANSAATLFSLSVIEEYKTEI 542

Query: 160 GRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKHLVKLMDPAAGM 219
           G +GAI PLVDLL  G+  GKKDAATALFNLS+  ENK K++EAG++++LV+LMDPA GM
Sbjct: 543 GEAGAIEPLVDLLGSGSLSGKKDAATALFNLSIHHENKTKVIEAGAVRYLVELMDPAFGM 602

Query: 220 VEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAALLWLCAISSRFC 279
           VEKAVVVLAN+A++ EG+I I  EGGIP+LV+ VELGSAR KE A AALL LC  S +FC
Sbjct: 603 VEKAVVVLANLATVREGKIAIGEEGGIPVLVEVVELGSARGKENATAALLQLCTHSPKFC 662

Query: 280 IMAIREGAVPPLVALSQSGTTRAKEKGYEIGK 311
              IREG +PPLVAL++SGT R KEK   + K
Sbjct: 663 NNVIREGVIPPLVALTKSGTARGKEKAQNLLK 694

BLAST of Cp4.1LG19g11060 vs. ExPASy Swiss-Prot
Match: Q8GWV5 (U-box domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=PUB3 PE=2 SV=2)

HSP 1 Score: 258.1 bits (658), Expect = 1.4e-67
Identity = 153/276 (55.43%), Postives = 201/276 (72.83%), Query Frame = 0

Query: 34  TCTKAIVEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVNLLNSTD 93
           T T +   KLVEDL S + +      AE+R LT  + +NR  I  CGAI+ L++LL S +
Sbjct: 468 TMTTSHTIKLVEDLKSGSNKVKTAAAAEIRHLTINSIENRVHIGRCGAITPLLSLLYSEE 527

Query: 94  AQVQENAVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGSLSVCED 153
              QE+AVTAL+NLSI    K +I +  AIE L+HVL TG+  AKENSAA+L SLSV + 
Sbjct: 528 KLTQEHAVTALLNLSISELNKAMIVEVGAIEPLVHVLNTGNDRAKENSAASLFSLSVLQV 587

Query: 154 NQVKIGRS-GAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKHLVKLM 213
           N+ +IG+S  AI  LV+LL  GT RGKKDAA+ALFNLS+  +NKA+IV+A ++K+LV+L+
Sbjct: 588 NRERIGQSNAAIQALVNLLGKGTFRGKKDAASALFNLSITHDNKARIVQAKAVKYLVELL 647

Query: 214 DPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAALLWLCA 273
           DP   MV+KAV +LAN++++ EGR  IVREGGIPLLV+ V+LGS R KE AA+ LL LC 
Sbjct: 648 DPDLEMVDKAVALLANLSAVGEGRQAIVREGGIPLLVETVDLGSQRGKENAASVLLQLCL 707

Query: 274 ISSRFCIMAIREGAVPPLVALSQSGTTRAKEKGYEI 309
            S +FC + ++EGA+PPLVALSQSGT RAKEK  ++
Sbjct: 708 NSPKFCTLVLQEGAIPPLVALSQSGTQRAKEKAQQL 743

BLAST of Cp4.1LG19g11060 vs. ExPASy Swiss-Prot
Match: Q5VRH9 (U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 4.0e-54
Identity = 128/267 (47.94%), Postives = 176/267 (65.92%), Query Frame = 0

Query: 43  LVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVNLLNSTDAQVQENAVT 102
           L+  L S   +  R    E+RLL K N  NR  I++ GAI LLVNLL+S+D + QE+AVT
Sbjct: 328 LMNRLRSGNQDEQRAAAGEIRLLAKRNVNNRICIAEAGAIPLLVNLLSSSDPRTQEHAVT 387

Query: 103 ALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGSLSVCEDNQVKIGRSG 162
           AL+NLSI  N K  I  ++AI  ++ VL+TGS E +EN+AATL SLSV ++N+V IG +G
Sbjct: 388 ALLNLSIHENNKASIVDSHAIPKIVEVLKTGSMETRENAAATLFSLSVVDENKVTIGAAG 447

Query: 163 AIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKHLVK-LMDPAAGMVEK 222
           AI PL++LL DG+PRGKKDAATA+FNL +   NK + V+AG + HL+  L+DP  GM+++
Sbjct: 448 AIPPLINLLCDGSPRGKKDAATAIFNLCIYQGNKVRAVKAGIVIHLMNFLVDPTGGMIDE 507

Query: 223 AVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAALLWLCAISSRFCIMA 282
           A+ +L+ +A   EG+I I R   IP LV+ ++ GS R +E AAA L  LC+  +   + A
Sbjct: 508 ALSLLSILAGNPEGKIVIARSEPIPPLVEVIKTGSPRNRENAAAILWLLCSADTEQTLAA 567

Query: 283 IREGAVPPLVALSQSGTTRAKEKGYEI 309
              G    L  LS++GT RAK K   I
Sbjct: 568 KAAGVEDALKELSETGTDRAKRKASSI 594

BLAST of Cp4.1LG19g11060 vs. ExPASy Swiss-Prot
Match: Q9SNC6 (U-box domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=PUB13 PE=1 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 1.1e-51
Identity = 127/277 (45.85%), Postives = 181/277 (65.34%), Query Frame = 0

Query: 40  VEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVNLLNSTDAQVQEN 99
           +E L+  L+    E  R+   E+RLL K NA NR  I++ GAI LLV LL++ D+++QE+
Sbjct: 354 IEDLMWRLAYGNPEDQRSAAGEIRLLAKRNADNRVAIAEAGAIPLLVGLLSTPDSRIQEH 413

Query: 100 AVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGSLSVCEDNQVKIG 159
           +VTAL+NLSI  N K  I  A AI  ++ VL+ GS EA+EN+AATL SLSV ++N+V IG
Sbjct: 414 SVTALLNLSICENNKGAIVSAGAIPGIVQVLKKGSMEARENAAATLFSLSVIDENKVTIG 473

Query: 160 RSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKHLVKLM-DPAAGM 219
             GAI PLV LL +GT RGKKDAATALFNL +   NK K + AG I  L +L+ +P +GM
Sbjct: 474 ALGAIPPLVVLLNEGTQRGKKDAATALFNLCIYQGNKGKAIRAGVIPTLTRLLTEPGSGM 533

Query: 220 VEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAALLWLCAISSRFC 279
           V++A+ +LA ++S  EG+  I     +P LV+ +  GS R +E AAA L+ LC+   +  
Sbjct: 534 VDEALAILAILSSHPEGKAIIGSSDAVPSLVEFIRTGSPRNRENAAAVLVHLCSGDPQHL 593

Query: 280 IMAIREGAVPPLVALSQSGTTRAKEKGYEIGKDQNRL 316
           + A + G + PL+ L+ +GT R K K  ++ +  +RL
Sbjct: 594 VEAQKLGLMGPLIDLAGNGTDRGKRKAAQLLERISRL 630

BLAST of Cp4.1LG19g11060 vs. NCBI nr
Match: XP_023518122.1 (U-box domain-containing protein 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 560 bits (1442), Expect = 2.72e-198
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 0

Query: 3   TTGEGSKKSKLPSGSGLIANTKDTWKVNADHTCTKAIVEKLVEDLSSHTLETLRTTTAEL 62
           TTGEGSKKSKLPSGSGLIANTKDTWKVNADHTCTKAIVEKLVEDLSSHTLETLRTTTAEL
Sbjct: 73  TTGEGSKKSKLPSGSGLIANTKDTWKVNADHTCTKAIVEKLVEDLSSHTLETLRTTTAEL 132

Query: 63  RLLTKLNAKNRRVISDCGAISLLVNLLNSTDAQVQENAVTALVNLSIDNNCKIIIAQANA 122
           RLLTKLNAKNRRVISDCGAISLLVNLLNSTDAQVQENAVTALVNLSIDNNCKIIIAQANA
Sbjct: 133 RLLTKLNAKNRRVISDCGAISLLVNLLNSTDAQVQENAVTALVNLSIDNNCKIIIAQANA 192

Query: 123 IEALIHVLQTGSPEAKENSAATLGSLSVCEDNQVKIGRSGAIGPLVDLLRDGTPRGKKDA 182
           IEALIHVLQTGSPEAKENSAATLGSLSVCEDNQVKIGRSGAIGPLVDLLRDGTPRGKKDA
Sbjct: 193 IEALIHVLQTGSPEAKENSAATLGSLSVCEDNQVKIGRSGAIGPLVDLLRDGTPRGKKDA 252

Query: 183 ATALFNLSLLSENKAKIVEAGSIKHLVKLMDPAAGMVEKAVVVLANVASIDEGRIEIVRE 242
           ATALFNLSLLSENKAKIVEAGSIKHLVKLMDPAAGMVEKAVVVLANVASIDEGRIEIVRE
Sbjct: 253 ATALFNLSLLSENKAKIVEAGSIKHLVKLMDPAAGMVEKAVVVLANVASIDEGRIEIVRE 312

Query: 243 GGIPLLVDAVELGSARAKEYAAAALLWLCAISSRFCIMAIREGAVPPLVALSQSGTTRAK 302
           GGIPLLVDAVELGSARAKEYAAAALLWLCAISSRFCIMAIREGAVPPLVALSQSGTTRAK
Sbjct: 313 GGIPLLVDAVELGSARAKEYAAAALLWLCAISSRFCIMAIREGAVPPLVALSQSGTTRAK 372

Query: 303 EK 304
           EK
Sbjct: 373 EK 374

BLAST of Cp4.1LG19g11060 vs. NCBI nr
Match: KAG6595003.1 (U-box domain-containing protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 506 bits (1303), Expect = 1.50e-178
Identity = 275/277 (99.28%), Postives = 275/277 (99.28%), Query Frame = 0

Query: 28  KVNADHTCTKAIVEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVN 87
           KVNADHTCTKAIVEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVN
Sbjct: 8   KVNADHTCTKAIVEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVN 67

Query: 88  LLNSTDAQVQENAVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGS 147
           LLNSTDAQVQENAVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGS
Sbjct: 68  LLNSTDAQVQENAVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGS 127

Query: 148 LSVCEDNQVKIGRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKH 207
           LSVCEDNQV IGRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKH
Sbjct: 128 LSVCEDNQVNIGRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKH 187

Query: 208 LVKLMDPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAAL 267
           LVKLMDPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAAL
Sbjct: 188 LVKLMDPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAAL 247

Query: 268 LWLCAISSRFCIMAIREGAVPPLVALSQSGTTRAKEK 304
           LWLCAISSRFCIMAIREGAVPPLVALSQSGT RAKEK
Sbjct: 248 LWLCAISSRFCIMAIREGAVPPLVALSQSGTARAKEK 284

BLAST of Cp4.1LG19g11060 vs. NCBI nr
Match: XP_022963300.1 (U-box domain-containing protein 4-like [Cucurbita moschata] >XP_022963301.1 U-box domain-containing protein 4-like [Cucurbita moschata] >XP_022963302.1 U-box domain-containing protein 4-like [Cucurbita moschata] >XP_022963304.1 U-box domain-containing protein 4-like [Cucurbita moschata] >XP_022963305.1 U-box domain-containing protein 4-like [Cucurbita moschata])

HSP 1 Score: 499 bits (1285), Expect = 8.23e-176
Identity = 271/277 (97.83%), Postives = 272/277 (98.19%), Query Frame = 0

Query: 28  KVNADHTCTKAIVEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVN 87
           KVNADHTCTKAIVEKLVEDL SHTLE LRTTTAELRLLTKLNAKNRRVISDCGAISLLVN
Sbjct: 8   KVNADHTCTKAIVEKLVEDLLSHTLEILRTTTAELRLLTKLNAKNRRVISDCGAISLLVN 67

Query: 88  LLNSTDAQVQENAVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGS 147
           LLNSTD QVQENAVTALVNLSIDNNCKII+AQANAIEALIHVLQTGSPEAKENSAATLGS
Sbjct: 68  LLNSTDTQVQENAVTALVNLSIDNNCKIILAQANAIEALIHVLQTGSPEAKENSAATLGS 127

Query: 148 LSVCEDNQVKIGRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKH 207
           LSVCEDNQV IGRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKH
Sbjct: 128 LSVCEDNQVNIGRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKH 187

Query: 208 LVKLMDPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAAL 267
           LVKLMDPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAAL
Sbjct: 188 LVKLMDPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAAL 247

Query: 268 LWLCAISSRFCIMAIREGAVPPLVALSQSGTTRAKEK 304
           LWLCAISSRFCIMAIREGAVPPLVALSQSGT RAKEK
Sbjct: 248 LWLCAISSRFCIMAIREGAVPPLVALSQSGTARAKEK 284

BLAST of Cp4.1LG19g11060 vs. NCBI nr
Match: XP_023003128.1 (U-box domain-containing protein 4-like [Cucurbita maxima] >XP_023003129.1 U-box domain-containing protein 4-like [Cucurbita maxima] >XP_023003131.1 U-box domain-containing protein 4-like [Cucurbita maxima] >XP_023003132.1 U-box domain-containing protein 4-like [Cucurbita maxima])

HSP 1 Score: 499 bits (1285), Expect = 8.23e-176
Identity = 272/277 (98.19%), Postives = 273/277 (98.56%), Query Frame = 0

Query: 28  KVNADHTCTKAIVEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVN 87
           KVNAD TCTKAIVEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAI+LLVN
Sbjct: 8   KVNADLTCTKAIVEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAINLLVN 67

Query: 88  LLNSTDAQVQENAVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGS 147
           LLNSTD QVQENAVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGS
Sbjct: 68  LLNSTDTQVQENAVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGS 127

Query: 148 LSVCEDNQVKIGRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKH 207
           LSVCEDNQV IGRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKH
Sbjct: 128 LSVCEDNQVNIGRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKH 187

Query: 208 LVKLMDPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAAL 267
           LVKLMDPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAAL
Sbjct: 188 LVKLMDPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAAL 247

Query: 268 LWLCAISSRFCIMAIREGAVPPLVALSQSGTTRAKEK 304
           LWLCAISSRFCIMAIREGAVPPLVALSQSGT RAKEK
Sbjct: 248 LWLCAISSRFCIMAIREGAVPPLVALSQSGTARAKEK 284

BLAST of Cp4.1LG19g11060 vs. NCBI nr
Match: KAG7026959.1 (U-box domain-containing protein 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 506 bits (1303), Expect = 2.48e-171
Identity = 275/276 (99.64%), Postives = 275/276 (99.64%), Query Frame = 0

Query: 29  VNADHTCTKAIVEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVNL 88
           VNADHTCTKAIVEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVNL
Sbjct: 508 VNADHTCTKAIVEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVNL 567

Query: 89  LNSTDAQVQENAVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGSL 148
           LNSTDAQVQENAVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGSL
Sbjct: 568 LNSTDAQVQENAVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGSL 627

Query: 149 SVCEDNQVKIGRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKHL 208
           SVCEDNQVKIGRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKHL
Sbjct: 628 SVCEDNQVKIGRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKHL 687

Query: 209 VKLMDPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAALL 268
           VKLMDPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAALL
Sbjct: 688 VKLMDPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAALL 747

Query: 269 WLCAISSRFCIMAIREGAVPPLVALSQSGTTRAKEK 304
           WLCAISSRFCIMAIREGAVPPLVALSQSGT RAKEK
Sbjct: 748 WLCAISSRFCIMAIREGAVPPLVALSQSGTARAKEK 783

BLAST of Cp4.1LG19g11060 vs. ExPASy TrEMBL
Match: A0A6J1KVK8 (U-box domain-containing protein 4-like OS=Cucurbita maxima OX=3661 GN=LOC111496834 PE=4 SV=1)

HSP 1 Score: 499 bits (1285), Expect = 3.99e-176
Identity = 272/277 (98.19%), Postives = 273/277 (98.56%), Query Frame = 0

Query: 28  KVNADHTCTKAIVEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVN 87
           KVNAD TCTKAIVEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAI+LLVN
Sbjct: 8   KVNADLTCTKAIVEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAINLLVN 67

Query: 88  LLNSTDAQVQENAVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGS 147
           LLNSTD QVQENAVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGS
Sbjct: 68  LLNSTDTQVQENAVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGS 127

Query: 148 LSVCEDNQVKIGRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKH 207
           LSVCEDNQV IGRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKH
Sbjct: 128 LSVCEDNQVNIGRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKH 187

Query: 208 LVKLMDPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAAL 267
           LVKLMDPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAAL
Sbjct: 188 LVKLMDPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAAL 247

Query: 268 LWLCAISSRFCIMAIREGAVPPLVALSQSGTTRAKEK 304
           LWLCAISSRFCIMAIREGAVPPLVALSQSGT RAKEK
Sbjct: 248 LWLCAISSRFCIMAIREGAVPPLVALSQSGTARAKEK 284

BLAST of Cp4.1LG19g11060 vs. ExPASy TrEMBL
Match: A0A6J1HHL5 (U-box domain-containing protein 4-like OS=Cucurbita moschata OX=3662 GN=LOC111463554 PE=4 SV=1)

HSP 1 Score: 499 bits (1285), Expect = 3.99e-176
Identity = 271/277 (97.83%), Postives = 272/277 (98.19%), Query Frame = 0

Query: 28  KVNADHTCTKAIVEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVN 87
           KVNADHTCTKAIVEKLVEDL SHTLE LRTTTAELRLLTKLNAKNRRVISDCGAISLLVN
Sbjct: 8   KVNADHTCTKAIVEKLVEDLLSHTLEILRTTTAELRLLTKLNAKNRRVISDCGAISLLVN 67

Query: 88  LLNSTDAQVQENAVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGS 147
           LLNSTD QVQENAVTALVNLSIDNNCKII+AQANAIEALIHVLQTGSPEAKENSAATLGS
Sbjct: 68  LLNSTDTQVQENAVTALVNLSIDNNCKIILAQANAIEALIHVLQTGSPEAKENSAATLGS 127

Query: 148 LSVCEDNQVKIGRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKH 207
           LSVCEDNQV IGRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKH
Sbjct: 128 LSVCEDNQVNIGRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKH 187

Query: 208 LVKLMDPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAAL 267
           LVKLMDPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAAL
Sbjct: 188 LVKLMDPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAAL 247

Query: 268 LWLCAISSRFCIMAIREGAVPPLVALSQSGTTRAKEK 304
           LWLCAISSRFCIMAIREGAVPPLVALSQSGT RAKEK
Sbjct: 248 LWLCAISSRFCIMAIREGAVPPLVALSQSGTARAKEK 284

BLAST of Cp4.1LG19g11060 vs. ExPASy TrEMBL
Match: A0A0A0KFV9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G493830 PE=4 SV=1)

HSP 1 Score: 427 bits (1098), Expect = 2.57e-147
Identity = 231/274 (84.31%), Postives = 252/274 (91.97%), Query Frame = 0

Query: 31  ADHTCTKAIVEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVNLLN 90
           +DHT  KA V+KLVE+L+S + ETLRT+TAELRLLTK++A NR +I+D GAISLLVNLLN
Sbjct: 31  SDHTGIKAKVQKLVENLTSDSPETLRTSTAELRLLTKIDANNRTLIADYGAISLLVNLLN 90

Query: 91  STDAQVQENAVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGSLSV 150
           STD ++QENAVTALVNLSIDNNCK II QANAIE LIHVLQTGSPEAKENSAATLGSLSV
Sbjct: 91  STDTKIQENAVTALVNLSIDNNCKSIIVQANAIEPLIHVLQTGSPEAKENSAATLGSLSV 150

Query: 151 CEDNQVKIGRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKHLVK 210
            +DNQV IGRS AIGPLVDLL+DGTPRGK+DAATALFNLSLLSENK KIVEAGSIKHLVK
Sbjct: 151 VDDNQVNIGRSRAIGPLVDLLKDGTPRGKRDAATALFNLSLLSENKPKIVEAGSIKHLVK 210

Query: 211 LMDPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAALLWL 270
           LMDPA GMVEKAV VLAN+AS DEGRIEIVREGGIPLLVD +ELGSARAKEYAAAALLWL
Sbjct: 211 LMDPATGMVEKAVTVLANLASTDEGRIEIVREGGIPLLVDTIELGSARAKEYAAAALLWL 270

Query: 271 CAISSRFCIMAIREGAVPPLVALSQSGTTRAKEK 304
           C I+SR+CIMAI+EGA+PPLVALSQSGT RAKEK
Sbjct: 271 CGITSRYCIMAIQEGAIPPLVALSQSGTARAKEK 304

BLAST of Cp4.1LG19g11060 vs. ExPASy TrEMBL
Match: A0A5D3CR20 (U-box domain-containing protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004380 PE=4 SV=1)

HSP 1 Score: 426 bits (1096), Expect = 5.00e-147
Identity = 233/280 (83.21%), Postives = 254/280 (90.71%), Query Frame = 0

Query: 28  KVNADHTCTKAIVEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVN 87
           +V +D T  KA V+KLVEDL S +LETLRT+TAELRLLTK++A N  VI+D GAISLLVN
Sbjct: 28  EVISDLTGIKAKVQKLVEDLRSDSLETLRTSTAELRLLTKIDANNWTVIADYGAISLLVN 87

Query: 88  LLNSTDAQVQENAVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGS 147
           LLNSTD ++QENAVTALVNLSIDNNCK II QANAIE LIHVLQTGSPEAKENSAATLGS
Sbjct: 88  LLNSTDTKIQENAVTALVNLSIDNNCKTIIVQANAIEPLIHVLQTGSPEAKENSAATLGS 147

Query: 148 LSVCEDNQVKIGRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKH 207
           LSV +DNQV IGRSGAIGPLVDLL+DGTPRGK+DAATALFNLSLLSENK KIVEAGSIKH
Sbjct: 148 LSVVDDNQVNIGRSGAIGPLVDLLKDGTPRGKRDAATALFNLSLLSENKPKIVEAGSIKH 207

Query: 208 LVKLMDPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAAL 267
           LVKLMDPA GMVEKAV VLAN+AS DEGRIEIVREGGI LLVD +ELGSARAKEYAAAAL
Sbjct: 208 LVKLMDPATGMVEKAVTVLANLASTDEGRIEIVREGGIALLVDTIELGSARAKEYAAAAL 267

Query: 268 LWLCAISSRFCIMAIREGAVPPLVALSQSGTTRAKEKGYE 307
           LWLC I+SR+CIMAI+EGA+PPLVALSQSGT RAKEK ++
Sbjct: 268 LWLCGITSRYCIMAIQEGAIPPLVALSQSGTARAKEKVHD 307

BLAST of Cp4.1LG19g11060 vs. ExPASy TrEMBL
Match: A0A1S3B0V3 (U-box domain-containing protein 4 OS=Cucumis melo OX=3656 GN=LOC103484819 PE=4 SV=1)

HSP 1 Score: 426 bits (1095), Expect = 7.10e-147
Identity = 233/277 (84.12%), Postives = 252/277 (90.97%), Query Frame = 0

Query: 28  KVNADHTCTKAIVEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVN 87
           +V +D T  KA V+KLVEDL S +LETLRT+TAELRLLTK++A N  VI+D GAISLLVN
Sbjct: 28  EVISDLTGIKAKVQKLVEDLRSDSLETLRTSTAELRLLTKIDANNWTVIADYGAISLLVN 87

Query: 88  LLNSTDAQVQENAVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGS 147
           LLNSTD ++QENAVTALVNLSIDNNCK II QANAIE LIHVLQTGSPEAKENSAATLGS
Sbjct: 88  LLNSTDTKIQENAVTALVNLSIDNNCKTIIVQANAIEPLIHVLQTGSPEAKENSAATLGS 147

Query: 148 LSVCEDNQVKIGRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKH 207
           LSV +DNQV IGRSGAIGPLVDLL+DGTPRGK+DAATALFNLSLLSENK KIVEAGSIKH
Sbjct: 148 LSVVDDNQVNIGRSGAIGPLVDLLKDGTPRGKRDAATALFNLSLLSENKPKIVEAGSIKH 207

Query: 208 LVKLMDPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAAL 267
           LVKLMDPA GMVEKAV VLAN+AS DEGRIEIVREGGI LLVD +ELGSARAKEYAAAAL
Sbjct: 208 LVKLMDPATGMVEKAVTVLANLASTDEGRIEIVREGGIALLVDTIELGSARAKEYAAAAL 267

Query: 268 LWLCAISSRFCIMAIREGAVPPLVALSQSGTTRAKEK 304
           LWLC I+SR+CIMAI+EGA+PPLVALSQSGT RAKEK
Sbjct: 268 LWLCGITSRYCIMAIQEGAIPPLVALSQSGTARAKEK 304

BLAST of Cp4.1LG19g11060 vs. TAIR 10
Match: AT2G23140.1 (RING/U-box superfamily protein with ARM repeat domain )

HSP 1 Score: 327.0 bits (837), Expect = 1.8e-89
Identity = 186/265 (70.19%), Postives = 217/265 (81.89%), Query Frame = 0

Query: 40  VEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVNLLNSTDAQVQEN 99
           V+KLVE+L S +L+T R  TAELRLL K N  NR VI + GAI LLV LL STD+  QEN
Sbjct: 546 VKKLVEELKSSSLDTQRQATAELRLLAKHNMDNRIVIGNSGAIVLLVELLYSTDSATQEN 605

Query: 100 AVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGSLSVCEDNQVKIG 159
           AVTAL+NLSI++N K  IA A AIE LIHVL+ GS EAKENSAATL SLSV E+N++KIG
Sbjct: 606 AVTALLNLSINDNNKKAIADAGAIEPLIHVLENGSSEAKENSAATLFSLSVIEENKIKIG 665

Query: 160 RSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKHLVKLMDPAAGMV 219
           +SGAIGPLVDLL +GTPRGKKDAATALFNLS+  ENKA IV++G++++L+ LMDPAAGMV
Sbjct: 666 QSGAIGPLVDLLGNGTPRGKKDAATALFNLSIHQENKAMIVQSGAVRYLIDLMDPAAGMV 725

Query: 220 EKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAALLWLCAISSRFCI 279
           +KAV VLAN+A+I EGR  I +EGGIPLLV+ VELGSAR KE AAAALL L   S RFC 
Sbjct: 726 DKAVAVLANLATIPEGRNAIGQEGGIPLLVEVVELGSARGKENAAAALLQLSTNSGRFCN 785

Query: 280 MAIREGAVPPLVALSQSGTTRAKEK 305
           M ++EGAVPPLVALSQSGT RA+EK
Sbjct: 786 MVLQEGAVPPLVALSQSGTPRAREK 810

BLAST of Cp4.1LG19g11060 vs. TAIR 10
Match: AT2G23140.2 (RING/U-box superfamily protein with ARM repeat domain )

HSP 1 Score: 327.0 bits (837), Expect = 1.8e-89
Identity = 186/265 (70.19%), Postives = 217/265 (81.89%), Query Frame = 0

Query: 40  VEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVNLLNSTDAQVQEN 99
           V+KLVE+L S +L+T R  TAELRLL K N  NR VI + GAI LLV LL STD+  QEN
Sbjct: 543 VKKLVEELKSSSLDTQRQATAELRLLAKHNMDNRIVIGNSGAIVLLVELLYSTDSATQEN 602

Query: 100 AVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGSLSVCEDNQVKIG 159
           AVTAL+NLSI++N K  IA A AIE LIHVL+ GS EAKENSAATL SLSV E+N++KIG
Sbjct: 603 AVTALLNLSINDNNKKAIADAGAIEPLIHVLENGSSEAKENSAATLFSLSVIEENKIKIG 662

Query: 160 RSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKHLVKLMDPAAGMV 219
           +SGAIGPLVDLL +GTPRGKKDAATALFNLS+  ENKA IV++G++++L+ LMDPAAGMV
Sbjct: 663 QSGAIGPLVDLLGNGTPRGKKDAATALFNLSIHQENKAMIVQSGAVRYLIDLMDPAAGMV 722

Query: 220 EKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAALLWLCAISSRFCI 279
           +KAV VLAN+A+I EGR  I +EGGIPLLV+ VELGSAR KE AAAALL L   S RFC 
Sbjct: 723 DKAVAVLANLATIPEGRNAIGQEGGIPLLVEVVELGSARGKENAAAALLQLSTNSGRFCN 782

Query: 280 MAIREGAVPPLVALSQSGTTRAKEK 305
           M ++EGAVPPLVALSQSGT RA+EK
Sbjct: 783 MVLQEGAVPPLVALSQSGTPRAREK 807

BLAST of Cp4.1LG19g11060 vs. TAIR 10
Match: AT5G67340.1 (ARM repeat superfamily protein )

HSP 1 Score: 286.2 bits (731), Expect = 3.5e-77
Identity = 163/272 (59.93%), Postives = 208/272 (76.47%), Query Frame = 0

Query: 40  VEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVNLLNSTDAQVQEN 99
           V+KL++DL S +L+T R  TA +R+L + +  NR VI+ C AI  LV+LL STD ++Q +
Sbjct: 423 VKKLIDDLKSSSLDTQREATARIRILARNSTDNRIVIARCEAIPSLVSLLYSTDERIQAD 482

Query: 100 AVTALVNLSIDNNCKIIIAQANAIEALIHVLQTG-SPEAKENSAATLGSLSVCEDNQVKI 159
           AVT L+NLSI++N K +IA++ AI  LIHVL+TG   EAK NSAATL SLSV E+ + +I
Sbjct: 483 AVTCLLNLSINDNNKSLIAESGAIVPLIHVLKTGYLEEAKANSAATLFSLSVIEEYKTEI 542

Query: 160 GRSGAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKHLVKLMDPAAGM 219
           G +GAI PLVDLL  G+  GKKDAATALFNLS+  ENK K++EAG++++LV+LMDPA GM
Sbjct: 543 GEAGAIEPLVDLLGSGSLSGKKDAATALFNLSIHHENKTKVIEAGAVRYLVELMDPAFGM 602

Query: 220 VEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAALLWLCAISSRFC 279
           VEKAVVVLAN+A++ EG+I I  EGGIP+LV+ VELGSAR KE A AALL LC  S +FC
Sbjct: 603 VEKAVVVLANLATVREGKIAIGEEGGIPVLVEVVELGSARGKENATAALLQLCTHSPKFC 662

Query: 280 IMAIREGAVPPLVALSQSGTTRAKEKGYEIGK 311
              IREG +PPLVAL++SGT R KEK   + K
Sbjct: 663 NNVIREGVIPPLVALTKSGTARGKEKAQNLLK 694

BLAST of Cp4.1LG19g11060 vs. TAIR 10
Match: AT3G54790.1 (ARM repeat superfamily protein )

HSP 1 Score: 258.1 bits (658), Expect = 1.0e-68
Identity = 153/276 (55.43%), Postives = 201/276 (72.83%), Query Frame = 0

Query: 34  TCTKAIVEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVNLLNSTD 93
           T T +   KLVEDL S + +      AE+R LT  + +NR  I  CGAI+ L++LL S +
Sbjct: 468 TMTTSHTIKLVEDLKSGSNKVKTAAAAEIRHLTINSIENRVHIGRCGAITPLLSLLYSEE 527

Query: 94  AQVQENAVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGSLSVCED 153
              QE+AVTAL+NLSI    K +I +  AIE L+HVL TG+  AKENSAA+L SLSV + 
Sbjct: 528 KLTQEHAVTALLNLSISELNKAMIVEVGAIEPLVHVLNTGNDRAKENSAASLFSLSVLQV 587

Query: 154 NQVKIGRS-GAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKHLVKLM 213
           N+ +IG+S  AI  LV+LL  GT RGKKDAA+ALFNLS+  +NKA+IV+A ++K+LV+L+
Sbjct: 588 NRERIGQSNAAIQALVNLLGKGTFRGKKDAASALFNLSITHDNKARIVQAKAVKYLVELL 647

Query: 214 DPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAALLWLCA 273
           DP   MV+KAV +LAN++++ EGR  IVREGGIPLLV+ V+LGS R KE AA+ LL LC 
Sbjct: 648 DPDLEMVDKAVALLANLSAVGEGRQAIVREGGIPLLVETVDLGSQRGKENAASVLLQLCL 707

Query: 274 ISSRFCIMAIREGAVPPLVALSQSGTTRAKEKGYEI 309
            S +FC + ++EGA+PPLVALSQSGT RAKEK  ++
Sbjct: 708 NSPKFCTLVLQEGAIPPLVALSQSGTQRAKEKAQQL 743

BLAST of Cp4.1LG19g11060 vs. TAIR 10
Match: AT3G54790.2 (ARM repeat superfamily protein )

HSP 1 Score: 258.1 bits (658), Expect = 1.0e-68
Identity = 153/276 (55.43%), Postives = 201/276 (72.83%), Query Frame = 0

Query: 34  TCTKAIVEKLVEDLSSHTLETLRTTTAELRLLTKLNAKNRRVISDCGAISLLVNLLNSTD 93
           T T +   KLVEDL S + +      AE+R LT  + +NR  I  CGAI+ L++LL S +
Sbjct: 432 TMTTSHTIKLVEDLKSGSNKVKTAAAAEIRHLTINSIENRVHIGRCGAITPLLSLLYSEE 491

Query: 94  AQVQENAVTALVNLSIDNNCKIIIAQANAIEALIHVLQTGSPEAKENSAATLGSLSVCED 153
              QE+AVTAL+NLSI    K +I +  AIE L+HVL TG+  AKENSAA+L SLSV + 
Sbjct: 492 KLTQEHAVTALLNLSISELNKAMIVEVGAIEPLVHVLNTGNDRAKENSAASLFSLSVLQV 551

Query: 154 NQVKIGRS-GAIGPLVDLLRDGTPRGKKDAATALFNLSLLSENKAKIVEAGSIKHLVKLM 213
           N+ +IG+S  AI  LV+LL  GT RGKKDAA+ALFNLS+  +NKA+IV+A ++K+LV+L+
Sbjct: 552 NRERIGQSNAAIQALVNLLGKGTFRGKKDAASALFNLSITHDNKARIVQAKAVKYLVELL 611

Query: 214 DPAAGMVEKAVVVLANVASIDEGRIEIVREGGIPLLVDAVELGSARAKEYAAAALLWLCA 273
           DP   MV+KAV +LAN++++ EGR  IVREGGIPLLV+ V+LGS R KE AA+ LL LC 
Sbjct: 612 DPDLEMVDKAVALLANLSAVGEGRQAIVREGGIPLLVETVDLGSQRGKENAASVLLQLCL 671

Query: 274 ISSRFCIMAIREGAVPPLVALSQSGTTRAKEKGYEI 309
            S +FC + ++EGA+PPLVALSQSGT RAKEK  ++
Sbjct: 672 NSPKFCTLVLQEGAIPPLVALSQSGTQRAKEKAQQL 707

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O221932.5e-8870.19U-box domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUB4 PE=1 S... [more]
Q5XEZ84.9e-7659.93U-box domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=PUB2 PE=2 S... [more]
Q8GWV51.4e-6755.43U-box domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=PUB3 PE=2 S... [more]
Q5VRH94.0e-5447.94U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=P... [more]
Q9SNC61.1e-5145.85U-box domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=PUB13 PE=1... [more]
Match NameE-valueIdentityDescription
XP_023518122.12.72e-198100.00U-box domain-containing protein 4-like [Cucurbita pepo subsp. pepo][more]
KAG6595003.11.50e-17899.28U-box domain-containing protein 4, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022963300.18.23e-17697.83U-box domain-containing protein 4-like [Cucurbita moschata] >XP_022963301.1 U-bo... [more]
XP_023003128.18.23e-17698.19U-box domain-containing protein 4-like [Cucurbita maxima] >XP_023003129.1 U-box ... [more]
KAG7026959.12.48e-17199.64U-box domain-containing protein 4, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
A0A6J1KVK83.99e-17698.19U-box domain-containing protein 4-like OS=Cucurbita maxima OX=3661 GN=LOC1114968... [more]
A0A6J1HHL53.99e-17697.83U-box domain-containing protein 4-like OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
A0A0A0KFV92.57e-14784.31Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G493830 PE=4 SV=1[more]
A0A5D3CR205.00e-14783.21U-box domain-containing protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3B0V37.10e-14784.12U-box domain-containing protein 4 OS=Cucumis melo OX=3656 GN=LOC103484819 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT2G23140.11.8e-8970.19RING/U-box superfamily protein with ARM repeat domain [more]
AT2G23140.21.8e-8970.19RING/U-box superfamily protein with ARM repeat domain [more]
AT5G67340.13.5e-7759.93ARM repeat superfamily protein [more]
AT3G54790.11.0e-6855.43ARM repeat superfamily protein [more]
AT3G54790.21.0e-6855.43ARM repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000225ArmadilloSMARTSM00185arm_5coord: 192..231
e-value: 0.0058
score: 25.9
coord: 151..191
e-value: 0.3
score: 20.1
coord: 69..109
e-value: 6.3E-5
score: 32.4
coord: 232..272
e-value: 5.7
score: 15.1
coord: 110..150
e-value: 0.024
score: 23.8
IPR000225ArmadilloPFAMPF00514Armcoord: 110..149
e-value: 3.1E-6
score: 27.0
coord: 153..190
e-value: 8.8E-6
score: 25.6
coord: 69..108
e-value: 7.1E-11
score: 41.8
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 121..163
score: 9.0124
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 80..122
score: 12.0924
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 203..244
score: 10.2724
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 162..204
score: 10.2024
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 112..215
e-value: 2.2E-26
score: 94.8
coord: 216..310
e-value: 8.4E-14
score: 53.5
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 21..111
e-value: 1.3E-11
score: 46.2
NoneNo IPR availablePANTHERPTHR23315:SF280RING-TYPE E3 UBIQUITIN TRANSFERASEcoord: 25..307
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 25..307
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 36..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG19g11060.1Cp4.1LG19g11060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity