Cp4.1LG19g08170 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG19g08170
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionPyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta
LocationCp4.1LG19: 6741673 .. 6747058 (+)
RNA-Seq ExpressionCp4.1LG19g08170
SyntenyCp4.1LG19g08170
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAATGTCCAATGTCCGGGAGAGAGCTGTCAACGCATCAAAGGAAAGAGTCCAAACTGAATTATTACTTTTTCCATTTTCCAACACAGTTTCGGTTCAGCATGGTAATGTCCAGCTCCGACAATTCGCTATATTTCAGGCGTTTAACCACCGGTGTTGAGCATCGTAGTCTCAGCCTCCCGCACCATCACTTTCTCTGGTGTTCGTGCTTGCTCTAGTTATACAATGCCAAGTTCTTTCGTCTCCAATGGTGAAGCCGCCGTCAAATCTTCTCCTGCGGCGGTGTCCGGACGGGTCTCCTCAGTGTATGCTGAAGTTCAGTCCAGTCGTATCGACCAAAATCTGCCTCTGCCTTCTGTTTTCAGAAGTTCCTTCACGATCGTCGATGGACCCCCTAGCTCTGCCGCTGGCAATCCAGGTACTCTGTTCTATTGCATGCTCTCGGGATCTGTGTATGTATCACTTTTGTTTCATCTTATAGTGTTTATTATTACTTTAGATCCTTGAAGAATCTGGTTGCTGCTTTGCTGAATCAGGAATGAGTTTTTAGCAATTTTAAGTGTCTTTTCGATTTTATTCGATCGAATGGGAACACTAAAAATGGAAGCAGTTGCTTACGCTTCAGAGTTTTCTGTTCAGTCAATACATATTTTGGTGTCTGTTTCTGATTTCATAGACTTACGTGAATTTTTGCGATGCATGAGTCTTGTATCTCCTTCCAATATTCCTGTGCATGGATTTGGAAGGAGGACTACAATTTAGGCGTTTTATTAATCTTCTTTGCGTTTGTTTTAAGCAGACGAGATAGCCAAGCTATTTCCTAATCTTTTCGGCCAGCCATCGGCAAAATTGGTACCGAGCAAATCCAACAAAGCTCAATCGGACAAGAAGTTGAAGATTGGTGTCGTGTTGTCTGGAGGCCAGGCCCCCGGAGGACATAACGTGATTTCCGGAATTTTTGGTAACGATTATTTTACATTTACTGAATTAAATATGATAATTCTATGCTAATCTACTTTTGAATCGTGTTTCTTTTATTATTTGGATTTGAGTGATTTTAAATCTGTGTTGGCGTTAGATTACTTGCAGGATCATGTCAAAGGCAGTGTGTTATATGGCTTCAAGGGTGGTCCTGCCGGGATTATGAAATGTAATTATGTGGAATTGACTGCAGAATATATTTATCCATATAGAAATCAGGTAAACCTATAGAGTGCTTCACAACTTTAAACATTTAAATACTAGTAACGATGTTGACCTCTAATGGATTTCGATACTCTATATTAATACTTTGTTATCATACATCACTATGTAAAATAAGTTAACTGGTGCATGAGCATAATTGATGCTGGTTATTGAGATCTTTTCAGGGTGGATTTGATATGATACGCAGTGGGAGGGACAAATTTGAAACAACAGAGCAGGTGAGTCTGTTTCTTTTCAACGTTCAGACATTATGCTGAAGTGACTTGGATATATGAACTTAGTTGAGTGGGCTTAATTTTTCAGTTCAAACAAGCTGAAGAAACTGCAAAAAAGCTTGATTTGGATGGGATTGTTGTGATTGGTGGAGATGATTCCAATACAAATGCCTGCCTTCTTGCTGAAAACTTCAGGTGGACTGCTCTATTTAAGAAAATTTGCTTTTCCGTACATTGTTTTAGTTGTATCTATCATATGTTTTGTGAAATAATACCTCTTCTTTTGATCAGGAGTAAAAATTTGAAGACCCGGGTTATTGGTTGCCCCAAAACAATTGATGGAGATCTAAAATGCAAAGAGGTGCCCATAAGTTTCGGATTTGATACAGCTTGCAGGGTAACATTTCTCTCGTTTTATTCTTAACATGGTGTTAATTCTTTTGATGTAAACTACACATCATTGCCCTATGATTGTTCGAATGTTTTTTTCAATTTTGTTCTTGTTGTGGTAGTAGATATTACCATCTCGAGTTTTTGCTCAAGTGTTAACACATCTCCTTGAACTTGTGATTTCTTACGAAGTAGAAGGATTTAGACTTGATATTACTTTGCAGAGAGAATAAAGTTGTCTGGGTTTTACAGTTTTACTAGATTGGATTTGCACTACGTTGTTCATTTGCCACTATTAACGAAGTTCATGTTTCTAATGTGGCAGATTTATGCAGAAATGATTGGTAATGTCATGATAGATGCTAGATCAACTGGAAAATATTATCATTGTAAGAAAGTCCTATATCTTTACCTGACGGATTAAAATGTTATTTTTTTTTTAATCTATTCATAATGCTTATTTACGTTGTGGATACATTTATTTATCAGCCAATAAACTGTTTGATGCCTCAAGAAAGAGGCATTTATAAGTTGTTATGTTAGTACATAGCTCTAAAGAAGCGCTTTAGCATTCATGTGGATCATTGATTTGTTTTTACTTTGTCGTATTTCTCTTAGCATATTATTTGATTTGTGAATCTGCAGTTGTGAGGCTTATGGGACGTGCTGCTTCACACATCACCCTAGAGTGTGCTCTGCAGACTCATCCAAATATTACTATTATTGGGGAAGAGGTACTATTAGCTTCTTGTCAGCTGCATTTTTAATTATGTTTTTTTTTTTCTTTTTTTTTTCTGTGGTGTCCTCACATTCTACTCTCGCTCATTGCAAGTGATTCTAAATGCTCGATTTGTGTCATCTTTTAATGTATGGAGCCTTCTTAAAACTTTATGATTGATTGGGAATCTCTGGTCGATGGTTATAAATTTTGCTGCATGTAGAGGCTACAGTGTTCCTAACTACTAGGATTGATTTGCTGCAGGTTTCTGCTCAAAAGCAAACGCTGAAAAGTGTTACAGATTACATTGTAGATGTAGTCTGCAAACGTGCAGAACTGGGTTTTAATTATGGTGTTATACTTATTCCGGAAGGCCTTATTGACTTCATCCCGGAGGTATTATTCTTGTTCGTAAAATCGGTAATGGCTGTCTTATTATCTTCATTCTTAGCTGTGTGTTTAATGAAACTATATGTTGCTCTTGAATGCCTTTCAGGTACAGCAACTTATTGCAGAGCTAAATGAAATTCTGGCCCATGAAATAGTTGACGATGAAGGATTATGGAAAAAGAAACTCACTAGTCAATCTCTTGAACTTTTTGAGCTCTTGCCTCAAGCAATCCAAGAACAATTGATGCTCGAGAGAGATCCACATGGAAATGTTCAGGTAATTTTCTTTTTCCTTCTGTTTTTGTTTTGATACCCAGGTGCTGGTGTTTCTTGGTTAGATATGATATTTTTCTTCTTTTCTTGTTTAGGTAGCTAAAATAGAAACAGAGAAAATGCTCATTCAGATGGTTGAAACAGAGTTGGAGAAGAGGAAGAGTGAAGGTGCATACAATGGTCAATTTAAAGGGCAATCTCACTTTTTTGGGTAAGTATTCTTCTCATTATTTGAGAACTGCAGTTCACTTTCTTTTTCTCTTTCTTTTCCTTTTGTTGGAGTTCCACAGCAACACTTGTGCGTCAAACTTGCAGATATGAAGGGAGGTGTGGTCTACCAACCAATTTCGATTCCACTTACTGCTATGCATTGGGTTATGGTGCTGGAGCTCTCCTGCAGAGTGGAAAAACTGGACTAATATCATCCGTCTGTCCTATTCTCTGCTCATTTTATCTTCAATATCTCTGCAAACACTTCCATCAAATATCAGTTTCCTGTTATTCCTAATTTTTTTGGCTGTGTTTGTCAATAATTTGTCGGTAAATATTTGTGCATACTATGTGTATCTGCCAGGTGGGGAACTTAGCTGCTCCCGTTGAAGAATGGACTGTTGGTGGGACTTCATTGACATCATTAATGGATGTGGAGAGGAGACATGGTAGACTACAGCTTGCTATCTTAATCACTCTGTTATCTCTATCATGCATGGTCTTAATTTTAGTCGTCTTGTGGTTTCTTTCAACCCCCTTCGTTTGCATGCTCCCTCAAAGTGGTTTGTCCATTTTCATAAAGAATGTGTGAAAATTCTAAGGTTCAGAATTAGCTCTTTCTTTAGATCTCAAGGATAAAGTTGTACAACCCTTGGAACAGGTCCTGTTCAAGGTCGTAGAAATGGCCTTTTCTTGCAAGTCATCTTTAAGAAAGGTGCTACCAAATGTATACCCGTACATTACAGGTCAAACATAAACAAAATACTTTGGGTCGGTGTGTCTGCTTGGCATAAATTTAAACGAAAGTCCAGCATCTATTGTTCTGGAGCTTTCAACTTCTAATAACATTAATGATACAAAAGGCCCTATTTCAATAATTGGTCTCTTTTTGGTTCATGATAACTTCATCATTATGGGACGCGGCTTAGCCCGAAGGTTCTTGAATCCTAGTCCAAATGTATTTAGCTGTCGCGTTCAAATGATTTGATTTTCATATACTTGTGTTATCACTGAAGTTGTATTTTTGTGACAGGCAAATTTAAGCCTGTGATTAAGAAGGCAATGGTCGAGCTCGATGGTAAGAACTGTGAAGGGAAGCTTTTTTTTTTTTTTTTCTTTTTTTCTTTTTTGTGTAATACTAGAATAGTGTCTTCCTGTTGTCATACTGACCAATCACTTTCATGTACTTATCTGCTACTGGAAAAAACTAAGCAATAATGTCCGATTCATTCATGAATTATCAGGTACCCCTTTCAAGAAGTTTGCATCATTGAGGGATGAGTGGGCATTCAACAACCGATACATTAGTCCTGGTTAGAAAACATTCAACTTCTATCTTTCTCTGATTCCTTGTCCGCTCTTAACAAAAAGAGAACAATCTCCTTGTCATGTTCTTCAGCTGGCTGCCATTTAATTTGTTTTCCCCTTTGCATTTACTAATATTTAAATGAGGGTGTTAAAAAAAACATGTCAATAATGCCTTAAAGATGAGAAAAGCATGCACTGTTTTGATTAAATTGTGACATTCTTCCTAATTGAAATTTCAGGTCCTATTCAATTTGTGGGTCCTGCATCAAGTGCTATTAATCATACTCTCCTCCTGGAGCTTGGAGCTGGAGCTTAAAGATAACTCCTTACCCAGTAGTTTCGACCCAAAATTTATTTCTTGAGGAAGCTTAAAGCCTGTTATGGCCTTTTTATGCGATATTTTAGGAACCTAGTTTTCTTCAATTTCAGCTTGATTAGCATTGCTCGGTGGCGTAAGATGCTAATAAAAAATCGCCACTGTTTTGGGGTATGATTTGAGTTCTGTAAGTTTAATGGGTTCTAACCATTAAATTTTTTTGAAGGATAGGGTTGCTGTCCAAGGACCAAATGTTAAAATTTCCAAGATTATCTGACTATGACTGCATACCAGTTCAAATAATGGAAATCGCCTAACATATTTGAATAAATTGATTCGACTTCTTACTTTATAATAAAACA

mRNA sequence

CAATGTCCAATGTCCGGGAGAGAGCTGTCAACGCATCAAAGGAAAGAGTCCAAACTGAATTATTACTTTTTCCATTTTCCAACACAGTTTCGGTTCAGCATGGTAATGTCCAGCTCCGACAATTCGCTATATTTCAGGCGTTTAACCACCGGTGTTGAGCATCGTAGTCTCAGCCTCCCGCACCATCACTTTCTCTGGTGTTCGTGCTTGCTCTAGTTATACAATGCCAAGTTCTTTCGTCTCCAATGGTGAAGCCGCCGTCAAATCTTCTCCTGCGGCGGTGTCCGGACGGGTCTCCTCAGTGTATGCTGAAGTTCAGTCCAGTCGTATCGACCAAAATCTGCCTCTGCCTTCTGTTTTCAGAAGTTCCTTCACGATCGTCGATGGACCCCCTAGCTCTGCCGCTGGCAATCCAGACGAGATAGCCAAGCTATTTCCTAATCTTTTCGGCCAGCCATCGGCAAAATTGGTACCGAGCAAATCCAACAAAGCTCAATCGGACAAGAAGTTGAAGATTGGTGTCGTGTTGTCTGGAGGCCAGGCCCCCGGAGGACATAACGTGATTTCCGGAATTTTTGATTACTTGCAGGATCATGTCAAAGGCAGTGTGTTATATGGCTTCAAGGGTGGTCCTGCCGGGATTATGAAATGTAATTATGTGGAATTGACTGCAGAATATATTTATCCATATAGAAATCAGGGTGGATTTGATATGATACGCAGTGGGAGGGACAAATTTGAAACAACAGAGCAGTTCAAACAAGCTGAAGAAACTGCAAAAAAGCTTGATTTGGATGGGATTGTTGTGATTGGTGGAGATGATTCCAATACAAATGCCTGCCTTCTTGCTGAAAACTTCAGGAGTAAAAATTTGAAGACCCGGGTTATTGGTTGCCCCAAAACAATTGATGGAGATCTAAAATGCAAAGAGGTGCCCATAAGTTTCGGATTTGATACAGCTTGCAGGATTTATGCAGAAATGATTGGTAATGTCATGATAGATGCTAGATCAACTGGAAAATATTATCATTTTGTGAGGCTTATGGGACGTGCTGCTTCACACATCACCCTAGAGTGTGCTCTGCAGACTCATCCAAATATTACTATTATTGGGGAAGAGGTACAGCAACTTATTGCAGAGCTAAATGAAATTCTGGCCCATGAAATAGTTGACGATGAAGGATTATGGAAAAAGAAACTCACTAGTCAATCTCTTGAACTTTTTGAGCTCTTGCCTCAAGCAATCCAAGAACAATTGATGCTCGAGAGAGATCCACATGGAAATGTTCAGGTAGCTAAAATAGAAACAGAGAAAATGCTCATTCAGATGGTTGAAACAGAGTTGGAGAAGAGGAAGAGTGAAGGTGCATACAATGGTCAATTTAAAGGGCAATCTCACTTTTTTGGATATGAAGGGAGGTGTGGTCTACCAACCAATTTCGATTCCACTTACTGCTATGCATTGGGTTATGGTGCTGGAGCTCTCCTGCAGAGTGGAAAAACTGGACTAATATCATCCGTGGGGAACTTAGCTGCTCCCGTTGAAGAATGGACTGTTGGTGGGACTTCATTGACATCATTAATGGATGTGGAGAGGAGACATGGCAAATTTAAGCCTGTGATTAAGAAGGCAATGGTCGAGCTCGATGGTACCCCTTTCAAGAAGTTTGCATCATTGAGGGATGAGTGGGCATTCAACAACCGATACATTAGTCCTGGTCCTATTCAATTTGTGGGTCCTGCATCAAGTGCTATTAATCATACTCTCCTCCTGGAGCTTGGAGCTGGAGCTTAAAGATAACTCCTTACCCAGTAGTTTCGACCCAAAATTTATTTCTTGAGGAAGCTTAAAGCCTGTTATGGCCTTTTTATGCGATATTTTAGGAACCTAGTTTTCTTCAATTTCAGCTTGATTAGCATTGCTCGGTGGCGTAAGATGCTAATAAAAAATCGCCACTGTTTTGGGGTATGATTTGAGTTCTGTAAGTTTAATGGGTTCTAACCATTAAATTTTTTTGAAGGATAGGGTTGCTGTCCAAGGACCAAATGTTAAAATTTCCAAGATTATCTGACTATGACTGCATACCAGTTCAAATAATGGAAATCGCCTAACATATTTGAATAAATTGATTCGACTTCTTACTTTATAATAAAACA

Coding sequence (CDS)

ATGCCAAGTTCTTTCGTCTCCAATGGTGAAGCCGCCGTCAAATCTTCTCCTGCGGCGGTGTCCGGACGGGTCTCCTCAGTGTATGCTGAAGTTCAGTCCAGTCGTATCGACCAAAATCTGCCTCTGCCTTCTGTTTTCAGAAGTTCCTTCACGATCGTCGATGGACCCCCTAGCTCTGCCGCTGGCAATCCAGACGAGATAGCCAAGCTATTTCCTAATCTTTTCGGCCAGCCATCGGCAAAATTGGTACCGAGCAAATCCAACAAAGCTCAATCGGACAAGAAGTTGAAGATTGGTGTCGTGTTGTCTGGAGGCCAGGCCCCCGGAGGACATAACGTGATTTCCGGAATTTTTGATTACTTGCAGGATCATGTCAAAGGCAGTGTGTTATATGGCTTCAAGGGTGGTCCTGCCGGGATTATGAAATGTAATTATGTGGAATTGACTGCAGAATATATTTATCCATATAGAAATCAGGGTGGATTTGATATGATACGCAGTGGGAGGGACAAATTTGAAACAACAGAGCAGTTCAAACAAGCTGAAGAAACTGCAAAAAAGCTTGATTTGGATGGGATTGTTGTGATTGGTGGAGATGATTCCAATACAAATGCCTGCCTTCTTGCTGAAAACTTCAGGAGTAAAAATTTGAAGACCCGGGTTATTGGTTGCCCCAAAACAATTGATGGAGATCTAAAATGCAAAGAGGTGCCCATAAGTTTCGGATTTGATACAGCTTGCAGGATTTATGCAGAAATGATTGGTAATGTCATGATAGATGCTAGATCAACTGGAAAATATTATCATTTTGTGAGGCTTATGGGACGTGCTGCTTCACACATCACCCTAGAGTGTGCTCTGCAGACTCATCCAAATATTACTATTATTGGGGAAGAGGTACAGCAACTTATTGCAGAGCTAAATGAAATTCTGGCCCATGAAATAGTTGACGATGAAGGATTATGGAAAAAGAAACTCACTAGTCAATCTCTTGAACTTTTTGAGCTCTTGCCTCAAGCAATCCAAGAACAATTGATGCTCGAGAGAGATCCACATGGAAATGTTCAGGTAGCTAAAATAGAAACAGAGAAAATGCTCATTCAGATGGTTGAAACAGAGTTGGAGAAGAGGAAGAGTGAAGGTGCATACAATGGTCAATTTAAAGGGCAATCTCACTTTTTTGGATATGAAGGGAGGTGTGGTCTACCAACCAATTTCGATTCCACTTACTGCTATGCATTGGGTTATGGTGCTGGAGCTCTCCTGCAGAGTGGAAAAACTGGACTAATATCATCCGTGGGGAACTTAGCTGCTCCCGTTGAAGAATGGACTGTTGGTGGGACTTCATTGACATCATTAATGGATGTGGAGAGGAGACATGGCAAATTTAAGCCTGTGATTAAGAAGGCAATGGTCGAGCTCGATGGTACCCCTTTCAAGAAGTTTGCATCATTGAGGGATGAGTGGGCATTCAACAACCGATACATTAGTCCTGGTCCTATTCAATTTGTGGGTCCTGCATCAAGTGCTATTAATCATACTCTCCTCCTGGAGCTTGGAGCTGGAGCTTAA

Protein sequence

MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSAAGNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDYLQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMIRSGRDKFETTEQFKQAEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPISFGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVQQLIAELNEILAHEIVDDEGLWKKKLTSQSLELFELLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNLAAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYISPGPIQFVGPASSAINHTLLLELGAGA
Homology
BLAST of Cp4.1LG19g08170 vs. ExPASy Swiss-Prot
Match: Q41141 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Ricinus communis OX=3988 GN=PFP-BETA PE=3 SV=1)

HSP 1 Score: 859.4 bits (2219), Expect = 2.2e-248
Identity = 431/547 (78.79%), Postives = 461/547 (84.28%), Query Frame = 0

Query: 21  SGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSAAGNPDEIAKLFPNLFGQPSA 80
           SGR +SVY+EVQSSRI+  LPLPSV    F IV GPPSSAAGNPDEIAKLFPNLFGQPSA
Sbjct: 6   SGRAASVYSEVQSSRIEHVLPLPSVLNHPFKIVQGPPSSAAGNPDEIAKLFPNLFGQPSA 65

Query: 81  KLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDYLQDHVKGSVLYGFKGGPAGI 140
            LVP  ++   S+++LKIG+VLSGGQAPGGHNVISGIFDYLQD  KGS+LYGF+GGPAGI
Sbjct: 66  MLVPDVADSLDSNQQLKIGLVLSGGQAPGGHNVISGIFDYLQDRAKGSILYGFRGGPAGI 125

Query: 141 MKCNYVELTAEYIYPYRNQGGFDMIRSGRDKFETTEQFKQAEETAKKLDLDGIVVIGGDD 200
           MKCNYV+LTA+YI+PYRNQGGFDMI SGRDK ET EQFKQAEETA KLDL+G+VVIGGDD
Sbjct: 126 MKCNYVQLTADYIHPYRNQGGFDMICSGRDKIETPEQFKQAEETAGKLDLNGLVVIGGDD 185

Query: 201 SNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPISFGFDTACRIYAEMIGNVMID 260
           SNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVP SFGFDTAC+IY+EMIGNVMID
Sbjct: 186 SNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPTSFGFDTACKIYSEMIGNVMID 245

Query: 261 ARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGE---------------------- 320
           ARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGE                      
Sbjct: 246 ARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVAAKKLALKDVTDYIVDVICK 305

Query: 321 ----------------------EVQQLIAELNEILAHEIVDDEGLWKKKLTSQSLELFEL 380
                                 EVQ LIAELNEILAH++VD+ GLWKKKLTSQSL+LFE 
Sbjct: 306 RADLGYNYGVILIPEGLIDFIPEVQNLIAELNEILAHDVVDEGGLWKKKLTSQSLQLFEF 365

Query: 381 LPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKSEGAYNGQFKGQSHFFGY 440
           LP AIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRK +G Y   FKGQSHFFGY
Sbjct: 366 LPVAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKQQGTYKAHFKGQSHFFGY 425

Query: 441 EGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNLAAPVEEWTVGGTSLTSLMDV 500
           EGRCGLPTNFDSTYCYALGY AGALL SGKTGLISSVGNL APV EWTVGGT+LTSLMDV
Sbjct: 426 EGRCGLPTNFDSTYCYALGYAAGALLHSGKTGLISSVGNLGAPVAEWTVGGTALTSLMDV 485

Query: 501 ERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYISPGPIQFVGPASSAINHTLL 524
           ERRHGKFKPVIKKAMVEL+G PFKKFASLR+EWA  NRY+SPGPIQF+GP S A +HTLL
Sbjct: 486 ERRHGKFKPVIKKAMVELEGAPFKKFASLREEWALKNRYVSPGPIQFMGPGSDAASHTLL 545

BLAST of Cp4.1LG19g08170 vs. ExPASy Swiss-Prot
Match: P21343 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Solanum tuberosum OX=4113 GN=PFP-BETA PE=1 SV=3)

HSP 1 Score: 847.8 bits (2189), Expect = 6.5e-245
Identity = 427/564 (75.71%), Postives = 469/564 (83.16%), Query Frame = 0

Query: 4   SFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSAAGN 63
           S ++NGE A  S  +  +GR ++VY+EVQ+SR+D  LPLPSV  S F +VDGPPSSAAG+
Sbjct: 7   SLLNNGELA-SSVKSPGTGRYAAVYSEVQNSRLDHPLPLPSVLGSPFKVVDGPPSSAAGH 66

Query: 64  PDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDYLQD 123
           P+EIAKLFP+L+GQP   LVP  S     ++ LKIGVVLSGGQAPGGHNVISGIFDYLQ 
Sbjct: 67  PEEIAKLFPSLYGQPCVSLVPDDSGDVAMNQILKIGVVLSGGQAPGGHNVISGIFDYLQT 126

Query: 124 HVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMIRSGRDKFETTEQFKQAEE 183
           H KGS +YGF+GGPAG+MK  YV LT E+IYPYRNQGGFDMI SGRDK ET EQFKQAEE
Sbjct: 127 HCKGSTMYGFRGGPAGVMKGKYVVLTPEFIYPYRNQGGFDMICSGRDKIETPEQFKQAEE 186

Query: 184 TAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPISFGF 243
           TAKKLDLDG+VVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLK KEVP SFGF
Sbjct: 187 TAKKLDLDGLVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKSKEVPTSFGF 246

Query: 244 DTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGE----- 303
           DTAC+IYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPN+T+IGE     
Sbjct: 247 DTACKIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNVTLIGEEVFAK 306

Query: 304 ---------------------------------------EVQQLIAELNEILAHEIVDDE 363
                                                  EVQQLIAELNEILAH++VD+ 
Sbjct: 307 KLTLKNVTDYIADVVCKRAESGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDVVDEA 366

Query: 364 GLWKKKLTSQSLELFELLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKS 423
           G+WKKKLT Q LELFELLP AIQEQL+LERDPHGNVQVAKIETEKMLIQMVETEL++RK 
Sbjct: 367 GVWKKKLTPQCLELFELLPLAIQEQLLLERDPHGNVQVAKIETEKMLIQMVETELDQRKQ 426

Query: 424 EGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNLAAP 483
           +GAYN QFKGQSHFFGYEGRCGLP+NFDSTYCYALGYGAG+LLQSGKTGLISSVGNLAAP
Sbjct: 427 KGAYNAQFKGQSHFFGYEGRCGLPSNFDSTYCYALGYGAGSLLQSGKTGLISSVGNLAAP 486

Query: 484 VEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYISPG 524
           VEEWTVGGT+LT+LMDVERRHGKFKPVIKKAMVEL+G PFKKFAS R+EWA NNRYI+PG
Sbjct: 487 VEEWTVGGTALTALMDVERRHGKFKPVIKKAMVELEGAPFKKFASKREEWALNNRYINPG 546

BLAST of Cp4.1LG19g08170 vs. ExPASy Swiss-Prot
Match: Q8W4M5 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 OS=Arabidopsis thaliana OX=3702 GN=PFP-BETA1 PE=1 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 1.4e-244
Identity = 431/564 (76.42%), Postives = 465/564 (82.45%), Query Frame = 0

Query: 2   PSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSAA 61
           P+  V+    AV S   A +   +SVY+EVQSSRI+  LPLPSV + +F IV+GP SSAA
Sbjct: 3   PALAVTRDLTAVGSPENAPAKGRASVYSEVQSSRINNTLPLPSVLKGAFKIVEGPASSAA 62

Query: 62  GNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDYL 121
           GNPDEIAKLFP L+GQPS  +VP + +   S  KLKIGVVLSGGQAPGGHNVISG+FDYL
Sbjct: 63  GNPDEIAKLFPGLYGQPSVAVVPDQ-DAPSSAPKLKIGVVLSGGQAPGGHNVISGLFDYL 122

Query: 122 QDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMIRSGRDKFETTEQFKQA 181
           Q+  KGS  YGFKGGPAGIMKC YVEL AEYI PYRNQGGFDMI SGRDK ET +QFKQA
Sbjct: 123 QERAKGSTFYGFKGGPAGIMKCKYVELNAEYIQPYRNQGGFDMICSGRDKIETPDQFKQA 182

Query: 182 EETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPISF 241
           EETAKKLDLDG+VVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVP SF
Sbjct: 183 EETAKKLDLDGLVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPTSF 242

Query: 242 GFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGE--- 301
           GFDTAC+IY+EMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGE   
Sbjct: 243 GFDTACKIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVS 302

Query: 302 -----------------------------------------EVQQLIAELNEILAHEIVD 361
                                                    EVQ+LIAELNEILA+E+VD
Sbjct: 303 AQKQTLKNVTDYMVDVICKRAELGYNYGVILIPEGLIDFIPEVQELIAELNEILANEVVD 362

Query: 362 DEGLWKKKLTSQSLELFELLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKR 421
           + GLWKKKLT QSL+LF+LLP+AIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKR
Sbjct: 363 ENGLWKKKLTEQSLKLFDLLPEAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKR 422

Query: 422 KSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNLA 481
           K  GAY GQF GQSHFFGYEGRCGLPTNFD+TYCYALGYGAG LL SGKTGLISSVGNLA
Sbjct: 423 KQAGAYKGQFMGQSHFFGYEGRCGLPTNFDATYCYALGYGAGVLLNSGKTGLISSVGNLA 482

Query: 482 APVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYIS 522
           APVEEWTVGGT+LT+LMDVERRHGKFKPVIKKAMVEL+G PFKKFASLR+EWA  NRYIS
Sbjct: 483 APVEEWTVGGTALTALMDVERRHGKFKPVIKKAMVELEGAPFKKFASLREEWALKNRYIS 542

BLAST of Cp4.1LG19g08170 vs. ExPASy Swiss-Prot
Match: F4JGR5 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 OS=Arabidopsis thaliana OX=3702 GN=PFP-BETA2 PE=3 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 2.8e-240
Identity = 413/547 (75.50%), Postives = 459/547 (83.91%), Query Frame = 0

Query: 21  SGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSAAGNPDEIAKLFPNLFGQPSA 80
           + RV+SVY+EVQ+SRID  LPLPSVF++ F I+DGPPSS+AG+P+EI KLFPNLFGQPSA
Sbjct: 23  NSRVTSVYSEVQASRIDHTLPLPSVFKTPFKIIDGPPSSSAGHPEEIEKLFPNLFGQPSA 82

Query: 81  KLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDYLQDHVKGSVLYGFKGGPAGI 140
            LVP++SN+  SD+KLKIGVVLSGGQAPGGHNVI GIFDYLQ++ +GS L+GF+GGPAGI
Sbjct: 83  LLVPNQSNEVSSDQKLKIGVVLSGGQAPGGHNVICGIFDYLQEYARGSSLFGFRGGPAGI 142

Query: 141 MKCNYVELTAEYIYPYRNQGGFDMIRSGRDKFETTEQFKQAEETAKKLDLDGIVVIGGDD 200
           MK  Y+ELT+E++YPYRNQGGFDMI SGRDK ET EQFKQAEET  K+DLDG+VVIGGDD
Sbjct: 143 MKGKYIELTSEFVYPYRNQGGFDMICSGRDKIETPEQFKQAEETVTKMDLDGLVVIGGDD 202

Query: 201 SNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPISFGFDTACRIYAEMIGNVMID 260
           SNTNACLLAE+FR+KN+KT VIGCPKTIDGDLK KEVP SFGFDTAC+IY+EMIGNVMID
Sbjct: 203 SNTNACLLAEHFRAKNMKTLVIGCPKTIDGDLKSKEVPTSFGFDTACKIYSEMIGNVMID 262

Query: 261 ARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGE---------------------- 320
           ARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGE                      
Sbjct: 263 ARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVFEKKLTLKNVTDNIVDVIYK 322

Query: 321 ----------------------EVQQLIAELNEILAHEIVDDEGLWKKKLTSQSLELFEL 380
                                 EVQQLI+ELNE+LA   VD+EG WKK L  ++LE+FE 
Sbjct: 323 RAENGYNYGVILVPEGLIDFIPEVQQLISELNEVLAEGNVDEEGQWKKNLKKETLEIFEF 382

Query: 381 LPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKSEGAYNGQFKGQSHFFGY 440
           LPQ IQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK+K+EG Y  +F G+SHFFGY
Sbjct: 383 LPQTIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKKKTEGTYEREFMGKSHFFGY 442

Query: 441 EGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNLAAPVEEWTVGGTSLTSLMDV 500
           EGRCGLPTNFD+TYCYALGYGAG+LLQSGKTGLISSVGNLAAPVEEWTVGGT+LTSLMDV
Sbjct: 443 EGRCGLPTNFDATYCYALGYGAGSLLQSGKTGLISSVGNLAAPVEEWTVGGTALTSLMDV 502

Query: 501 ERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYISPGPIQFVGPASSAINHTLL 524
           ERRHGKFKPVIKKAMVEL+G PFKKFAS R+EWA  NRYISPGPIQF GP S A NHTL+
Sbjct: 503 ERRHGKFKPVIKKAMVELEGAPFKKFASQREEWALKNRYISPGPIQFKGPGSDARNHTLM 562

BLAST of Cp4.1LG19g08170 vs. ExPASy Swiss-Prot
Match: Q9EZ02 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase OS=Spirochaeta thermophila (strain ATCC 49972 / DSM 6192 / RI 19.B1) OX=665571 GN=pfp PE=1 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 3.2e-135
Identity = 256/548 (46.72%), Postives = 344/548 (62.77%), Query Frame = 0

Query: 31  VQSSRIDQNLPLPSVFRSSFTIVD---GPPSSAAGNPDEIAKLFPNLFGQPSAKLVPSKS 90
           +Q +R      LP V +     +    G P+ A  + +++ +LF N +G+P A L  ++ 
Sbjct: 7   LQKARYGYVPKLPPVLQDEIARIQAELGQPTEAVADREDLKRLFANTYGKPIATL--TRG 66

Query: 91  NKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDYLQDHVKGSVLYGFKGGPAGIMKCNYVE 150
           +  ++ ++  +GV+LSGG APGGHNVI G+FD L+   + S L GFKGGP+GI+   ++E
Sbjct: 67  SNPEAGRRRTVGVILSGGPAPGGHNVICGLFDALKKANRESTLIGFKGGPSGILDDEWIE 126

Query: 151 LTAEYIYPYRNQGGFDMIRSGRDKFETTEQFKQAEETAKKLDLDGIVVIGGDDSNTNACL 210
            T   I  YRN GGFD+I SGR K ET EQF +A E AKK  LD +V+IGGDDSNTNA L
Sbjct: 127 FTDSLINQYRNTGGFDIIGSGRTKIETPEQFAKALENAKKHGLDALVIIGGDDSNTNAAL 186

Query: 211 LAENFRSKNLKTRVIGCPKTIDGDLKCKEVPISFGFDTACRIYAEMIGNVMIDARSTGKY 270
           LAE F  +    +VIG PKTIDGDLK + +  SFGFDTA ++YAE+IGN+  DA S+ KY
Sbjct: 187 LAEYFVQQGAPIQVIGIPKTIDGDLKNEYIEASFGFDTATKVYAELIGNIARDAISSRKY 246

Query: 271 YHFVRLMGRAASHITLECALQTHPNITIIGEEVQQ------------------------- 330
           +HF+RLMGR+ASHI LECALQTHPN+ I+ EEV++                         
Sbjct: 247 WHFIRLMGRSASHIALECALQTHPNVCIVSEEVREKNMTLSQIVDQIVDAVVKRAAKGEN 306

Query: 331 -------------------LIAELNEILAHEI-----VDDEG---LW-KKKLTSQSLELF 390
                              LI ELN +LA E      +DD      W K KL+  +  +F
Sbjct: 307 FGVVLVPEGLIEFIPEVGALIDELNTLLAKEAEVFNRIDDPRERISWVKGKLSGNNQHVF 366

Query: 391 ELLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKSEGAYNGQFKGQSHFF 450
             LP+ IQ QL+++RDPHGNVQV++IETEK+LI+MV + L+  K EGAY G+F   +HFF
Sbjct: 367 SSLPETIQAQLLMDRDPHGNVQVSRIETEKLLIEMVSSRLKALKEEGAYKGKFSALNHFF 426

Query: 451 GYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNLAAPVEEWTVGGTSLTSLM 510
           GYE RC  P+NFD+ YCYALG+ A  L+ +G TG I+++ NLA P  EW   G  LT +M
Sbjct: 427 GYEARCAFPSNFDADYCYALGFTAFVLIANGLTGYIAAIKNLARPAVEWKPMGIPLTMMM 486

Query: 511 DVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYISPGPIQFVGPASSAINHT 523
           ++E+RHGK KPVI+KA+V+L+G PFK+FA+ RD WA  + Y+ PG IQ+ GP       T
Sbjct: 487 NMEKRHGKMKPVIRKALVDLEGAPFKRFATQRDAWATESAYVFPGAIQYYGPDDVCNQPT 546

BLAST of Cp4.1LG19g08170 vs. NCBI nr
Match: XP_023518798.1 (pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1015 bits (2624), Expect = 0.0
Identity = 523/567 (92.24%), Postives = 523/567 (92.24%), Query Frame = 0

Query: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSA 60
           MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSA
Sbjct: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMIRSGRDKFETTEQFKQ 180
           LQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMIRSGRDKFETTEQFKQ
Sbjct: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMIRSGRDKFETTEQFKQ 180

Query: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240
           AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240

Query: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 --------------------------------------------QQLIAELNEILAHEIV 360
                                                       QQLIAELNEILAHEIV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGFNYGVILIPEGLIDFIPEVQQLIAELNEILAHEIV 360

Query: 361 DDEGLWKKKLTSQSLELFELLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420
           DDEGLWKKKLTSQSLELFELLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKKKLTSQSLELFELLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420

Query: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480
           RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL
Sbjct: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYI 523
           AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYI 540

BLAST of Cp4.1LG19g08170 vs. NCBI nr
Match: XP_022926964.1 (pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta-like [Cucurbita moschata] >KAG6594681.1 Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026650.1 Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 999 bits (2584), Expect = 0.0
Identity = 516/567 (91.01%), Postives = 518/567 (91.36%), Query Frame = 0

Query: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSA 60
           MPSS VSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSA
Sbjct: 1   MPSSIVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMIRSGRDKFETTEQFKQ 180
           LQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMI SGRDKFETTEQFKQ
Sbjct: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMICSGRDKFETTEQFKQ 180

Query: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240
           AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240

Query: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 --------------------------------------------QQLIAELNEILAHEIV 360
                                                       QQLIAELNEILAHEIV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGFNYGVILIPEGLIDFIPEVQQLIAELNEILAHEIV 360

Query: 361 DDEGLWKKKLTSQSLELFELLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420
           DDEGLWKKKLTSQSLELFELLP+AIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420

Query: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480
           RK E AYN QFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL
Sbjct: 421 RKRESAYNRQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYI 523
           AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYI 540

BLAST of Cp4.1LG19g08170 vs. NCBI nr
Match: XP_023003713.1 (pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta-like [Cucurbita maxima])

HSP 1 Score: 986 bits (2550), Expect = 0.0
Identity = 507/567 (89.42%), Postives = 515/567 (90.83%), Query Frame = 0

Query: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSA 60
           MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRID  LPLPSV RSSFTIVDGPPSSA
Sbjct: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLV S SNKAQ DKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMIRSGRDKFETTEQFKQ 180
           LQDHVKGSVLYGFKGGPAGIMKCNYVEL +EYI+PYRNQGGFDMI SGRDKFETTEQFKQ
Sbjct: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQ 180

Query: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240
           AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240

Query: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIY+EMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 --------------------------------------------QQLIAELNEILAHEIV 360
                                                       QQLIAELNEILAHEIV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIV 360

Query: 361 DDEGLWKKKLTSQSLELFELLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420
           DDEGLWKKKLTSQSLELFELLP+AIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420

Query: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480
           RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAG+LLQSGKTGLISSVGNL
Sbjct: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYI 523
           AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVE+DGTPFKK+ASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYI 540

BLAST of Cp4.1LG19g08170 vs. NCBI nr
Match: XP_022950848.1 (pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta [Cucurbita moschata])

HSP 1 Score: 959 bits (2478), Expect = 0.0
Identity = 491/567 (86.60%), Postives = 504/567 (88.89%), Query Frame = 0

Query: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSA 60
           M  SF+SNGEAAV+S+PA V+GRVSSVY+EVQSSRID NLPLPSV RSSFTIVDGPPSSA
Sbjct: 1   MSISFISNGEAAVRSNPAPVTGRVSSVYSEVQSSRIDHNLPLPSVLRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLVPS SNK Q DKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPSDSNKVQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMIRSGRDKFETTEQFKQ 180
           LQDHVKGSVLYGF+GGPAGIMKC YVELT+EYIYPYRNQGGFDMI SGRDK ET EQFKQ
Sbjct: 121 LQDHVKGSVLYGFRGGPAGIMKCKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180

Query: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240
           AEETAKKLDLDGIVVIGGDDSNTNACLLAENFR KNLKTRVIGCPKTIDGDLKCKEVP S
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240

Query: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 --------------------------------------------QQLIAELNEILAHEIV 360
                                                       QQLI+ELNEILAH+IV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGYNYGVILIPEGLIDFIPEVQQLISELNEILAHDIV 360

Query: 361 DDEGLWKKKLTSQSLELFELLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420
           DDEGLWK+KLTSQSLELFE LPQAIQEQLMLERDPHGNVQVA+IETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKRKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420

Query: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480
           RKSEGAY  QFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL
Sbjct: 421 RKSEGAYKAQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYI 523
           AAPVEEWTVGGT+LTSLMDVERRHGKFKPVIKKAMVELDG PFKKFASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540

BLAST of Cp4.1LG19g08170 vs. NCBI nr
Match: KAG7033973.1 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 956 bits (2471), Expect = 0.0
Identity = 490/567 (86.42%), Postives = 503/567 (88.71%), Query Frame = 0

Query: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSA 60
           M  SF+SNGEAAV+S+PA V+GRVSSVY+EVQSSRID NLPLPSV RSSFTIVDGPPSSA
Sbjct: 1   MSISFISNGEAAVRSNPAPVTGRVSSVYSEVQSSRIDHNLPLPSVLRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLVPS SNK Q  KKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPSDSNKVQPGKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMIRSGRDKFETTEQFKQ 180
           LQDHVKGSVLYGF+GGPAGIMKC YVELT+EYIYPYRNQGGFDMI SGRDK ET EQFKQ
Sbjct: 121 LQDHVKGSVLYGFRGGPAGIMKCKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180

Query: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240
           AEETAKKLDLDGIVVIGGDDSNTNACLLAENFR KNLKTRVIGCPKTIDGDLKCKEVP S
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240

Query: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 --------------------------------------------QQLIAELNEILAHEIV 360
                                                       QQLI+ELNEILAH+IV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGYNYGVILIPEGLIDFIPEVQQLISELNEILAHDIV 360

Query: 361 DDEGLWKKKLTSQSLELFELLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420
           DDEGLWK+KLTSQSLELFE LPQAIQEQLMLERDPHGNVQVA+IETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKRKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420

Query: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480
           RKSEGAY  QFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL
Sbjct: 421 RKSEGAYKAQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYI 523
           AAPVEEWTVGGT+LTSLMDVERRHGKFKPVIKKAMVELDG PFKKFASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540

BLAST of Cp4.1LG19g08170 vs. ExPASy TrEMBL
Match: A0A6J1EGM8 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111433922 PE=3 SV=1)

HSP 1 Score: 999 bits (2584), Expect = 0.0
Identity = 516/567 (91.01%), Postives = 518/567 (91.36%), Query Frame = 0

Query: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSA 60
           MPSS VSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSA
Sbjct: 1   MPSSIVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMIRSGRDKFETTEQFKQ 180
           LQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMI SGRDKFETTEQFKQ
Sbjct: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMICSGRDKFETTEQFKQ 180

Query: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240
           AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240

Query: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 --------------------------------------------QQLIAELNEILAHEIV 360
                                                       QQLIAELNEILAHEIV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGFNYGVILIPEGLIDFIPEVQQLIAELNEILAHEIV 360

Query: 361 DDEGLWKKKLTSQSLELFELLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420
           DDEGLWKKKLTSQSLELFELLP+AIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420

Query: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480
           RK E AYN QFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL
Sbjct: 421 RKRESAYNRQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYI 523
           AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYI 540

BLAST of Cp4.1LG19g08170 vs. ExPASy TrEMBL
Match: A0A6J1KXF5 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111497221 PE=3 SV=1)

HSP 1 Score: 986 bits (2550), Expect = 0.0
Identity = 507/567 (89.42%), Postives = 515/567 (90.83%), Query Frame = 0

Query: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSA 60
           MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRID  LPLPSV RSSFTIVDGPPSSA
Sbjct: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDHTLPLPSVLRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLV S SNKAQ DKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVQSNSNKAQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMIRSGRDKFETTEQFKQ 180
           LQDHVKGSVLYGFKGGPAGIMKCNYVEL +EYI+PYRNQGGFDMI SGRDKFETTEQFKQ
Sbjct: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELNSEYIHPYRNQGGFDMICSGRDKFETTEQFKQ 180

Query: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240
           AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240

Query: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIY+EMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 --------------------------------------------QQLIAELNEILAHEIV 360
                                                       QQLIAELNEILAHEIV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGFNYGMILIPEGLIDFIPEVQQLIAELNEILAHEIV 360

Query: 361 DDEGLWKKKLTSQSLELFELLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420
           DDEGLWKKKLTSQSLELFELLP+AIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKKKLTSQSLELFELLPRAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420

Query: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480
           RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAG+LLQSGKTGLISSVGNL
Sbjct: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGSLLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYI 523
           AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVE+DGTPFKK+ASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVEIDGTPFKKYASLRDEWAFNNRYI 540

BLAST of Cp4.1LG19g08170 vs. ExPASy TrEMBL
Match: A0A6J1GG11 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111453824 PE=3 SV=1)

HSP 1 Score: 959 bits (2478), Expect = 0.0
Identity = 491/567 (86.60%), Postives = 504/567 (88.89%), Query Frame = 0

Query: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSA 60
           M  SF+SNGEAAV+S+PA V+GRVSSVY+EVQSSRID NLPLPSV RSSFTIVDGPPSSA
Sbjct: 1   MSISFISNGEAAVRSNPAPVTGRVSSVYSEVQSSRIDHNLPLPSVLRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLVPS SNK Q DKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPSDSNKVQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMIRSGRDKFETTEQFKQ 180
           LQDHVKGSVLYGF+GGPAGIMKC YVELT+EYIYPYRNQGGFDMI SGRDK ET EQFKQ
Sbjct: 121 LQDHVKGSVLYGFRGGPAGIMKCKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180

Query: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240
           AEETAKKLDLDGIVVIGGDDSNTNACLLAENFR KNLKTRVIGCPKTIDGDLKCKEVP S
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240

Query: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 --------------------------------------------QQLIAELNEILAHEIV 360
                                                       QQLI+ELNEILAH+IV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGYNYGVILIPEGLIDFIPEVQQLISELNEILAHDIV 360

Query: 361 DDEGLWKKKLTSQSLELFELLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420
           DDEGLWK+KLTSQSLELFE LPQAIQEQLMLERDPHGNVQVA+IETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKRKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420

Query: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480
           RKSEGAY  QFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL
Sbjct: 421 RKSEGAYKAQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYI 523
           AAPVEEWTVGGT+LTSLMDVERRHGKFKPVIKKAMVELDG PFKKFASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540

BLAST of Cp4.1LG19g08170 vs. ExPASy TrEMBL
Match: A0A6J1IR64 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111478001 PE=3 SV=1)

HSP 1 Score: 951 bits (2459), Expect = 0.0
Identity = 487/567 (85.89%), Postives = 502/567 (88.54%), Query Frame = 0

Query: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSA 60
           M  SF+SNGEAAV+S+ A V+GRVSSVY+EVQSSRID NLPLPSV RSSFTIVDGPPSSA
Sbjct: 1   MSISFISNGEAAVRSNHAPVTGRVSSVYSEVQSSRIDHNLPLPSVLRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLVPS SNK Q DKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPSDSNKVQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMIRSGRDKFETTEQFKQ 180
           LQDHVK SVLYGF+GGPAGIMKC YVELT+EYIYPYRNQGGFDMI SGRDK ET EQFKQ
Sbjct: 121 LQDHVKSSVLYGFRGGPAGIMKCKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180

Query: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240
           AEETAKKLDLDGIVVIGGDDSNTNACLLAENFR KNLKTRVIGCPKTIDGDLKCKEVP S
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240

Query: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIYAEMIGNVM+DARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMVDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 --------------------------------------------QQLIAELNEILAHEIV 360
                                                       QQLI+ELNEILAH+IV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGYNYGVILIPEGLIDFIPEVQQLISELNEILAHDIV 360

Query: 361 DDEGLWKKKLTSQSLELFELLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420
           DDEGLWK+KLTSQSLELFE LPQAIQEQLMLERDPHGNVQVA+IETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKRKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420

Query: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480
           RK+EGAY  QFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL
Sbjct: 421 RKNEGAYKAQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYI 523
           AAPVEEWTVGGT+LTSLMDVERRHGKFKPVIKKAMVELDG PFKKFASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540

BLAST of Cp4.1LG19g08170 vs. ExPASy TrEMBL
Match: A0A0A0KH95 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucumis sativus OX=3659 GN=PFP-BETA PE=3 SV=1)

HSP 1 Score: 949 bits (2452), Expect = 0.0
Identity = 484/567 (85.36%), Postives = 500/567 (88.18%), Query Frame = 0

Query: 1   MPSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSA 60
           M  SF+SNGEAAVKSS   +SGRVSSVY+EVQSSRI+ NLPLPSV RS FTIVDGPPSSA
Sbjct: 1   MSISFISNGEAAVKSSSPPLSGRVSSVYSEVQSSRINHNLPLPSVLRSPFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLVPS SNK Q DKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPSDSNKGQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMIRSGRDKFETTEQFKQ 180
           LQDH KGSVLYGF+GGPAGIMKC Y+ELT++YIYPYRNQGGFDMI SGRDK ET EQFKQ
Sbjct: 121 LQDHAKGSVLYGFRGGPAGIMKCKYLELTSDYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180

Query: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPIS 240
           AEETAKKLDLDG+VVIGGDDSNTNACLLAENFR KNLKTRVIGCPKTIDGDLKCKEVP S
Sbjct: 181 AEETAKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240

Query: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 --------------------------------------------QQLIAELNEILAHEIV 360
                                                       QQLIAELNEILAH++V
Sbjct: 301 YAQKQTLKSVTDYIVDVVCKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDVV 360

Query: 361 DDEGLWKKKLTSQSLELFELLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK 420
           DDEGLWKKKLTSQSLELFE LPQAIQEQLMLERDPHGNVQVA+IETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420

Query: 421 RKSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480
           RKSEGAY GQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL
Sbjct: 421 RKSEGAYKGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYI 523
           AAPVEEWTVGGT+LTSLMDVERRHGKFKPVIKKAMVELDG PFKKFASLRD+WAFNNRYI
Sbjct: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDDWAFNNRYI 540

BLAST of Cp4.1LG19g08170 vs. TAIR 10
Match: AT1G12000.1 (Phosphofructokinase family protein )

HSP 1 Score: 846.7 bits (2186), Expect = 1.0e-245
Identity = 431/564 (76.42%), Postives = 465/564 (82.45%), Query Frame = 0

Query: 2   PSSFVSNGEAAVKSSPAAVSGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSAA 61
           P+  V+    AV S   A +   +SVY+EVQSSRI+  LPLPSV + +F IV+GP SSAA
Sbjct: 3   PALAVTRDLTAVGSPENAPAKGRASVYSEVQSSRINNTLPLPSVLKGAFKIVEGPASSAA 62

Query: 62  GNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDYL 121
           GNPDEIAKLFP L+GQPS  +VP + +   S  KLKIGVVLSGGQAPGGHNVISG+FDYL
Sbjct: 63  GNPDEIAKLFPGLYGQPSVAVVPDQ-DAPSSAPKLKIGVVLSGGQAPGGHNVISGLFDYL 122

Query: 122 QDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMIRSGRDKFETTEQFKQA 181
           Q+  KGS  YGFKGGPAGIMKC YVEL AEYI PYRNQGGFDMI SGRDK ET +QFKQA
Sbjct: 123 QERAKGSTFYGFKGGPAGIMKCKYVELNAEYIQPYRNQGGFDMICSGRDKIETPDQFKQA 182

Query: 182 EETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPISF 241
           EETAKKLDLDG+VVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVP SF
Sbjct: 183 EETAKKLDLDGLVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPTSF 242

Query: 242 GFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGE--- 301
           GFDTAC+IY+EMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGE   
Sbjct: 243 GFDTACKIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVS 302

Query: 302 -----------------------------------------EVQQLIAELNEILAHEIVD 361
                                                    EVQ+LIAELNEILA+E+VD
Sbjct: 303 AQKQTLKNVTDYMVDVICKRAELGYNYGVILIPEGLIDFIPEVQELIAELNEILANEVVD 362

Query: 362 DEGLWKKKLTSQSLELFELLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKR 421
           + GLWKKKLT QSL+LF+LLP+AIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKR
Sbjct: 363 ENGLWKKKLTEQSLKLFDLLPEAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKR 422

Query: 422 KSEGAYNGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNLA 481
           K  GAY GQF GQSHFFGYEGRCGLPTNFD+TYCYALGYGAG LL SGKTGLISSVGNLA
Sbjct: 423 KQAGAYKGQFMGQSHFFGYEGRCGLPTNFDATYCYALGYGAGVLLNSGKTGLISSVGNLA 482

Query: 482 APVEEWTVGGTSLTSLMDVERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYIS 522
           APVEEWTVGGT+LT+LMDVERRHGKFKPVIKKAMVEL+G PFKKFASLR+EWA  NRYIS
Sbjct: 483 APVEEWTVGGTALTALMDVERRHGKFKPVIKKAMVELEGAPFKKFASLREEWALKNRYIS 542

BLAST of Cp4.1LG19g08170 vs. TAIR 10
Match: AT4G04040.1 (Phosphofructokinase family protein )

HSP 1 Score: 832.4 bits (2149), Expect = 2.0e-241
Identity = 413/547 (75.50%), Postives = 459/547 (83.91%), Query Frame = 0

Query: 21  SGRVSSVYAEVQSSRIDQNLPLPSVFRSSFTIVDGPPSSAAGNPDEIAKLFPNLFGQPSA 80
           + RV+SVY+EVQ+SRID  LPLPSVF++ F I+DGPPSS+AG+P+EI KLFPNLFGQPSA
Sbjct: 23  NSRVTSVYSEVQASRIDHTLPLPSVFKTPFKIIDGPPSSSAGHPEEIEKLFPNLFGQPSA 82

Query: 81  KLVPSKSNKAQSDKKLKIGVVLSGGQAPGGHNVISGIFDYLQDHVKGSVLYGFKGGPAGI 140
            LVP++SN+  SD+KLKIGVVLSGGQAPGGHNVI GIFDYLQ++ +GS L+GF+GGPAGI
Sbjct: 83  LLVPNQSNEVSSDQKLKIGVVLSGGQAPGGHNVICGIFDYLQEYARGSSLFGFRGGPAGI 142

Query: 141 MKCNYVELTAEYIYPYRNQGGFDMIRSGRDKFETTEQFKQAEETAKKLDLDGIVVIGGDD 200
           MK  Y+ELT+E++YPYRNQGGFDMI SGRDK ET EQFKQAEET  K+DLDG+VVIGGDD
Sbjct: 143 MKGKYIELTSEFVYPYRNQGGFDMICSGRDKIETPEQFKQAEETVTKMDLDGLVVIGGDD 202

Query: 201 SNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPISFGFDTACRIYAEMIGNVMID 260
           SNTNACLLAE+FR+KN+KT VIGCPKTIDGDLK KEVP SFGFDTAC+IY+EMIGNVMID
Sbjct: 203 SNTNACLLAEHFRAKNMKTLVIGCPKTIDGDLKSKEVPTSFGFDTACKIYSEMIGNVMID 262

Query: 261 ARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGE---------------------- 320
           ARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGE                      
Sbjct: 263 ARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVFEKKLTLKNVTDNIVDVIYK 322

Query: 321 ----------------------EVQQLIAELNEILAHEIVDDEGLWKKKLTSQSLELFEL 380
                                 EVQQLI+ELNE+LA   VD+EG WKK L  ++LE+FE 
Sbjct: 323 RAENGYNYGVILVPEGLIDFIPEVQQLISELNEVLAEGNVDEEGQWKKNLKKETLEIFEF 382

Query: 381 LPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKSEGAYNGQFKGQSHFFGY 440
           LPQ IQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEK+K+EG Y  +F G+SHFFGY
Sbjct: 383 LPQTIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKKKTEGTYEREFMGKSHFFGY 442

Query: 441 EGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNLAAPVEEWTVGGTSLTSLMDV 500
           EGRCGLPTNFD+TYCYALGYGAG+LLQSGKTGLISSVGNLAAPVEEWTVGGT+LTSLMDV
Sbjct: 443 EGRCGLPTNFDATYCYALGYGAGSLLQSGKTGLISSVGNLAAPVEEWTVGGTALTSLMDV 502

Query: 501 ERRHGKFKPVIKKAMVELDGTPFKKFASLRDEWAFNNRYISPGPIQFVGPASSAINHTLL 524
           ERRHGKFKPVIKKAMVEL+G PFKKFAS R+EWA  NRYISPGPIQF GP S A NHTL+
Sbjct: 503 ERRHGKFKPVIKKAMVELEGAPFKKFASQREEWALKNRYISPGPIQFKGPGSDARNHTLM 562

BLAST of Cp4.1LG19g08170 vs. TAIR 10
Match: AT1G76550.1 (Phosphofructokinase family protein )

HSP 1 Score: 328.6 bits (841), Expect = 9.4e-90
Identity = 191/520 (36.73%), Postives = 285/520 (54.81%), Query Frame = 0

Query: 54  DGPPSSAAGNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSD-----KKLKIGVVLSGGQAP 113
           DG   + AG+   IA+ FP+  GQP A  + + +  A +         ++G+V  G QAP
Sbjct: 40  DGTTVAKAGDAHIIARAFPHTLGQPLAHFLRATAKVADAQIITEHPVKRVGIVFCGRQAP 99

Query: 114 GGHNVISGIFDYLQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMIRSG 173
           GGHNV+ G+++ L+ H   + L GF GG  G+     +E+T E +  Y+NQGG+DM+   
Sbjct: 100 GGHNVVWGLYEALKVHNAKNTLLGFLGGSEGLFAQKTLEITDEVLQTYKNQGGYDMLGRT 159

Query: 174 RDKFETTEQFKQAEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTI 233
           +D+  TTEQ   A +    L LD +V+IGG  SNT+A  LAE F      T+V+G P TI
Sbjct: 160 KDQIRTTEQVNAALKACTDLKLDSLVIIGGVTSNTDAAHLAEFFAEAKCSTKVVGVPVTI 219

Query: 234 DGDLKCKEVPISFGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQ 293
           +GDLK + V  + GFDT C++ +++I N+  DA S  KYY+FVRLMGR  SH+ LEC LQ
Sbjct: 220 NGDLKNQFVEANVGFDTTCKVNSQLISNICTDALSAEKYYYFVRLMGRKHSHVALECTLQ 279

Query: 294 THPNITIIGEEV----------------------------------QQLIAELNEILAHE 353
           +HPN+ I+GEEV                                  + L+  + E+ A  
Sbjct: 280 SHPNMVILGEEVTASKLTIFDIIKQICDAVQARAEQDKNHGVILIPEGLVESIPELYA-L 339

Query: 354 IVDDEGLWKK---------KLTSQSLELFELLPQAIQEQLMLERDPHGNVQVAKIETEKM 413
           + +  GL K+         +L+S S  LFE LP  I++QL+L  +   + Q+++IETEK+
Sbjct: 340 LKEIHGLLKEGVQVDNISTQLSSWSSALFEFLPPFIKKQLLLHPESDDSAQLSQIETEKL 399

Query: 414 LIQMVETELEKRKSEGAYNG-QFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQS 473
           L  +VETE+ KR  EG Y G +F    HFFGY+ R  LP+ FD  Y Y LG+    +L +
Sbjct: 400 LAYLVETEMNKRLKEGTYKGKKFNAICHFFGYQARGSLPSKFDCDYAYVLGHVCYHILAA 459

Query: 474 GKTGLISSVGNLAAPVEEWTVGGTSLTSLMDVER---RHGKF---KPVIKKAMVELDGTP 519
           G  G +++V NL +PV +W  G   ++++M V+R     G     +PVI  A V+L G  
Sbjct: 460 GLNGYMATVTNLKSPVNKWKCGAAPISAMMTVKRWSQNSGSTTIGRPVIHPASVDLKGKA 519

BLAST of Cp4.1LG19g08170 vs. TAIR 10
Match: AT1G20950.1 (Phosphofructokinase family protein )

HSP 1 Score: 324.3 bits (830), Expect = 1.8e-88
Identity = 188/522 (36.02%), Postives = 286/522 (54.79%), Query Frame = 0

Query: 54  DGPPSSAAGNPDEIAKLFPNLFGQPSAKLVPSKSNKAQSD-----KKLKIGVVLSGGQAP 113
           DG   + A +   +A+ FP+  GQP A  +   +    +        +++G+V  G QAP
Sbjct: 40  DGTTVAEAADSHTMARAFPHTLGQPLAHFLRETAQVPDAHIITELPSVRVGIVFCGRQAP 99

Query: 114 GGHNVISGIFDYLQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQGGFDMIRSG 173
           GGHNVI G+F+ L+ H   S L GF GG  G+     +E+T + +  Y+NQGG+D++   
Sbjct: 100 GGHNVIWGLFEALKVHNAKSTLLGFLGGSEGLFAQKTLEITDDILQTYKNQGGYDLLGRT 159

Query: 174 RDKFETTEQFKQAEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTI 233
           +D+ +TTEQ   A +    L LDG+V+IGG  SNT+A  LAE F +    T+V+G P T 
Sbjct: 160 KDQIKTTEQVNAALKACTDLKLDGLVIIGGVISNTDAAHLAEFFAAAKCSTKVVGVPVTT 219

Query: 234 DGDLKCKEVPISFGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQ 293
           +GDLK + V  + GFDT C++ +++I N   DA S  KYY+F+RLMGR  SH+ LEC LQ
Sbjct: 220 NGDLKNQFVEANVGFDTICKVNSQLISNACTDALSAEKYYYFIRLMGRKHSHVALECTLQ 279

Query: 294 THPNITIIGEEV----------------------------------QQLIAELNEILA-- 353
           +HPN+ I+GEEV                                  + LI  + E+ A  
Sbjct: 280 SHPNMVILGEEVAASKLTIFDIAKQICDAVQARAVEDKNHGVILIPEGLIVSIPEVYALL 339

Query: 354 ---HEIVDDEGLWKKKLTSQ----SLELFELLPQAIQEQLMLERDPHGNVQVAKIETEKM 413
              H ++  +G+   K+++Q    S  LFE LP  I++QL+L  +   + Q+++IETEK+
Sbjct: 340 KEIHGLL-RQGVSADKISTQLSPWSSALFEFLPPFIKKQLLLHPESDDSAQLSQIETEKL 399

Query: 414 LIQMVETELEKRKSEGAYNG-QFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQS 473
           L  +VETE+ KR  EG Y G +F    HFFGY+ R  LP+ FD  Y Y LG+    +L +
Sbjct: 400 LAYLVETEMNKRLKEGTYKGKKFNAICHFFGYQARGSLPSKFDCDYAYVLGHICYHILAA 459

Query: 474 GKTGLISSVGNLAAPVEEWTVGGTSLTSLMDVER--RHGKF------KPVIKKAMVELDG 519
           G  G +++V NL +PV +W  G T +T++M V+   +   +      +P I  AMV+L G
Sbjct: 460 GLNGYMATVTNLKSPVNKWKCGATPITAMMTVKHWSQDASYTLTSIGRPAIHPAMVDLKG 519

BLAST of Cp4.1LG19g08170 vs. TAIR 10
Match: AT5G56630.1 (phosphofructokinase 7 )

HSP 1 Score: 84.3 bits (207), Expect = 3.1e-16
Identity = 54/188 (28.72%), Postives = 86/188 (45.74%), Query Frame = 0

Query: 100 VVLSGGQAPGGHNVISGIFDYLQDHVKGSVLYGFKGGPAGIMKCNYVELTAEYIYPYRNQ 159
           +V  GG  PG + VI  +   L        + G  GG  G    N + L ++ +     +
Sbjct: 96  IVTCGGLCPGLNTVIREVVSSLSYMYGVKRILGIDGGYRGFYAKNTIPLNSKVVNDIHKR 155

Query: 160 GGFDMIRSGRDKFETTEQFKQAEETAKKLDLDGIVVIGGDDSNTNACLLAENFRSKNLKT 219
           GG  +I + R   +T +     ++      ++ + +IGGD +   A ++ E  R + LK 
Sbjct: 156 GG-TIIGTSRGGHDTNKIVDSIQDRG----INQVYIIGGDGTQRGASVIFEEIRRRRLKV 215

Query: 220 RVIGCPKTIDGDLKCKEVPISFGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAAS 279
            V+G PKTID D+    +  SFGFDTA       I    ++A S      FV+LMGR + 
Sbjct: 216 AVVGIPKTIDNDIPV--IDKSFGFDTAVEEAQRAINAAHVEAESNENGIGFVKLMGRYSG 275

Query: 280 HITLECAL 288
           +I +   L
Sbjct: 276 YIAMYATL 276

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q411412.2e-24878.79Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Ricinus... [more]
P213436.5e-24575.71Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Solanum... [more]
Q8W4M51.4e-24476.42Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 OS=Arabi... [more]
F4JGR52.8e-24075.50Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 OS=Arabi... [more]
Q9EZ023.2e-13546.72Pyrophosphate--fructose 6-phosphate 1-phosphotransferase OS=Spirochaeta thermoph... [more]
Match NameE-valueIdentityDescription
XP_023518798.10.092.24pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta-like [Cucu... [more]
XP_022926964.10.091.01pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta-like [Cucu... [more]
XP_023003713.10.089.42pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta-like [Cucu... [more]
XP_022950848.10.086.60pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta [Cucurbita... [more]
KAG7033973.10.086.42Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta [Cucurbita... [more]
Match NameE-valueIdentityDescription
A0A6J1EGM80.091.01Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbi... [more]
A0A6J1KXF50.089.42Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbi... [more]
A0A6J1GG110.086.60Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbi... [more]
A0A6J1IR640.085.89Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbi... [more]
A0A0A0KH950.085.36Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucumis... [more]
Match NameE-valueIdentityDescription
AT1G12000.11.0e-24576.42Phosphofructokinase family protein [more]
AT4G04040.12.0e-24175.50Phosphofructokinase family protein [more]
AT1G76550.19.4e-9036.73Phosphofructokinase family protein [more]
AT1G20950.11.8e-8836.02Phosphofructokinase family protein [more]
AT5G56630.13.1e-1628.72phosphofructokinase 7 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022953ATP-dependent 6-phosphofructokinasePRINTSPR00476PHFRCTKINASEcoord: 100..119
score: 32.56
coord: 189..205
score: 46.91
coord: 222..239
score: 34.9
coord: 281..298
score: 24.5
IPR035966Phosphofructokinase superfamilyGENE3D3.40.50.460Phosphofructokinase domaincoord: 364..507
e-value: 6.0E-86
score: 290.9
IPR035966Phosphofructokinase superfamilySUPERFAMILY53784Phosphofructokinasecoord: 33..518
NoneNo IPR availableGENE3D3.40.50.450coord: 31..291
e-value: 4.4E-84
score: 283.8
coord: 403..472
e-value: 6.0E-86
score: 290.9
NoneNo IPR availableGENE3D1.10.10.480Phosphofructokinase; domain 3coord: 299..363
e-value: 6.0E-86
score: 290.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availablePANTHERPTHR43650PYROPHOSPHATE--FRUCTOSE 6-PHOSPHATE 1-PHOSPHOTRANSFERASEcoord: 299..523
NoneNo IPR availablePANTHERPTHR43650:SF16PYROPHOSPHATE--FRUCTOSE 6-PHOSPHATE 1-PHOSPHOTRANSFERASE SUBUNIT BETA 2coord: 15..300
NoneNo IPR availablePANTHERPTHR43650:SF16PYROPHOSPHATE--FRUCTOSE 6-PHOSPHATE 1-PHOSPHOTRANSFERASE SUBUNIT BETA 2coord: 299..523
NoneNo IPR availablePANTHERPTHR43650PYROPHOSPHATE--FRUCTOSE 6-PHOSPHATE 1-PHOSPHOTRANSFERASEcoord: 15..300
IPR000023Phosphofructokinase domainPFAMPF00365PFKcoord: 97..418
e-value: 6.9E-37
score: 127.3
IPR011183Pyrophosphate-dependent phosphofructokinase PfpBPIRSFPIRSF005677PPi_PFK_PfpBcoord: 21..302
e-value: 3.4E-135
score: 449.5
coord: 299..523
e-value: 1.2E-94
score: 315.6
IPR011183Pyrophosphate-dependent phosphofructokinase PfpBHAMAPMF_01980Phosphofructokinase_II_Longcoord: 27..521
score: 36.593315

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG19g08170.1Cp4.1LG19g08170.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046835 carbohydrate phosphorylation
biological_process GO:0006002 fructose 6-phosphate metabolic process
biological_process GO:0061615 glycolytic process through fructose-6-phosphate
biological_process GO:0015979 photosynthesis
biological_process GO:0009749 response to glucose
biological_process GO:0006096 glycolytic process
cellular_component GO:0005829 cytosol
molecular_function GO:0003872 6-phosphofructokinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0047334 diphosphate-fructose-6-phosphate 1-phosphotransferase activity
molecular_function GO:0046872 metal ion binding