Cp4.1LG19g05720 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG19g05720
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionDihydroceramide fatty acyl 2-hydroxylase
LocationCp4.1LG19: 7933401 .. 7936846 (+)
RNA-Seq ExpressionCp4.1LG19g05720
SyntenyCp4.1LG19g05720
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TATGGGTGAATCTGAAAATGGTATTATGGGTGAATCTGAAAATGGTATTATGGGTGAATCTGAAAATGGTATTATGGGTGAATCTGAAAATGGTATGACAAAAGGTTTAAGGCGGCAATCATGAGCGGCCAAAATGCGTTACACAGCCTGACTCATTTCATTTCATTGAGCGGTGCAAATCCCTCTATTCCCCTTCAACATATAAACGCCACATTGCCCTTCAAATTCTCCAACAACTCACCACCATTTAACCAATCCATCAACCCCATTTTCGAGCCTTTGCTGCCTCTGCCTTTCGTTTTCCTTTCCAATCCACTCCTTCTGCATTCCGCTTTACTGCCCTCAGGTAATTATCATTCCCCTCTTTAATGCCCTTCACTTCTCCTGCGTTTTTGTTTTCTGCTCTTATTAGATCTGTTGTTTTCCCTCCTATTTGTGTTTATTTGTGTTTATTTGTGTTTTGGACGCGGTCGTCAATTGTGGCTTCATTGTTGTTTAGATTCTGGGACTGGGAATGGCAATGAAGAGGCCAATGTAGAATGAAATCGCGAGGTTTTTATTTATTGGTTATATTGTTGTTTAGGGCAACGCATGTGTTTTGGTTCTTCTGGTGATCAACTTCGTACTGTAATACCTTTTACGTTTGCTCCAATAATGCTTAACGTTGAGCATCAAATTATGATTGATATTGATATTTTGTGGTTTTTATGCCTTTTAACCAACCATGTTTCGGGCCACTGGAATTTACTGTAGATCTGAACAAACGATGGTTGCACAGGAATTTACTGTAGATCTTAACAAACCACTTGTGTTCCAGGTAAGATCACCATAAGTTCCTTATAAATTGAAAGGAAAATTGGTTAGTTAGTTATGTTTGTACTCTCATAGGTTGGGCATCTTGGAGAAGCATATCAAGACTGGGTTCACCGCCCTATTGTTAGCAAGGAAAGGCCTCGATTCTTCAAAAGCGACTTCTGGGAGGTGATGATTTCTAGTTTTGAGGACTACAAGATTTGATTAATAGTGTTTTTGATATGTTTGAATGTGGAAACAGTTCTGTACACGCACAGCATGGTGGGTGGTTCCACTTATATGGCTGCCAGTGGTCTGTTGGTTCATTTCTATGTCTATGAACATGGGGCACACACTTGGTCAGATATCATTCATGGTCATCTTCGGCATTTATATTTGGACATTGATCGAATACATAGTGCATCGTTTTCTATTCCATATTGAAACCAGTAGTTATTGGTTAGTGTCTCTCTTCTTCTGTACTGTTGTTATTCTAATTTGGGAATATGTTAACAATAACATTGAACATATTCATGGTTTAGGGGGAATACCATGCATTATCTTATTCATGGTTGCCACCACAAGCACCCAATGGATAGTCTACGACTTGTTTTTCCTCCTACTGCCACAGCTGTTCTGATAGTTCCAGTATGTTCACTCTTTCTTGGCTTTGAAATGAGCAAGCAAGCAATCAATCAAAGACGTTTATAATCGTTGTCAGCCAGCTGATTGTATATTTTCTTTGCATCCATCGTTGACACAGATATGGAACTTGGTTAAACTGTTTTTCCCAACATCTATGGCGCCTGCTCTGTTTGGAGGTGGTCTTTTGGGCTATGTGATGTATGACGTTACCCATTACTACTTGCATCATGGCCAACCCACCGGAGAAATGCCGAAACATTTGAAGGTAATACGTTGATTCTATTCAATCAAATACGTTCTATTCAATCAAATAATGAAATGGGGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGCAGAAATATCACATGAATCATCACTTCCGCATCATGAATAAAGGCTTCGGTGTCACTTCATCACTTTGGGACAGAGCGTTTGGAACACTGCCTGCGTCAAAAGTAGCTGAGAAGACTAGATAGCGAAGTAGTAGTGCTCTCAAGGGAAACTAGACCATTTTTGTCTTCTATTCCTTGTTTCTCACTGTATTTGTTCCATCATCATATTCCAAGGATGAAAGGTTTTATATAGGAAGATTTGATCTAGTCTTAATAATGGAAGATTCGTCGGTGCCCCCACATTTTTGTTATTATTATTAGTTCACCACCTCCAGGCTGAGCAAGCCATATCTGTCGGCTAACTTCTTTTCTCAGCAATTAACTTGTCAATAGATTAAGAGTTGAATGTAAAATCTTGATGGGATTCAAGTTTACTTATTAAAATCTATAATATTTTAAAGAATAATTTTTTGGTGGTCAGCCTGCTAGAACATGTCCATGTTCTTCATTCATTCACAAGAGAATGTGACGAGTTGGGATACTCTGTTCATTTCTGAAGAAATTTTGGGGATGAACCCTGGTCTTCACGTCCACCAATCTCTCAAAGAAATTCCCTTTGAAATACTTCTCCCCATAAACCCTTCCTTCTTCCACGCTCCAATGCCCGCTACTCCCAACGTCAATGTCTCTGTAATTAAGAAAGGCTTCCCTTGGATTCTTGGTTACGTATGGAGTCATCTCTTCGTACATTCTTCTCATCACGTTTATCCTCTTATTCTCTTCCTCCTTCCCTCCTTATAAACAGATTCTCATCCAGCTTCTCTATAACTTCCTGCCATTGCAAAACGACATCTGTTGCTCCTTCTTCAATTGTTCTGTTCACTTTAAAAACCGTCACTGTTGATGGGACTGGAACTAATTTGATCTTCCATGACAGAATGACCCCAAAGCTCCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCTAATGGCGCAGAATAAATCTTCCCCCACTTGTTGTCTGTTTAGAGTCTTCCCTTTCACATTGACAATCTGAGCATCCAAGATGTTGTCTGTGGAGAGCCCAAACTTCCTCATCAAATTCCCATACCCACCTCCTGAAATATGCCCACCAGTGCCCACCGTTGGGAAAACGCCTCCAGGGAAGCCATGGACGTTGCTCTGATTATCAATGGTGTAGTAAAGTTCCCCCAATGTCGCCCCTGCTTCAACCCAAGCAGTTTCGTTTGGAATATCGACATTGATAGCTCGAAGGTTGAACATGTCGAGGAGGACGAAGGGGTGTTGGGAGACATAGGAGAGGCCTTCATAATCATGGCCGCCACTGCGGATTCTGATCTGAAGTCCAACACGTTTGGCACAAACAACGGTTCATTGAACATGGGATTGGTGTTTGGCTGCAACAATCACTAGGGGTTTTGGGGTTGTGGGGGTCTGAAATCTGAGGTTTCTGATGTAGAAATTGAGGACTGGTAAGAAGGAAGAATTGTTGGGAGTGAAAATGGCGTCAGAAATGGGGAATTGGGGCAGTGAATTGGTGCTTAGACATCGAAGAAAGGTCTGTGGAGCAGAAGCAGAGGATAGCCATGGAGAGAAAATGAAGATGACAGAGAAGAATGTAAAGATTGAAGAAGATGAAAACCCCGTCCCCATTTTT

mRNA sequence

TATGGGTGAATCTGAAAATGGTATTATGGGTGAATCTGAAAATGGTATTATGGGTGAATCTGAAAATGGTATTATGGGTGAATCTGAAAATGGTATGACAAAAGGTTTAAGGCGGCAATCATGAGCGGCCAAAATGCGTTACACAGCCTGACTCATTTCATTTCATTGAGCGGTGCAAATCCCTCTATTCCCCTTCAACATATAAACGCCACATTGCCCTTCAAATTCTCCAACAACTCACCACCATTTAACCAATCCATCAACCCCATTTTCGAGCCTTTGCTGCCTCTGCCTTTCGTTTTCCTTTCCAATCCACTCCTTCTGCATTCCGCTTTACTGCCCTCAGATCTGAACAAACGATGGTTGCACAGGAATTTACTGTAGATCTTAACAAACCACTTGTGTTCCAGGTTGGGCATCTTGGAGAAGCATATCAAGACTGGGTTCACCGCCCTATTGTTAGCAAGGAAAGGCCTCGATTCTTCAAAAGCGACTTCTGGGAGTTCTGTACACGCACAGCATGGTGGGTGGTTCCACTTATATGGCTGCCAGTGGTCTGTTGGTTCATTTCTATGTCTATGAACATGGGGCACACACTTGGTCAGATATCATTCATGGTCATCTTCGGCATTTATATTTGGACATTGATCGAATACATAGTGCATCGTTTTCTATTCCATATTGAAACCAGTAGTTATTGGGGGAATACCATGCATTATCTTATTCATGGTTGCCACCACAAGCACCCAATGGATAGTCTACGACTTGTTTTTCCTCCTACTGCCACAGCTGTTCTGATAGTTCCAATATGGAACTTGGTTAAACTGTTTTTCCCAACATCTATGGCGCCTGCTCTGTTTGGAGGTGGTCTTTTGGGCTATGTGATGTATGACGTTACCCATTACTACTTGCATCATGGCCAACCCACCGGAGAAATGCCGAAACATTTGAAGAAATATCACATGAATCATCACTTCCGCATCATGAATAAAGGCTTCGGTGTCACTTCATCACTTTGGGACAGAGCGTTTGGAACACTGCCTGCGTCAAAAAAATTGAGGACTGGTAAGAAGGAAGAATTGTTGGGAGTGAAAATGGCGTCAGAAATGGGGAATTGGGGCAGTGAATTGGTGCTTAGACATCGAAGAAAGGTCTGTGGAGCAGAAGCAGAGGATAGCCATGGAGAGAAAATGAAGATGACAGAGAAGAATGTAAAGATTGAAGAAGATGAAAACCCCGTCCCCATTTTT

Coding sequence (CDS)

ATGGTTGCACAGGAATTTACTGTAGATCTTAACAAACCACTTGTGTTCCAGGTTGGGCATCTTGGAGAAGCATATCAAGACTGGGTTCACCGCCCTATTGTTAGCAAGGAAAGGCCTCGATTCTTCAAAAGCGACTTCTGGGAGTTCTGTACACGCACAGCATGGTGGGTGGTTCCACTTATATGGCTGCCAGTGGTCTGTTGGTTCATTTCTATGTCTATGAACATGGGGCACACACTTGGTCAGATATCATTCATGGTCATCTTCGGCATTTATATTTGGACATTGATCGAATACATAGTGCATCGTTTTCTATTCCATATTGAAACCAGTAGTTATTGGGGGAATACCATGCATTATCTTATTCATGGTTGCCACCACAAGCACCCAATGGATAGTCTACGACTTGTTTTTCCTCCTACTGCCACAGCTGTTCTGATAGTTCCAATATGGAACTTGGTTAAACTGTTTTTCCCAACATCTATGGCGCCTGCTCTGTTTGGAGGTGGTCTTTTGGGCTATGTGATGTATGACGTTACCCATTACTACTTGCATCATGGCCAACCCACCGGAGAAATGCCGAAACATTTGAAGAAATATCACATGAATCATCACTTCCGCATCATGAATAAAGGCTTCGGTGTCACTTCATCACTTTGGGACAGAGCGTTTGGAACACTGCCTGCGTCAAAAAAATTGAGGACTGGTAAGAAGGAAGAATTGTTGGGAGTGAAAATGGCGTCAGAAATGGGGAATTGGGGCAGTGAATTGGTGCTTAGACATCGAAGAAAGGTCTGTGGAGCAGAAGCAGAGGATAGCCATGGAGAGAAAATGAAGATGACAGAGAAGAATGTAAAGATTGAAGAAGATGAAAACCCCGTCCCCATTTTT

Protein sequence

MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPLIWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHYLIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVTHYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASKKLRTGKKEELLGVKMASEMGNWGSELVLRHRRKVCGAEAEDSHGEKMKMTEKNVKIEEDENPVPIF
Homology
BLAST of Cp4.1LG19g05720 vs. ExPASy Swiss-Prot
Match: O48916 (Dihydroceramide fatty acyl 2-hydroxylase FAH1 OS=Arabidopsis thaliana OX=3702 GN=FAH1 PE=1 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 5.0e-101
Identity = 166/234 (70.94%), Postives = 192/234 (82.05%), Query Frame = 0

Query: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPL 60
           MVAQ FTVDL KPLVFQVGHLGE Y++WVH+PI +KE PRFF+SDFWEF T T WW VP+
Sbjct: 1   MVAQGFTVDLKKPLVFQVGHLGEDYEEWVHQPIATKEGPRFFQSDFWEFLTLTVWWAVPV 60

Query: 61  IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY 120
           IWLPVV W IS S++MG +L +I  +V+ GI+IWT  EY++HRF+FHI+T SYWGNT HY
Sbjct: 61  IWLPVVVWCISRSVSMGCSLPEIVPIVVMGIFIWTFFEYVLHRFVFHIKTKSYWGNTAHY 120

Query: 121 LIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT 180
           LIHGCHHKHPMD LRLVFPPTATA+L  P WN+ K     S APALFGGG+LGYVMYDVT
Sbjct: 121 LIHGCHHKHPMDHLRLVFPPTATAILCFPFWNIAKAISTPSTAPALFGGGMLGYVMYDVT 180

Query: 181 HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASKKLR 235
           HYYLHH QPT  + K+LKKYH+NHHFRI +KGFG+TSSLWD  FGTLP +K  R
Sbjct: 181 HYYLHHAQPTRPVTKNLKKYHLNHHFRIQDKGFGITSSLWDIVFGTLPTTKAPR 234

BLAST of Cp4.1LG19g05720 vs. ExPASy Swiss-Prot
Match: Q9SUC5 (Dihydroceramide fatty acyl 2-hydroxylase FAH2 OS=Arabidopsis thaliana OX=3702 GN=FAH2 PE=1 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 4.0e-98
Identity = 157/231 (67.97%), Postives = 186/231 (80.52%), Query Frame = 0

Query: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPL 60
           MVA+ +TVDLNKPLVFQVGHLGE YQ+W+H+PIV  E PRFF+SDFWEF TRT WW +P 
Sbjct: 1   MVAERYTVDLNKPLVFQVGHLGEEYQEWIHQPIVCVEGPRFFESDFWEFLTRTVWWAIPT 60

Query: 61  IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY 120
           IWLPVVC+ +S+S + G T  QI  +V FG+  WTL+EY +HRFLFHI+T SYW NT HY
Sbjct: 61  IWLPVVCYVLSISASKGLTFPQIGLIVAFGVLTWTLLEYTLHRFLFHIQTKSYWANTAHY 120

Query: 121 LIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT 180
           L+HGCHHKHP D LRLVFPPTATA+L+VP+W L+ L    + APA+ GG L GYVMYD+T
Sbjct: 121 LLHGCHHKHPQDGLRLVFPPTATAILLVPLWKLLHLLATPATAPAILGGILFGYVMYDIT 180

Query: 181 HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK 232
           HYYLHHGQP     KHLKKYH+NHHFRI +KG+G+TSSLWD+ FGTLP  K
Sbjct: 181 HYYLHHGQPKEPTFKHLKKYHLNHHFRIQDKGYGITSSLWDKVFGTLPGIK 231

BLAST of Cp4.1LG19g05720 vs. ExPASy Swiss-Prot
Match: Q2LAM0 (Fatty acid 2-hydroxylase OS=Rattus norvegicus OX=10116 GN=Fa2h PE=1 SV=2)

HSP 1 Score: 199.1 bits (505), Expect = 6.9e-50
Identity = 102/242 (42.15%), Postives = 151/242 (62.40%), Query Frame = 0

Query: 8   VDLNKPLVFQVGHLGEAYQDWVHRPIVSKERP-RFFKSDFWEFCTRTAWWVVPLIWLPVV 67
           VD  KPL++QVGHLGE Y +WVH+P+    RP R F SD  E  ++T W+ VP+IW+P+V
Sbjct: 124 VDWQKPLLWQVGHLGEKYDEWVHQPVA---RPIRLFHSDLIEAFSKTVWYSVPIIWVPLV 183

Query: 68  CW----------------FISMSMNMGHTLGQISFMVIF--GIYIWTLIEYIVHRFLFHI 127
            +                F S + +    + +  F+ +F  G+ IWTL+EY++HRFLFH+
Sbjct: 184 LYLSWSYYRTLTQDNIRLFASFTRDYSLVVPESVFIGLFVLGMLIWTLVEYLIHRFLFHM 243

Query: 128 E--TSSYWGNTMHYLIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPAL 187
           +  ++S++   +H+++HG HHK P D  RLVFPP   +V++   +  ++L  P ++A  L
Sbjct: 244 KPPSNSHYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASVVVAFFYVFLRLILPEAVAGIL 303

Query: 188 FGGGLLGYVMYDVTHYYLHHGQP-TGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFG 228
           F GGLLGYV+YD+THYYLH G P  G    ++K +H+ HHF     GFG+++ LWD  F 
Sbjct: 304 FAGGLLGYVLYDMTHYYLHFGSPHKGSYLYNMKAHHVKHHFEYQKSGFGISTKLWDYFFH 362

BLAST of Cp4.1LG19g05720 vs. ExPASy Swiss-Prot
Match: Q4R4P4 (Fatty acid 2-hydroxylase OS=Macaca fascicularis OX=9541 GN=FA2H PE=2 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 2.6e-49
Identity = 105/251 (41.83%), Postives = 151/251 (60.16%), Query Frame = 0

Query: 8   VDLNKPLVFQVGHLGEAYQDWVHRPIVSKERP-RFFKSDFWEFCTRTAWWVVPLIWLPVV 67
           VD  KPL++QVGHLGE Y +WVH+P+    RP R F SD  E  ++T W+ VP+IW+P+V
Sbjct: 124 VDWQKPLLWQVGHLGEKYDEWVHQPVT---RPIRLFHSDLIEGLSKTVWYSVPIIWVPLV 183

Query: 68  ---CWFISMSMNMGHTLGQISF---------------MVIFGIYIWTLIEYIVHRFLFHI 127
               W    +   G+     SF               + + GI++W+LIEY++HRFLFH+
Sbjct: 184 LYLSWSYYRTFAQGNVRLFTSFTTEYALAVPKSMFPGLFMLGIFLWSLIEYLIHRFLFHM 243

Query: 128 E--TSSYWGNTMHYLIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPAL 187
           +  + SY+   +H+++HG HHK P D  RLVFPP   +++I   +  ++L  P ++   +
Sbjct: 244 KPPSDSYYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASLVIGVFYLCLQLILPEAVGGTV 303

Query: 188 FGGGLLGYVMYDVTHYYLHHGQP-TGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFG 236
           F GGLLGYV+YD+THYYLH G P  G    +LK +H+ HHF     GFG+++ LWD  F 
Sbjct: 304 FAGGLLGYVLYDMTHYYLHFGSPHRGSYLYNLKAHHVKHHFAHQKSGFGISTKLWDYCFH 363

BLAST of Cp4.1LG19g05720 vs. ExPASy Swiss-Prot
Match: Q7L5A8 (Fatty acid 2-hydroxylase OS=Homo sapiens OX=9606 GN=FA2H PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.7e-48
Identity = 102/246 (41.46%), Postives = 146/246 (59.35%), Query Frame = 0

Query: 8   VDLNKPLVFQVGHLGEAYQDWVHRPIVSKERP-RFFKSDFWEFCTRTAWWVVPLIWLPVV 67
           VD  KPL++QVGHLGE Y +WVH+P+    RP R F SD  E  ++T W+ VP+IW+P+V
Sbjct: 124 VDWRKPLLWQVGHLGEKYDEWVHQPVT---RPIRLFHSDLIEGLSKTVWYSVPIIWVPLV 183

Query: 68  ---CWFISMSMNMGHTLGQISF---------------MVIFGIYIWTLIEYIVHRFLFHI 127
               W    +   G+     SF               + + G ++W+LIEY++HRFLFH+
Sbjct: 184 LYLSWSYYRTFAQGNVRLFTSFTTEYTVAVPKSMFPGLFMLGTFLWSLIEYLIHRFLFHM 243

Query: 128 E--TSSYWGNTMHYLIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPAL 187
           +  + SY+   +H+++HG HHK P D  RLVFPP   +++I   +  ++L  P ++   +
Sbjct: 244 KPPSDSYYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASLVIGVFYLCMQLILPEAVGGTV 303

Query: 188 FGGGLLGYVMYDVTHYYLHHGQP-TGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFG 232
           F GGLLGYV+YD+THYYLH G P  G     LK +H+ HHF     GFG+++ LWD  F 
Sbjct: 304 FAGGLLGYVLYDMTHYYLHFGSPHKGSYLYSLKAHHVKHHFAHQKSGFGISTKLWDYCFH 363

BLAST of Cp4.1LG19g05720 vs. NCBI nr
Match: XP_023518676.1 (dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita pepo subsp. pepo] >XP_023518677.1 dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 498 bits (1281), Expect = 6.61e-177
Identity = 231/231 (100.00%), Postives = 231/231 (100.00%), Query Frame = 0

Query: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPL 60
           MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPL
Sbjct: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPL 60

Query: 61  IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY 120
           IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY
Sbjct: 61  IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY 120

Query: 121 LIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT 180
           LIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT
Sbjct: 121 LIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT 180

Query: 181 HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK 231
           HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK
Sbjct: 181 HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK 231

BLAST of Cp4.1LG19g05720 vs. NCBI nr
Match: XP_022926333.1 (dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita moschata] >XP_022926334.1 dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita moschata])

HSP 1 Score: 496 bits (1278), Expect = 1.89e-176
Identity = 230/231 (99.57%), Postives = 231/231 (100.00%), Query Frame = 0

Query: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPL 60
           MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVP+
Sbjct: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPV 60

Query: 61  IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY 120
           IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY
Sbjct: 61  IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY 120

Query: 121 LIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT 180
           LIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT
Sbjct: 121 LIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT 180

Query: 181 HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK 231
           HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK
Sbjct: 181 HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK 231

BLAST of Cp4.1LG19g05720 vs. NCBI nr
Match: XP_023004067.1 (dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita maxima] >XP_023004068.1 dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita maxima])

HSP 1 Score: 496 bits (1276), Expect = 3.82e-176
Identity = 229/231 (99.13%), Postives = 231/231 (100.00%), Query Frame = 0

Query: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPL 60
           MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVP+
Sbjct: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPV 60

Query: 61  IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY 120
           IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY
Sbjct: 61  IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY 120

Query: 121 LIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT 180
           LIHGCHHKHPMDSLRLVFPPTATAVL+VPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT
Sbjct: 121 LIHGCHHKHPMDSLRLVFPPTATAVLLVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT 180

Query: 181 HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK 231
           HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK
Sbjct: 181 HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK 231

BLAST of Cp4.1LG19g05720 vs. NCBI nr
Match: KAG6594413.1 (Dihydroceramide fatty acyl 2-hydroxylase FAH1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 496 bits (1278), Expect = 1.06e-175
Identity = 230/231 (99.57%), Postives = 231/231 (100.00%), Query Frame = 0

Query: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPL 60
           MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVP+
Sbjct: 48  MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPV 107

Query: 61  IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY 120
           IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY
Sbjct: 108 IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY 167

Query: 121 LIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT 180
           LIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT
Sbjct: 168 LIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT 227

Query: 181 HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK 231
           HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK
Sbjct: 228 HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK 278

BLAST of Cp4.1LG19g05720 vs. NCBI nr
Match: XP_038882766.1 (dihydroceramide fatty acyl 2-hydroxylase FAH1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 463 bits (1191), Expect = 3.43e-163
Identity = 211/231 (91.34%), Postives = 223/231 (96.54%), Query Frame = 0

Query: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPL 60
           MVAQEFTVDLNKPLVFQVGHLGEAYQ+WVH+PI+SKERPRFFKSDFWEFCTRTAWW+VPL
Sbjct: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQEWVHQPIISKERPRFFKSDFWEFCTRTAWWMVPL 60

Query: 61  IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY 120
           IWLPVVCWF+SMS+NMGHTL QIS MVIFGI IWTLIEYIVHRFLFHIET SYWGNTMHY
Sbjct: 61  IWLPVVCWFVSMSVNMGHTLPQISLMVIFGISIWTLIEYIVHRFLFHIETQSYWGNTMHY 120

Query: 121 LIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT 180
           LIHGCHHKHPMDSLRLVFPPTATAVL+VP+WNLVKL FP SMAPALFGGGLLGYV+YDVT
Sbjct: 121 LIHGCHHKHPMDSLRLVFPPTATAVLLVPLWNLVKLSFPPSMAPALFGGGLLGYVIYDVT 180

Query: 181 HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK 231
           HYYLHHGQPTG++PK LKKYHMNHHFRI+NKGFGVTS LWDRAFGTLPASK
Sbjct: 181 HYYLHHGQPTGDVPKKLKKYHMNHHFRILNKGFGVTSPLWDRAFGTLPASK 231

BLAST of Cp4.1LG19g05720 vs. ExPASy TrEMBL
Match: A0A6J1EET4 (dihydroceramide fatty acyl 2-hydroxylase FAH1-like OS=Cucurbita moschata OX=3662 GN=LOC111433515 PE=3 SV=1)

HSP 1 Score: 496 bits (1278), Expect = 9.17e-177
Identity = 230/231 (99.57%), Postives = 231/231 (100.00%), Query Frame = 0

Query: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPL 60
           MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVP+
Sbjct: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPV 60

Query: 61  IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY 120
           IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY
Sbjct: 61  IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY 120

Query: 121 LIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT 180
           LIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT
Sbjct: 121 LIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT 180

Query: 181 HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK 231
           HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK
Sbjct: 181 HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK 231

BLAST of Cp4.1LG19g05720 vs. ExPASy TrEMBL
Match: A0A6J1KYF5 (dihydroceramide fatty acyl 2-hydroxylase FAH1-like OS=Cucurbita maxima OX=3661 GN=LOC111497491 PE=3 SV=1)

HSP 1 Score: 496 bits (1276), Expect = 1.85e-176
Identity = 229/231 (99.13%), Postives = 231/231 (100.00%), Query Frame = 0

Query: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPL 60
           MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVP+
Sbjct: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPV 60

Query: 61  IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY 120
           IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY
Sbjct: 61  IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY 120

Query: 121 LIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT 180
           LIHGCHHKHPMDSLRLVFPPTATAVL+VPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT
Sbjct: 121 LIHGCHHKHPMDSLRLVFPPTATAVLLVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT 180

Query: 181 HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK 231
           HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK
Sbjct: 181 HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK 231

BLAST of Cp4.1LG19g05720 vs. ExPASy TrEMBL
Match: A0A1S3AZM0 (dihydroceramide fatty acyl 2-hydroxylase FAH1-like OS=Cucumis melo OX=3656 GN=LOC103484299 PE=3 SV=1)

HSP 1 Score: 459 bits (1180), Expect = 7.87e-162
Identity = 207/231 (89.61%), Postives = 220/231 (95.24%), Query Frame = 0

Query: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPL 60
           MV+Q+FTVDLNKPLVFQVGHLGEAYQ+WVH+PI+SKERPRFFKSDFWEFCT TAWW VPL
Sbjct: 1   MVSQQFTVDLNKPLVFQVGHLGEAYQEWVHQPIISKERPRFFKSDFWEFCTCTAWWAVPL 60

Query: 61  IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY 120
           IWLPVVCWF+SMS+NMGHT  QIS MVIFGI IWTLIEYIVHRFLFHIET SYWGNTMHY
Sbjct: 61  IWLPVVCWFVSMSVNMGHTFSQISLMVIFGISIWTLIEYIVHRFLFHIETQSYWGNTMHY 120

Query: 121 LIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT 180
           LIHGCHHKHPMDSLRLVFPPTATAVL+VP+WNLVKLFFP+SMAPALFGGGLLGYV+YDVT
Sbjct: 121 LIHGCHHKHPMDSLRLVFPPTATAVLLVPLWNLVKLFFPSSMAPALFGGGLLGYVIYDVT 180

Query: 181 HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK 231
           HYYLHHGQPTG+MPK LKKYHMNHHFRI+NKGFGVTS LWDR FGTLP SK
Sbjct: 181 HYYLHHGQPTGDMPKKLKKYHMNHHFRILNKGFGVTSPLWDRVFGTLPGSK 231

BLAST of Cp4.1LG19g05720 vs. ExPASy TrEMBL
Match: A0A5A7U9P5 (Dihydroceramide fatty acyl 2-hydroxylase FAH1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G00710 PE=3 SV=1)

HSP 1 Score: 458 bits (1178), Expect = 1.59e-161
Identity = 207/231 (89.61%), Postives = 219/231 (94.81%), Query Frame = 0

Query: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPL 60
           MV+Q+FTVDLNKPLVFQVGHLGEAYQ+WVH+PI+SKERPRFFKSDFWEFCT TAWW VPL
Sbjct: 1   MVSQQFTVDLNKPLVFQVGHLGEAYQEWVHQPIISKERPRFFKSDFWEFCTCTAWWAVPL 60

Query: 61  IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY 120
           IWLPVVCWF+SMS+NMGHT  QIS MVIFGI IWTLIEYIVHRFLFHIET SYWGNTMHY
Sbjct: 61  IWLPVVCWFVSMSVNMGHTFSQISLMVIFGISIWTLIEYIVHRFLFHIETQSYWGNTMHY 120

Query: 121 LIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT 180
           LIHGCHHKHPMDSLRLVFPPTATAVL+VP+WNLVKLFFP SMAPALFGGGLLGYV+YDVT
Sbjct: 121 LIHGCHHKHPMDSLRLVFPPTATAVLLVPLWNLVKLFFPPSMAPALFGGGLLGYVIYDVT 180

Query: 181 HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK 231
           HYYLHHGQPTG+MPK LKKYHMNHHFRI+NKGFGVTS LWDR FGTLP SK
Sbjct: 181 HYYLHHGQPTGDMPKKLKKYHMNHHFRILNKGFGVTSPLWDRVFGTLPGSK 231

BLAST of Cp4.1LG19g05720 vs. ExPASy TrEMBL
Match: A0A0A0KIC4 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G525650 PE=3 SV=1)

HSP 1 Score: 452 bits (1164), Expect = 2.16e-159
Identity = 204/231 (88.31%), Postives = 217/231 (93.94%), Query Frame = 0

Query: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPL 60
           MV+Q+FTVDLNKPLVFQVGHLGEAYQ+WVH+PI+SKERPRFFKSDFWEFCT TAWW VPL
Sbjct: 1   MVSQQFTVDLNKPLVFQVGHLGEAYQEWVHQPIISKERPRFFKSDFWEFCTCTAWWAVPL 60

Query: 61  IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY 120
           IWLPVVCWF+SMS+NMGHT  QIS MVI GI IWTLIEYIVHRFLFHIET SYWGNTMHY
Sbjct: 61  IWLPVVCWFVSMSVNMGHTFSQISLMVILGISIWTLIEYIVHRFLFHIETQSYWGNTMHY 120

Query: 121 LIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT 180
           LIHGCHHKHPMDSLRLVFPPTATAVL+ P+WNLVKLFFP SMAPALFGGGLLGYV+YDVT
Sbjct: 121 LIHGCHHKHPMDSLRLVFPPTATAVLLFPLWNLVKLFFPPSMAPALFGGGLLGYVIYDVT 180

Query: 181 HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK 231
           HYYLHHGQPTG+MPK LKKYHMNHH+RI+NKGFGVTS LWDR FGTLP SK
Sbjct: 181 HYYLHHGQPTGDMPKKLKKYHMNHHYRILNKGFGVTSPLWDRVFGTLPGSK 231

BLAST of Cp4.1LG19g05720 vs. TAIR 10
Match: AT2G34770.1 (fatty acid hydroxylase 1 )

HSP 1 Score: 369.0 bits (946), Expect = 3.6e-102
Identity = 166/234 (70.94%), Postives = 192/234 (82.05%), Query Frame = 0

Query: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPL 60
           MVAQ FTVDL KPLVFQVGHLGE Y++WVH+PI +KE PRFF+SDFWEF T T WW VP+
Sbjct: 1   MVAQGFTVDLKKPLVFQVGHLGEDYEEWVHQPIATKEGPRFFQSDFWEFLTLTVWWAVPV 60

Query: 61  IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY 120
           IWLPVV W IS S++MG +L +I  +V+ GI+IWT  EY++HRF+FHI+T SYWGNT HY
Sbjct: 61  IWLPVVVWCISRSVSMGCSLPEIVPIVVMGIFIWTFFEYVLHRFVFHIKTKSYWGNTAHY 120

Query: 121 LIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT 180
           LIHGCHHKHPMD LRLVFPPTATA+L  P WN+ K     S APALFGGG+LGYVMYDVT
Sbjct: 121 LIHGCHHKHPMDHLRLVFPPTATAILCFPFWNIAKAISTPSTAPALFGGGMLGYVMYDVT 180

Query: 181 HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASKKLR 235
           HYYLHH QPT  + K+LKKYH+NHHFRI +KGFG+TSSLWD  FGTLP +K  R
Sbjct: 181 HYYLHHAQPTRPVTKNLKKYHLNHHFRIQDKGFGITSSLWDIVFGTLPTTKAPR 234

BLAST of Cp4.1LG19g05720 vs. TAIR 10
Match: AT4G20870.1 (fatty acid hydroxylase 2 )

HSP 1 Score: 359.4 bits (921), Expect = 2.8e-99
Identity = 157/231 (67.97%), Postives = 186/231 (80.52%), Query Frame = 0

Query: 1   MVAQEFTVDLNKPLVFQVGHLGEAYQDWVHRPIVSKERPRFFKSDFWEFCTRTAWWVVPL 60
           MVA+ +TVDLNKPLVFQVGHLGE YQ+W+H+PIV  E PRFF+SDFWEF TRT WW +P 
Sbjct: 1   MVAERYTVDLNKPLVFQVGHLGEEYQEWIHQPIVCVEGPRFFESDFWEFLTRTVWWAIPT 60

Query: 61  IWLPVVCWFISMSMNMGHTLGQISFMVIFGIYIWTLIEYIVHRFLFHIETSSYWGNTMHY 120
           IWLPVVC+ +S+S + G T  QI  +V FG+  WTL+EY +HRFLFHI+T SYW NT HY
Sbjct: 61  IWLPVVCYVLSISASKGLTFPQIGLIVAFGVLTWTLLEYTLHRFLFHIQTKSYWANTAHY 120

Query: 121 LIHGCHHKHPMDSLRLVFPPTATAVLIVPIWNLVKLFFPTSMAPALFGGGLLGYVMYDVT 180
           L+HGCHHKHP D LRLVFPPTATA+L+VP+W L+ L    + APA+ GG L GYVMYD+T
Sbjct: 121 LLHGCHHKHPQDGLRLVFPPTATAILLVPLWKLLHLLATPATAPAILGGILFGYVMYDIT 180

Query: 181 HYYLHHGQPTGEMPKHLKKYHMNHHFRIMNKGFGVTSSLWDRAFGTLPASK 232
           HYYLHHGQP     KHLKKYH+NHHFRI +KG+G+TSSLWD+ FGTLP  K
Sbjct: 181 HYYLHHGQPKEPTFKHLKKYHLNHHFRIQDKGYGITSSLWDKVFGTLPGIK 231

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O489165.0e-10170.94Dihydroceramide fatty acyl 2-hydroxylase FAH1 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9SUC54.0e-9867.97Dihydroceramide fatty acyl 2-hydroxylase FAH2 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q2LAM06.9e-5042.15Fatty acid 2-hydroxylase OS=Rattus norvegicus OX=10116 GN=Fa2h PE=1 SV=2[more]
Q4R4P42.6e-4941.83Fatty acid 2-hydroxylase OS=Macaca fascicularis OX=9541 GN=FA2H PE=2 SV=1[more]
Q7L5A81.7e-4841.46Fatty acid 2-hydroxylase OS=Homo sapiens OX=9606 GN=FA2H PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_023518676.16.61e-177100.00dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita pepo subsp. pepo] ... [more]
XP_022926333.11.89e-17699.57dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita moschata] >XP_0229... [more]
XP_023004067.13.82e-17699.13dihydroceramide fatty acyl 2-hydroxylase FAH1-like [Cucurbita maxima] >XP_023004... [more]
KAG6594413.11.06e-17599.57Dihydroceramide fatty acyl 2-hydroxylase FAH1, partial [Cucurbita argyrosperma s... [more]
XP_038882766.13.43e-16391.34dihydroceramide fatty acyl 2-hydroxylase FAH1-like isoform X2 [Benincasa hispida... [more]
Match NameE-valueIdentityDescription
A0A6J1EET49.17e-17799.57dihydroceramide fatty acyl 2-hydroxylase FAH1-like OS=Cucurbita moschata OX=3662... [more]
A0A6J1KYF51.85e-17699.13dihydroceramide fatty acyl 2-hydroxylase FAH1-like OS=Cucurbita maxima OX=3661 G... [more]
A0A1S3AZM07.87e-16289.61dihydroceramide fatty acyl 2-hydroxylase FAH1-like OS=Cucumis melo OX=3656 GN=LO... [more]
A0A5A7U9P51.59e-16189.61Dihydroceramide fatty acyl 2-hydroxylase FAH1-like OS=Cucumis melo var. makuwa O... [more]
A0A0A0KIC42.16e-15988.31Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C... [more]
Match NameE-valueIdentityDescription
AT2G34770.13.6e-10270.94fatty acid hydroxylase 1 [more]
AT4G20870.12.8e-9967.97fatty acid hydroxylase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006694Fatty acid hydroxylasePFAMPF04116FA_hydroxylasecoord: 88..226
e-value: 1.6E-15
score: 57.6
IPR014430Sterol desaturase Scs7PANTHERPTHR12863FATTY ACID HYDROXYLASEcoord: 4..233
NoneNo IPR availablePANTHERPTHR12863:SF1FATTY ACID 2-HYDROXYLASEcoord: 4..233

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG19g05720.1Cp4.1LG19g05720.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006631 fatty acid metabolic process
biological_process GO:0008610 lipid biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0080132 fatty acid alpha-hydroxylase activity
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016491 oxidoreductase activity