Cp4.1LG18g08160 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG18g08160
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionprotein NUCLEAR FUSION DEFECTIVE 4
LocationCp4.1LG18: 7561039 .. 7563716 (+)
RNA-Seq ExpressionCp4.1LG18g08160
SyntenyCp4.1LG18g08160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ACAAATGTCATACTCCGAGTCAGACTTGCCTCCAAGAATATGGGATGGGATCGATTTCACCAATTTTCCGACAAAAGTCAAAATAAAAATTAAAAAAAGACCAAACATGAGCCACACAATCAAATCAATTCCTGACCGAACACGTATACCGCCTACAACACTGAAATGCTTCTACTTTACAACGAAGAACGAGAGCCAGAAGAAGAACCCACCGCCGGAGATGGAAGCATCGGGGTTTATGGCCAGTAAATGGATTGCAACGGCGTTTGCGATTTGGATCCAATGCAGCTGCGGTGCTGCCACCTTCAGCATCTACTCGTCGGCTCTCAAGTCCTCACAGGGCTACGACCAATCCACCCTTGGCACCGTTTCCGTTTTCAAGGACATCGGCACCAACGCCGGCATCATTTCTGGCTTTCTTTGCTCTGCTGTCACTTCCACCTTCCCCCGCCGACCCTTTGTTGGGCCATGGATGGTGTACGCGGCGGGAGCGGTTCAGTGCTTCCTCGGATATATTTTCATGTGGGCCGCTGTCTCCGGACTGATCGATCGGCCGTCGGTTCCAGTGATGTGCTTCTTCATGTTGCTTGCTTCACATGCGCAAACGTTTTTCAGTACGGCGAATGTGGTAACTGGCGTGCACAATTTCAAGAACTATAGCGGCACGATTGTGGGCATCATGAAGGTACCAACCCAAACCCTTAGTTCTGAATCCTTCTTACCCTTGTCGTATTATTCAATACCGTAGAGTATCTACTTTCCAATTTATGCTCATAATTACCTGTAACCATTGGTTTTGTACTGTTTCCCCTGTTCTCCTCCCCCAACTAACATCTCAAGCTCACATGAGATCTCACCATTCACCTATTCAGGATAGAGCGTCCTCGCTGACACACAGCTTAATTTAGTTTCTTCGCTTGTACATCCCCTGTTATCTAGCTCTAATACCATTTGTAACATAATGTTTTGTTCTCCTCTTTAACTAACATGCGATCTCACAATTTAGCCTCACTGGTGCTCAACCTCATGTCTACCCCCTTCGAGATTTAGCCTCCTCACATGAGATTTCACAATTTACCCCTCCGGAACCAGCGTTCTCACTGGCACACAGTCTCATGTCTACCCTCTTCGGGCTTTGGCCTCCTCACATGGGATTTCACAATCCATCCCTTCGGGAACCAGCGTACTCATTGGCACACAGTCTCATGTCTACCCCTTCGGGATTTAGCCTCCTCACATGGGGATCTCACAATCCACCCCTTCGGGACCCAACGTCCTCACTGGCACATAGCCTCATGTCTACCCCTTCGGAATTTAACCTCCTCACATGTGATCTCACAATCCACCCTTTGGAACTAGCGTCCTCACTAGCACACAGCTTTATATCTACTCCTTCGAGATTTAGCCTCATCGCTGGCACATCGCCCGGTGTTAGACTCTGATACCATTTGTAACTGTTAAAGGAACGGTGTTAGGCTCTAATACTATTTGTAACAGTTGAAGGAACTCTTTAAATGTGTGCGTTTTTTGTTGTTGTATAGGGATATCTTGGTCTAAGTGGAGCACTGGTAATCCAAGTTTATAACACGATTTGCAATGGAGAACCAAGCAATTTTCTCCTCTTGCTGGCGGTTTTGCCCACACTTCTTTCCATCATGTTCATGTGGTTTATCAGAATTGACAGAACAGAGTCTAAAAACGAGAGGAAGCATTTGGACTCATTGTCAGCGCATGCCGTGATAGTTGCTTTTTACCTAATGGCTGTCATCATTTTGGACAACACTTTCTCCCTTCCATCATCGACTCGTTCCTTCACATTCTCCATACTCCTAATTCTTCTTTCTGCTCCTCTTGGAATCGCAATTAATGCTCGGAGGGAGGACTTAAAAGGGACCGAAAAAACCCATGTTCTTAACAAATCTAAGTCAGTGGATACAGAGGATCTTGTTGAGTATCACGAGTTGCCCAGGGACGATGGCCAGATAATGGCTGTTGCAAGCTCTAGTACCCCACAAGCCATGAATGTGTTGGAAGCTTTATGCACTATAAACTTCTGGCTGTTGTTTACAGCCATGGTGTGTGGAATGGGTTCTGGATTAGCCACAATAAACAACATGACACAGCTTGGCCAGTCTCTTGGCTACACAGTAACAGAGACGAAGACATTTGTTTCCTTATGGAGTATATGGAACTTTCTTGGACGTTTTGGAGCTGGTTACGCCTCTGATTATCTTCTCCACTCATACGGCTGTGCCAGGCCATTGTTAATGGCAATCACTCTACTCATAATGAGCGGTGGGCACCTCGTGATCGCCTCAAGTTTCTCTGGAAATCTTTACGTGGGTTCAACTCTTGTTGGCATATGCTACGGCTCACAGTGGTCGCTCATGCCCTCAATTACTTCGGACATATTTGGGATAGAGCATATGGGGACTATTTTCAACGCCATAGCCACAGCCAGCCCTCTGGGGTCGTACGTTTTTTCTGTTAAAGTGATCGGTTACATCTACGACAGGGAAGCTGCCGGAGAACATGGGTCCTGCTCTGGCACACACTGTTTTGCGGTGTCTTTTCTAGTCATGGCCGTCGTGGCTTTCGTGGGATTTCTGGTAGCTGCTGCTCTCTTCTTTCGAACCAGGAGATTTTACGCTTTGCAAAGTAGAGCCGAACGTCTCAGT

mRNA sequence

ACAAATGTCATACTCCGAGTCAGACTTGCCTCCAAGAATATGGGATGGGATCGATTTCACCAATTTTCCGACAAAAGTCAAAATAAAAATTAAAAAAAGACCAAACATGAGCCACACAATCAAATCAATTCCTGACCGAACACGTATACCGCCTACAACACTGAAATGCTTCTACTTTACAACGAAGAACGAGAGCCAGAAGAAGAACCCACCGCCGGAGATGGAAGCATCGGGGTTTATGGCCAGTAAATGGATTGCAACGGCGTTTGCGATTTGGATCCAATGCAGCTGCGGTGCTGCCACCTTCAGCATCTACTCGTCGGCTCTCAAGTCCTCACAGGGCTACGACCAATCCACCCTTGGCACCGTTTCCGTTTTCAAGGACATCGGCACCAACGCCGGCATCATTTCTGGCTTTCTTTGCTCTGCTGTCACTTCCACCTTCCCCCGCCGACCCTTTGTTGGGCCATGGATGGTGTACGCGGCGGGAGCGGTTCAGTGCTTCCTCGGATATATTTTCATGTGGGCCGCTGTCTCCGGACTGATCGATCGGCCGTCGGTTCCAGTGATGTGCTTCTTCATGTTGCTTGCTTCACATGCGCAAACGTTTTTCAGTACGGCGAATGTGGTAACTGGCGTGCACAATTTCAAGAACTATAGCGGCACGATTGTGGGCATCATGAAGAACCAAGCAATTTTCTCCTCTTGCTGGCGAATTGACAGAACAGAGTCTAAAAACGAGAGGAAGCATTTGGACTCATTGTCAGCGCATGCCGTGATAGTTGCTTTTTACCTAATGGCTGTCATCATTTTGGACAACACTTTCTCCCTTCCATCATCGACTCGTTCCTTCACATTCTCCATACTCCTAATTCTTCTTTCTGCTCCTCTTGGAATCGCAATTAATGCTCGGAGGGAGGACTTAAAAGGGACCGAAAAAACCCATGTTCTTAACAAATCTAAGTCAGTGGATACAGAGGATCTTGTTGAGTATCACGAGTTGCCCAGGGACGATGGCCAGATAATGGCTGTTGCAAGCTCTAGTACCCCACAAGCCATGAATGTGTTGGAAGCTTTATGCACTATAAACTTCTGGCTGTTGTTTACAGCCATGGTGTGTGGAATGGGTTCTGGATTAGCCACAATAAACAACATGACACAGCTTGGCCAGTCTCTTGGCTACACAGTAACAGAGACGAAGACATTTGTTTCCTTATGGAGTATATGGAACTTTCTTGGACGTTTTGGAGCTGGTTACGCCTCTGATTATCTTCTCCACTCATACGGCTGTGCCAGGCCATTGTTAATGGCAATCACTCTACTCATAATGAGCGGTGGGCACCTCGTGATCGCCTCAAGTTTCTCTGGAAATCTTTACGTGGGTTCAACTCTTGTTGGCATATGCTACGGCTCACAGTGGTCGCTCATGCCCTCAATTACTTCGGACATATTTGGGATAGAGCATATGGGGACTATTTTCAACGCCATAGCCACAGCCAGCCCTCTGGGGTCGTACGTTTTTTCTGTTAAAGTGATCGGTTACATCTACGACAGGGAAGCTGCCGGAGAACATGGGTCCTGCTCTGGCACACACTGTTTTGCGGTGTCTTTTCTAGTCATGGCCGTCGTGGCTTTCGTGGGATTTCTGGTAGCTGCTGCTCTCTTCTTTCGAACCAGGAGATTTTACGCTTTGCAAAGTAGAGCCGAACGTCTCAGT

Coding sequence (CDS)

ATGTCATACTCCGAGTCAGACTTGCCTCCAAGAATATGGGATGGGATCGATTTCACCAATTTTCCGACAAAAGTCAAAATAAAAATTAAAAAAAGACCAAACATGAGCCACACAATCAAATCAATTCCTGACCGAACACGTATACCGCCTACAACACTGAAATGCTTCTACTTTACAACGAAGAACGAGAGCCAGAAGAAGAACCCACCGCCGGAGATGGAAGCATCGGGGTTTATGGCCAGTAAATGGATTGCAACGGCGTTTGCGATTTGGATCCAATGCAGCTGCGGTGCTGCCACCTTCAGCATCTACTCGTCGGCTCTCAAGTCCTCACAGGGCTACGACCAATCCACCCTTGGCACCGTTTCCGTTTTCAAGGACATCGGCACCAACGCCGGCATCATTTCTGGCTTTCTTTGCTCTGCTGTCACTTCCACCTTCCCCCGCCGACCCTTTGTTGGGCCATGGATGGTGTACGCGGCGGGAGCGGTTCAGTGCTTCCTCGGATATATTTTCATGTGGGCCGCTGTCTCCGGACTGATCGATCGGCCGTCGGTTCCAGTGATGTGCTTCTTCATGTTGCTTGCTTCACATGCGCAAACGTTTTTCAGTACGGCGAATGTGGTAACTGGCGTGCACAATTTCAAGAACTATAGCGGCACGATTGTGGGCATCATGAAGAACCAAGCAATTTTCTCCTCTTGCTGGCGAATTGACAGAACAGAGTCTAAAAACGAGAGGAAGCATTTGGACTCATTGTCAGCGCATGCCGTGATAGTTGCTTTTTACCTAATGGCTGTCATCATTTTGGACAACACTTTCTCCCTTCCATCATCGACTCGTTCCTTCACATTCTCCATACTCCTAATTCTTCTTTCTGCTCCTCTTGGAATCGCAATTAATGCTCGGAGGGAGGACTTAAAAGGGACCGAAAAAACCCATGTTCTTAACAAATCTAAGTCAGTGGATACAGAGGATCTTGTTGAGTATCACGAGTTGCCCAGGGACGATGGCCAGATAATGGCTGTTGCAAGCTCTAGTACCCCACAAGCCATGAATGTGTTGGAAGCTTTATGCACTATAAACTTCTGGCTGTTGTTTACAGCCATGGTGTGTGGAATGGGTTCTGGATTAGCCACAATAAACAACATGACACAGCTTGGCCAGTCTCTTGGCTACACAGTAACAGAGACGAAGACATTTGTTTCCTTATGGAGTATATGGAACTTTCTTGGACGTTTTGGAGCTGGTTACGCCTCTGATTATCTTCTCCACTCATACGGCTGTGCCAGGCCATTGTTAATGGCAATCACTCTACTCATAATGAGCGGTGGGCACCTCGTGATCGCCTCAAGTTTCTCTGGAAATCTTTACGTGGGTTCAACTCTTGTTGGCATATGCTACGGCTCACAGTGGTCGCTCATGCCCTCAATTACTTCGGACATATTTGGGATAGAGCATATGGGGACTATTTTCAACGCCATAGCCACAGCCAGCCCTCTGGGGTCGTACGTTTTTTCTGTTAAAGTGATCGGTTACATCTACGACAGGGAAGCTGCCGGAGAACATGGGTCCTGCTCTGGCACACACTGTTTTGCGGTGTCTTTTCTAGTCATGGCCGTCGTGGCTTTCGTGGGATTTCTGGTAGCTGCTGCTCTCTTCTTTCGAACCAGGAGATTTTACGCTTTGCAAAGTAGAGCCGAACGTCTCAGT

Protein sequence

MSYSESDLPPRIWDGIDFTNFPTKVKIKIKKRPNMSHTIKSIPDRTRIPPTTLKCFYFTTKNESQKKNPPPEMEASGFMASKWIATAFAIWIQCSCGAATFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNAGIISGFLCSAVTSTFPRRPFVGPWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCFFMLLASHAQTFFSTANVVTGVHNFKNYSGTIVGIMKNQAIFSSCWRIDRTESKNERKHLDSLSAHAVIVAFYLMAVIILDNTFSLPSSTRSFTFSILLILLSAPLGIAINARREDLKGTEKTHVLNKSKSVDTEDLVEYHELPRDDGQIMAVASSSTPQAMNVLEALCTINFWLLFTAMVCGMGSGLATINNMTQLGQSLGYTVTETKTFVSLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLLIMSGGHLVIASSFSGNLYVGSTLVGICYGSQWSLMPSITSDIFGIEHMGTIFNAIATASPLGSYVFSVKVIGYIYDREAAGEHGSCSGTHCFAVSFLVMAVVAFVGFLVAAALFFRTRRFYALQSRAERLS
Homology
BLAST of Cp4.1LG18g08160 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 1.5e-21
Identity = 140/548 (25.55%), Postives = 232/548 (42.34%), Query Frame = 0

Query: 82  KWIATAFAIWIQCSCGA-ATFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNAGIISGFLC 141
           KW     AIWIQ S G    FS YSS LKS  G  Q  L  ++V  D+G   G  SG   
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGI-- 102

Query: 142 SAVTSTFPRRPFVGPWMVYAAGAVQCFLGYIFMWAAVSGLIDRP-SVPVMCFFMLLASHA 201
                 FP        +V  A A   F+GY   W  ++ +I  P S+  +C   LLA  +
Sbjct: 103 --ALGYFPLS------VVLFAAAAMGFVGYGVQWLVITNIITLPYSLVFLC--CLLAGLS 162

Query: 202 QTFFSTANVVTGVHNFKNYSGTIVGIMK-----NQAIFSSCWR----------------- 261
             +F+TA  +  + +F N     + +       + A++S  +                  
Sbjct: 163 ICWFNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLV 222

Query: 262 -----------------IDRTESKNERKH----LDSLSAHAVIVAFYLMAVIILDNTFSL 321
                            +D T   + R+H       L+  AVI +F+L+      ++ S 
Sbjct: 223 PLVVSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLL-----SSSST 282

Query: 322 PSSTRSFTFSILLIL--LSAPLGI--------AINAR-REDLKGTEKTHV---LNKSKSV 381
            S+  +F  +++L++  L APL +         INAR   +  G    ++    N+  SV
Sbjct: 283 SSARLNFIGAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSV 342

Query: 382 DTEDLVEYHELPRDDGQIMAVASSSTPQAMNVLEALCTINFWLLFTAMVCGMGSGLATIN 441
            ++   E+    ++   +      S    ++ LE      FWL + A  CG   GL   N
Sbjct: 343 SSKTGYEHMGTAKEGNTVRLGDEHSFRLLISRLE------FWLYYIAYFCGGTIGLVYSN 402

Query: 442 NMTQLGQSLGYTVTETKTFVSLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLL-- 501
           N+ Q+ QSLG   T   T V+++S ++F GR     A D++   +   R    AI LL  
Sbjct: 403 NLGQIAQSLGQNST---TLVTIYSSFSFFGRL-LSAAPDFMHKRFRLTRTGWFAIALLPT 462

Query: 502 IMSGGHLVIASSFSGNLYVGSTLVGICYGSQWSLMPSITSDIFGIEHMGTIFNAIATASP 561
            ++   L ++SS    L   + L+G+  G  ++   SITSD+FG   +G   N + T  P
Sbjct: 463 PIAFFLLAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIP 522

BLAST of Cp4.1LG18g08160 vs. ExPASy Swiss-Prot
Match: Q6CGU8 (Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.1e-08
Identity = 49/167 (29.34%), Postives = 93/167 (55.69%), Query Frame = 0

Query: 400 TFVSLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLLIMSGGHLVIASS----FSG 459
           T VSL++ ++ + R   G++S+ +      +RP+L+++  L+ +  HL++ S     F  
Sbjct: 314 THVSLFATFSTVSRLVVGFSSEAM--ESHVSRPVLLSVIALVAACIHLMVPSGIFTVFDN 373

Query: 460 NLY--VGSTLVGICYGSQWSLMPSITSDIFGIEHMGTIFNAIATASPLGSYVFSVKVIGY 519
             Y  V + + G  YGS ++L+P+I + ++GI ++GTI+ +   A  +GS  + + +   
Sbjct: 374 AKYFSVVTIVNGFSYGSSFTLVPTIVTKVWGIANLGTIWGSFILALAVGSLGYGL-LFAK 433

Query: 520 IYDREAAGEHGS----CSGTHCFAVSFLVMAV-VAFVGFLVAAALFF 556
           +YD  +    GS    CSG HC+ ++F++    +AF     AAA+FF
Sbjct: 434 VYDAASEVGVGSMSQVCSGVHCYGLTFVITGTGLAF----AAAAVFF 473

BLAST of Cp4.1LG18g08160 vs. ExPASy Swiss-Prot
Match: Q6BN11 (Probable transporter MCH1 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=MCH1 PE=3 SV=2)

HSP 1 Score: 47.4 bits (111), Expect = 6.4e-04
Identity = 46/168 (27.38%), Postives = 79/168 (47.02%), Query Frame = 0

Query: 364 WLLFTAMVCGMGSGLATINNMTQLGQSLGYTVTETKTF--VSLWSIWNFLGRFGAGYASD 423
           WLL  +++  +G   +  NN   LG  +  + +E+     VS+ +  + + R   G  SD
Sbjct: 290 WLLLASLILNIGPMESFQNN---LGSIIINSNSESNLSDQVSIMAASSTVTRLAMGGLSD 349

Query: 424 YLLHS---YGCARPLLMAITLLIMSGGHLVIASSFSGNLYVGSTLVGICYGSQWSLMPSI 483
           YL  S   +   R  L+ I L I   G  ++    S    + S L G  YG  +++ P+I
Sbjct: 350 YLSSSKRKFPICRVNLLIINLAIGIVGQFMVTR--STRFSIVSILNGSSYGGLFTIYPTI 409

Query: 484 TSDIFGIEHMGTIFNAIATASPLGSYVFSVKVIGYIYDREAAGEHGSC 527
            + I+GI+ MG+ + +   A  +GS  FS+   G   D +   ++ SC
Sbjct: 410 VASIWGIDMMGSTWGSFMIAPAIGSIGFSI-FYGNEVDNKCGVDYSSC 451

BLAST of Cp4.1LG18g08160 vs. ExPASy Swiss-Prot
Match: Q4WVT3 (Probable transporter mch1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mch1 PE=3 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 8.4e-04
Identity = 36/121 (29.75%), Postives = 62/121 (51.24%), Query Frame = 0

Query: 440 LIMSGGHLVIAS----SFSGNLYVGSTLVGICYGSQWSLMPSITSDIFGIEHMGTIFNAI 499
           L++S G+L+++S       G  +V + L+G+ YGS +SL+P I S ++G+E+ GT +  +
Sbjct: 488 LLLSLGYLLLSSPLPLQHPGLSHVTTALIGLGYGSAFSLVPIIISVVWGVENFGTNWGIV 547

Query: 500 ATASPLGSYVFSVKVIGYIYDREAAGE---HGSCSGTHCF---AVSFLVMAVVAFVGFLV 551
           A     G+ ++ V       D    G     G C G  C+   AV   +   VA V +++
Sbjct: 548 AMVPAAGAAMWGVIYSRGYQDATDGGNGSPDGQCHGWRCYGFWAVGCTLSVWVAVVAWIL 607

BLAST of Cp4.1LG18g08160 vs. NCBI nr
Match: KAG6589488.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023173.1 Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 999 bits (2583), Expect = 0.0
Identity = 521/572 (91.08%), Postives = 527/572 (92.13%), Query Frame = 0

Query: 35  MSHTIKSIPDRTRIPPTTLKCFYFTTKNESQKKNPPPEMEASGFMASKWIATAFAIWIQC 94
           MSHTIKSIPDRTRIP TTLKCFYFTTKNESQKKNPPPEMEASGFMASKWIATAFAIWIQC
Sbjct: 1   MSHTIKSIPDRTRIPSTTLKCFYFTTKNESQKKNPPPEMEASGFMASKWIATAFAIWIQC 60

Query: 95  SCGAATFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNAGIISGFLCSAVTSTFPRRPFVG 154
           SCGAATFSIYSS LKSSQGYDQSTLGTVSVFKDIGTNAGIISGFLCSAVTS FPRRPFVG
Sbjct: 61  SCGAATFSIYSSVLKSSQGYDQSTLGTVSVFKDIGTNAGIISGFLCSAVTSPFPRRPFVG 120

Query: 155 PWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCFFMLLASHAQTFFSTANVVTGVHN 214
           PWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCFFMLLASHAQTFFSTANVVTGVHN
Sbjct: 121 PWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCFFMLLASHAQTFFSTANVVTGVHN 180

Query: 215 FKNYSGTIVGIMKN----------QAIFSSC-----------------------W--RID 274
           FK+YSGTIVGIMK           Q   + C                       W  RID
Sbjct: 181 FKHYSGTIVGIMKGYLGLSGALVIQVYNTICNGEPSNFLLLLAVLPTLLSIMFMWFIRID 240

Query: 275 RTESKNERKHLDSLSAHAVIVAFYLMAVIILDNTFSLPSSTRSFTFSILLILLSAPLGIA 334
           RTES+NERKHLDSLSAHAVIVAFYLMAVIILDNTFSLPSSTRSFTFSILLILLSAPLGIA
Sbjct: 241 RTESRNERKHLDSLSAHAVIVAFYLMAVIILDNTFSLPSSTRSFTFSILLILLSAPLGIA 300

Query: 335 INARREDLKGTEKTHVLNKSKSVDTEDLVEYHELPRDDGQIMAVASSSTPQAMNVLEALC 394
           INARREDLK TEKTHVLNKSKSVDTEDLVEYHELPRD+ QIMAVASSSTPQAMNVLEALC
Sbjct: 301 INARREDLKVTEKTHVLNKSKSVDTEDLVEYHELPRDERQIMAVASSSTPQAMNVLEALC 360

Query: 395 TINFWLLFTAMVCGMGSGLATINNMTQLGQSLGYTVTETKTFVSLWSIWNFLGRFGAGYA 454
           TINFWLLFTAMVCGMGSGLATINNMTQLGQSLGYTVTETKTFVSLWSIWNFLGRFGAGYA
Sbjct: 361 TINFWLLFTAMVCGMGSGLATINNMTQLGQSLGYTVTETKTFVSLWSIWNFLGRFGAGYA 420

Query: 455 SDYLLHSYGCARPLLMAITLLIMSGGHLVIASSFSGNLYVGSTLVGICYGSQWSLMPSIT 514
           SDYLLHSYGCARPLLMAITLLIMSGGHLVIASSFSGNLYVGSTLVGICYGSQWSLMPSIT
Sbjct: 421 SDYLLHSYGCARPLLMAITLLIMSGGHLVIASSFSGNLYVGSTLVGICYGSQWSLMPSIT 480

Query: 515 SDIFGIEHMGTIFNAIATASPLGSYVFSVKVIGYIYDREAAGEHGSCSGTHCFAVSFLVM 571
           SDIFG+EHMGTIFNAIATASPLGSYVFSVKVIGYIYD+EAAGEHGSCSGTHCFAVSFLVM
Sbjct: 481 SDIFGLEHMGTIFNAIATASPLGSYVFSVKVIGYIYDKEAAGEHGSCSGTHCFAVSFLVM 540

BLAST of Cp4.1LG18g08160 vs. NCBI nr
Match: XP_023515641.1 (uncharacterized protein LOC111779750 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 939 bits (2428), Expect = 0.0
Identity = 493/534 (92.32%), Postives = 494/534 (92.51%), Query Frame = 0

Query: 73  MEASGFMASKWIATAFAIWIQCSCGAATFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNA 132
           MEASGFMASKWIATAFAIWIQCSCGAATFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNA
Sbjct: 1   MEASGFMASKWIATAFAIWIQCSCGAATFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNA 60

Query: 133 GIISGFLCSAVTSTFPRRPFVGPWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCFF 192
           GIISGFLCSAVTSTFPRRPFVGPWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCFF
Sbjct: 61  GIISGFLCSAVTSTFPRRPFVGPWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCFF 120

Query: 193 MLLASHAQTFFSTANVVTGVHNFKNYSGTIVGIMKN----------QAIFSSC------- 252
           MLLASHAQTFFSTANVVTGVHNFKNYSGTIVGIMK           Q   + C       
Sbjct: 121 MLLASHAQTFFSTANVVTGVHNFKNYSGTIVGIMKGYLGLSGALVIQVYNTICNGEPSNF 180

Query: 253 ----------------W--RIDRTESKNERKHLDSLSAHAVIVAFYLMAVIILDNTFSLP 312
                           W  RIDRTESKNERKHLDSLSAHAVIVAFYLMAVIILDNTFSLP
Sbjct: 181 LLLLAVLPTLLSIMFMWFIRIDRTESKNERKHLDSLSAHAVIVAFYLMAVIILDNTFSLP 240

Query: 313 SSTRSFTFSILLILLSAPLGIAINARREDLKGTEKTHVLNKSKSVDTEDLVEYHELPRDD 372
           SSTRSFTFSILLILLSAPLGIAINARREDLKGTEKTHVLNKSKSVDTEDLVEYHELPRDD
Sbjct: 241 SSTRSFTFSILLILLSAPLGIAINARREDLKGTEKTHVLNKSKSVDTEDLVEYHELPRDD 300

Query: 373 GQIMAVASSSTPQAMNVLEALCTINFWLLFTAMVCGMGSGLATINNMTQLGQSLGYTVTE 432
           GQIMAVASSSTPQAMNVLEALCTINFWLLFTAMVCGMGSGLATINNMTQLGQSLGYTVTE
Sbjct: 301 GQIMAVASSSTPQAMNVLEALCTINFWLLFTAMVCGMGSGLATINNMTQLGQSLGYTVTE 360

Query: 433 TKTFVSLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLLIMSGGHLVIASSFSGNL 492
           TKTFVSLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLLIMSGGHLVIASSFSGNL
Sbjct: 361 TKTFVSLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLLIMSGGHLVIASSFSGNL 420

Query: 493 YVGSTLVGICYGSQWSLMPSITSDIFGIEHMGTIFNAIATASPLGSYVFSVKVIGYIYDR 552
           YVGSTLVGICYGSQWSLMPSITSDIFGIEHMGTIFNAIATASPLGSYVFSVKVIGYIYDR
Sbjct: 421 YVGSTLVGICYGSQWSLMPSITSDIFGIEHMGTIFNAIATASPLGSYVFSVKVIGYIYDR 480

Query: 553 EAAGEHGSCSGTHCFAVSFLVMAVVAFVGFLVAAALFFRTRRFYALQSRAERLS 571
           EAAGEHGSCSGTHCFAVSFLVMAVVAFVGFLVAAALFFRTRRFYALQSRAERLS
Sbjct: 481 EAAGEHGSCSGTHCFAVSFLVMAVVAFVGFLVAAALFFRTRRFYALQSRAERLS 534

BLAST of Cp4.1LG18g08160 vs. NCBI nr
Match: XP_022921338.1 (uncharacterized protein LOC111429640 [Cucurbita moschata])

HSP 1 Score: 925 bits (2391), Expect = 0.0
Identity = 486/534 (91.01%), Postives = 491/534 (91.95%), Query Frame = 0

Query: 73  MEASGFMASKWIATAFAIWIQCSCGAATFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNA 132
           MEASGFMASKWIATAFAIWIQCSCGAATFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNA
Sbjct: 1   MEASGFMASKWIATAFAIWIQCSCGAATFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNA 60

Query: 133 GIISGFLCSAVTSTFPRRPFVGPWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCFF 192
           GIISGFLCSAVTS FPRRPFVGPWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCFF
Sbjct: 61  GIISGFLCSAVTSPFPRRPFVGPWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCFF 120

Query: 193 MLLASHAQTFFSTANVVTGVHNFKNYSGTIVGIMKN----------QAIFSSC------- 252
           MLLASHAQTFFSTANVVTGVHNFK+YSGTIVGIMK           Q   + C       
Sbjct: 121 MLLASHAQTFFSTANVVTGVHNFKHYSGTIVGIMKGYLGLSGALVIQVYNTICNGEPSNF 180

Query: 253 ----------------W--RIDRTESKNERKHLDSLSAHAVIVAFYLMAVIILDNTFSLP 312
                           W  RIDRTES+NERKHLDSLSAHAVIVAFYLMAVIILDNTFSLP
Sbjct: 181 LLLLAVLPTLLSIMFMWFIRIDRTESRNERKHLDSLSAHAVIVAFYLMAVIILDNTFSLP 240

Query: 313 SSTRSFTFSILLILLSAPLGIAINARREDLKGTEKTHVLNKSKSVDTEDLVEYHELPRDD 372
           SSTRSFTFSILLILLSAPLGIAINARREDLK TEKTHVLNKSKSVDTEDLVEYHELPRD+
Sbjct: 241 SSTRSFTFSILLILLSAPLGIAINARREDLKVTEKTHVLNKSKSVDTEDLVEYHELPRDE 300

Query: 373 GQIMAVASSSTPQAMNVLEALCTINFWLLFTAMVCGMGSGLATINNMTQLGQSLGYTVTE 432
            QIMAVASSSTPQAMNVLEALCTINFWLLFTAMVCGMGSGLATINNMTQLGQSLGYTVTE
Sbjct: 301 RQIMAVASSSTPQAMNVLEALCTINFWLLFTAMVCGMGSGLATINNMTQLGQSLGYTVTE 360

Query: 433 TKTFVSLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLLIMSGGHLVIASSFSGNL 492
           TKTFVSLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLLIMSGGHLVIASSFSGNL
Sbjct: 361 TKTFVSLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLLIMSGGHLVIASSFSGNL 420

Query: 493 YVGSTLVGICYGSQWSLMPSITSDIFGIEHMGTIFNAIATASPLGSYVFSVKVIGYIYDR 552
           YVGSTLVGICYGSQWSLMPSITSDIFG+EHMGTIFNAIATASPLGSYVFSVKVIGYIYDR
Sbjct: 421 YVGSTLVGICYGSQWSLMPSITSDIFGLEHMGTIFNAIATASPLGSYVFSVKVIGYIYDR 480

Query: 553 EAAGEHGSCSGTHCFAVSFLVMAVVAFVGFLVAAALFFRTRRFYALQSRAERLS 571
           EAAGEHGSCSGTHCFAVSFLVMAVVAFVGFLVAAALFFRTRRFYALQSRAERLS
Sbjct: 481 EAAGEHGSCSGTHCFAVSFLVMAVVAFVGFLVAAALFFRTRRFYALQSRAERLS 534

BLAST of Cp4.1LG18g08160 vs. NCBI nr
Match: XP_022988364.1 (uncharacterized protein LOC111485628 [Cucurbita maxima])

HSP 1 Score: 902 bits (2332), Expect = 0.0
Identity = 475/534 (88.95%), Postives = 484/534 (90.64%), Query Frame = 0

Query: 73  MEASGFMASKWIATAFAIWIQCSCGAATFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNA 132
           MEASGFMASKWIATAFAIWIQCSCGAATFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNA
Sbjct: 1   MEASGFMASKWIATAFAIWIQCSCGAATFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNA 60

Query: 133 GIISGFLCSAVTSTFPRRPFVGPWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCFF 192
           GIISGFLCSAVTS  PRRPFVGPWMVYAAGA+QCFLGYIFMWAAVSGLIDRPSVPVMCFF
Sbjct: 61  GIISGFLCSAVTSPIPRRPFVGPWMVYAAGAIQCFLGYIFMWAAVSGLIDRPSVPVMCFF 120

Query: 193 MLLASHAQTFFSTANVVTGVHNFKNYSGTIVGIMKN----------QAIFSSC------- 252
           MLLASHAQTFFSTANVVTGVHNFK+YSGTIVGIMK           Q   + C       
Sbjct: 121 MLLASHAQTFFSTANVVTGVHNFKHYSGTIVGIMKGYLGLSGALVIQVYNTVCNGEPSNF 180

Query: 253 ----------------W--RIDRTESKNERKHLDSLSAHAVIVAFYLMAVIILDNTFSLP 312
                           W  RIDRTES+NERKHL+SLSAHAVIVAFYLMAVIILDNTFSL 
Sbjct: 181 LLLLAVLPTLLSIMFMWFIRIDRTESRNERKHLNSLSAHAVIVAFYLMAVIILDNTFSLS 240

Query: 313 SSTRSFTFSILLILLSAPLGIAINARREDLKGTEKTHVLNKSKSVDTEDLVEYHELPRDD 372
           SSTRSFTFSILLILLSAPLGIAINARREDLK TEK HVLNKSKSVDTEDLVEYHELPRD+
Sbjct: 241 SSTRSFTFSILLILLSAPLGIAINARREDLKRTEKPHVLNKSKSVDTEDLVEYHELPRDE 300

Query: 373 GQIMAVASSSTPQAMNVLEALCTINFWLLFTAMVCGMGSGLATINNMTQLGQSLGYTVTE 432
            QIMAVASSSTPQAMNVL+ALCTINFWLLFTAMVCGMGSGLATINNMTQLGQSLGYTVTE
Sbjct: 301 RQIMAVASSSTPQAMNVLDALCTINFWLLFTAMVCGMGSGLATINNMTQLGQSLGYTVTE 360

Query: 433 TKTFVSLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLLIMSGGHLVIASSFSGNL 492
           TKTFVSLWSIWNFLGRFGAGYASDYLLHSYG ARPLLMAITLLIMSGGHLVIASSFSGNL
Sbjct: 361 TKTFVSLWSIWNFLGRFGAGYASDYLLHSYGYARPLLMAITLLIMSGGHLVIASSFSGNL 420

Query: 493 YVGSTLVGICYGSQWSLMPSITSDIFGIEHMGTIFNAIATASPLGSYVFSVKVIGYIYDR 552
           YVGSTLVGICYGSQWSLMPS+TSDIFG+EHMGTIFNAIATASPLGSYVFSVKVIGYIYDR
Sbjct: 421 YVGSTLVGICYGSQWSLMPSVTSDIFGLEHMGTIFNAIATASPLGSYVFSVKVIGYIYDR 480

Query: 553 EAAGEHGSCSGTHCFAVSFLVMAVVAFVGFLVAAALFFRTRRFYALQSRAERLS 571
           E AGE GSCSGTHCFAVSFLVMAVVAFVGFLVAAALF RTRRFYALQSRAERLS
Sbjct: 481 EGAGEDGSCSGTHCFAVSFLVMAVVAFVGFLVAAALFLRTRRFYALQSRAERLS 534

BLAST of Cp4.1LG18g08160 vs. NCBI nr
Match: XP_008453166.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo] >KAA0057917.1 protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa] >TYJ98605.1 protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 760 bits (1962), Expect = 4.83e-271
Identity = 403/543 (74.22%), Postives = 443/543 (81.58%), Query Frame = 0

Query: 73  MEASGFMASKWIATAFAIWIQCSCGAA-TFSIYSSALKSSQGYDQSTLGTVSVFKDIGTN 132
           MEAS FM +KW AT   IWIQC CGA+ TFSIYSSALKS+Q YDQSTL TVSVFKDIG N
Sbjct: 1   MEASNFMHNKWTATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 133 AGIISGFLCSAVTSTFPRRPFVGPWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCF 192
           AGIISGFL SAVT   PRR F GPWMV+AAGA+Q FLGYIF+WAAVSG+IDRP VP MCF
Sbjct: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 193 FMLLASHAQTFFSTANVVTGVHNFKNYSGTIVGIMKN----------QAIFSSC------ 252
           FM LA+HAQTFF+TANVVTGVHNF NYSGTIVGIMK           Q   ++C      
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNADPSN 180

Query: 253 -----------------W--RIDRTESKNERKHLDSLSAHAVIVAFYLMAVIILDNTFSL 312
                            W  RID+TESKNE KHL+SLSA AVIVAFYLM VIIL+N FSL
Sbjct: 181 FLLMLAVLPTVLSIMFMWFVRIDKTESKNEMKHLNSLSALAVIVAFYLMVVIILNNAFSL 240

Query: 313 PSSTRSFTFSILLILLSAPLGIAINARREDLKG------TEKTHVLNKSKSVDTEDLVEY 372
            S TR FTFSILLILL+APLGIAINA++ED +G      TE++HV+NK + +D ED VEY
Sbjct: 241 SSWTRYFTFSILLILLAAPLGIAINAQKEDFRGSPPSLITEESHVVNKPEPIDAEDSVEY 300

Query: 373 HELPRDDGQIMAVASSSTPQAMNVLEALCTINFWLLFTAMVCGMGSGLATINNMTQLGQS 432
           HELPR++ QIM V++S  PQ MNVLEA+ TINFWLLF AMVCGMGSGLATINNM+QLGQS
Sbjct: 301 HELPREEDQIMVVSNSRAPQPMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360

Query: 433 LGYTVTETKTFVSLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLLIMSGGHLVIA 492
           LGYT TETKTFVSLWSIWNFLGRFGAGY SD+L H+YG ARPLLMAITLLIMSGGH+VIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIA 420

Query: 493 SSFSGNLYVGSTLVGICYGSQWSLMPSITSDIFGIEHMGTIFNAIATASPLGSYVFSVKV 552
           S FSGNLYVGS LVGICYGSQWSLMP+ITS+IFG+EHMGTIFN IA ASPLGSY+FSV+V
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 553 IGYIYDREAAGEHGSCSGTHCFAVSFLVMAVVAFVGFLVAAALFFRTRRFY--ALQSRAE 571
           IGYIYDREAAGEHG+CSGTHCF VSFLVMA+VAF+GFLVAAALFFRTRRFY  ALQ R E
Sbjct: 481 IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLALQRRVE 540

BLAST of Cp4.1LG18g08160 vs. ExPASy TrEMBL
Match: A0A6J1E3N1 (uncharacterized protein LOC111429640 OS=Cucurbita moschata OX=3662 GN=LOC111429640 PE=4 SV=1)

HSP 1 Score: 925 bits (2391), Expect = 0.0
Identity = 486/534 (91.01%), Postives = 491/534 (91.95%), Query Frame = 0

Query: 73  MEASGFMASKWIATAFAIWIQCSCGAATFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNA 132
           MEASGFMASKWIATAFAIWIQCSCGAATFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNA
Sbjct: 1   MEASGFMASKWIATAFAIWIQCSCGAATFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNA 60

Query: 133 GIISGFLCSAVTSTFPRRPFVGPWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCFF 192
           GIISGFLCSAVTS FPRRPFVGPWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCFF
Sbjct: 61  GIISGFLCSAVTSPFPRRPFVGPWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCFF 120

Query: 193 MLLASHAQTFFSTANVVTGVHNFKNYSGTIVGIMKN----------QAIFSSC------- 252
           MLLASHAQTFFSTANVVTGVHNFK+YSGTIVGIMK           Q   + C       
Sbjct: 121 MLLASHAQTFFSTANVVTGVHNFKHYSGTIVGIMKGYLGLSGALVIQVYNTICNGEPSNF 180

Query: 253 ----------------W--RIDRTESKNERKHLDSLSAHAVIVAFYLMAVIILDNTFSLP 312
                           W  RIDRTES+NERKHLDSLSAHAVIVAFYLMAVIILDNTFSLP
Sbjct: 181 LLLLAVLPTLLSIMFMWFIRIDRTESRNERKHLDSLSAHAVIVAFYLMAVIILDNTFSLP 240

Query: 313 SSTRSFTFSILLILLSAPLGIAINARREDLKGTEKTHVLNKSKSVDTEDLVEYHELPRDD 372
           SSTRSFTFSILLILLSAPLGIAINARREDLK TEKTHVLNKSKSVDTEDLVEYHELPRD+
Sbjct: 241 SSTRSFTFSILLILLSAPLGIAINARREDLKVTEKTHVLNKSKSVDTEDLVEYHELPRDE 300

Query: 373 GQIMAVASSSTPQAMNVLEALCTINFWLLFTAMVCGMGSGLATINNMTQLGQSLGYTVTE 432
            QIMAVASSSTPQAMNVLEALCTINFWLLFTAMVCGMGSGLATINNMTQLGQSLGYTVTE
Sbjct: 301 RQIMAVASSSTPQAMNVLEALCTINFWLLFTAMVCGMGSGLATINNMTQLGQSLGYTVTE 360

Query: 433 TKTFVSLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLLIMSGGHLVIASSFSGNL 492
           TKTFVSLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLLIMSGGHLVIASSFSGNL
Sbjct: 361 TKTFVSLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLLIMSGGHLVIASSFSGNL 420

Query: 493 YVGSTLVGICYGSQWSLMPSITSDIFGIEHMGTIFNAIATASPLGSYVFSVKVIGYIYDR 552
           YVGSTLVGICYGSQWSLMPSITSDIFG+EHMGTIFNAIATASPLGSYVFSVKVIGYIYDR
Sbjct: 421 YVGSTLVGICYGSQWSLMPSITSDIFGLEHMGTIFNAIATASPLGSYVFSVKVIGYIYDR 480

Query: 553 EAAGEHGSCSGTHCFAVSFLVMAVVAFVGFLVAAALFFRTRRFYALQSRAERLS 571
           EAAGEHGSCSGTHCFAVSFLVMAVVAFVGFLVAAALFFRTRRFYALQSRAERLS
Sbjct: 481 EAAGEHGSCSGTHCFAVSFLVMAVVAFVGFLVAAALFFRTRRFYALQSRAERLS 534

BLAST of Cp4.1LG18g08160 vs. ExPASy TrEMBL
Match: A0A6J1JLB9 (uncharacterized protein LOC111485628 OS=Cucurbita maxima OX=3661 GN=LOC111485628 PE=4 SV=1)

HSP 1 Score: 902 bits (2332), Expect = 0.0
Identity = 475/534 (88.95%), Postives = 484/534 (90.64%), Query Frame = 0

Query: 73  MEASGFMASKWIATAFAIWIQCSCGAATFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNA 132
           MEASGFMASKWIATAFAIWIQCSCGAATFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNA
Sbjct: 1   MEASGFMASKWIATAFAIWIQCSCGAATFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNA 60

Query: 133 GIISGFLCSAVTSTFPRRPFVGPWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCFF 192
           GIISGFLCSAVTS  PRRPFVGPWMVYAAGA+QCFLGYIFMWAAVSGLIDRPSVPVMCFF
Sbjct: 61  GIISGFLCSAVTSPIPRRPFVGPWMVYAAGAIQCFLGYIFMWAAVSGLIDRPSVPVMCFF 120

Query: 193 MLLASHAQTFFSTANVVTGVHNFKNYSGTIVGIMKN----------QAIFSSC------- 252
           MLLASHAQTFFSTANVVTGVHNFK+YSGTIVGIMK           Q   + C       
Sbjct: 121 MLLASHAQTFFSTANVVTGVHNFKHYSGTIVGIMKGYLGLSGALVIQVYNTVCNGEPSNF 180

Query: 253 ----------------W--RIDRTESKNERKHLDSLSAHAVIVAFYLMAVIILDNTFSLP 312
                           W  RIDRTES+NERKHL+SLSAHAVIVAFYLMAVIILDNTFSL 
Sbjct: 181 LLLLAVLPTLLSIMFMWFIRIDRTESRNERKHLNSLSAHAVIVAFYLMAVIILDNTFSLS 240

Query: 313 SSTRSFTFSILLILLSAPLGIAINARREDLKGTEKTHVLNKSKSVDTEDLVEYHELPRDD 372
           SSTRSFTFSILLILLSAPLGIAINARREDLK TEK HVLNKSKSVDTEDLVEYHELPRD+
Sbjct: 241 SSTRSFTFSILLILLSAPLGIAINARREDLKRTEKPHVLNKSKSVDTEDLVEYHELPRDE 300

Query: 373 GQIMAVASSSTPQAMNVLEALCTINFWLLFTAMVCGMGSGLATINNMTQLGQSLGYTVTE 432
            QIMAVASSSTPQAMNVL+ALCTINFWLLFTAMVCGMGSGLATINNMTQLGQSLGYTVTE
Sbjct: 301 RQIMAVASSSTPQAMNVLDALCTINFWLLFTAMVCGMGSGLATINNMTQLGQSLGYTVTE 360

Query: 433 TKTFVSLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLLIMSGGHLVIASSFSGNL 492
           TKTFVSLWSIWNFLGRFGAGYASDYLLHSYG ARPLLMAITLLIMSGGHLVIASSFSGNL
Sbjct: 361 TKTFVSLWSIWNFLGRFGAGYASDYLLHSYGYARPLLMAITLLIMSGGHLVIASSFSGNL 420

Query: 493 YVGSTLVGICYGSQWSLMPSITSDIFGIEHMGTIFNAIATASPLGSYVFSVKVIGYIYDR 552
           YVGSTLVGICYGSQWSLMPS+TSDIFG+EHMGTIFNAIATASPLGSYVFSVKVIGYIYDR
Sbjct: 421 YVGSTLVGICYGSQWSLMPSVTSDIFGLEHMGTIFNAIATASPLGSYVFSVKVIGYIYDR 480

Query: 553 EAAGEHGSCSGTHCFAVSFLVMAVVAFVGFLVAAALFFRTRRFYALQSRAERLS 571
           E AGE GSCSGTHCFAVSFLVMAVVAFVGFLVAAALF RTRRFYALQSRAERLS
Sbjct: 481 EGAGEDGSCSGTHCFAVSFLVMAVVAFVGFLVAAALFLRTRRFYALQSRAERLS 534

BLAST of Cp4.1LG18g08160 vs. ExPASy TrEMBL
Match: A0A5A7UT75 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G002270 PE=4 SV=1)

HSP 1 Score: 760 bits (1962), Expect = 2.34e-271
Identity = 403/543 (74.22%), Postives = 443/543 (81.58%), Query Frame = 0

Query: 73  MEASGFMASKWIATAFAIWIQCSCGAA-TFSIYSSALKSSQGYDQSTLGTVSVFKDIGTN 132
           MEAS FM +KW AT   IWIQC CGA+ TFSIYSSALKS+Q YDQSTL TVSVFKDIG N
Sbjct: 1   MEASNFMHNKWTATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 133 AGIISGFLCSAVTSTFPRRPFVGPWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCF 192
           AGIISGFL SAVT   PRR F GPWMV+AAGA+Q FLGYIF+WAAVSG+IDRP VP MCF
Sbjct: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 193 FMLLASHAQTFFSTANVVTGVHNFKNYSGTIVGIMKN----------QAIFSSC------ 252
           FM LA+HAQTFF+TANVVTGVHNF NYSGTIVGIMK           Q   ++C      
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNADPSN 180

Query: 253 -----------------W--RIDRTESKNERKHLDSLSAHAVIVAFYLMAVIILDNTFSL 312
                            W  RID+TESKNE KHL+SLSA AVIVAFYLM VIIL+N FSL
Sbjct: 181 FLLMLAVLPTVLSIMFMWFVRIDKTESKNEMKHLNSLSALAVIVAFYLMVVIILNNAFSL 240

Query: 313 PSSTRSFTFSILLILLSAPLGIAINARREDLKG------TEKTHVLNKSKSVDTEDLVEY 372
            S TR FTFSILLILL+APLGIAINA++ED +G      TE++HV+NK + +D ED VEY
Sbjct: 241 SSWTRYFTFSILLILLAAPLGIAINAQKEDFRGSPPSLITEESHVVNKPEPIDAEDSVEY 300

Query: 373 HELPRDDGQIMAVASSSTPQAMNVLEALCTINFWLLFTAMVCGMGSGLATINNMTQLGQS 432
           HELPR++ QIM V++S  PQ MNVLEA+ TINFWLLF AMVCGMGSGLATINNM+QLGQS
Sbjct: 301 HELPREEDQIMVVSNSRAPQPMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360

Query: 433 LGYTVTETKTFVSLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLLIMSGGHLVIA 492
           LGYT TETKTFVSLWSIWNFLGRFGAGY SD+L H+YG ARPLLMAITLLIMSGGH+VIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIA 420

Query: 493 SSFSGNLYVGSTLVGICYGSQWSLMPSITSDIFGIEHMGTIFNAIATASPLGSYVFSVKV 552
           S FSGNLYVGS LVGICYGSQWSLMP+ITS+IFG+EHMGTIFN IA ASPLGSY+FSV+V
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 553 IGYIYDREAAGEHGSCSGTHCFAVSFLVMAVVAFVGFLVAAALFFRTRRFY--ALQSRAE 571
           IGYIYDREAAGEHG+CSGTHCF VSFLVMA+VAF+GFLVAAALFFRTRRFY  ALQ R E
Sbjct: 481 IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLALQRRVE 540

BLAST of Cp4.1LG18g08160 vs. ExPASy TrEMBL
Match: A0A1S3BWC2 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103493962 PE=4 SV=1)

HSP 1 Score: 760 bits (1962), Expect = 2.34e-271
Identity = 403/543 (74.22%), Postives = 443/543 (81.58%), Query Frame = 0

Query: 73  MEASGFMASKWIATAFAIWIQCSCGAA-TFSIYSSALKSSQGYDQSTLGTVSVFKDIGTN 132
           MEAS FM +KW AT   IWIQC CGA+ TFSIYSSALKS+Q YDQSTL TVSVFKDIG N
Sbjct: 1   MEASNFMHNKWTATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 133 AGIISGFLCSAVTSTFPRRPFVGPWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCF 192
           AGIISGFL SAVT   PRR F GPWMV+AAGA+Q FLGYIF+WAAVSG+IDRP VP MCF
Sbjct: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 193 FMLLASHAQTFFSTANVVTGVHNFKNYSGTIVGIMKN----------QAIFSSC------ 252
           FM LA+HAQTFF+TANVVTGVHNF NYSGTIVGIMK           Q   ++C      
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNADPSN 180

Query: 253 -----------------W--RIDRTESKNERKHLDSLSAHAVIVAFYLMAVIILDNTFSL 312
                            W  RID+TESKNE KHL+SLSA AVIVAFYLM VIIL+N FSL
Sbjct: 181 FLLMLAVLPTVLSIMFMWFVRIDKTESKNEMKHLNSLSALAVIVAFYLMVVIILNNAFSL 240

Query: 313 PSSTRSFTFSILLILLSAPLGIAINARREDLKG------TEKTHVLNKSKSVDTEDLVEY 372
            S TR FTFSILLILL+APLGIAINA++ED +G      TE++HV+NK + +D ED VEY
Sbjct: 241 SSWTRYFTFSILLILLAAPLGIAINAQKEDFRGSPPSLITEESHVVNKPEPIDAEDSVEY 300

Query: 373 HELPRDDGQIMAVASSSTPQAMNVLEALCTINFWLLFTAMVCGMGSGLATINNMTQLGQS 432
           HELPR++ QIM V++S  PQ MNVLEA+ TINFWLLF AMVCGMGSGLATINNM+QLGQS
Sbjct: 301 HELPREEDQIMVVSNSRAPQPMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360

Query: 433 LGYTVTETKTFVSLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLLIMSGGHLVIA 492
           LGYT TETKTFVSLWSIWNFLGRFGAGY SD+L H+YG ARPLLMAITLLIMSGGH+VIA
Sbjct: 361 LGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIA 420

Query: 493 SSFSGNLYVGSTLVGICYGSQWSLMPSITSDIFGIEHMGTIFNAIATASPLGSYVFSVKV 552
           S FSGNLYVGS LVGICYGSQWSLMP+ITS+IFG+EHMGTIFN IA ASPLGSY+FSV+V
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 553 IGYIYDREAAGEHGSCSGTHCFAVSFLVMAVVAFVGFLVAAALFFRTRRFY--ALQSRAE 571
           IGYIYDREAAGEHG+CSGTHCF VSFLVMA+VAF+GFLVAAALFFRTRRFY  ALQ R E
Sbjct: 481 IGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLALQRRVE 540

BLAST of Cp4.1LG18g08160 vs. ExPASy TrEMBL
Match: A0A0A0LUE8 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G008470 PE=4 SV=1)

HSP 1 Score: 749 bits (1935), Expect = 2.98e-267
Identity = 396/543 (72.93%), Postives = 440/543 (81.03%), Query Frame = 0

Query: 73  MEASGFMASKWIATAFAIWIQCSCGAA-TFSIYSSALKSSQGYDQSTLGTVSVFKDIGTN 132
           M+ S FM++KWIAT   IWIQC CGA+ TFSIYSSALKS+Q YDQSTL TVSVFKDIG N
Sbjct: 1   MDPSNFMSNKWIATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 133 AGIISGFLCSAVTSTFPRRPFVGPWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCF 192
           AGIISGFL SAVT   PRR F GPWMV+AAGA+Q FLGYIF+WAAVSG+IDRP VP MCF
Sbjct: 61  AGIISGFLYSAVTPFNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 193 FMLLASHAQTFFSTANVVTGVHNFKNYSGTIVGIMKN----------QAIFSSC------ 252
           FM LA+HAQTFF+TANVVTGVHNF NYSGTIVGIMK           Q   ++C      
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNEDPSN 180

Query: 253 -----------------W--RIDRTESKNERKHLDSLSAHAVIVAFYLMAVIILDNTFSL 312
                            W  RID+TES NE KHL+SLSA AVIVAFYLM VIIL+N FSL
Sbjct: 181 FLLMLAVLPTVLSVMFMWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNAFSL 240

Query: 313 PSSTRSFTFSILLILLSAPLGIAINARREDLKGT------EKTHVLNKSKSVDTEDLVEY 372
            S TR FTFSILLILL+APLGIAINA++ED +G+      EK+HV+NK +S+D ED VEY
Sbjct: 241 SSWTRYFTFSILLILLAAPLGIAINAQKEDFRGSSSSLIAEKSHVVNKPESIDAEDSVEY 300

Query: 373 HELPRDDGQIMAVASSSTPQAMNVLEALCTINFWLLFTAMVCGMGSGLATINNMTQLGQS 432
           HELPR++ QIM V+++  PQ MNVLEA+ TINFWLLF AMVCGMGSGLATINNM+QLGQS
Sbjct: 301 HELPREENQIMVVSNTRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQS 360

Query: 433 LGYTVTETKTFVSLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLLIMSGGHLVIA 492
           LGYT TETKTFVSLWSIWNFLGRFGAGY SD+L H+YG ARPLLMAITLLIMSGGH+VIA
Sbjct: 361 LGYTETETKTFVSLWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIA 420

Query: 493 SSFSGNLYVGSTLVGICYGSQWSLMPSITSDIFGIEHMGTIFNAIATASPLGSYVFSVKV 552
           S FSGNLYVGS LVGICYGSQWSLMP+ITS+IFG+EHMGTIFN IA ASPLGSY+FSV+V
Sbjct: 421 SGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRV 480

Query: 553 IGYIYDREAAGEHGSCSGTHCFAVSFLVMAVVAFVGFLVAAALFFRTRRFYAL--QSRAE 571
           IGYIYDREAA EHG+CSG HCF VSF VMA+VAF+GFLVAAALFFRTRRFY L  Q R  
Sbjct: 481 IGYIYDREAAREHGACSGIHCFVVSFFVMAIVAFLGFLVAAALFFRTRRFYQLASQRRVN 540

BLAST of Cp4.1LG18g08160 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 491.5 bits (1264), Expect = 9.1e-139
Identity = 274/526 (52.09%), Postives = 352/526 (66.92%), Query Frame = 0

Query: 79  MASKWIATAFAIWIQCSCGAA-TFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNAGIISG 138
           + +KW+A   +IWIQC+ GA+ TF IYS+ LKS+Q YDQSTL TVSVFKDIG NAG+ SG
Sbjct: 4   LRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVFSG 63

Query: 139 FLCSAVTSTFPR------RPFVGPWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCF 198
            L +  TS   R          GPW+V A GA+QCF GY  +WA+V+GLI +P VP+MC 
Sbjct: 64  LLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLMCL 123

Query: 199 FMLLASHAQTFFSTANVVTGVHNFKNYSGTIVGIMK-----NQAIFSSCW---------- 258
           FM LA+ +QTFF+TANVV+ V NF +Y GT VGIMK     + AI    +          
Sbjct: 124 FMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDPAS 183

Query: 259 --------------------RIDRTESKNERKHLDSLSAHAVIVAFYLMAVIILDNTFSL 318
                               RI  T   +++KHL+ LSA ++I+A YLM +IIL NTF L
Sbjct: 184 FILLLAVTPTVLSLLVMPLVRIYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKNTFGL 243

Query: 319 PSSTRSFTFSILLILLSAPLGIAINARREDLKGTEKTHVLNKSKSVDT-EDLVEYHELPR 378
            S     T   LL++L+ PL IA  A+R+   G EKT   + S  + + +     ++   
Sbjct: 244 SSWANIVTLVCLLVMLALPLLIARRAQRD---GMEKTVPHDYSPLISSPKATTSGNQSSE 303

Query: 379 DDGQIMAVASSSTPQAMNVLEALCTINFWLLFTAMVCGMGSGLATINNMTQLGQSLGYTV 438
            D ++ A  S +    +N+L+A+  ++FWLLF AM+CGMGSGL+TINN+ Q+G+SL Y+ 
Sbjct: 304 GDSKVEAGLSEN----LNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQIGESLRYSS 363

Query: 439 TETKTFVSLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLLIMSGGHLVIASSFSG 498
            E  + VSLWSIWNFLGRFGAGYASD LLH  G  RPLLMA TL  MS GHL+IAS F G
Sbjct: 364 VEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHLIIASGFQG 423

Query: 499 NLYVGSTLVGICYGSQWSLMPSITSDIFGIEHMGTIFNAIATASPLGSYVFSVKVIGYIY 558
           NLYVGS +VG+CYGSQWSLMP+ITS++FGI HMGTIFN I+ ASP+GSY+FSV++IGYIY
Sbjct: 424 NLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIFSVRLIGYIY 483

Query: 559 DREAAGEHGSCSGTHCFAVSFLVMAVVAFVGFLVAAALFFRTRRFY 562
           D+ A+GE  +C G+HCF +SF++MA VAF GFLVA  LFFRT+  Y
Sbjct: 484 DKTASGEGNTCYGSHCFRLSFIIMASVAFFGFLVAIVLFFRTKTLY 522

BLAST of Cp4.1LG18g08160 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 474.2 bits (1219), Expect = 1.5e-133
Identity = 257/527 (48.77%), Postives = 346/527 (65.65%), Query Frame = 0

Query: 75  ASGFMASKWIATAFAIWIQCSCGAA-TFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNAG 134
           A+  + +KW+A   +IWIQCS G + TF IYS+ LKS+Q YDQSTL TVSVFKDIG N G
Sbjct: 2   ATEILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVG 61

Query: 135 IISGFLCSAVTSTFPRRP----FVGPWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVM 194
           ++SG + +A T    RR       GPW+V   GA+  F GY  MWA+V+GLI RP VPVM
Sbjct: 62  VLSGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVM 121

Query: 195 CFFMLLASHAQTFFSTANVVTGVHNFKNYSGTIVGIMKNQAIFSSCW------------- 254
           C FM +A+ + TF +TANVV+ + NF +Y GT VGIMK     S                
Sbjct: 122 CLFMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDP 181

Query: 255 ----------------------RIDRTESKNERKHLDSLSAHAVIVAFYLMAVIILDNTF 314
                                 R+ +T + +E+KHLD LS  ++I+A YLM  IIL +T 
Sbjct: 182 KTFILLLAIVPSLLSVLVMPLVRVYKTSTVDEKKHLDGLSTLSLIIAAYLMITIILKSTL 241

Query: 315 SLPSSTRSFTFSILLILLSAPLGIAINARREDLKGTEKTHVLNKSKSVDTEDLVEYHELP 374
           SLPS   + T ++LL+LLS+PL +A+ A R+ ++         K  S     LV+  E  
Sbjct: 242 SLPSWANAVTLAVLLVLLSSPLLVAVRAHRDSIE---------KPLSSVYSPLVDNLE-A 301

Query: 375 RDDGQIMAVASSSTPQAMNVLEALCTINFWLLFTAMVCGMGSGLATINNMTQLGQSLGYT 434
              G+I+ +      +++N+L+A+C ++FWLLF AM+CGMGSG++TINN+ Q+G+SL YT
Sbjct: 302 TTSGEILML---DEDKSLNLLQAMCNVDFWLLFLAMICGMGSGISTINNIRQIGESLRYT 361

Query: 435 VTETKTFVSLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLLIMSGGHLVIASSFS 494
             E  + ++LW+IWNF+GRFG GY SD+LLH  G  RPLLMA TL  M+ GHL+IAS F 
Sbjct: 362 SVEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLGTMTIGHLIIASGFQ 421

Query: 495 GNLYVGSTLVGICYGSQWSLMPSITSDIFGIEHMGTIFNAIATASPLGSYVFSVKVIGYI 554
           GNLY GS +VGICYGSQWSLMP+ITS++FG++HMGTI+N I+ ASP+GSY+FSV++IGYI
Sbjct: 422 GNLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIASPMGSYIFSVRLIGYI 481

Query: 555 YDREAAGEHGSCSGTHCFAVSFLVMAVVAFVGFLVAAALFFRTRRFY 562
           YDR   GE  +C G HCF ++++V+A VAF+GFLV+  L FRT+  Y
Sbjct: 482 YDRTIIGEGNTCYGPHCFRLAYVVIASVAFLGFLVSCVLVFRTKTIY 515

BLAST of Cp4.1LG18g08160 vs. TAIR 10
Match: AT2G34350.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 458.8 bits (1179), Expect = 6.6e-129
Identity = 258/531 (48.59%), Postives = 331/531 (62.34%), Query Frame = 0

Query: 78  FMASKWIATAFAIWIQCSCGAA-TFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNAGIIS 137
           F  +KW+A A +IWIQ   GA+ TF IYSS LKSSQ YDQSTL TVSV+KDIG N GI+S
Sbjct: 3   FANTKWVAAAASIWIQSFSGASYTFGIYSSVLKSSQSYDQSTLDTVSVYKDIGANVGILS 62

Query: 138 GFLCSAVTSTFPRRP--FVGPWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCFFML 197
           G   +AV S        F GPW+V   G +Q F+GY F+W A SG+I RP V +MC FM 
Sbjct: 63  GLFYTAVASRKSGNGGFFSGPWLVIFVGLLQWFVGYGFIWMATSGVIPRPPVAMMCLFMF 122

Query: 198 LASHAQTFFSTANVVTGVHNFKNYSGTIVGIMKNQAIFSSCWRIDR-------------- 257
            A H Q FF+TA VVT V NF +Y GT VGIMK     S    +                
Sbjct: 123 FAGHCQPFFNTAIVVTAVRNFSDYGGTAVGIMKGYLGLSGAILVQMYHIFCGGDPRNYIL 182

Query: 258 ---------------------TESKNERKHLDSLSAHAVIVAFYLMAVIILDNTFSLPSS 317
                                T    ++KHL+ LSA ++I+  YLM VI+++N   +   
Sbjct: 183 LLAVVPSLLILTLMPFVRTYDTVIAGDKKHLNGLSAISLIIVTYLMVVILVENIIGMSMP 242

Query: 318 TRSFTFSILLILLSAPLGIAINARRE--------DLKGTEKTHVLNKSKSVDTEDLVEYH 377
            +  +F+ LL+LL++PL +A+ A+RE        D   TE+T +L+  K   + D+ +  
Sbjct: 243 MKICSFTFLLLLLASPLLVAVRAQREEEHRFLSLDFPVTERTTLLDSPKLNSSSDVKDV- 302

Query: 378 ELPRDDGQIMAVASSSTPQAMNVLEALCTINFWLLFTAMVCGMGSGLATINNMTQLGQSL 437
                               MNVLEA+CT NFWLLF AM+CGMGSGLATINN+ Q+G+SL
Sbjct: 303 ----------------MTNDMNVLEAICTTNFWLLFVAMICGMGSGLATINNIRQMGESL 362

Query: 438 GYTVTETKTFVSLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLLIMSGGHLVIAS 497
            Y+  +  + VSLWSIWNFLGRFG+GY SD  LHS+G  RP+ MAITL +M+ GH+V+AS
Sbjct: 363 RYSTVQLNSLVSLWSIWNFLGRFGSGYISDTYLHSHGWPRPVFMAITLGLMAIGHIVMAS 422

Query: 498 SFSGNLYVGSTLVGICYGSQWSLMPSITSDIFGIEHMGTIFNAIATASPLGSYVFSVKVI 557
              G+LY+GS LVG+ YGSQWSLMP+ITS+IFG+ HMGTIF  I+ ASP+GSY FSVKVI
Sbjct: 423 GLLGSLYIGSLLVGLAYGSQWSLMPTITSEIFGVLHMGTIFYTISIASPVGSYFFSVKVI 482

Query: 558 GYIYDREAAGEHGSCSGTHCFAVSFLVMAVVAFVGFLVAAALFFRTRRFYA 563
           GY+YD+ A+ +  SC G HCF  SFL+MA +A +G LVA  L  RT++FYA
Sbjct: 483 GYLYDKVASEDDHSCYGNHCFRTSFLIMAAMALLGSLVALVLLLRTKKFYA 516

BLAST of Cp4.1LG18g08160 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 454.9 bits (1169), Expect = 9.5e-128
Identity = 256/519 (49.33%), Postives = 329/519 (63.39%), Query Frame = 0

Query: 81  SKWIATAFAIWIQCSCGAA-TFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNAGIISGFL 140
           +KW+A A +IWIQ   GA  TF+IYSS LKSSQ YDQSTL  VSVFKDIG   GIISGFL
Sbjct: 6   TKWVAAAASIWIQSFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIISGFL 65

Query: 141 CSAVTSTFPRRPFVGPWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCFFMLLASHA 200
            +A+TS    R F GPW+V   G VQ F+G+ F+WA+V GLI  P VP+MC F+ LA H+
Sbjct: 66  YTAMTS--KSRGFGGPWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAGHS 125

Query: 201 QTFFSTANVVTGVHNFKNYSGTIVGIMKN----------QAIFSSCW------------- 260
             FF+TANVVT   NF  Y GT VGIM+           Q   + C              
Sbjct: 126 LPFFNTANVVTAARNFSQYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFILLLA 185

Query: 261 --------------RIDRTESKNERKHLDSLSAHAVIVAFYLMAVIILDNTFSLPSSTRS 320
                         R+  T + +++KHLD LSA ++I+A YLM VI ++N   L  S + 
Sbjct: 186 IVPTLVMFLAMPFVRVYETVTISDKKHLDGLSAISMIIAAYLMVVITVENVLGLSRSMQI 245

Query: 321 FTFSILLILLSAPLGIAINARREDLKGTEKTHVLNKSKSVDTEDLVEYHELPRDDGQIMA 380
           F+F ++L+LL++PL +A+ A R      EK   L+   S+D   L     L      I  
Sbjct: 246 FSFILVLLLLASPLLVAVRALR------EKRQTLS---SLDGPVLDTSALLDPPSSNIFP 305

Query: 381 VASSSTPQAMNVLEALCTINFWLLFTAMVCGMGSGLATINNMTQLGQSLGYTVTETKTFV 440
                  +  N+LEA+ T+NFWLLF AM+CGMGSG AT+NNM Q+G+SL Y+  +  + V
Sbjct: 306 DGDHLVAEDSNILEAMSTVNFWLLFLAMLCGMGSGFATVNNMRQIGESLRYSSVQLNSLV 365

Query: 441 SLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLLIMSGGHLVIASSFSGNLYVGST 500
           SLWSIWNFLGRFGAGY SD  LH +   RP+ MAITL +M+ GH+++AS   G+LY GS 
Sbjct: 366 SLWSIWNFLGRFGAGYVSDTFLHKHSWPRPIFMAITLGVMAIGHIIVASGVQGSLYAGSV 425

Query: 501 LVGICYGSQWSLMPSITSDIFGIEHMGTIFNAIATASPLGSYVFSVKVIGYIYDREAAGE 560
           L+G+ YGSQWSLMP+ITS+IFGI HMGTI+  I+ A P+GSY+ SVKVIGY YD+ A+ +
Sbjct: 426 LIGMAYGSQWSLMPTITSEIFGIRHMGTIYFTISIAGPIGSYILSVKVIGYFYDKVASED 485

Query: 561 HGSCSGTHCFAVSFLVMAVVAFVGFLVAAALFFRTRRFY 562
             SC G+ CF  SF++MA VA  G LVA+ LFFRT +FY
Sbjct: 486 DNSCFGSQCFRTSFMIMASVALFGSLVASVLFFRTHKFY 513

BLAST of Cp4.1LG18g08160 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 263.8 bits (673), Expect = 3.1e-70
Identity = 177/550 (32.18%), Postives = 282/550 (51.27%), Query Frame = 0

Query: 79  MASKWIATAFAIWIQCSCGAA-TFSIYSSALKSSQGYDQSTLGTVSVFKDIGTNAGIISG 138
           +  +W     ++ I  + GA   F IYS  +K + GYDQ+TL  +S FKD+G N G+++G
Sbjct: 18  LTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVLAG 77

Query: 139 FLCSAVTSTFPRRPFVGPWMVYAAGAVQCFLGYIFMWAAVSGLIDRPSVPVMCFFMLLAS 198
            L + VT          PW +   GA+  F GY  +W AV+  I +P V  MC ++ + +
Sbjct: 78  LL-NEVTP---------PWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGA 137

Query: 199 HAQTFFSTANVVTGVHNFKNYSGTIVGIMK-----NQAIFSSCWRI----DRTE------ 258
           ++Q+F +T ++VT V NF    G ++GI+K     + AI +  +R     D  E      
Sbjct: 138 NSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIG 197

Query: 259 ---------------------SKNERKHLDSLSAHAVIVAFYLMAVIILDNTFSLPSSTR 318
                                  NE K   +    ++ +A +LM VII++       S  
Sbjct: 198 WLPAIVSFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEF 257

Query: 319 SFTFSILLILLSAPLGIAINARREDLKGTEKTHVLNKSKSVDT---EDLVEYHELPRDDG 378
             + +++++LL  P+ + I   ++  K  EK   LN    ++    +  ++  E   DDG
Sbjct: 258 GGSAAVVIVLLLLPIIVVILEEKKLWK--EKQVALNDPAPINVVTEKPKLDSSEFKDDDG 317

Query: 379 QIM--AVASSSTPQA-------------MNVLEALCTINFWLLFTAMVCGMGSGLATINN 438
           +     V    TP                 +L+AL +++  +LF A +CG+G  L  I+N
Sbjct: 318 EESKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDN 377

Query: 439 MTQLGQSLGYTVTETKTFVSLWSIWNFLGRFGAGYASDYLLHSYGCARPLLMAITLLIMS 498
           + Q+G SLGY      TFVSL SIWN+ GR  +G  S+  L  Y   RPL++ + LL+  
Sbjct: 378 LGQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSC 437

Query: 499 GGHLVIASSFSGNLYVGSTLVGICYGSQWSLMPSITSDIFGIEHMGTIFNAIATASPLGS 558
            GHL+IA +  G LYV S ++G C+G+QW L+ +I S+IFG+++  T++N  + ASP+GS
Sbjct: 438 AGHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGS 497

Query: 559 YVFSVKVIGYIYDREAA------------GEHGSCSGTHCFAVSFLVMAVVAFVGFLVAA 562
           Y+ +V+V GY+YD EA             G+  +C GT CF +SF+++A V   G LV+ 
Sbjct: 498 YLLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSM 555

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I9E11.5e-2125.55Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Q6CGU81.1e-0829.34Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=28... [more]
Q6BN116.4e-0427.38Probable transporter MCH1 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 ... [more]
Q4WVT38.4e-0429.75Probable transporter mch1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 ... [more]
Match NameE-valueIdentityDescription
KAG6589488.10.091.08Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023515641.10.092.32uncharacterized protein LOC111779750 [Cucurbita pepo subsp. pepo][more]
XP_022921338.10.091.01uncharacterized protein LOC111429640 [Cucurbita moschata][more]
XP_022988364.10.088.95uncharacterized protein LOC111485628 [Cucurbita maxima][more]
XP_008453166.14.83e-27174.22PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo] >KAA0057917.1 prote... [more]
Match NameE-valueIdentityDescription
A0A6J1E3N10.091.01uncharacterized protein LOC111429640 OS=Cucurbita moschata OX=3662 GN=LOC1114296... [more]
A0A6J1JLB90.088.95uncharacterized protein LOC111485628 OS=Cucurbita maxima OX=3661 GN=LOC111485628... [more]
A0A5A7UT752.34e-27174.22Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BWC22.34e-27174.22protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103493962 PE=4 ... [more]
A0A0A0LUE82.98e-26772.93Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G00847... [more]
Match NameE-valueIdentityDescription
AT1G74780.19.1e-13952.09Nodulin-like / Major Facilitator Superfamily protein [more]
AT1G18940.11.5e-13348.77Nodulin-like / Major Facilitator Superfamily protein [more]
AT2G34350.16.6e-12948.59Nodulin-like / Major Facilitator Superfamily protein [more]
AT2G34355.19.5e-12849.33Major facilitator superfamily protein [more]
AT2G39210.13.1e-7032.18Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 82..228
e-value: 3.6E-33
score: 115.1
coord: 239..299
e-value: 2.1E-6
score: 27.4
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 355..564
e-value: 1.3E-9
score: 39.7
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 344..559
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 365..568
e-value: 7.8E-12
score: 44.8
NoneNo IPR availablePANTHERPTHR21576:SF22F25A4.25 PROTEINcoord: 79..229
coord: 235..564
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 235..564
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 79..229
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 81..546
e-value: 2.58068E-102
score: 312.649

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG18g08160.1Cp4.1LG18g08160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity