Cp4.1LG18g06440 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG18g06440 vs. ExPASy Swiss-Prot
Match: Q84TI7 (Sodium transporter HKT1 OS=Arabidopsis thaliana OX=3702 GN=HKT1 PE=1 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 5.0e-122
Identity = 236/482 (48.96%), Postives = 330/482 (68.46%), Query Frame = 0

Query: 27  FLAHLSYFITLSLIGYGSLKLTKTRTSATPNDLNIFFTSVSAATVSSMSVVEMEVFSNFQ 86
           F  +  YF+  S +G+ +LK+TK RT++ P+D ++FFTSVSA TVSSMS V+MEVFSN Q
Sbjct: 22  FFLYFIYFLFFSFLGFLALKITKPRTTSRPHDFDLFFTSVSAITVSSMSTVDMEVFSNTQ 81

Query: 87  LLIITTLMFLGGEVFLSAVSFQLSHRNKQT--NHDIPRTAEFENESATVDE--------- 146
           L+ +T LMFLGGE+F S ++  +S+  K    ++ I       N  +++++         
Sbjct: 82  LIFLTILMFLGGEIFTSFLNLYVSYFTKFVFPHNKIRHILGSYNSDSSIEDRCDVETVTD 141

Query: 147 -----LSISKSSFKVLGRIIIGYLLATNIGGACMIIIYLIMVPHARHVLEIKGINFITFS 206
                + I + + K L  +++ Y L TN+ G+ ++++Y+  V  AR VL  K I+ +TFS
Sbjct: 142 YREGLIKIDERASKCLYSVVLSYHLVTNLVGSVLLLVYVNFVKTARDVLSSKEISPLTFS 201

Query: 207 IFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGCLYPVGLRLVIMAAAKVTG 266
           +F+TVSTF NCGF+PTNENMI+F+KNSGL+ +L  +VL+G  L+P  L L+I    K+T 
Sbjct: 202 VFTTVSTFANCGFVPTNENMIIFRKNSGLIWLLIPQVLMGNTLFPCFLVLLIWGLYKITK 261

Query: 267 KKGWRYILKNDSAMGYSHLLSGVRCRFLAATAAGFIILQLIIFCSLEWNNSDGIWDGMNP 326
           +  + YILKN + MGYSHLLS   C  L  T  GF+I+QL+ FC+ EW +     +GM+ 
Sbjct: 262 RDEYGYILKNHNKMGYSHLLSVRLCVLLGVTVLGFLIIQLLFFCAFEWTSES--LEGMSS 321

Query: 327 YEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLPPYTTFLPL---RTKEKAS 386
           YEK+VGS FQV+NSRHTGE++VD+S +SPAILV+F++MMYLPPYT F+PL   +T EK  
Sbjct: 322 YEKLVGSLFQVVNSRHTGETIVDLSTLSPAILVLFILMMYLPPYTLFMPLTEQKTIEKEG 381

Query: 387 ITTDGNDKRQHLVKFLTFSQLSYLAIFITLICISERDKLRNDPLNFTVLNIAVEVISAYG 446
              D  + ++     L  SQLS+L I I LI I+ER  L+ DP+NF VLNI +EVISAYG
Sbjct: 382 GDDDSENGKKVKKSGLIVSQLSFLTICIFLISITERQNLQRDPINFNVLNITLEVISAYG 441

Query: 447 NVGFSSGYSCKRQV-VADSTCRDAWYGFAGRWSAEGKLILILVMFFGRLKSFSMHGGKAW 489
           NVGF++GYSC+R+V ++D  C+DA YGFAGRWS  GK +LI+VMF+GR K F+   G+AW
Sbjct: 442 NVGFTTGYSCERRVDISDGGCKDASYGFAGRWSPMGKFVLIIVMFYGRFKQFTAKSGRAW 501

BLAST of Cp4.1LG18g06440 vs. ExPASy Swiss-Prot
Match: Q6H501 (Probable cation transporter HKT6 OS=Oryza sativa subsp. japonica OX=39947 GN=HKT6 PE=2 SV=2)

HSP 1 Score: 419.5 bits (1077), Expect = 5.3e-116
Identity = 233/494 (47.17%), Postives = 315/494 (63.77%), Query Frame = 0

Query: 25  NSFLAHLSYFITLSLIGYGSLKLTKTR-TSATPNDLNIFFTSVSAATVSSMSVVEMEVFS 84
           N    H++YF  +S  GYGSLK+ K R  S T  DL++ FTSVSA+TVSSM+ VEME FS
Sbjct: 45  NPLFVHVAYFALISFAGYGSLKVLKPRDKSNTLKDLDVLFTSVSASTVSSMATVEMEDFS 104

Query: 85  NFQLLIITTLMFLGGEVFLSAVSFQLSHRNKQTNHDIPRTAEFENESATVDELSISKSSF 144
           + QL ++T LM +GGEVF S +          T   +       + S  +D  SI+ + F
Sbjct: 105 SAQLWVLTILMLIGGEVFTSMLGIHFMRAEFGTKESV----STRDHSPCIDIESITSTKF 164

Query: 145 -----------------------------KVLGRIIIGYLLATNIGGACMIIIYLIMVPH 204
                                        K LG +++GYLL TN+GG+ +I +YL +VP 
Sbjct: 165 GPSTQGTKVTVSFSELRMENGGHVEPKTIKFLGFVVMGYLLITNLGGSLLIYLYLNLVPS 224

Query: 205 ARHVLEIKGINFITFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGCLY 264
           A  +L+ KGI  I FS+F+ +S+  NCGF P NENMI+F+KNS LL+++  ++L G  L+
Sbjct: 225 AHKILKRKGIGIIVFSVFTAISSVGNCGFTPVNENMIIFQKNSILLLLILPQILAGNTLF 284

Query: 265 PVGLRLVIMAAAKVTGKKGWRYILKNDSAMGYSHLLSGVRCRFLAATAAGFIILQLIIFC 324
              LRL++ +  K+TGKK  RYIL+   A+GY HL+S     +L  T    IILQ ++F 
Sbjct: 285 APCLRLMVWSLEKITGKKDCRYILEYPKAIGYKHLMSTRESVYLTLTVVSLIILQTVLFL 344

Query: 325 SLEWNNSDGIWDGMNPYEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLPPY 384
           SLEW  S    DGM+ Y+K+V + FQ +N+RH GESV D+S +S AILV++ +MMYLP Y
Sbjct: 345 SLEW--SSVALDGMSNYQKIVSALFQSVNARHAGESVTDLSNLSSAILVLYTIMMYLPGY 404

Query: 385 TTFLPLRTKEKASITTDGNDKRQHLVKFLTFSQLSYLAIFITLICISERDKLRNDPLNFT 444
           T+FLP    E +   T+  +KR+ L++   FS +SYLAIF+ LICI+ERD +  DPLNF 
Sbjct: 405 TSFLPRHDGEDSK--TEKINKRKGLLENWIFSHMSYLAIFVMLICITERDSMATDPLNFN 464

Query: 445 VLNIAVEVISAYGNVGFSSGYSCKRQVVADSTCRDAWYGFAGRWSAEGKLILILVMFFGR 489
           V +I  EV+SAYGNVGFS GYSCKR +  D+ C+DA YGFAG+WS  GK ILI+VM FGR
Sbjct: 465 VFSILFEVVSAYGNVGFSVGYSCKRLLNHDARCKDASYGFAGKWSDNGKAILIIVMLFGR 524

BLAST of Cp4.1LG18g06440 vs. ExPASy Swiss-Prot
Match: Q7XPF7 (Probable cation transporter HKT7 OS=Oryza sativa subsp. japonica OX=39947 GN=HKT7 PE=2 SV=2)

HSP 1 Score: 404.8 bits (1039), Expect = 1.4e-111
Identity = 230/498 (46.18%), Postives = 314/498 (63.05%), Query Frame = 0

Query: 23  AANSFLAHLSYFITLSLIGYGSLKLTKTR-TSATPNDLNIFFTSVSAATVSSMSVVEMEV 82
           A  +   H++YF+ +S +GYG L + K R   A P  ++ FFT+VSAATVSSMS VEMEV
Sbjct: 9   AGGALSMHVAYFLAISCLGYGLLGVLKVREPGAAPRRIDRFFTAVSAATVSSMSTVEMEV 68

Query: 83  FSNFQLLIITTLMFLGGEVFLSAV----------SFQLSHRNKQTNH-----------DI 142
           FSN QL+++T LM LGGEVF+S V          S  +    +  +H           D+
Sbjct: 69  FSNGQLVVLTVLMLLGGEVFVSLVGLASKWSKLRSDAMDRSRRVESHGDVALADIDGGDV 128

Query: 143 PRTAEFENESAT----VDELSISKSSFKVLGRIIIGYLLATNIGGACMIIIYLIMVPHAR 202
                   E+A+    +D  ++  ++ + L  I++      ++ GA  +  Y++  P AR
Sbjct: 129 ENPTSSGEEAASRRRPMDADTLRHNAVRALFYIVLAIFAVVHVVGAVAVAAYVLASPGAR 188

Query: 203 HVLEIKGINFITFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGCLYPV 262
             L  K +N  TF++F+TVSTF+NCGF+PTNENM+VFK+++ L ++L  +VL G  L+  
Sbjct: 189 RTLGDKSLNTWTFAVFTTVSTFSNCGFMPTNENMVVFKRDAPLQLLLVPQVLAGNTLFAP 248

Query: 263 GLRLVIMAAAKVTGKKGWRYILKND---SAMGYSHLLSGVRCRFLAATAAGFIILQLIIF 322
            L   + AAA  T ++    + +     +A GY+HL+   RC  LAAT A F+ + + + 
Sbjct: 249 LLAACVWAAAAATRREELVEMAREGGRAAAAGYAHLMPARRCWMLAATVAAFVAVLMALV 308

Query: 323 CSLEWNNSDGIWDGMNPYEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLPP 382
           C +EW    G   GM+P+EKVV + F  +N+RHTGES VD+S+++PAILV+F++MMYLPP
Sbjct: 309 CGMEWG---GALQGMSPWEKVVNALFLAVNARHTGESTVDLSILAPAILVLFVLMMYLPP 368

Query: 383 YTTFLPLRTKEKASITTDGNDKRQ--HLVKFLTFSQLSYLAIFITLICISERDKLRNDPL 442
           YTT+ P    E+ S T D N + Q   L++    SQLSYL IF+  ICI+ER KL+ DPL
Sbjct: 369 YTTWFPF---EENSTTKDSNAENQGIRLLESTLLSQLSYLTIFVIAICITERRKLKEDPL 428

Query: 443 NFTVLNIAVEVISAYGNVGFSSGYSCKRQVVADSTCRDAWYGFAGRWSAEGKLILILVMF 490
           NF+VL+I VEV+SAYGNVGFS GYSC RQ+  D  C D W GF GRWS  GKLILI VMF
Sbjct: 429 NFSVLSIVVEVVSAYGNVGFSMGYSCSRQINPDHLCTDKWTGFVGRWSDSGKLILIFVMF 488

BLAST of Cp4.1LG18g06440 vs. ExPASy Swiss-Prot
Match: A2WNZ9 (Cation transporter HKT8 OS=Oryza sativa subsp. indica OX=39946 GN=HKT8 PE=2 SV=2)

HSP 1 Score: 377.5 bits (968), Expect = 2.3e-103
Identity = 228/531 (42.94%), Postives = 311/531 (58.57%), Query Frame = 0

Query: 24  ANSFLAHLSYFITLSLIGYGSLKLTKTRTSATPN--DLNIFFTSVSAATVSSMSVVEMEV 83
           A+ F   L YF+ +SL+G+  LK    +TS  P   DL++ FTSVSA TVSSM  VEME 
Sbjct: 25  AHPFWLQLLYFLFISLLGFLMLKALPMKTSMVPRPMDLDLIFTSVSATTVSSMVAVEMES 84

Query: 84  FSNFQLLIITTLMFLGGEVFLSAVSFQLSHRNKQT------------------------- 143
           FSN QLL+IT LM LGGEVF S +    ++    +                         
Sbjct: 85  FSNSQLLLITLLMLLGGEVFTSILGLYFTNAKYSSKMIATLPDDDDHGGSGKPPPATTSP 144

Query: 144 ------------------NHDIPRTAEFENE-----------SATVDELSISKSSFK--- 203
                             N     T   E E           + T    S S S+ K   
Sbjct: 145 SSTLVELELAPPMDVVVVNPTTTATTHDEVELGLGRRNKRGCTCTTTHTSSSSSASKTTT 204

Query: 204 --VLGRIIIGYLLATNIGGACMIIIYLIMVPHARHVLEIKGINFITFSIFSTVSTFTNCG 263
             +L  +++GY    ++ G   I++YL  V  A  V+  KGI+  TF+IF+ VSTF NCG
Sbjct: 205 TRLLMFVVMGYHAVVHVAGYTAIVVYLSAVGGAGAVVAGKGISAHTFAIFTVVSTFANCG 264

Query: 264 FIPTNENMIVFKKNSGLLIILAAEVLLGGCLYPVGLRLVIMAAAKVTGKKGWRYIL---K 323
           F+PTNE M+ F+   GLL+++   VLLG  L+PV LRL I A  +VTG      +L   +
Sbjct: 265 FVPTNEGMVSFRSFPGLLLLVMPHVLLGNTLFPVFLRLAIAALERVTGWPELGELLIRRR 324

Query: 324 NDSAMGYSHLLSGVRCRFLAATAAGFIILQLIIFCSLEWNNSDGIWDGMNPYEKVVGSFF 383
                GY HLL   R RFLA T A  ++ QL +FC++EW  SDG+  G+   +K+VG+ F
Sbjct: 325 RGGGEGYHHLLPSSRTRFLALTVAVLVVAQLALFCAMEW-GSDGL-RGLTAGQKLVGALF 384

Query: 384 QVINSRHTGESVVDISLISPAILVVFLVMMYLPPYTTFLPLRTKEKASITTDGND--KRQ 443
             +NSRH+GE V+D+S +S A++V+++VMMYLPPYTTF+P++ K + +    G +     
Sbjct: 385 MAVNSRHSGEMVLDLSTVSSAVVVLYVVMMYLPPYTTFVPVQDKHQQTGAQSGQEGSSSS 444

Query: 444 HLVKFLTFSQLSYLAIFITLICISERDKLRNDPLNFTVLNIAVEVISAYGNVGFSSGYSC 489
            + + L  S LS LAIFI +ICI+ER ++ +DP+N++VLNI VEVISAYGNVGFS+GYSC
Sbjct: 445 SIWQKLLMSPLSCLAIFIVVICITERRQIADDPINYSVLNIVVEVISAYGNVGFSTGYSC 504

BLAST of Cp4.1LG18g06440 vs. ExPASy Swiss-Prot
Match: Q0JNB6 (Cation transporter HKT8 OS=Oryza sativa subsp. japonica OX=39947 GN=HKT8 PE=3 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 2.3e-103
Identity = 228/531 (42.94%), Postives = 311/531 (58.57%), Query Frame = 0

Query: 24  ANSFLAHLSYFITLSLIGYGSLKLTKTRTSATPN--DLNIFFTSVSAATVSSMSVVEMEV 83
           A+ F   L YF+ +SL+G+  LK    +TS  P   DL++ FTSVSA TVSSM  VEME 
Sbjct: 25  AHPFWLQLLYFLFISLLGFLMLKALPMKTSMVPRPMDLDLIFTSVSATTVSSMVAVEMES 84

Query: 84  FSNFQLLIITTLMFLGGEVFLSAVSFQLSHRNKQT------------------------- 143
           FSN QLL+IT LM LGGEVF S +    ++    +                         
Sbjct: 85  FSNSQLLLITLLMLLGGEVFTSILGLYFTNAKYSSKMIATLPDDDDHGGSGKPPPPTTSP 144

Query: 144 ------------------NHDIPRTAEFENE-----------SATVDELSISKSSFK--- 203
                             N     T   E E           + T    S S S+ K   
Sbjct: 145 SSTLVELELAPPMDVVVVNPTTTATTHDEVELGLGRRNKRGCTCTTTHTSSSSSASKTTT 204

Query: 204 --VLGRIIIGYLLATNIGGACMIIIYLIMVPHARHVLEIKGINFITFSIFSTVSTFTNCG 263
             +L  +++GY    ++ G   I++YL  V  A  V+  KGI+  TF+IF+ VSTF NCG
Sbjct: 205 TRLLMFVVMGYHAVVHVAGYTAIVVYLSAVGGAGAVVAGKGISAHTFAIFTVVSTFANCG 264

Query: 264 FIPTNENMIVFKKNSGLLIILAAEVLLGGCLYPVGLRLVIMAAAKVTGKKGWRYIL---K 323
           F+PTNE M+ F+   GLL+++   VLLG  L+PV LRL I A  +VTG      +L   +
Sbjct: 265 FVPTNEGMVSFRSFPGLLLLVMPHVLLGNTLFPVFLRLAIAALERVTGWPELGELLIRRR 324

Query: 324 NDSAMGYSHLLSGVRCRFLAATAAGFIILQLIIFCSLEWNNSDGIWDGMNPYEKVVGSFF 383
                GY HLL   R RFLA T A  ++ QL +FC++EW  SDG+  G+   +K+VG+ F
Sbjct: 325 RGGGEGYHHLLPSSRTRFLALTVAVLVVAQLALFCAMEW-GSDGL-RGLTAGQKLVGALF 384

Query: 384 QVINSRHTGESVVDISLISPAILVVFLVMMYLPPYTTFLPLRTKEKASITTDGND--KRQ 443
             +NSRH+GE V+D+S +S A++V+++VMMYLPPYTTF+P++ K + +    G +     
Sbjct: 385 MAVNSRHSGEMVLDLSTVSSAVVVLYVVMMYLPPYTTFVPVQDKHQQTGAQSGQEGSSSS 444

Query: 444 HLVKFLTFSQLSYLAIFITLICISERDKLRNDPLNFTVLNIAVEVISAYGNVGFSSGYSC 489
            + + L  S LS LAIFI +ICI+ER ++ +DP+N++VLNI VEVISAYGNVGFS+GYSC
Sbjct: 445 SIWQKLLMSPLSCLAIFIVVICITERRQIADDPINYSVLNIVVEVISAYGNVGFSTGYSC 504

BLAST of Cp4.1LG18g06440 vs. NCBI nr
Match: XP_023516517.1 (sodium transporter HKT1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 939 bits (2428), Expect = 0.0
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0

Query: 3   MKNLPLYFLRSPPSLRLPVSAANSFLAHLSYFITLSLIGYGSLKLTKTRTSATPNDLNIF 62
           MKNLPLYFLRSPPSLRLPVSAANSFLAHLSYFITLSLIGYGSLKLTKTRTSATPNDLNIF
Sbjct: 1   MKNLPLYFLRSPPSLRLPVSAANSFLAHLSYFITLSLIGYGSLKLTKTRTSATPNDLNIF 60

Query: 63  FTSVSAATVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFLSAVSFQLSHRNKQTNHDIPR 122
           FTSVSAATVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFLSAVSFQLSHRNKQTNHDIPR
Sbjct: 61  FTSVSAATVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFLSAVSFQLSHRNKQTNHDIPR 120

Query: 123 TAEFENESATVDELSISKSSFKVLGRIIIGYLLATNIGGACMIIIYLIMVPHARHVLEIK 182
           TAEFENESATVDELSISKSSFKVLGRIIIGYLLATNIGGACMIIIYLIMVPHARHVLEIK
Sbjct: 121 TAEFENESATVDELSISKSSFKVLGRIIIGYLLATNIGGACMIIIYLIMVPHARHVLEIK 180

Query: 183 GINFITFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGCLYPVGLRLVI 242
           GINFITFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGCLYPVGLRLVI
Sbjct: 181 GINFITFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGCLYPVGLRLVI 240

Query: 243 MAAAKVTGKKGWRYILKNDSAMGYSHLLSGVRCRFLAATAAGFIILQLIIFCSLEWNNSD 302
           MAAAKVTGKKGWRYILKNDSAMGYSHLLSGVRCRFLAATAAGFIILQLIIFCSLEWNNSD
Sbjct: 241 MAAAKVTGKKGWRYILKNDSAMGYSHLLSGVRCRFLAATAAGFIILQLIIFCSLEWNNSD 300

Query: 303 GIWDGMNPYEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLPPYTTFLPLRT 362
           GIWDGMNPYEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLPPYTTFLPLRT
Sbjct: 301 GIWDGMNPYEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLPPYTTFLPLRT 360

Query: 363 KEKASITTDGNDKRQHLVKFLTFSQLSYLAIFITLICISERDKLRNDPLNFTVLNIAVEV 422
           KEKASITTDGNDKRQHLVKFLTFSQLSYLAIFITLICISERDKLRNDPLNFTVLNIAVEV
Sbjct: 361 KEKASITTDGNDKRQHLVKFLTFSQLSYLAIFITLICISERDKLRNDPLNFTVLNIAVEV 420

Query: 423 ISAYGNVGFSSGYSCKRQVVADSTCRDAWYGFAGRWSAEGKLILILVMFFGRLKSFSMHG 482
           ISAYGNVGFSSGYSCKRQVVADSTCRDAWYGFAGRWSAEGKLILILVMFFGRLKSFSMHG
Sbjct: 421 ISAYGNVGFSSGYSCKRQVVADSTCRDAWYGFAGRWSAEGKLILILVMFFGRLKSFSMHG 480

Query: 483 GKAWKLS 489
           GKAWKLS
Sbjct: 481 GKAWKLS 487

BLAST of Cp4.1LG18g06440 vs. NCBI nr
Match: KAG7023349.1 (Sodium transporter HKT1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 909 bits (2350), Expect = 0.0
Identity = 471/487 (96.71%), Postives = 479/487 (98.36%), Query Frame = 0

Query: 3   MKNLPLYFLRSPPSLRLPVSAANSFLAHLSYFITLSLIGYGSLKLTKTRTSATPNDLNIF 62
           MKNLPLYFLRSPPSLRLPVSAANSFLAHLSYFITLSLIGYGSLKLTKTRTSA PNDLNIF
Sbjct: 1   MKNLPLYFLRSPPSLRLPVSAANSFLAHLSYFITLSLIGYGSLKLTKTRTSAAPNDLNIF 60

Query: 63  FTSVSAATVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFLSAVSFQLSHRNKQTNHDIPR 122
           FTSVSAATVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFLSAVSFQL+H N+QTNHDIP 
Sbjct: 61  FTSVSAATVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFLSAVSFQLAHHNQQTNHDIPH 120

Query: 123 TAEFENESATVDELSISKSSFKVLGRIIIGYLLATNIGGACMIIIYLIMVPHARHVLEIK 182
           T EFENESATVDELSISKSSFKVLGRIIIGYLLATNIGGACMIIIYLIMVPHARHVLEIK
Sbjct: 121 TVEFENESATVDELSISKSSFKVLGRIIIGYLLATNIGGACMIIIYLIMVPHARHVLEIK 180

Query: 183 GINFITFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGCLYPVGLRLVI 242
           GINFITFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGCLYPVGLRLVI
Sbjct: 181 GINFITFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGCLYPVGLRLVI 240

Query: 243 MAAAKVTGKKGWRYILKNDSAMGYSHLLSGVRCRFLAATAAGFIILQLIIFCSLEWNNSD 302
           MAAAKVTGKKGWRYILKNDSAMGYSHLLSGVRCRFLAATAAGFIILQ IIFCSLEWNNSD
Sbjct: 241 MAAAKVTGKKGWRYILKNDSAMGYSHLLSGVRCRFLAATAAGFIILQFIIFCSLEWNNSD 300

Query: 303 GIWDGMNPYEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLPPYTTFLPLRT 362
           GIWDGMNPYEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLPPYTTFLPLRT
Sbjct: 301 GIWDGMNPYEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLPPYTTFLPLRT 360

Query: 363 KEKASITTDGNDKRQHLVKFLTFSQLSYLAIFITLICISERDKLRNDPLNFTVLNIAVEV 422
           KEKASI  DGNDKRQHLVK+LTFSQLSYLAIFITLICI+ERDKL+NDPLNF+VLNIAVEV
Sbjct: 361 KEKASIA-DGNDKRQHLVKYLTFSQLSYLAIFITLICITERDKLKNDPLNFSVLNIAVEV 420

Query: 423 ISAYGNVGFSSGYSCKRQVVADSTCRDAWYGFAGRWSAEGKLILILVMFFGRLKSFSMHG 482
           ISAYGNVGFS+GYSCKRQVVADSTCRDAWYGFAGRWSA+GKLILILVMFFGRLKSFS HG
Sbjct: 421 ISAYGNVGFSTGYSCKRQVVADSTCRDAWYGFAGRWSAKGKLILILVMFFGRLKSFSRHG 480

Query: 483 GKAWKLS 489
           GKAWKLS
Sbjct: 481 GKAWKLS 486

BLAST of Cp4.1LG18g06440 vs. NCBI nr
Match: KAG6589668.1 (Sodium transporter HKT1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 902 bits (2331), Expect = 0.0
Identity = 467/487 (95.89%), Postives = 477/487 (97.95%), Query Frame = 0

Query: 3   MKNLPLYFLRSPPSLRLPVSAANSFLAHLSYFITLSLIGYGSLKLTKTRTSATPNDLNIF 62
           MKNLPLYFLRSPPSLRLPVSAANSFLAHLSYFITLSLIGYGSLKLTKTRTSA PNDLNIF
Sbjct: 1   MKNLPLYFLRSPPSLRLPVSAANSFLAHLSYFITLSLIGYGSLKLTKTRTSAAPNDLNIF 60

Query: 63  FTSVSAATVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFLSAVSFQLSHRNKQTNHDIPR 122
           FTSVSAATVSSMSVV+MEVFSNFQLLIITTLMFLGGEVFLSAVSFQL+H N+QTNHDIP 
Sbjct: 61  FTSVSAATVSSMSVVKMEVFSNFQLLIITTLMFLGGEVFLSAVSFQLAHHNQQTNHDIPH 120

Query: 123 TAEFENESATVDELSISKSSFKVLGRIIIGYLLATNIGGACMIIIYLIMVPHARHVLEIK 182
           T EFENESATVDEL+ISKSSFKVLGRIIIGYLLATNIGGACMIIIYLIMVPHARHVLEIK
Sbjct: 121 TVEFENESATVDELTISKSSFKVLGRIIIGYLLATNIGGACMIIIYLIMVPHARHVLEIK 180

Query: 183 GINFITFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGCLYPVGLRLVI 242
           GINFITFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGCLYPVGLRLVI
Sbjct: 181 GINFITFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGCLYPVGLRLVI 240

Query: 243 MAAAKVTGKKGWRYILKNDSAMGYSHLLSGVRCRFLAATAAGFIILQLIIFCSLEWNNSD 302
           MAAAKVTGKKGWRYILKNDSAMGYSHLLSGVRC FLAATA GFIILQ IIFCSLEWNNSD
Sbjct: 241 MAAAKVTGKKGWRYILKNDSAMGYSHLLSGVRCWFLAATAVGFIILQFIIFCSLEWNNSD 300

Query: 303 GIWDGMNPYEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLPPYTTFLPLRT 362
           GIWDGMNPYEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLPPYTTFLPLRT
Sbjct: 301 GIWDGMNPYEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLPPYTTFLPLRT 360

Query: 363 KEKASITTDGNDKRQHLVKFLTFSQLSYLAIFITLICISERDKLRNDPLNFTVLNIAVEV 422
           KEKASI  DGNDKRQHLVK+LTFSQLSYLAIFITLICI+ERDKL+NDPLNF+VLNIAVEV
Sbjct: 361 KEKASIA-DGNDKRQHLVKYLTFSQLSYLAIFITLICITERDKLKNDPLNFSVLNIAVEV 420

Query: 423 ISAYGNVGFSSGYSCKRQVVADSTCRDAWYGFAGRWSAEGKLILILVMFFGRLKSFSMHG 482
           ISAYGNVGFS+GYSCKRQVVADSTCRDAWYGFAGRWSA+GKLILILVMFFGRLKSFS HG
Sbjct: 421 ISAYGNVGFSTGYSCKRQVVADSTCRDAWYGFAGRWSAKGKLILILVMFFGRLKSFSRHG 480

Query: 483 GKAWKLS 489
           GKAWKLS
Sbjct: 481 GKAWKLS 486

BLAST of Cp4.1LG18g06440 vs. NCBI nr
Match: XP_022921350.1 (sodium transporter HKT1 [Cucurbita moschata])

HSP 1 Score: 897 bits (2317), Expect = 0.0
Identity = 465/487 (95.48%), Postives = 473/487 (97.13%), Query Frame = 0

Query: 3   MKNLPLYFLRSPPSLRLPVSAANSFLAHLSYFITLSLIGYGSLKLTKTRTSATPNDLNIF 62
           MKNLPLYFLRSPPSLRLPVSAANSFLAHL YFITLSLIGYGSLKLTKTRTSA PNDLNIF
Sbjct: 1   MKNLPLYFLRSPPSLRLPVSAANSFLAHLCYFITLSLIGYGSLKLTKTRTSAAPNDLNIF 60

Query: 63  FTSVSAATVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFLSAVSFQLSHRNKQTNHDIPR 122
           FTSVSAATVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFLSAVSFQLSH+NKQTNHD PR
Sbjct: 61  FTSVSAATVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFLSAVSFQLSHQNKQTNHDDPR 120

Query: 123 TAEFENESATVDELSISKSSFKVLGRIIIGYLLATNIGGACMIIIYLIMVPHARHVLEIK 182
           T EFENESATVDELSISKSSF VLGRIIIGYLLATNIGGACMIIIYLIMVPHARHVLE K
Sbjct: 121 TIEFENESATVDELSISKSSFMVLGRIIIGYLLATNIGGACMIIIYLIMVPHARHVLETK 180

Query: 183 GINFITFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGCLYPVGLRLVI 242
           GINF+TFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGCLYPVGLRLVI
Sbjct: 181 GINFVTFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGCLYPVGLRLVI 240

Query: 243 MAAAKVTGKKGWRYILKNDSAMGYSHLLSGVRCRFLAATAAGFIILQLIIFCSLEWNNSD 302
           MAAAKVTGKKGWRYILKN+S MGYSHLLSGVRCRFLAATAAGF+ILQ IIFCSLEWNNS 
Sbjct: 241 MAAAKVTGKKGWRYILKNNSVMGYSHLLSGVRCRFLAATAAGFVILQFIIFCSLEWNNSG 300

Query: 303 GIWDGMNPYEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLPPYTTFLPLRT 362
           GIWDGMNPYEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLPPYTTFLPLRT
Sbjct: 301 GIWDGMNPYEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLPPYTTFLPLRT 360

Query: 363 KEKASITTDGNDKRQHLVKFLTFSQLSYLAIFITLICISERDKLRNDPLNFTVLNIAVEV 422
           KEKASI  DGNDKRQH VK+ TFSQLSYLAIFITLICISERDKL+NDPLNF+VLNIAVEV
Sbjct: 361 KEKASIV-DGNDKRQHSVKYFTFSQLSYLAIFITLICISERDKLKNDPLNFSVLNIAVEV 420

Query: 423 ISAYGNVGFSSGYSCKRQVVADSTCRDAWYGFAGRWSAEGKLILILVMFFGRLKSFSMHG 482
           ISAYGNVGFSSGYSCKRQVVADSTCRDAWYGFAGRWSA+GKLILILVMFFGRLKSFS HG
Sbjct: 421 ISAYGNVGFSSGYSCKRQVVADSTCRDAWYGFAGRWSAKGKLILILVMFFGRLKSFSRHG 480

Query: 483 GKAWKLS 489
           GKAWKLS
Sbjct: 481 GKAWKLS 486

BLAST of Cp4.1LG18g06440 vs. NCBI nr
Match: XP_022988490.1 (sodium transporter HKT1 [Cucurbita maxima])

HSP 1 Score: 897 bits (2317), Expect = 0.0
Identity = 464/487 (95.28%), Postives = 476/487 (97.74%), Query Frame = 0

Query: 3   MKNLPLYFLRSPPSLRLPVSAANSFLAHLSYFITLSLIGYGSLKLTKTRTSATPNDLNIF 62
           MKNLPLYFLRSPPSL LPVSAANSFLAHLSYFITLSLIGYGSLKLTKTRTSA PNDLNIF
Sbjct: 1   MKNLPLYFLRSPPSLLLPVSAANSFLAHLSYFITLSLIGYGSLKLTKTRTSAAPNDLNIF 60

Query: 63  FTSVSAATVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFLSAVSFQLSHRNKQTNHDIPR 122
           FTSVSAATVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFLSAVSFQLSHRN+QTNHDIP 
Sbjct: 61  FTSVSAATVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFLSAVSFQLSHRNQQTNHDIPH 120

Query: 123 TAEFENESATVDELSISKSSFKVLGRIIIGYLLATNIGGACMIIIYLIMVPHARHVLEIK 182
           T E ENESATVDELSISKSSFKVLG+IIIGYLLATNIGGACMIIIYLIMVPHARHVLE K
Sbjct: 121 TVELENESATVDELSISKSSFKVLGQIIIGYLLATNIGGACMIIIYLIMVPHARHVLESK 180

Query: 183 GINFITFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGCLYPVGLRLVI 242
           GINF+TFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLL+ILAAEVLLGGCLYPVGLRLVI
Sbjct: 181 GINFVTFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLLILAAEVLLGGCLYPVGLRLVI 240

Query: 243 MAAAKVTGKKGWRYILKNDSAMGYSHLLSGVRCRFLAATAAGFIILQLIIFCSLEWNNSD 302
           M AAKVTGKKGWRYILKNDSAMGYSHLLSGVRC FLAATAAGFIILQ IIFCSLEWNNSD
Sbjct: 241 MVAAKVTGKKGWRYILKNDSAMGYSHLLSGVRCGFLAATAAGFIILQFIIFCSLEWNNSD 300

Query: 303 GIWDGMNPYEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLPPYTTFLPLRT 362
           GIWDGMNPYEKVVGSFFQVINSRHTGESV+DISLISPAILVVFLVMMYLPPYTTFLPLRT
Sbjct: 301 GIWDGMNPYEKVVGSFFQVINSRHTGESVMDISLISPAILVVFLVMMYLPPYTTFLPLRT 360

Query: 363 KEKASITTDGNDKRQHLVKFLTFSQLSYLAIFITLICISERDKLRNDPLNFTVLNIAVEV 422
           KEKASI  +GNDKRQHLVKFLTFSQLSYLAIFITLICI+ERDKL+NDPLNF+VLNIAVEV
Sbjct: 361 KEKASIA-NGNDKRQHLVKFLTFSQLSYLAIFITLICITERDKLKNDPLNFSVLNIAVEV 420

Query: 423 ISAYGNVGFSSGYSCKRQVVADSTCRDAWYGFAGRWSAEGKLILILVMFFGRLKSFSMHG 482
           ISAYGNVGFSSGYSCKRQVVADS+CR+AWYGFAGRWSA+GKLILILVMFFGRLKSFSMHG
Sbjct: 421 ISAYGNVGFSSGYSCKRQVVADSSCREAWYGFAGRWSAKGKLILILVMFFGRLKSFSMHG 480

Query: 483 GKAWKLS 489
           GKAWKLS
Sbjct: 481 GKAWKLS 486

BLAST of Cp4.1LG18g06440 vs. ExPASy TrEMBL
Match: A0A6J1JJP6 (sodium transporter HKT1 OS=Cucurbita maxima OX=3661 GN=LOC111485717 PE=3 SV=1)

HSP 1 Score: 897 bits (2317), Expect = 0.0
Identity = 464/487 (95.28%), Postives = 476/487 (97.74%), Query Frame = 0

Query: 3   MKNLPLYFLRSPPSLRLPVSAANSFLAHLSYFITLSLIGYGSLKLTKTRTSATPNDLNIF 62
           MKNLPLYFLRSPPSL LPVSAANSFLAHLSYFITLSLIGYGSLKLTKTRTSA PNDLNIF
Sbjct: 1   MKNLPLYFLRSPPSLLLPVSAANSFLAHLSYFITLSLIGYGSLKLTKTRTSAAPNDLNIF 60

Query: 63  FTSVSAATVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFLSAVSFQLSHRNKQTNHDIPR 122
           FTSVSAATVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFLSAVSFQLSHRN+QTNHDIP 
Sbjct: 61  FTSVSAATVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFLSAVSFQLSHRNQQTNHDIPH 120

Query: 123 TAEFENESATVDELSISKSSFKVLGRIIIGYLLATNIGGACMIIIYLIMVPHARHVLEIK 182
           T E ENESATVDELSISKSSFKVLG+IIIGYLLATNIGGACMIIIYLIMVPHARHVLE K
Sbjct: 121 TVELENESATVDELSISKSSFKVLGQIIIGYLLATNIGGACMIIIYLIMVPHARHVLESK 180

Query: 183 GINFITFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGCLYPVGLRLVI 242
           GINF+TFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLL+ILAAEVLLGGCLYPVGLRLVI
Sbjct: 181 GINFVTFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLLILAAEVLLGGCLYPVGLRLVI 240

Query: 243 MAAAKVTGKKGWRYILKNDSAMGYSHLLSGVRCRFLAATAAGFIILQLIIFCSLEWNNSD 302
           M AAKVTGKKGWRYILKNDSAMGYSHLLSGVRC FLAATAAGFIILQ IIFCSLEWNNSD
Sbjct: 241 MVAAKVTGKKGWRYILKNDSAMGYSHLLSGVRCGFLAATAAGFIILQFIIFCSLEWNNSD 300

Query: 303 GIWDGMNPYEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLPPYTTFLPLRT 362
           GIWDGMNPYEKVVGSFFQVINSRHTGESV+DISLISPAILVVFLVMMYLPPYTTFLPLRT
Sbjct: 301 GIWDGMNPYEKVVGSFFQVINSRHTGESVMDISLISPAILVVFLVMMYLPPYTTFLPLRT 360

Query: 363 KEKASITTDGNDKRQHLVKFLTFSQLSYLAIFITLICISERDKLRNDPLNFTVLNIAVEV 422
           KEKASI  +GNDKRQHLVKFLTFSQLSYLAIFITLICI+ERDKL+NDPLNF+VLNIAVEV
Sbjct: 361 KEKASIA-NGNDKRQHLVKFLTFSQLSYLAIFITLICITERDKLKNDPLNFSVLNIAVEV 420

Query: 423 ISAYGNVGFSSGYSCKRQVVADSTCRDAWYGFAGRWSAEGKLILILVMFFGRLKSFSMHG 482
           ISAYGNVGFSSGYSCKRQVVADS+CR+AWYGFAGRWSA+GKLILILVMFFGRLKSFSMHG
Sbjct: 421 ISAYGNVGFSSGYSCKRQVVADSSCREAWYGFAGRWSAKGKLILILVMFFGRLKSFSMHG 480

Query: 483 GKAWKLS 489
           GKAWKLS
Sbjct: 481 GKAWKLS 486

BLAST of Cp4.1LG18g06440 vs. ExPASy TrEMBL
Match: A0A6J1E5E2 (sodium transporter HKT1 OS=Cucurbita moschata OX=3662 GN=LOC111429647 PE=3 SV=1)

HSP 1 Score: 897 bits (2317), Expect = 0.0
Identity = 465/487 (95.48%), Postives = 473/487 (97.13%), Query Frame = 0

Query: 3   MKNLPLYFLRSPPSLRLPVSAANSFLAHLSYFITLSLIGYGSLKLTKTRTSATPNDLNIF 62
           MKNLPLYFLRSPPSLRLPVSAANSFLAHL YFITLSLIGYGSLKLTKTRTSA PNDLNIF
Sbjct: 1   MKNLPLYFLRSPPSLRLPVSAANSFLAHLCYFITLSLIGYGSLKLTKTRTSAAPNDLNIF 60

Query: 63  FTSVSAATVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFLSAVSFQLSHRNKQTNHDIPR 122
           FTSVSAATVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFLSAVSFQLSH+NKQTNHD PR
Sbjct: 61  FTSVSAATVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFLSAVSFQLSHQNKQTNHDDPR 120

Query: 123 TAEFENESATVDELSISKSSFKVLGRIIIGYLLATNIGGACMIIIYLIMVPHARHVLEIK 182
           T EFENESATVDELSISKSSF VLGRIIIGYLLATNIGGACMIIIYLIMVPHARHVLE K
Sbjct: 121 TIEFENESATVDELSISKSSFMVLGRIIIGYLLATNIGGACMIIIYLIMVPHARHVLETK 180

Query: 183 GINFITFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGCLYPVGLRLVI 242
           GINF+TFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGCLYPVGLRLVI
Sbjct: 181 GINFVTFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGCLYPVGLRLVI 240

Query: 243 MAAAKVTGKKGWRYILKNDSAMGYSHLLSGVRCRFLAATAAGFIILQLIIFCSLEWNNSD 302
           MAAAKVTGKKGWRYILKN+S MGYSHLLSGVRCRFLAATAAGF+ILQ IIFCSLEWNNS 
Sbjct: 241 MAAAKVTGKKGWRYILKNNSVMGYSHLLSGVRCRFLAATAAGFVILQFIIFCSLEWNNSG 300

Query: 303 GIWDGMNPYEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLPPYTTFLPLRT 362
           GIWDGMNPYEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLPPYTTFLPLRT
Sbjct: 301 GIWDGMNPYEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLPPYTTFLPLRT 360

Query: 363 KEKASITTDGNDKRQHLVKFLTFSQLSYLAIFITLICISERDKLRNDPLNFTVLNIAVEV 422
           KEKASI  DGNDKRQH VK+ TFSQLSYLAIFITLICISERDKL+NDPLNF+VLNIAVEV
Sbjct: 361 KEKASIV-DGNDKRQHSVKYFTFSQLSYLAIFITLICISERDKLKNDPLNFSVLNIAVEV 420

Query: 423 ISAYGNVGFSSGYSCKRQVVADSTCRDAWYGFAGRWSAEGKLILILVMFFGRLKSFSMHG 482
           ISAYGNVGFSSGYSCKRQVVADSTCRDAWYGFAGRWSA+GKLILILVMFFGRLKSFS HG
Sbjct: 421 ISAYGNVGFSSGYSCKRQVVADSTCRDAWYGFAGRWSAKGKLILILVMFFGRLKSFSRHG 480

Query: 483 GKAWKLS 489
           GKAWKLS
Sbjct: 481 GKAWKLS 486

BLAST of Cp4.1LG18g06440 vs. ExPASy TrEMBL
Match: A0A0A0M019 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G434130 PE=3 SV=1)

HSP 1 Score: 733 bits (1891), Expect = 1.19e-262
Identity = 375/498 (75.30%), Postives = 429/498 (86.14%), Query Frame = 0

Query: 3   MKNLPLYFLRSPPSLRLPVSAANSFLAHLSYFITLSLIGYGSLKLTKTRTSATPNDLNIF 62
           MKN+PLYFLRSPPSLRLP+  AN FL+HLSYFIT+SL G+G+LKLT+TRTS++PNDLN+F
Sbjct: 1   MKNIPLYFLRSPPSLRLPLPPANPFLSHLSYFITISLFGFGALKLTQTRTSSSPNDLNLF 60

Query: 63  FTSVSAATVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFLSAVSFQLSHR----NKQTNH 122
           FTSVSA +VSSMSVVEMEVFSNFQLLIITTLMFLGGEVF+SAV+FQLS R    N++ + 
Sbjct: 61  FTSVSAVSVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFVSAVAFQLSRRFKYLNEENHV 120

Query: 123 DIPRTAEFENESA------TVDELSISKSSFKVLGRIIIGYLLATNIGGACMIIIYLIMV 182
           D  R+ E E          T+DELSIS+S  KVLG  IIGYLLATN+ G+ +I IY+I+V
Sbjct: 121 DSVRSIEMEENGTSSSTMVTIDELSISRSCSKVLGHTIIGYLLATNVLGSFLIFIYIIVV 180

Query: 183 PHARHVLEIKGINFITFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGC 242
           PHA+ VLE KGINF TFSIFSTVSTFTNCGFIPTNENMI F  NSGLL+IL  +VLLGGC
Sbjct: 181 PHAKQVLESKGINFFTFSIFSTVSTFTNCGFIPTNENMIAFNNNSGLLLILIPQVLLGGC 240

Query: 243 LYPVGLRLVIMAAAKVTGKKGWRYILKNDSAMGYSHLLSGVRCRFLAATAAGFIILQLII 302
           LYPVGLRLVIMA AKV+GKK WRYILKN+S MGYSHLL G+ C FLA T AGFI+ QLII
Sbjct: 241 LYPVGLRLVIMAVAKVSGKKEWRYILKNESKMGYSHLLPGLHCGFLAVTVAGFIVFQLII 300

Query: 303 FCSLEWNNSDGIWDGMNPYEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLP 362
           FCSLEWNNSDGIWDG+NPY+K V S FQV NSRHTGES+VDIS+IS AILVVF+VMMYLP
Sbjct: 301 FCSLEWNNSDGIWDGLNPYQKFVASLFQVTNSRHTGESIVDISVISQAILVVFVVMMYLP 360

Query: 363 PYTTFLPLRT-KEKASITTDGNDKRQHLVKFLTFSQLSYLAIFITLICISERDKLRNDPL 422
           PYTTFLP+R  KE+ASI TDG ++RQ+LV+  TFSQLSYLAIFI LICI+E+ KLR+DPL
Sbjct: 361 PYTTFLPIRNNKERASIMTDGKNRRQYLVELFTFSQLSYLAIFIILICITEKQKLRDDPL 420

Query: 423 NFTVLNIAVEVISAYGNVGFSSGYSCKRQVVADSTCRDAWYGFAGRWSAEGKLILILVMF 482
           NFT+LNI +EVISAYGNVGFSSGYSCKRQ+  DS+C+DAWYGFAGRWS++GK ILILVM 
Sbjct: 421 NFTLLNITIEVISAYGNVGFSSGYSCKRQIKVDSSCKDAWYGFAGRWSSKGKFILILVMI 480

Query: 483 FGRLKSFSMHGGKAWKLS 489
           FGR+KSF+MH GKAWKLS
Sbjct: 481 FGRMKSFTMHTGKAWKLS 498

BLAST of Cp4.1LG18g06440 vs. ExPASy TrEMBL
Match: A0A1S3BWJ7 (sodium transporter HKT1-like OS=Cucumis melo OX=3656 GN=LOC103494214 PE=3 SV=1)

HSP 1 Score: 726 bits (1873), Expect = 6.07e-260
Identity = 370/496 (74.60%), Postives = 421/496 (84.88%), Query Frame = 0

Query: 3   MKNLPLYFLRSPPSLRLPVSAANSFLAHLSYFITLSLIGYGSLKLTKTRTSATPNDLNIF 62
           MKNLPLYFLRSPPSLRLP+ AAN FL+HLSYFIT+SL G+G+LKLT TRTS++P+DLN+F
Sbjct: 1   MKNLPLYFLRSPPSLRLPLPAANHFLSHLSYFITISLFGFGALKLTNTRTSSSPSDLNLF 60

Query: 63  FTSVSAATVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFLSAVSFQLSHRNKQTN--HDI 122
           FTSVSA +VSSMSVVEMEVFSNFQLLIITTLMFLGGEVF+SAV FQLS R K  N  + +
Sbjct: 61  FTSVSAVSVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFVSAVGFQLSRRFKYLNEKNHV 120

Query: 123 PRTAEFENESAT-------VDELSISKSSFKVLGRIIIGYLLATNIGGACMIIIYLIMVP 182
                 E  S T       +DEL ISKS  K+LG  IIGYLL TNI G+  I +Y+++VP
Sbjct: 121 GSVRSIEMGSGTSSSTMVTIDELGISKSCSKLLGHTIIGYLLGTNILGSFFIFVYILVVP 180

Query: 183 HARHVLEIKGINFITFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGCL 242
           HA+ VLE KGIN++TFSIFST+STFTNCGFIPTNENMI F  NSGLL+IL  +VLLGGCL
Sbjct: 181 HAKQVLESKGINYLTFSIFSTISTFTNCGFIPTNENMIAFNNNSGLLLILIPQVLLGGCL 240

Query: 243 YPVGLRLVIMAAAKVTGKKGWRYILKNDSAMGYSHLLSGVRCRFLAATAAGFIILQLIIF 302
           YPVGLRLVIMAAAKVTGKK WRYILKNDS MGYSHLLSG+RC FL  T AGFI+ QLIIF
Sbjct: 241 YPVGLRLVIMAAAKVTGKKEWRYILKNDSEMGYSHLLSGLRCGFLVVTVAGFIVFQLIIF 300

Query: 303 CSLEWNNSDGIWDGMNPYEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLPP 362
           CSLEWNNS GIWDG+NPY+K V S FQVINSRHTGESVVDIS+IS AILV+F+VMMYLPP
Sbjct: 301 CSLEWNNSGGIWDGLNPYQKFVASLFQVINSRHTGESVVDISVISQAILVIFVVMMYLPP 360

Query: 363 YTTFLPLRTKEKASITTDGNDKRQHLVKFLTFSQLSYLAIFITLICISERDKLRNDPLNF 422
           YTTFLP+R  ++ +  TDG D+RQ LV+  TFSQLSYLAIFI L+CI+E+ KLR+DPLNF
Sbjct: 361 YTTFLPIRNYKETASMTDGKDRRQQLVELFTFSQLSYLAIFIILLCITEKQKLRDDPLNF 420

Query: 423 TVLNIAVEVISAYGNVGFSSGYSCKRQVVADSTCRDAWYGFAGRWSAEGKLILILVMFFG 482
           TVLNI +EVISAYGNVGFSSGYSCKRQ+  DS+C+DAWYGFAGRWS++GK ILILVM FG
Sbjct: 421 TVLNITIEVISAYGNVGFSSGYSCKRQIKVDSSCKDAWYGFAGRWSSKGKFILILVMIFG 480

Query: 483 RLKSFSMHGGKAWKLS 489
           R+KSF+MH GKAWKLS
Sbjct: 481 RMKSFTMHSGKAWKLS 496

BLAST of Cp4.1LG18g06440 vs. ExPASy TrEMBL
Match: A0A6J1C0X2 (sodium transporter HKT1 OS=Momordica charantia OX=3673 GN=LOC111007262 PE=3 SV=1)

HSP 1 Score: 630 bits (1626), Expect = 1.90e-222
Identity = 340/497 (68.41%), Postives = 399/497 (80.28%), Query Frame = 0

Query: 2   EMKNLPLYFLRSPPSLRLPVSAANSFLAHLSYFITLSLIGYGSLKLTKTRTSATPNDLNI 61
            M NL L  LRS P L   ++AAN F   LS+F+TLSL+G+G+LK+T+TRTS+ PN LN 
Sbjct: 2   SMLNL-LDLLRSQPLL---MAAANPFWPALSHFLTLSLLGFGALKVTRTRTSSPPNHLNF 61

Query: 62  FFTSVSAATVSSMSVVEMEVFSNFQLLIITTLMFLGGEVFLSAVSFQLSHRNKQT--NHD 121
           FFT+VSAATVSSMS VEMEVFSN QLLI+T LMFLGGEVF+SA +F LS R   +  N  
Sbjct: 62  FFTAVSAATVSSMSTVEMEVFSNAQLLIVTLLMFLGGEVFVSAAAFHLSRRFSSSVKNPT 121

Query: 122 IP-RTAEFENE-----SATV-DELSISKSSFKVLGRIIIGYLLATNIGGACMIIIYLIMV 181
            P R+ + E E     S TV DE    + S  +LGR+++GYLLA N+ G+  + +Y+ +V
Sbjct: 122 GPARSIDSELEIGHGRSMTVTDEFVNHEKSTALLGRVVVGYLLAANLIGSLFVFLYVTLV 181

Query: 182 PHARHVLEIKGINFITFSIFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGC 241
             ARHVLE KGIN +TFSIF+TVSTFTNCGFIPTNENMIVFK +SG+L+ L A+VLLGGC
Sbjct: 182 SRARHVLESKGINLVTFSIFTTVSTFTNCGFIPTNENMIVFKDSSGILLALVAQVLLGGC 241

Query: 242 LYPVGLRLVIMAAAKVTGKKGWRYILKNDSAMGYSHLLSGVRCRFLAATAAGFIILQLII 301
           LYP GLRLVI    KVTGKK  RYIL N S MGY HLLSGVRCR+LA TAAGF+ILQ+II
Sbjct: 242 LYPAGLRLVISGMTKVTGKKELRYILNNYSEMGYPHLLSGVRCRYLAGTAAGFVILQMII 301

Query: 302 FCSLEWNNSDGIWDGMNPYEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLP 361
           FCSLEWN S+G+WDG+NPY K V + FQV NSRHTGESVVDIS+ISPAILV+F+VMMYLP
Sbjct: 302 FCSLEWN-SEGLWDGLNPYRKTVAALFQVTNSRHTGESVVDISVISPAILVLFVVMMYLP 361

Query: 362 PYTTFLPLRTKEKASITTDGNDKRQHLVKFLTFSQLSYLAIFITLICISERDKLRNDPLN 421
           PYTTFLP++ KE  S+  +G  KRQHLV FL FSQLSYLAIFI LICI+E+ +LR+DPLN
Sbjct: 362 PYTTFLPMKNKEMDSVG-NGKGKRQHLVGFLKFSQLSYLAIFIILICITEKQQLRDDPLN 421

Query: 422 FTVLNIAVEVISAYGNVGFSSGYSCKRQVVADSTCRDAWYGFAGRWSAEGKLILILVMFF 481
           FTVLNI +EVISAYGNVGFS+GYSCKRQ+ AD +C+DAWYGF GRWS +GK ILILVM F
Sbjct: 422 FTVLNITLEVISAYGNVGFSTGYSCKRQLKADGSCKDAWYGFVGRWSGKGKFILILVMIF 481

Query: 482 GRLKSFSMHGGKAWKLS 489
           GRLKSFSMHGG+AWKLS
Sbjct: 482 GRLKSFSMHGGRAWKLS 492

BLAST of Cp4.1LG18g06440 vs. TAIR 10
Match: AT4G10310.1 (high-affinity K+ transporter 1 )

HSP 1 Score: 439.5 bits (1129), Expect = 3.5e-123
Identity = 236/482 (48.96%), Postives = 330/482 (68.46%), Query Frame = 0

Query: 27  FLAHLSYFITLSLIGYGSLKLTKTRTSATPNDLNIFFTSVSAATVSSMSVVEMEVFSNFQ 86
           F  +  YF+  S +G+ +LK+TK RT++ P+D ++FFTSVSA TVSSMS V+MEVFSN Q
Sbjct: 22  FFLYFIYFLFFSFLGFLALKITKPRTTSRPHDFDLFFTSVSAITVSSMSTVDMEVFSNTQ 81

Query: 87  LLIITTLMFLGGEVFLSAVSFQLSHRNKQT--NHDIPRTAEFENESATVDE--------- 146
           L+ +T LMFLGGE+F S ++  +S+  K    ++ I       N  +++++         
Sbjct: 82  LIFLTILMFLGGEIFTSFLNLYVSYFTKFVFPHNKIRHILGSYNSDSSIEDRCDVETVTD 141

Query: 147 -----LSISKSSFKVLGRIIIGYLLATNIGGACMIIIYLIMVPHARHVLEIKGINFITFS 206
                + I + + K L  +++ Y L TN+ G+ ++++Y+  V  AR VL  K I+ +TFS
Sbjct: 142 YREGLIKIDERASKCLYSVVLSYHLVTNLVGSVLLLVYVNFVKTARDVLSSKEISPLTFS 201

Query: 207 IFSTVSTFTNCGFIPTNENMIVFKKNSGLLIILAAEVLLGGCLYPVGLRLVIMAAAKVTG 266
           +F+TVSTF NCGF+PTNENMI+F+KNSGL+ +L  +VL+G  L+P  L L+I    K+T 
Sbjct: 202 VFTTVSTFANCGFVPTNENMIIFRKNSGLIWLLIPQVLMGNTLFPCFLVLLIWGLYKITK 261

Query: 267 KKGWRYILKNDSAMGYSHLLSGVRCRFLAATAAGFIILQLIIFCSLEWNNSDGIWDGMNP 326
           +  + YILKN + MGYSHLLS   C  L  T  GF+I+QL+ FC+ EW +     +GM+ 
Sbjct: 262 RDEYGYILKNHNKMGYSHLLSVRLCVLLGVTVLGFLIIQLLFFCAFEWTSES--LEGMSS 321

Query: 327 YEKVVGSFFQVINSRHTGESVVDISLISPAILVVFLVMMYLPPYTTFLPL---RTKEKAS 386
           YEK+VGS FQV+NSRHTGE++VD+S +SPAILV+F++MMYLPPYT F+PL   +T EK  
Sbjct: 322 YEKLVGSLFQVVNSRHTGETIVDLSTLSPAILVLFILMMYLPPYTLFMPLTEQKTIEKEG 381

Query: 387 ITTDGNDKRQHLVKFLTFSQLSYLAIFITLICISERDKLRNDPLNFTVLNIAVEVISAYG 446
              D  + ++     L  SQLS+L I I LI I+ER  L+ DP+NF VLNI +EVISAYG
Sbjct: 382 GDDDSENGKKVKKSGLIVSQLSFLTICIFLISITERQNLQRDPINFNVLNITLEVISAYG 441

Query: 447 NVGFSSGYSCKRQV-VADSTCRDAWYGFAGRWSAEGKLILILVMFFGRLKSFSMHGGKAW 489
           NVGF++GYSC+R+V ++D  C+DA YGFAGRWS  GK +LI+VMF+GR K F+   G+AW
Sbjct: 442 NVGFTTGYSCERRVDISDGGCKDASYGFAGRWSPMGKFVLIIVMFYGRFKQFTAKSGRAW 501