Cp4.1LG18g05910 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG18g05910
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	tRNA (Adenine(37)-N6)-methyltransferase
Location	Cp4.1LG18: 6360556 .. 6363909 (+)
RNA-Seq Expression	Cp4.1LG18g05910
Synteny	Cp4.1LG18g05910

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAACTAAGTATAAAACCCCAAAAGCCGGGAATTGGTCAAGCCTCAAGCTGCCGGATCCAGTTCGCCTGAACGCTGAAGCTACTTCCCGACTCTGAAGTCGAAATGGCCTACGCCGCGGTATCAAGATCGTCGACGGCTGCCATGACCGTCACATTGGCGTTTGTATCCGCCTCCGCCATCCTATCTCGTAAAACTTCGATAACTTTTAAGATCCTTTATGTCCTCTCTCTCAATTTCTCCGATTTACTACGTGATTCTCACAAGTCGTGTTTTTGTGGGCTTCTCCAGTTTATTTCGTGACGAAGAAGTCGAGAGAGTTGGGTTCCAAAGTCCGAGAGCTTGAGGCTTCTCTGGCAGCTTCTTTAAAGAAAACCATCGCCGAGAGACAAGGCCGAATACGGGCACAGCAGGTGTTTCATATTTGCTCCTCTGCATCGCTTCATGTTCCTGACTCCAATGTTGTTATTGATATTTTGATTTTTTTTTTTTTTTTTCCTTCCTGTTTGTAGGCTTTAAGGAAAGCAGTTTCGGAACCGAAGTCTGATAGCGTGGAGTGTACTTTGTATCCCATGGCACCAATTGGTACTATCCAATCATCCTTCTCTACTAGGTTAGTTTTAGATTGCATATCTGGTAGTCTAAACATCAATTATTGGGTTGTTGTTTTCTCATAGTTCTGTTCACAAAACTAGTTACAGCCGTATTCTTCAGCTAGCGCTGTTGGTTATTTTTTTTTTTTTTTTAAGGAATGGGACTCCGAGGCAATCTTTAGTCGTACCTCTTGCGAGGGCAAGGTTGGTGTTTTCTTCTCGCATTCCTCCAGCGTCCATTGAAGGTCTTGGAGAATATTCCCATTGCTGGGTCATATATGTCTTTCATTTAAACACAAATCTTGACAAGTTGTGGAAGGATCCATCGAAATCAAAATTCAAGGCAAAAGTACGCTTCTTCATTTGTTGATGATCCTTAAATATGGAATTCACATTTCTATTATTCTTATAATTCCCTAAAATCCCATAAAAGGACAGCTCAATGGTAGGTTAAAGTACCGAGGTTGAAAGGCGAAAAAGTAGGAATCTTTGCTACGAGATCCCCACATAGACCATGCCCAATTGGAATCACCATTGCAAAGGTAAGATAACATTTTTGATTGCTGTCAATGTATCTTGCAAGTTGATGGAGAAGGCCATCATTCATCACATTGATGAAAATATGTCCCCCCCCCCCCAAATGAATTCTAGAGAGGTTTCACTGTCACATGAACTGGTCGAATGTGTTTTTGTATGGTTAGGCTGTTTCAGTTTCATTTTGCATTAGAAATTTATGGCTACAAGCTTACAGTTGAAATTGTTAAACAACATTAAAATGCAATAGATAAATGGGGGAATATTAACTCTAAGATTAAAAAATCTGCTGTACCACTTCAGGTGGAGGCTATCCAGGGACATCAAATTCTACTCTCTGGTGCCGATCTTGTTGATGGAACGGTAAGGGTTTGTTTCATTTTTTTCCACCAGCGATAAAGGTGTAATCTTGAATTTAGTCATTACTTCTTGTATTAATGATTGTTTTGATTTCTTGCTTTGTTCGAATTTTGTTACAGCCAGTACTTGATGTTAAACCTTATCTACCGTACTGTGACAGTATCCAAGATGCAACAGTCCCTAAGTGGTTAACGGTACTTCTTTCCTTGATGCTTATGTTACTATTCATCCTTTCAAATCATTCTTTGTGTGTATGTGTTGTGCTTTTCAACTTCAAAATGTGGACAATCTCTTCTCATTTGCGTGCCATATGTGATATAACTAGTTGCGAGCAAATAACTTAAGAAATTCAACTTTCCATATGTTATTTGGTGCTACTGCTACTTTCATTGTTGGAGGCATGATACTCTATAACCATGTAATATGTTTTTATACATTCTTCCTGGCAATCTAACATCTTATTGACGCAAACACAGGGGGAAAATGTATTGTCTGTTGCATCCATTTGCTTCTCTGAGGAATTTACTCCCTCTCTATCGGAGTGTTGGCCACTAATAGTGAGTTCCTTTTTTAAAAATTAATTAATTAATTATTACTGATGTTGTTTTTTTATATAACCACGAGTGTTTGGGCCAACTTGCGCACCTCAACAATATGCCAAAAATATTCACTATTTTTATTTTTATTTTTATTTTTTTTTTTTGCACGTGAGAGGAAAAAAAACAGTATGGTTTCTTTGGTTTCATTGGTATCAAACAAAAGGGACCTTTCAAGCTATTATATTTTATGCCTTAAAAGACGTTACTGGAGTTTTGGGTGATAATCAGCCTGAGGTTGGTGCTTTTGATGCAAAGCTTAATAAAAAAAAAGTGAAAACCCTTGATATCTCCTGGTGGTGTTGTAGCGCACAGAGTCTGAATTCCTTAAGAAAACTATGCCTCTGGAAATTTACATTATGAATATTGAGAGAGAGATAGCTGCTTTGTAGTCTGATTACCATTTTTATGAAGCTCCACCTTACAAAGAGCTAGTGCTCACAAGTGCTCATAATTTCTCATGTATCATAATTTTCTTGTTTCAGGAAAAGAAATCGCTTTATGCATCGGCAGATGAGTTGAAAAACTTTGTGAAGCAGGTGCTTTCATGGGATATTCGATCATTGTCACAACGTAGCCAACCTCATGAATCCTACCTTACGACTACAAGAGGACATGAGTTGTCCGATGCTAGTTCAGATTCAGATGGTCACCTAGATGAAACACTTCCTAGTGGAACTTCCCAAAGTTCTTCTAAGAGCGTTGTCTATCACCTACTTGTAGATGGATTAAATGTGTCGTACACAATCGGTTGCCAAGGCGACGTGGTTGTGAGAAAAGTGGAACTTGCATCACCCAGCTGAATAGGCAAATAAGGGTAACTTGTCTTTTTACTATGTAGGGAATTCTCACTCAAAACTAAACTGCCTTGTATGGAGCTCGATCTCCTTACATTTCTTATTCATCTTTCTCTTCACAAAAGGTGTATGGTTGAAATTGAGACAAGCATGCTGGATAGAATCTTAATTGTGAGTTGTTAGATGGTCAGTGAAATAGTTTTGGTTGAAAATGTGTGGCTATCTGCTGATGAGTGATTGTTTGACTTTTGTACCACATTCTTAAAGAAGAAAGGGAAATGTTTTGAAGTCTATACTCCAGAAGTATGAACTGTTACCACAAGTGACTAAGTCACATCGAGCTGAACTGATGCAGCTTGGATCAACTAAGATGAAAACAAAGGATCTTTTGGACGTGGTTCCAGTTCACATGAGGCATTAATCCATGGTTTGGTTTGGGCTTTGTTGGTTTTTATTTTTCATTTTTGAAGCATAT

mRNA sequence

AAGAACTAAGTATAAAACCCCAAAAGCCGGGAATTGGTCAAGCCTCAAGCTGCCGGATCCAGTTCGCCTGAACGCTGAAGCTACTTCCCGACTCTGAAGTCGAAATGGCCTACGCCGCGGTATCAAGATCGTCGACGGCTGCCATGACCGTCACATTGGCGTTTGTATCCGCCTCCGCCATCCTATCTCTTTATTTCGTGACGAAGAAGTCGAGAGAGTTGGGTTCCAAAGTCCGAGAGCTTGAGGCTTCTCTGGCAGCTTCTTTAAAGAAAACCATCGCCGAGAGACAAGGCCGAATACGGGCACAGCAGGCTTTAAGGAAAGCAGTTTCGGAACCGAAGTCTGATAGCGTGGAGTGTACTTTGTATCCCATGGCACCAATTGGTACTATCCAATCATCCTTCTCTACTAGGAATGGGACTCCGAGGCAATCTTTAGTCGTACCTCTTGCGAGGGCAAGGTTGGTGTTTTCTTCTCGCATTCCTCCAGCGTCCATTGAAGGTCTTGGAGAATATTCCCATTGCTGGGTCATATATGTCTTTCATTTAAACACAAATCTTGACAAGTTGTGGAAGGATCCATCGAAATCAAAATTCAAGGCAAAAGTTAAAGTACCGAGGTTGAAAGGCGAAAAAGTAGGAATCTTTGCTACGAGATCCCCACATAGACCATGCCCAATTGGAATCACCATTGCAAAGGTGGAGGCTATCCAGGGACATCAAATTCTACTCTCTGGTGCCGATCTTGTTGATGGAACGCCAGTACTTGATGTTAAACCTTATCTACCGTACTGTGACAGTATCCAAGATGCAACAGTCCCTAAGTGGTTAACGGGGGAAAATGTATTGTCTGTTGCATCCATTTGCTTCTCTGAGGAATTTACTCCCTCTCTATCGGAGTGTTGGCCACTAATAGAAAAGAAATCGCTTTATGCATCGGCAGATGAGTTGAAAAACTTTGTGAAGCAGGTGCTTTCATGGGATATTCGATCATTGTCACAACGTAGCCAACCTCATGAATCCTACCTTACGACTACAAGAGGACATGAGTTGTCCGATGCTAGTTCAGATTCAGATGGTCACCTAGATGAAACACTTCCTAGTGGAACTTCCCAAAGTTCTTCTAAGAGCGTTGTCTATCACCTACTTGTAGATGGATTAAATGTGTCGTACACAATCGGTTGCCAAGGCGACGTGGTTGTGAGAAAAGTGGAACTTGCATCACCCAGCTGAATAGGCAAATAAGGGTAACTTGTCTTTTTACTATGTAGGGAATTCTCACTCAAAACTAAACTGCCTTGTATGGAGCTCGATCTCCTTACATTTCTTATTCATCTTTCTCTTCACAAAAGGTGTATGGTTGAAATTGAGACAAGCATGCTGGATAGAATCTTAATTGTGAGTTGTTAGATGGTCAGTGAAATAGTTTTGGTTGAAAATGTGTGGCTATCTGCTGATGAGTGATTGTTTGACTTTTGTACCACATTCTTAAAGAAGAAAGGGAAATGTTTTGAAGTCTATACTCCAGAAGTATGAACTGTTACCACAAGTGACTAAGTCACATCGAGCTGAACTGATGCAGCTTGGATCAACTAAGATGAAAACAAAGGATCTTTTGGACGTGGTTCCAGTTCACATGAGGCATTAATCCATGGTTTGGTTTGGGCTTTGTTGGTTTTTATTTTTCATTTTTGAAGCATAT

Coding sequence (CDS)

ATGGCCTACGCCGCGGTATCAAGATCGTCGACGGCTGCCATGACCGTCACATTGGCGTTTGTATCCGCCTCCGCCATCCTATCTCTTTATTTCGTGACGAAGAAGTCGAGAGAGTTGGGTTCCAAAGTCCGAGAGCTTGAGGCTTCTCTGGCAGCTTCTTTAAAGAAAACCATCGCCGAGAGACAAGGCCGAATACGGGCACAGCAGGCTTTAAGGAAAGCAGTTTCGGAACCGAAGTCTGATAGCGTGGAGTGTACTTTGTATCCCATGGCACCAATTGGTACTATCCAATCATCCTTCTCTACTAGGAATGGGACTCCGAGGCAATCTTTAGTCGTACCTCTTGCGAGGGCAAGGTTGGTGTTTTCTTCTCGCATTCCTCCAGCGTCCATTGAAGGTCTTGGAGAATATTCCCATTGCTGGGTCATATATGTCTTTCATTTAAACACAAATCTTGACAAGTTGTGGAAGGATCCATCGAAATCAAAATTCAAGGCAAAAGTTAAAGTACCGAGGTTGAAAGGCGAAAAAGTAGGAATCTTTGCTACGAGATCCCCACATAGACCATGCCCAATTGGAATCACCATTGCAAAGGTGGAGGCTATCCAGGGACATCAAATTCTACTCTCTGGTGCCGATCTTGTTGATGGAACGCCAGTACTTGATGTTAAACCTTATCTACCGTACTGTGACAGTATCCAAGATGCAACAGTCCCTAAGTGGTTAACGGGGGAAAATGTATTGTCTGTTGCATCCATTTGCTTCTCTGAGGAATTTACTCCCTCTCTATCGGAGTGTTGGCCACTAATAGAAAAGAAATCGCTTTATGCATCGGCAGATGAGTTGAAAAACTTTGTGAAGCAGGTGCTTTCATGGGATATTCGATCATTGTCACAACGTAGCCAACCTCATGAATCCTACCTTACGACTACAAGAGGACATGAGTTGTCCGATGCTAGTTCAGATTCAGATGGTCACCTAGATGAAACACTTCCTAGTGGAACTTCCCAAAGTTCTTCTAAGAGCGTTGTCTATCACCTACTTGTAGATGGATTAAATGTGTCGTACACAATCGGTTGCCAAGGCGACGTGGTTGTGAGAAAAGTGGAACTTGCATCACCCAGCTGA

Protein sequence

MAYAAVSRSSTAAMTVTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAERQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARLVFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGIFATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPKWLTGENVLSVASICFSEEFTPSLSECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQRSQPHESYLTTTRGHELSDASSDSDGHLDETLPSGTSQSSSKSVVYHLLVDGLNVSYTIGCQGDVVVRKVELASPS

Homology

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BLAST of Cp4.1LG18g05910 vs. ExPASy Swiss-Prot
Match: Q9BU70 (tRNA (adenine(37)-N6)-methyltransferase OS=Homo sapiens OX=9606 GN=TRMO PE=1 SV=2)

HSP 1 Score: 142.1 bits (357), Expect = 1.3e-32
Identity = 71/151 (47.02%), Postives = 97/151 (64.24%), Query Frame = 0


Query: 92  PIGTIQSSFSTRNGTPRQSLVVPLARARLVFSSRI---PPASIEGLGEYSHCWVIYVFHL 151
           P+G ++S FS +NGTPRQ  +   +RA L    RI   P  S+ GL ++SH W+++VFH 
Sbjct: 32  PVGYLESCFSAKNGTPRQPSICSYSRACLRIRKRIFNNPEHSLMGLEQFSHVWILFVFHK 91

Query: 152 NTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGIFATRSPHRPCPIGITIAKVEAIQGHQIL 211
           N +L            KAKV+ PRL G K G+F+TRSPHRP  IG+T+AK+E ++G  I 
Sbjct: 92  NGHL----------SCKAKVQPPRLNGAKTGVFSTRSPHRPNAIGLTLAKLEKVEGGAIY 151

Query: 212 LSGADLVDGTPVLDVKPYLPYCDSIQDATVP 240
           LSG D++ GTPVLD+KPY+   DS Q+   P
Sbjct: 152 LSGIDMIHGTPVLDIKPYIAEYDSPQNVMEP 172

[Close]

BLAST of Cp4.1LG18g05910 vs. ExPASy Swiss-Prot
Match: Q562D6 (tRNA (adenine(37)-N6)-methyltransferase OS=Mus musculus OX=10090 GN=Trmo PE=2 SV=2)

HSP 1 Score: 141.0 bits (354), Expect = 2.8e-32
Identity = 70/150 (46.67%), Postives = 98/150 (65.33%), Query Frame = 0


Query: 92  PIGTIQSSFSTRNGTPRQSLVVPLARARLVFSSRI---PPASIEGLGEYSHCWVIYVFHL 151
           PIG ++S F  + GTPRQ  +   +RA L     I   P  S+ GL E+SH W+++VFH 
Sbjct: 32  PIGYLESCFPAKIGTPRQPSICSHSRACLKIRKNIFNNPEHSLMGLEEFSHVWILFVFHK 91

Query: 152 NTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGIFATRSPHRPCPIGITIAKVEAIQGHQIL 211
           N +L+          +KAKV+ PRL G K G+F+TRSPHRP  IG+T+AK+E ++G  + 
Sbjct: 92  NGHLN----------YKAKVQPPRLNGAKTGVFSTRSPHRPNAIGLTLAKLEKVEGGAVY 151

Query: 212 LSGADLVDGTPVLDVKPYLPYCDSIQDATV 239
           LSG D++DGTPVLD+KPY+   DS Q+ +V
Sbjct: 152 LSGVDMIDGTPVLDIKPYIADYDSPQNLSV 171

[Close]

BLAST of Cp4.1LG18g05910 vs. ExPASy Swiss-Prot
Match: Q4V7E0 (tRNA (adenine(37)-N6)-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trmo PE=2 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 4.0e-31
Identity = 68/147 (46.26%), Postives = 96/147 (65.31%), Query Frame = 0


Query: 92  PIGTIQSSFSTRNGTPRQSLVVPLARARLVFSSRI---PPASIEGLGEYSHCWVIYVFHL 151
           PIG ++S FS + GTPRQ  +   +RA L     I   P  S+ GL ++SH W+++VFH 
Sbjct: 32  PIGYLESCFSAKIGTPRQPSICSQSRACLKIRKSIFNNPEHSLMGLEQFSHVWILFVFHK 91

Query: 152 NTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGIFATRSPHRPCPIGITIAKVEAIQGHQIL 211
           N +L+          +KAKV+ PRL G K G+F+TRSPHRP  IG+T+AK+E ++G  + 
Sbjct: 92  NGHLN----------YKAKVQPPRLNGAKTGVFSTRSPHRPNAIGLTLAKLEKVEGGAVY 151

Query: 212 LSGADLVDGTPVLDVKPYLPYCDSIQD 236
           LSG D++ GTPVLD+KPY+   DS Q+
Sbjct: 152 LSGIDMIHGTPVLDIKPYIADYDSPQN 168

[Close]

BLAST of Cp4.1LG18g05910 vs. ExPASy Swiss-Prot
Match: Q9RPT0 (Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=rcsF PE=4 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 3.9e-26
Identity = 74/229 (32.31%), Postives = 120/229 (52.40%), Query Frame = 0


Query: 88  YPMAPIGTIQSSFSTRNGTPRQSLVVPLARARLVFSSRIPP----ASIEGLGEYSHCWVI 147
           + ++PIG I+S F  +   PRQ L+ P AR  L     +PP     ++EGL + SH W++
Sbjct: 3   HSVSPIGYIRSCFMEKFAIPRQPLLAPAARGTL---ELLPPFDQVEALEGLEQVSHVWLL 62

Query: 148 YVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEK-VGIFATRSPHRPCPIGITIAKVEAI 207
           ++FH             + K + KV+ PRL G + +G+FATR+ HRP  IG ++ ++E  
Sbjct: 63  FLFH----------QALEDKPRLKVRPPRLGGNRSLGVFATRATHRPNGIGQSVVRLEGF 122

Query: 208 QGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPKWLTGENVLSVASICFSEEFTPS 267
           +  ++ LSG DL+DGTPVLD+KPY+PY D++ DA         N ++ A         P 
Sbjct: 123 EAGRLWLSGIDLLDGTPVLDIKPYVPYADAVADA--------RNGIADAP-------PPG 182

Query: 268 LSECWPLIEKKSLYASADELK----NFVKQVLSWDIRSLSQRSQPHESY 308
           ++  W    ++  +     L+      ++Q L+ D R   Q+ +P   Y
Sbjct: 183 IAVEWSEQARRQAHEHGQRLRQPVAELIEQCLAQDPRPAYQKPEPGRRY 203

[Close]

BLAST of Cp4.1LG18g05910 vs. ExPASy Swiss-Prot
Match: O68638 (Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas aeruginosa OX=287 GN=rcsF PE=4 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 4.8e-24
Identity = 59/154 (38.31%), Postives = 93/154 (60.39%), Query Frame = 0


Query: 88  YPMAPIGTIQSSFSTRNGTPRQSLVVPLARARLVFSSRIPP----ASIEGLGEYSHCWVI 147
           + ++PIG ++S F  +   PRQ  + P A   L     +PP     ++EGL + SH W+I
Sbjct: 3   HSISPIGHVRSCFKEKFAIPRQPQLAPAATGVL---ELLPPFDTGDAVEGLEQVSHVWLI 62

Query: 148 YVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKG-EKVGIFATRSPHRPCPIGITIAKVEAI 207
           ++FH             + K + KV+ PRL G + +G+FATR+ HRP  +G ++ K+E +
Sbjct: 63  FLFH----------QALEDKPRLKVRPPRLGGNQSMGVFATRATHRPNGLGQSVVKLEKV 122

Query: 208 QGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDA 237
           +  ++ LSG DL+DGTPV+D+KPY+PY D + DA
Sbjct: 123 EPGRLWLSGIDLLDGTPVIDIKPYVPYADIVPDA 143

[Close]

BLAST of Cp4.1LG18g05910 vs. NCBI nr
Match: XP_023515484.1 (uncharacterized protein LOC111779629 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 724 bits (1870), Expect = 4.32e-263
Identity = 375/375 (100.00%), Postives = 375/375 (100.00%), Query Frame = 0


Query: 1   MAYAAVSRSSTAAMTVTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60
           MAYAAVSRSSTAAMTVTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE
Sbjct: 1   MAYAAVSRSSTAAMTVTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60

Query: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120
           RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120

Query: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180
           VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI
Sbjct: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180

Query: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK 240
           FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK
Sbjct: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK 240

Query: 241 WLTGENVLSVASICFSEEFTPSLSECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQR 300
           WLTGENVLSVASICFSEEFTPSLSECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQR
Sbjct: 241 WLTGENVLSVASICFSEEFTPSLSECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQR 300

Query: 301 SQPHESYLTTTRGHELSDASSDSDGHLDETLPSGTSQSSSKSVVYHLLVDGLNVSYTIGC 360
           SQPHESYLTTTRGHELSDASSDSDGHLDETLPSGTSQSSSKSVVYHLLVDGLNVSYTIGC
Sbjct: 301 SQPHESYLTTTRGHELSDASSDSDGHLDETLPSGTSQSSSKSVVYHLLVDGLNVSYTIGC 360

Query: 361 QGDVVVRKVELASPS 375
           QGDVVVRKVELASPS
Sbjct: 361 QGDVVVRKVELASPS 375

[Close]

BLAST of Cp4.1LG18g05910 vs. NCBI nr
Match: XP_022988427.1 (uncharacterized protein LOC111485678 [Cucurbita maxima])

HSP 1 Score: 716 bits (1849), Expect = 6.86e-260
Identity = 369/375 (98.40%), Postives = 373/375 (99.47%), Query Frame = 0


Query: 1   MAYAAVSRSSTAAMTVTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60
           MAYA VSRSSTAAM++TLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE
Sbjct: 1   MAYAVVSRSSTAAMSITLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60

Query: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120
           RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120

Query: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180
           VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI
Sbjct: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180

Query: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK 240
           FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK
Sbjct: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK 240

Query: 241 WLTGENVLSVASICFSEEFTPSLSECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQR 300
           WLTGENVLSVASICFSEEFTPSLSECWPLIEKKSLYASADELK+FVKQVLSWDIRSLSQR
Sbjct: 241 WLTGENVLSVASICFSEEFTPSLSECWPLIEKKSLYASADELKSFVKQVLSWDIRSLSQR 300

Query: 301 SQPHESYLTTTRGHELSDASSDSDGHLDETLPSGTSQSSSKSVVYHLLVDGLNVSYTIGC 360
           SQPHESYLTTTRGHELSD SSDSDGHLDETLPSGTSQSSSKSVVYHLLVDGLNVSYTIGC
Sbjct: 301 SQPHESYLTTTRGHELSDGSSDSDGHLDETLPSGTSQSSSKSVVYHLLVDGLNVSYTIGC 360

Query: 361 QGDVVVRKVELASPS 375
           QGDVVVRKVE+ASPS
Sbjct: 361 QGDVVVRKVEVASPS 375

[Close]

BLAST of Cp4.1LG18g05910 vs. NCBI nr
Match: XP_022965024.1 (uncharacterized protein LOC111464972 [Cucurbita moschata])

HSP 1 Score: 716 bits (1848), Expect = 9.75e-260
Identity = 370/375 (98.67%), Postives = 372/375 (99.20%), Query Frame = 0


Query: 1   MAYAAVSRSSTAAMTVTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60
           MAYAAVSRSSTAAM+VTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE
Sbjct: 1   MAYAAVSRSSTAAMSVTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60

Query: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120
           RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120

Query: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180
           VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI
Sbjct: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180

Query: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK 240
           FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK
Sbjct: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK 240

Query: 241 WLTGENVLSVASICFSEEFTPSLSECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQR 300
           W+TGENVLSVASICFSEEFTPSL ECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQR
Sbjct: 241 WITGENVLSVASICFSEEFTPSLLECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQR 300

Query: 301 SQPHESYLTTTRGHELSDASSDSDGHLDETLPSGTSQSSSKSVVYHLLVDGLNVSYTIGC 360
           SQPHESYLTTTRGHELSD SSDSDGHLDETLPSGTSQSSSK VVYHLLVDGLNVSYTIGC
Sbjct: 301 SQPHESYLTTTRGHELSDGSSDSDGHLDETLPSGTSQSSSKIVVYHLLVDGLNVSYTIGC 360

Query: 361 QGDVVVRKVELASPS 375
           QGDVVVRKVELASPS
Sbjct: 361 QGDVVVRKVELASPS 375

[Close]

BLAST of Cp4.1LG18g05910 vs. NCBI nr
Match: KAG7023410.1 (tRNA (adenine(37)-N6)-methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 716 bits (1847), Expect = 1.38e-259
Identity = 369/375 (98.40%), Postives = 372/375 (99.20%), Query Frame = 0


Query: 1   MAYAAVSRSSTAAMTVTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60
           MAYAAVSRSSTAAM+VTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE
Sbjct: 1   MAYAAVSRSSTAAMSVTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60

Query: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120
           RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120

Query: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180
           VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI
Sbjct: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180

Query: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK 240
           FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK
Sbjct: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK 240

Query: 241 WLTGENVLSVASICFSEEFTPSLSECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQR 300
           W+TGEN+LSVASICFSEEFTPSL ECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQR
Sbjct: 241 WITGENILSVASICFSEEFTPSLLECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQR 300

Query: 301 SQPHESYLTTTRGHELSDASSDSDGHLDETLPSGTSQSSSKSVVYHLLVDGLNVSYTIGC 360
           SQPHESYLTTTRGHELSD SSDSDGHLDETLPSGTSQSSSK VVYHLLVDGLNVSYTIGC
Sbjct: 301 SQPHESYLTTTRGHELSDGSSDSDGHLDETLPSGTSQSSSKIVVYHLLVDGLNVSYTIGC 360

Query: 361 QGDVVVRKVELASPS 375
           QGDVVVRKVELASPS
Sbjct: 361 QGDVVVRKVELASPS 375

[Close]

BLAST of Cp4.1LG18g05910 vs. NCBI nr
Match: XP_038879626.1 (tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 628 bits (1620), Expect = 6.42e-225
Identity = 315/375 (84.00%), Postives = 353/375 (94.13%), Query Frame = 0


Query: 1   MAYAAVSRSSTAAMTVTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60
           MAYAA SR S AA++VTLAF+SASA++SLY VTKKSRELG+KVRELEASLA+SLKKT AE
Sbjct: 1   MAYAAESRWSMAALSVTLAFISASAVISLYVVTKKSRELGTKVRELEASLASSLKKTAAE 60

Query: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120
           RQGRIRAQQALRKAV+EPKSDSVECT YPM PIGTI+SSFSTRNGTPRQSL+VPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARL 120

Query: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180
           VFS+RIPPASIEGLGEYSHCWVIYVFHLNT+LDKLWKDPSKSKFKAKV+VPRLKGE+VG+
Sbjct: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK 240
           FATRSPHRPCPIG+TIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDS+QDATVPK
Sbjct: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240

Query: 241 WLTGENVLSVASICFSEEFTPSLSECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQR 300
           WL G+N+L+VASICFSEEF P+LSECWPL+EKKSLYAS DE +NFVK+VLSWDIRS+SQR
Sbjct: 241 WLLGDNLLTVASICFSEEFIPTLSECWPLMEKKSLYASEDEFRNFVKEVLSWDIRSVSQR 300

Query: 301 SQPHESYLTTTRGHELSDASSDSDGHLDETLPSGTSQSSSKSVVYHLLVDGLNVSYTIGC 360
           +QPH+S++  T GHE  D SSDS+GHLD+ + SG SQSSS++++YHLLVDGL+VSYTIGC
Sbjct: 301 NQPHDSHVKPTTGHECFDGSSDSEGHLDDKVSSG-SQSSSENIIYHLLVDGLDVSYTIGC 360

Query: 361 QGDVVVRKVELASPS 375
            G+VVV KVELASPS
Sbjct: 361 HGNVVVGKVELASPS 374

[Close]

BLAST of Cp4.1LG18g05910 vs. ExPASy TrEMBL
Match: A0A6J1JCZ9 (uncharacterized protein LOC111485678 OS=Cucurbita maxima OX=3661 GN=LOC111485678 PE=4 SV=1)

HSP 1 Score: 716 bits (1849), Expect = 3.32e-260
Identity = 369/375 (98.40%), Postives = 373/375 (99.47%), Query Frame = 0


Query: 1   MAYAAVSRSSTAAMTVTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60
           MAYA VSRSSTAAM++TLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE
Sbjct: 1   MAYAVVSRSSTAAMSITLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60

Query: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120
           RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120

Query: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180
           VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI
Sbjct: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180

Query: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK 240
           FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK
Sbjct: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK 240

Query: 241 WLTGENVLSVASICFSEEFTPSLSECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQR 300
           WLTGENVLSVASICFSEEFTPSLSECWPLIEKKSLYASADELK+FVKQVLSWDIRSLSQR
Sbjct: 241 WLTGENVLSVASICFSEEFTPSLSECWPLIEKKSLYASADELKSFVKQVLSWDIRSLSQR 300

Query: 301 SQPHESYLTTTRGHELSDASSDSDGHLDETLPSGTSQSSSKSVVYHLLVDGLNVSYTIGC 360
           SQPHESYLTTTRGHELSD SSDSDGHLDETLPSGTSQSSSKSVVYHLLVDGLNVSYTIGC
Sbjct: 301 SQPHESYLTTTRGHELSDGSSDSDGHLDETLPSGTSQSSSKSVVYHLLVDGLNVSYTIGC 360

Query: 361 QGDVVVRKVELASPS 375
           QGDVVVRKVE+ASPS
Sbjct: 361 QGDVVVRKVEVASPS 375

[Close]

BLAST of Cp4.1LG18g05910 vs. ExPASy TrEMBL
Match: A0A6J1HPV8 (uncharacterized protein LOC111464972 OS=Cucurbita moschata OX=3662 GN=LOC111464972 PE=4 SV=1)

HSP 1 Score: 716 bits (1848), Expect = 4.72e-260
Identity = 370/375 (98.67%), Postives = 372/375 (99.20%), Query Frame = 0


Query: 1   MAYAAVSRSSTAAMTVTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60
           MAYAAVSRSSTAAM+VTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE
Sbjct: 1   MAYAAVSRSSTAAMSVTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60

Query: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120
           RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120

Query: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180
           VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI
Sbjct: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180

Query: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK 240
           FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK
Sbjct: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK 240

Query: 241 WLTGENVLSVASICFSEEFTPSLSECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQR 300
           W+TGENVLSVASICFSEEFTPSL ECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQR
Sbjct: 241 WITGENVLSVASICFSEEFTPSLLECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQR 300

Query: 301 SQPHESYLTTTRGHELSDASSDSDGHLDETLPSGTSQSSSKSVVYHLLVDGLNVSYTIGC 360
           SQPHESYLTTTRGHELSD SSDSDGHLDETLPSGTSQSSSK VVYHLLVDGLNVSYTIGC
Sbjct: 301 SQPHESYLTTTRGHELSDGSSDSDGHLDETLPSGTSQSSSKIVVYHLLVDGLNVSYTIGC 360

Query: 361 QGDVVVRKVELASPS 375
           QGDVVVRKVELASPS
Sbjct: 361 QGDVVVRKVELASPS 375

[Close]

BLAST of Cp4.1LG18g05910 vs. ExPASy TrEMBL
Match: A0A6J1BYL5 (uncharacterized protein LOC111006924 OS=Momordica charantia OX=3673 GN=LOC111006924 PE=4 SV=1)

HSP 1 Score: 619 bits (1597), Expect = 9.90e-222
Identity = 312/375 (83.20%), Postives = 347/375 (92.53%), Query Frame = 0


Query: 1   MAYAAVSRSSTAAMTVTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60
           MAY A SR STAAM++ LAF+SASAI+SLY VT+KSRELGSKVRELEASLAASLKKT AE
Sbjct: 1   MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAE 60

Query: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120
           RQGRIRAQQALRKA+++PKSD+VECT YPM PIG IQS+FSTRNGTPRQSLVVPLARARL
Sbjct: 61  RQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARL 120

Query: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180
           VFS+RIPPASIEGLGEYSHCW+IYVFHLNT+LDKLWK+PSKS+FKAKV+VPRLKG++VG+
Sbjct: 121 VFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGV 180

Query: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK 240
           FATRSPHRPCPIG+TIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDS+QDATVP+
Sbjct: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPE 240

Query: 241 WLTGENVLSVASICFSEEFTPSLSECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQR 300
           WL G+N+LSVASICFSEEFT +LS+CWPL+EKKS YASADE KNFVKQVLSWDIRSLSQR
Sbjct: 241 WLMGDNLLSVASICFSEEFTSTLSDCWPLMEKKSHYASADEFKNFVKQVLSWDIRSLSQR 300

Query: 301 SQPHESYLTTTRGHELSDASSDSDGHLDETLPSGTSQSSSKSVVYHLLVDGLNVSYTIGC 360
           ++PHES++ TT GHELSD SSD D HL +    GT Q SSK+++YHLLVDGLNVSYTIGC
Sbjct: 301 TRPHESHIETTTGHELSDGSSDLDTHLYDKASGGT-QISSKNIIYHLLVDGLNVSYTIGC 360

Query: 361 QGDVVVRKVELASPS 375
            GDVVV K ELASPS
Sbjct: 361 HGDVVVVKAELASPS 374

[Close]

BLAST of Cp4.1LG18g05910 vs. ExPASy TrEMBL
Match: A0A5D3CC84 (tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold499G00580 PE=4 SV=1)

HSP 1 Score: 615 bits (1587), Expect = 3.30e-220
Identity = 313/375 (83.47%), Postives = 348/375 (92.80%), Query Frame = 0


Query: 1   MAYAAVSRSSTAAMTVTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60
           MAYAA S  STAAM+V L F+SASAI+SLY VTKKSRELG+KV++LEASLAASLKKT AE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120
           RQGRIRAQQALRKAV+EPKSDSVECT YPM PIGTIQSSF+TRNGTPRQSL+VPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180
           VFS+RIPPASIEGL EYSHCWVIYVFHLNT+LDKLWKDPSKSKFKAKV+VPRLKGE+VG+
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK 240
           FATRSPHRPCPIG+T+AKVEA+QGH+ILLSGADLVDGTPVLDVKPYLPYCDS+QDATVPK
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQDATVPK 240

Query: 241 WLTGENVLSVASICFSEEFTPSLSECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQR 300
           WL G+NVL+VASICFSEEFT +LS+CWPL+EKKSLYAS +ELKNFVKQVLSWDIRS+SQR
Sbjct: 241 WLLGDNVLTVASICFSEEFTSALSKCWPLVEKKSLYASEEELKNFVKQVLSWDIRSVSQR 300

Query: 301 SQPHESYLTTTRGHELSDASSDSDGHLDETLPSGTSQSSSKSVVYHLLVDGLNVSYTIGC 360
           S+PH S++ T  G EL D SSDSDGH+D+ + S +SQSSSKS +YHLLVDGL+VSYTIG 
Sbjct: 301 SRPHCSHIKTVTGDELLDESSDSDGHVDDKV-SSSSQSSSKSTIYHLLVDGLDVSYTIGY 360

Query: 361 QGDVVVRKVELASPS 375
            GDVVV KVELASPS
Sbjct: 361 HGDVVVDKVELASPS 374

[Close]

BLAST of Cp4.1LG18g05910 vs. ExPASy TrEMBL
Match: A0A0A0LTM6 (TsaA-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G295140 PE=4 SV=1)

HSP 1 Score: 612 bits (1578), Expect = 6.44e-219
Identity = 314/375 (83.73%), Postives = 348/375 (92.80%), Query Frame = 0


Query: 1   MAYAAVSRSSTAAMTVTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60
           M YAA SR STAAM+VTLAF+SASAI+SLY VTKKSRELG+KVR+LEASLAASLKKT AE
Sbjct: 1   MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 60

Query: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120
           RQGRIRAQQALRKAV+EPKSDSV+CT YPM PIGTIQSSF+TRNGTPRQSL+VPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180
           VFS+RIPPASIEGLGEYSHCWVIYVFHLNT+LDKLWKDPSKSKFKAKV+VPRLKGE+VG+
Sbjct: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPK 240
           FATRSPHRPCPIG+T+AKVEA+QGH+ILLSGADLVDGTPVLDVKPYLPYCDS+Q+ATVPK
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQNATVPK 240

Query: 241 WLTGENVLSVASICFSEEFTPSLSECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQR 300
           WL G+NVL+VASICFSEEFT +LS+CWPL EKKSLYAS DELKNFVKQVLSWDIRS+SQR
Sbjct: 241 WLLGDNVLTVASICFSEEFTSTLSKCWPLTEKKSLYASEDELKNFVKQVLSWDIRSVSQR 300

Query: 301 SQPHESYLTTTRGHELSDASSDSDGHLDETLPSGTSQSSSKSVVYHLLVDGLNVSYTIGC 360
           S+PH S++ T      SD SSDSDGHL++ + S +SQSSSKS +YHLLVDGL+VSYTIGC
Sbjct: 301 SRPHCSHIKTD-----SDDSSDSDGHLNDKV-SSSSQSSSKSTIYHLLVDGLDVSYTIGC 360

Query: 361 QGDVVVRKVELASPS 375
            GDVVV KVELAS S
Sbjct: 361 HGDVVVDKVELASSS 369

[Close]

BLAST of Cp4.1LG18g05910 vs. TAIR 10
Match: AT4G28020.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Uncharacterised protein family UPF0066 (InterPro:IPR001378); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 397.9 bits (1021), Expect = 9.0e-111
Identity = 199/364 (54.67%), Postives = 271/364 (74.45%), Query Frame = 0


Query: 9   SSTAAMTVTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAERQGRIRAQ 68
           S+TAA+  TLA VS SA  SLY   ++  +L ++++E E SL ++L+K+ +ER+GR++AQ
Sbjct: 10  STTAALAFTLAVVSVSAAFSLYRWRRREEDLENRIKEFEKSLGSTLEKSASERKGRVKAQ 69

Query: 69  QALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARLVFS-SRIP 128
           QALR+A+++ +S  +  T+YPM PIGTIQS FSTRNGTPRQ L+V LARA L+F  + +P
Sbjct: 70  QALREALAQTESHDLHSTIYPMRPIGTIQSCFSTRNGTPRQPLLVSLARACLIFDPALVP 129

Query: 129 PASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGIFATRSPH 188
           PAS+EGL EYSHCW++YVFHLNT+++KLW+ PS+SK KAKV+VPRL GE+ G+FATRSPH
Sbjct: 130 PASLEGLEEYSHCWILYVFHLNTDIEKLWRKPSQSKLKAKVRVPRLNGERKGVFATRSPH 189

Query: 189 RPCPIGITIAKVEAIQGHQILLSGADLVDGTPVLDVKPYLPYCDSIQDATVPKWLTGENV 248
           RPCPIG+T+AKVE IQ  ++LLSG DLVDGTPVLD+KPYLPY DSIQ A+VP W+  +  
Sbjct: 190 RPCPIGLTVAKVEEIQKDKVLLSGVDLVDGTPVLDIKPYLPYSDSIQGASVPNWVKEDCS 249

Query: 249 LSVASICFSEEFTPSLSECWPLIEKKSLYASADELKNFVKQVLSWDIRSLSQRSQPHESY 308
           L+VAS+ FS+ F+ S++ CW LIEKKSLY+SADE ++ + QVLSWDIRS+SQR++P ++ 
Sbjct: 250 LAVASVTFSDTFSSSITSCWKLIEKKSLYSSADEFRSLITQVLSWDIRSMSQRNKPQDT- 309

Query: 309 LTTTRGHELSDASSDSDGHLDETLPSGTSQSSSKSVVYHLLVDGLNVSYTIGCQGDVVVR 368
                              LDE             +VYHL+++GL+VSY I  + +++V+
Sbjct: 310 -------------------LDE------------EIVYHLVLEGLDVSYMIDNESNILVQ 341

Query: 369 KVEL 372
            V L
Sbjct: 370 DVSL 341

The following BLAST results are available for this feature:

BLAST of Cp4.1LG18g05910 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9BU70	1.3e-32	47.02	tRNA (adenine(37)-N6)-methyltransferase OS=Homo sapiens OX=9606 GN=TRMO PE=1 SV=...	[more]
Q562D6	2.8e-32	46.67	tRNA (adenine(37)-N6)-methyltransferase OS=Mus musculus OX=10090 GN=Trmo PE=2 SV...	[more]
Q4V7E0	4.0e-31	46.26	tRNA (adenine(37)-N6)-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trmo PE...	[more]
Q9RPT0	3.9e-26	32.31	Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas ae...	[more]
O68638	4.8e-24	38.31	Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas ae...	[more]

BLAST of Cp4.1LG18g05910 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023515484.1	4.32e-263	100.00	uncharacterized protein LOC111779629 [Cucurbita pepo subsp. pepo]	[more]
XP_022988427.1	6.86e-260	98.40	uncharacterized protein LOC111485678 [Cucurbita maxima]	[more]
XP_022965024.1	9.75e-260	98.67	uncharacterized protein LOC111464972 [Cucurbita moschata]	[more]
KAG7023410.1	1.38e-259	98.40	tRNA (adenine(37)-N6)-methyltransferase [Cucurbita argyrosperma subsp. argyrospe...	[more]
XP_038879626.1	6.42e-225	84.00	tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Benincasa hispida]	[more]

BLAST of Cp4.1LG18g05910 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JCZ9	3.32e-260	98.40	uncharacterized protein LOC111485678 OS=Cucurbita maxima OX=3661 GN=LOC111485678...	[more]
A0A6J1HPV8	4.72e-260	98.67	uncharacterized protein LOC111464972 OS=Cucurbita moschata OX=3662 GN=LOC1114649...	[more]
A0A6J1BYL5	9.90e-222	83.20	uncharacterized protein LOC111006924 OS=Momordica charantia OX=3673 GN=LOC111006...	[more]
A0A5D3CC84	3.30e-220	83.47	tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa O...	[more]
A0A0A0LTM6	6.44e-219	83.73	TsaA-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G295140 P...	[more]

BLAST of Cp4.1LG18g05910 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G28020.1	9.0e-111	54.67	unknown protein; CONTAINS InterPro DOMAIN/s: Uncharacterised protein family UPF0...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036414	YaeB, N-terminal domain superfamily	GENE3D	2.40.30.70		coord: 89..244 e-value: 2.5E-52 score: 178.5
IPR023370	TrmO-like, N-terminal domain	PFAM	PF01980	TrmO	coord: 105..231 e-value: 8.7E-42 score: 142.1
IPR023370	TrmO-like, N-terminal domain	PROSITE	PS51668	TSAA_2	coord: 90..235 score: 65.84938
IPR023370	TrmO-like, N-terminal domain	CDD	cd09281	UPF0066	coord: 92..226 e-value: 7.47273E-49 score: 159.194
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 303..338
None	No IPR available	PANTHER	PTHR12818:SF0	TRNA (ADENINE(37)-N6)-METHYLTRANSFERASE	coord: 8..371
IPR040372	YaeB-like	PANTHER	PTHR12818	UNCHARACTERIZED	coord: 8..371
IPR036413	YaeB-like superfamily	SUPERFAMILY	118196	YaeB-like	coord: 87..307

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG18g05910.1	Cp4.1LG18g05910.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030488	tRNA methylation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016430	tRNA (adenine-N6-)-methyltransferase activity