Cp4.1LG18g03840 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG18g03840
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionInositol oxygenase
LocationCp4.1LG18: 5130161 .. 5132786 (+)
RNA-Seq ExpressionCp4.1LG18g03840
SyntenyCp4.1LG18g03840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATGTGGTCGAGAATCAACCACGATCTTGGAGTGAAGATTGTTTCGAGACGGTTTTTAGGATGTACTCATTATCTTTGTGCTGCCGGATCGATCATCTAACTATATTTTGACACCAGATAAATTAAAAAAATAATAATATCTTGAAAATAAAGACAAAAGAAAATAGAAAGAAAAAAAATTGTTTACATTTAAGGACTCCAAAAGTTTCAAATTGTCATGGAAAGGACAGCTTGGTGAGTATAAAGAAACCCCCTCCACCAATTCTCCTCACACCGAATCTTATTCATATTCCGTTCTTTTGGATTCTTTGATTCTTGTGAAGAACATGACGATCCTCATCGAGCAGCCTCGCTTTGGTAACATCTTCTTGTTCTTCACCATTTACTTTTCATAAACATTTGAATTTTCAGCGAATTCTTGAGGGTTCTTAATGTTCTTGTATTTGCAGAAGTGGAAGAGAAGAAGGTTGGCATTACAGAGCTGAATGAATTGGTGTTGGACGGTGGATTTGTTGTACCAAATGAAACGGCAGCGGCGGCGGCGGAGGAGGGATTTGTAGCACTGGAAATGAATGCGTTCGGGCATTCTTTCAGAGATTACGAAGCGGAAAGTGAAAGGCAAAAGGGAGTGGAGGAATTTTATAAACAGAATCATATTCATCAAACGTACGATTACGTGAAGAAGATGAGAGAAACGTACAAGAAAATGGATCGAGTGGAAATGAGTATCTGGGAATGCTGTGAGCTTCTGAACGATGTGGTTGATGATAGCGATCCTGATTTGGATGAACCCCAAATTCAGCATTTGTTGCAGACTGCTGAAGCCATTCGAAAGGATTATCCTGATGAAGATTGGCTGCATTTGACTGCGCTCATCCATGGTATTATATCATCAAATTCCCTTCGACTCTGTTTTCTCTCTGTTTCTCTCTGTTTCACTCTGTAACGAAATGCTCTGTTTTTAGATCTTGGAAAAGTGCTTCTTCTTCCCAGCTTTGGTGGGCTTCCTCAATGGGCTGTTGTGGGTAAGAACAAACAACAAAACCCCATAATTTTTCTATCATTTTCTTCCCAAATAATCAAACACAACACTCAAATCCATCTTCCCCTGTTTTGTTCTTGCAGGTGATACACACCCTCTAGGCTGCGCTTTTGACAGCTCCATTGTTCATCACAAGGTAGTTCATAATTCTGTAAATTCGAGATTTCTTTCCATTAAATTACGAACAATAAAACTAATTTATTTGGGTTTTGATTTCAGTATTTCAAGGAGAATCCTGATTCAGAGAATCCAGCTTATAACACCAAAAATGGAATTTACACCGAAGGCTGCGGACTTGACAATGTTATGATATCATGGGGCCATGATGATTATATGTATTTGGTACCAATTCCATTCCATTTGCTCCTTTTCTGGTAAAGCTTTGATTTTCGAAGCTCTGTTTGTTTAACAATGGTGTCTGATGTAAATTTTGCAGGTGGCTAAAGAAAATGGCTCCACTTTACCTTCGGCTGGGTTGTTCATAATCAGATACCATTCTTTTTATCGTAAGCAAATAAGATTCAAACTTAAAATTTGAATTTTGGGTGATGAATTGAAACTAAATGAATTCATTTTCCTGCAGCCTTACATAAAGCAGGAGCTTACAAACACTTGATGAATGAGGAGGATGCCCAGAATTTGAAGTGGCTCCATATATTCAAGTAATTATTTAAAAAAATTACTGTTTTAATCCAAGATAATTGGAGTGATTATGAACTAATTATATATTTATGGAATTTCAGCAAGTATGATCTCTACAGTAAGAGCAAAGAACTTATTGACGTGGAAAAAGTTAAACCATATTATGTTTCCCTAATTAACAAGGTAAATCCTGTGACTTTTTTTATTTTTTTTCCAAATATTTTGGGGATTAAATGGTGTAAATTATTGGGATTTGCAGTACTTCCCGGAAAAGCTCCGATGGTGACCAGATATAATTAACGATGACTATCGCCGGCCGGCGACGGATGACACATTTGAATGAATAAGCTTCATTTTTCTTGTATTATTATCTCATTTGTCCACATTAAATGAGTTTCTTTTTTTTTTCTCTTTCTTTTTTTCCTTAAATGTAATGGAAAAAAATTGCGTCTTGCAAAAGCAAATTAAAAATTTAATTGTTTTTGTTCTCCCATGTGTTTGACCGTAACAGAGTTTGTAAGTTATAATTAATTATTATCTAAAAAAATTAAGAATATTATTGTTATAATGTACTAATTTCATTCAATATTTGACACACTTAATTTATTAAATGATGTATTATATTTTAATGTGGATTAGTTTATAATGTCAACTTCATTAAAAAATTATTTATAAGATTTTATGAAGAATAAAATTTTAATTTTTTAAATGATACAGACTAAATTATAAGTTTCTTAAATTAGGAATAAAATTACAATTTGAGTAGAAATACGATTAAAGATGAAAATGAAAGCTTGTTTAATGTTATAAAATATATAAATGATGTCATTTAATTAATCAACTTTCTAAATATCTAAGTTATAATATTAGAAAGAGCAATGGTGAAATTCAAAAAATTATAGAATTATTATTATTTTTATTCATATGAGTGATTATTCTAA

mRNA sequence

AAATGTGGTCGAGAATCAACCACGATCTTGGAGTGAAGATTGTTTCGAGACGGTTTTTAGGATGTACTCATTATCTTTGTGCTGCCGGATCGATCATCTAACTATATTTTGACACCAGATAAATTAAAAAAATAATAATATCTTGAAAATAAAGACAAAAGAAAATAGAAAGAAAAAAAATTGTTTACATTTAAGGACTCCAAAAGTTTCAAATTGTCATGGAAAGGACAGCTTGGTGAGTATAAAGAAACCCCCTCCACCAATTCTCCTCACACCGAATCTTATTCATATTCCGTTCTTTTGGATTCTTTGATTCTTGTGAAGAACATGACGATCCTCATCGAGCAGCCTCGCTTTGAAGTGGAAGAGAAGAAGGTTGGCATTACAGAGCTGAATGAATTGGTGTTGGACGGTGGATTTGTTGTACCAAATGAAACGGCAGCGGCGGCGGCGGAGGAGGGATTTGTAGCACTGGAAATGAATGCGTTCGGGCATTCTTTCAGAGATTACGAAGCGGAAAGTGAAAGGCAAAAGGGAGTGGAGGAATTTTATAAACAGAATCATATTCATCAAACGTACGATTACGTGAAGAAGATGAGAGAAACGTACAAGAAAATGGATCGAGTGGAAATGAGTATCTGGGAATGCTGTGAGCTTCTGAACGATGTGGTTGATGATAGCGATCCTGATTTGGATGAACCCCAAATTCAGCATTTGTTGCAGACTGCTGAAGCCATTCGAAAGGATTATCCTGATGAAGATTGGCTGCATTTGACTGCGCTCATCCATGATCTTGGAAAAGTGCTTCTTCTTCCCAGCTTTGGTGGGCTTCCTCAATGGGCTGTTGTGGGTGATACACACCCTCTAGGCTGCGCTTTTGACAGCTCCATTGTTCATCACAAGTATTTCAAGGAGAATCCTGATTCAGAGAATCCAGCTTATAACACCAAAAATGGAATTTACACCGAAGGCTGCGGACTTGACAATGTTATGATATCATGGGGCCATGATGATTATATGTATTTGGTGGCTAAAGAAAATGGCTCCACTTTACCTTCGGCTGGGTTGTTCATAATCAGATACCATTCTTTTTATCCCTTACATAAAGCAGGAGCTTACAAACACTTGATGAATGAGGAGGATGCCCAGAATTTGAAGTGGCTCCATATATTCAACAAGTATGATCTCTACAGTAAGAGCAAAGAACTTATTGACGTGGAAAAAGTTAAACCATATTATGTTTCCCTAATTAACAAGTACTTCCCGGAAAAGCTCCGATGGTGACCAGATATAATTAACGATGACTATCGCCGGCCGGCGACGGATGACACATTTGAATGAATAAGCTTCATTTTTCTTGTATTATTATCTCATTTGTCCACATTAAATGAGTTTCTTTTTTTTTTCTCTTTCTTTTTTTCCTTAAATGTAATGGAAAAAAATTGCGTCTTGCAAAAGCAAATTAAAAATTTAATTGTTTTTGTTCTCCCATGTGTTTGACCGTAACAGAGTTTGTAAGTTATAATTAATTATTATCTAAAAAAATTAAGAATATTATTGTTATAATGTACTAATTTCATTCAATATTTGACACACTTAATTTATTAAATGATGTATTATATTTTAATGTGGATTAGTTTATAATGTCAACTTCATTAAAAAATTATTTATAAGATTTTATGAAGAATAAAATTTTAATTTTTTAAATGATACAGACTAAATTATAAGTTTCTTAAATTAGGAATAAAATTACAATTTGAGTAGAAATACGATTAAAGATGAAAATGAAAGCTTGTTTAATGTTATAAAATATATAAATGATGTCATTTAATTAATCAACTTTCTAAATATCTAAGTTATAATATTAGAAAGAGCAATGGTGAAATTCAAAAAATTATAGAATTATTATTATTTTTATTCATATGAGTGATTATTCTAA

Coding sequence (CDS)

ATGACGATCCTCATCGAGCAGCCTCGCTTTGAAGTGGAAGAGAAGAAGGTTGGCATTACAGAGCTGAATGAATTGGTGTTGGACGGTGGATTTGTTGTACCAAATGAAACGGCAGCGGCGGCGGCGGAGGAGGGATTTGTAGCACTGGAAATGAATGCGTTCGGGCATTCTTTCAGAGATTACGAAGCGGAAAGTGAAAGGCAAAAGGGAGTGGAGGAATTTTATAAACAGAATCATATTCATCAAACGTACGATTACGTGAAGAAGATGAGAGAAACGTACAAGAAAATGGATCGAGTGGAAATGAGTATCTGGGAATGCTGTGAGCTTCTGAACGATGTGGTTGATGATAGCGATCCTGATTTGGATGAACCCCAAATTCAGCATTTGTTGCAGACTGCTGAAGCCATTCGAAAGGATTATCCTGATGAAGATTGGCTGCATTTGACTGCGCTCATCCATGATCTTGGAAAAGTGCTTCTTCTTCCCAGCTTTGGTGGGCTTCCTCAATGGGCTGTTGTGGGTGATACACACCCTCTAGGCTGCGCTTTTGACAGCTCCATTGTTCATCACAAGTATTTCAAGGAGAATCCTGATTCAGAGAATCCAGCTTATAACACCAAAAATGGAATTTACACCGAAGGCTGCGGACTTGACAATGTTATGATATCATGGGGCCATGATGATTATATGTATTTGGTGGCTAAAGAAAATGGCTCCACTTTACCTTCGGCTGGGTTGTTCATAATCAGATACCATTCTTTTTATCCCTTACATAAAGCAGGAGCTTACAAACACTTGATGAATGAGGAGGATGCCCAGAATTTGAAGTGGCTCCATATATTCAACAAGTATGATCTCTACAGTAAGAGCAAAGAACTTATTGACGTGGAAAAAGTTAAACCATATTATGTTTCCCTAATTAACAAGTACTTCCCGGAAAAGCTCCGATGGTGA

Protein sequence

MTILIEQPRFEVEEKKVGITELNELVLDGGFVVPNETAAAAAEEGFVALEMNAFGHSFRDYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEKVKPYYVSLINKYFPEKLRW
Homology
BLAST of Cp4.1LG18g03840 vs. ExPASy Swiss-Prot
Match: O82200 (Inositol oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=MIOX2 PE=2 SV=2)

HSP 1 Score: 515.8 bits (1327), Expect = 3.5e-145
Identity = 245/321 (76.32%), Postives = 276/321 (85.98%), Query Frame = 0

Query: 1   MTILIEQ--PRFEVEEKKVGITELNELVLDGGFVVPNETAAAAAEEGFVALEMNAFGHSF 60
           MTIL+E   P   V+EKKV     NELVLDGGFVVP     +   + F A +MN  GHSF
Sbjct: 1   MTILVEHFVPDSRVDEKKVIEERDNELVLDGGFVVPK----SKETDAFDAPDMNFLGHSF 60

Query: 61  RDYE-AESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDD 120
           RDYE  ESERQ+GVEEFY+  HIHQTYD+VKKMR+ Y K++++EMSIWECCELLN+VVD+
Sbjct: 61  RDYENGESERQQGVEEFYRMQHIHQTYDFVKKMRKEYGKLNKMEMSIWECCELLNNVVDE 120

Query: 121 SDPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDT 180
           SDPDLDEPQIQHLLQTAEAIR+DYPDEDWLHLTALIHDLGKVLLLP FGGLPQWAVVGDT
Sbjct: 121 SDPDLDEPQIQHLLQTAEAIRRDYPDEDWLHLTALIHDLGKVLLLPEFGGLPQWAVVGDT 180

Query: 181 HPLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKE 240
            P+GC FDS+ +HHKYFK N D  NP YNTKNG+YTEGCGLDNV++SWGHDDYMYLVAK+
Sbjct: 181 FPVGCTFDSANIHHKYFKGNHDINNPKYNTKNGVYTEGCGLDNVLMSWGHDDYMYLVAKK 240

Query: 241 NGSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDV 300
           NG+TLP AGLFIIRYHSFYPLHKAGAY HLMN+ED  +LKWLH+FNKYDLYSKSK L+DV
Sbjct: 241 NGTTLPHAGLFIIRYHSFYPLHKAGAYTHLMNDEDRDDLKWLHVFNKYDLYSKSKVLVDV 300

Query: 301 EKVKPYYVSLINKYFPEKLRW 319
           E+VKPYY+SLINKYFP KL+W
Sbjct: 301 EQVKPYYISLINKYFPAKLKW 317

BLAST of Cp4.1LG18g03840 vs. ExPASy Swiss-Prot
Match: Q8H1S0 (Inositol oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=MIOX4 PE=2 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 3.4e-140
Identity = 239/319 (74.92%), Postives = 266/319 (83.39%), Query Frame = 0

Query: 1   MTILIEQPRF-EVEEKKVGITELNELVLDGGFVVPNETAAAAAEEGFVALEMNAFGHSFR 60
           MTI +E+P F EV   +     + EL LDGGF +P        +E F+A EMNAFG  FR
Sbjct: 1   MTISVEKPIFEEVSAFEKSGDNIGELKLDGGFSMPK--MDTNDDEAFLAPEMNAFGRQFR 60

Query: 61  DYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSD 120
           DY+ ESERQKGVEEFY+  HI+QT D+VKKMR  Y K+D++ MSIWECCELLN+VVD+SD
Sbjct: 61  DYDVESERQKGVEEFYRLQHINQTVDFVKKMRAEYGKLDKMVMSIWECCELLNEVVDESD 120

Query: 121 PDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHP 180
           PDLDEPQIQHLLQ+AEAIRKDYP+EDWLHLTALIHDLGKV+ LP FGGLPQWAVVGDT P
Sbjct: 121 PDLDEPQIQHLLQSAEAIRKDYPNEDWLHLTALIHDLGKVITLPQFGGLPQWAVVGDTFP 180

Query: 181 LGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENG 240
           +GCAFD S VHHKYF ENPD  N  YNTKNGIY+EGCGL+NVM+SWGHDDYMYLVAKENG
Sbjct: 181 VGCAFDESNVHHKYFVENPDFHNETYNTKNGIYSEGCGLNNVMMSWGHDDYMYLVAKENG 240

Query: 241 STLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEK 300
           STLPSAG FIIRYHSFYPLH AG Y HLMNEED +NLKWLH+FNKYDLYSKSK  +DVEK
Sbjct: 241 STLPSAGQFIIRYHSFYPLHTAGEYTHLMNEEDKENLKWLHVFNKYDLYSKSKVHVDVEK 300

Query: 301 VKPYYVSLINKYFPEKLRW 319
           VKPYY+SLI KYFPE LRW
Sbjct: 301 VKPYYMSLIKKYFPENLRW 317

BLAST of Cp4.1LG18g03840 vs. ExPASy Swiss-Prot
Match: Q8L799 (Inositol oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=MIOX1 PE=2 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 1.2e-132
Identity = 229/326 (70.25%), Postives = 264/326 (80.98%), Query Frame = 0

Query: 1   MTILIEQ------PRFEVEEKKV--GITELNELVLDGGFVVPNETAAAAAEEGFVALEMN 60
           MTILI++         E+ EK    G  E  ELVLD GF  P+                N
Sbjct: 1   MTILIDRHSDQNDAGDEIVEKNQGNGKEEETELVLDAGFEAPH---------------TN 60

Query: 61  AFGHSFRDYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLN 120
           +FG +FRDY+AESER++GVEEFY+ NHI QT D+V+KMRE Y+K++R EMSIWECCELLN
Sbjct: 61  SFGRTFRDYDAESERRRGVEEFYRVNHIGQTVDFVRKMREEYEKLNRTEMSIWECCELLN 120

Query: 121 DVVDDSDPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWA 180
           + +D+SDPDLDEPQI+HLLQTAEAIRKDYPDEDWLHLT LIHDLGKVLL  SFG LPQWA
Sbjct: 121 EFIDESDPDLDEPQIEHLLQTAEAIRKDYPDEDWLHLTGLIHDLGKVLLHSSFGELPQWA 180

Query: 181 VVGDTHPLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMY 240
           VVGDT P+GCAFD SIVHHKYFKENPD +NP+YN+K GIYTEGCGLDNV++SWGHDDYMY
Sbjct: 181 VVGDTFPVGCAFDESIVHHKYFKENPDYDNPSYNSKYGIYTEGCGLDNVLMSWGHDDYMY 240

Query: 241 LVAKENGSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSK 300
           LVAKEN +TLPSAGLFIIRYHSFY LHK+ AYKHLMN ED +N+KWL +FNKYDLYSKSK
Sbjct: 241 LVAKENQTTLPSAGLFIIRYHSFYALHKSEAYKHLMNNEDRENMKWLKVFNKYDLYSKSK 300

Query: 301 ELIDVEKVKPYYVSLINKYFPEKLRW 319
             ++VE+VKPYY+SL NKYFP KL+W
Sbjct: 301 VRVNVEEVKPYYLSLTNKYFPSKLKW 311

BLAST of Cp4.1LG18g03840 vs. ExPASy Swiss-Prot
Match: Q5Z8T3 (Probable inositol oxygenase OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0561000 PE=2 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 5.9e-132
Identity = 229/323 (70.90%), Postives = 261/323 (80.80%), Query Frame = 0

Query: 1   MTILIEQPRFE-VEEKKV----GITELNELVLDGGFVVPNETAAAAAEEGFVALEMNAFG 60
           MTI IEQP  + + ++KV    G     ELVLDGGFVVP               + NAFG
Sbjct: 1   MTITIEQPHLDAIADRKVAGGGGGDNAAELVLDGGFVVP---------------DSNAFG 60

Query: 61  HSFRDYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVV 120
           ++FR+YEAESER++ VEEFY+ NHI+QTYD+V++MRE Y ++D+ EM IWEC ELLN+ +
Sbjct: 61  NAFRNYEAESERKETVEEFYRVNHINQTYDFVRRMREEYGRVDKTEMGIWECIELLNEFI 120

Query: 121 DDSDPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVG 180
           DDSDPDLD PQI+HLLQTAEAIRKD+PDEDWLHLT LIHDLGKVLL PSFG LPQW+VVG
Sbjct: 121 DDSDPDLDMPQIEHLLQTAEAIRKDFPDEDWLHLTGLIHDLGKVLLHPSFGELPQWSVVG 180

Query: 181 DTHPLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVA 240
           DT P+GCAFD   VH KYFKENPD  NP  NTK G Y+EGCGLDNV++SWGHDDYMYLVA
Sbjct: 181 DTFPVGCAFDECNVHFKYFKENPDYLNPKLNTKFGAYSEGCGLDNVLMSWGHDDYMYLVA 240

Query: 241 KENGSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELI 300
           KEN +TLPSAGLFIIRYHSFYPLHK GAY HLMN+ED +NLKWL +FNKYDLYSKS E I
Sbjct: 241 KENKTTLPSAGLFIIRYHSFYPLHKHGAYMHLMNDEDKENLKWLRVFNKYDLYSKSNERI 300

Query: 301 DVEKVKPYYVSLINKYFPEKLRW 319
           DVEKVKPYY+SLI KYFP KLRW
Sbjct: 301 DVEKVKPYYMSLIEKYFPAKLRW 308

BLAST of Cp4.1LG18g03840 vs. ExPASy Swiss-Prot
Match: Q9FJU4 (Inositol oxygenase 5 OS=Arabidopsis thaliana OX=3702 GN=MIOX5 PE=2 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 3.8e-131
Identity = 227/319 (71.16%), Postives = 257/319 (80.56%), Query Frame = 0

Query: 1   MTILIEQPRFEVEEKKVGITELNELVLDGGFVVPNETAAAAAEEGFVALEMNAFGHSFRD 60
           M I +E P F  E+     T   EL LD    +   +  ++ +E F+A EMNAFG  FRD
Sbjct: 1   MNISVENPVFVHEDSTTQKT--GELRLDSDIPM---SKISSDDEVFLAPEMNAFGRQFRD 60

Query: 61  Y-EAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSD 120
           Y +  SERQK VE FY   H +QT D+V+KMR  Y K+D++ M+IWECCEL  +VVD+SD
Sbjct: 61  YTDTNSERQKSVEHFYATQHTNQTLDFVQKMRSEYGKLDKMVMNIWECCELSKEVVDESD 120

Query: 121 PDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHP 180
           PDLDEPQIQHLLQ+AEAIRKDYP+EDWLHLTALIHDLGKVL LP FGGLPQWAVVGDT P
Sbjct: 121 PDLDEPQIQHLLQSAEAIRKDYPNEDWLHLTALIHDLGKVLTLPQFGGLPQWAVVGDTFP 180

Query: 181 LGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENG 240
           +GCAFD S VHHKYF ENPD  NP YNTK GIY+EGCGL+NV++SWGHDDYMYLVAKENG
Sbjct: 181 VGCAFDESNVHHKYFMENPDFNNPKYNTKAGIYSEGCGLENVLMSWGHDDYMYLVAKENG 240

Query: 241 STLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEK 300
           STLPS GLFIIRYHSFYPLHKAGAY HLMNEED +NLKWLH+FNKYDLYSKSK  ++VEK
Sbjct: 241 STLPSPGLFIIRYHSFYPLHKAGAYTHLMNEEDKENLKWLHVFNKYDLYSKSKVHVNVEK 300

Query: 301 VKPYYVSLINKYFPEKLRW 319
           VKPYY+SLI KYFPE LRW
Sbjct: 301 VKPYYMSLIKKYFPENLRW 314

BLAST of Cp4.1LG18g03840 vs. NCBI nr
Match: XP_023515618.1 (inositol oxygenase 2-like [Cucurbita pepo subsp. pepo] >KAG7023630.1 Inositol oxygenase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 658 bits (1697), Expect = 1.19e-238
Identity = 318/318 (100.00%), Postives = 318/318 (100.00%), Query Frame = 0

Query: 1   MTILIEQPRFEVEEKKVGITELNELVLDGGFVVPNETAAAAAEEGFVALEMNAFGHSFRD 60
           MTILIEQPRFEVEEKKVGITELNELVLDGGFVVPNETAAAAAEEGFVALEMNAFGHSFRD
Sbjct: 1   MTILIEQPRFEVEEKKVGITELNELVLDGGFVVPNETAAAAAEEGFVALEMNAFGHSFRD 60

Query: 61  YEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDP 120
           YEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDP
Sbjct: 61  YEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDP 120

Query: 121 DLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPL 180
           DLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPL
Sbjct: 121 DLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPL 180

Query: 181 GCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGS 240
           GCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGS
Sbjct: 181 GCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGS 240

Query: 241 TLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEKV 300
           TLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEKV
Sbjct: 241 TLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEKV 300

Query: 301 KPYYVSLINKYFPEKLRW 318
           KPYYVSLINKYFPEKLRW
Sbjct: 301 KPYYVSLINKYFPEKLRW 318

BLAST of Cp4.1LG18g03840 vs. NCBI nr
Match: XP_022960634.1 (inositol oxygenase 2-like [Cucurbita moschata] >KAG6589967.1 Inositol oxygenase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 650 bits (1676), Expect = 1.76e-235
Identity = 316/318 (99.37%), Postives = 316/318 (99.37%), Query Frame = 0

Query: 1   MTILIEQPRFEVEEKKVGITELNELVLDGGFVVPNETAAAAAEEGFVALEMNAFGHSFRD 60
           MTILIEQPRFEVEEKKVGITELNELVLDGGFVVPNETAAA  EEGFVALEMNAFGHSFRD
Sbjct: 1   MTILIEQPRFEVEEKKVGITELNELVLDGGFVVPNETAAA--EEGFVALEMNAFGHSFRD 60

Query: 61  YEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDP 120
           YEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDP
Sbjct: 61  YEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDP 120

Query: 121 DLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPL 180
           DLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPL
Sbjct: 121 DLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPL 180

Query: 181 GCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGS 240
           GCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGS
Sbjct: 181 GCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGS 240

Query: 241 TLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEKV 300
           TLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEKV
Sbjct: 241 TLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEKV 300

Query: 301 KPYYVSLINKYFPEKLRW 318
           KPYYVSLINKYFPEKLRW
Sbjct: 301 KPYYVSLINKYFPEKLRW 316

BLAST of Cp4.1LG18g03840 vs. NCBI nr
Match: XP_022987889.1 (inositol oxygenase 2-like [Cucurbita maxima] >XP_022987890.1 inositol oxygenase 2-like [Cucurbita maxima])

HSP 1 Score: 637 bits (1642), Expect = 2.68e-230
Identity = 309/318 (97.17%), Postives = 311/318 (97.80%), Query Frame = 0

Query: 1   MTILIEQPRFEVEEKKVGITELNELVLDGGFVVPNETAAAAAEEGFVALEMNAFGHSFRD 60
           MTILIEQPRFEVEEKK+GI E NELVLDGGFVVPN T AA  EEGFVALEMNAFGHSFRD
Sbjct: 1   MTILIEQPRFEVEEKKLGIIEPNELVLDGGFVVPNGTEAA--EEGFVALEMNAFGHSFRD 60

Query: 61  YEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDP 120
           YEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDP
Sbjct: 61  YEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDP 120

Query: 121 DLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPL 180
           DLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPL
Sbjct: 121 DLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPL 180

Query: 181 GCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGS 240
           GCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGS
Sbjct: 181 GCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGS 240

Query: 241 TLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEKV 300
           TLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKEL+DVEKV
Sbjct: 241 TLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVEKV 300

Query: 301 KPYYVSLINKYFPEKLRW 318
           KPYYVSLINKYFPE LRW
Sbjct: 301 KPYYVSLINKYFPENLRW 316

BLAST of Cp4.1LG18g03840 vs. NCBI nr
Match: XP_008450805.1 (PREDICTED: inositol oxygenase 2-like [Cucumis melo])

HSP 1 Score: 601 bits (1549), Expect = 3.88e-216
Identity = 291/319 (91.22%), Postives = 302/319 (94.67%), Query Frame = 0

Query: 1   MTILIEQPRFEVE-EKKVGITELNELVLDGGFVVPNETAAAAAEEGFVALEMNAFGHSFR 60
           MTILIEQP FEVE EKKVG+TEL ELVLDGGFVVPN    A   EGF A EMNAFGHSFR
Sbjct: 1   MTILIEQPHFEVEDEKKVGMTELKELVLDGGFVVPN----ARTTEGFAAPEMNAFGHSFR 60

Query: 61  DYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSD 120
           DYEAESERQKGVEEFY++NHIHQTY++V KMRE YKKMDRVEMSIWECCELLNDVVDDSD
Sbjct: 61  DYEAESERQKGVEEFYRKNHIHQTYEFVTKMREEYKKMDRVEMSIWECCELLNDVVDDSD 120

Query: 121 PDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHP 180
           PDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHP
Sbjct: 121 PDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHP 180

Query: 181 LGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENG 240
           +GCAFD SIVHHKYFKENPD ENP Y+TKNGIY+EGCGL+NVMISWGHDDYMYLVAKENG
Sbjct: 181 VGCAFDESIVHHKYFKENPDWENPTYSTKNGIYSEGCGLNNVMISWGHDDYMYLVAKENG 240

Query: 241 STLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEK 300
           STLPSAGLFIIRYHSFYPLH+AGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEK
Sbjct: 241 STLPSAGLFIIRYHSFYPLHRAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEK 300

Query: 301 VKPYYVSLINKYFPEKLRW 318
           VKPYY+SLINKYFPEKLRW
Sbjct: 301 VKPYYISLINKYFPEKLRW 315

BLAST of Cp4.1LG18g03840 vs. NCBI nr
Match: XP_011659961.1 (inositol oxygenase 2 [Cucumis sativus] >KGN66197.1 hypothetical protein Csa_007517 [Cucumis sativus])

HSP 1 Score: 598 bits (1543), Expect = 3.07e-215
Identity = 291/319 (91.22%), Postives = 300/319 (94.04%), Query Frame = 0

Query: 1   MTILIEQPRFEVE-EKKVGITELNELVLDGGFVVPNETAAAAAEEGFVALEMNAFGHSFR 60
           MTILIEQP FE E EKKVGITEL ELVLDGGFVVPN     A  EGF A EMNAFGHSFR
Sbjct: 1   MTILIEQPHFEGEDEKKVGITELKELVLDGGFVVPN-----AKTEGFAAPEMNAFGHSFR 60

Query: 61  DYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSD 120
           DYEAESERQKGVEEFY+QNHIHQTY++V+KMRE YKKMDR EMSIWECCELLNDVVDDSD
Sbjct: 61  DYEAESERQKGVEEFYRQNHIHQTYEFVEKMREEYKKMDREEMSIWECCELLNDVVDDSD 120

Query: 121 PDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHP 180
           PDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHP
Sbjct: 121 PDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHP 180

Query: 181 LGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENG 240
           +GCAFD SIVHHKYFKENPD ENP Y+TKNGIY+EGCGLDNV ISWGHDDYMYLVAKENG
Sbjct: 181 VGCAFDESIVHHKYFKENPDWENPTYSTKNGIYSEGCGLDNVKISWGHDDYMYLVAKENG 240

Query: 241 STLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEK 300
           STLPSAGLFIIRYHSFYPLH+AGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEK
Sbjct: 241 STLPSAGLFIIRYHSFYPLHRAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEK 300

Query: 301 VKPYYVSLINKYFPEKLRW 318
           VKPYY+SLINKYFPEKLRW
Sbjct: 301 VKPYYISLINKYFPEKLRW 314

BLAST of Cp4.1LG18g03840 vs. ExPASy TrEMBL
Match: A0A6J1HBL6 (Inositol oxygenase OS=Cucurbita moschata OX=3662 GN=LOC111461362 PE=3 SV=1)

HSP 1 Score: 650 bits (1676), Expect = 8.51e-236
Identity = 316/318 (99.37%), Postives = 316/318 (99.37%), Query Frame = 0

Query: 1   MTILIEQPRFEVEEKKVGITELNELVLDGGFVVPNETAAAAAEEGFVALEMNAFGHSFRD 60
           MTILIEQPRFEVEEKKVGITELNELVLDGGFVVPNETAAA  EEGFVALEMNAFGHSFRD
Sbjct: 1   MTILIEQPRFEVEEKKVGITELNELVLDGGFVVPNETAAA--EEGFVALEMNAFGHSFRD 60

Query: 61  YEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDP 120
           YEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDP
Sbjct: 61  YEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDP 120

Query: 121 DLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPL 180
           DLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPL
Sbjct: 121 DLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPL 180

Query: 181 GCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGS 240
           GCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGS
Sbjct: 181 GCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGS 240

Query: 241 TLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEKV 300
           TLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEKV
Sbjct: 241 TLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEKV 300

Query: 301 KPYYVSLINKYFPEKLRW 318
           KPYYVSLINKYFPEKLRW
Sbjct: 301 KPYYVSLINKYFPEKLRW 316

BLAST of Cp4.1LG18g03840 vs. ExPASy TrEMBL
Match: A0A6J1JI24 (Inositol oxygenase OS=Cucurbita maxima OX=3661 GN=LOC111485296 PE=3 SV=1)

HSP 1 Score: 637 bits (1642), Expect = 1.30e-230
Identity = 309/318 (97.17%), Postives = 311/318 (97.80%), Query Frame = 0

Query: 1   MTILIEQPRFEVEEKKVGITELNELVLDGGFVVPNETAAAAAEEGFVALEMNAFGHSFRD 60
           MTILIEQPRFEVEEKK+GI E NELVLDGGFVVPN T AA  EEGFVALEMNAFGHSFRD
Sbjct: 1   MTILIEQPRFEVEEKKLGIIEPNELVLDGGFVVPNGTEAA--EEGFVALEMNAFGHSFRD 60

Query: 61  YEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDP 120
           YEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDP
Sbjct: 61  YEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSDP 120

Query: 121 DLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPL 180
           DLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPL
Sbjct: 121 DLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHPL 180

Query: 181 GCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGS 240
           GCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGS
Sbjct: 181 GCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENGS 240

Query: 241 TLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEKV 300
           TLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKEL+DVEKV
Sbjct: 241 TLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELVDVEKV 300

Query: 301 KPYYVSLINKYFPEKLRW 318
           KPYYVSLINKYFPE LRW
Sbjct: 301 KPYYVSLINKYFPENLRW 316

BLAST of Cp4.1LG18g03840 vs. ExPASy TrEMBL
Match: A0A1S3BQR6 (Inositol oxygenase OS=Cucumis melo OX=3656 GN=LOC103492281 PE=3 SV=1)

HSP 1 Score: 601 bits (1549), Expect = 1.88e-216
Identity = 291/319 (91.22%), Postives = 302/319 (94.67%), Query Frame = 0

Query: 1   MTILIEQPRFEVE-EKKVGITELNELVLDGGFVVPNETAAAAAEEGFVALEMNAFGHSFR 60
           MTILIEQP FEVE EKKVG+TEL ELVLDGGFVVPN    A   EGF A EMNAFGHSFR
Sbjct: 1   MTILIEQPHFEVEDEKKVGMTELKELVLDGGFVVPN----ARTTEGFAAPEMNAFGHSFR 60

Query: 61  DYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSD 120
           DYEAESERQKGVEEFY++NHIHQTY++V KMRE YKKMDRVEMSIWECCELLNDVVDDSD
Sbjct: 61  DYEAESERQKGVEEFYRKNHIHQTYEFVTKMREEYKKMDRVEMSIWECCELLNDVVDDSD 120

Query: 121 PDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHP 180
           PDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHP
Sbjct: 121 PDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHP 180

Query: 181 LGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENG 240
           +GCAFD SIVHHKYFKENPD ENP Y+TKNGIY+EGCGL+NVMISWGHDDYMYLVAKENG
Sbjct: 181 VGCAFDESIVHHKYFKENPDWENPTYSTKNGIYSEGCGLNNVMISWGHDDYMYLVAKENG 240

Query: 241 STLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEK 300
           STLPSAGLFIIRYHSFYPLH+AGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEK
Sbjct: 241 STLPSAGLFIIRYHSFYPLHRAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEK 300

Query: 301 VKPYYVSLINKYFPEKLRW 318
           VKPYY+SLINKYFPEKLRW
Sbjct: 301 VKPYYISLINKYFPEKLRW 315

BLAST of Cp4.1LG18g03840 vs. ExPASy TrEMBL
Match: A0A0A0LZA8 (Inositol oxygenase OS=Cucumis sativus OX=3659 GN=Csa_1G575130 PE=3 SV=1)

HSP 1 Score: 598 bits (1543), Expect = 1.49e-215
Identity = 291/319 (91.22%), Postives = 300/319 (94.04%), Query Frame = 0

Query: 1   MTILIEQPRFEVE-EKKVGITELNELVLDGGFVVPNETAAAAAEEGFVALEMNAFGHSFR 60
           MTILIEQP FE E EKKVGITEL ELVLDGGFVVPN     A  EGF A EMNAFGHSFR
Sbjct: 1   MTILIEQPHFEGEDEKKVGITELKELVLDGGFVVPN-----AKTEGFAAPEMNAFGHSFR 60

Query: 61  DYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSD 120
           DYEAESERQKGVEEFY+QNHIHQTY++V+KMRE YKKMDR EMSIWECCELLNDVVDDSD
Sbjct: 61  DYEAESERQKGVEEFYRQNHIHQTYEFVEKMREEYKKMDREEMSIWECCELLNDVVDDSD 120

Query: 121 PDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHP 180
           PDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHP
Sbjct: 121 PDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHP 180

Query: 181 LGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENG 240
           +GCAFD SIVHHKYFKENPD ENP Y+TKNGIY+EGCGLDNV ISWGHDDYMYLVAKENG
Sbjct: 181 VGCAFDESIVHHKYFKENPDWENPTYSTKNGIYSEGCGLDNVKISWGHDDYMYLVAKENG 240

Query: 241 STLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEK 300
           STLPSAGLFIIRYHSFYPLH+AGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEK
Sbjct: 241 STLPSAGLFIIRYHSFYPLHRAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEK 300

Query: 301 VKPYYVSLINKYFPEKLRW 318
           VKPYY+SLINKYFPEKLRW
Sbjct: 301 VKPYYISLINKYFPEKLRW 314

BLAST of Cp4.1LG18g03840 vs. ExPASy TrEMBL
Match: A0A6J1CSQ8 (Inositol oxygenase OS=Momordica charantia OX=3673 GN=LOC111014416 PE=3 SV=1)

HSP 1 Score: 590 bits (1522), Expect = 2.95e-212
Identity = 286/320 (89.38%), Postives = 300/320 (93.75%), Query Frame = 0

Query: 1   MTILIEQPRF-EVEEKKVGITELNELVLDGGFVVP-NETAAAAAEEGFVALEMNAFGHSF 60
           MTILI+ P   EVEEKK  I+E+ EL LDGGFV+P N+TAAAAA EGFVA E+N+FG SF
Sbjct: 1   MTILIDHPHLIEVEEKKGAISEMKELALDGGFVIPDNQTAAAAAAEGFVAPEINSFGQSF 60

Query: 61  RDYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDS 120
           RDY AES+RQK VEEFY+ NHIHQTYD+VKKMRE Y KMDRVEMSIWECCELLNDVVDDS
Sbjct: 61  RDYAAESDRQKSVEEFYRMNHIHQTYDFVKKMREQYGKMDRVEMSIWECCELLNDVVDDS 120

Query: 121 DPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180
           DPDLDEPQIQHLLQTAEAIRKDYPD+DWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH
Sbjct: 121 DPDLDEPQIQHLLQTAEAIRKDYPDQDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTH 180

Query: 181 PLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKEN 240
           PLGCAFD SIVHHKYFKEN DSENPAYNTKNGIY+EGCGLDNVMISWGHDDYMYLVAKEN
Sbjct: 181 PLGCAFDESIVHHKYFKENSDSENPAYNTKNGIYSEGCGLDNVMISWGHDDYMYLVAKEN 240

Query: 241 GSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVE 300
           GSTLPSAGLFIIRYHSFYPLHK+GAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKE IDVE
Sbjct: 241 GSTLPSAGLFIIRYHSFYPLHKSGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKERIDVE 300

Query: 301 KVKPYYVSLINKYFPEKLRW 318
           KVKPYY+SLINKYFPEKLRW
Sbjct: 301 KVKPYYMSLINKYFPEKLRW 320

BLAST of Cp4.1LG18g03840 vs. TAIR 10
Match: AT2G19800.1 (myo-inositol oxygenase 2 )

HSP 1 Score: 515.8 bits (1327), Expect = 2.5e-146
Identity = 245/321 (76.32%), Postives = 276/321 (85.98%), Query Frame = 0

Query: 1   MTILIEQ--PRFEVEEKKVGITELNELVLDGGFVVPNETAAAAAEEGFVALEMNAFGHSF 60
           MTIL+E   P   V+EKKV     NELVLDGGFVVP     +   + F A +MN  GHSF
Sbjct: 1   MTILVEHFVPDSRVDEKKVIEERDNELVLDGGFVVPK----SKETDAFDAPDMNFLGHSF 60

Query: 61  RDYE-AESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDD 120
           RDYE  ESERQ+GVEEFY+  HIHQTYD+VKKMR+ Y K++++EMSIWECCELLN+VVD+
Sbjct: 61  RDYENGESERQQGVEEFYRMQHIHQTYDFVKKMRKEYGKLNKMEMSIWECCELLNNVVDE 120

Query: 121 SDPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDT 180
           SDPDLDEPQIQHLLQTAEAIR+DYPDEDWLHLTALIHDLGKVLLLP FGGLPQWAVVGDT
Sbjct: 121 SDPDLDEPQIQHLLQTAEAIRRDYPDEDWLHLTALIHDLGKVLLLPEFGGLPQWAVVGDT 180

Query: 181 HPLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKE 240
            P+GC FDS+ +HHKYFK N D  NP YNTKNG+YTEGCGLDNV++SWGHDDYMYLVAK+
Sbjct: 181 FPVGCTFDSANIHHKYFKGNHDINNPKYNTKNGVYTEGCGLDNVLMSWGHDDYMYLVAKK 240

Query: 241 NGSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDV 300
           NG+TLP AGLFIIRYHSFYPLHKAGAY HLMN+ED  +LKWLH+FNKYDLYSKSK L+DV
Sbjct: 241 NGTTLPHAGLFIIRYHSFYPLHKAGAYTHLMNDEDRDDLKWLHVFNKYDLYSKSKVLVDV 300

Query: 301 EKVKPYYVSLINKYFPEKLRW 319
           E+VKPYY+SLINKYFP KL+W
Sbjct: 301 EQVKPYYISLINKYFPAKLKW 317

BLAST of Cp4.1LG18g03840 vs. TAIR 10
Match: AT4G26260.2 (myo-inositol oxygenase 4 )

HSP 1 Score: 500.0 bits (1286), Expect = 1.4e-141
Identity = 241/321 (75.08%), Postives = 268/321 (83.49%), Query Frame = 0

Query: 1   MTILIEQPRFEVEE---KKVGITELNELVLDGGFVVPNETAAAAAEEGFVALEMNAFGHS 60
           MTI +E+P FE E    +K G   + EL LDGGF +P        +E F+A EMNAFG  
Sbjct: 1   MTISVEKPIFEEEVSAFEKSG-DNIGELKLDGGFSMPK--MDTNDDEAFLAPEMNAFGRQ 60

Query: 61  FRDYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDD 120
           FRDY+ ESERQKGVEEFY+  HI+QT D+VKKMR  Y K+D++ MSIWECCELLN+VVD+
Sbjct: 61  FRDYDVESERQKGVEEFYRLQHINQTVDFVKKMRAEYGKLDKMVMSIWECCELLNEVVDE 120

Query: 121 SDPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDT 180
           SDPDLDEPQIQHLLQ+AEAIRKDYP+EDWLHLTALIHDLGKV+ LP FGGLPQWAVVGDT
Sbjct: 121 SDPDLDEPQIQHLLQSAEAIRKDYPNEDWLHLTALIHDLGKVITLPQFGGLPQWAVVGDT 180

Query: 181 HPLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKE 240
            P+GCAFD S VHHKYF ENPD  N  YNTKNGIY+EGCGL+NVM+SWGHDDYMYLVAKE
Sbjct: 181 FPVGCAFDESNVHHKYFVENPDFHNETYNTKNGIYSEGCGLNNVMMSWGHDDYMYLVAKE 240

Query: 241 NGSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDV 300
           NGSTLPSAG FIIRYHSFYPLH AG Y HLMNEED +NLKWLH+FNKYDLYSKSK  +DV
Sbjct: 241 NGSTLPSAGQFIIRYHSFYPLHTAGEYTHLMNEEDKENLKWLHVFNKYDLYSKSKVHVDV 300

Query: 301 EKVKPYYVSLINKYFPEKLRW 319
           EKVKPYY+SLI KYFPE LRW
Sbjct: 301 EKVKPYYMSLIKKYFPENLRW 318

BLAST of Cp4.1LG18g03840 vs. TAIR 10
Match: AT4G26260.1 (myo-inositol oxygenase 4 )

HSP 1 Score: 499.2 bits (1284), Expect = 2.4e-141
Identity = 239/319 (74.92%), Postives = 266/319 (83.39%), Query Frame = 0

Query: 1   MTILIEQPRF-EVEEKKVGITELNELVLDGGFVVPNETAAAAAEEGFVALEMNAFGHSFR 60
           MTI +E+P F EV   +     + EL LDGGF +P        +E F+A EMNAFG  FR
Sbjct: 1   MTISVEKPIFEEVSAFEKSGDNIGELKLDGGFSMPK--MDTNDDEAFLAPEMNAFGRQFR 60

Query: 61  DYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDVVDDSD 120
           DY+ ESERQKGVEEFY+  HI+QT D+VKKMR  Y K+D++ MSIWECCELLN+VVD+SD
Sbjct: 61  DYDVESERQKGVEEFYRLQHINQTVDFVKKMRAEYGKLDKMVMSIWECCELLNEVVDESD 120

Query: 121 PDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVVGDTHP 180
           PDLDEPQIQHLLQ+AEAIRKDYP+EDWLHLTALIHDLGKV+ LP FGGLPQWAVVGDT P
Sbjct: 121 PDLDEPQIQHLLQSAEAIRKDYPNEDWLHLTALIHDLGKVITLPQFGGLPQWAVVGDTFP 180

Query: 181 LGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLVAKENG 240
           +GCAFD S VHHKYF ENPD  N  YNTKNGIY+EGCGL+NVM+SWGHDDYMYLVAKENG
Sbjct: 181 VGCAFDESNVHHKYFVENPDFHNETYNTKNGIYSEGCGLNNVMMSWGHDDYMYLVAKENG 240

Query: 241 STLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKELIDVEK 300
           STLPSAG FIIRYHSFYPLH AG Y HLMNEED +NLKWLH+FNKYDLYSKSK  +DVEK
Sbjct: 241 STLPSAGQFIIRYHSFYPLHTAGEYTHLMNEEDKENLKWLHVFNKYDLYSKSKVHVDVEK 300

Query: 301 VKPYYVSLINKYFPEKLRW 319
           VKPYY+SLI KYFPE LRW
Sbjct: 301 VKPYYMSLIKKYFPENLRW 317

BLAST of Cp4.1LG18g03840 vs. TAIR 10
Match: AT1G14520.2 (myo-inositol oxygenase 1 )

HSP 1 Score: 476.1 bits (1224), Expect = 2.2e-134
Identity = 230/324 (70.99%), Postives = 264/324 (81.48%), Query Frame = 0

Query: 1   MTILI----EQPRFEVEEKKV--GITELNELVLDGGFVVPNETAAAAAEEGFVALEMNAF 60
           MTILI    +Q   E+ EK    G  E  ELVLD GF  P+                N+F
Sbjct: 1   MTILIDRHSDQNGDEIVEKNQGNGKEEETELVLDAGFEAPH---------------TNSF 60

Query: 61  GHSFRDYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLNDV 120
           G +FRDY+AESER++GVEEFY+ NHI QT D+V+KMRE Y+K++R EMSIWECCELLN+ 
Sbjct: 61  GRTFRDYDAESERRRGVEEFYRVNHIGQTVDFVRKMREEYEKLNRTEMSIWECCELLNEF 120

Query: 121 VDDSDPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWAVV 180
           +D+SDPDLDEPQI+HLLQTAEAIRKDYPDEDWLHLT LIHDLGKVLL  SFG LPQWAVV
Sbjct: 121 IDESDPDLDEPQIEHLLQTAEAIRKDYPDEDWLHLTGLIHDLGKVLLHSSFGELPQWAVV 180

Query: 181 GDTHPLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMYLV 240
           GDT P+GCAFD SIVHHKYFKENPD +NP+YN+K GIYTEGCGLDNV++SWGHDDYMYLV
Sbjct: 181 GDTFPVGCAFDESIVHHKYFKENPDYDNPSYNSKYGIYTEGCGLDNVLMSWGHDDYMYLV 240

Query: 241 AKENGSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSKEL 300
           AKEN +TLPSAGLFIIRYHSFY LHK+ AYKHLMN ED +N+KWL +FNKYDLYSKSK  
Sbjct: 241 AKENQTTLPSAGLFIIRYHSFYALHKSEAYKHLMNNEDRENMKWLKVFNKYDLYSKSKVR 300

Query: 301 IDVEKVKPYYVSLINKYFPEKLRW 319
           ++VE+VKPYY+SL NKYFP KL+W
Sbjct: 301 VNVEEVKPYYLSLTNKYFPSKLKW 309

BLAST of Cp4.1LG18g03840 vs. TAIR 10
Match: AT1G14520.1 (myo-inositol oxygenase 1 )

HSP 1 Score: 474.2 bits (1219), Expect = 8.4e-134
Identity = 229/326 (70.25%), Postives = 264/326 (80.98%), Query Frame = 0

Query: 1   MTILIEQ------PRFEVEEKKV--GITELNELVLDGGFVVPNETAAAAAEEGFVALEMN 60
           MTILI++         E+ EK    G  E  ELVLD GF  P+                N
Sbjct: 1   MTILIDRHSDQNDAGDEIVEKNQGNGKEEETELVLDAGFEAPH---------------TN 60

Query: 61  AFGHSFRDYEAESERQKGVEEFYKQNHIHQTYDYVKKMRETYKKMDRVEMSIWECCELLN 120
           +FG +FRDY+AESER++GVEEFY+ NHI QT D+V+KMRE Y+K++R EMSIWECCELLN
Sbjct: 61  SFGRTFRDYDAESERRRGVEEFYRVNHIGQTVDFVRKMREEYEKLNRTEMSIWECCELLN 120

Query: 121 DVVDDSDPDLDEPQIQHLLQTAEAIRKDYPDEDWLHLTALIHDLGKVLLLPSFGGLPQWA 180
           + +D+SDPDLDEPQI+HLLQTAEAIRKDYPDEDWLHLT LIHDLGKVLL  SFG LPQWA
Sbjct: 121 EFIDESDPDLDEPQIEHLLQTAEAIRKDYPDEDWLHLTGLIHDLGKVLLHSSFGELPQWA 180

Query: 181 VVGDTHPLGCAFDSSIVHHKYFKENPDSENPAYNTKNGIYTEGCGLDNVMISWGHDDYMY 240
           VVGDT P+GCAFD SIVHHKYFKENPD +NP+YN+K GIYTEGCGLDNV++SWGHDDYMY
Sbjct: 181 VVGDTFPVGCAFDESIVHHKYFKENPDYDNPSYNSKYGIYTEGCGLDNVLMSWGHDDYMY 240

Query: 241 LVAKENGSTLPSAGLFIIRYHSFYPLHKAGAYKHLMNEEDAQNLKWLHIFNKYDLYSKSK 300
           LVAKEN +TLPSAGLFIIRYHSFY LHK+ AYKHLMN ED +N+KWL +FNKYDLYSKSK
Sbjct: 241 LVAKENQTTLPSAGLFIIRYHSFYALHKSEAYKHLMNNEDRENMKWLKVFNKYDLYSKSK 300

Query: 301 ELIDVEKVKPYYVSLINKYFPEKLRW 319
             ++VE+VKPYY+SL NKYFP KL+W
Sbjct: 301 VRVNVEEVKPYYLSLTNKYFPSKLKW 311

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O822003.5e-14576.32Inositol oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=MIOX2 PE=2 SV=2[more]
Q8H1S03.4e-14074.92Inositol oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=MIOX4 PE=2 SV=1[more]
Q8L7991.2e-13270.25Inositol oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=MIOX1 PE=2 SV=1[more]
Q5Z8T35.9e-13270.90Probable inositol oxygenase OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g056... [more]
Q9FJU43.8e-13171.16Inositol oxygenase 5 OS=Arabidopsis thaliana OX=3702 GN=MIOX5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023515618.11.19e-238100.00inositol oxygenase 2-like [Cucurbita pepo subsp. pepo] >KAG7023630.1 Inositol ox... [more]
XP_022960634.11.76e-23599.37inositol oxygenase 2-like [Cucurbita moschata] >KAG6589967.1 Inositol oxygenase ... [more]
XP_022987889.12.68e-23097.17inositol oxygenase 2-like [Cucurbita maxima] >XP_022987890.1 inositol oxygenase ... [more]
XP_008450805.13.88e-21691.22PREDICTED: inositol oxygenase 2-like [Cucumis melo][more]
XP_011659961.13.07e-21591.22inositol oxygenase 2 [Cucumis sativus] >KGN66197.1 hypothetical protein Csa_0075... [more]
Match NameE-valueIdentityDescription
A0A6J1HBL68.51e-23699.37Inositol oxygenase OS=Cucurbita moschata OX=3662 GN=LOC111461362 PE=3 SV=1[more]
A0A6J1JI241.30e-23097.17Inositol oxygenase OS=Cucurbita maxima OX=3661 GN=LOC111485296 PE=3 SV=1[more]
A0A1S3BQR61.88e-21691.22Inositol oxygenase OS=Cucumis melo OX=3656 GN=LOC103492281 PE=3 SV=1[more]
A0A0A0LZA81.49e-21591.22Inositol oxygenase OS=Cucumis sativus OX=3659 GN=Csa_1G575130 PE=3 SV=1[more]
A0A6J1CSQ82.95e-21289.38Inositol oxygenase OS=Momordica charantia OX=3673 GN=LOC111014416 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G19800.12.5e-14676.32myo-inositol oxygenase 2 [more]
AT4G26260.21.4e-14175.08myo-inositol oxygenase 4 [more]
AT4G26260.12.4e-14174.92myo-inositol oxygenase 4 [more]
AT1G14520.22.2e-13470.99myo-inositol oxygenase 1 [more]
AT1G14520.18.4e-13470.25myo-inositol oxygenase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007828Inositol oxygenasePFAMPF05153MIOXcoord: 69..318
e-value: 4.3E-125
score: 416.3
IPR007828Inositol oxygenasePANTHERPTHR12588MYOINOSITOL OXYGENASEcoord: 1..318
NoneNo IPR availablePANTHERPTHR12588:SF14INOSITOL OXYGENASE 5coord: 1..318
NoneNo IPR availableSUPERFAMILY109604HD-domain/PDEase-likecoord: 62..318

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG18g03840.1Cp4.1LG18g03840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019310 inositol catabolic process
biological_process GO:0019853 L-ascorbic acid biosynthetic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0050113 inositol oxygenase activity
molecular_function GO:0005506 iron ion binding