Cp4.1LG18g03290 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG18g03290 vs. ExPASy Swiss-Prot
Match: Q9LYG9 (Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10 PE=1 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 2.1e-199
Identity = 390/733 (53.21%), Postives = 520/733 (70.94%), Query Frame = 0

Query: 32  VASVPDFEPKETRSVRCAIPQS----VVESSPSHEISRM--SSLKPPKIP----VSAVRR 91
           V +VP  E    RS   A P+S        SPS EIS++  S  KPP+ P    V   +R
Sbjct: 17  VINVP-VEEASRRSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQR 76

Query: 92  PSFARSSFSKPKSRLIEPPCPDGASLTEENAQAKSASGSPFSSSPKRDSPANIATATSPK 151
            SFARS +SKPKSR ++P CP   S+ EE  + +  +G  FS    R SP N     S +
Sbjct: 77  KSFARSVYSKPKSRFVDPSCPVDTSILEEEVREQLGAGFSFS----RASPNN----KSNR 136

Query: 152 ESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKEKSRKKLKRAVLIEWIAFLCITA 211
                AP+TP   +V     E+++DEE+YK  ++K+  + R K+    LIE   F+ I +
Sbjct: 137 SVGSPAPVTPSKVVV-----EKDEDEEIYK--KVKLNREMRSKISTLALIESAFFVVILS 196

Query: 212 CLISSLTIDKLLTKEIWGLELWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVL 271
            L++SLTI+ L     WGLE+WKWCVLV+VIF G L + WF+  +VFLIE NFLL+RKVL
Sbjct: 197 ALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVL 256

Query: 272 YFVYGLRKSVIVLIWLALVLLAWGLLFDQSIKRSKKVNEILNYVTRALAASLIGAGLWLV 331
           YFV+GL+KSV V IWL L+L+AW LLF+  +KRS    ++L  +TR L + L GA  WLV
Sbjct: 257 YFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLV 316

Query: 332 KTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLMEMAERVGRVASSGQLSFRHL 391
           KTLL+KILAA+F    FFDRIQ+S+FHQY+L+TLSG PLME AERVGR  S+G LSF  +
Sbjct: 317 KTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATV 376

Query: 392 KKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLINVIRSSGLSTISNTIENFKEEEG 451
            K+   G   +++VID+ K+ KMK+EKVSAWTMR L+  +R+SGLSTIS+T++     EG
Sbjct: 377 VKK---GTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEG 436

Query: 452 -EQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLFRFMNKEEIDNVLPLFEGGVET 511
            EQ D+EI SE EA AAAY +FRNVA+P   YI+EEDL RFM KEE+D V PLF+G  ET
Sbjct: 437 KEQADREITSEMEALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAET 496

Query: 512 GKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRLASAFVLIVIVIVWLLLMGFLT 571
           G+I RK    W+V VY  R++LAHSLNDTKTA+++LN+L +A +++V V++WLLL+   T
Sbjct: 497 GRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVAT 556

Query: 572 TQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFDVGDRCVVDGVQMIVEEMNILT 631
           T+VL+F S+QL+ + F+ G+T K +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LT
Sbjct: 557 TKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLT 616

Query: 632 TIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFSVDFSTSIESIGALKARIKSYL 691
           T+FL+ +NEK++YPN+VLATKPISN++RSP M ++++FS+ FST +  I  LK RI  YL
Sbjct: 617 TVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYL 676

Query: 692 ESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNYGDRSSRRSDLVLELKKIFEDL 751
           E  PQ W P HSV+VKEIEN+NK+K++LY +HTI FQ   +R+ RR++L L +K++ EDL
Sbjct: 677 EQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDL 730

Query: 752 GIKYHLLPQEVQL 754
            I Y LLPQ++ L
Sbjct: 737 HIDYTLLPQDINL 730

BLAST of Cp4.1LG18g03290 vs. ExPASy Swiss-Prot
Match: Q84M97 (Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 PE=1 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 3.8e-177
Identity = 360/759 (47.43%), Postives = 499/759 (65.74%), Query Frame = 0

Query: 8   EQVVLRILDGEEGVDARNDLTNGSVASVPDFEPKETRSVRCAIPQSVVESSPSHEISRMS 67
           E+VV+ + D E+  D R   +   +AS     P     +  + P   + S P+ EI + S
Sbjct: 10  EEVVINVSDKEDSKDPRASPSFNPLAS-----PDSDAGIEKSKPVPPI-SIPTPEIYKFS 69

Query: 68  SL--KPPKI--PVSAVRRPSFARSSFSKPKSRLIEPPC---------PDGASLTEENAQA 127
               KPPKI  P   VRR S +RS +SKPKSR  E              G SL E+    
Sbjct: 70  GSVHKPPKIPSPEGLVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLREQFGAG 129

Query: 128 KSASGSPFSSSPKRDSPANIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAE 187
             A GS   +SP   S  ++A+A   K +                  E +++EE+YK  +
Sbjct: 130 SFARGSFDRASPNNKSNRSVASAALSKVA----------------EEEPDENEEIYK--K 189

Query: 188 LKVKEKSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLELWKWCVLVLVIFC 247
           +K+    R  +K    +E + F+ I   LI SLTID +    IWGLE WKWCVLV+V   
Sbjct: 190 VKLHRVKRSGMKPLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLS 249

Query: 248 GRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVLIWLALVLLAWGLLFDQSIKR 307
           G L + WF++ +VF+IE+N+LL++KVLYFV+GL+K+V V IW +LVL+AW  LFD  +KR
Sbjct: 250 GMLVTNWFMHFVVFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKR 309

Query: 308 SKKVNEILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRT 367
           ++K    L+++T  + + L+G+ L+LVKT  +K+LA+ F    FF+RIQES+FHQY+L+T
Sbjct: 310 TRKTKRFLDFITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQT 369

Query: 368 LSGPPLMEMAERVGRVASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTM 427
           LSGPPL+E AE VGRV S+G LSF   K     G    ++VID+ K+ +MKQEKVSAWTM
Sbjct: 370 LSGPPLIEEAENVGRVPSTGHLSFTRTK----DGKVKDKKVIDMGKVHRMKQEKVSAWTM 429

Query: 428 RGLINVIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYID 487
           R LI  + +SG+STIS+T++    ++ E+ DKEI +E EA AAAY +F NVAKP   YI+
Sbjct: 430 RVLIEAVGTSGISTISSTLDEVNNKK-ERTDKEITNEMEAVAAAYDVFNNVAKPNHNYIE 489

Query: 488 EEDLFRFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIE 547
           E+DL RFM KEE+D VLPL E   +TGKI RKT   W+VNVY  RK++ HSLNDTKTA++
Sbjct: 490 EDDLLRFMIKEEVDLVLPLIE-DADTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVK 549

Query: 548 ELNRLASAFVLIVIVIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFV 607
           +L++L +  + ++  IVW++L+   +T++L+  SSQ L + FM G+T K IFE+ +FVFV
Sbjct: 550 QLDKLITGILTVITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFV 609

Query: 608 MHPFDVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSD 667
           MHP+DVGDRCVVDGV ++VEE+++LTT+FL+ DNEK+FYPNSVL +KPISNFYRSP+M D
Sbjct: 610 MHPYDVGDRCVVDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGD 669

Query: 668 SIDFSVDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTI 727
            +DF + FST  E IG LK +I  YL +  Q W P   V+V+ IEN+NK+ L++ + HTI
Sbjct: 670 YVDFGIAFSTPAEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTI 729

Query: 728 NFQNYGDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQL 754
           NFQ Y ++S RR+ L++ +K+I EDL I Y LLPQ+V L
Sbjct: 730 NFQVYVEKSLRRTALIIAIKRILEDLEIDYTLLPQDVNL 738

BLAST of Cp4.1LG18g03290 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 2.6e-149
Identity = 313/718 (43.59%), Postives = 452/718 (62.95%), Query Frame = 0

Query: 80  RRPSFARSSFSKPKSRLIEPPCPDGASLTEENAQAKSASG---SPF-SSSPKRDSPANIA 139
           ++P  +R   +K KSRL +PP P   ++ +   ++   SG   S F   SPK  +P    
Sbjct: 185 KKPMLSR---NKTKSRLQDPPTPTHPAIDKTEMKSGRRSGIFKSGFLGKSPKAGTP---- 244

Query: 140 TATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKEKSRKKLKRAVLIEWIA 199
                                G  G EEE++E+ +   +L  +E  R KL   V +EWI+
Sbjct: 245 ---------------------GRNGFEEEEEEDPFLDEDLP-EEFKRDKLSFWVFLEWIS 304

Query: 200 FLCITACLISSLTIDKLLTKEIWGLELWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFL 259
            + I   L+ SLTI  L  K  W L+LWKW V VLV+ CGRL S W +  +VFL+E+NF 
Sbjct: 305 LVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKNFT 364

Query: 260 LKRKVLYFVYGLRKSVIVLIWLALVLLAWGLLFDQSIKRSKKVNEILNYVTRALAASLIG 319
            +++VLYFVYG+RKSV   +WL LVLLAW  LFD+ ++R  + +  L YVTR L   L+ 
Sbjct: 365 WRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETR-STALRYVTRVLVCLLVA 424

Query: 320 AGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLME---MAERVGRVAS 379
             +WLVKT+LVK+LA+SF  + +FDRIQES+F QY++ TLSGPPLME   M E   +VA 
Sbjct: 425 LIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAE 484

Query: 380 SGQ------------------LSFRHLKK----ERDGGNEGKE-EVIDVDKLKKMKQEKV 439
             +                   SF  + K     R G   G++ E I +D+LK+M  + V
Sbjct: 485 DVKSLEKLAGAKLPPALKATVKSFMKVGKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNV 544

Query: 440 SAWTMRGLINVIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPG 499
           SAW M+ L+N+I    +ST+   +++  +E  ++    I SE+EA  AA +IF NV +PG
Sbjct: 545 SAWNMKRLMNIILKGAISTLDQNMQDTTQE--DEDATHIRSEYEAKCAARKIFHNVTEPG 604

Query: 500 SKYIDEEDLFRFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDT 559
           S+YI  ED  RF+ +EE +  + LFEG  E+ KI +  LKNW+V  + ER++LA +LNDT
Sbjct: 605 SRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVKAFRERRALALTLNDT 664

Query: 560 KTAIEELNRLASAFVLIVIVIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAI 619
           KTA++ L+R+ +  + I+I+I+WLL++G  TT+ L+ +SSQLLLV F+FGN+ KTIFEAI
Sbjct: 665 KTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVFGNSCKTIFEAI 724

Query: 620 IFVFVMHPFDVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRS 679
           IF+FVMHPFDVGDRC +DGVQ++VEEMNILTT+FLRYDN+KI YPNSVL TKPI+N+YRS
Sbjct: 725 IFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRS 784

Query: 680 PEMSDSIDFSVDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLY 739
           P+M D+++F V  +T  E I A+K RI SY+++K  +W P   ++   ++++N +K++++
Sbjct: 785 PDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVW 844

Query: 740 INHTINFQNYGDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSAVAPMVSTTS 768
           + H +N Q+ G+R  RR  L+ E+ K   +L I+Y L P  +    V ++ P  + TS
Sbjct: 845 LTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNIN---VRSLPPTANPTS 867

BLAST of Cp4.1LG18g03290 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 3.5e-146
Identity = 323/756 (42.72%), Postives = 464/756 (61.38%), Query Frame = 0

Query: 52  QSVVESSPSHEISRMSSLKPPKIPVSAVRRPSFARSSF-------SKPKSRLIEPPCPDG 111
           Q+ V  SPS +    S  +  ++  S V + +  RS+         K +SRL++PP P  
Sbjct: 139 QNRVTLSPSVKDGDSSEDEENRVDGSEVVKCTSNRSTMRTKTLMKMKTRSRLMDPPTPT- 198

Query: 112 ASLTEENAQAKSASGSPFSSSPKRDSPANIATATSPKESLKSAPITPRTPLVGSTGSEEE 171
                           P   S +     N+    S + +       P TP  G  GS++ 
Sbjct: 199 ---------------YPDMVSGRTPRSGNLNPGFSGRNT------KPGTPNQG--GSKDL 258

Query: 172 DDEEVYKTAELKVKEKSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLELWK 231
           ++EE   + E   +   ++K+   V+IEWI  + I A LI SL I  L  K +W L LWK
Sbjct: 259 EEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLILIIASLICSLVIPYLRGKTLWDLALWK 318

Query: 232 WCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVLIWLALVLLAW 291
           W V+VLV+ CGRL S W +   V+ +E NFL ++KVLYFVYG+RK V   +WL LVL+AW
Sbjct: 319 WEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQNCLWLGLVLIAW 378

Query: 292 GLLFDQSIKRSKKVNEILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQE 351
             LFD+ ++R  + + +L YVT+ L   L+   +WL+KTLLVK+LA+SF  + +FDRIQE
Sbjct: 379 HFLFDKKVEREMR-STVLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFHMSTYFDRIQE 438

Query: 352 SIFHQYILRTLSGPPLMEM-------------AERVGR---------VASSGQLSF--RH 411
           S+F QY++ TLSGPP +E+              E VGR         V+S  Q++     
Sbjct: 439 SLFTQYVIETLSGPPRIEIHIEEEKVANDVKTFEIVGRKLSPLGPKAVSSPPQVTVGSGR 498

Query: 412 LKK-----------ERDGG-NEGKEEVIDVDKLKKMKQEKVSAWTMRGLINVIRSSGLST 471
           L+K            R G   EG EE I +D L++M  + VSAW M+ L+NVI+   LST
Sbjct: 499 LQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIKKGTLST 558

Query: 472 ISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLFRFMNKEEID 531
           +   I++   +E + K  +I SE+EA  AA +IF+NVA+PGS+YI  ED  RF++++E +
Sbjct: 559 LDEQIQDTTTQE-DDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYMEDFMRFLSEDESE 618

Query: 532 NVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRLASAFVLIVI 591
             + LFEG  E  KI +  LKNW+VN + ER++LA +LNDTKTA+  L+R+    V IVI
Sbjct: 619 RAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNRLHRIVDVLVSIVI 678

Query: 592 VIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFDVGDRCVVDG 651
           +I+WLL++G  TT+ L+ ISSQLLLVVF+FGN+ KTIFEA+IFVFVMHPFDVGDRC +DG
Sbjct: 679 LIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDG 738

Query: 652 VQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFSVDFSTSIES 711
           VQMIVEEMNILTT+FLR+DN+KI YPNS+L TKPI+N+YRSP+M D+I+F V  +T  E 
Sbjct: 739 VQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDAIEFFVHIATPPEK 798

Query: 712 IGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNYGDRSSRRSD 765
             AL+ RI SY+++K   W P+  ++ +++  +N +K++++  H +N QN G+R  RR  
Sbjct: 799 TTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKMNHQNMGERYVRRGQ 858

BLAST of Cp4.1LG18g03290 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 8.9e-142
Identity = 290/689 (42.09%), Postives = 430/689 (62.41%), Query Frame = 0

Query: 91  KPKSRLIEPPCPDGASLTEENAQAKSASGSPFSSSPKRDSPANIATATSPKESLKSAPIT 150
           + +SRL +PP P    L  + A  KS         PK     +     SPK         
Sbjct: 172 RTRSRLSDPPTP---QLPPQTADMKS------GRIPKSGQMKSGFFGKSPK--------- 231

Query: 151 PRTPLVGSTGSEEEDDEEVYKTAELKVKEKSRKKLKRAVLIEWIAFLCITACLISSLTID 210
                  + G EEEDD      AE   +E  + KL   +++EW++ + I A  + +L I 
Sbjct: 232 -------TQGEEEEDDP---FAAEDLPEEYRKDKLSLWIVLEWLSLILIIAGFVCTLAIP 291

Query: 211 KLLTKEIWGLELWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLRKS 270
            L  K++W L+LWKW  +VLV+ CGRL S W +  +VF IERNFLL+++VLYFVYG+RK+
Sbjct: 292 SLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKA 351

Query: 271 VIVLIWLALVLLAWGLLFDQSIKRSKKVNEILNYVTRALAASLIGAGLWLVKTLLVKILA 330
           V   +WL LVLLAW  LFD+ + ++    + L  VT+     L+G  LWLVKTLLVK+LA
Sbjct: 352 VQNCLWLGLVLLAWHFLFDEKVAKAAN-TKALRVVTKIFVCLLVGFLLWLVKTLLVKVLA 411

Query: 331 ASFQCTRFFDRIQESIFHQYILRTLSGPPLMEM---AERVGRVA---------------- 390
           +SF  + +FDRIQES+F QY++ TLSGPPL+E+    E   R++                
Sbjct: 412 SSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVEVKKFQNPGGVEIQS 471

Query: 391 -------SSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLINVIRSS 450
                   +G+  F        GG  G+ + I +D L K+  + VSAW M+ L+N+IR+ 
Sbjct: 472 GAQKSPMKTGKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNG 531

Query: 451 GLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLFRFMNK 510
            L+T+   +++      + K  +I SE+EA  AA +IF NVAKPGSK+I   D+ RF+  
Sbjct: 532 SLTTLDEQLQD--PSLDDDKGNQIRSEFEAKLAARKIFHNVAKPGSKFIYANDIMRFLPD 591

Query: 511 EEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRLASAFV 570
           +E    L LFEG  ET +I + +LKNW+VN + ER++LA +LNDTKTA+  L+++ +  V
Sbjct: 592 DEALKTLSLFEGASETNRISKSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVV 651

Query: 571 LIVIVIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFDVGDRC 630
            I+I+++WL+++G  +T+ LV +SSQ+++V F+FGN  K +FE+II++FV+HPFDVGDRC
Sbjct: 652 GIIILVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRC 711

Query: 631 VVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFSVDFST 690
            +DGVQM+VEEMNILTT+FLR+DN+K+ YPNS+L TK I N+YRSP+M D I+FS+  +T
Sbjct: 712 EIDGVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITT 771

Query: 691 SIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNYGDRSS 750
             E I  +K RI SY+E K   W P   ++ K++E++N ++++++  H +N Q+ G++ +
Sbjct: 772 PAEKIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWA 829

Query: 751 RRSDLVLELKKIFEDLGIKYHLLPQEVQL 754
           RRS LV E+ KI  +L I+Y L P ++ +
Sbjct: 832 RRSQLVEEIAKICRELDIEYRLYPLDINV 829

BLAST of Cp4.1LG18g03290 vs. NCBI nr
Match: XP_023515637.1 (mechanosensitive ion channel protein 10-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1449 bits (3752), Expect = 0.0
Identity = 769/769 (100.00%), Postives = 769/769 (100.00%), Query Frame = 0

Query: 1   MADKKGTEQVVLRILDGEEGVDARNDLTNGSVASVPDFEPKETRSVRCAIPQSVVESSPS 60
           MADKKGTEQVVLRILDGEEGVDARNDLTNGSVASVPDFEPKETRSVRCAIPQSVVESSPS
Sbjct: 1   MADKKGTEQVVLRILDGEEGVDARNDLTNGSVASVPDFEPKETRSVRCAIPQSVVESSPS 60

Query: 61  HEISRMSSLKPPKIPVSAVRRPSFARSSFSKPKSRLIEPPCPDGASLTEENAQAKSASGS 120
           HEISRMSSLKPPKIPVSAVRRPSFARSSFSKPKSRLIEPPCPDGASLTEENAQAKSASGS
Sbjct: 61  HEISRMSSLKPPKIPVSAVRRPSFARSSFSKPKSRLIEPPCPDGASLTEENAQAKSASGS 120

Query: 121 PFSSSPKRDSPANIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKEK 180
           PFSSSPKRDSPANIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKEK
Sbjct: 121 PFSSSPKRDSPANIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKEK 180

Query: 181 SRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLELWKWCVLVLVIFCGRLFSQ 240
           SRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLELWKWCVLVLVIFCGRLFSQ
Sbjct: 181 SRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLELWKWCVLVLVIFCGRLFSQ 240

Query: 241 WFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVLIWLALVLLAWGLLFDQSIKRSKKVNE 300
           WFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVLIWLALVLLAWGLLFDQSIKRSKKVNE
Sbjct: 241 WFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVLIWLALVLLAWGLLFDQSIKRSKKVNE 300

Query: 301 ILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPL 360
           ILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPL
Sbjct: 301 ILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPL 360

Query: 361 MEMAERVGRVASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLINV 420
           MEMAERVGRVASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLINV
Sbjct: 361 MEMAERVGRVASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLINV 420

Query: 421 IRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLFR 480
           IRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLFR
Sbjct: 421 IRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLFR 480

Query: 481 FMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRLA 540
           FMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRLA
Sbjct: 481 FMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRLA 540

Query: 541 SAFVLIVIVIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFDV 600
           SAFVLIVIVIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFDV
Sbjct: 541 SAFVLIVIVIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFDV 600

Query: 601 GDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFSV 660
           GDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFSV
Sbjct: 601 GDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFSV 660

Query: 661 DFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNYG 720
           DFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNYG
Sbjct: 661 DFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNYG 720

Query: 721 DRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSAVAPMVSTTSRR 769
           DRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSAVAPMVSTTSRR
Sbjct: 721 DRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSAVAPMVSTTSRR 769

BLAST of Cp4.1LG18g03290 vs. NCBI nr
Match: XP_022987285.1 (mechanosensitive ion channel protein 10-like [Cucurbita maxima] >XP_022987286.1 mechanosensitive ion channel protein 10-like [Cucurbita maxima])

HSP 1 Score: 1413 bits (3657), Expect = 0.0
Identity = 752/769 (97.79%), Postives = 756/769 (98.31%), Query Frame = 0

Query: 1   MADKKGTEQVVLRILDGEEGVDARNDLTNGSVASVPDFEPKETRSVRCAIPQSVVESSPS 60
           MADKKGTEQVVLRILDGEEGVDARN LTNGSVAS PDFE KETRSVRCAIPQSVVESSPS
Sbjct: 1   MADKKGTEQVVLRILDGEEGVDARNGLTNGSVASFPDFESKETRSVRCAIPQSVVESSPS 60

Query: 61  HEISRMSSLKPPKIPV-SAVRRPSFARSSFSKPKSRLIEPPCPDGASLTEENAQAKSASG 120
           HEISRMSSLKPPKIPV SAVRRPSFARSSFSKPKSRLIEPPCPDGAS  EENAQAKS SG
Sbjct: 61  HEISRMSSLKPPKIPVESAVRRPSFARSSFSKPKSRLIEPPCPDGASSAEENAQAKSTSG 120

Query: 121 SPFSSSPKRDSPANIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180
           SPFSSSPK DSPA IATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE
Sbjct: 121 SPFSSSPKMDSPAKIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLELWKWCVLVLVIFCGRLFS 240
           KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGL LWKWCVLVLVIFCGRLFS
Sbjct: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240

Query: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVLIWLALVLLAWGLLFDQSIKRSKKVN 300
           QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIV IWLALVLLAWGLLFDQSIKRSKKVN
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360
           EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILR LSGPP
Sbjct: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPP 360

Query: 361 LMEMAERVGRVASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLIN 420
           LMEMAERVGRVAS+GQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQ KVSAWTMRGLIN
Sbjct: 361 LMEMAERVGRVASAGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQAKVSAWTMRGLIN 420

Query: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480
           VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF
Sbjct: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480

Query: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540
           RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL
Sbjct: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540

Query: 541 ASAFVLIVIVIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600
           ASAFVLIVI+IVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD
Sbjct: 541 ASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660
           VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS
Sbjct: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660

Query: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720
           VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY
Sbjct: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720

Query: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSAVAPMVSTTSR 768
           GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVS++APMVSTTSR
Sbjct: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSIAPMVSTTSR 769

BLAST of Cp4.1LG18g03290 vs. NCBI nr
Match: KAG6590025.1 (Mechanosensitive ion channel protein 10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1410 bits (3651), Expect = 0.0
Identity = 757/788 (96.07%), Postives = 760/788 (96.45%), Query Frame = 0

Query: 1   MADKKGTEQVVLRILDGEEGVDARNDLTNGSVASVPDFEPKETRSVRCAIPQSVVESSPS 60
           MADKKGTEQVVLRILDGEEGVDARNDLTNGSVASVPDFEPKETR VRCAIPQSVVESSPS
Sbjct: 1   MADKKGTEQVVLRILDGEEGVDARNDLTNGSVASVPDFEPKETRIVRCAIPQSVVESSPS 60

Query: 61  HEISRMSSLKPPKIPV-SAVRRPSFARSSFSKPKSRLIEPPCPDGASLTEENAQAKSASG 120
           HEISRMSSLKPPKIPV SAVRRPSFARSSFSKPKSRLIEPPCPDGASL EENAQAKSASG
Sbjct: 61  HEISRMSSLKPPKIPVGSAVRRPSFARSSFSKPKSRLIEPPCPDGASLAEENAQAKSASG 120

Query: 121 SPFSSSPKRDSPANIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180
           SPFSSSPK DS ANIATATSPKE+LKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE
Sbjct: 121 SPFSSSPKMDSTANIATATSPKEALKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLELWKWCVLVLVIFCGRLFS 240
           KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGL LWKWCVLVLVIFCGRLFS
Sbjct: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240

Query: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVLIWLALVLLAWGLLFDQSIKRSKKVN 300
           QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIV IWLALVLLAWGLLFDQSIKRSKKVN
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360
           EIL YVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP
Sbjct: 301 EILTYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360

Query: 361 LMEMAERVGRVASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLIN 420
           LMEMAERVG  ASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLIN
Sbjct: 361 LMEMAERVGGAASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLIN 420

Query: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480
           VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF
Sbjct: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480

Query: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540
           RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL
Sbjct: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540

Query: 541 ASAFVLIVIVIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600
           ASAFVLIVI+IVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD
Sbjct: 541 ASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660
           VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS
Sbjct: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660

Query: 661 VDFSTSIESIGALKARIKS------------------YLESKPQFWRPNHSVIVKEIENV 720
           VDFSTSIESIGALKARIKS                  YLESKPQFWRPNHSVIVKEIENV
Sbjct: 661 VDFSTSIESIGALKARIKSSLSFQSTHLLDITELFLRYLESKPQFWRPNHSVIVKEIENV 720

Query: 721 NKMKLSLYINHTINFQNYGDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSAVAP 769
           NKMKLSLYINHTINFQNYGDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVS+VAP
Sbjct: 721 NKMKLSLYINHTINFQNYGDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSVAP 780

BLAST of Cp4.1LG18g03290 vs. NCBI nr
Match: KAG7023687.1 (Mechanosensitive ion channel protein 10, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1406 bits (3639), Expect = 0.0
Identity = 747/757 (98.68%), Postives = 749/757 (98.94%), Query Frame = 0

Query: 1   MADKKGTEQVVLRILDGEEGVDARNDLTNGSVASVPDFEPKETRSVRCAIPQSVVESSPS 60
           MADKKGTEQVVLRILDGEEGVDARNDLTNGSVASVPDFEPKETRSVRCAIPQSVVESSPS
Sbjct: 1   MADKKGTEQVVLRILDGEEGVDARNDLTNGSVASVPDFEPKETRSVRCAIPQSVVESSPS 60

Query: 61  HEISRMSSLKPPKIPV-SAVRRPSFARSSFSKPKSRLIEPPCPDGASLTEENAQAKSASG 120
           HEISRMSSLKPPKIPV SAVRRPSFARSSFSKPKSRLIEPPCPDGASL EENAQAKS SG
Sbjct: 61  HEISRMSSLKPPKIPVGSAVRRPSFARSSFSKPKSRLIEPPCPDGASLAEENAQAKSTSG 120

Query: 121 SPFSSSPKRDSPANIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180
           SPFSSSPK DSPANIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE
Sbjct: 121 SPFSSSPKMDSPANIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLELWKWCVLVLVIFCGRLFS 240
           KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGL LWKWCVLVLVIFCGRLFS
Sbjct: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240

Query: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVLIWLALVLLAWGLLFDQSIKRSKKVN 300
           QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIV IWLALVLLAWGLLFDQSIKRSKKVN
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360
           EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP
Sbjct: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360

Query: 361 LMEMAERVGRVASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLIN 420
           LMEMAERVG  ASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLIN
Sbjct: 361 LMEMAERVGGAASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLIN 420

Query: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480
           VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF
Sbjct: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480

Query: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540
           RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL
Sbjct: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540

Query: 541 ASAFVLIVIVIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600
           ASAFVLIVI+IVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD
Sbjct: 541 ASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660
           VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS
Sbjct: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660

Query: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720
           VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY
Sbjct: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720

Query: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYV 756
           GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNY+
Sbjct: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYL 757

BLAST of Cp4.1LG18g03290 vs. NCBI nr
Match: XP_022960737.1 (mechanosensitive ion channel protein 10-like [Cucurbita moschata] >XP_022960738.1 mechanosensitive ion channel protein 10-like [Cucurbita moschata])

HSP 1 Score: 1405 bits (3638), Expect = 0.0
Identity = 750/770 (97.40%), Postives = 755/770 (98.05%), Query Frame = 0

Query: 1   MADKKGTEQVVLRILDGEEGVDARNDLTNGSVASVPDFEPKETRSVRCAIPQSVVESSPS 60
           MADKKG EQVVLRILDGEEGVDARN LTNGSVAS PDFEPKETRS+RCAI QSVVESSPS
Sbjct: 1   MADKKGMEQVVLRILDGEEGVDARNGLTNGSVASFPDFEPKETRSLRCAITQSVVESSPS 60

Query: 61  HEISRMSSLKPPKIPV-SAVRRPSFARSSFSKPKSRLIEPPCPDGASLTEENAQAKSASG 120
           HEISRMSS KPPKIPV SAVRRPSFARSSFSKPKSRLIEPP PDGASL EENAQAKSASG
Sbjct: 61  HEISRMSSSKPPKIPVESAVRRPSFARSSFSKPKSRLIEPPRPDGASLAEENAQAKSASG 120

Query: 121 SPFSSSPKRDSPANIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180
           SPFSSSPK DSPA IATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE
Sbjct: 121 SPFSSSPKMDSPAKIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLELWKWCVLVLVIFCGRLFS 240
           KSRKKLKRAV+IEWIAFLCITACLISSLTIDKLLTKEIWGL LWKWCVLVLVIFCGRLFS
Sbjct: 181 KSRKKLKRAVVIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240

Query: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVLIWLALVLLAWGLLFDQSIKRSKKVN 300
           QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIV IWLALVLLAWGLLFDQSIKRSKKVN
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360
           EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP
Sbjct: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360

Query: 361 LMEMAERVGRVASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLIN 420
           LMEMAERVG  ASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLIN
Sbjct: 361 LMEMAERVGGAASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLIN 420

Query: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480
           VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF
Sbjct: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480

Query: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540
           RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL
Sbjct: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540

Query: 541 ASAFVLIVIVIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600
           ASAFVLIVI+IVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD
Sbjct: 541 ASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660
           VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS
Sbjct: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660

Query: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720
           VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY
Sbjct: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720

Query: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSAVAPMVSTTSRR 769
           GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNY+S+VAPMVSTT RR
Sbjct: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYLSSVAPMVSTTLRR 770

BLAST of Cp4.1LG18g03290 vs. ExPASy TrEMBL
Match: A0A6J1JDR1 (Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111484882 PE=3 SV=1)

HSP 1 Score: 1413 bits (3657), Expect = 0.0
Identity = 752/769 (97.79%), Postives = 756/769 (98.31%), Query Frame = 0

Query: 1   MADKKGTEQVVLRILDGEEGVDARNDLTNGSVASVPDFEPKETRSVRCAIPQSVVESSPS 60
           MADKKGTEQVVLRILDGEEGVDARN LTNGSVAS PDFE KETRSVRCAIPQSVVESSPS
Sbjct: 1   MADKKGTEQVVLRILDGEEGVDARNGLTNGSVASFPDFESKETRSVRCAIPQSVVESSPS 60

Query: 61  HEISRMSSLKPPKIPV-SAVRRPSFARSSFSKPKSRLIEPPCPDGASLTEENAQAKSASG 120
           HEISRMSSLKPPKIPV SAVRRPSFARSSFSKPKSRLIEPPCPDGAS  EENAQAKS SG
Sbjct: 61  HEISRMSSLKPPKIPVESAVRRPSFARSSFSKPKSRLIEPPCPDGASSAEENAQAKSTSG 120

Query: 121 SPFSSSPKRDSPANIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180
           SPFSSSPK DSPA IATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE
Sbjct: 121 SPFSSSPKMDSPAKIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLELWKWCVLVLVIFCGRLFS 240
           KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGL LWKWCVLVLVIFCGRLFS
Sbjct: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240

Query: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVLIWLALVLLAWGLLFDQSIKRSKKVN 300
           QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIV IWLALVLLAWGLLFDQSIKRSKKVN
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360
           EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILR LSGPP
Sbjct: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPP 360

Query: 361 LMEMAERVGRVASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLIN 420
           LMEMAERVGRVAS+GQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQ KVSAWTMRGLIN
Sbjct: 361 LMEMAERVGRVASAGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQAKVSAWTMRGLIN 420

Query: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480
           VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF
Sbjct: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480

Query: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540
           RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL
Sbjct: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540

Query: 541 ASAFVLIVIVIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600
           ASAFVLIVI+IVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD
Sbjct: 541 ASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660
           VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS
Sbjct: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660

Query: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720
           VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY
Sbjct: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720

Query: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSAVAPMVSTTSR 768
           GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVS++APMVSTTSR
Sbjct: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSIAPMVSTTSR 769

BLAST of Cp4.1LG18g03290 vs. ExPASy TrEMBL
Match: A0A6J1H896 (Mechanosensitive ion channel protein OS=Cucurbita moschata OX=3662 GN=LOC111461449 PE=3 SV=1)

HSP 1 Score: 1405 bits (3638), Expect = 0.0
Identity = 750/770 (97.40%), Postives = 755/770 (98.05%), Query Frame = 0

Query: 1   MADKKGTEQVVLRILDGEEGVDARNDLTNGSVASVPDFEPKETRSVRCAIPQSVVESSPS 60
           MADKKG EQVVLRILDGEEGVDARN LTNGSVAS PDFEPKETRS+RCAI QSVVESSPS
Sbjct: 1   MADKKGMEQVVLRILDGEEGVDARNGLTNGSVASFPDFEPKETRSLRCAITQSVVESSPS 60

Query: 61  HEISRMSSLKPPKIPV-SAVRRPSFARSSFSKPKSRLIEPPCPDGASLTEENAQAKSASG 120
           HEISRMSS KPPKIPV SAVRRPSFARSSFSKPKSRLIEPP PDGASL EENAQAKSASG
Sbjct: 61  HEISRMSSSKPPKIPVESAVRRPSFARSSFSKPKSRLIEPPRPDGASLAEENAQAKSASG 120

Query: 121 SPFSSSPKRDSPANIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180
           SPFSSSPK DSPA IATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE
Sbjct: 121 SPFSSSPKMDSPAKIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLELWKWCVLVLVIFCGRLFS 240
           KSRKKLKRAV+IEWIAFLCITACLISSLTIDKLLTKEIWGL LWKWCVLVLVIFCGRLFS
Sbjct: 181 KSRKKLKRAVVIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240

Query: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVLIWLALVLLAWGLLFDQSIKRSKKVN 300
           QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIV IWLALVLLAWGLLFDQSIKRSKKVN
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360
           EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP
Sbjct: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360

Query: 361 LMEMAERVGRVASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLIN 420
           LMEMAERVG  ASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLIN
Sbjct: 361 LMEMAERVGGAASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLIN 420

Query: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480
           VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF
Sbjct: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480

Query: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540
           RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL
Sbjct: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540

Query: 541 ASAFVLIVIVIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600
           ASAFVLIVI+IVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD
Sbjct: 541 ASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660
           VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS
Sbjct: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660

Query: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720
           VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY
Sbjct: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720

Query: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSAVAPMVSTTSRR 769
           GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNY+S+VAPMVSTT RR
Sbjct: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYLSSVAPMVSTTLRR 770

BLAST of Cp4.1LG18g03290 vs. ExPASy TrEMBL
Match: A0A6J1D1P7 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111016536 PE=3 SV=1)

HSP 1 Score: 1256 bits (3250), Expect = 0.0
Identity = 660/769 (85.83%), Postives = 707/769 (91.94%), Query Frame = 0

Query: 1   MADKKGTEQVVLRILDGEEGVDARNDLTNGSVASVPDFEPKETRSVRCAIPQSVVESSPS 60
           MADK+GTEQVVLRILD E  VD   D T  SVAS PDFE KETRS+RC IPQS V SSPS
Sbjct: 1   MADKEGTEQVVLRILDSEGAVDGTKDHTKRSVASHPDFELKETRSIRCTIPQSGVGSSPS 60

Query: 61  HEISRMSSLKPPKIPV-SAVRRPSFARSSFSKPKSRLIEPPCPDGASLTEENAQAKSASG 120
            EI+RMS LKPPKIPV S VRRPSFARSSFSKPKSRL+EPPCP GA+L E+ AQAKS SG
Sbjct: 61  PEIARMSPLKPPKIPVESTVRRPSFARSSFSKPKSRLVEPPCPGGANLAEDKAQAKSTSG 120

Query: 121 SPFSSSPKRDSPANIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180
           SP++     +SPA I TATSP+++LKSAPITP+TPL+GS GSEEEDDEEVYKTAELKVKE
Sbjct: 121 SPYN-----NSPAKITTATSPRDALKSAPITPKTPLIGSIGSEEEDDEEVYKTAELKVKE 180

Query: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLELWKWCVLVLVIFCGRLFS 240
           +S +KLK  +LIEW+AFLCIT C I+SLTI KL T EIWGL LW+WCVLVLVIFCGRLFS
Sbjct: 181 RSGRKLKITILIEWVAFLCITGCFIASLTIHKLQTTEIWGLGLWQWCVLVLVIFCGRLFS 240

Query: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVLIWLALVLLAWGLLFDQSIKRSKKVN 300
           QWFINCLVFLIERNFLL+RKVLYFVYGLRKSVI+ IWLALVLLAWGLLFD S KRSKK N
Sbjct: 241 QWFINCLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDHSRKRSKKAN 300

Query: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360
           +ILNYVTRALAASL GAGLWL+KTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP
Sbjct: 301 DILNYVTRALAASLAGAGLWLLKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360

Query: 361 LMEMAERVGRVASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLIN 420
           L+EMAERVGR AS+GQLSFRHLKK+ DGGNEGKEEVIDVDKLKKMKQEK+SAWTMRGLIN
Sbjct: 361 LIEMAERVGRTASTGQLSFRHLKKQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLIN 420

Query: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480
           VIRSSGLSTISNTIENF EEE EQKDKEINSEWEA AAAYQIFRNVAKPGSKYIDEEDLF
Sbjct: 421 VIRSSGLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLF 480

Query: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540
           RFM+KEE++NVLPLFEG VETGKIKRKTLKNWLVNVY+ERKSLAHSLNDTKTAIEELN+L
Sbjct: 481 RFMSKEEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL 540

Query: 541 ASAFVLIVIVIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600
           ASA VLIVI+IVWLLLMG LTTQ+LVFISSQLLLVVFMFGNTA+T+FEAIIFVFVMHPFD
Sbjct: 541 ASAVVLIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660
           VGDRCVVDGVQM+VEEMNILTTIFLR+DNEKIFYPNSVLATKPISNFYRSPEMSDSI+FS
Sbjct: 601 VGDRCVVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRSPEMSDSIEFS 660

Query: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720
           VDFSTSIESIGALKARIKSYLESKPQFWRP HSV+VKEIENVNKMK+ LY+NHTINFQNY
Sbjct: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQNY 720

Query: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSAVAPMVSTTSR 768
           GD+SSRRSDLVLELKKI E+LGIKYHLLPQEV LN V + APMV  + R
Sbjct: 721 GDKSSRRSDLVLELKKILEELGIKYHLLPQEVYLNSVGSAAPMVPPSPR 764

BLAST of Cp4.1LG18g03290 vs. ExPASy TrEMBL
Match: A0A0A0LYZ9 (Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_1G595830 PE=3 SV=1)

HSP 1 Score: 1244 bits (3220), Expect = 0.0
Identity = 656/763 (85.98%), Postives = 705/763 (92.40%), Query Frame = 0

Query: 1   MADKKGTEQVVLRILDGEEGV-DARNDLTNGSVASVPDFEPKETRSVRCAIPQSVVESSP 60
           MADKKG EQ+VLRIL+GEEGV ++  DL   SV S PDF+ KETRS RC IPQSVV SSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVRESSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60

Query: 61  SHEISRMSSLKPPKIPVSAV-RRPSFARSSFSKPKSRLIEPPCPDGASLTEENAQAKSAS 120
           SHEISRM+  KPPKIP   V RR SFA SSF+KPKSRLIEPPCPDGASL EE   AKS  
Sbjct: 61  SHEISRMTPHKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAKST- 120

Query: 121 GSPFSSSPKRDSPANIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVK 180
                SS K DSPA I T TSPKE+LK+APITP+TPL+G+TG+EEEDDEEVY+TAELKVK
Sbjct: 121 ---LYSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKVK 180

Query: 181 EKSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLELWKWCVLVLVIFCGRLF 240
           EKS K+LK+ V++EWIAFLC+T CLI+SLTI+ L+TKEIWGL LWKWCVLVLVIFCGRLF
Sbjct: 181 EKSGKRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRLF 240

Query: 241 SQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVLIWLALVLLAWGLLFDQSIKRSKKV 300
           SQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVI+ IWLALVLLAWGLLFDQS KRSK+ 
Sbjct: 241 SQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKEG 300

Query: 301 NEILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGP 360
           N+ILNYVTRAL ASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILR LSGP
Sbjct: 301 NKILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGP 360

Query: 361 PLMEMAERVGRVASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLI 420
           PLMEMAERVGR AS+GQLSF+HLKKE D GNEGKEEVIDVDKLKKMKQEK+SAWTMRGLI
Sbjct: 361 PLMEMAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLI 420

Query: 421 NVIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDL 480
           NVIR SGLSTISNTIENFKEEE E+KDKEINSEWEA AAAYQIFRNVAKPGSKYIDEEDL
Sbjct: 421 NVIRGSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDL 480

Query: 481 FRFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNR 540
           FRFM+KEEIDNVLPLFEGG ETGKIKRKTLKNWLVNVY+ERKSLAHSLNDTKTAIEELN+
Sbjct: 481 FRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNK 540

Query: 541 LASAFVLIVIVIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPF 600
           L+SA +LIVI+I WLLLMGFLTTQVLVFISSQ+LLVVFMFGNTA+T+FEAIIFVFVMHPF
Sbjct: 541 LSSAVILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPF 600

Query: 601 DVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDF 660
           DVGDRCVVDGVQM+VEEMNILTTIFLRYDNEKIFYPNSVLATKPISN+YRSPEMSDSIDF
Sbjct: 601 DVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDF 660

Query: 661 SVDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQN 720
           SVDFSTSIESIGALKARIK+YLESKPQFWRPN+SV+VKEIENVNKMKL+L +NHTINFQN
Sbjct: 661 SVDFSTSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQN 720

Query: 721 YGDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSAVAP 761
           YGD+S+RRSDLVLELKKIFE+LGIKYHLLPQEVQLNYVS+ AP
Sbjct: 721 YGDKSNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAAP 759

BLAST of Cp4.1LG18g03290 vs. ExPASy TrEMBL
Match: A0A1S3BR08 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103492355 PE=3 SV=1)

HSP 1 Score: 1242 bits (3213), Expect = 0.0
Identity = 655/762 (85.96%), Postives = 700/762 (91.86%), Query Frame = 0

Query: 1   MADKKGTEQVVLRILDGEEGVDARN-DLTNGSVASVPDFEPKETRSVRCAIPQSVVESSP 60
           MADKKG EQ+VLRIL+GEEGV   + DL   SV S PDF+ KETRS RC IP+S+V SSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPRSLVGSSP 60

Query: 61  SHEISRMSSLKPPKIPVSAV-RRPSFARSSFSKPKSRLIEPPCPDGASLTEENAQAKSAS 120
           SHEISRM+ LKPPKIP   V RR SFA SSFSKPKSRLIEPPCPDG SL EE A AKS+ 
Sbjct: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIEPPCPDGESLAEEKALAKSS- 120

Query: 121 GSPFSSSPKRDSPANIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVK 180
                 SPK DSPA I   TSPKE+LK+ PITP+TPL+G+TG+EEEDDEEVYKTAELKVK
Sbjct: 121 ---LYGSPKVDSPAKITAVTSPKEALKATPITPKTPLIGTTGNEEEDDEEVYKTAELKVK 180

Query: 181 EKSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLELWKWCVLVLVIFCGRLF 240
           EKS K+LK+ V++EW+AFLC+T CLI+SLTID L+TKEIWGL LWKWCVLVLVIFCGRLF
Sbjct: 181 EKSEKRLKKTVIVEWVAFLCLTGCLIASLTIDTLVTKEIWGLGLWKWCVLVLVIFCGRLF 240

Query: 241 SQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVLIWLALVLLAWGLLFDQSIKRSKKV 300
           SQWFINCLVFLIERNFLLKRKVLYFVYGL+KSVI+ IWLALVLLAWGLLFDQS KRSKK 
Sbjct: 241 SQWFINCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKG 300

Query: 301 NEILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGP 360
           NEILNYVTRAL ASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILR LSGP
Sbjct: 301 NEILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGP 360

Query: 361 PLMEMAERVGRVASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLI 420
           PLMEMA RVGR AS+GQLSF+HLK+E D GNEGKEEVIDVDKLKKMKQEK+SAWTMRGLI
Sbjct: 361 PLMEMAGRVGRAASTGQLSFKHLKQESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLI 420

Query: 421 NVIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDL 480
           NVIR SGLSTISNTIENFKEEE EQKDKEINSEWEA AAAYQIFRNVAKPGSKYIDEEDL
Sbjct: 421 NVIRGSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDL 480

Query: 481 FRFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNR 540
           FRFM+KEEIDNVLPLFEGG ETGKIKRKTLKNWLVNVY+ERKSLAHSLNDTKTAIEELN+
Sbjct: 481 FRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNK 540

Query: 541 LASAFVLIVIVIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPF 600
           LASA VLIVI+I WLLLMGFLTTQVLVFISSQ+LLVVFMFGNTA+T+FEAIIFVFVMHPF
Sbjct: 541 LASAVVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPF 600

Query: 601 DVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDF 660
           DVGDRCVVDGVQM+VEEMNILTTIFLRYDNEKIFYPNSVLATKPISN+YRSPEMSDSIDF
Sbjct: 601 DVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDF 660

Query: 661 SVDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQN 720
           SVDFSTSIESIGALKARIK+YLESKPQFWRPN+SV+VKEIENVNKMKL+L +NHTINFQN
Sbjct: 661 SVDFSTSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQN 720

Query: 721 YGDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSAVA 760
           YGD+S+RRSDLVLELKKIFEDLGIKYHLLPQ VQLNY S+ A
Sbjct: 721 YGDKSNRRSDLVLELKKIFEDLGIKYHLLPQAVQLNYASSAA 758

BLAST of Cp4.1LG18g03290 vs. TAIR 10
Match: AT5G12080.1 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 697.2 bits (1798), Expect = 1.5e-200
Identity = 390/733 (53.21%), Postives = 520/733 (70.94%), Query Frame = 0

Query: 32  VASVPDFEPKETRSVRCAIPQS----VVESSPSHEISRM--SSLKPPKIP----VSAVRR 91
           V +VP  E    RS   A P+S        SPS EIS++  S  KPP+ P    V   +R
Sbjct: 17  VINVP-VEEASRRSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQR 76

Query: 92  PSFARSSFSKPKSRLIEPPCPDGASLTEENAQAKSASGSPFSSSPKRDSPANIATATSPK 151
            SFARS +SKPKSR ++P CP   S+ EE  + +  +G  FS    R SP N     S +
Sbjct: 77  KSFARSVYSKPKSRFVDPSCPVDTSILEEEVREQLGAGFSFS----RASPNN----KSNR 136

Query: 152 ESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKEKSRKKLKRAVLIEWIAFLCITA 211
                AP+TP   +V     E+++DEE+YK  ++K+  + R K+    LIE   F+ I +
Sbjct: 137 SVGSPAPVTPSKVVV-----EKDEDEEIYK--KVKLNREMRSKISTLALIESAFFVVILS 196

Query: 212 CLISSLTIDKLLTKEIWGLELWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVL 271
            L++SLTI+ L     WGLE+WKWCVLV+VIF G L + WF+  +VFLIE NFLL+RKVL
Sbjct: 197 ALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVL 256

Query: 272 YFVYGLRKSVIVLIWLALVLLAWGLLFDQSIKRSKKVNEILNYVTRALAASLIGAGLWLV 331
           YFV+GL+KSV V IWL L+L+AW LLF+  +KRS    ++L  +TR L + L GA  WLV
Sbjct: 257 YFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLV 316

Query: 332 KTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLMEMAERVGRVASSGQLSFRHL 391
           KTLL+KILAA+F    FFDRIQ+S+FHQY+L+TLSG PLME AERVGR  S+G LSF  +
Sbjct: 317 KTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATV 376

Query: 392 KKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLINVIRSSGLSTISNTIENFKEEEG 451
            K+   G   +++VID+ K+ KMK+EKVSAWTMR L+  +R+SGLSTIS+T++     EG
Sbjct: 377 VKK---GTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEG 436

Query: 452 -EQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLFRFMNKEEIDNVLPLFEGGVET 511
            EQ D+EI SE EA AAAY +FRNVA+P   YI+EEDL RFM KEE+D V PLF+G  ET
Sbjct: 437 KEQADREITSEMEALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAET 496

Query: 512 GKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRLASAFVLIVIVIVWLLLMGFLT 571
           G+I RK    W+V VY  R++LAHSLNDTKTA+++LN+L +A +++V V++WLLL+   T
Sbjct: 497 GRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVAT 556

Query: 572 TQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFDVGDRCVVDGVQMIVEEMNILT 631
           T+VL+F S+QL+ + F+ G+T K +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LT
Sbjct: 557 TKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLT 616

Query: 632 TIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFSVDFSTSIESIGALKARIKSYL 691
           T+FL+ +NEK++YPN+VLATKPISN++RSP M ++++FS+ FST +  I  LK RI  YL
Sbjct: 617 TVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYL 676

Query: 692 ESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNYGDRSSRRSDLVLELKKIFEDL 751
           E  PQ W P HSV+VKEIEN+NK+K++LY +HTI FQ   +R+ RR++L L +K++ EDL
Sbjct: 677 EQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDL 730

Query: 752 GIKYHLLPQEVQL 754
            I Y LLPQ++ L
Sbjct: 737 HIDYTLLPQDINL 730

BLAST of Cp4.1LG18g03290 vs. TAIR 10
Match: AT5G12080.2 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 697.2 bits (1798), Expect = 1.5e-200
Identity = 390/733 (53.21%), Postives = 520/733 (70.94%), Query Frame = 0

Query: 32  VASVPDFEPKETRSVRCAIPQS----VVESSPSHEISRM--SSLKPPKIP----VSAVRR 91
           V +VP  E    RS   A P+S        SPS EIS++  S  KPP+ P    V   +R
Sbjct: 17  VINVP-VEEASRRSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQR 76

Query: 92  PSFARSSFSKPKSRLIEPPCPDGASLTEENAQAKSASGSPFSSSPKRDSPANIATATSPK 151
            SFARS +SKPKSR ++P CP   S+ EE  + +  +G  FS    R SP N     S +
Sbjct: 77  KSFARSVYSKPKSRFVDPSCPVDTSILEEEVREQLGAGFSFS----RASPNN----KSNR 136

Query: 152 ESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKEKSRKKLKRAVLIEWIAFLCITA 211
                AP+TP   +V     E+++DEE+YK  ++K+  + R K+    LIE   F+ I +
Sbjct: 137 SVGSPAPVTPSKVVV-----EKDEDEEIYK--KVKLNREMRSKISTLALIESAFFVVILS 196

Query: 212 CLISSLTIDKLLTKEIWGLELWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVL 271
            L++SLTI+ L     WGLE+WKWCVLV+VIF G L + WF+  +VFLIE NFLL+RKVL
Sbjct: 197 ALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVL 256

Query: 272 YFVYGLRKSVIVLIWLALVLLAWGLLFDQSIKRSKKVNEILNYVTRALAASLIGAGLWLV 331
           YFV+GL+KSV V IWL L+L+AW LLF+  +KRS    ++L  +TR L + L GA  WLV
Sbjct: 257 YFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLV 316

Query: 332 KTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLMEMAERVGRVASSGQLSFRHL 391
           KTLL+KILAA+F    FFDRIQ+S+FHQY+L+TLSG PLME AERVGR  S+G LSF  +
Sbjct: 317 KTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATV 376

Query: 392 KKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLINVIRSSGLSTISNTIENFKEEEG 451
            K+   G   +++VID+ K+ KMK+EKVSAWTMR L+  +R+SGLSTIS+T++     EG
Sbjct: 377 VKK---GTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEG 436

Query: 452 -EQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLFRFMNKEEIDNVLPLFEGGVET 511
            EQ D+EI SE EA AAAY +FRNVA+P   YI+EEDL RFM KEE+D V PLF+G  ET
Sbjct: 437 KEQADREITSEMEALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAET 496

Query: 512 GKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRLASAFVLIVIVIVWLLLMGFLT 571
           G+I RK    W+V VY  R++LAHSLNDTKTA+++LN+L +A +++V V++WLLL+   T
Sbjct: 497 GRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVAT 556

Query: 572 TQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFDVGDRCVVDGVQMIVEEMNILT 631
           T+VL+F S+QL+ + F+ G+T K +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LT
Sbjct: 557 TKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLT 616

Query: 632 TIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFSVDFSTSIESIGALKARIKSYL 691
           T+FL+ +NEK++YPN+VLATKPISN++RSP M ++++FS+ FST +  I  LK RI  YL
Sbjct: 617 TVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYL 676

Query: 692 ESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNYGDRSSRRSDLVLELKKIFEDL 751
           E  PQ W P HSV+VKEIEN+NK+K++LY +HTI FQ   +R+ RR++L L +K++ EDL
Sbjct: 677 EQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDL 730

Query: 752 GIKYHLLPQEVQL 754
            I Y LLPQ++ L
Sbjct: 737 HIDYTLLPQDINL 730

BLAST of Cp4.1LG18g03290 vs. TAIR 10
Match: AT5G12080.3 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 697.2 bits (1798), Expect = 1.5e-200
Identity = 390/733 (53.21%), Postives = 520/733 (70.94%), Query Frame = 0

Query: 32  VASVPDFEPKETRSVRCAIPQS----VVESSPSHEISRM--SSLKPPKIP----VSAVRR 91
           V +VP  E    RS   A P+S        SPS EIS++  S  KPP+ P    V   +R
Sbjct: 17  VINVP-VEEASRRSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQR 76

Query: 92  PSFARSSFSKPKSRLIEPPCPDGASLTEENAQAKSASGSPFSSSPKRDSPANIATATSPK 151
            SFARS +SKPKSR ++P CP   S+ EE  + +  +G  FS    R SP N     S +
Sbjct: 77  KSFARSVYSKPKSRFVDPSCPVDTSILEEEVREQLGAGFSFS----RASPNN----KSNR 136

Query: 152 ESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKEKSRKKLKRAVLIEWIAFLCITA 211
                AP+TP   +V     E+++DEE+YK  ++K+  + R K+    LIE   F+ I +
Sbjct: 137 SVGSPAPVTPSKVVV-----EKDEDEEIYK--KVKLNREMRSKISTLALIESAFFVVILS 196

Query: 212 CLISSLTIDKLLTKEIWGLELWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVL 271
            L++SLTI+ L     WGLE+WKWCVLV+VIF G L + WF+  +VFLIE NFLL+RKVL
Sbjct: 197 ALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVL 256

Query: 272 YFVYGLRKSVIVLIWLALVLLAWGLLFDQSIKRSKKVNEILNYVTRALAASLIGAGLWLV 331
           YFV+GL+KSV V IWL L+L+AW LLF+  +KRS    ++L  +TR L + L GA  WLV
Sbjct: 257 YFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLV 316

Query: 332 KTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLMEMAERVGRVASSGQLSFRHL 391
           KTLL+KILAA+F    FFDRIQ+S+FHQY+L+TLSG PLME AERVGR  S+G LSF  +
Sbjct: 317 KTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATV 376

Query: 392 KKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLINVIRSSGLSTISNTIENFKEEEG 451
            K+   G   +++VID+ K+ KMK+EKVSAWTMR L+  +R+SGLSTIS+T++     EG
Sbjct: 377 VKK---GTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEG 436

Query: 452 -EQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLFRFMNKEEIDNVLPLFEGGVET 511
            EQ D+EI SE EA AAAY +FRNVA+P   YI+EEDL RFM KEE+D V PLF+G  ET
Sbjct: 437 KEQADREITSEMEALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAET 496

Query: 512 GKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRLASAFVLIVIVIVWLLLMGFLT 571
           G+I RK    W+V VY  R++LAHSLNDTKTA+++LN+L +A +++V V++WLLL+   T
Sbjct: 497 GRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVAT 556

Query: 572 TQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFDVGDRCVVDGVQMIVEEMNILT 631
           T+VL+F S+QL+ + F+ G+T K +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LT
Sbjct: 557 TKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLT 616

Query: 632 TIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFSVDFSTSIESIGALKARIKSYL 691
           T+FL+ +NEK++YPN+VLATKPISN++RSP M ++++FS+ FST +  I  LK RI  YL
Sbjct: 617 TVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYL 676

Query: 692 ESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNYGDRSSRRSDLVLELKKIFEDL 751
           E  PQ W P HSV+VKEIEN+NK+K++LY +HTI FQ   +R+ RR++L L +K++ EDL
Sbjct: 677 EQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDL 730

Query: 752 GIKYHLLPQEVQL 754
            I Y LLPQ++ L
Sbjct: 737 HIDYTLLPQDINL 730

BLAST of Cp4.1LG18g03290 vs. TAIR 10
Match: AT5G19520.1 (mechanosensitive channel of small conductance-like 9 )

HSP 1 Score: 623.2 bits (1606), Expect = 2.7e-178
Identity = 360/759 (47.43%), Postives = 499/759 (65.74%), Query Frame = 0

Query: 8   EQVVLRILDGEEGVDARNDLTNGSVASVPDFEPKETRSVRCAIPQSVVESSPSHEISRMS 67
           E+VV+ + D E+  D R   +   +AS     P     +  + P   + S P+ EI + S
Sbjct: 10  EEVVINVSDKEDSKDPRASPSFNPLAS-----PDSDAGIEKSKPVPPI-SIPTPEIYKFS 69

Query: 68  SL--KPPKI--PVSAVRRPSFARSSFSKPKSRLIEPPC---------PDGASLTEENAQA 127
               KPPKI  P   VRR S +RS +SKPKSR  E              G SL E+    
Sbjct: 70  GSVHKPPKIPSPEGLVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLREQFGAG 129

Query: 128 KSASGSPFSSSPKRDSPANIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAE 187
             A GS   +SP   S  ++A+A   K +                  E +++EE+YK  +
Sbjct: 130 SFARGSFDRASPNNKSNRSVASAALSKVA----------------EEEPDENEEIYK--K 189

Query: 188 LKVKEKSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLELWKWCVLVLVIFC 247
           +K+    R  +K    +E + F+ I   LI SLTID +    IWGLE WKWCVLV+V   
Sbjct: 190 VKLHRVKRSGMKPLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLS 249

Query: 248 GRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVLIWLALVLLAWGLLFDQSIKR 307
           G L + WF++ +VF+IE+N+LL++KVLYFV+GL+K+V V IW +LVL+AW  LFD  +KR
Sbjct: 250 GMLVTNWFMHFVVFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKR 309

Query: 308 SKKVNEILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRT 367
           ++K    L+++T  + + L+G+ L+LVKT  +K+LA+ F    FF+RIQES+FHQY+L+T
Sbjct: 310 TRKTKRFLDFITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQT 369

Query: 368 LSGPPLMEMAERVGRVASSGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTM 427
           LSGPPL+E AE VGRV S+G LSF   K     G    ++VID+ K+ +MKQEKVSAWTM
Sbjct: 370 LSGPPLIEEAENVGRVPSTGHLSFTRTK----DGKVKDKKVIDMGKVHRMKQEKVSAWTM 429

Query: 428 RGLINVIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYID 487
           R LI  + +SG+STIS+T++    ++ E+ DKEI +E EA AAAY +F NVAKP   YI+
Sbjct: 430 RVLIEAVGTSGISTISSTLDEVNNKK-ERTDKEITNEMEAVAAAYDVFNNVAKPNHNYIE 489

Query: 488 EEDLFRFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIE 547
           E+DL RFM KEE+D VLPL E   +TGKI RKT   W+VNVY  RK++ HSLNDTKTA++
Sbjct: 490 EDDLLRFMIKEEVDLVLPLIE-DADTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVK 549

Query: 548 ELNRLASAFVLIVIVIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFV 607
           +L++L +  + ++  IVW++L+   +T++L+  SSQ L + FM G+T K IFE+ +FVFV
Sbjct: 550 QLDKLITGILTVITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFV 609

Query: 608 MHPFDVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSD 667
           MHP+DVGDRCVVDGV ++VEE+++LTT+FL+ DNEK+FYPNSVL +KPISNFYRSP+M D
Sbjct: 610 MHPYDVGDRCVVDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGD 669

Query: 668 SIDFSVDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTI 727
            +DF + FST  E IG LK +I  YL +  Q W P   V+V+ IEN+NK+ L++ + HTI
Sbjct: 670 YVDFGIAFSTPAEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTI 729

Query: 728 NFQNYGDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQL 754
           NFQ Y ++S RR+ L++ +K+I EDL I Y LLPQ+V L
Sbjct: 730 NFQVYVEKSLRRTALIIAIKRILEDLEIDYTLLPQDVNL 738

BLAST of Cp4.1LG18g03290 vs. TAIR 10
Match: AT3G14810.1 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 530.8 bits (1366), Expect = 1.8e-150
Identity = 313/718 (43.59%), Postives = 452/718 (62.95%), Query Frame = 0

Query: 80  RRPSFARSSFSKPKSRLIEPPCPDGASLTEENAQAKSASG---SPF-SSSPKRDSPANIA 139
           ++P  +R   +K KSRL +PP P   ++ +   ++   SG   S F   SPK  +P    
Sbjct: 185 KKPMLSR---NKTKSRLQDPPTPTHPAIDKTEMKSGRRSGIFKSGFLGKSPKAGTP---- 244

Query: 140 TATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKEKSRKKLKRAVLIEWIA 199
                                G  G EEE++E+ +   +L  +E  R KL   V +EWI+
Sbjct: 245 ---------------------GRNGFEEEEEEDPFLDEDLP-EEFKRDKLSFWVFLEWIS 304

Query: 200 FLCITACLISSLTIDKLLTKEIWGLELWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFL 259
            + I   L+ SLTI  L  K  W L+LWKW V VLV+ CGRL S W +  +VFL+E+NF 
Sbjct: 305 LVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKNFT 364

Query: 260 LKRKVLYFVYGLRKSVIVLIWLALVLLAWGLLFDQSIKRSKKVNEILNYVTRALAASLIG 319
            +++VLYFVYG+RKSV   +WL LVLLAW  LFD+ ++R  + +  L YVTR L   L+ 
Sbjct: 365 WRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETR-STALRYVTRVLVCLLVA 424

Query: 320 AGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLME---MAERVGRVAS 379
             +WLVKT+LVK+LA+SF  + +FDRIQES+F QY++ TLSGPPLME   M E   +VA 
Sbjct: 425 LIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAE 484

Query: 380 SGQ------------------LSFRHLKK----ERDGGNEGKE-EVIDVDKLKKMKQEKV 439
             +                   SF  + K     R G   G++ E I +D+LK+M  + V
Sbjct: 485 DVKSLEKLAGAKLPPALKATVKSFMKVGKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNV 544

Query: 440 SAWTMRGLINVIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPG 499
           SAW M+ L+N+I    +ST+   +++  +E  ++    I SE+EA  AA +IF NV +PG
Sbjct: 545 SAWNMKRLMNIILKGAISTLDQNMQDTTQE--DEDATHIRSEYEAKCAARKIFHNVTEPG 604

Query: 500 SKYIDEEDLFRFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDT 559
           S+YI  ED  RF+ +EE +  + LFEG  E+ KI +  LKNW+V  + ER++LA +LNDT
Sbjct: 605 SRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVKAFRERRALALTLNDT 664

Query: 560 KTAIEELNRLASAFVLIVIVIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAI 619
           KTA++ L+R+ +  + I+I+I+WLL++G  TT+ L+ +SSQLLLV F+FGN+ KTIFEAI
Sbjct: 665 KTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVFGNSCKTIFEAI 724

Query: 620 IFVFVMHPFDVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRS 679
           IF+FVMHPFDVGDRC +DGVQ++VEEMNILTT+FLRYDN+KI YPNSVL TKPI+N+YRS
Sbjct: 725 IFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRS 784

Query: 680 PEMSDSIDFSVDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLY 739
           P+M D+++F V  +T  E I A+K RI SY+++K  +W P   ++   ++++N +K++++
Sbjct: 785 PDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVW 844

Query: 740 INHTINFQNYGDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSAVAPMVSTTS 768
           + H +N Q+ G+R  RR  L+ E+ K   +L I+Y L P  +    V ++ P  + TS
Sbjct: 845 LTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNIN---VRSLPPTANPTS 867