Cp4.1LG18g02950 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG18g02950
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionPectinesterase
LocationCp4.1LG18: 4447301 .. 4451720 (-)
RNA-Seq ExpressionCp4.1LG18g02950
SyntenyCp4.1LG18g02950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTCCCACCTCGAATTTATTCAAGGCCAAAAACCACCCCCACCAAGGAGAGTTTCGGGAGAAATGTCTCGGCCAGCCGTTGCTTCATCTTTTCCGGCGACGGTGGCGCTCGTACTGGCGGTGGTCCAACTCATCACCATACTTATTCCTGCAGCCATTGTCGTCGAAGCTCGCACGAAGACGATTCCGGCAGAGCCATCGCAGCTCGACGAATGGATCGCACACAACATGAGAGAGTACCGTCAACGGAAGGCGAACGAGACAGGAGCCAAGACGCTGGATCCGCGGCTTGCTAAGGCGGAGGATTGCGCTCGAATCATCACCGTTAAACAAGACGGACAAGGAAATTTCTCAACGATCACTCAAGCGATCGAGAGCATTCCGTCCGGAAATCGTGAGCGAGTGGTGGTGTGGATCGGCGGTGGAACTTACCGCGAGAAGATCACGATCGATTCTTCGAAGCCGTTCGTGACGCTGTACGGTCAGACAGGAAAAATGCCGGTCATATCGTACGACGGAACGGCGTCGGAATTTGGTACAGCCGAGAGCGCGACGGTGGCGGTGGAATCGGATTATTTCGTCGCCGTCAATGTTGCGTTTGTGGTGAGTTTCTCTTCTTCTTCTCCTCATTTTTGTGGGGTAAGTTTTGTAAATAGTGAAAGTTATGGGGCGCAAAAAAATTTTAGTAACTTATTATTGCGTGACTACTAATTATTTATTTATTTATTTATATTTTTTTCCGGAAAAAATTCATGCATTTATTCGCCATCAAAATTAAGCATGAGTTTTCACGTTGAAATCTTGACATTTTATTGCACAATTGGTTATTTCTGTTAGAAAAGTTCCCTTTTGAGATGACTATACCTTGTTGGATAAAAAATTTGTAGGTTTTTAATCACTCTTGTTGATTGCAATGAGATTCCTGCTCGGTAATATTGTTCGGTGTAGACTCGAAAATCTGTAGTTTCATTTTAGATATCAATCTGTCATGATTTGGTTGTCAGGATCAATAGGACATGCATGAATTGGTAAGAAATGTAATACCTGTGCAATTACAACCACATCGGTTGGAGAGGAGAACGAAACATTGTAAGAGTGTGGAAACCTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATTCCTGGTGTTGAATCTTCTTCCCAGTAGATGTGTCATATCTGTCGGAGAGGAGAACAGGAGAATTCCACACAAAACTTTGAGGGAACCCCGAATGGGAAAGCTCAAAGAGGACAATATCCGTTAGCGGTGGGCCGATTATAAATGGTATCGGAACTAGACACCAGGTTGTGTGCTAGCAAGGATGCTAAGCCCCCAAGGGAGTGGACTGTGAGATTCTATATTGGTTCGATTCCGTTAGTTTGGTTTCAAACTAACCTTTCTAGTTCAAAATTGTACGATCAAACTAACCTTTCTAGTTCAAAATTGTACGATCAAACTAACCTTTTTAGTTCAAAATTGTACGATCTCTCCTAACGTGTTTTGAATGAATGAAAATGAAATTGTAGTGATTAACAAAAAATGAAGATTGGGTATGATGAATTTGTTGATGCAGAACTCAGCTCCAATGCCGGATGCAGGAAGAACAGGAGGACAAGCAGTGGCAATGAGGATATCAGGGGATAAGGCAGCATTCCACCACTGCCAATTCATAGGGTTTCAGGACACTTTATGCGATGACAGAGGCAGACATTTTTTCAAGGATTGTTACATCGAAGGCACTGTGGACTTCATCTTTGGAAATGGCAAATCCCTCTACTTGGTGAGTGGGTGTCTATTTTTTTACTATGATCGATCTGGGATCTGGTAAGTGCGTATCTATTTTTTTACTATGATCGATCTGGTAAGTGCGTATCTATTTTTTTACTATGATCGATCTGGTAAGTGGGTGTCTATTTTTTTACTATGATCGATCTGGTAAGTGGGTGTCTATTTTTTTACTATGATCGATCTGGTAAGTGGGTGTCTATTTTTTTTACTATGATCGATCTGGTAAGTGGGTGCCAAGTAGGTATCTGTAAGTAGGTATCTATTTTTACCAGAGCTAGTAAGTAGGTATCTACTCACAAAGAAATTATTCATTACCTAATTCTTCATCTAAAATTTCTATCAACGTATAAGCCATCATCGAGCTGGTGAGTAGGTTCGACAATTGTTGTGTTAGAGGTACTATGCAAAAAAATTCCAAATTTATATCCATTTGTTGTCATCAACCATTTCATCCATATATGTGTGTGTATTTGTGACAGAAAACAGTAATAAACTCAGTGGCGGAAGGGACAGGAGTGATCACAGCTCAAGCAAGGGAGAATTCCACAGACGACAGCGGCTTCACATTTGCATATTGCAACATCACTGGAACTGGAAACACCTATTTGGGAAGAGCATGGAAGGAGAGAACTCGTGTGGTTTTTGCTTACACTTACATGGGAACACTCATTAACACCCAAGGATGGTCCGATAAAATGCAGGGAACCGAACCTCGAAAGTGAGCAAAATCACGCACATAATAATATCATCATCATCATCATCGTCATCATCATCTCATTGCTAGTTGATTGATTCATTTTGATTATCGACCGTGTTGACAGGAGTATGTACTACGGTGAGTACAAATGCAAAGGACCCGGGGCAGACAGCACGGGACGAGCACACTACACGAGAATACTCACGGACTACGAAGCGAAGGCGTTTTTGAGTATGACTTATATCCATGGAAACAAATGGGTTCTACCACCTCCAAGGCTGTCCCTTCTCCCGGTGTCATCTCAATGCCATGGCTGATTATGTTGTCTCGTGTTTCTTTTTGGATAAAGTTTTGGTTGATTCTTGTTGTTGTTGTACCAAACCGATCAATTTCTTGAGCTTTCCAGGAAGCCTCTCCTCTCTCTCAGTTGATCTCTTCTTTTAATCATTTATATGTTCTTACATTTACAAACAATGCTGCGCGCAGTTTTGCAAGTTTAGGCAAAAATATGATCATGAATGGGCCTTTGCCCACGCATTTGT

mRNA sequence

CCTCCCACCTCGAATTTATTCAAGGCCAAAAACCACCCCCACCAAGGAGAGTTTCGGGAGAAATGTCTCGGCCAGCCGTTGCTTCATCTTTTCCGGCGACGGTGGCGCTCGTACTGGCGGTGGTCCAACTCATCACCATACTTATTCCTGCAGCCATTGTCGTCGAAGCTCGCACGAAGACGATTCCGGCAGAGCCATCGCAGCTCGACGAATGGATCGCACACAACATGAGAGAGTACCGTCAACGGAAGGCGAACGAGACAGGAGCCAAGACGCTGGATCCGCGGCTTGCTAAGGCGGAGGATTGCGCTCGAATCATCACCGTTAAACAAGACGGACAAGGAAATTTCTCAACGATCACTCAAGCGATCGAGAGCATTCCGTCCGGAAATCGTGAGCGAGTGGTGGTGTGGATCGGCGGTGGAACTTACCGCGAGAAGATCACGATCGATTCTTCGAAGCCGTTCGTGACGCTGTACGGTCAGACAGGAAAAATGCCGGTCATATCGTACGACGGAACGGCGTCGGAATTTGGTACAGCCGAGAGCGCGACGGTGGCGGTGGAATCGGATTATTTCGTCGCCGTCAATGTTGCGTTTGTGAACTCAGCTCCAATGCCGGATGCAGGAAGAACAGGAGGACAAGCAGTGGCAATGAGGATATCAGGGGATAAGGCAGCATTCCACCACTGCCAATTCATAGGGTTTCAGGACACTTTATGCGATGACAGAGGCAGACATTTTTTCAAGGATTGTTACATCGAAGGCACTGTGGACTTCATCTTTGGAAATGGCAAATCCCTCTACTTGAAAACAGTAATAAACTCAGTGGCGGAAGGGACAGGAGTGATCACAGCTCAAGCAAGGGAGAATTCCACAGACGACAGCGGCTTCACATTTGCATATTGCAACATCACTGGAACTGGAAACACCTATTTGGGAAGAGCATGGAAGGAGAGAACTCGTGTGGTTTTTGCTTACACTTACATGGGAACACTCATTAACACCCAAGGATGGTCCGATAAAATGCAGGGAACCGAACCTCGAAAGAGTATGTACTACGGTGAGTACAAATGCAAAGGACCCGGGGCAGACAGCACGGGACGAGCACACTACACGAGAATACTCACGGACTACGAAGCGAAGGCGTTTTTGAGTATGACTTATATCCATGGAAACAAATGGGTTCTACCACCTCCAAGGCTGTCCCTTCTCCCGGTGTCATCTCAATGCCATGGCTGATTATGTTGTCTCGTGTTTCTTTTTGGATAAAGTTTTGGTTGATTCTTGTTGTTGTTGTACCAAACCGATCAATTTCTTGAGCTTTCCAGGAAGCCTCTCCTCTCTCTCAGTTGATCTCTTCTTTTAATCATTTATATGTTCTTACATTTACAAACAATGCTGCGCGCAGTTTTGCAAGTTTAGGCAAAAATATGATCATGAATGGGCCTTTGCCCACGCATTTGT

Coding sequence (CDS)

ATGTCTCGGCCAGCCGTTGCTTCATCTTTTCCGGCGACGGTGGCGCTCGTACTGGCGGTGGTCCAACTCATCACCATACTTATTCCTGCAGCCATTGTCGTCGAAGCTCGCACGAAGACGATTCCGGCAGAGCCATCGCAGCTCGACGAATGGATCGCACACAACATGAGAGAGTACCGTCAACGGAAGGCGAACGAGACAGGAGCCAAGACGCTGGATCCGCGGCTTGCTAAGGCGGAGGATTGCGCTCGAATCATCACCGTTAAACAAGACGGACAAGGAAATTTCTCAACGATCACTCAAGCGATCGAGAGCATTCCGTCCGGAAATCGTGAGCGAGTGGTGGTGTGGATCGGCGGTGGAACTTACCGCGAGAAGATCACGATCGATTCTTCGAAGCCGTTCGTGACGCTGTACGGTCAGACAGGAAAAATGCCGGTCATATCGTACGACGGAACGGCGTCGGAATTTGGTACAGCCGAGAGCGCGACGGTGGCGGTGGAATCGGATTATTTCGTCGCCGTCAATGTTGCGTTTGTGAACTCAGCTCCAATGCCGGATGCAGGAAGAACAGGAGGACAAGCAGTGGCAATGAGGATATCAGGGGATAAGGCAGCATTCCACCACTGCCAATTCATAGGGTTTCAGGACACTTTATGCGATGACAGAGGCAGACATTTTTTCAAGGATTGTTACATCGAAGGCACTGTGGACTTCATCTTTGGAAATGGCAAATCCCTCTACTTGAAAACAGTAATAAACTCAGTGGCGGAAGGGACAGGAGTGATCACAGCTCAAGCAAGGGAGAATTCCACAGACGACAGCGGCTTCACATTTGCATATTGCAACATCACTGGAACTGGAAACACCTATTTGGGAAGAGCATGGAAGGAGAGAACTCGTGTGGTTTTTGCTTACACTTACATGGGAACACTCATTAACACCCAAGGATGGTCCGATAAAATGCAGGGAACCGAACCTCGAAAGAGTATGTACTACGGTGAGTACAAATGCAAAGGACCCGGGGCAGACAGCACGGGACGAGCACACTACACGAGAATACTCACGGACTACGAAGCGAAGGCGTTTTTGAGTATGACTTATATCCATGGAAACAAATGGGTTCTACCACCTCCAAGGCTGTCCCTTCTCCCGGTGTCATCTCAATGCCATGGCTGA

Protein sequence

MSRPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYRQRKANETGAKTLDPRLAKAEDCARIITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGGGTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFVNSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERTRVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDYEAKAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG
Homology
BLAST of Cp4.1LG18g02950 vs. ExPASy Swiss-Prot
Match: Q9LY17 (Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 1.3e-96
Identity = 172/357 (48.18%), Postives = 246/357 (68.91%), Query Frame = 0

Query: 26  ILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYRQRKANETGAKTLDPRLAKAEDCARI 85
           ++  + +V+   T  IP   +Q+ +W   N++ Y QRK       TLDP L  AE   +I
Sbjct: 13  VVFASPVVLATDTDPIPENRAQIPQWFKTNVKPYSQRKG------TLDPALEAAEAARQI 72

Query: 86  ITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGGGTYREKITIDSSKPFVTLYGQTGKM 145
           ITV Q G  NF T+ +AI+SIP+GN+ RV++ +  G Y EK+TID ++PF+TL GQ G  
Sbjct: 73  ITVNQKGGANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTIDIARPFITLLGQPGAE 132

Query: 146 PVISYDGTASEFGTAESATVAVESDYFVAVNVAFVNSAPMPDAGRTGGQAVAMRISGDKA 205
            V++Y GTA+++GT ESAT+ V ++YF A ++   N+APMP  G + GQA+AMRI+ DKA
Sbjct: 133 TVLTYHGTAAQYGTVESATLIVWAEYFQAAHLTIKNTAPMPKPG-SQGQALAMRINADKA 192

Query: 206 AFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTVINSVAEGTGVITA 265
           AF+ C+F GFQDTLCDD+G HFFKDCYIEGT DFIFG G SLYL T +++V +G  VITA
Sbjct: 193 AFYSCRFHGFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGASLYLNTQLHAVGDGLRVITA 252

Query: 266 QARENSTDDSGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINTQGWSDKM-Q 325
           Q R+++T+ +G+TF +C +TGTG   YLGR+W    +VV+A+T M +++N  GW + + +
Sbjct: 253 QGRQSATEQNGYTFVHCKVTGTGTGIYLGRSWMSHPKVVYAFTEMTSVVNPSGWRENLNR 312

Query: 326 GTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDYEAKAFLSMTYIHGNKWVLPPPR 381
           G +  K+++YGEYKC GPG+    R  YT+ +   E   FL++ YI G+ W+LPPP+
Sbjct: 313 GYD--KTVFYGEYKCFGPGSHLEKRVPYTQDIDKNEVTPFLTLGYIKGSTWLLPPPK 360

BLAST of Cp4.1LG18g02950 vs. ExPASy Swiss-Prot
Match: Q84WM7 (Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 3.2e-95
Identity = 169/342 (49.42%), Postives = 232/342 (67.84%), Query Frame = 0

Query: 41  IPAEPSQLDEWIAHNMREYRQRKANETGAKTLDPRLAKAEDCARIITVKQDGQGNFSTIT 100
           IP    Q+ +W   N+    QRK        LDP L  AE   RII V   G G F T+T
Sbjct: 29  IPEGKPQVAQWFNANVGPLAQRKG-------LDPALVAAEAAPRIINVNPKG-GEFKTLT 88

Query: 101 QAIESIPSGNRERVVVWIGGGTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTA 160
            AI+S+P+GN +RV++ +  G Y+EK+TID +KPF+TL GQ   MPVI+YDGTA+++GT 
Sbjct: 89  DAIKSVPAGNTKRVIIKMAPGEYKEKVTIDRNKPFITLMGQPNAMPVITYDGTAAKYGTV 148

Query: 161 ESATVAVESDYFVAVNVAFVNSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLC 220
           +SA++ + SDYF+AVN+   N+AP PD    G QA++MRISG+ AAF++C+F GFQDT+C
Sbjct: 149 DSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGNFAAFYNCKFYGFQDTIC 208

Query: 221 DDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFA 280
           DD G HFFKDCY+EGT DFIFG+G S+YL T ++ V +G  VI A A +++ + SG++F 
Sbjct: 209 DDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVGDGIRVIAAHAGKSAEEKSGYSFV 268

Query: 281 YCNITGT-GNTYLGRAWKERTRVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCK 340
           +C +TGT G  YLGRAW    +VV+AYT M +++N  GW +        K+++YGEYKC 
Sbjct: 269 HCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENKTPAHD-KTVFYGEYKCS 328

Query: 341 GPGADSTGRAHYTRILTDYEAKAFLSMTYIHGNKWVLPPPRL 382
           GPG+    R  +T+ + D EA  FLS+ YI G+KW+LPPP L
Sbjct: 329 GPGSHKAKRVPFTQDIDDKEANRFLSLGYIQGSKWLLPPPAL 361

BLAST of Cp4.1LG18g02950 vs. ExPASy Swiss-Prot
Match: Q9FKF3 (Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2)

HSP 1 Score: 349.4 bits (895), Expect = 5.4e-95
Identity = 169/364 (46.43%), Postives = 239/364 (65.66%), Query Frame = 0

Query: 17  VLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYRQRKANETGAKTLDPRL 76
           V  +V ++ ++I + +V       IP    ++++W   N                     
Sbjct: 6   VSLIVTILLVVITSPVVFGNDAAPIPENKGRIEQWFNTN--------------------- 65

Query: 77  AKAEDCARIITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGGGTYREKITIDSSKPFV 136
                      VKQ+G+G+F TIT+AI S+ +GN  RV++ IG G Y+EK+TID SKPF+
Sbjct: 66  -----------VKQNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFI 125

Query: 137 TLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFVNSAPMPDAGRTGGQAV 196
           TLYG    MPV+++DGTA+++GT +SAT+ V SDYF+AVN+   NSAPMPD  R G QA+
Sbjct: 126 TLYGHPNAMPVLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQAL 185

Query: 197 AMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTVINSV 256
           +MRISG+KAAF++C+F G+QDT+CDD G HFFKDCYIEGT DFIFG+G+SLYL T +N V
Sbjct: 186 SMRISGNKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVV 245

Query: 257 AEGTGVITAQARENSTDDSGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINT 316
            +G  VITA A +++ + SG++F +C +TGTG   YLGR+W    +VV+AYT M +++N 
Sbjct: 246 GDGIRVITAHAGKSAAEKSGYSFVHCKVTGTGTGIYLGRSWMSHPKVVYAYTDMSSVVNP 305

Query: 317 QGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDYEAKAFLSMTYIHGNKWV 376
            GW +  +     K+++YGEYKC G G+    R  YT+ + D EAK F+S+ YI G+ W+
Sbjct: 306 SGWQENREAGRD-KTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWL 336

Query: 377 LPPP 380
           LPPP
Sbjct: 366 LPPP 336

BLAST of Cp4.1LG18g02950 vs. ExPASy Swiss-Prot
Match: Q9LY19 (Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2)

HSP 1 Score: 344.4 bits (882), Expect = 1.7e-93
Identity = 172/366 (46.99%), Postives = 242/366 (66.12%), Query Frame = 0

Query: 17  VLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYRQRKANETGAKTLDPRL 76
           V  ++ ++ I +   +  +  T  IP    Q+ +W   ++    QRK        LDP L
Sbjct: 6   VSILLGMLVIFVSPMVFADDLT-PIPEGKPQVVQWFNTHVGPLAQRKG-------LDPAL 65

Query: 77  AKAEDCARIITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGGGTYREKITIDSSKPFV 136
             AE   RII V   G G F T+T AI+S+P+GN +RV++ +  G YREK+TID +KPF+
Sbjct: 66  VAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAHGEYREKVTIDRNKPFI 125

Query: 137 TLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFVNSAPMPDAGRTGGQAV 196
           TL GQ   MPVI+YDGTA+++GT +SA++ + SDYF+AVN+   N+AP PD    G QA+
Sbjct: 126 TLMGQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQAL 185

Query: 197 AMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTVINSV 256
           +MRISG+ AAF++C+F GFQDT+CDD G HFFKDCY+EGT DFIFG+G S+YL T ++ V
Sbjct: 186 SMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVV 245

Query: 257 AEGTGVITAQARENSTDDSGFTFAYCNITGT-GNTYLGRAWKERTRVVFAYTYMGTLINT 316
            +G  VI A A +++ + SG++F +C +TGT G  YLGRAW    +VV+AYT M +++N 
Sbjct: 246 GDGIRVIAAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNP 305

Query: 317 QGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDYEAKAFLSMTYIHGNKWV 376
            GW +        K+++YGEYKC GPG+    R  +T+ + D EA  FLS+ YI G+KW+
Sbjct: 306 TGWQENKTPAHD-KTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANCFLSLGYIQGSKWL 361

Query: 377 LPPPRL 382
           LPPP L
Sbjct: 366 LPPPAL 361

BLAST of Cp4.1LG18g02950 vs. ExPASy Swiss-Prot
Match: Q9LY18 (Probable pectinesterase 49 OS=Arabidopsis thaliana OX=3702 GN=PME49 PE=2 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 7.3e-92
Identity = 167/363 (46.01%), Postives = 244/363 (67.22%), Query Frame = 0

Query: 18  LAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYRQRKANETGAKTLDPRLA 77
           LA+V L+ +   + +V+      IPA+ +Q+ +W   N++ + QR+       TLDP L 
Sbjct: 6   LALVALL-VFFASPVVLADDITPIPADRAQIPQWFMANVKPFSQRRG------TLDPELE 65

Query: 78  KAEDCARIITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGGGTYREKITIDSSKPFVT 137
            AE   R+I V Q+G G+F TI  AI+SIP  N+ RV++ +  G Y EK+T+D  +P+VT
Sbjct: 66  AAEASRRVIIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTVDVGRPYVT 125

Query: 138 LYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFVNSAPMPDAGRTGGQAVA 197
           L G+ G    ++Y GTA+++GT ESAT+ V +  F+A N+  +N++PMP  G T GQA+A
Sbjct: 126 LLGKPGAETNLTYAGTAAKYGTVESATLIVWATNFLAANLNIINTSPMPKPG-TQGQALA 185

Query: 198 MRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTVINSVA 257
           MRI+GDKAAF++C+F GFQDTLCDDRG HFFK+CYIEGT DFIFG G SLYL T +++V 
Sbjct: 186 MRINGDKAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGASLYLTTQLHAVG 245

Query: 258 EGTGVITAQARENSTDDSGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINTQ 317
           +G  VI A  R+++T+ +G++F +C +TG G   YLGRAW    +VV++YT M +++N  
Sbjct: 246 DGLRVIAAHNRQSTTEQNGYSFVHCKVTGVGTGIYLGRAWMSHPKVVYSYTEMSSVVNPS 305

Query: 318 GWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDYEAKAFLSMTYIHGNKWVL 377
           GW +        K+++YGEY C GPG+    R  +T+ + + EA  FL++ YI G+KW+L
Sbjct: 306 GWQENRVRAHD-KTVFYGEYMCTGPGSHKAKRVAHTQDIDNKEASQFLTLGYIKGSKWLL 359

Query: 378 PPP 380
           PPP
Sbjct: 366 PPP 359

BLAST of Cp4.1LG18g02950 vs. NCBI nr
Match: XP_023515642.1 (putative pectinesterase 63 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 789 bits (2038), Expect = 4.02e-288
Identity = 392/392 (100.00%), Postives = 392/392 (100.00%), Query Frame = 0

Query: 1   MSRPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR 60
           MSRPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR
Sbjct: 1   MSRPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR 60

Query: 61  QRKANETGAKTLDPRLAKAEDCARIITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGG 120
           QRKANETGAKTLDPRLAKAEDCARIITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGG
Sbjct: 61  QRKANETGAKTLDPRLAKAEDCARIITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGG 120

Query: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV 180
           GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV
Sbjct: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV 180

Query: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240
           NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI
Sbjct: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240

Query: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT 300
           FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT 300

Query: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDYEA 360
           RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDYEA
Sbjct: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDYEA 360

Query: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 392
           KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG
Sbjct: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 392

BLAST of Cp4.1LG18g02950 vs. NCBI nr
Match: XP_022961183.1 (putative pectinesterase 63 [Cucurbita moschata] >KAG7023751.1 putative pectinesterase 50 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 785 bits (2026), Expect = 2.72e-286
Identity = 390/392 (99.49%), Postives = 391/392 (99.74%), Query Frame = 0

Query: 1   MSRPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR 60
           MSRPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR
Sbjct: 1   MSRPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR 60

Query: 61  QRKANETGAKTLDPRLAKAEDCARIITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGG 120
           QRKANETGAKTLDPRLAKAEDCAR+ITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGG
Sbjct: 61  QRKANETGAKTLDPRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGG 120

Query: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV 180
           GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV
Sbjct: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV 180

Query: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240
           NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI
Sbjct: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240

Query: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT 300
           FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT 300

Query: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDYEA 360
           RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTD EA
Sbjct: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360

Query: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 392
           KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG
Sbjct: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 392

BLAST of Cp4.1LG18g02950 vs. NCBI nr
Match: KAG6590056.1 (putative pectinesterase 50, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 780 bits (2014), Expect = 1.83e-284
Identity = 387/392 (98.72%), Postives = 390/392 (99.49%), Query Frame = 0

Query: 1   MSRPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR 60
           M+RPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR
Sbjct: 1   MARPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR 60

Query: 61  QRKANETGAKTLDPRLAKAEDCARIITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGG 120
           QRKANETGAKTLDPRLAKAEDCAR+ITVKQDGQGNFSTITQAI+SIPSGNRERVVVWIGG
Sbjct: 61  QRKANETGAKTLDPRLAKAEDCARLITVKQDGQGNFSTITQAIDSIPSGNRERVVVWIGG 120

Query: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV 180
           GTYREKITIDSSKPFVTLYGQ GKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV
Sbjct: 121 GTYREKITIDSSKPFVTLYGQIGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV 180

Query: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240
           NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI
Sbjct: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240

Query: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT 300
           FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT 300

Query: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDYEA 360
           RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTD EA
Sbjct: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360

Query: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 392
           KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG
Sbjct: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 392

BLAST of Cp4.1LG18g02950 vs. NCBI nr
Match: XP_022987214.1 (putative pectinesterase 63 [Cucurbita maxima])

HSP 1 Score: 768 bits (1983), Expect = 9.74e-280
Identity = 382/392 (97.45%), Postives = 385/392 (98.21%), Query Frame = 0

Query: 1   MSRPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR 60
           MSRPAVASSFPATVALVLAVVQLIT+LIPAAIVVEARTK IPAEPSQLDEWIAHNMREYR
Sbjct: 1   MSRPAVASSFPATVALVLAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYR 60

Query: 61  QRKANETGAKTLDPRLAKAEDCARIITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGG 120
           QRKA ETGAK LD RLAKAEDCAR+ITVKQDGQGNFSTITQAIESIPSGN ERVVVWIGG
Sbjct: 61  QRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGG 120

Query: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV 180
           GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGT ESATVAVESDYFVAVNVAFV
Sbjct: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFV 180

Query: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240
           NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI
Sbjct: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240

Query: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT 300
           FGNGKSLYLKTVINSVAEGTGVITAQARENSTD+SGFTFAYCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKERT 300

Query: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDYEA 360
           RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTD EA
Sbjct: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360

Query: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 392
           KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG
Sbjct: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 392

BLAST of Cp4.1LG18g02950 vs. NCBI nr
Match: XP_008450966.1 (PREDICTED: putative pectinesterase 63 [Cucumis melo])

HSP 1 Score: 649 bits (1673), Expect = 1.79e-232
Identity = 318/387 (82.17%), Postives = 348/387 (89.92%), Query Frame = 0

Query: 1   MSRPAVASSFPATVALVLAVV--QLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMRE 60
           M RP VASSF ATVAL LAV+   +I I IP+ ++VEARTKTIPA+ S+LDEWIAHNMRE
Sbjct: 1   MFRPTVASSFSATVALALAVMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60

Query: 61  YRQRKANETGAKTLDPRLAKAEDCARIITVKQDGQGNFSTITQAIESIPSGNRERVVVWI 120
           Y  RK NETG K LD RLA+AEDC R+ITV++DG+GNFSTIT+AIESIPSGNR RVVVWI
Sbjct: 61  YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120

Query: 121 GGGTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVA 180
           GGG YREKITID+SKPFVTLYGQ GK PVI++DGTASEFGT +SATVAVESDYFVAVN+ 
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180

Query: 181 FVNSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVD 240
           FVNSAPMP+ G TGGQAVAMRISGDKAAFH C FIGFQDTLCDDRGRHFF+DCYI+GTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240

Query: 241 FIFGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKT INSVAEG GVITAQARE++TD+SGFTFAYCNITGTGNTYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDY 360
           RTRVVFAYTYMGTLINT+GWSDKM G++PRKSMYYGEYKCKGPGA S+GR  Y RIL+D 
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360

Query: 361 EAKAFLSMTYIHGNKWVLPPPRLSLLP 385
           EAKAFLSMTYIHGNKW+LPPP LS  P
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSP 387

BLAST of Cp4.1LG18g02950 vs. ExPASy TrEMBL
Match: A0A6J1HB45 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111461773 PE=4 SV=1)

HSP 1 Score: 785 bits (2026), Expect = 1.32e-286
Identity = 390/392 (99.49%), Postives = 391/392 (99.74%), Query Frame = 0

Query: 1   MSRPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR 60
           MSRPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR
Sbjct: 1   MSRPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR 60

Query: 61  QRKANETGAKTLDPRLAKAEDCARIITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGG 120
           QRKANETGAKTLDPRLAKAEDCAR+ITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGG
Sbjct: 61  QRKANETGAKTLDPRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGG 120

Query: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV 180
           GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV
Sbjct: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV 180

Query: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240
           NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI
Sbjct: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240

Query: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT 300
           FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT 300

Query: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDYEA 360
           RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTD EA
Sbjct: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360

Query: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 392
           KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG
Sbjct: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 392

BLAST of Cp4.1LG18g02950 vs. ExPASy TrEMBL
Match: A0A6J1JI92 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111484834 PE=4 SV=1)

HSP 1 Score: 768 bits (1983), Expect = 4.72e-280
Identity = 382/392 (97.45%), Postives = 385/392 (98.21%), Query Frame = 0

Query: 1   MSRPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR 60
           MSRPAVASSFPATVALVLAVVQLIT+LIPAAIVVEARTK IPAEPSQLDEWIAHNMREYR
Sbjct: 1   MSRPAVASSFPATVALVLAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYR 60

Query: 61  QRKANETGAKTLDPRLAKAEDCARIITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGG 120
           QRKA ETGAK LD RLAKAEDCAR+ITVKQDGQGNFSTITQAIESIPSGN ERVVVWIGG
Sbjct: 61  QRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGG 120

Query: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV 180
           GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGT ESATVAVESDYFVAVNVAFV
Sbjct: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFV 180

Query: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240
           NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI
Sbjct: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240

Query: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT 300
           FGNGKSLYLKTVINSVAEGTGVITAQARENSTD+SGFTFAYCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKERT 300

Query: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDYEA 360
           RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTD EA
Sbjct: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360

Query: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 392
           KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG
Sbjct: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 392

BLAST of Cp4.1LG18g02950 vs. ExPASy TrEMBL
Match: A0A1S3BRI5 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492397 PE=4 SV=1)

HSP 1 Score: 649 bits (1673), Expect = 8.68e-233
Identity = 318/387 (82.17%), Postives = 348/387 (89.92%), Query Frame = 0

Query: 1   MSRPAVASSFPATVALVLAVV--QLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMRE 60
           M RP VASSF ATVAL LAV+   +I I IP+ ++VEARTKTIPA+ S+LDEWIAHNMRE
Sbjct: 1   MFRPTVASSFSATVALALAVMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60

Query: 61  YRQRKANETGAKTLDPRLAKAEDCARIITVKQDGQGNFSTITQAIESIPSGNRERVVVWI 120
           Y  RK NETG K LD RLA+AEDC R+ITV++DG+GNFSTIT+AIESIPSGNR RVVVWI
Sbjct: 61  YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120

Query: 121 GGGTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVA 180
           GGG YREKITID+SKPFVTLYGQ GK PVI++DGTASEFGT +SATVAVESDYFVAVN+ 
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180

Query: 181 FVNSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVD 240
           FVNSAPMP+ G TGGQAVAMRISGDKAAFH C FIGFQDTLCDDRGRHFF+DCYI+GTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240

Query: 241 FIFGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKT INSVAEG GVITAQARE++TD+SGFTFAYCNITGTGNTYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDY 360
           RTRVVFAYTYMGTLINT+GWSDKM G++PRKSMYYGEYKCKGPGA S+GR  Y RIL+D 
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360

Query: 361 EAKAFLSMTYIHGNKWVLPPPRLSLLP 385
           EAKAFLSMTYIHGNKW+LPPP LS  P
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSP 387

BLAST of Cp4.1LG18g02950 vs. ExPASy TrEMBL
Match: A0A0A0M299 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G597780 PE=4 SV=1)

HSP 1 Score: 642 bits (1656), Expect = 3.37e-230
Identity = 311/387 (80.36%), Postives = 347/387 (89.66%), Query Frame = 0

Query: 1   MSRPAVASSFPATVALVLAVVQ--LITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMRE 60
           M RP VASSFPATVA  + V+   +I I+IP+ +VVEA TKTIPA+ S+LDEWI HNM+E
Sbjct: 1   MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE 60

Query: 61  YRQRKANETGAKTLDPRLAKAEDCARIITVKQDGQGNFSTITQAIESIPSGNRERVVVWI 120
           Y  RK NETG K LD RLA+AEDC ++ITV++DG+GNFSTIT+AI+SIPSGNR RVVVWI
Sbjct: 61  YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI 120

Query: 121 GGGTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVA 180
           GGG YREKITID+SKPFVTLYGQ GK P+I++DGTASEFGT +SATVAVESDYFVAVN+ 
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT 180

Query: 181 FVNSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVD 240
           FVNSAPMP+ G TGGQAVAMRISGDKAAFH C FIGFQDTLCDDRGRHFFKDCY++GTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD 240

Query: 241 FIFGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKT INSVAEGTGVITAQARE++TD+SGFTFAYCNITGTG+TYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDY 360
           RTRVVFAYTYMGTLINT+GWSDKM G++PRKSMYYGEYKCKGPGA  +GR  Y RIL+D 
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV 360

Query: 361 EAKAFLSMTYIHGNKWVLPPPRLSLLP 385
           EAKAFLSMTYIHGNKW+LPPP LSL P
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSP 387

BLAST of Cp4.1LG18g02950 vs. ExPASy TrEMBL
Match: A0A6J1D0K0 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016481 PE=4 SV=1)

HSP 1 Score: 622 bits (1603), Expect = 3.29e-222
Identity = 309/382 (80.89%), Postives = 335/382 (87.70%), Query Frame = 0

Query: 1   MSRPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR 60
           M RPAVASS P  VA+ LAV+QLI IL PAA+V  ARTK IPAEPS+LDEWI +NM+EY 
Sbjct: 1   MYRPAVASSSPVAVAIALAVIQLIIIL-PAAVV--ARTKPIPAEPSKLDEWIENNMKEYH 60

Query: 61  QRKANETGAKTLDPRLAKAEDCARIITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGG 120
           +RKAN+TG K LD RLA AED  R+ITV+ DG+GNF+TIT AIESIP+GN  RV+VWI G
Sbjct: 61  ERKANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAG 120

Query: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV 180
           G YREKITID+SKPFVTLYGQ GKMPVI++DGTASEFGT +SATVAVESDYFVAVN+AFV
Sbjct: 121 GIYREKITIDASKPFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFV 180

Query: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240
           NSA MPD   +GGQAVAMRISGDKAAFHHC FIGFQDTLCDDRGRHFFKDCYIEGTVDFI
Sbjct: 181 NSAAMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240

Query: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT 300
           FGNGKSLYLKT INSVAEG GVITAQARE++TDDSGFTF YCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKERT 300

Query: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDYEA 360
           RVVFAYTYMG LINT+GWSDKM   EPRKSMYYGEYKC GPGA   GR  Y RIL+D EA
Sbjct: 301 RVVFAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEA 360

Query: 361 KAFLSMTYIHGNKWVLPPPRLS 382
           +AFLSMTYIHGNKW+LPPP LS
Sbjct: 361 RAFLSMTYIHGNKWLLPPPLLS 379

BLAST of Cp4.1LG18g02950 vs. TAIR 10
Match: AT5G07430.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 354.8 bits (909), Expect = 9.2e-98
Identity = 172/357 (48.18%), Postives = 246/357 (68.91%), Query Frame = 0

Query: 26  ILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYRQRKANETGAKTLDPRLAKAEDCARI 85
           ++  + +V+   T  IP   +Q+ +W   N++ Y QRK       TLDP L  AE   +I
Sbjct: 13  VVFASPVVLATDTDPIPENRAQIPQWFKTNVKPYSQRKG------TLDPALEAAEAARQI 72

Query: 86  ITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGGGTYREKITIDSSKPFVTLYGQTGKM 145
           ITV Q G  NF T+ +AI+SIP+GN+ RV++ +  G Y EK+TID ++PF+TL GQ G  
Sbjct: 73  ITVNQKGGANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTIDIARPFITLLGQPGAE 132

Query: 146 PVISYDGTASEFGTAESATVAVESDYFVAVNVAFVNSAPMPDAGRTGGQAVAMRISGDKA 205
            V++Y GTA+++GT ESAT+ V ++YF A ++   N+APMP  G + GQA+AMRI+ DKA
Sbjct: 133 TVLTYHGTAAQYGTVESATLIVWAEYFQAAHLTIKNTAPMPKPG-SQGQALAMRINADKA 192

Query: 206 AFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTVINSVAEGTGVITA 265
           AF+ C+F GFQDTLCDD+G HFFKDCYIEGT DFIFG G SLYL T +++V +G  VITA
Sbjct: 193 AFYSCRFHGFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGASLYLNTQLHAVGDGLRVITA 252

Query: 266 QARENSTDDSGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINTQGWSDKM-Q 325
           Q R+++T+ +G+TF +C +TGTG   YLGR+W    +VV+A+T M +++N  GW + + +
Sbjct: 253 QGRQSATEQNGYTFVHCKVTGTGTGIYLGRSWMSHPKVVYAFTEMTSVVNPSGWRENLNR 312

Query: 326 GTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDYEAKAFLSMTYIHGNKWVLPPPR 381
           G +  K+++YGEYKC GPG+    R  YT+ +   E   FL++ YI G+ W+LPPP+
Sbjct: 313 GYD--KTVFYGEYKCFGPGSHLEKRVPYTQDIDKNEVTPFLTLGYIKGSTWLLPPPK 360

BLAST of Cp4.1LG18g02950 vs. TAIR 10
Match: AT1G69940.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 350.1 bits (897), Expect = 2.3e-96
Identity = 169/342 (49.42%), Postives = 232/342 (67.84%), Query Frame = 0

Query: 41  IPAEPSQLDEWIAHNMREYRQRKANETGAKTLDPRLAKAEDCARIITVKQDGQGNFSTIT 100
           IP    Q+ +W   N+    QRK        LDP L  AE   RII V   G G F T+T
Sbjct: 29  IPEGKPQVAQWFNANVGPLAQRKG-------LDPALVAAEAAPRIINVNPKG-GEFKTLT 88

Query: 101 QAIESIPSGNRERVVVWIGGGTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTA 160
            AI+S+P+GN +RV++ +  G Y+EK+TID +KPF+TL GQ   MPVI+YDGTA+++GT 
Sbjct: 89  DAIKSVPAGNTKRVIIKMAPGEYKEKVTIDRNKPFITLMGQPNAMPVITYDGTAAKYGTV 148

Query: 161 ESATVAVESDYFVAVNVAFVNSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLC 220
           +SA++ + SDYF+AVN+   N+AP PD    G QA++MRISG+ AAF++C+F GFQDT+C
Sbjct: 149 DSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGNFAAFYNCKFYGFQDTIC 208

Query: 221 DDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFA 280
           DD G HFFKDCY+EGT DFIFG+G S+YL T ++ V +G  VI A A +++ + SG++F 
Sbjct: 209 DDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVGDGIRVIAAHAGKSAEEKSGYSFV 268

Query: 281 YCNITGT-GNTYLGRAWKERTRVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCK 340
           +C +TGT G  YLGRAW    +VV+AYT M +++N  GW +        K+++YGEYKC 
Sbjct: 269 HCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENKTPAHD-KTVFYGEYKCS 328

Query: 341 GPGADSTGRAHYTRILTDYEAKAFLSMTYIHGNKWVLPPPRL 382
           GPG+    R  +T+ + D EA  FLS+ YI G+KW+LPPP L
Sbjct: 329 GPGSHKAKRVPFTQDIDDKEANRFLSLGYIQGSKWLLPPPAL 361

BLAST of Cp4.1LG18g02950 vs. TAIR 10
Match: AT5G61680.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 349.4 bits (895), Expect = 3.9e-96
Identity = 169/364 (46.43%), Postives = 239/364 (65.66%), Query Frame = 0

Query: 17  VLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYRQRKANETGAKTLDPRL 76
           V  +V ++ ++I + +V       IP    ++++W   N                     
Sbjct: 6   VSLIVTILLVVITSPVVFGNDAAPIPENKGRIEQWFNTN--------------------- 65

Query: 77  AKAEDCARIITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGGGTYREKITIDSSKPFV 136
                      VKQ+G+G+F TIT+AI S+ +GN  RV++ IG G Y+EK+TID SKPF+
Sbjct: 66  -----------VKQNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFI 125

Query: 137 TLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFVNSAPMPDAGRTGGQAV 196
           TLYG    MPV+++DGTA+++GT +SAT+ V SDYF+AVN+   NSAPMPD  R G QA+
Sbjct: 126 TLYGHPNAMPVLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQAL 185

Query: 197 AMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTVINSV 256
           +MRISG+KAAF++C+F G+QDT+CDD G HFFKDCYIEGT DFIFG+G+SLYL T +N V
Sbjct: 186 SMRISGNKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVV 245

Query: 257 AEGTGVITAQARENSTDDSGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINT 316
            +G  VITA A +++ + SG++F +C +TGTG   YLGR+W    +VV+AYT M +++N 
Sbjct: 246 GDGIRVITAHAGKSAAEKSGYSFVHCKVTGTGTGIYLGRSWMSHPKVVYAYTDMSSVVNP 305

Query: 317 QGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDYEAKAFLSMTYIHGNKWV 376
            GW +  +     K+++YGEYKC G G+    R  YT+ + D EAK F+S+ YI G+ W+
Sbjct: 306 SGWQENREAGRD-KTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWL 336

Query: 377 LPPP 380
           LPPP
Sbjct: 366 LPPP 336

BLAST of Cp4.1LG18g02950 vs. TAIR 10
Match: AT5G07410.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 344.4 bits (882), Expect = 1.2e-94
Identity = 172/366 (46.99%), Postives = 242/366 (66.12%), Query Frame = 0

Query: 17  VLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYRQRKANETGAKTLDPRL 76
           V  ++ ++ I +   +  +  T  IP    Q+ +W   ++    QRK        LDP L
Sbjct: 6   VSILLGMLVIFVSPMVFADDLT-PIPEGKPQVVQWFNTHVGPLAQRKG-------LDPAL 65

Query: 77  AKAEDCARIITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGGGTYREKITIDSSKPFV 136
             AE   RII V   G G F T+T AI+S+P+GN +RV++ +  G YREK+TID +KPF+
Sbjct: 66  VAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAHGEYREKVTIDRNKPFI 125

Query: 137 TLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFVNSAPMPDAGRTGGQAV 196
           TL GQ   MPVI+YDGTA+++GT +SA++ + SDYF+AVN+   N+AP PD    G QA+
Sbjct: 126 TLMGQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQAL 185

Query: 197 AMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTVINSV 256
           +MRISG+ AAF++C+F GFQDT+CDD G HFFKDCY+EGT DFIFG+G S+YL T ++ V
Sbjct: 186 SMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVV 245

Query: 257 AEGTGVITAQARENSTDDSGFTFAYCNITGT-GNTYLGRAWKERTRVVFAYTYMGTLINT 316
            +G  VI A A +++ + SG++F +C +TGT G  YLGRAW    +VV+AYT M +++N 
Sbjct: 246 GDGIRVIAAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNP 305

Query: 317 QGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDYEAKAFLSMTYIHGNKWV 376
            GW +        K+++YGEYKC GPG+    R  +T+ + D EA  FLS+ YI G+KW+
Sbjct: 306 TGWQENKTPAHD-KTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANCFLSLGYIQGSKWL 361

Query: 377 LPPPRL 382
           LPPP L
Sbjct: 366 LPPPAL 361

BLAST of Cp4.1LG18g02950 vs. TAIR 10
Match: AT5G07420.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 339.0 bits (868), Expect = 5.2e-93
Identity = 167/363 (46.01%), Postives = 244/363 (67.22%), Query Frame = 0

Query: 18  LAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYRQRKANETGAKTLDPRLA 77
           LA+V L+ +   + +V+      IPA+ +Q+ +W   N++ + QR+       TLDP L 
Sbjct: 6   LALVALL-VFFASPVVLADDITPIPADRAQIPQWFMANVKPFSQRRG------TLDPELE 65

Query: 78  KAEDCARIITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGGGTYREKITIDSSKPFVT 137
            AE   R+I V Q+G G+F TI  AI+SIP  N+ RV++ +  G Y EK+T+D  +P+VT
Sbjct: 66  AAEASRRVIIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTVDVGRPYVT 125

Query: 138 LYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFVNSAPMPDAGRTGGQAVA 197
           L G+ G    ++Y GTA+++GT ESAT+ V +  F+A N+  +N++PMP  G T GQA+A
Sbjct: 126 LLGKPGAETNLTYAGTAAKYGTVESATLIVWATNFLAANLNIINTSPMPKPG-TQGQALA 185

Query: 198 MRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTVINSVA 257
           MRI+GDKAAF++C+F GFQDTLCDDRG HFFK+CYIEGT DFIFG G SLYL T +++V 
Sbjct: 186 MRINGDKAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGASLYLTTQLHAVG 245

Query: 258 EGTGVITAQARENSTDDSGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINTQ 317
           +G  VI A  R+++T+ +G++F +C +TG G   YLGRAW    +VV++YT M +++N  
Sbjct: 246 DGLRVIAAHNRQSTTEQNGYSFVHCKVTGVGTGIYLGRAWMSHPKVVYSYTEMSSVVNPS 305

Query: 318 GWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDYEAKAFLSMTYIHGNKWVL 377
           GW +        K+++YGEY C GPG+    R  +T+ + + EA  FL++ YI G+KW+L
Sbjct: 306 GWQENRVRAHD-KTVFYGEYMCTGPGSHKAKRVAHTQDIDNKEASQFLTLGYIKGSKWLL 359

Query: 378 PPP 380
           PPP
Sbjct: 366 PPP 359

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LY171.3e-9648.18Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1[more]
Q84WM73.2e-9549.42Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1[more]
Q9FKF35.4e-9546.43Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2[more]
Q9LY191.7e-9346.99Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2[more]
Q9LY187.3e-9246.01Probable pectinesterase 49 OS=Arabidopsis thaliana OX=3702 GN=PME49 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023515642.14.02e-288100.00putative pectinesterase 63 [Cucurbita pepo subsp. pepo][more]
XP_022961183.12.72e-28699.49putative pectinesterase 63 [Cucurbita moschata] >KAG7023751.1 putative pectinest... [more]
KAG6590056.11.83e-28498.72putative pectinesterase 50, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022987214.19.74e-28097.45putative pectinesterase 63 [Cucurbita maxima][more]
XP_008450966.11.79e-23282.17PREDICTED: putative pectinesterase 63 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1HB451.32e-28699.49Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111461773 PE=4 SV=1[more]
A0A6J1JI924.72e-28097.45Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111484834 PE=4 SV=1[more]
A0A1S3BRI58.68e-23382.17Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492397 PE=4 SV=1[more]
A0A0A0M2993.37e-23080.36Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G597780 PE=4 SV=1[more]
A0A6J1D0K03.29e-22280.89Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016481 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G07430.19.2e-9848.18Pectin lyase-like superfamily protein [more]
AT1G69940.12.3e-9649.42Pectin lyase-like superfamily protein [more]
AT5G61680.13.9e-9646.43Pectin lyase-like superfamily protein [more]
AT5G07410.11.2e-9446.99Pectin lyase-like superfamily protein [more]
AT5G07420.15.2e-9346.01Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 86..372
e-value: 1.2E-66
score: 224.5
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 79..377
e-value: 3.1E-101
score: 340.9
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 19..379
NoneNo IPR availablePANTHERPTHR31321:SF30PECTINESTERASEcoord: 19..379
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 233..242
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 83..375

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG18g02950.1Cp4.1LG18g02950.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity