Cp4.1LG18g02100 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG18g02100 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 3.5e-49
Identity = 146/447 (32.66%), Postives = 199/447 (44.52%), Query Frame = 0

Query: 2   DELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKASD 61
           +E+ +P YFICPISL+IMKDPVT  +GITYDR++I  W    +    CP+TKQPL   SD
Sbjct: 6   EEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL---EKVPSCPVTKQPLPLDSD 65

Query: 62  LTPNHTLHRLIKCWLVENATPGSGFDGILSPRPLLDKTHLRKLLRDLAA-SDDGL-RHDA 121
           LTPNH L RLI+ W VEN T   G   I +PR    K ++ + +++L     + L R + 
Sbjct: 66  LTPNHMLRRLIQHWCVENET--RGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREET 125

Query: 122 VRKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVG-----GLEE--------- 181
           ++KL  LA++G+   R  M E GV K ++LFV++C  E + G     GL+E         
Sbjct: 126 LQKLEVLAMDGNN--RRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIG 185

Query: 182 ------------------------------------------------------------ 241
                                                                       
Sbjct: 186 IPSNDAKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERLNPEIFK 245

Query: 242 ------------------------------------------------------------ 300
                                                                       
Sbjct: 246 GIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLET 305

BLAST of Cp4.1LG18g02100 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 4.0e-45
Identity = 128/399 (32.08%), Postives = 187/399 (46.87%), Query Frame = 0

Query: 2   DELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKASD 61
           +E+ +PP+F+CPISL+IMKDPV  +TGITYDR+SIE W    K    CP+TKQ +T A D
Sbjct: 8   EEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKN-SCPVTKQDITDA-D 67

Query: 62  LTPNHTLHRLIKCWLVENATPGSGFDGILSPRPLLDKTHLRKLLRDLAASDD-------- 121
           LTPNHTL RLI+ W   NA+   G + I +PRP + K+ + KL+RD A+S +        
Sbjct: 68  LTPNHTLRRLIQSWCTLNAS--YGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKR 127

Query: 122 -----------------------------------GLRHDAVRKLYALAIEGDTACRGRM 181
                                               L  +A+  LY L    +T  +  +
Sbjct: 128 LRQIVSENATNKRCLEAAGVPEFLANIVSNDSENGSLTDEALNLLYHLE-TSETVLKNLL 187

Query: 182 ---VEVGVAKGMVLFVMRCFKEGKVGG-------LE------------------------ 241
               +  + K +   + R   E +V         LE                        
Sbjct: 188 NNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDD 247

Query: 242 ----------------------------EVGAIFDLIALELEK---PEKNITELIFNLLA 293
                                       E G I  +I L +++    E+   E+   +L 
Sbjct: 248 RISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLD 307

BLAST of Cp4.1LG18g02100 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.9e-39
Identity = 122/427 (28.57%), Postives = 184/427 (43.09%), Query Frame = 0

Query: 3   ELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKASDL 62
           E+ +P +F+CPISL IMKDPV  +TGITYDRESIE W  + K    CP+TKQ +T+ +DL
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKN-SCPVTKQVITE-TDL 63

Query: 63  TPNHTLHRLIKCWLVENATPGSGFDGILSPRPLLDKTHLRKLLRDLAASDDGLRHDAVRK 122
           TPNHTL RLI+ W   NA+   G + I +P+P + K+ + KL+++ ++S    +   +++
Sbjct: 64  TPNHTLRRLIQSWCTLNAS--YGIERIPTPKPPICKSEIEKLIKESSSSHLN-QVKCLKR 123

Query: 123 LYALAIEGDT---------------------------------------ACRGRMVE--- 182
           L  +  E  T                                        C+  M+E   
Sbjct: 124 LRQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRF 183

Query: 183 ---------------------------------VGVAKGMVLFVMRCFKEGK------VG 242
                                              + K +   + R   E +      + 
Sbjct: 184 DSSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLK 243

Query: 243 GLEEVGAIFDLIALELE------------------------------------------- 293
            L EV     +I LE E                                           
Sbjct: 244 KLLEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGT 303

BLAST of Cp4.1LG18g02100 vs. ExPASy Swiss-Prot
Match: Q9C8D1 (U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.2e-17
Identity = 68/209 (32.54%), Postives = 98/209 (46.89%), Query Frame = 0

Query: 3   ELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKASDL 62
           E+ +P  F CPIS ++MKDPV   +GITYDRE+IE WF +      CP+T   LT    +
Sbjct: 30  EITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQT--CPVTNTVLTSLEQI 89

Query: 63  TPNHTLHRLIKCWLVENATPGSGFDGILSPRPLLDKTHLRKLLRDLAAS----DDGLRHD 122
            PNHT+ R+I+ W    ++ G G + I +PR  +    + ++   L+A+    D     +
Sbjct: 90  -PNHTIRRMIQGWC--GSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACME 149

Query: 123 AVRKLYALAIEGDTACRGRMVEVGVAKGMVLFV-MRCFKEGKVGGL---EEVGAIFDLIA 182
            V K+  L  E +   R R        G+VL V    F E     L   E V  +  ++ 
Sbjct: 150 MVTKMTRLGKESE---RNRKCVKENGAGLVLCVCFDAFSENANASLLLEETVSVLTWMLP 209

Query: 183 LELEKPEKNITELIFNLLAHLCTTADGRA 204
           + LE   K  T   FN L  L    D  A
Sbjct: 210 IGLEGQSKLTTTSSFNRLVELLRNGDQNA 230

BLAST of Cp4.1LG18g02100 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 2.1e-17
Identity = 48/107 (44.86%), Postives = 68/107 (63.55%), Query Frame = 0

Query: 4   LAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKASDLT 63
           + +P +F CPISL++M+DPVT  TG TYDR SIE+W     +   CP+T+ PL+  + L 
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTT-CPVTRAPLSDFT-LI 71

Query: 64  PNHTLHRLIKCWLVENATPGSGFDGILSPRPLLDKTHLRKLLRDLAA 111
           PNHTL RLI+ W V N +  +G + I +P+   D T +R LL   +A
Sbjct: 72  PNHTLRRLIQEWCVANRS--NGVERIPTPKQPADPTSVRALLSQASA 114

BLAST of Cp4.1LG18g02100 vs. NCBI nr
Match: XP_023516826.1 (E3 ubiquitin-protein ligase PUB24-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 553 bits (1425), Expect = 6.13e-196
Identity = 302/415 (72.77%), Postives = 302/415 (72.77%), Query Frame = 0

Query: 1   MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKAS 60
           MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKAS
Sbjct: 1   MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKAS 60

Query: 61  DLTPNHTLHRLIKCWLVENATPGSGFDGILSPRPLLDKTHLRKLLRDLAASDDGLRHDAV 120
           DLTPNHTLHRLIKCWLVENATPGSGFDGILSPRPLLDKTHLRKLLRDLAASDDGLRHDAV
Sbjct: 61  DLTPNHTLHRLIKCWLVENATPGSGFDGILSPRPLLDKTHLRKLLRDLAASDDGLRHDAV 120

Query: 121 RKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEE--------------- 180
           RKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEE               
Sbjct: 121 RKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEEGLKILSVLESYSICE 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 TRVLDVIDSLNWVLGLEMENHIIIKSYAIEVLKKAIDVAPSTVISGYEPLCRGFESLQFF 240

Query: 241 --------------------------------------VGAIFDLIALELEKPEKNITEL 300
                                                 VGAIFDLIALELEKPEKNITEL
Sbjct: 241 KNVTNFLRQKISNSALKTTLTILTKLCHWGRNRIKLVEVGAIFDLIALELEKPEKNITEL 300

Query: 301 IFNLLAHLCTTADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVL 302
           IFNLLAHLCTTADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVL
Sbjct: 301 IFNLLAHLCTTADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVL 360

BLAST of Cp4.1LG18g02100 vs. NCBI nr
Match: XP_022961078.1 (E3 ubiquitin-protein ligase PUB24-like [Cucurbita moschata] >KAG7023800.1 E3 ubiquitin-protein ligase PUB24, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 542 bits (1396), Expect = 1.59e-191
Identity = 297/415 (71.57%), Postives = 298/415 (71.81%), Query Frame = 0

Query: 1   MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKAS 60
           MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKAS
Sbjct: 1   MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKAS 60

Query: 61  DLTPNHTLHRLIKCWLVENATPGSGFDGILSPRPLLDKTHLRKLLRDLAASDDGLRHDAV 120
           DLTPNHTLHRLIK WLVENATPGSGFD ILSPRPLLDKTHLRKLLRDL ASDDGLRHDAV
Sbjct: 61  DLTPNHTLHRLIKSWLVENATPGSGFDRILSPRPLLDKTHLRKLLRDLTASDDGLRHDAV 120

Query: 121 RKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEE--------------- 180
           RKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEE               
Sbjct: 121 RKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEEGLKILSVLESYSICE 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 TRVLDVIDSLNWVLGLEMENHIIIKSYAIEVLKKAIDAAPSTVISGYEPRYQGFESLQFF 240

Query: 241 --------------------------------------VGAIFDLIALELEKPEKNITEL 300
                                                 VGA+FDLIALELEKPEKNITEL
Sbjct: 241 KNVTNLLREKISNSALKTTLTILTKLCHWGRNRIKLVEVGAVFDLIALELEKPEKNITEL 300

Query: 301 IFNLLAHLCTTADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVL 302
           IFNLLAHLCT ADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVL
Sbjct: 301 IFNLLAHLCTMADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVL 360

BLAST of Cp4.1LG18g02100 vs. NCBI nr
Match: XP_022987475.1 (E3 ubiquitin-protein ligase PUB24-like [Cucurbita maxima])

HSP 1 Score: 537 bits (1384), Expect = 1.37e-189
Identity = 295/422 (69.91%), Postives = 299/422 (70.85%), Query Frame = 0

Query: 1   MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKAS 60
           MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWF+TSKDAIFCPLTKQPLTKAS
Sbjct: 1   MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQPLTKAS 60

Query: 61  DLTPNHTLHRLIKCWLVENATPGSGFDGILSPRPLLDKTHLRKLLRDLAASDDGLRHDAV 120
           DLTPNHTLHRLIK WLVENATPGSGFD ILSPRPLLDKTHLRKLLRDL ASDDGLRHDAV
Sbjct: 61  DLTPNHTLHRLIKSWLVENATPGSGFDRILSPRPLLDKTHLRKLLRDLTASDDGLRHDAV 120

Query: 121 RKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEE--------------- 180
           RKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEE               
Sbjct: 121 RKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEEGLKILSVLESYSICE 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 TRVLDVIDSLNWVLGLEMENHIIIKSYAIEVLKKAIDAAPSTVISGYELRYHEFESRYQG 240

Query: 241 ---------------------------------------------VGAIFDLIALELEKP 300
                                                        VGA+F+LIALELEKP
Sbjct: 241 FESLQFFKNVTNLLREKISNSALKTTLTILTKLCHWGRNRIKLVEVGAVFNLIALELEKP 300

Query: 301 EKNITELIFNLLAHLCTTADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKH 302
           EKNITELIFNLLAHLCTTADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKH
Sbjct: 301 EKNITELIFNLLAHLCTTADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKH 360

BLAST of Cp4.1LG18g02100 vs. NCBI nr
Match: KAG6590133.1 (E3 ubiquitin-protein ligase PUB24, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 506 bits (1302), Expect = 1.68e-177
Identity = 279/397 (70.28%), Postives = 280/397 (70.53%), Query Frame = 0

Query: 19  MKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKASDLTPNHTLHRLIKCWLVE 78
           MKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKASDLTPNHTLHRLIK WLVE
Sbjct: 1   MKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKASDLTPNHTLHRLIKSWLVE 60

Query: 79  NATPGSGFDGILSPRPLLDKTHLRKLLRDLAASDDGLRHDAVRKLYALAIEGDTACRGRM 138
           NATPGSGFD ILSPRPLLDKTHLRKLLRDL ASDDGLRHDAVRKLYALAIEGDTACRGRM
Sbjct: 61  NATPGSGFDRILSPRPLLDKTHLRKLLRDLTASDDGLRHDAVRKLYALAIEGDTACRGRM 120

Query: 139 VEVGVAKGMVLFVMRCFKEGKVGGLEE--------------------------------- 198
           VEVGVAKGMVLFVMRCFKEGKVGGLEE                                 
Sbjct: 121 VEVGVAKGMVLFVMRCFKEGKVGGLEEGLKILSVLESYSICETRVLDVIDSLNWVLGLEM 180

Query: 199 ------------------------------------------------------------ 258
                                                                       
Sbjct: 181 ENHIIIKSYAIEVLKKAIDAAPSTVISGYEPRYQGFESLQFFKNVTNLLREKISNSALKT 240

Query: 259 --------------------VGAIFDLIALELEKPEKNITELIFNLLAHLCTTADGRAEL 302
                               VGA+FDLIALELEKPEKNITELIFNLLAHLCT ADGRAEL
Sbjct: 241 TLTILTKLCHWGRNRIKLVEVGAVFDLIALELEKPEKNITELIFNLLAHLCTMADGRAEL 300

BLAST of Cp4.1LG18g02100 vs. NCBI nr
Match: XP_008450468.1 (PREDICTED: E3 ubiquitin-protein ligase PUB24-like [Cucumis melo] >KAA0040216.1 E3 ubiquitin-protein ligase PUB24-like [Cucumis melo var. makuwa] >TYK07878.1 E3 ubiquitin-protein ligase PUB24-like [Cucumis melo var. makuwa])

HSP 1 Score: 474 bits (1221), Expect = 5.18e-165
Identity = 262/408 (64.22%), Postives = 285/408 (69.85%), Query Frame = 0

Query: 1   MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKAS 60
           M+E+AVPPYFICPISLQIMKDPVTATTGITYDRESIENWF+TSKDAIFCPLTKQ LTKAS
Sbjct: 1   MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS 60

Query: 61  DLTPNHTLHRLIKCWLVENATPGSGFDGILSPRPLLDKTHLRKLLRDLA-ASDDGLRHDA 120
           DLTPNHTL RLIK W+VENA+PG G D  L+P PLLDKT LRKLLR++A ASD+ LR DA
Sbjct: 61  DLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDA 120

Query: 121 VRKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEE-------------- 180
           V+KL+ALAIE DTA RGRM EVGVAKGMVLFV+R F+EG++GGLEE              
Sbjct: 121 VKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSIS 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 EIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQINLEFFKNITNLF 240

Query: 241 -------------------------------VGAIFDLIALELEKPEKNITELIFNLLAH 300
                                          VGA+FDLI LELEKPEKNITELIFNLLAH
Sbjct: 241 REKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNITELIFNLLAH 300

Query: 301 LCTTADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLVEMLRVG 302
           LC+TADGRAEL+ HAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVL+EMLRVG
Sbjct: 301 LCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRVG 360

BLAST of Cp4.1LG18g02100 vs. ExPASy TrEMBL
Match: A0A6J1HAU5 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461696 PE=4 SV=1)

HSP 1 Score: 542 bits (1396), Expect = 7.70e-192
Identity = 297/415 (71.57%), Postives = 298/415 (71.81%), Query Frame = 0

Query: 1   MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKAS 60
           MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKAS
Sbjct: 1   MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKAS 60

Query: 61  DLTPNHTLHRLIKCWLVENATPGSGFDGILSPRPLLDKTHLRKLLRDLAASDDGLRHDAV 120
           DLTPNHTLHRLIK WLVENATPGSGFD ILSPRPLLDKTHLRKLLRDL ASDDGLRHDAV
Sbjct: 61  DLTPNHTLHRLIKSWLVENATPGSGFDRILSPRPLLDKTHLRKLLRDLTASDDGLRHDAV 120

Query: 121 RKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEE--------------- 180
           RKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEE               
Sbjct: 121 RKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEEGLKILSVLESYSICE 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 TRVLDVIDSLNWVLGLEMENHIIIKSYAIEVLKKAIDAAPSTVISGYEPRYQGFESLQFF 240

Query: 241 --------------------------------------VGAIFDLIALELEKPEKNITEL 300
                                                 VGA+FDLIALELEKPEKNITEL
Sbjct: 241 KNVTNLLREKISNSALKTTLTILTKLCHWGRNRIKLVEVGAVFDLIALELEKPEKNITEL 300

Query: 301 IFNLLAHLCTTADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVL 302
           IFNLLAHLCT ADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVL
Sbjct: 301 IFNLLAHLCTMADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVL 360

BLAST of Cp4.1LG18g02100 vs. ExPASy TrEMBL
Match: A0A6J1JGZ2 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485014 PE=4 SV=1)

HSP 1 Score: 537 bits (1384), Expect = 6.62e-190
Identity = 295/422 (69.91%), Postives = 299/422 (70.85%), Query Frame = 0

Query: 1   MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKAS 60
           MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWF+TSKDAIFCPLTKQPLTKAS
Sbjct: 1   MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQPLTKAS 60

Query: 61  DLTPNHTLHRLIKCWLVENATPGSGFDGILSPRPLLDKTHLRKLLRDLAASDDGLRHDAV 120
           DLTPNHTLHRLIK WLVENATPGSGFD ILSPRPLLDKTHLRKLLRDL ASDDGLRHDAV
Sbjct: 61  DLTPNHTLHRLIKSWLVENATPGSGFDRILSPRPLLDKTHLRKLLRDLTASDDGLRHDAV 120

Query: 121 RKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEE--------------- 180
           RKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEE               
Sbjct: 121 RKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEEGLKILSVLESYSICE 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 TRVLDVIDSLNWVLGLEMENHIIIKSYAIEVLKKAIDAAPSTVISGYELRYHEFESRYQG 240

Query: 241 ---------------------------------------------VGAIFDLIALELEKP 300
                                                        VGA+F+LIALELEKP
Sbjct: 241 FESLQFFKNVTNLLREKISNSALKTTLTILTKLCHWGRNRIKLVEVGAVFNLIALELEKP 300

Query: 301 EKNITELIFNLLAHLCTTADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKH 302
           EKNITELIFNLLAHLCTTADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKH
Sbjct: 301 EKNITELIFNLLAHLCTTADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKH 360

BLAST of Cp4.1LG18g02100 vs. ExPASy TrEMBL
Match: A0A5A7TDP8 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1933G00240 PE=4 SV=1)

HSP 1 Score: 474 bits (1221), Expect = 2.51e-165
Identity = 262/408 (64.22%), Postives = 285/408 (69.85%), Query Frame = 0

Query: 1   MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKAS 60
           M+E+AVPPYFICPISLQIMKDPVTATTGITYDRESIENWF+TSKDAIFCPLTKQ LTKAS
Sbjct: 1   MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS 60

Query: 61  DLTPNHTLHRLIKCWLVENATPGSGFDGILSPRPLLDKTHLRKLLRDLA-ASDDGLRHDA 120
           DLTPNHTL RLIK W+VENA+PG G D  L+P PLLDKT LRKLLR++A ASD+ LR DA
Sbjct: 61  DLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDA 120

Query: 121 VRKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEE-------------- 180
           V+KL+ALAIE DTA RGRM EVGVAKGMVLFV+R F+EG++GGLEE              
Sbjct: 121 VKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSIS 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 EIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQINLEFFKNITNLF 240

Query: 241 -------------------------------VGAIFDLIALELEKPEKNITELIFNLLAH 300
                                          VGA+FDLI LELEKPEKNITELIFNLLAH
Sbjct: 241 REKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNITELIFNLLAH 300

Query: 301 LCTTADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLVEMLRVG 302
           LC+TADGRAEL+ HAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVL+EMLRVG
Sbjct: 301 LCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRVG 360

BLAST of Cp4.1LG18g02100 vs. ExPASy TrEMBL
Match: A0A1S3BP96 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103492061 PE=4 SV=1)

HSP 1 Score: 474 bits (1221), Expect = 2.51e-165
Identity = 262/408 (64.22%), Postives = 285/408 (69.85%), Query Frame = 0

Query: 1   MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKAS 60
           M+E+AVPPYFICPISLQIMKDPVTATTGITYDRESIENWF+TSKDAIFCPLTKQ LTKAS
Sbjct: 1   MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS 60

Query: 61  DLTPNHTLHRLIKCWLVENATPGSGFDGILSPRPLLDKTHLRKLLRDLA-ASDDGLRHDA 120
           DLTPNHTL RLIK W+VENA+PG G D  L+P PLLDKT LRKLLR++A ASD+ LR DA
Sbjct: 61  DLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDA 120

Query: 121 VRKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEE-------------- 180
           V+KL+ALAIE DTA RGRM EVGVAKGMVLFV+R F+EG++GGLEE              
Sbjct: 121 VKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSIS 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 EIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQINLEFFKNITNLF 240

Query: 241 -------------------------------VGAIFDLIALELEKPEKNITELIFNLLAH 300
                                          VGA+FDLI LELEKPEKNITELIFNLLAH
Sbjct: 241 REKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNITELIFNLLAH 300

Query: 301 LCTTADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLVEMLRVG 302
           LC+TADGRAEL+ HAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVL+EMLRVG
Sbjct: 301 LCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRVG 360

BLAST of Cp4.1LG18g02100 vs. ExPASy TrEMBL
Match: A0A0A0M309 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G633420 PE=4 SV=1)

HSP 1 Score: 468 bits (1205), Expect = 6.79e-163
Identity = 261/408 (63.97%), Postives = 284/408 (69.61%), Query Frame = 0

Query: 1   MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKD-AIFCPLTKQPLTKA 60
           M+E AVPPYFICPISLQIMKDPVTATTGITYDRESI+NWF+TSKD AIFCPLTKQ LTKA
Sbjct: 1   MEEAAVPPYFICPISLQIMKDPVTATTGITYDRESIQNWFLTSKDDAIFCPLTKQSLTKA 60

Query: 61  SDLTPNHTLHRLIKCWLVENATPGSGFDGILSPRPLLDKTHLRKLLRDLAASDDGLRHDA 120
           SDLTPNHTL RLIK W+VENA+   G D IL+PRPLLDKT LRK+LR++AASD+ LR D+
Sbjct: 61  SDLTPNHTLRRLIKSWMVENASSAGGDDQILTPRPLLDKTCLRKILREVAASDERLRIDS 120

Query: 121 VRKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEE-------------- 180
           V+KL+ALAIE DTA RGRM EVGVAKGMVLFV+R F+EG+VGGLEE              
Sbjct: 121 VKKLHALAIESDTANRGRMEEVGVAKGMVLFVIRRFREGRVGGLEEALKILSLLSHYSIS 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 ETRVLDFFESLTWVLGLEMENHIIIKSYAIEVLKKATDVAPSTILTQINIEFFKNITNLL 240

Query: 241 -------------------------------VGAIFDLIALELEKPEKNITELIFNLLAH 300
                                          VGA+FDLIALELEKPEK  TELIFNLLAH
Sbjct: 241 REKISNSSLKTTLTILTNICPCGRNRVKLVEVGAVFDLIALELEKPEKKTTELIFNLLAH 300

Query: 301 LCTTADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLVEMLRVG 302
           LC+TADGRAEL+RHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVL+EMLRVG
Sbjct: 301 LCSTADGRAELIRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRVG 360

BLAST of Cp4.1LG18g02100 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 196.8 bits (499), Expect = 2.5e-50
Identity = 146/447 (32.66%), Postives = 199/447 (44.52%), Query Frame = 0

Query: 2   DELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKASD 61
           +E+ +P YFICPISL+IMKDPVT  +GITYDR++I  W    +    CP+TKQPL   SD
Sbjct: 20  EEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL---EKVPSCPVTKQPLPLDSD 79

Query: 62  LTPNHTLHRLIKCWLVENATPGSGFDGILSPRPLLDKTHLRKLLRDLAA-SDDGL-RHDA 121
           LTPNH L RLI+ W VEN T   G   I +PR    K ++ + +++L     + L R + 
Sbjct: 80  LTPNHMLRRLIQHWCVENET--RGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREET 139

Query: 122 VRKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVG-----GLEE--------- 181
           ++KL  LA++G+   R  M E GV K ++LFV++C  E + G     GL+E         
Sbjct: 140 LQKLEVLAMDGNN--RRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIG 199

Query: 182 ------------------------------------------------------------ 241
                                                                       
Sbjct: 200 IPSNDAKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERLNPEIFK 259

Query: 242 ------------------------------------------------------------ 300
                                                                       
Sbjct: 260 GIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLET 319

BLAST of Cp4.1LG18g02100 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 183.3 bits (464), Expect = 2.8e-46
Identity = 128/399 (32.08%), Postives = 187/399 (46.87%), Query Frame = 0

Query: 2   DELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKASD 61
           +E+ +PP+F+CPISL+IMKDPV  +TGITYDR+SIE W    K    CP+TKQ +T A D
Sbjct: 8   EEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKN-SCPVTKQDITDA-D 67

Query: 62  LTPNHTLHRLIKCWLVENATPGSGFDGILSPRPLLDKTHLRKLLRDLAASDD-------- 121
           LTPNHTL RLI+ W   NA+   G + I +PRP + K+ + KL+RD A+S +        
Sbjct: 68  LTPNHTLRRLIQSWCTLNAS--YGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKR 127

Query: 122 -----------------------------------GLRHDAVRKLYALAIEGDTACRGRM 181
                                               L  +A+  LY L    +T  +  +
Sbjct: 128 LRQIVSENATNKRCLEAAGVPEFLANIVSNDSENGSLTDEALNLLYHLE-TSETVLKNLL 187

Query: 182 ---VEVGVAKGMVLFVMRCFKEGKVGG-------LE------------------------ 241
               +  + K +   + R   E +V         LE                        
Sbjct: 188 NNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDD 247

Query: 242 ----------------------------EVGAIFDLIALELEK---PEKNITELIFNLLA 293
                                       E G I  +I L +++    E+   E+   +L 
Sbjct: 248 RISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLD 307

BLAST of Cp4.1LG18g02100 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 164.5 bits (415), Expect = 1.4e-40
Identity = 122/427 (28.57%), Postives = 184/427 (43.09%), Query Frame = 0

Query: 3   ELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKASDL 62
           E+ +P +F+CPISL IMKDPV  +TGITYDRESIE W  + K    CP+TKQ +T+ +DL
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKN-SCPVTKQVITE-TDL 63

Query: 63  TPNHTLHRLIKCWLVENATPGSGFDGILSPRPLLDKTHLRKLLRDLAASDDGLRHDAVRK 122
           TPNHTL RLI+ W   NA+   G + I +P+P + K+ + KL+++ ++S    +   +++
Sbjct: 64  TPNHTLRRLIQSWCTLNAS--YGIERIPTPKPPICKSEIEKLIKESSSSHLN-QVKCLKR 123

Query: 123 LYALAIEGDT---------------------------------------ACRGRMVE--- 182
           L  +  E  T                                        C+  M+E   
Sbjct: 124 LRQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRF 183

Query: 183 ---------------------------------VGVAKGMVLFVMRCFKEGK------VG 242
                                              + K +   + R   E +      + 
Sbjct: 184 DSSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLK 243

Query: 243 GLEEVGAIFDLIALELE------------------------------------------- 293
            L EV     +I LE E                                           
Sbjct: 244 KLLEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGT 303

BLAST of Cp4.1LG18g02100 vs. TAIR 10
Match: AT1G66160.1 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 92.0 bits (227), Expect = 8.5e-19
Identity = 68/209 (32.54%), Postives = 98/209 (46.89%), Query Frame = 0

Query: 3   ELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKASDL 62
           E+ +P  F CPIS ++MKDPV   +GITYDRE+IE WF +      CP+T   LT    +
Sbjct: 30  EITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQT--CPVTNTVLTSLEQI 89

Query: 63  TPNHTLHRLIKCWLVENATPGSGFDGILSPRPLLDKTHLRKLLRDLAAS----DDGLRHD 122
            PNHT+ R+I+ W    ++ G G + I +PR  +    + ++   L+A+    D     +
Sbjct: 90  -PNHTIRRMIQGWC--GSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACME 149

Query: 123 AVRKLYALAIEGDTACRGRMVEVGVAKGMVLFV-MRCFKEGKVGGL---EEVGAIFDLIA 182
            V K+  L  E +   R R        G+VL V    F E     L   E V  +  ++ 
Sbjct: 150 MVTKMTRLGKESE---RNRKCVKENGAGLVLCVCFDAFSENANASLLLEETVSVLTWMLP 209

Query: 183 LELEKPEKNITELIFNLLAHLCTTADGRA 204
           + LE   K  T   FN L  L    D  A
Sbjct: 210 IGLEGQSKLTTTSSFNRLVELLRNGDQNA 230

BLAST of Cp4.1LG18g02100 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 91.3 bits (225), Expect = 1.5e-18
Identity = 48/107 (44.86%), Postives = 68/107 (63.55%), Query Frame = 0

Query: 4   LAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKASDLT 63
           + +P +F CPISL++M+DPVT  TG TYDR SIE+W     +   CP+T+ PL+  + L 
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTT-CPVTRAPLSDFT-LI 71

Query: 64  PNHTLHRLIKCWLVENATPGSGFDGILSPRPLLDKTHLRKLLRDLAA 111
           PNHTL RLI+ W V N +  +G + I +P+   D T +R LL   +A
Sbjct: 72  PNHTLRRLIQEWCVANRS--NGVERIPTPKQPADPTSVRALLSQASA 114