Cp4.1LG16g08850 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG16g08850
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptiontRNA-5-taurinomethyluridine 2-sulfurtransferase
LocationCp4.1LG16: 8341443 .. 8346358 (+)
RNA-Seq ExpressionCp4.1LG16g08850
SyntenyCp4.1LG16g08850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTATTCGGCTCGTCTTCCTTTCGGAGCAAGAAGAATGCCGCGGCGGTGGATATAAGGAGGAATCAATTACAGTGATGCTGAGACTGAGAGTGAAGCCCGTTTGGTGCACTCTTCCGATCTCCCATTTCACTTCTCTTCCCCTTCGCCCCTCAATTCTCAGACCTGTTCTCCGCCGCCACAGGCCTCGACTCTTCCTCACCCGATTCTCCGCCTCCTCTGCTGTGCATAAAGCCTCGGAATCTTATTCAGCTAAAGGCCTCTCGGATGGCGACTTGGAGCCTTTCCTCTCATGTTCGATGCCTCGCTATCCGCTCAAAGTTGCTGTTCTTCTTAGCGGTGGCGTCGATAGCAGCGTCGCCCTCCGCCTCCTCCACGCTGCCGGCCACTCCTGCACTGCCTTCTATCTCAAGATATGGTTTCAGGTGCGTACTCTTTGTTCCTTTAATGGAACAGATCATATGCTTCATGTGTTTCCTTGTAAAGTAAGGAAGCTGATATTGGGAATGCTGGCGCAATCGGTGTTTAGGATTATAATTAATGACGAGCGTGGACGTGTTTCTGCTTTGCTATGTATATGATATGTATGTTCTTGGGCAGAGGACAATAGCCTTTTCATGTNCCAGTCACTCTTCAGGACAGTGATCCAGATGGACACGAAATGGGCTTCTGAAATGGCCCATATATCAGTTATCGTCAATTTCATTATTTTCATTTAAAATTTCAACGAAATTAATTTGGATAATATGTTATTTTAGTTTATTCGGCTCGTCTTCCTTTCGGAGCAAGAAGAATGCCGCGGCGGTGGATATAAGGAGGAATCAATTACAGTGATGCTGAGACTGAGAGTGAAGCCCGTTTGGTGCACTCTTCCGATCTCCCATTTCACTTCTCTTCCCCTTCGCCCCTCAATTCTCAGACCTGTTCTCCGCCGCCACAGGCCTCGACTCTTCCTCACCCGATTCTCCGCCTCCTCTGCTGTGCATAAAGCCTCGGAATCTTATTCAGCTAAAGGCCTCTCGGATGGCGACTTGGAGCCTTTCCTCTCATGTTCGATGCCTCGCTATCCGCTCAAAGTTGCTGTTCTTCTTAGCGGTGGCGTCGATAGCAGCGTCGCCCTCCGCCTCCTCCACGCTGCCGGCCACTCCTGCACTGCCTTCTATCTCAAGATATGGTTTCAGGTGCGTACTCTTTGTTCCTTTAATGGAACAGATCATATGCTTCATGTGTTTCCTTGTAAAGTAAGGAAGCTGATATTGGGAATGCTGGCGCAATCGGTGTTTAGGATTATAATTAATGACGAGCGTGGACGTGTTTCTGCTTTGCTATGTATATGATATGTATGTTCTTGGGCAGAGGACAATAGCCTTTTCATGTGACCGCAGTTTTGATGGATTTTGTTTGCGTAGGAAGACTTCGAGAATTTTTGGTCAGAATGCCCATGGGACGATGATTTGAAGTATGCTGAAGCTGTTTGTGATCAGGTTTGTCACTATTTTAGCTTTATGAATCCATCTAGACTTGCAGAGTTTTGAGATTGTTCTGATTGACTTTCTAAGGTCTTAAAAACACTTATTTTTGCATTTAGAAGCACTTTTTTTAAGTGCTAGGAAGTTAATCCAAACAGACTTTTAATTTGGCAAAAAAAAAAAAAAAAAAAAAAANAAACATCCAAATTGAACATTGTTCAAGATACCTATAAGTTATTTCGGCACATGATGCTAAGCGTAGTTATAGGTTCTGCGTGTGGGGAGAATCTGTCTTATTTTCTGCCGTCGTTTGTGCTGAAGAGAGAATTAGAACTGAAAATGTACACTGGTTTTCAGGTTGATGTACCATTGGAAGTGGTTCATTTGACAGAGGAATATTGGAGGAACGTGGTAAGTGCCTTTACAACTACAAAATTTTATTCTATAACTTTGTTGGATAACTTAATGATGTACTACATCAAACCAATTTGATTATGGTTGTGCTGGCTGACCATCTTGCTCATGTGTTCTTTCTAACAGGACGAAATAGTTTTGGGTTAGTTTTTCTGTCTGATGAATATTGTTTAGTTCATGTTTTTAGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGTACTCCAAACCCTGATGTCCTTTGTAACACAAGAATAAAGTTTGGTATGTTCATTAAATTTACTTGAATGGACTTCATAATTTTTTTTTTGCTATATTGTCAAGTTTTTGGTTATGTCTATAATGTTTTTTGTATTAATATAATTTAAGGCATTCCCTGCTTTTTCCAGGTGCATTCATGGATGCAATAAGCAATATGGAGTTTGATTATGTTGCTTCAGGGCATTATGCGAATGTTATTCATCCATCTGCTGATCAAATGGATAAACCTTCTGTTTTAGAACTATCCAAGGACATGGTATCTACAAATGGTTGCATTGGCCTTTATTTCATTCTACCCTATCCAATAGGGAGTATCAATCATAACTTGCTTAACTTCAATATTTCGCAGTCGATTGTTCTGTGCATGACAGTTAGTGATGCCATTAGTAATTCAACTTTAAGTCTCATATATTACTCTGTGGCATAAATATGACCTACACTGATGAATTGTATGGGCTAATATTTGTTGTAATCCTTCTAGAAACATCATTAACAAGTAATATGATTGCTTACCTAGTTCAAGGTTCATTGTCGAACACATTTGTCTCTTCCTTGTTCCTTCTTGCTCCAAACTAAAGATTTCTTGTTTCTCTTCACTATTTTTGTTTTCTTAGGTCAAAGATCAAACATATTTTCTGTCGCATCTCTCACAGAACCAGCTTAAGAGGCTTTTATTCCCTCTTGGTTGTATTCCGAAGGTTTGTTCAAGCGTACTTTACAGCATTTATTATCGTGGGTTTTTAAGATATCATGAATCATATAAATGCTTCAATTAGTAAGTTCATATCATGAATTGGCTCTTGCTCCTATCTCAACTCTGGCTACTGTCTTTCATCTAGTGCATACTTGTGTGCTTGTGATCTTGTTAATATGTTTTCCTTGAGTTGTAACTTCAGCAATATTTTTCCCTCTCAGCCTTATATTCCCTCAGCAATTATCTGCAGGATGAGGTTCGCAAACTTGCTGCAAAATTCAACTTACCAAACAAAGACAGAAAGGATTCACAAGGAATATGTTTCCTTGGGAAGGTAGTTTCGGATGTGATGATTTATGCATCTATTTTACTTGGAGGACTGAATGCTTTCTCAAAATTCATTCGTCAATGCCAATATATCTAATATTTTAGTGTTGCCATTTTATATTTTTGAAAAATATCCTTCTACATGTTATCACTATTTAAAATGACAGAATAGCTCAAAGAATTTAGTATAAAATTTGCTAACTGTTGAAGAATTTTTCTGATGGCTAAACTTTCCCCGAGCTGGGTTTTTATTTCCTTATTCTTCATGACTGAAAAAAATCTTGTTCATCCAGTTTCAGAAGAAGGGTCTCTGTGTAAGGTATCTTGCGGTAGAATTAATGCAGAATCTGTTTCTTTGATGTGTAGATAAAATTTAGTGAATTTGTGGCGAGACATATTGGGGAGGACGAAGGTGTGATATTGGAGGCAGAGTCGGGAGATTTTCTTGGCAAACATCGAGGGTTTTGGTTTTACACGATTGGCCAACGCCAAGGCCTAAAACTTCCTGGAGGACCTTGGTATGCCTTTTTAGTTTATTTTCAAGAATAAGTTCGATGTTTCCAGCTGTTTAGCGGTTTATGACCACTTCCAAATCTTCACGAACATCTTAAATTCTACTTGGTAGAGTAGATTTGAACGTTTCAGGGTGCCAGTTTAGTTTTAAATAACTAATCACGTTAGATTATCTCCAATTTGTTGCTTCTCAAGTTAATATTCTTTTGTTATGTCTCTTCAGCTTTTATGCATTGGTCTAATGCAGGTATGTTGTGGAGAAAGATATTAGGAACAATGTAGTATTTGTGTCGAGAAATTATTTCTCACTTGACAAAAGAAGGCGGTTGTTTCGAGTTGGATCGTTGAAATGGCTTAGTGGGTTGGTTCCAGACCAATTTACTGAGCTTCAATGTAAGGTAAAGTTTCAGCTTCCATCTCTCATATTTCTCGCTAGTTTCACTACTTCAAGTTGGCCTGCCTGTAACTGTTCAACAGGAGTCAGTTTATTCGAGCAAATTTGTGTTTGAACTGATGTCATTTCCAGTTCCCCCTTATTTGATGATTTTATACAATTTTTAGGTCCGACATGGCCCGAGTTTCTATAGTTGCAATTTGACAATGGAACGCAATGAAGATAGTGATGAAGATGTTGCAGTTGTCCATCTAAGCGAAGATGACCAAGGCCTTGCAGCAGGCCAGTTTGCAGCCTTTTATGAGGGAAGGAGATGCATTGGGTCGGGTGTGATTTTAGAATCTTGGGATGATCAGGGGTTTCCTGTATGTGCAAAGGCTCTTGAAATTGCACAAATGGAAGATAAGTCGAAGCTTGGTAAACCAATAAAGATAAAAGTTAAACCAGAGAATGCTACGAAAGAATGTGAACCTAAAGATGATACAAAAATACACGGCGTGTTAAAGAATCAACAAACCACTGTGGTTGAGCAAACCGGAGAAGTGGCTTCGGATGAAGCACTCACCACAGCCCCAATGAAATGGCTTGGAAGCCTTCGGAAGAAGCTGCTGCAGGTATTTTAATTTTGCCTTAGTTTAGAAATCGAATCCTTGACTACAGCCCTGCAATGAGAGTGAAACCCATATTTTATTCCTTTGAATCTGTTGACTGAATTATGACATATTTTTATGTTTGTGATGGAGCAAATATAGGAGCTCTCCTCTCTTATTCCGACGCCTTTTGAATCGACTGAGTTGTTTGTTGTTTCTTTTAACTGAGGGATCAAATCGATTTAATCATGTCT

mRNA sequence

TTTATTCGGCTCGTCTTCCTTTCGGAGCAAGAAGAATGCCGCGGCGGTGGATATAAGGAGGAATCAATTACAGTGATGCTGAGACTGAGAGTGAAGCCCGTTTGGTGCACTCTTCCGATCTCCCATTTCACTTCTCTTCCCCTTCGCCCCTCAATTCTCAGACCTGTTCTCCGCCGCCACAGGCCTCGACTCTTCCTCACCCGATTCTCCGCCTCCTCTGCTGTGCATAAAGCCTCGGAATCTTATTCAGCTAAAGGCCTCTCGGATGGCGACTTGGAGCCTTTCCTCTCATGTTCGATGCCTCGCTATCCGCTCAAAGTTGCTGTTCTTCTTAGCGGTGGCGTCGATAGCAGCGTCGCCCTCCGCCTCCTCCACGCTGCCGGCCACTCCTGCACTGCCTTCTATCTCAAGATATGGTTTCAGTTTATTCGGCTCGTCTTCCTTTCGGAGCAAGAAGAATGCCGCGGCGGTGGATATAAGGAGGAATCAATTACAGTGATGCTGAGACTGAGAGTGAAGCCCGTTTGGTGCACTCTTCCGATCTCCCATTTCACTTCTCTTCCCCTTCGCCCCTCAATTCTCAGACCTGTTCTCCGCCGCCACAGGCCTCGACTCTTCCTCACCCGATTCTCCGCCTCCTCTGCTGTGCATAAAGCCTCGGAATCTTATTCAGCTAAAGGCCTCTCGGATGGCGACTTGGAGCCTTTCCTCTCATGTTCGATGCCTCGCTATCCGCTCAAAGTTGCTGTTCTTCTTAGCGGTGGCGTCGATAGCAGCGTCGCCCTCCGCCTCCTCCACGCTGCCGGCCACTCCTGCACTGCCTTCTATCTCAAGATATGGTTTCAGGAAGACTTCGAGAATTTTTGGTCAGAATGCCCATGGGACGATGATTTGAAGTATGCTGAAGCTGTTTGTGATCAGGTTGATGTACCATTGGAAGTGGTTCATTTGACAGAGGAATATTGGAGGAACGTGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGTACTCCAAACCCTGATGTCCTTTGTAACACAAGAATAAAGTTTGGTGCATTCATGGATGCAATAAGCAATATGGAGTTTGATTATGTTGCTTCAGGGCATTATGCGAATGTTATTCATCCATCTGCTGATCAAATGGATAAACCTTCTGTTTTAGAACTATCCAAGGACATGTCGATTGTTCTGTGCATGACAGTCAAAGATCAAACATATTTTCTGTCGCATCTCTCACAGAACCAGCTTAAGAGGCTTTTATTCCCTCTTGGTTGTATTCCGAAGGATGAGGTTCGCAAACTTGCTGCAAAATTCAACTTACCAAACAAAGACAGAAAGGATTCACAAGGAATATGTTTCCTTGGGAAGATAAAATTTAGTGAATTTGTGGCGAGACATATTGGGGAGGACGAAGGTGTGATATTGGAGGCAGAGTCGGGAGATTTTCTTGGCAAACATCGAGGGTTTTGGTTTTACACGATTGGCCAACGCCAAGGCCTAAAACTTCCTGGAGGACCTTGGTATGTTGTGGAGAAAGATATTAGGAACAATGTAGTATTTGTGTCGAGAAATTATTTCTCACTTGACAAAAGAAGGCGGTTGTTTCGAGTTGGATCGTTGAAATGGCTTAGTGGGTTGGTTCCAGACCAATTTACTGAGCTTCAATGTAAGGTCCGACATGGCCCGAGTTTCTATAGTTGCAATTTGACAATGGAACGCAATGAAGATAGTGATGAAGATGTTGCAGTTGTCCATCTAAGCGAAGATGACCAAGGCCTTGCAGCAGGCCAGTTTGCAGCCTTTTATGAGGGAAGGAGATGCATTGGGTCGGGTGTGATTTTAGAATCTTGGGATGATCAGGGGTTTCCTGTATGTGCAAAGGCTCTTGAAATTGCACAAATGGAAGATAAGTCGAAGCTTGGTAAACCAATAAAGATAAAAGTTAAACCAGAGAATGCTACGAAAGAATGTGAACCTAAAGATGATACAAAAATACACGGCGTGTTAAAGAATCAACAAACCACTGTGGTTGAGCAAACCGGAGAAGTGGCTTCGGATGAAGCACTCACCACAGCCCCAATGAAATGGCTTGGAAGCCTTCGGAAGAAGCTGCTGCAGGTATTTTAATTTTGCCTTAGTTTAGAAATCGAATCCTTGACTACAGCCCTGCAATGAGAGTGAAACCCATATTTTATTCCTTTGAATCTGTTGACTGAATTATGACATATTTTTATGTTTGTGATGGAGCAAATATAGGAGCTCTCCTCTCTTATTCCGACGCCTTTTGAATCGACTGAGTTGTTTGTTGTTTCTTTTAACTGAGGGATCAAATCGATTTAATCATGTCT

Coding sequence (CDS)

TTTATTCGGCTCGTCTTCCTTTCGGAGCAAGAAGAATGCCGCGGCGGTGGATATAAGGAGGAATCAATTACAGTGATGCTGAGACTGAGAGTGAAGCCCGTTTGGTGCACTCTTCCGATCTCCCATTTCACTTCTCTTCCCCTTCGCCCCTCAATTCTCAGACCTGTTCTCCGCCGCCACAGGCCTCGACTCTTCCTCACCCGATTCTCCGCCTCCTCTGCTGTGCATAAAGCCTCGGAATCTTATTCAGCTAAAGGCCTCTCGGATGGCGACTTGGAGCCTTTCCTCTCATGTTCGATGCCTCGCTATCCGCTCAAAGTTGCTGTTCTTCTTAGCGGTGGCGTCGATAGCAGCGTCGCCCTCCGCCTCCTCCACGCTGCCGGCCACTCCTGCACTGCCTTCTATCTCAAGATATGGTTTCAGTTTATTCGGCTCGTCTTCCTTTCGGAGCAAGAAGAATGCCGCGGCGGTGGATATAAGGAGGAATCAATTACAGTGATGCTGAGACTGAGAGTGAAGCCCGTTTGGTGCACTCTTCCGATCTCCCATTTCACTTCTCTTCCCCTTCGCCCCTCAATTCTCAGACCTGTTCTCCGCCGCCACAGGCCTCGACTCTTCCTCACCCGATTCTCCGCCTCCTCTGCTGTGCATAAAGCCTCGGAATCTTATTCAGCTAAAGGCCTCTCGGATGGCGACTTGGAGCCTTTCCTCTCATGTTCGATGCCTCGCTATCCGCTCAAAGTTGCTGTTCTTCTTAGCGGTGGCGTCGATAGCAGCGTCGCCCTCCGCCTCCTCCACGCTGCCGGCCACTCCTGCACTGCCTTCTATCTCAAGATATGGTTTCAGGAAGACTTCGAGAATTTTTGGTCAGAATGCCCATGGGACGATGATTTGAAGTATGCTGAAGCTGTTTGTGATCAGGTTGATGTACCATTGGAAGTGGTTCATTTGACAGAGGAATATTGGAGGAACGTGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGTACTCCAAACCCTGATGTCCTTTGTAACACAAGAATAAAGTTTGGTGCATTCATGGATGCAATAAGCAATATGGAGTTTGATTATGTTGCTTCAGGGCATTATGCGAATGTTATTCATCCATCTGCTGATCAAATGGATAAACCTTCTGTTTTAGAACTATCCAAGGACATGTCGATTGTTCTGTGCATGACAGTCAAAGATCAAACATATTTTCTGTCGCATCTCTCACAGAACCAGCTTAAGAGGCTTTTATTCCCTCTTGGTTGTATTCCGAAGGATGAGGTTCGCAAACTTGCTGCAAAATTCAACTTACCAAACAAAGACAGAAAGGATTCACAAGGAATATGTTTCCTTGGGAAGATAAAATTTAGTGAATTTGTGGCGAGACATATTGGGGAGGACGAAGGTGTGATATTGGAGGCAGAGTCGGGAGATTTTCTTGGCAAACATCGAGGGTTTTGGTTTTACACGATTGGCCAACGCCAAGGCCTAAAACTTCCTGGAGGACCTTGGTATGTTGTGGAGAAAGATATTAGGAACAATGTAGTATTTGTGTCGAGAAATTATTTCTCACTTGACAAAAGAAGGCGGTTGTTTCGAGTTGGATCGTTGAAATGGCTTAGTGGGTTGGTTCCAGACCAATTTACTGAGCTTCAATGTAAGGTCCGACATGGCCCGAGTTTCTATAGTTGCAATTTGACAATGGAACGCAATGAAGATAGTGATGAAGATGTTGCAGTTGTCCATCTAAGCGAAGATGACCAAGGCCTTGCAGCAGGCCAGTTTGCAGCCTTTTATGAGGGAAGGAGATGCATTGGGTCGGGTGTGATTTTAGAATCTTGGGATGATCAGGGGTTTCCTGTATGTGCAAAGGCTCTTGAAATTGCACAAATGGAAGATAAGTCGAAGCTTGGTAAACCAATAAAGATAAAAGTTAAACCAGAGAATGCTACGAAAGAATGTGAACCTAAAGATGATACAAAAATACACGGCGTGTTAAAGAATCAACAAACCACTGTGGTTGAGCAAACCGGAGAAGTGGCTTCGGATGAAGCACTCACCACAGCCCCAATGAAATGGCTTGGAAGCCTTCGGAAGAAGCTGCTGCAGGTATTTTAA

Protein sequence

FIRLVFLSEQEECRGGGYKEESITVMLRLRVKPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESYSAKGLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQFIRLVFLSEQEECRGGGYKEESITVMLRLRVKPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESYSAKGLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAEAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMSIVLCMTVKDQTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPENATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF
Homology
[Close]
BLAST of Cp4.1LG16g08850 vs. ExPASy Swiss-Prot
Match: O51625 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680) OX=224326 GN=mnmA PE=3 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 6.6e-99
Identity = 170/372 (45.70%), Postives = 249/372 (66.94%), Query Frame = 0 

Query: 246 LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAEAV 305
           +K+AVLLSGGVDSSVAL R+++    +   +YLKIW +++  ++   CPW +DL Y EA+
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDEL-SYIGNCPWQEDLNYVEAI 60

Query: 306 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 365
           C++ +VP E+++  +EY+  VVSY IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NSQYD 120

Query: 366 YVASGHYANVIHPSADQMDKPSVLELSKDMSIVLCMTVKDQTYFLSHLSQNQLKRLLFPL 425
            V +GHYA +        +   +L+ +KD        +KDQ+YFLSHLSQ Q+ +L FPL
Sbjct: 121 LVVTGHYAKI-----QIKESKFLLKQAKD-------KIKDQSYFLSHLSQKQMSKLYFPL 180

Query: 426 GCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFL 485
           G + K EVR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +
Sbjct: 181 GTLLKSEVRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKII 240

Query: 486 GKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRLFRVGSLKWL 545
           G H G+WF+T+GQR+G+KL  GPW+V+EKD+  N++++S N   L + +R F V  + W+
Sbjct: 241 GIHNGYWFFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWI 300

Query: 546 SGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDDQGLAAGQFAAFYE 605
           +   P  F   + K+RHG   YSC L +  N     ++  + L++ DQG++ GQFA FY+
Sbjct: 301 ND-TPTNFENFKIKIRHGEKKYSCKLKLITN-----NLMEISLNKKDQGISPGQFAIFYK 352

Query: 606 GRRCIGSGVILE 617
              C+G   I +
Sbjct: 361 NTECLGGAKIFK 352

[Close]
BLAST of Cp4.1LG16g08850 vs. ExPASy Swiss-Prot
Match: B7J2N7 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) OX=445985 GN=mnmA PE=3 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 6.6e-99
Identity = 170/372 (45.70%), Postives = 249/372 (66.94%), Query Frame = 0 

Query: 246 LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAEAV 305
           +K+AVLLSGGVDSSVAL R+++    +   +YLKIW +++  ++   CPW +DL Y EA+
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDEL-SYIGNCPWQEDLNYVEAI 60

Query: 306 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 365
           C++ +VP E+++  +EY+  VVSY IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NSQYD 120

Query: 366 YVASGHYANVIHPSADQMDKPSVLELSKDMSIVLCMTVKDQTYFLSHLSQNQLKRLLFPL 425
            V +GHYA +        +   +L+ +KD        +KDQ+YFLSHLSQ Q+ +L FPL
Sbjct: 121 LVVTGHYAKI-----QIKESKFLLKQAKD-------KIKDQSYFLSHLSQKQISKLYFPL 180

Query: 426 GCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFL 485
           G + K EVR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +
Sbjct: 181 GTLLKSEVRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKII 240

Query: 486 GKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRLFRVGSLKWL 545
           G H G+WF+T+GQR+G+KL  GPW+V+EKD+  N++++S N   L + +R F V  + W+
Sbjct: 241 GIHNGYWFFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWI 300

Query: 546 SGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDDQGLAAGQFAAFYE 605
           +   P  F   + K+RHG   YSC L +  N     ++  + L++ DQG++ GQFA FY+
Sbjct: 301 ND-TPTNFENFKIKIRHGEKKYSCKLKLITN-----NLMEISLNKKDQGISPGQFAIFYK 352

Query: 606 GRRCIGSGVILE 617
              C+G   I +
Sbjct: 361 NTECLGGAKIFK 352

[Close]
BLAST of Cp4.1LG16g08850 vs. ExPASy Swiss-Prot
Match: Q0SMH1 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia afzelii (strain PKo) OX=390236 GN=mnmA PE=3 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 3.3e-98
Identity = 170/372 (45.70%), Postives = 247/372 (66.40%), Query Frame = 0 

Query: 246 LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAEAV 305
           +K+AVLLSGGVDSSVAL R+++    S   +YLKIW +++  ++   CPW +DL Y EAV
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYTSIKCYYLKIWLEDEL-SYIGSCPWQEDLNYVEAV 60

Query: 306 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 365
           C + +VP E+++  +EY+  VVSY I+E + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CSKFNVPYEIINFQKEYYNKVVSYTIKELKNGNTPSPDIFCNQRIKFGAFFEKI-NEQYD 120

Query: 366 YVASGHYANVIHPSADQMDKPSVLELSKDMSIVLCMTVKDQTYFLSHLSQNQLKRLLFPL 425
            V +GHYA +        DK   L+ +KD        +KDQ+YFLSHLSQ Q+ +L FPL
Sbjct: 121 LVVTGHYAKI-----QTKDKKFFLKQAKD-------KIKDQSYFLSHLSQKQMSKLYFPL 180

Query: 426 GCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFL 485
           G + K EVR++A   NLPNK+RKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +
Sbjct: 181 GTLLKSEVRQIAKNINLPNKNRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKII 240

Query: 486 GKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRLFRVGSLKWL 545
           G H G+WF+T+GQR+G+KL  GPW+V+EKD+  N++++S N     + +R F V  + W+
Sbjct: 241 GTHNGYWFFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYSKQAKRKFLVHEIHWI 300

Query: 546 SGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDDQGLAAGQFAAFYE 605
           +G  P  F   + K+RHG   YSC L +       +++  + L++ D G++ GQFA FY+
Sbjct: 301 NG-TPSDFENFKIKIRHGEKKYSCKLKL-----IAKNLIEISLNKKDYGISPGQFAIFYK 352

Query: 606 GRRCIGSGVILE 617
              C+G   I +
Sbjct: 361 NTECLGGAKIFK 352

[Close]
BLAST of Cp4.1LG16g08850 vs. ExPASy Swiss-Prot
Match: Q660I8 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia bavariensis (strain ATCC BAA-2496 / DSM 23469 / PBi) OX=290434 GN=mnmA PE=3 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 4.3e-98
Identity = 168/372 (45.16%), Postives = 249/372 (66.94%), Query Frame = 0 

Query: 246 LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAEAV 305
           +K+AVLLSGGVDSS+AL ++++    +   +YLKIW +++  ++   CPW +DL Y EAV
Sbjct: 1   MKIAVLLSGGVDSSIALYKVINKGYTNIKCYYLKIWLEDEL-SYIGNCPWQEDLNYVEAV 60

Query: 306 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 365
           C++ ++P E+++  +EY+  VV Y IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNIPYEIINFQKEYYNKVVIYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NEQYD 120

Query: 366 YVASGHYANVIHPSADQMDKPSVLELSKDMSIVLCMTVKDQTYFLSHLSQNQLKRLLFPL 425
            V +GHYA +        +K   L+ +KD        +KDQ+YFLSHLSQ Q+ +L FPL
Sbjct: 121 LVVTGHYARI-----QIKEKKFFLKQAKD-------KIKDQSYFLSHLSQEQMSKLYFPL 180

Query: 426 GCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFL 485
           G + K EVR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +
Sbjct: 181 GTLFKSEVRQIAKSINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIVEKETGKII 240

Query: 486 GKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRLFRVGSLKWL 545
           G H G+WF+T+GQR+G+KL  GPW+V+EKD+  N++++S N   L + +R F V  + W+
Sbjct: 241 GIHNGYWFFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWI 300

Query: 546 SGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDDQGLAAGQFAAFYE 605
           +G+ P  F   + K+RHG   YSC L +  N     ++  + L++ D G++ GQFA FY+
Sbjct: 301 NGM-PSDFENFKIKIRHGEKKYSCKLRLITN-----NLIEIFLNKKDHGISPGQFAIFYK 352

Query: 606 GRRCIGSGVILE 617
              C+G   I +
Sbjct: 361 NTECLGGAKIFK 352

[Close]
BLAST of Cp4.1LG16g08850 vs. ExPASy Swiss-Prot
Match: B2S125 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) OX=314723 GN=mnmA PE=3 SV=2)

HSP 1 Score: 354.4 bits (908), Expect = 3.0e-96
Identity = 170/373 (45.58%), Postives = 247/373 (66.22%), Query Frame = 0 

Query: 246 LKVAVLLSGGVDSSVALRLLHAAGH-SCTAFYLKIWFQEDFENFWSECPWDDDLKYAEAV 305
           +K+AVLLSGGVDSSVAL  +   G+ +   +YLKIW +++  ++  ECPW++D+ Y EAV
Sbjct: 1   MKIAVLLSGGVDSSVALYTMIQKGYKNIKCYYLKIWLEDEL-SYIGECPWEEDINYVEAV 60

Query: 306 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 365
           C + +VP E++ L +EY++ VV+Y IEE + G TP+PD+ CN RIKFGAF D I N  +D
Sbjct: 61  CTKFNVPYEIISLQDEYYKRVVTYAIEELKIGNTPSPDIFCNQRIKFGAFFDKI-NEHYD 120

Query: 366 YVASGHYANVIHPSADQMDKPSVLELSKDMSIVLCMTVKDQTYFLSHLSQNQLKRLLFPL 425
            + +GHYA +     +  +   +L+ +KD        +KDQ+YFLSHLS+ Q+ +L FPL
Sbjct: 121 LIVTGHYAKI-----ENKNNHYILKQAKD-------KIKDQSYFLSHLSREQISKLHFPL 180

Query: 426 GCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFL 485
           G + K E+R++A K +LPNK+RKDSQGICFLGKIK+ EF+  H+GE +G I+E E+G  L
Sbjct: 181 GDLLKTEIRQIAHKIDLPNKNRKDSQGICFLGKIKYDEFIKYHLGELKGNIIEQETGKIL 240

Query: 486 GKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRLFRVGSLKWL 545
           G H G+WF+TIGQR+G+KL  GPW+V EKDI+NN++++S +   L + +  F V    W+
Sbjct: 241 GTHNGYWFFTIGQRKGIKLSHGPWFVTEKDIQNNIIYISNSVNYLAQGKHQFLVHKTNWI 300

Query: 546 SGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDDQGLAAGQFAAFYE 605
           +   P    +L  K+RHG     C + M +N     D+  V L E D G++ GQF  FY+
Sbjct: 301 N--KPVNNIDLSAKIRHGEKKIKCKIEMLKN-----DIIKVDLEEKDYGISPGQFCIFYQ 352

Query: 606 GRRCIGSGVILES 618
              C+G   IL++
Sbjct: 361 EDECLGGAQILQT 352

[Close]
BLAST of Cp4.1LG16g08850 vs. NCBI nr
Match: XP_023513286.1 (uncharacterized protein LOC111777800 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1090 bits (2819), Expect = 0.0
Identity = 536/543 (98.71%), Postives = 536/543 (98.71%), Query Frame = 0 

Query: 167 MLRLRVKPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESYSAK 226
           MLRLRVKPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESYSAK
Sbjct: 1   MLRLRVKPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESYSAK 60

Query: 227 GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 286
           GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE
Sbjct: 61  GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 120

Query: 287 NFWSECPWDDDLKYAEAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 346
           NFWSECPWDDDLKYAEAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN
Sbjct: 121 NFWSECPWDDDLKYAEAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 180

Query: 347 TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMSIVLCMTVKDQT 406
           TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDM       VKDQT
Sbjct: 181 TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDM-------VKDQT 240

Query: 407 YFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVAR 466
           YFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVAR
Sbjct: 241 YFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVAR 300

Query: 467 HIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNY 526
           HIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNY
Sbjct: 301 HIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNY 360

Query: 527 FSLDKRRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVH 586
           FSLDKRRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVH
Sbjct: 361 FSLDKRRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVH 420

Query: 587 LSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIK 646
           LSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIK
Sbjct: 421 LSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIK 480

Query: 647 IKVKPENATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLL 706
           IKVKPENATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLL
Sbjct: 481 IKVKPENATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLL 536

Query: 707 QVF 709
           QVF
Sbjct: 541 QVF 536

[Close]
BLAST of Cp4.1LG16g08850 vs. NCBI nr
Match: XP_022944568.1 (uncharacterized protein LOC111448983 [Cucurbita moschata])

HSP 1 Score: 1080 bits (2792), Expect = 0.0
Identity = 531/543 (97.79%), Postives = 534/543 (98.34%), Query Frame = 0 

Query: 167 MLRLRVKPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESYSAK 226
           MLRLRV PVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASES SAK
Sbjct: 1   MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSAK 60

Query: 227 GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 286
           GLSDGDLEPFLSCSMPR+PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE
Sbjct: 61  GLSDGDLEPFLSCSMPRHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 120

Query: 287 NFWSECPWDDDLKYAEAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 346
           NFWSECPWDDDLKYA+AVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN
Sbjct: 121 NFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 180

Query: 347 TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMSIVLCMTVKDQT 406
           TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQM+KPSVLELSKDM       VKDQT
Sbjct: 181 TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMEKPSVLELSKDM-------VKDQT 240

Query: 407 YFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVAR 466
           YFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVAR
Sbjct: 241 YFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVAR 300

Query: 467 HIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNY 526
           HIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNY
Sbjct: 301 HIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNY 360

Query: 527 FSLDKRRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVH 586
           FSLDKRRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVH
Sbjct: 361 FSLDKRRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVH 420

Query: 587 LSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIK 646
           LSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIK
Sbjct: 421 LSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIK 480

Query: 647 IKVKPENATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLL 706
           IKVKPENATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLL
Sbjct: 481 IKVKPENATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLL 536

Query: 707 QVF 709
           QVF
Sbjct: 541 QVF 536

[Close]
BLAST of Cp4.1LG16g08850 vs. NCBI nr
Match: KAG6570391.1 (hypothetical protein SDJN03_29306, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1075 bits (2781), Expect = 0.0
Identity = 529/543 (97.42%), Postives = 533/543 (98.16%), Query Frame = 0 

Query: 167 MLRLRVKPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESYSAK 226
           MLRLRV PVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASES SAK
Sbjct: 1   MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSAK 60

Query: 227 GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 286
           GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE
Sbjct: 61  GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 120

Query: 287 NFWSECPWDDDLKYAEAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 346
           NFWSECPWDDDLKYA+AVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN
Sbjct: 121 NFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 180

Query: 347 TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMSIVLCMTVKDQT 406
           TRIKFGAFMDAISNMEF+YVASGHYANVIHPSADQM+KPSVLELSKDM       VKDQT
Sbjct: 181 TRIKFGAFMDAISNMEFNYVASGHYANVIHPSADQMEKPSVLELSKDM-------VKDQT 240

Query: 407 YFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVAR 466
           YFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVAR
Sbjct: 241 YFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVAR 300

Query: 467 HIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNY 526
           HIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNY
Sbjct: 301 HIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNY 360

Query: 527 FSLDKRRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVH 586
           FSLDKRRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNE SDEDVAVVH
Sbjct: 361 FSLDKRRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVH 420

Query: 587 LSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIK 646
           LSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIK
Sbjct: 421 LSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIK 480

Query: 647 IKVKPENATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLL 706
           IKVKPENA+KECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLL
Sbjct: 481 IKVKPENASKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLL 536

Query: 707 QVF 709
           QVF
Sbjct: 541 QVF 536

[Close]
BLAST of Cp4.1LG16g08850 vs. NCBI nr
Match: KAG7010265.1 (mnmA [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1072 bits (2773), Expect = 0.0
Identity = 532/582 (91.41%), Postives = 539/582 (92.61%), Query Frame = 0 

Query: 167 MLRLRVKPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESYSAK 226
           MLRLRV PVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASES SAK
Sbjct: 1   MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSAK 60

Query: 227 GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 286
           GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE
Sbjct: 61  GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 120

Query: 287 NFWSECPWDDDLKYAEAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 346
           NFWSECPWDDDLKYA+AVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN
Sbjct: 121 NFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 180

Query: 347 TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDM------------ 406
           TRIKFGAFMDAISNMEF+YVASGHYANVIHPSADQM+KPSVLELSKDM            
Sbjct: 181 TRIKFGAFMDAISNMEFNYVASGHYANVIHPSADQMEKPSVLELSKDMVSTNGCIGLYFI 240

Query: 407 ---------------------------SIVLCMTVKDQTYFLSHLSQNQLKRLLFPLGCI 466
                                      + +LCMTVKDQTYFLSHLSQNQLKRLLFPLGCI
Sbjct: 241 LPYPIGSINHNLLNFNISQSIVFVLTFAYILCMTVKDQTYFLSHLSQNQLKRLLFPLGCI 300

Query: 467 PKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKH 526
           PKDEVRKLAAKFNLPNKDRKDSQGICFLGK+KFSEFVARHIGEDEGVILEAESGDFLGKH
Sbjct: 301 PKDEVRKLAAKFNLPNKDRKDSQGICFLGKVKFSEFVARHIGEDEGVILEAESGDFLGKH 360

Query: 527 RGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRLFRVGSLKWLSGL 586
           RGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRLFRVGSLKWLSGL
Sbjct: 361 RGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRLFRVGSLKWLSGL 420

Query: 587 VPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDDQGLAAGQFAAFYEGRR 646
           VPDQFTELQCKVRHGPSFYSCNLTMERNE SDEDVAVVHLSEDDQGLAAGQFAAFYEGRR
Sbjct: 421 VPDQFTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDDQGLAAGQFAAFYEGRR 480

Query: 647 CIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPENATKECEPKDDTKIH 706
           CIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPENA+KECEPKDDTKIH
Sbjct: 481 CIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPENASKECEPKDDTKIH 540

Query: 707 GVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 709
           GVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF
Sbjct: 541 GVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 582

[Close]
BLAST of Cp4.1LG16g08850 vs. NCBI nr
Match: XP_022985745.1 (uncharacterized protein LOC111483706 [Cucurbita maxima])

HSP 1 Score: 1061 bits (2743), Expect = 0.0
Identity = 524/543 (96.50%), Postives = 529/543 (97.42%), Query Frame = 0 

Query: 167 MLRLRVKPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESYSAK 226
           MLRLRVKPVWCTLPISHFTSL LRPSILRPVLRRHRPRLF+TRFSASSAVHKASES SAK
Sbjct: 1   MLRLRVKPVWCTLPISHFTSLTLRPSILRPVLRRHRPRLFITRFSASSAVHKASESSSAK 60

Query: 227 GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 286
           GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE
Sbjct: 61  GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 120

Query: 287 NFWSECPWDDDLKYAEAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 346
           NFWSECPWDDDLKYA+AVCD+VDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN
Sbjct: 121 NFWSECPWDDDLKYAKAVCDRVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 180

Query: 347 TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMSIVLCMTVKDQT 406
           TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDM       VKDQT
Sbjct: 181 TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDM-------VKDQT 240

Query: 407 YFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVAR 466
           YFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVAR
Sbjct: 241 YFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVAR 300

Query: 467 HIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNY 526
           HIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNY
Sbjct: 301 HIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNY 360

Query: 527 FSLDKRRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVH 586
           FSLDKRRR FRVGSLKWLSGLVPDQFT LQCKVRHGPSFYSC LTMER+EDSDEDVAVV 
Sbjct: 361 FSLDKRRRSFRVGSLKWLSGLVPDQFTGLQCKVRHGPSFYSCILTMERDEDSDEDVAVVL 420

Query: 587 LSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIK 646
           LSEDDQGLAAGQFAAFY+GRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIK
Sbjct: 421 LSEDDQGLAAGQFAAFYQGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIK 480

Query: 647 IKVKPENATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLL 706
           IKVKPENATKECE KDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLL
Sbjct: 481 IKVKPENATKECETKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLL 536

Query: 707 QVF 709
           QVF
Sbjct: 541 QVF 536

[Close]
BLAST of Cp4.1LG16g08850 vs. ExPASy TrEMBL
Match: A0A6J1FY63 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita moschata OX=3662 GN=LOC111448983 PE=3 SV=1)

HSP 1 Score: 1080 bits (2792), Expect = 0.0
Identity = 531/543 (97.79%), Postives = 534/543 (98.34%), Query Frame = 0 

Query: 167 MLRLRVKPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESYSAK 226
           MLRLRV PVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASES SAK
Sbjct: 1   MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSAK 60

Query: 227 GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 286
           GLSDGDLEPFLSCSMPR+PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE
Sbjct: 61  GLSDGDLEPFLSCSMPRHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 120

Query: 287 NFWSECPWDDDLKYAEAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 346
           NFWSECPWDDDLKYA+AVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN
Sbjct: 121 NFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 180

Query: 347 TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMSIVLCMTVKDQT 406
           TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQM+KPSVLELSKDM       VKDQT
Sbjct: 181 TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMEKPSVLELSKDM-------VKDQT 240

Query: 407 YFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVAR 466
           YFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVAR
Sbjct: 241 YFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVAR 300

Query: 467 HIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNY 526
           HIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNY
Sbjct: 301 HIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNY 360

Query: 527 FSLDKRRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVH 586
           FSLDKRRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVH
Sbjct: 361 FSLDKRRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVH 420

Query: 587 LSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIK 646
           LSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIK
Sbjct: 421 LSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIK 480

Query: 647 IKVKPENATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLL 706
           IKVKPENATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLL
Sbjct: 481 IKVKPENATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLL 536

Query: 707 QVF 709
           QVF
Sbjct: 541 QVF 536

[Close]
BLAST of Cp4.1LG16g08850 vs. ExPASy TrEMBL
Match: A0A6J1JC61 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita maxima OX=3661 GN=LOC111483706 PE=3 SV=1)

HSP 1 Score: 1061 bits (2743), Expect = 0.0
Identity = 524/543 (96.50%), Postives = 529/543 (97.42%), Query Frame = 0 

Query: 167 MLRLRVKPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESYSAK 226
           MLRLRVKPVWCTLPISHFTSL LRPSILRPVLRRHRPRLF+TRFSASSAVHKASES SAK
Sbjct: 1   MLRLRVKPVWCTLPISHFTSLTLRPSILRPVLRRHRPRLFITRFSASSAVHKASESSSAK 60

Query: 227 GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 286
           GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE
Sbjct: 61  GLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 120

Query: 287 NFWSECPWDDDLKYAEAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 346
           NFWSECPWDDDLKYA+AVCD+VDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN
Sbjct: 121 NFWSECPWDDDLKYAKAVCDRVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 180

Query: 347 TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMSIVLCMTVKDQT 406
           TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDM       VKDQT
Sbjct: 181 TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDM-------VKDQT 240

Query: 407 YFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVAR 466
           YFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVAR
Sbjct: 241 YFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVAR 300

Query: 467 HIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNY 526
           HIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNY
Sbjct: 301 HIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNY 360

Query: 527 FSLDKRRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVH 586
           FSLDKRRR FRVGSLKWLSGLVPDQFT LQCKVRHGPSFYSC LTMER+EDSDEDVAVV 
Sbjct: 361 FSLDKRRRSFRVGSLKWLSGLVPDQFTGLQCKVRHGPSFYSCILTMERDEDSDEDVAVVL 420

Query: 587 LSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIK 646
           LSEDDQGLAAGQFAAFY+GRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIK
Sbjct: 421 LSEDDQGLAAGQFAAFYQGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIK 480

Query: 647 IKVKPENATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLL 706
           IKVKPENATKECE KDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLL
Sbjct: 481 IKVKPENATKECETKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLL 536

Query: 707 QVF 709
           QVF
Sbjct: 541 QVF 536

[Close]
BLAST of Cp4.1LG16g08850 vs. ExPASy TrEMBL
Match: A0A0A0KX16 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis sativus OX=3659 GN=Csa_4G291390 PE=3 SV=1)

HSP 1 Score: 981 bits (2535), Expect = 0.0
Identity = 481/545 (88.26%), Postives = 503/545 (92.29%), Query Frame = 0 

Query: 166 VMLRLRVKP-VWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESYS 225
           +MLRLRV P VWC++PISHFTSLPL PSILRP+LRRH PRLFLTRFSASSAVH  S S S
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSS 60

Query: 226 AKGLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 285
           +K LSD D  PFLSCSMP YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 61  SKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120

Query: 286 FENFWSECPWDDDLKYAEAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 345
           FENFWSECPWDDDLKYA+AVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180

Query: 346 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMSIVLCMTVKD 405
           CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDM       VKD
Sbjct: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDM-------VKD 240

Query: 406 QTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFV 465
           QTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFV
Sbjct: 241 QTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFV 300

Query: 466 ARHIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSR 525
           ARHIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGL+LPGGPWYVVEKDI+NNVVFVSR
Sbjct: 301 ARHIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSR 360

Query: 526 NYFSLDKRRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAV 585
           NYFS DKRRR+FRVGSLKWLSG  PDQFTELQCKVRHGPSFYSCNLTME +ED  EDVAV
Sbjct: 361 NYFSFDKRRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAV 420

Query: 586 VHLSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKP 645
           VHLSEDDQGLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC KA+E AQMEDKSKLGKP
Sbjct: 421 VHLSEDDQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKP 480

Query: 646 IKIKVKPENATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKK 705
           IKIKVKPE+  KE + KDDT+IH  L+N++ TVVEQ  EV+ DEA+ T  MKWLGSL+KK
Sbjct: 481 IKIKVKPESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKK 538

Query: 706 LLQVF 709
           LLQ+F
Sbjct: 541 LLQIF 538

[Close]
BLAST of Cp4.1LG16g08850 vs. ExPASy TrEMBL
Match: A0A5A7TDN6 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00570 PE=3 SV=1)

HSP 1 Score: 964 bits (2491), Expect = 0.0
Identity = 473/546 (86.63%), Postives = 497/546 (91.03%), Query Frame = 0 

Query: 166 VMLRLRVKP-VWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAV-HKASESY 225
           +MLRLRV P VWC++PISHFTSLPL PSILRP+LR H PRLF TRFSASSAV H  S+S 
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 226 SAKGLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 285
             K LSD D  PFLSCSMP YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 286 DFENFWSECPWDDDLKYAEAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 345
           DFENFWSECPWDDDLKYA+AVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 346 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMSIVLCMTVK 405
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDM       VK
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDM-------VK 240

Query: 406 DQTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEF 465
           DQTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEF
Sbjct: 241 DQTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEF 300

Query: 466 VARHIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVS 525
           VARHIGEDEGVILEAESGDFLGKHRGFWFYT+GQRQGL+LPGGPWYVVEKDI+NNVVFVS
Sbjct: 301 VARHIGEDEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVS 360

Query: 526 RNYFSLDKRRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVA 585
           RNYFS DKRRR+FRVGSLKWLSG+ PDQFT LQCKVRHGP FYSCNLTME NED  EDVA
Sbjct: 361 RNYFSFDKRRRVFRVGSLKWLSGMPPDQFTGLQCKVRHGPGFYSCNLTMEHNEDGHEDVA 420

Query: 586 VVHLSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGK 645
           VVHLSEDDQGLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC KA+E AQMEDKSKLGK
Sbjct: 421 VVHLSEDDQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGK 480

Query: 646 PIKIKVKPENATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRK 705
           PIKIKVKP++   E + KDDTKI    +N++ TVVEQ  +V+ DEA+ T PMKWLGSLRK
Sbjct: 481 PIKIKVKPDSGMNESKAKDDTKIQDESRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRK 539

Query: 706 KLLQVF 709
           KLLQ+F
Sbjct: 541 KLLQIF 539

[Close]
BLAST of Cp4.1LG16g08850 vs. ExPASy TrEMBL
Match: A0A5D3B8G8 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G001100 PE=3 SV=1)

HSP 1 Score: 961 bits (2483), Expect = 0.0
Identity = 471/546 (86.26%), Postives = 497/546 (91.03%), Query Frame = 0 

Query: 166 VMLRLRVKP-VWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAV-HKASESY 225
           +MLRLRV P VWC++PISHFTSLPL PSILRP+LR H PRLF TRFSASSAV H  S+S 
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 226 SAKGLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 285
             K LSD D  PFLSCSMP YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 286 DFENFWSECPWDDDLKYAEAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 345
           DFENFWSECPWDDDLKYA+AVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 346 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMSIVLCMTVK 405
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDM       VK
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDM-------VK 240

Query: 406 DQTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEF 465
           DQTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEF
Sbjct: 241 DQTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEF 300

Query: 466 VARHIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVS 525
           VARHIGEDEGVILEAESGDFLGKHRGFWFYT+GQRQGL+LPGGPWYVVEKDI+NNVVFVS
Sbjct: 301 VARHIGEDEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVS 360

Query: 526 RNYFSLDKRRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVA 585
           RNYFS DKRRR+FRVGSLKWLSG+ PD+FT LQCKVRHGP FYSCNLTME N+D  EDVA
Sbjct: 361 RNYFSFDKRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVA 420

Query: 586 VVHLSEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGK 645
           VVHLSEDDQGLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC KA+E AQMEDKSKLGK
Sbjct: 421 VVHLSEDDQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGK 480

Query: 646 PIKIKVKPENATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRK 705
           PIKIKVKP++   E + KDDTKI    +N++ TVVEQ  +V+ DEA+ T PMKWLGSLRK
Sbjct: 481 PIKIKVKPDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRK 539

Query: 706 KLLQVF 709
           KLLQ+F
Sbjct: 541 KLLQIF 539

[Close]
BLAST of Cp4.1LG16g08850 vs. TAIR 10
Match: AT1G51310.1 (transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases )

HSP 1 Score: 707.6 bits (1825), Expect = 1.0e-203
Identity = 336/427 (78.69%), Postives = 372/427 (87.12%), Query Frame = 0 

Query: 233 LEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSEC 292
           ++ +LSCSMP  PL+VAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE FENFW++C
Sbjct: 69  VDQYLSCSMPEKPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWNQC 128

Query: 293 PWDDDLKYAEAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFG 352
           PW+DDLKYA+ VC+QVDVPLEVVHLT+EYW  VVSYIIEEYRCGRTPNPDVLCNTRIKFG
Sbjct: 129 PWEDDLKYAKHVCEQVDVPLEVVHLTDEYWERVVSYIIEEYRCGRTPNPDVLCNTRIKFG 188

Query: 353 AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMSIVLCMTVKDQTYFLSHL 412
           AFMDAIS+ME+DYV SGHYA V+HP ADQ D  SVLELS+DM       VKDQTYFLSHL
Sbjct: 189 AFMDAISDMEYDYVGSGHYAKVVHPPADQNDASSVLELSQDM-------VKDQTYFLSHL 248

Query: 413 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 472
           SQ QLKRLLFPLGC+ KDEVRKLA +F+LPNKDRKDSQGICFLGKIKFS+FV RHIGE E
Sbjct: 249 SQTQLKRLLFPLGCVKKDEVRKLATQFDLPNKDRKDSQGICFLGKIKFSDFVCRHIGEME 308

Query: 473 GVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKR 532
           G+ILEAESGDFLG HRGFWFYTIGQRQGL+LPGGPWYVVEKD +NNVVFVSRNY+S+DKR
Sbjct: 309 GIILEAESGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKR 368

Query: 533 RRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDDQ 592
           RR+FRVGSL+W SG    +  EL+CKVRHGP FYSC+  ME     D DVAVVHL EDDQ
Sbjct: 369 RRIFRVGSLRWNSGKPSGKVRELRCKVRHGPGFYSCSFEME----GDGDVAVVHLDEDDQ 428

Query: 593 GLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPE 652
           GLAAGQFAAFYEG  CIGSGVILESWDDQ FPVCAKAL++A +EDK+KLGKP+KI   P 
Sbjct: 429 GLAAGQFAAFYEGTTCIGSGVILESWDDQCFPVCAKALQLAALEDKTKLGKPVKIMTMPT 484

Query: 653 NATKECE 660
            ++ E +
Sbjct: 489 MSSVEAD 484

The following BLAST results are available for this feature:
BLAST of Cp4.1LG16g08850 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O516256.6e-9945.70tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / ... [more]
B7J2N76.6e-9945.70tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) OX=44598... [more]
Q0SMH13.3e-9845.70tRNA-specific 2-thiouridylase MnmA OS=Borrelia afzelii (strain PKo) OX=390236 GN... [more]
Q660I84.3e-9845.16tRNA-specific 2-thiouridylase MnmA OS=Borrelia bavariensis (strain ATCC BAA-2496... [more]
B2S1253.0e-9645.58tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) OX=314... [more]
BLAST of Cp4.1LG16g08850 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023513286.10.098.71uncharacterized protein LOC111777800 [Cucurbita pepo subsp. pepo][more]
XP_022944568.10.097.79uncharacterized protein LOC111448983 [Cucurbita moschata][more]
KAG6570391.10.097.42hypothetical protein SDJN03_29306, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7010265.10.091.41mnmA [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022985745.10.096.50uncharacterized protein LOC111483706 [Cucurbita maxima][more]
BLAST of Cp4.1LG16g08850 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1FY630.097.79tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1JC610.096.50tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A0A0KX160.088.26tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis sativus OX=3659 GN=Cs... [more]
A0A5A7TDN60.086.63tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1... [more]
A0A5D3B8G80.086.26tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1... [more]
BLAST of Cp4.1LG16g08850 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G51310.11.0e-20378.69transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 97..172
e-value: 1.3E-10
score: 43.0
coord: 238..466
e-value: 4.6E-82
score: 276.7
IPR023382Adenine nucleotide alpha hydrolase-like domainsGENE3D2.30.30.280coord: 467..526
e-value: 2.0E-18
score: 67.5
IPR004506tRNA-specific 2-thiouridylaseTIGRFAMTIGR00420TIGR00420coord: 247..614
e-value: 1.1E-106
score: 354.6
IPR004506tRNA-specific 2-thiouridylaseHAMAPMF_00144tRNA_thiouridyl_MnmAcoord: 246..615
score: 32.472
IPR004506tRNA-specific 2-thiouridylaseCDDcd01998tRNA_Me_transcoord: 247..614
e-value: 1.25781E-155
score: 452.361
NoneNo IPR availablePFAMPF03054tRNA_Me_transcoord: 106..141
e-value: 2.1E-8
score: 33.3
coord: 247..614
e-value: 4.2E-123
score: 410.6
NoneNo IPR availableGENE3D2.40.30.10Translation factorscoord: 533..622
e-value: 1.6E-13
score: 52.0
NoneNo IPR availablePANTHERPTHR43052FAMILY NOT NAMEDcoord: 223..647
coord: 82..141
NoneNo IPR availableCDDcd01986Alpha_ANH_likecoord: 107..128
e-value: 9.06147E-4
score: 37.4836
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 106..141
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 247..499

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG16g08850.1Cp4.1LG16g08850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008033 tRNA processing
molecular_function GO:0005524 ATP binding
molecular_function GO:0061708 tRNA-5-taurinomethyluridine 2-sulfurtransferase
molecular_function GO:0000049 tRNA binding
molecular_function GO:0016783 sulfurtransferase activity
molecular_function GO:0016740 transferase activity