Cp4.1LG16g04770 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG16g04770 vs. ExPASy Swiss-Prot
Match: Q9ZNS2 (Indole-3-acetic acid-amido synthetase GH3.10 OS=Arabidopsis thaliana OX=3702 GN=GH3.10 PE=2 SV=1)

HSP 1 Score: 786.2 bits (2029), Expect = 2.7e-226
Identity = 385/588 (65.48%), Postives = 457/588 (77.72%), Query Frame = 0

Query: 41  EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 100
           +D++ WFE VS  A   Q  TLR IL  N GVEYL +WLG V++ ++ DD+ LE+LFTSL
Sbjct: 9   DDVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKM-DDYTLETLFTSL 68

Query: 101 VPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 160
           VP+ SHAD +P++QRIADG++SPLLTQ+PI  LSLSSGTTEGRQKYVPFT HSAQTTL I
Sbjct: 69  VPIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQTTLQI 128

Query: 161 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 220
           FR++AAYRSR YP REGGRILEFIY+ K+ KT GG+T GTATTHYYASE+FK KQ  TKS
Sbjct: 129 FRLSAAYRSRFYPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYYASEEFKTKQETTKS 188

Query: 221 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 280
           FTCSPQEVISGGD+ Q TYCHLLLGL +S QVEFV S F+YTIVQAF+  E+ W +IC D
Sbjct: 189 FTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQAFSFFEEIWREICAD 248

Query: 281 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQ-DWLDLVPKLWPNCKYV 340
           I    L SRITLP++RKAVL +I PNP L + I  +C ELE    W  L+ KLWPN K++
Sbjct: 249 IKEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLGWFGLISKLWPNAKFI 308

Query: 341 YSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYF 400
            SIMTGS+ PYL KLR YAG LPLVS DYGSTESWIGVNVDP LPPE+V+FAV+PTFSYF
Sbjct: 309 SSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLPPEDVSFAVIPTFSYF 368

Query: 401 EFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTG--------- 460
           EFIPL+R+  Q D     +    DF+E +P+PLSQVK+GQ+YELVLTTFTG         
Sbjct: 369 EFIPLYRRQNQSD-----ICIDGDFVEDKPVPLSQVKLGQEYELVLTTFTGLYRYRLGDV 428

Query: 461 -----FHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSH 520
                FH  TPKL+FI RRKL+LT+NIDKNTEKDLQ VV++ SQLL+  + AE+VDFTSH
Sbjct: 429 VEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQLLSRSTRAEVVDFTSH 488

Query: 521 ADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVER 580
           AD+  +PGHYVI+WE++G  +D  L  CC EMD AFVDYGYVVSRR N+IGPLELR+VER
Sbjct: 489 ADVIARPGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSRRMNSIGPLELRVVER 548

Query: 581 GSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 614
           G+F K+ E  +G    L+QFKTPRCTTN V+L ILN ST+K F S+AY
Sbjct: 549 GTFGKVAERCVGKCGGLNQFKTPRCTTNSVMLDILNDSTIKRFRSSAY 590

BLAST of Cp4.1LG16g04770 vs. ExPASy Swiss-Prot
Match: A0A1J6KGJ9 (Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 PE=1 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 7.5e-160
Identity = 282/589 (47.88%), Postives = 396/589 (67.23%), Query Frame = 0

Query: 41  EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 100
           E++++ FE ++ +AG  Q  TL++IL +N G EYL QW     ++   D  +    F + 
Sbjct: 14  EEVIEEFEVLTKDAGKIQEETLQKILEENGGTEYLQQW----GLNGKTDSLS----FKNC 73

Query: 101 VPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 160
           +P+ +H D EP++ RIADGD SP+LT +PI T+SLSSGTT+G+ K+VPF     ++T+ I
Sbjct: 74  IPIVTHKDLEPYIHRIADGDLSPILTGKPITTISLSSGTTQGKPKFVPFNEELMESTMQI 133

Query: 161 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 220
           F+ +  +R+R +P    G+ L+FIY SKQ KTKGG+  GTATT+ Y + QFK      ++
Sbjct: 134 FKTSFVFRNREFPV-VNGKALQFIYGSKQFKTKGGLAAGTATTNVYRNAQFKKTMKAMQT 193

Query: 221 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 280
             CSP EVI G D++QS YCHLL GL F D+V+ V+STFA++IV AF   E  W ++  +
Sbjct: 194 PCCSPDEVIFGPDFQQSLYCHLLCGLIFRDEVQVVSSTFAHSIVHAFRNFEQIWQELVTN 253

Query: 281 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCKYVY 340
           I    L SR+ +P +R A+  ++ P+P L   I   C  L   +W  L+P+L+PN +Y+Y
Sbjct: 254 IREGVLSSRVIVPSMRAAMSKLLKPDPELADTIFNKCSRL--SNWYGLIPELFPNTRYIY 313

Query: 341 SIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYFE 400
            IMTGS++PYLKKLR YAG LPL+S DYGS+E WIG NV+P LPPE VT+AV+P   YFE
Sbjct: 314 GIMTGSMEPYLKKLRHYAGDLPLLSADYGSSEGWIGANVNPELPPELVTYAVLPNIGYFE 373

Query: 401 FIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFT----------- 460
           FIPL                  D LE  P+ L++VK+G++YE+V+T F            
Sbjct: 374 FIPLMENL--------------DGLEPMPVGLTEVKLGEEYEIVVTNFAGLYRYRLGDVV 433

Query: 461 ---GFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSHA 520
              GFHN TP+L FICRR L+L++NIDKNTEKDLQL VE  +++L+D+   E+VDFTSH 
Sbjct: 434 KIKGFHNGTPELQFICRRNLLLSINIDKNTEKDLQLAVEAAAKILSDEK-LEVVDFTSHV 493

Query: 521 DLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVERG 580
           +++  PGHYVIFWE+ G   + +L  CC+ +D +FVD GYV SR+ + IG LELRIV+RG
Sbjct: 494 NVSADPGHYVIFWELNGEASEEILKECCNCLDKSFVDAGYVGSRKVHAIGALELRIVKRG 553

Query: 581 SFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAY 614
           +F+KI++H++G GAA+SQFKTPRC   TN  +L IL+ + V+++ STA+
Sbjct: 554 TFHKILDHFVGLGAAVSQFKTPRCVGPTNLSVLQILSSNVVESYFSTAF 576

BLAST of Cp4.1LG16g04770 vs. ExPASy Swiss-Prot
Match: A0A314KSQ4 (Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 PE=1 SV=2)

HSP 1 Score: 560.1 bits (1442), Expect = 3.2e-158
Identity = 286/589 (48.56%), Postives = 388/589 (65.87%), Query Frame = 0

Query: 41  EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 100
           E +++ FE ++ +AG  Q  TL++IL QN G EYL  W   +N    P        F + 
Sbjct: 15  EKVIEEFEDLTKDAGKIQEETLKKILEQNGGTEYLQLW--GLNGRTDP------QTFKNC 74

Query: 101 VPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 160
           VP+ +H D EP++QRIADGD SP+LT +PI+T+SLSSGTT+G+ K+VPF     ++T+ I
Sbjct: 75  VPIVTHNDLEPYIQRIADGDLSPILTGKPIETISLSSGTTQGKPKFVPFNDELMESTMQI 134

Query: 161 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 220
           F+ + A+R+R +P    G+ L+FIYSSKQ KTKGG+  GTATT+ Y + QFK       +
Sbjct: 135 FKTSFAFRNREFPI-GNGKALQFIYSSKQFKTKGGLAAGTATTNVYRNAQFKKTMKAMCT 194

Query: 221 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 280
             CSP EVI G D+ QS YCHLL GL F D+V+ V+STFA++IV AF   E  W  +  D
Sbjct: 195 PCCSPDEVIFGPDFHQSLYCHLLCGLIFHDEVQVVSSTFAHSIVHAFRTFEQVWEALVVD 254

Query: 281 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCKYVY 340
           I    L SR+T+P IR A+  ++ P+P L   I   C  L   +W  L+P L+PN +Y+Y
Sbjct: 255 IREGVLSSRVTVPSIRLAMSKLLKPDPELADTIYNKCSRL--SNWYGLIPDLFPNTRYIY 314

Query: 341 SIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYFE 400
            IMTGS++PYLKKLR YAG LPL+S DYGS+E W+GVNV+P LPPE VT+AV+P   YFE
Sbjct: 315 GIMTGSMEPYLKKLRHYAGELPLLSADYGSSEGWVGVNVNPKLPPELVTYAVLPNIGYFE 374

Query: 401 FIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFT----------- 460
           FIPL       +  +S            P+ L++VK+G++YE+V T F            
Sbjct: 375 FIPLGGNLNGIEQANS------------PVGLTEVKLGEEYEVVFTNFAGLYRYRLGDVV 434

Query: 461 ---GFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSHA 520
              GFHN TP+L F+CR  L+L++NIDKNTEKDLQL VE  ++ L D+   E+VDFTSH 
Sbjct: 435 KVKGFHNGTPELQFVCRSNLLLSINIDKNTEKDLQLAVEAAAKRLVDEK-LEVVDFTSHV 494

Query: 521 DLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVERG 580
           +++  PGHYVIFWE+ G   D +L  CC+ +D +F+D GYV SR+ N IG LELRIV+RG
Sbjct: 495 NVSADPGHYVIFWELSGEATDEMLQDCCNCLDRSFIDAGYVSSRKVNAIGALELRIVKRG 554

Query: 581 SFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAY 614
           +F+KI++H++G G A+SQFKTPRC    N  LL IL+ + V+ + STA+
Sbjct: 555 TFHKILDHFVGLGGAVSQFKTPRCVGPKNSSLLQILSSNVVETYVSTAF 579

BLAST of Cp4.1LG16g04770 vs. ExPASy Swiss-Prot
Match: Q6I581 (Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 2.3e-153
Identity = 275/591 (46.53%), Postives = 380/591 (64.30%), Query Frame = 0

Query: 41  EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYL-NQWLGDVNIHQIPDDFALESLFTS 100
           E+ ++ FE ++ +A   Q  TL++IL  N   EYL N  LG     +          + S
Sbjct: 7   EETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNFGLGGRTDAE---------SYKS 66

Query: 101 LVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLL 160
            +PL  H D EP++QRI DGD+SP++T +PI  LSLSSGTT G+ K++PF     +TTL 
Sbjct: 67  CIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKPKFIPFNDELLETTLQ 126

Query: 161 IFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTK 220
           I+R + A+R+R YP  + G+ L+F+Y SKQ  TKGGI   TATT+ Y  +++K      +
Sbjct: 127 IYRTSYAFRNREYPIGQ-GKALQFVYGSKQVITKGGILATTATTNLYRRQRYKEGMKDIQ 186

Query: 221 SFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICH 280
           S  CSP EVI G D+ QS YCHLL GL +S++V  V STFA+++V AF   E+ W  +C 
Sbjct: 187 SQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAHSLVHAFQTFEEVWEDLCT 246

Query: 281 DITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCKYV 340
           DI    L  ++T P IR+AV  I+ PNP L   I   C  L   +W  ++P LWPN KYV
Sbjct: 247 DIRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIGL--SNWYGVIPALWPNAKYV 306

Query: 341 YSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYF 400
           Y IMTGS++PYLKKLR YAG LPL+S DYG++E W+G N+DP +PPE VT+AV+P   YF
Sbjct: 307 YGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNIDPTVPPEQVTYAVLPQVGYF 366

Query: 401 EFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTG--------- 460
           EFIPL +   +    S+S+     ++E  P+ L++V++G+ YE+V+T F G         
Sbjct: 367 EFIPLEKPIGEETENSASI----HYIESDPVGLTEVEVGKIYEVVITNFAGLYRYRLGDV 426

Query: 461 -----FHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSH 520
                FHN TP+L FICRR LVL++NIDKNTEKDLQL VE  S+ L  +   E++DFTS 
Sbjct: 427 VKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVEEASKFLEGEK-LEVMDFTSF 486

Query: 521 ADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVER 580
            + ++ PG YVIFWE+ G   D VL  C + +D AF+D GY  SR+  TIGPLELRI+ +
Sbjct: 487 VERSSDPGRYVIFWELSGDASDEVLSSCANALDLAFIDAGYTGSRKIKTIGPLELRILRK 546

Query: 581 GSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAYG 615
           G+F +I++H++  G A+SQFKTPR    +N  +L IL+ +  +++ STAYG
Sbjct: 547 GTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQILSRNVTQSYFSTAYG 580

BLAST of Cp4.1LG16g04770 vs. ExPASy Swiss-Prot
Match: Q9SKE2 (Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 PE=1 SV=2)

HSP 1 Score: 516.9 bits (1330), Expect = 3.1e-145
Identity = 265/599 (44.24%), Postives = 374/599 (62.44%), Query Frame = 0

Query: 36  MFEEVED-----MVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDD 95
           M E+VE      ++D F+ ++  A   Q +TL+EIL +N    YL       N     + 
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQ------NCGLNGNA 60

Query: 96  FALESLFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFT 155
              E  F S+VPL +  + EP+++R+ DGD+SP+LT  P+  +SLSSGT++GR K++PFT
Sbjct: 61  TDPEEAFKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFT 120

Query: 156 PHSAQTTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQ 215
               + TL +FR A A+R+R +P  + G+ L+FI+SSKQ  + GG+  GTATT+ Y +  
Sbjct: 121 DELMENTLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPN 180

Query: 216 FKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNEL 275
           FK       S +CSP EVI   D  Q+ YCHLL G+ F DQV++V + FA+ +V AF   
Sbjct: 181 FKAGMKSITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTF 240

Query: 276 EDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVP 335
           E  W +I  DI    L +RIT+P +R A+  +++PNP L   I   C  L   +W  L+P
Sbjct: 241 EQVWEEIVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIP 300

Query: 336 KLWPNCKYVYSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTF 395
            L+PN KYVY IMTGS++PY+ KLR YAG LPLVS DYGS+E WI  NV P L PE  TF
Sbjct: 301 ALFPNAKYVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATF 360

Query: 396 AVVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFT- 455
           AV+P   YFEF+P+       + G           E +P+ L+QVKIG++YE+V+T +  
Sbjct: 361 AVIPNLGYFEFLPV------SETGEG---------EEKPVGLTQVKIGEEYEVVITNYAG 420

Query: 456 -------------GFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSG 515
                        GF+N TP+L FICRR L+L++NIDKNTE+DLQL VE  ++ L+++  
Sbjct: 421 LYRYRLGDVVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK- 480

Query: 516 AELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIG 575
            E++DF+S+ D++  PGHY IFWE+ G   + VL  CC+ +D AF+D GYV SR+  TIG
Sbjct: 481 IEVIDFSSYIDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIG 540

Query: 576 PLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAY 614
            LELR+V +G+F KI EH++G G++  QFK PRC   +N  +L IL  + V ++ STA+
Sbjct: 541 ALELRVVAKGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

BLAST of Cp4.1LG16g04770 vs. NCBI nr
Match: XP_023512179.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1171 bits (3030), Expect = 0.0
Identity = 579/593 (97.64%), Postives = 579/593 (97.64%), Query Frame = 0

Query: 36  MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 95
           MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 96  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 155
           LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 156 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 215
           TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ
Sbjct: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180

Query: 216 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 275
           LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP
Sbjct: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240

Query: 276 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 335
           QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN
Sbjct: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 300

Query: 336 CKYVYSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 395
           CKYVYSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT
Sbjct: 301 CKYVYSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 360

Query: 396 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTG----- 455
           FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTG     
Sbjct: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420

Query: 456 ---------FHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 515
                    FHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD
Sbjct: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480

Query: 516 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 575
           FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR
Sbjct: 481 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540

Query: 576 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 614
           IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG
Sbjct: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 593

BLAST of Cp4.1LG16g04770 vs. NCBI nr
Match: KAG6570848.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1170 bits (3026), Expect = 0.0
Identity = 577/593 (97.30%), Postives = 579/593 (97.64%), Query Frame = 0

Query: 36  MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 95
           MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 96  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 155
           LFTSL+PLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 156 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 215
           TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ
Sbjct: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180

Query: 216 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 275
           LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP
Sbjct: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240

Query: 276 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 335
           QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN
Sbjct: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 300

Query: 336 CKYVYSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 395
           CKYVYSIMTGS+QPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT
Sbjct: 301 CKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 360

Query: 396 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTG----- 455
           FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTG     
Sbjct: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420

Query: 456 ---------FHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 515
                    FHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD
Sbjct: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480

Query: 516 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 575
           FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR
Sbjct: 481 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540

Query: 576 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 614
           IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG
Sbjct: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 593

BLAST of Cp4.1LG16g04770 vs. NCBI nr
Match: XP_022943441.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita moschata])

HSP 1 Score: 1167 bits (3019), Expect = 0.0
Identity = 575/593 (96.96%), Postives = 579/593 (97.64%), Query Frame = 0

Query: 36  MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 95
           MFE+VEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 96  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 155
           LFTSL+PLSSHADFEPFLQRIADGDSSPLLTQQPI+TLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 156 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 215
           TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ
Sbjct: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180

Query: 216 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 275
           LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP
Sbjct: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240

Query: 276 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 335
           QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN
Sbjct: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 300

Query: 336 CKYVYSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 395
           CKYVYSIMTGS+QPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT
Sbjct: 301 CKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 360

Query: 396 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTG----- 455
           FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTG     
Sbjct: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420

Query: 456 ---------FHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 515
                    FHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD
Sbjct: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480

Query: 516 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 575
           FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR
Sbjct: 481 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540

Query: 576 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 614
           IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG
Sbjct: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 593

BLAST of Cp4.1LG16g04770 vs. NCBI nr
Match: KAG7010696.1 (Indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1155 bits (2988), Expect = 0.0
Identity = 575/608 (94.57%), Postives = 577/608 (94.90%), Query Frame = 0

Query: 36  MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 95
           MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 96  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 155
           LFTSL+PLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 156 TTLLIFRIAAAYRSR---------------VYPTREGGRILEFIYSSKQTKTKGGITTGT 215
           TTLLIFRIAAAYRSR               VYPTREGGRILEFIYSSKQTKTKGGITTGT
Sbjct: 121 TTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGT 180

Query: 216 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 275
           ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA
Sbjct: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240

Query: 276 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEEL 335
           YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKI AVCEEL
Sbjct: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL 300

Query: 336 ERQDWLDLVPKLWPNCKYVYSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD 395
           ERQDWLDLVPKLWPNCKYVYSIMTGS+QPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD
Sbjct: 301 ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD 360

Query: 396 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQ 455
           PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVA FDDFLEGQPLPLSQVKIGQQ
Sbjct: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQ 420

Query: 456 YELVLTTFTG--------------FHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 515
           YELVLTTFTG              FHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER
Sbjct: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480

Query: 516 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY 575
           GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY
Sbjct: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY 540

Query: 576 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 614
           VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK
Sbjct: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 600

BLAST of Cp4.1LG16g04770 vs. NCBI nr
Match: XP_022986272.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita maxima])

HSP 1 Score: 1152 bits (2979), Expect = 0.0
Identity = 570/593 (96.12%), Postives = 574/593 (96.80%), Query Frame = 0

Query: 36  MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 95
           MFEEVEDMVDWFERVSSEAGVAQ RTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 96  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 155
           LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 156 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 215
           TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ
Sbjct: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180

Query: 216 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 275
           LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP
Sbjct: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240

Query: 276 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 335
           QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKI AVCEELERQDWLDLVPKLW N
Sbjct: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSN 300

Query: 336 CKYVYSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 395
           CKYVYSIMTGS+QPYLKKLRRYAG LPLVSGDYGSTESWIG+NVDPCLPPENVTFAVVPT
Sbjct: 301 CKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPT 360

Query: 396 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTG----- 455
           FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTG     
Sbjct: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420

Query: 456 ---------FHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 515
                    FHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD
Sbjct: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480

Query: 516 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 575
           FTSHADLTNQPGHYVIFWEVKG+V+DAVLG CCSEMDAAFVDYGYVVSRRSNTIGPLELR
Sbjct: 481 FTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540

Query: 576 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 614
           IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG
Sbjct: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 593

BLAST of Cp4.1LG16g04770 vs. ExPASy TrEMBL
Match: A0A6J1FRQ1 (indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita moschata OX=3662 GN=LOC111448207 PE=3 SV=1)

HSP 1 Score: 1167 bits (3019), Expect = 0.0
Identity = 575/593 (96.96%), Postives = 579/593 (97.64%), Query Frame = 0

Query: 36  MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 95
           MFE+VEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 96  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 155
           LFTSL+PLSSHADFEPFLQRIADGDSSPLLTQQPI+TLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 156 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 215
           TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ
Sbjct: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180

Query: 216 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 275
           LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP
Sbjct: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240

Query: 276 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 335
           QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN
Sbjct: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 300

Query: 336 CKYVYSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 395
           CKYVYSIMTGS+QPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT
Sbjct: 301 CKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 360

Query: 396 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTG----- 455
           FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTG     
Sbjct: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420

Query: 456 ---------FHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 515
                    FHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD
Sbjct: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480

Query: 516 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 575
           FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR
Sbjct: 481 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540

Query: 576 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 614
           IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG
Sbjct: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 593

BLAST of Cp4.1LG16g04770 vs. ExPASy TrEMBL
Match: A0A6J1J741 (indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita maxima OX=3661 GN=LOC111484069 PE=3 SV=1)

HSP 1 Score: 1152 bits (2979), Expect = 0.0
Identity = 570/593 (96.12%), Postives = 574/593 (96.80%), Query Frame = 0

Query: 36  MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 95
           MFEEVEDMVDWFERVSSEAGVAQ RTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 96  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 155
           LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 156 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 215
           TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ
Sbjct: 121 TTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 180

Query: 216 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 275
           LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP
Sbjct: 181 LKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWP 240

Query: 276 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPN 335
           QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKI AVCEELERQDWLDLVPKLW N
Sbjct: 241 QICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSN 300

Query: 336 CKYVYSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPT 395
           CKYVYSIMTGS+QPYLKKLRRYAG LPLVSGDYGSTESWIG+NVDPCLPPENVTFAVVPT
Sbjct: 301 CKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPT 360

Query: 396 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTG----- 455
           FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTG     
Sbjct: 361 FSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCR 420

Query: 456 ---------FHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 515
                    FHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD
Sbjct: 421 LGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVD 480

Query: 516 FTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELR 575
           FTSHADLTNQPGHYVIFWEVKG+V+DAVLG CCSEMDAAFVDYGYVVSRRSNTIGPLELR
Sbjct: 481 FTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELR 540

Query: 576 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 614
           IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG
Sbjct: 541 IVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 593

BLAST of Cp4.1LG16g04770 vs. ExPASy TrEMBL
Match: A0A5D3B9T4 (Jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold464G00300 PE=3 SV=1)

HSP 1 Score: 972 bits (2513), Expect = 0.0
Identity = 478/597 (80.07%), Postives = 529/597 (88.61%), Query Frame = 0

Query: 32  EVRTMFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDF 91
           ++  + EE ED++DWFE+VSSEA VAQ RTLR+IL+QN GVEYL +W+GDVNIH+IPDDF
Sbjct: 2   KIDELCEEEEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDDF 61

Query: 92  ALESLFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTP 151
            L+S+FTSL+PLSSHA FEPFLQRIADGDSSPLLTQQPI TLSLSSGTTEGRQKYVPFTP
Sbjct: 62  TLQSIFTSLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTP 121

Query: 152 HSAQTTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQF 211
           HSA TTLLIFR+AAAYRSRVYPTREGG+ILEFIYSSKQTKTKGGITTGTATTHYYASE+F
Sbjct: 122 HSAHTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEF 181

Query: 212 KIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELE 271
           KIKQLKTKSFTCSPQEVISG DYKQSTYCHLLLGL +S++VEFVTSTFAYTIVQAFN+LE
Sbjct: 182 KIKQLKTKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQAFNQLE 241

Query: 272 DCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPK 331
           + W +I HDI+HATL SRI +PEIRKAVL ++SP P LG KI  VCEEL+++ WL L+PK
Sbjct: 242 ESWEEIIHDISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKKEGWLGLIPK 301

Query: 332 LWPNCKYVYSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFA 391
           LWPNCKYVYSIMTGS+QPYLKKLR Y G LPLVSGDYGSTESWIGVNVDP LPPENVTFA
Sbjct: 302 LWPNCKYVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFA 361

Query: 392 VVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTG- 451
           V+PTFSYFEFIPLF+QH      SS+  A  DFLEGQP+ LS+VKIGQQYELVLTTFTG 
Sbjct: 362 VIPTFSYFEFIPLFKQHATT---SSTDTAIHDFLEGQPIALSEVKIGQQYELVLTTFTGL 421

Query: 452 -------------FHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGA 511
                        FHNKTPKLNFICRRKLVLTVNIDKNTEKD+QL VERGSQL++  SGA
Sbjct: 422 YRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQLMSQLSGA 481

Query: 512 ELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGP 571
           ELVDFTS+A+L+NQPGHYVIFWEVKG V D VLG CCSEMDAAFVDYGYVVSR++N+IGP
Sbjct: 482 ELVDFTSYAELSNQPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVVSRKANSIGP 541

Query: 572 LELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 614
           LELRIVERGSFNKI+E+YIGNGAALSQFKTPRCTTN  LL ILNLST+++F STAY 
Sbjct: 542 LELRIVERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSFFSTAYA 595

BLAST of Cp4.1LG16g04770 vs. ExPASy TrEMBL
Match: A0A1S3BIL6 (jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490483 PE=3 SV=1)

HSP 1 Score: 972 bits (2513), Expect = 0.0
Identity = 478/597 (80.07%), Postives = 529/597 (88.61%), Query Frame = 0

Query: 32  EVRTMFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDF 91
           ++  + EE ED++DWFE+VSSEA VAQ RTLR+IL+QN GVEYL +W+GDVNIH+IPDDF
Sbjct: 2   KIDELCEEEEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDDF 61

Query: 92  ALESLFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTP 151
            L+S+FTSL+PLSSHA FEPFLQRIADGDSSPLLTQQPI TLSLSSGTTEGRQKYVPFTP
Sbjct: 62  TLQSIFTSLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTP 121

Query: 152 HSAQTTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQF 211
           HSA TTLLIFR+AAAYRSRVYPTREGG+ILEFIYSSKQTKTKGGITTGTATTHYYASE+F
Sbjct: 122 HSAHTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEF 181

Query: 212 KIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELE 271
           KIKQLKTKSFTCSPQEVISG DYKQSTYCHLLLGL +S++VEFVTSTFAYTIVQAFN+LE
Sbjct: 182 KIKQLKTKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQAFNQLE 241

Query: 272 DCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPK 331
           + W +I HDI+HATL SRI +PEIRKAVL ++SP P LG KI  VCEEL+++ WL L+PK
Sbjct: 242 ESWEEIIHDISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKKEGWLGLIPK 301

Query: 332 LWPNCKYVYSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFA 391
           LWPNCKYVYSIMTGS+QPYLKKLR Y G LPLVSGDYGSTESWIGVNVDP LPPENVTFA
Sbjct: 302 LWPNCKYVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFA 361

Query: 392 VVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTG- 451
           V+PTFSYFEFIPLF+QH      SS+  A  DFLEGQP+ LS+VKIGQQYELVLTTFTG 
Sbjct: 362 VIPTFSYFEFIPLFKQHATT---SSTDTAIHDFLEGQPIALSEVKIGQQYELVLTTFTGL 421

Query: 452 -------------FHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGA 511
                        FHNKTPKLNFICRRKLVLTVNIDKNTEKD+QL VERGSQL++  SGA
Sbjct: 422 YRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQLMSQLSGA 481

Query: 512 ELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGP 571
           ELVDFTS+A+L+NQPGHYVIFWEVKG V D VLG CCSEMDAAFVDYGYVVSR++N+IGP
Sbjct: 482 ELVDFTSYAELSNQPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVVSRKANSIGP 541

Query: 572 LELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 614
           LELRIVERGSFNKI+E+YIGNGAALSQFKTPRCTTN  LL ILNLST+++F STAY 
Sbjct: 542 LELRIVERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSFFSTAYA 595

BLAST of Cp4.1LG16g04770 vs. ExPASy TrEMBL
Match: A0A0A0KE57 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G124120 PE=3 SV=1)

HSP 1 Score: 958 bits (2477), Expect = 0.0
Identity = 471/591 (79.70%), Postives = 521/591 (88.16%), Query Frame = 0

Query: 38  EEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLF 97
           E  ED++ WFE+VSSEA +AQ RTLR+IL+QN GVEYL +W+GDVNIH+IPDDF L S+F
Sbjct: 2   EGEEDIIGWFEQVSSEAALAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDDFTLHSIF 61

Query: 98  TSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTT 157
           TS +PLSSHA FEPFLQRIADGDSSPLLTQQPI TLSLSSGTTEGRQKYVPFTPHSAQTT
Sbjct: 62  TSSIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTPHSAQTT 121

Query: 158 LLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLK 217
           LLIFR+AAAYRSRVYP R+GG+ILEFIYSSKQTKTKGGITTGTATTHYYASE+FKIKQLK
Sbjct: 122 LLIFRLAAAYRSRVYPIRDGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLK 181

Query: 218 TKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQI 277
           TKSFTCSPQEVI+G DYKQSTYCHLLLGL +S++VEFVTSTFAYTIVQAFN+LE+ W ++
Sbjct: 182 TKSFTCSPQEVITGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQAFNQLEESWEEM 241

Query: 278 CHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCK 337
            HD++HATL SRI +PEIRKAVL ++SP P LG KI  VCEEL ++ WL L+PKLWPNCK
Sbjct: 242 IHDLSHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELMKEGWLGLIPKLWPNCK 301

Query: 338 YVYSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFS 397
           YVYSIMTGS+QPYLKKLR Y G LPLVSGDYGSTESWIGVNVDP LPPENVTFAV+PTFS
Sbjct: 302 YVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPTFS 361

Query: 398 YFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTG------- 457
           YFEFIPLF+QH      SS+  A  DFLEGQP+ LS+VKIGQQYELVLTTFTG       
Sbjct: 362 YFEFIPLFKQHATT---SSADTAIHDFLEGQPIALSEVKIGQQYELVLTTFTGLYRCRLG 421

Query: 458 -------FHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFT 517
                  FHNKTPKLNFICRRKLVLTVNIDKNTEKD+QL VERGSQL++  SGAELVDFT
Sbjct: 422 DVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQLMSQLSGAELVDFT 481

Query: 518 SHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIV 577
           S+A+L+NQPGHYVIFWE+KG V D VLG CCSEMDAAFVDYGYVVSR++N+IGPLELRIV
Sbjct: 482 SYAELSNQPGHYVIFWELKGDVNDKVLGMCCSEMDAAFVDYGYVVSRKANSIGPLELRIV 541

Query: 578 ERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG 614
           ERGSFNKI+EHYIGNGAALSQFKTPRCTTN  LL ILNLST+K+F STAY 
Sbjct: 542 ERGSFNKILEHYIGNGAALSQFKTPRCTTNHFLLNILNLSTLKSFFSTAYA 589

BLAST of Cp4.1LG16g04770 vs. TAIR 10
Match: AT4G03400.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 786.2 bits (2029), Expect = 1.9e-227
Identity = 385/588 (65.48%), Postives = 457/588 (77.72%), Query Frame = 0

Query: 41  EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 100
           +D++ WFE VS  A   Q  TLR IL  N GVEYL +WLG V++ ++ DD+ LE+LFTSL
Sbjct: 9   DDVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKM-DDYTLETLFTSL 68

Query: 101 VPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 160
           VP+ SHAD +P++QRIADG++SPLLTQ+PI  LSLSSGTTEGRQKYVPFT HSAQTTL I
Sbjct: 69  VPIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQTTLQI 128

Query: 161 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 220
           FR++AAYRSR YP REGGRILEFIY+ K+ KT GG+T GTATTHYYASE+FK KQ  TKS
Sbjct: 129 FRLSAAYRSRFYPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYYASEEFKTKQETTKS 188

Query: 221 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 280
           FTCSPQEVISGGD+ Q TYCHLLLGL +S QVEFV S F+YTIVQAF+  E+ W +IC D
Sbjct: 189 FTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQAFSFFEEIWREICAD 248

Query: 281 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQ-DWLDLVPKLWPNCKYV 340
           I    L SRITLP++RKAVL +I PNP L + I  +C ELE    W  L+ KLWPN K++
Sbjct: 249 IKEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLGWFGLISKLWPNAKFI 308

Query: 341 YSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYF 400
            SIMTGS+ PYL KLR YAG LPLVS DYGSTESWIGVNVDP LPPE+V+FAV+PTFSYF
Sbjct: 309 SSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLPPEDVSFAVIPTFSYF 368

Query: 401 EFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTG--------- 460
           EFIPL+R+  Q D     +    DF+E +P+PLSQVK+GQ+YELVLTTFTG         
Sbjct: 369 EFIPLYRRQNQSD-----ICIDGDFVEDKPVPLSQVKLGQEYELVLTTFTGLYRYRLGDV 428

Query: 461 -----FHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSH 520
                FH  TPKL+FI RRKL+LT+NIDKNTEKDLQ VV++ SQLL+  + AE+VDFTSH
Sbjct: 429 VEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQLLSRSTRAEVVDFTSH 488

Query: 521 ADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVER 580
           AD+  +PGHYVI+WE++G  +D  L  CC EMD AFVDYGYVVSRR N+IGPLELR+VER
Sbjct: 489 ADVIARPGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSRRMNSIGPLELRVVER 548

Query: 581 GSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 614
           G+F K+ E  +G    L+QFKTPRCTTN V+L ILN ST+K F S+AY
Sbjct: 549 GTFGKVAERCVGKCGGLNQFKTPRCTTNSVMLDILNDSTIKRFRSSAY 590

BLAST of Cp4.1LG16g04770 vs. TAIR 10
Match: AT2G46370.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 516.9 bits (1330), Expect = 2.2e-146
Identity = 265/599 (44.24%), Postives = 374/599 (62.44%), Query Frame = 0

Query: 36  MFEEVED-----MVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDD 95
           M E+VE      ++D F+ ++  A   Q +TL+EIL +N    YL       N     + 
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQ------NCGLNGNA 60

Query: 96  FALESLFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFT 155
              E  F S+VPL +  + EP+++R+ DGD+SP+LT  P+  +SLSSGT++GR K++PFT
Sbjct: 61  TDPEEAFKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFT 120

Query: 156 PHSAQTTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQ 215
               + TL +FR A A+R+R +P  + G+ L+FI+SSKQ  + GG+  GTATT+ Y +  
Sbjct: 121 DELMENTLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPN 180

Query: 216 FKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNEL 275
           FK       S +CSP EVI   D  Q+ YCHLL G+ F DQV++V + FA+ +V AF   
Sbjct: 181 FKAGMKSITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTF 240

Query: 276 EDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVP 335
           E  W +I  DI    L +RIT+P +R A+  +++PNP L   I   C  L   +W  L+P
Sbjct: 241 EQVWEEIVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIP 300

Query: 336 KLWPNCKYVYSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTF 395
            L+PN KYVY IMTGS++PY+ KLR YAG LPLVS DYGS+E WI  NV P L PE  TF
Sbjct: 301 ALFPNAKYVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATF 360

Query: 396 AVVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFT- 455
           AV+P   YFEF+P+       + G           E +P+ L+QVKIG++YE+V+T +  
Sbjct: 361 AVIPNLGYFEFLPV------SETGEG---------EEKPVGLTQVKIGEEYEVVITNYAG 420

Query: 456 -------------GFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSG 515
                        GF+N TP+L FICRR L+L++NIDKNTE+DLQL VE  ++ L+++  
Sbjct: 421 LYRYRLGDVVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK- 480

Query: 516 AELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIG 575
            E++DF+S+ D++  PGHY IFWE+ G   + VL  CC+ +D AF+D GYV SR+  TIG
Sbjct: 481 IEVIDFSSYIDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIG 540

Query: 576 PLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAY 614
            LELR+V +G+F KI EH++G G++  QFK PRC   +N  +L IL  + V ++ STA+
Sbjct: 541 ALELRVVAKGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

BLAST of Cp4.1LG16g04770 vs. TAIR 10
Match: AT2G46370.2 (Auxin-responsive GH3 family protein )

HSP 1 Score: 516.9 bits (1330), Expect = 2.2e-146
Identity = 265/599 (44.24%), Postives = 374/599 (62.44%), Query Frame = 0

Query: 36  MFEEVED-----MVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDD 95
           M E+VE      ++D F+ ++  A   Q +TL+EIL +N    YL       N     + 
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQ------NCGLNGNA 60

Query: 96  FALESLFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFT 155
              E  F S+VPL +  + EP+++R+ DGD+SP+LT  P+  +SLSSGT++GR K++PFT
Sbjct: 61  TDPEEAFKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFT 120

Query: 156 PHSAQTTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQ 215
               + TL +FR A A+R+R +P  + G+ L+FI+SSKQ  + GG+  GTATT+ Y +  
Sbjct: 121 DELMENTLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPN 180

Query: 216 FKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNEL 275
           FK       S +CSP EVI   D  Q+ YCHLL G+ F DQV++V + FA+ +V AF   
Sbjct: 181 FKAGMKSITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTF 240

Query: 276 EDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVP 335
           E  W +I  DI    L +RIT+P +R A+  +++PNP L   I   C  L   +W  L+P
Sbjct: 241 EQVWEEIVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIP 300

Query: 336 KLWPNCKYVYSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTF 395
            L+PN KYVY IMTGS++PY+ KLR YAG LPLVS DYGS+E WI  NV P L PE  TF
Sbjct: 301 ALFPNAKYVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATF 360

Query: 396 AVVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFT- 455
           AV+P   YFEF+P+       + G           E +P+ L+QVKIG++YE+V+T +  
Sbjct: 361 AVIPNLGYFEFLPV------SETGEG---------EEKPVGLTQVKIGEEYEVVITNYAG 420

Query: 456 -------------GFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSG 515
                        GF+N TP+L FICRR L+L++NIDKNTE+DLQL VE  ++ L+++  
Sbjct: 421 LYRYRLGDVVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK- 480

Query: 516 AELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIG 575
            E++DF+S+ D++  PGHY IFWE+ G   + VL  CC+ +D AF+D GYV SR+  TIG
Sbjct: 481 IEVIDFSSYIDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIG 540

Query: 576 PLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAY 614
            LELR+V +G+F KI EH++G G++  QFK PRC   +N  +L IL  + V ++ STA+
Sbjct: 541 ALELRVVAKGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

BLAST of Cp4.1LG16g04770 vs. TAIR 10
Match: AT2G46370.4 (Auxin-responsive GH3 family protein )

HSP 1 Score: 516.9 bits (1330), Expect = 2.2e-146
Identity = 265/599 (44.24%), Postives = 374/599 (62.44%), Query Frame = 0

Query: 36  MFEEVED-----MVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDD 95
           M E+VE      ++D F+ ++  A   Q +TL+EIL +N    YL       N     + 
Sbjct: 12  MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQ------NCGLNGNA 71

Query: 96  FALESLFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFT 155
              E  F S+VPL +  + EP+++R+ DGD+SP+LT  P+  +SLSSGT++GR K++PFT
Sbjct: 72  TDPEEAFKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFT 131

Query: 156 PHSAQTTLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQ 215
               + TL +FR A A+R+R +P  + G+ L+FI+SSKQ  + GG+  GTATT+ Y +  
Sbjct: 132 DELMENTLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPN 191

Query: 216 FKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNEL 275
           FK       S +CSP EVI   D  Q+ YCHLL G+ F DQV++V + FA+ +V AF   
Sbjct: 192 FKAGMKSITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTF 251

Query: 276 EDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVP 335
           E  W +I  DI    L +RIT+P +R A+  +++PNP L   I   C  L   +W  L+P
Sbjct: 252 EQVWEEIVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIP 311

Query: 336 KLWPNCKYVYSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTF 395
            L+PN KYVY IMTGS++PY+ KLR YAG LPLVS DYGS+E WI  NV P L PE  TF
Sbjct: 312 ALFPNAKYVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATF 371

Query: 396 AVVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFT- 455
           AV+P   YFEF+P+       + G           E +P+ L+QVKIG++YE+V+T +  
Sbjct: 372 AVIPNLGYFEFLPV------SETGEG---------EEKPVGLTQVKIGEEYEVVITNYAG 431

Query: 456 -------------GFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSG 515
                        GF+N TP+L FICRR L+L++NIDKNTE+DLQL VE  ++ L+++  
Sbjct: 432 LYRYRLGDVVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK- 491

Query: 516 AELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIG 575
            E++DF+S+ D++  PGHY IFWE+ G   + VL  CC+ +D AF+D GYV SR+  TIG
Sbjct: 492 IEVIDFSSYIDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIG 551

Query: 576 PLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAY 614
            LELR+V +G+F KI EH++G G++  QFK PRC   +N  +L IL  + V ++ STA+
Sbjct: 552 ALELRVVAKGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 586

BLAST of Cp4.1LG16g04770 vs. TAIR 10
Match: AT2G46370.3 (Auxin-responsive GH3 family protein )

HSP 1 Score: 483.4 bits (1243), Expect = 2.7e-136
Identity = 239/514 (46.50%), Postives = 332/514 (64.59%), Query Frame = 0

Query: 116 IADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLIFRIAAAYRSRVYPTR 175
           + DGD+SP+LT  P+  +SLSSGT++GR K++PFT    + TL +FR A A+R+R +P  
Sbjct: 1   MVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLFRTAFAFRNRDFPID 60

Query: 176 EGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYK 235
           + G+ L+FI+SSKQ  + GG+  GTATT+ Y +  FK       S +CSP EVI   D  
Sbjct: 61  DNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSPSCSPDEVIFSPDVH 120

Query: 236 QSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHDITHATLCSRITLPEI 295
           Q+ YCHLL G+ F DQV++V + FA+ +V AF   E  W +I  DI    L +RIT+P +
Sbjct: 121 QALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDIKDGVLSNRITVPSV 180

Query: 296 RKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCKYVYSIMTGSIQPYLKKLR 355
           R A+  +++PNP L   I   C  L   +W  L+P L+PN KYVY IMTGS++PY+ KLR
Sbjct: 181 RTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIPALFPNAKYVYGIMTGSMEPYVPKLR 240

Query: 356 RYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGS 415
            YAG LPLVS DYGS+E WI  NV P L PE  TFAV+P   YFEF+P+       + G 
Sbjct: 241 HYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEFLPV------SETGE 300

Query: 416 SSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFT--------------GFHNKTPKLNFI 475
                     E +P+ L+QVKIG++YE+V+T +               GF+N TP+L FI
Sbjct: 301 G---------EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNTPQLKFI 360

Query: 476 CRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEV 535
           CRR L+L++NIDKNTE+DLQL VE  ++ L+++   E++DF+S+ D++  PGHY IFWE+
Sbjct: 361 CRRNLILSINIDKNTERDLQLSVESAAKRLSEEK-IEVIDFSSYIDVSTDPGHYAIFWEI 420

Query: 536 KGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAA 595
            G   + VL  CC+ +D AF+D GYV SR+  TIG LELR+V +G+F KI EH++G G++
Sbjct: 421 SGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFLGLGSS 480

Query: 596 LSQFKTPRCT--TNQVLLTILNLSTVKAFHSTAY 614
             QFK PRC   +N  +L IL  + V ++ STA+
Sbjct: 481 AGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 496