Cp4.1LG16g03540 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG16g03540
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionamino acid transporter AVT3B-like
LocationCp4.1LG16: 5273073 .. 5274356 (-)
RNA-Seq ExpressionCp4.1LG16g03540
SyntenyCp4.1LG16g03540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTCCGATCTAGAAGCCAGTTCTTCATCCAGTCTCCTTCAATCTCCGGCGGTCAAAGAAGATACGCCTCTTCTCGGCAGCGCCAACCCTCTGTCTTCGCAGCCCAAGACATTTGCTAATGTTTTTATTGCCATTGTTGGGGCTGGTGTTCTTGGGCTTCCTTACGCTTTCAAACGTACTGGGTGGGTTATGAGCCTTCTAATGCTGTTTTCTGTTGCGTTCTTCACTTATTACTGTATGATGCTCCTTGTTTATACTCGTCGGAAAATTGAATCGGTTATCGGATTCTCGAAAATCAATTCGTTTGGGGATTTAGGTTATACTATTTGCGGTTCCCCTGGTAGGATTATTGTTGATTTGCTTATTATTTTGTCTCAAACTGGATTCTGTGTTGGTTATTTGATTTTCATTGGTAATACTATGGCTGATGTTTATAATTCCCCGACCGCTACGGATTTGAGTCCTAAGATCTTGGGATTAGCGCCCAAGGTTGTGTACGTTTGGGGGTGTCTCCCTTTCCAATTGGGTTTGAACTCGATTCCAACCCTGACCCATTTGGCTCCTTTGAGTATCTTTGCTGATGTTGTCGACCTTGGAGCTATGGTGGTTGTGATGATAAAAGACGTGTTGATTTACTTCAAACAGAGCCCATCTGTTGAGGCATTTGGGGGCTTTTCCGTGTTCTTTTACGGAATGGGTGTGGCTGTGTATGCCTTTGAAGGGATTGGAATGGTGTTGCCATTAGAATCCGAGACAAAGAACAAGGAGAAATTCGGGAGTGTCTTGGGGTTATCCATGGCGTTCATTACTGTTTTATACGGAGCATTTGGAACTCTTGGCTATTTCGCCTTCGGCGAAGATACCAAAGACATGATCACCGGTAACTTAGGGTCTGGATTAATTAGCACCATTGTTAAACTGGGTTTGTGTGTAAACCTGTTCTTCACGTTGCCATTGATGATGAACCCTGTTTATGAGATCGTGGAGAGACGATTCTGGGGAGGAAGATATAGCCTATGGTTAAGATGGATGCTGGTTTTCATGGTGAGTTTGGTGGCACTTTTGGTGCCGAATTTTGCTGATTTCTTGTCTCTGGTTGGAAGCGCTGTGTGCTGTGCATTGGCGTTTGTGTTGCCTGCTCTGTTCCATTTCTTGGTGTTCAAGCAGGAAATGGATGTGAAAGGATGGTGTGTGGACATTGGAATTCTGGTGTTGGGGGTTGTTCTTGGAGTTTCTGGGACATGGTCTGCTCTGGTGGTGATTTTCTCTGTTAAGGAATGA

mRNA sequence

ATGGGTTCCGATCTAGAAGCCAGTTCTTCATCCAGTCTCCTTCAATCTCCGGCGGTCAAAGAAGATACGCCTCTTCTCGGCAGCGCCAACCCTCTGTCTTCGCAGCCCAAGACATTTGCTAATGTTTTTATTGCCATTGTTGGGGCTGGTGTTCTTGGGCTTCCTTACGCTTTCAAACGTACTGGGTGGGTTATGAGCCTTCTAATGCTGTTTTCTGTTGCGTTCTTCACTTATTACTGTATGATGCTCCTTGTTTATACTCGTCGGAAAATTGAATCGGTTATCGGATTCTCGAAAATCAATTCGTTTGGGGATTTAGGTTATACTATTTGCGGTTCCCCTGGTAGGATTATTGTTGATTTGCTTATTATTTTGTCTCAAACTGGATTCTGTGTTGGTTATTTGATTTTCATTGGTAATACTATGGCTGATGTTTATAATTCCCCGACCGCTACGGATTTGAGTCCTAAGATCTTGGGATTAGCGCCCAAGGTTGTGTACGTTTGGGGGTGTCTCCCTTTCCAATTGGGTTTGAACTCGATTCCAACCCTGACCCATTTGGCTCCTTTGAGTATCTTTGCTGATGTTGTCGACCTTGGAGCTATGGTGGTTGTGATGATAAAAGACGTGTTGATTTACTTCAAACAGAGCCCATCTGTTGAGGCATTTGGGGGCTTTTCCGTGTTCTTTTACGGAATGGGTGTGGCTGTGTATGCCTTTGAAGGGATTGGAATGGTGTTGCCATTAGAATCCGAGACAAAGAACAAGGAGAAATTCGGGAGTGTCTTGGGGTTATCCATGGCGTTCATTACTGTTTTATACGGAGCATTTGGAACTCTTGGCTATTTCGCCTTCGGCGAAGATACCAAAGACATGATCACCGGTAACTTAGGGTCTGGATTAATTAGCACCATTGTTAAACTGGGTTTGTGTGTAAACCTGTTCTTCACGTTGCCATTGATGATGAACCCTGTTTATGAGATCGTGGAGAGACGATTCTGGGGAGGAAGATATAGCCTATGGTTAAGATGGATGCTGGTTTTCATGGTGAGTTTGGTGGCACTTTTGGTGCCGAATTTTGCTGATTTCTTGTCTCTGGTTGGAAGCGCTGTGTGCTGTGCATTGGCGTTTGTGTTGCCTGCTCTGTTCCATTTCTTGGTGTTCAAGCAGGAAATGGATGTGAAAGGATGGTGTGTGGACATTGGAATTCTGGTGTTGGGGGTTGTTCTTGGAGTTTCTGGGACATGGTCTGCTCTGGTGGTGATTTTCTCTGTTAAGGAATGA

Coding sequence (CDS)

ATGGGTTCCGATCTAGAAGCCAGTTCTTCATCCAGTCTCCTTCAATCTCCGGCGGTCAAAGAAGATACGCCTCTTCTCGGCAGCGCCAACCCTCTGTCTTCGCAGCCCAAGACATTTGCTAATGTTTTTATTGCCATTGTTGGGGCTGGTGTTCTTGGGCTTCCTTACGCTTTCAAACGTACTGGGTGGGTTATGAGCCTTCTAATGCTGTTTTCTGTTGCGTTCTTCACTTATTACTGTATGATGCTCCTTGTTTATACTCGTCGGAAAATTGAATCGGTTATCGGATTCTCGAAAATCAATTCGTTTGGGGATTTAGGTTATACTATTTGCGGTTCCCCTGGTAGGATTATTGTTGATTTGCTTATTATTTTGTCTCAAACTGGATTCTGTGTTGGTTATTTGATTTTCATTGGTAATACTATGGCTGATGTTTATAATTCCCCGACCGCTACGGATTTGAGTCCTAAGATCTTGGGATTAGCGCCCAAGGTTGTGTACGTTTGGGGGTGTCTCCCTTTCCAATTGGGTTTGAACTCGATTCCAACCCTGACCCATTTGGCTCCTTTGAGTATCTTTGCTGATGTTGTCGACCTTGGAGCTATGGTGGTTGTGATGATAAAAGACGTGTTGATTTACTTCAAACAGAGCCCATCTGTTGAGGCATTTGGGGGCTTTTCCGTGTTCTTTTACGGAATGGGTGTGGCTGTGTATGCCTTTGAAGGGATTGGAATGGTGTTGCCATTAGAATCCGAGACAAAGAACAAGGAGAAATTCGGGAGTGTCTTGGGGTTATCCATGGCGTTCATTACTGTTTTATACGGAGCATTTGGAACTCTTGGCTATTTCGCCTTCGGCGAAGATACCAAAGACATGATCACCGGTAACTTAGGGTCTGGATTAATTAGCACCATTGTTAAACTGGGTTTGTGTGTAAACCTGTTCTTCACGTTGCCATTGATGATGAACCCTGTTTATGAGATCGTGGAGAGACGATTCTGGGGAGGAAGATATAGCCTATGGTTAAGATGGATGCTGGTTTTCATGGTGAGTTTGGTGGCACTTTTGGTGCCGAATTTTGCTGATTTCTTGTCTCTGGTTGGAAGCGCTGTGTGCTGTGCATTGGCGTTTGTGTTGCCTGCTCTGTTCCATTTCTTGGTGTTCAAGCAGGAAATGGATGTGAAAGGATGGTGTGTGGACATTGGAATTCTGGTGTTGGGGGTTGTTCTTGGAGTTTCTGGGACATGGTCTGCTCTGGTGGTGATTTTCTCTGTTAAGGAATGA

Protein sequence

MGSDLEASSSSSLLQSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKRTGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVDLLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNSIPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAFEGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSGLISTIVKLGLCVNLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNFADFLSLVGSAVCCALAFVLPALFHFLVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALVVIFSVKE
Homology
BLAST of Cp4.1LG16g03540 vs. ExPASy Swiss-Prot
Match: Q9SVG0 (Amino acid transporter AVT3C OS=Arabidopsis thaliana OX=3702 GN=AVT3C PE=1 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 6.2e-161
Identity = 299/435 (68.74%), Postives = 350/435 (80.46%), Query Frame = 0

Query: 1   MGSDLEASSSSSLLQ-SPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFK 60
           MG   EASSSS  L+  P  +ED+PLLG   PLSSQ KTFANVFIA+VGAGVLGLPYAFK
Sbjct: 1   MGFQNEASSSSYTLKIPPPAREDSPLLGKGPPLSSQFKTFANVFIAVVGAGVLGLPYAFK 60

Query: 61  RTGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESV-IGFSKINSFGDLGYTICGSPGRII 120
           RTGW+M +L+L SV+  T++CMMLLVYTRRK++S   G SKI SFGDLG+ +CGS GRI+
Sbjct: 61  RTGWLMGVLLLVSVSVLTHHCMMLLVYTRRKLDSFNAGISKIGSFGDLGFAVCGSLGRIV 120

Query: 121 VDLLIILSQTGFCVGYLIFIGNTMADVYN--SPTA-----TDLSPKILGLAPKVVYVWGC 180
           VDL IILSQ GFCVGYLIFIG T+A++ +  SPT+     T L  + LG++ K +Y+WGC
Sbjct: 121 VDLFIILSQAGFCVGYLIFIGTTLANLSDPESPTSLRHQFTRLGSEFLGVSSKSLYIWGC 180

Query: 181 LPFQLGLNSIPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFY 240
            PFQLGLNSI TLTHLAPLSIFAD+VDLGAM VV+++D +I  KQ P V AFGG S+F Y
Sbjct: 181 FPFQLGLNSIKTLTHLAPLSIFADIVDLGAMAVVIVEDSMIILKQRPDVVAFGGMSLFLY 240

Query: 241 GMGVAVYAFEGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKD 300
           GMGVAVY+FEG+GMVLPLESE K+K+KFG VL L M FI+++Y AFG LGY AFGEDT D
Sbjct: 241 GMGVAVYSFEGVGMVLPLESEMKDKDKFGKVLALGMGFISLIYIAFGILGYLAFGEDTMD 300

Query: 301 MITGNLGSGLISTIVKLGLCVNLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVS 360
           +IT NLG+GL+ST+V+LGLC+NLFFT PLMMNPV+EIVERRF  G YS WLRW+LV  V+
Sbjct: 301 IITANLGAGLVSTVVQLGLCINLFFTFPLMMNPVFEIVERRFSRGMYSAWLRWVLVLAVT 360

Query: 361 LVALLVPNFADFLSLVGSAVCCALAFVLPALFHFLVFKQEMDVKGWCVDIGILVLGVVLG 420
           LVAL VPNFADFLSLVGS+ CC L FVLPALFH LVFK+EM    W  D  I+VLGVVL 
Sbjct: 361 LVALFVPNFADFLSLVGSSTCCVLGFVLPALFHLLVFKEEMGWLQWSSDTAIVVLGVVLA 420

Query: 421 VSGTWSALVVIFSVK 427
           VSGTWS+L  IFSVK
Sbjct: 421 VSGTWSSLSEIFSVK 435

BLAST of Cp4.1LG16g03540 vs. ExPASy Swiss-Prot
Match: F4ILY9 (Amino acid transporter AVT3B OS=Arabidopsis thaliana OX=3702 GN=AVT3B PE=2 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 3.1e-160
Identity = 286/406 (70.44%), Postives = 337/406 (83.00%), Query Frame = 0

Query: 20  KEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKRTGWVMSLLMLFSVAFFTYY 79
           +EDTPLLG   PLSS+ KTFANVFIAIVGAGVLGLPYAFKRTGW+M LL LFSVA    +
Sbjct: 10  REDTPLLGKGRPLSSKFKTFANVFIAIVGAGVLGLPYAFKRTGWLMGLLTLFSVAALINH 69

Query: 80  CMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVDLLIILSQTGFCVGYLIFIG 139
           CMMLLV+ RRK    +G S I SFGDLG+  CG+ GR +VD+LIILSQ GFCVGYLIFIG
Sbjct: 70  CMMLLVHIRRK----LGVSNIGSFGDLGFAACGNLGRFVVDILIILSQAGFCVGYLIFIG 129

Query: 140 NTMADVYNSPTATDLSP--KILGLAPKVVYVWGCLPFQLGLNSIPTLTHLAPLSIFADVV 199
           NT+A++     +T L     ++G++PK +Y+WGC PFQLGLNSI TLTHLAPLSIFADVV
Sbjct: 130 NTLANLSKPTKSTTLMSLRHLMGVSPKSLYIWGCFPFQLGLNSIKTLTHLAPLSIFADVV 189

Query: 200 DLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAFEGIGMVLPLESETKNKE 259
           DLGAM VV+++D+ I   Q P V AFGG SVFFYGMGVAVYAFEG+GMVLPLESETK+K+
Sbjct: 190 DLGAMAVVIVEDIKITVVQRPQVVAFGGMSVFFYGMGVAVYAFEGVGMVLPLESETKDKD 249

Query: 260 KFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSGLISTIVKLGLCVNLFFT 319
           KFG VL LSM FI V+YG+FG LGY AFG+DT D+IT NLG+G++S++V+LGLC+NLFFT
Sbjct: 250 KFGKVLALSMLFIAVMYGSFGVLGYMAFGDDTMDIITANLGAGVVSSLVQLGLCINLFFT 309

Query: 320 LPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNFADFLSLVGSAVCCALAF 379
            PLMMNPV+EIVERRFW G Y +WLRW+LV  V+LVALLVPNFADFLSLVGS+VCCAL F
Sbjct: 310 FPLMMNPVFEIVERRFWSGMYCVWLRWLLVLAVTLVALLVPNFADFLSLVGSSVCCALGF 369

Query: 380 VLPALFHFLVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALVVIF 424
           VLP+LFH +VFK EM+ K   +D+GIL+LGV+LGVSGTWS+L  IF
Sbjct: 370 VLPSLFHLMVFKDEMEWKQRALDVGILLLGVILGVSGTWSSLTEIF 411

BLAST of Cp4.1LG16g03540 vs. ExPASy Swiss-Prot
Match: Q9FKY3 (Amino acid transporter AVT3A OS=Arabidopsis thaliana OX=3702 GN=AVT3A PE=1 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 1.8e-144
Identity = 263/432 (60.88%), Postives = 333/432 (77.08%), Query Frame = 0

Query: 1   MGSDLEASSSSSLLQSPAVK------EDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGL 60
           M  D EA SSS  L S +        EDTPLLG    LSSQPKTFANVFIAIVGAGVLGL
Sbjct: 1   MRYDQEAGSSSHSLPSGSSSHSLPPTEDTPLLG-PRTLSSQPKTFANVFIAIVGAGVLGL 60

Query: 61  PYAFKRTGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSP 120
           PY FK+TGW++ LL L  V+  T++CMMLLV+TRRK+ES+ GF+ I SFGDLG ++CG  
Sbjct: 61  PYTFKKTGWLLGLLTLLFVSSLTFFCMMLLVHTRRKLESLSGFNSITSFGDLGESVCGPA 120

Query: 121 GRIIVDLLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPF 180
           GR++VD++++LSQ+GFCV YLIF+  TMA++ +  T       ILGL    +Y+WGC PF
Sbjct: 121 GRLVVDVMLVLSQSGFCVSYLIFVATTMANLLSRGT-----EHILGLDAASIYLWGCFPF 180

Query: 181 QLGLNSIPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMG 240
           QLGLNSIP+LTHLAPLSIFAD+VD+ A +VVM++DV I+ K+ P +  FGG SVFFYG+G
Sbjct: 181 QLGLNSIPSLTHLAPLSIFADIVDVAATLVVMVQDVFIFLKRRPPLRVFGGVSVFFYGLG 240

Query: 241 VAVYAFEGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMIT 300
           VAVYAFEGIGMVLPLE E K K+KFG  LGL+M  I+++YGAFG LGY A+GE+TKD+IT
Sbjct: 241 VAVYAFEGIGMVLPLELEAKYKDKFGRALGLAMGLISIMYGAFGLLGYMAYGEETKDIIT 300

Query: 301 GNLGSGLISTIVKLGLCVNLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVA 360
            NLG+G++ST+V+LGL +NLFFT PLMM PVYE+VERR    RYS+W+RW  V +V+LVA
Sbjct: 301 TNLGTGVVSTLVQLGLAINLFFTFPLMMQPVYEVVERRLCSSRYSVWVRWATVLVVTLVA 360

Query: 361 LLVPNFADFLSLVGSAVCCALAFVLPALFHFLVFKQEMDVKGWCVDIGILVLGVVLGVSG 420
           LLVPNFADFLSLVGS+VC  L FVLP+LFH   FK E+ +    VD+ + ++GV++ ++G
Sbjct: 361 LLVPNFADFLSLVGSSVCVVLGFVLPSLFHLQAFKNELSITRIVVDVLVFLIGVMIAITG 420

Query: 421 TWSALVVIFSVK 427
           TW+A+  I + K
Sbjct: 421 TWTAVHEILTSK 426

BLAST of Cp4.1LG16g03540 vs. ExPASy Swiss-Prot
Match: Q9SF09 (Amino acid transporter ANT1 OS=Arabidopsis thaliana OX=3702 GN=ANT1 PE=1 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 3.0e-62
Identity = 156/440 (35.45%), Postives = 246/440 (55.91%), Query Frame = 0

Query: 4   DLEASSSSSLLQSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKRTGW 63
           DL A++  S L  P +K   P   +    +S  +T  N+ ++IVG GVLGLPYAF+  GW
Sbjct: 5   DLTATTGDSSL--PLIK-SPPSETTGGDRTSALQTLGNIIVSIVGTGVLGLPYAFRIAGW 64

Query: 64  VMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVDLLI 123
           +   L +  V F TYYCM+LL+  R K+ES  G  +  ++GDLG+   G+ GR + + LI
Sbjct: 65  LAGSLGVIIVGFATYYCMLLLIQCRDKLESEEGEEESKTYGDLGFKCMGTKGRYLTEFLI 124

Query: 124 ILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNSIPT 183
             +Q G  V YL+FIG  ++ +++S           GL+  V ++   +P ++GL+ I +
Sbjct: 125 FTAQCGGSVAYLVFIGRNLSSIFSS----------YGLS-MVSFILILVPIEVGLSWITS 184

Query: 184 LTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGM----GVAVYA 243
           L+ L+P SIFAD+ ++ AM  V+ ++V +  +   S       S    G+    GVAV+ 
Sbjct: 185 LSALSPFSIFADICNIIAMCFVVKENVEMVIEGDFSFSDRTAISSTIGGLPFAGGVAVFC 244

Query: 244 FEGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGS 303
           FEG  M L LES  + +E F  +L   +A IT +Y  FG  GY A+G+ TKD+IT NL +
Sbjct: 245 FEGFAMTLALESSMREREAFPKLLAKVLAGITFVYVLFGFCGYMAYGDQTKDIITLNLPN 304

Query: 304 GLISTIVKLGLCVNLFFTLPLMMNPVYEIVERRF----WGGRYS---------------L 363
              +  V++GLCV L FT P+M++P+ EI+E++     W  ++                 
Sbjct: 305 NWSAIAVQIGLCVGLTFTFPIMVHPLNEIIEQKLKRIDWLQKHHNGYSNETGSVSKFAIF 364

Query: 364 WLRWMLVFMVSLVALLVPNFADFLSLVGSAVCCALAFVLPALFHFLVFKQEMDVKGWCVD 421
             R +LV  ++ +A LVP F  F SLVGS +C  ++FVLPA +H  +    ++V    +D
Sbjct: 365 TTRTLLVVGLAAIASLVPGFGTFASLVGSTLCALISFVLPASYHLTLLGPSLNVWNKSID 424

BLAST of Cp4.1LG16g03540 vs. ExPASy Swiss-Prot
Match: Q495N2 (Proton-coupled amino acid transporter 3 OS=Homo sapiens OX=9606 GN=SLC36A3 PE=2 SV=2)

HSP 1 Score: 154.8 bits (390), Expect = 2.1e-36
Identity = 131/445 (29.44%), Postives = 221/445 (49.66%), Query Frame = 0

Query: 6   EASSSSSLLQSPAVKEDTPLLGSANPLS----SQPKTFANVFIAIVGAGVLGLPYAFKRT 65
           E +S  +  QSP+    +    + +P      S  +T  ++    +G G+LGLP A K  
Sbjct: 11  ELNSLDNGPQSPSESSSSITSENVHPAGEAGLSMMQTLIHLLKCNIGTGLLGLPLAIKNA 70

Query: 66  GWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGD---LGYTICGSP---- 125
           G ++  + L ++   T +CM++L+   + +   +  + +N +G+    G   C +     
Sbjct: 71  GLLVGPVSLLAIGVLTVHCMVILLNCAQHLSQRLQKTFVN-YGEATMYGLETCPNTWLRA 130

Query: 126 ----GRIIVDLLIILSQTGFCVGYLIFIGNTMADVYNSP--TATDLSPK-ILGLAPKV-- 185
               GR  V  L++++Q GFC  Y +F+ + +  +      T+    P+ IL L P +  
Sbjct: 131 HAVWGRYTVSFLLVITQLGFCSVYFMFMADNLQQMVEKAHVTSNICQPREILTLTPILDI 190

Query: 186 -VYVWGCLPFQLGLNSIPTLTHLAPLSIFADVVDLGAMVVVM--IKDVLIYFKQSPSVEA 245
             Y+   LPF + L  I  L  L+  S  A++  LG+M ++   I + + Y    P +  
Sbjct: 191 RFYMLIILPFLILLVFIQNLKVLSVFSTLANITTLGSMALIFEYIMEGIPYPSNLPLMAN 250

Query: 246 FGGFSVFFYGMGVAVYAFEGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGY 305
           +  F +FF   G A++ FEG+GMVLPL+++ K+ ++F  VL L M+ + +LY   GTLGY
Sbjct: 251 WKTFLLFF---GTAIFTFEGVGMVLPLKNQMKHPQQFSFVLYLGMSIVIILYILLGTLGY 310

Query: 306 FAFGEDTKDMITGNLGSGLISTIVKLGLCVNLFFTLPLMMNPVYEIV-------ERRFWG 365
             FG DT+  IT NL +  +   VKL   + +FFT  L  +   EI+           W 
Sbjct: 311 MKFGSDTQASITLNLPNCWLYQSVKLMYSIGIFFTYALQFHVPAEIIIPFAISQVSESWA 370

Query: 366 GRYSLWLRWMLVFMVSLVALLVPNFADFLSLVGSAVCCALAFVLPALFHFLVF-KQEMDV 420
               L +R  LV +  + A+L+P     +SLVGS    ALA ++PAL   ++F  ++M  
Sbjct: 371 LFVDLSVRSALVCLTCVSAILIPRLDLVISLVGSVSSSALALIIPALLEIVIFYSEDMSC 430

BLAST of Cp4.1LG16g03540 vs. NCBI nr
Match: XP_023512173.1 (amino acid transporter AVT3B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 827 bits (2137), Expect = 5.07e-302
Identity = 427/427 (100.00%), Postives = 427/427 (100.00%), Query Frame = 0

Query: 1   MGSDLEASSSSSLLQSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKR 60
           MGSDLEASSSSSLLQSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKR
Sbjct: 1   MGSDLEASSSSSLLQSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKR 60

Query: 61  TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVD 120
           TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVD
Sbjct: 61  TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVD 120

Query: 121 LLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS 180
           LLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS
Sbjct: 121 LLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS 180

Query: 181 IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF 240
           IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF
Sbjct: 181 IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF 240

Query: 241 EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG 300
           EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG
Sbjct: 241 EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG 300

Query: 301 LISTIVKLGLCVNLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF 360
           LISTIVKLGLCVNLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF
Sbjct: 301 LISTIVKLGLCVNLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF 360

Query: 361 ADFLSLVGSAVCCALAFVLPALFHFLVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALV 420
           ADFLSLVGSAVCCALAFVLPALFHFLVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALV
Sbjct: 361 ADFLSLVGSAVCCALAFVLPALFHFLVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALV 420

Query: 421 VIFSVKE 427
           VIFSVKE
Sbjct: 421 VIFSVKE 427

BLAST of Cp4.1LG16g03540 vs. NCBI nr
Match: KAG6570973.1 (Amino acid transporter AVT3B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 825 bits (2131), Expect = 4.16e-301
Identity = 424/427 (99.30%), Postives = 427/427 (100.00%), Query Frame = 0

Query: 1   MGSDLEASSSSSLLQSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKR 60
           MGSDLEASSSSSLLQSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKR
Sbjct: 1   MGSDLEASSSSSLLQSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKR 60

Query: 61  TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVD 120
           TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVD
Sbjct: 61  TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVD 120

Query: 121 LLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS 180
           LLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS
Sbjct: 121 LLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS 180

Query: 181 IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF 240
           IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF
Sbjct: 181 IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF 240

Query: 241 EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG 300
           EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG
Sbjct: 241 EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG 300

Query: 301 LISTIVKLGLCVNLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF 360
           LISTIVKLGLC+NLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF
Sbjct: 301 LISTIVKLGLCINLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF 360

Query: 361 ADFLSLVGSAVCCALAFVLPALFHFLVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALV 420
           ADFLSLVGSAVCCALAFVLPALFHF+VFKQEMDVKGWCVDIGILVLGVVLGVSGTWSAL+
Sbjct: 361 ADFLSLVGSAVCCALAFVLPALFHFMVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALL 420

Query: 421 VIFSVKE 427
           VIFSVKE
Sbjct: 421 VIFSVKE 427

BLAST of Cp4.1LG16g03540 vs. NCBI nr
Match: XP_022986978.1 (amino acid transporter AVT3B-like [Cucurbita maxima])

HSP 1 Score: 824 bits (2128), Expect = 1.19e-300
Identity = 423/427 (99.06%), Postives = 426/427 (99.77%), Query Frame = 0

Query: 1   MGSDLEASSSSSLLQSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKR 60
           MGSDLEASSSSSL QSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKR
Sbjct: 1   MGSDLEASSSSSLFQSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKR 60

Query: 61  TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVD 120
           TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESV+GFSKINSFGDLGYTICGSPGRIIVD
Sbjct: 61  TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVVGFSKINSFGDLGYTICGSPGRIIVD 120

Query: 121 LLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS 180
           LLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS
Sbjct: 121 LLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS 180

Query: 181 IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF 240
           IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF
Sbjct: 181 IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF 240

Query: 241 EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG 300
           EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG
Sbjct: 241 EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG 300

Query: 301 LISTIVKLGLCVNLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF 360
           LISTIVKLGLC+NLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF
Sbjct: 301 LISTIVKLGLCINLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF 360

Query: 361 ADFLSLVGSAVCCALAFVLPALFHFLVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALV 420
           ADFLSLVGSAVCCALAFVLP+LFHFLVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALV
Sbjct: 361 ADFLSLVGSAVCCALAFVLPSLFHFLVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALV 420

Query: 421 VIFSVKE 427
           VIFSVKE
Sbjct: 421 VIFSVKE 427

BLAST of Cp4.1LG16g03540 vs. NCBI nr
Match: KAG7010811.1 (Amino acid transporter AVT3B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 823 bits (2125), Expect = 3.42e-300
Identity = 423/427 (99.06%), Postives = 426/427 (99.77%), Query Frame = 0

Query: 1   MGSDLEASSSSSLLQSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKR 60
           MGSDLEASSSSSLLQSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKR
Sbjct: 1   MGSDLEASSSSSLLQSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKR 60

Query: 61  TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVD 120
           TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTIC SPGRIIVD
Sbjct: 61  TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICSSPGRIIVD 120

Query: 121 LLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS 180
           LLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS
Sbjct: 121 LLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS 180

Query: 181 IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF 240
           IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF
Sbjct: 181 IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF 240

Query: 241 EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG 300
           EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG
Sbjct: 241 EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG 300

Query: 301 LISTIVKLGLCVNLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF 360
           LISTIVKLGLC+NLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF
Sbjct: 301 LISTIVKLGLCINLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF 360

Query: 361 ADFLSLVGSAVCCALAFVLPALFHFLVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALV 420
           ADFLSLVGSAVCCALAFVLPALFHF+VFKQEMDVKGWCVDIGILVLGVVLGVSGTWSAL+
Sbjct: 361 ADFLSLVGSAVCCALAFVLPALFHFMVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALL 420

Query: 421 VIFSVKE 427
           VIFSVKE
Sbjct: 421 VIFSVKE 427

BLAST of Cp4.1LG16g03540 vs. NCBI nr
Match: XP_022943445.1 (amino acid transporter AVT3B-like [Cucurbita moschata])

HSP 1 Score: 822 bits (2123), Expect = 6.90e-300
Identity = 423/427 (99.06%), Postives = 425/427 (99.53%), Query Frame = 0

Query: 1   MGSDLEASSSSSLLQSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKR 60
           MGSDLEASSSSSLLQSPAVKEDTPLLGS NPLSSQPKTFANVFIAIVGAGVLGLPYAFKR
Sbjct: 1   MGSDLEASSSSSLLQSPAVKEDTPLLGSDNPLSSQPKTFANVFIAIVGAGVLGLPYAFKR 60

Query: 61  TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVD 120
           TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVD
Sbjct: 61  TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVD 120

Query: 121 LLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS 180
           LLI LSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS
Sbjct: 121 LLITLSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS 180

Query: 181 IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF 240
           IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF
Sbjct: 181 IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF 240

Query: 241 EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG 300
           EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG
Sbjct: 241 EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG 300

Query: 301 LISTIVKLGLCVNLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF 360
           LISTIVKLGLC+NLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF
Sbjct: 301 LISTIVKLGLCINLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF 360

Query: 361 ADFLSLVGSAVCCALAFVLPALFHFLVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALV 420
           ADFLSLVGSAVCCALAFVLPALFHF+VFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALV
Sbjct: 361 ADFLSLVGSAVCCALAFVLPALFHFMVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALV 420

Query: 421 VIFSVKE 427
           VIFSVKE
Sbjct: 421 VIFSVKE 427

BLAST of Cp4.1LG16g03540 vs. ExPASy TrEMBL
Match: A0A6J1JI45 (amino acid transporter AVT3B-like OS=Cucurbita maxima OX=3661 GN=LOC111484568 PE=3 SV=1)

HSP 1 Score: 824 bits (2128), Expect = 5.77e-301
Identity = 423/427 (99.06%), Postives = 426/427 (99.77%), Query Frame = 0

Query: 1   MGSDLEASSSSSLLQSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKR 60
           MGSDLEASSSSSL QSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKR
Sbjct: 1   MGSDLEASSSSSLFQSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKR 60

Query: 61  TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVD 120
           TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESV+GFSKINSFGDLGYTICGSPGRIIVD
Sbjct: 61  TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVVGFSKINSFGDLGYTICGSPGRIIVD 120

Query: 121 LLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS 180
           LLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS
Sbjct: 121 LLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS 180

Query: 181 IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF 240
           IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF
Sbjct: 181 IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF 240

Query: 241 EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG 300
           EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG
Sbjct: 241 EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG 300

Query: 301 LISTIVKLGLCVNLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF 360
           LISTIVKLGLC+NLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF
Sbjct: 301 LISTIVKLGLCINLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF 360

Query: 361 ADFLSLVGSAVCCALAFVLPALFHFLVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALV 420
           ADFLSLVGSAVCCALAFVLP+LFHFLVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALV
Sbjct: 361 ADFLSLVGSAVCCALAFVLPSLFHFLVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALV 420

Query: 421 VIFSVKE 427
           VIFSVKE
Sbjct: 421 VIFSVKE 427

BLAST of Cp4.1LG16g03540 vs. ExPASy TrEMBL
Match: A0A6J1FXT7 (amino acid transporter AVT3B-like OS=Cucurbita moschata OX=3662 GN=LOC111448211 PE=3 SV=1)

HSP 1 Score: 822 bits (2123), Expect = 3.34e-300
Identity = 423/427 (99.06%), Postives = 425/427 (99.53%), Query Frame = 0

Query: 1   MGSDLEASSSSSLLQSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKR 60
           MGSDLEASSSSSLLQSPAVKEDTPLLGS NPLSSQPKTFANVFIAIVGAGVLGLPYAFKR
Sbjct: 1   MGSDLEASSSSSLLQSPAVKEDTPLLGSDNPLSSQPKTFANVFIAIVGAGVLGLPYAFKR 60

Query: 61  TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVD 120
           TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVD
Sbjct: 61  TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVD 120

Query: 121 LLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS 180
           LLI LSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS
Sbjct: 121 LLITLSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS 180

Query: 181 IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF 240
           IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF
Sbjct: 181 IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF 240

Query: 241 EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG 300
           EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG
Sbjct: 241 EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG 300

Query: 301 LISTIVKLGLCVNLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF 360
           LISTIVKLGLC+NLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF
Sbjct: 301 LISTIVKLGLCINLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF 360

Query: 361 ADFLSLVGSAVCCALAFVLPALFHFLVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALV 420
           ADFLSLVGSAVCCALAFVLPALFHF+VFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALV
Sbjct: 361 ADFLSLVGSAVCCALAFVLPALFHFMVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALV 420

Query: 421 VIFSVKE 427
           VIFSVKE
Sbjct: 421 VIFSVKE 427

BLAST of Cp4.1LG16g03540 vs. ExPASy TrEMBL
Match: A0A0A0KFY3 (Aa_trans domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G118340 PE=3 SV=1)

HSP 1 Score: 760 bits (1962), Expect = 1.14e-275
Identity = 385/426 (90.38%), Postives = 406/426 (95.31%), Query Frame = 0

Query: 1   MGSDLEASSSSSLLQSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKR 60
           MG D EASSSS+ L+S   +EDTPLLG+  PLSSQPKTFANVFIAIVGAGVLGLPYAFKR
Sbjct: 1   MGFDREASSSSNRLRSSVAREDTPLLGTPIPLSSQPKTFANVFIAIVGAGVLGLPYAFKR 60

Query: 61  TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVD 120
           TGWVMSLLMLFSV+F TYYCMMLLVYTRRKIES+IGFSKINSFGDLGYTICGSPGR+IVD
Sbjct: 61  TGWVMSLLMLFSVSFLTYYCMMLLVYTRRKIESLIGFSKINSFGDLGYTICGSPGRLIVD 120

Query: 121 LLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS 180
            LIILSQTGFCVGYLIFIGNTMADV+NSPT  DL+PKILGL PKVVYVWGC PFQLGLNS
Sbjct: 121 FLIILSQTGFCVGYLIFIGNTMADVFNSPTVMDLNPKILGLVPKVVYVWGCFPFQLGLNS 180

Query: 181 IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF 240
           I TLTHLAPLSIFAD+VDLGAMVVVM+KDVLI FKQSPSVEAFGGFSVFFYGMGVAVYAF
Sbjct: 181 IQTLTHLAPLSIFADIVDLGAMVVVMVKDVLIIFKQSPSVEAFGGFSVFFYGMGVAVYAF 240

Query: 241 EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG 300
           EGIGMVLPLESETK+KEKFG VLGLSMAFITVLYGAFGTLGYFAFG+DTKDMITGNLGSG
Sbjct: 241 EGIGMVLPLESETKDKEKFGRVLGLSMAFITVLYGAFGTLGYFAFGKDTKDMITGNLGSG 300

Query: 301 LISTIVKLGLCVNLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF 360
            IST+VKLGLC+NLFFTLPLMMNPVYEIVERRFWGGRY LWLRW+LVF+VSLVALLVPNF
Sbjct: 301 FISTVVKLGLCINLFFTLPLMMNPVYEIVERRFWGGRYCLWLRWLLVFLVSLVALLVPNF 360

Query: 361 ADFLSLVGSAVCCALAFVLPALFHFLVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALV 420
           ADFLSLVGSAVCCALAFVLPALFHFLVFKQE+D+KGWC+DIGILVLG+VLGVSGTWSALV
Sbjct: 361 ADFLSLVGSAVCCALAFVLPALFHFLVFKQELDIKGWCLDIGILVLGLVLGVSGTWSALV 420

Query: 421 VIFSVK 426
            IFSVK
Sbjct: 421 EIFSVK 426

BLAST of Cp4.1LG16g03540 vs. ExPASy TrEMBL
Match: A0A5A7U0U9 (Amino acid transporter ANTL1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold133G00940 PE=3 SV=1)

HSP 1 Score: 756 bits (1952), Expect = 3.80e-274
Identity = 384/426 (90.14%), Postives = 404/426 (94.84%), Query Frame = 0

Query: 1   MGSDLEASSSSSLLQSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKR 60
           MG D EASSSS+ L+S   +EDTPLLG+  PLSSQPKTFANVFIAIVGAGVLGLPYAFKR
Sbjct: 1   MGFDREASSSSNRLRSTVAREDTPLLGAPLPLSSQPKTFANVFIAIVGAGVLGLPYAFKR 60

Query: 61  TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVD 120
           TGWVMSLLMLFSV+F TYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGR IVD
Sbjct: 61  TGWVMSLLMLFSVSFLTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRFIVD 120

Query: 121 LLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS 180
           LLIILSQTGFCVGYLIFIGNTMADV+NSPT  DL+PKILGLAPKV YVWGC PFQLGLNS
Sbjct: 121 LLIILSQTGFCVGYLIFIGNTMADVFNSPTVMDLNPKILGLAPKVAYVWGCFPFQLGLNS 180

Query: 181 IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF 240
           I TLTHLAPLSIFAD+VDLGAMVVVM+KDVLI F+QSPSVEAFGGFSVFFYGMGVAVYAF
Sbjct: 181 IQTLTHLAPLSIFADIVDLGAMVVVMVKDVLIIFEQSPSVEAFGGFSVFFYGMGVAVYAF 240

Query: 241 EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG 300
           EGIGMVLPLESETK+KEKFG VLGLSMAFITVLYGAFGTLGYFAFG+DTKDMIT NLG G
Sbjct: 241 EGIGMVLPLESETKDKEKFGRVLGLSMAFITVLYGAFGTLGYFAFGKDTKDMITSNLGPG 300

Query: 301 LISTIVKLGLCVNLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF 360
            IST+VKLGLC+NLFFTLPLMMNPVYEIVERRFWGGRY LWLRW+LVF+VSLVALLVPNF
Sbjct: 301 FISTVVKLGLCINLFFTLPLMMNPVYEIVERRFWGGRYCLWLRWLLVFLVSLVALLVPNF 360

Query: 361 ADFLSLVGSAVCCALAFVLPALFHFLVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALV 420
           ADFLSLVGSAVCCALAFVLPALFHFLVFKQE+D+KGWC+DIGILVLG+VLGVSGTWSALV
Sbjct: 361 ADFLSLVGSAVCCALAFVLPALFHFLVFKQELDIKGWCLDIGILVLGLVLGVSGTWSALV 420

Query: 421 VIFSVK 426
            IFSVK
Sbjct: 421 EIFSVK 426

BLAST of Cp4.1LG16g03540 vs. ExPASy TrEMBL
Match: A0A5D3CW42 (Amino acid transporter ANTL1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1275G00330 PE=3 SV=1)

HSP 1 Score: 753 bits (1944), Expect = 6.29e-273
Identity = 383/426 (89.91%), Postives = 403/426 (94.60%), Query Frame = 0

Query: 1   MGSDLEASSSSSLLQSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKR 60
           MG D EASSSS+ L+S   +EDTPLLG+  PLSSQPKTFANVFIAIVGAGVLGLPYAFKR
Sbjct: 1   MGFDREASSSSNRLRSTVAREDTPLLGAPLPLSSQPKTFANVFIAIVGAGVLGLPYAFKR 60

Query: 61  TGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVD 120
           TGWVMSLLMLFSV+F TYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGR IVD
Sbjct: 61  TGWVMSLLMLFSVSFLTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRFIVD 120

Query: 121 LLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNS 180
           LLIILSQTGFCVGYLIFIGNTMADV+NSPT  DL+PKILGLAPKV YVWGC PFQLGLNS
Sbjct: 121 LLIILSQTGFCVGYLIFIGNTMADVFNSPTVMDLNPKILGLAPKVAYVWGCFPFQLGLNS 180

Query: 181 IPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF 240
           I TLTHLAPLSIFAD+VDLGAMVVVM+KDVLI F+QS SVEAFGGFSVFFYGMGVAVYAF
Sbjct: 181 IQTLTHLAPLSIFADIVDLGAMVVVMVKDVLIIFEQSQSVEAFGGFSVFFYGMGVAVYAF 240

Query: 241 EGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSG 300
           EGIGMVLPLESETK+KEKFG VLGLSMAFITVLYGAFGTLGYFAFG+DTKDMIT NLG G
Sbjct: 241 EGIGMVLPLESETKDKEKFGRVLGLSMAFITVLYGAFGTLGYFAFGKDTKDMITSNLGPG 300

Query: 301 LISTIVKLGLCVNLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNF 360
            IST+VKLGLC+NLFFTLPLMMNPVYEIVERRFWGGRY LWLRW+LVF+VSLVALLVPNF
Sbjct: 301 FISTVVKLGLCINLFFTLPLMMNPVYEIVERRFWGGRYCLWLRWLLVFLVSLVALLVPNF 360

Query: 361 ADFLSLVGSAVCCALAFVLPALFHFLVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALV 420
           ADFLSLVGSAVCCALAFVLPALFHFLVFKQE+D+KGWC+DIGILVLG+VLGVSGTWSALV
Sbjct: 361 ADFLSLVGSAVCCALAFVLPALFHFLVFKQELDIKGWCLDIGILVLGLVLGVSGTWSALV 420

Query: 421 VIFSVK 426
            IFSVK
Sbjct: 421 EIFSVK 426

BLAST of Cp4.1LG16g03540 vs. TAIR 10
Match: AT4G38250.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 568.5 bits (1464), Expect = 4.4e-162
Identity = 299/435 (68.74%), Postives = 350/435 (80.46%), Query Frame = 0

Query: 1   MGSDLEASSSSSLLQ-SPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFK 60
           MG   EASSSS  L+  P  +ED+PLLG   PLSSQ KTFANVFIA+VGAGVLGLPYAFK
Sbjct: 1   MGFQNEASSSSYTLKIPPPAREDSPLLGKGPPLSSQFKTFANVFIAVVGAGVLGLPYAFK 60

Query: 61  RTGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESV-IGFSKINSFGDLGYTICGSPGRII 120
           RTGW+M +L+L SV+  T++CMMLLVYTRRK++S   G SKI SFGDLG+ +CGS GRI+
Sbjct: 61  RTGWLMGVLLLVSVSVLTHHCMMLLVYTRRKLDSFNAGISKIGSFGDLGFAVCGSLGRIV 120

Query: 121 VDLLIILSQTGFCVGYLIFIGNTMADVYN--SPTA-----TDLSPKILGLAPKVVYVWGC 180
           VDL IILSQ GFCVGYLIFIG T+A++ +  SPT+     T L  + LG++ K +Y+WGC
Sbjct: 121 VDLFIILSQAGFCVGYLIFIGTTLANLSDPESPTSLRHQFTRLGSEFLGVSSKSLYIWGC 180

Query: 181 LPFQLGLNSIPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFY 240
            PFQLGLNSI TLTHLAPLSIFAD+VDLGAM VV+++D +I  KQ P V AFGG S+F Y
Sbjct: 181 FPFQLGLNSIKTLTHLAPLSIFADIVDLGAMAVVIVEDSMIILKQRPDVVAFGGMSLFLY 240

Query: 241 GMGVAVYAFEGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKD 300
           GMGVAVY+FEG+GMVLPLESE K+K+KFG VL L M FI+++Y AFG LGY AFGEDT D
Sbjct: 241 GMGVAVYSFEGVGMVLPLESEMKDKDKFGKVLALGMGFISLIYIAFGILGYLAFGEDTMD 300

Query: 301 MITGNLGSGLISTIVKLGLCVNLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVS 360
           +IT NLG+GL+ST+V+LGLC+NLFFT PLMMNPV+EIVERRF  G YS WLRW+LV  V+
Sbjct: 301 IITANLGAGLVSTVVQLGLCINLFFTFPLMMNPVFEIVERRFSRGMYSAWLRWVLVLAVT 360

Query: 361 LVALLVPNFADFLSLVGSAVCCALAFVLPALFHFLVFKQEMDVKGWCVDIGILVLGVVLG 420
           LVAL VPNFADFLSLVGS+ CC L FVLPALFH LVFK+EM    W  D  I+VLGVVL 
Sbjct: 361 LVALFVPNFADFLSLVGSSTCCVLGFVLPALFHLLVFKEEMGWLQWSSDTAIVVLGVVLA 420

Query: 421 VSGTWSALVVIFSVK 427
           VSGTWS+L  IFSVK
Sbjct: 421 VSGTWSSLSEIFSVK 435

BLAST of Cp4.1LG16g03540 vs. TAIR 10
Match: AT2G42005.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 566.2 bits (1458), Expect = 2.2e-161
Identity = 286/406 (70.44%), Postives = 337/406 (83.00%), Query Frame = 0

Query: 20  KEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKRTGWVMSLLMLFSVAFFTYY 79
           +EDTPLLG   PLSS+ KTFANVFIAIVGAGVLGLPYAFKRTGW+M LL LFSVA    +
Sbjct: 10  REDTPLLGKGRPLSSKFKTFANVFIAIVGAGVLGLPYAFKRTGWLMGLLTLFSVAALINH 69

Query: 80  CMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVDLLIILSQTGFCVGYLIFIG 139
           CMMLLV+ RRK    +G S I SFGDLG+  CG+ GR +VD+LIILSQ GFCVGYLIFIG
Sbjct: 70  CMMLLVHIRRK----LGVSNIGSFGDLGFAACGNLGRFVVDILIILSQAGFCVGYLIFIG 129

Query: 140 NTMADVYNSPTATDLSP--KILGLAPKVVYVWGCLPFQLGLNSIPTLTHLAPLSIFADVV 199
           NT+A++     +T L     ++G++PK +Y+WGC PFQLGLNSI TLTHLAPLSIFADVV
Sbjct: 130 NTLANLSKPTKSTTLMSLRHLMGVSPKSLYIWGCFPFQLGLNSIKTLTHLAPLSIFADVV 189

Query: 200 DLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAFEGIGMVLPLESETKNKE 259
           DLGAM VV+++D+ I   Q P V AFGG SVFFYGMGVAVYAFEG+GMVLPLESETK+K+
Sbjct: 190 DLGAMAVVIVEDIKITVVQRPQVVAFGGMSVFFYGMGVAVYAFEGVGMVLPLESETKDKD 249

Query: 260 KFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSGLISTIVKLGLCVNLFFT 319
           KFG VL LSM FI V+YG+FG LGY AFG+DT D+IT NLG+G++S++V+LGLC+NLFFT
Sbjct: 250 KFGKVLALSMLFIAVMYGSFGVLGYMAFGDDTMDIITANLGAGVVSSLVQLGLCINLFFT 309

Query: 320 LPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVALLVPNFADFLSLVGSAVCCALAF 379
            PLMMNPV+EIVERRFW G Y +WLRW+LV  V+LVALLVPNFADFLSLVGS+VCCAL F
Sbjct: 310 FPLMMNPVFEIVERRFWSGMYCVWLRWLLVLAVTLVALLVPNFADFLSLVGSSVCCALGF 369

Query: 380 VLPALFHFLVFKQEMDVKGWCVDIGILVLGVVLGVSGTWSALVVIF 424
           VLP+LFH +VFK EM+ K   +D+GIL+LGV+LGVSGTWS+L  IF
Sbjct: 370 VLPSLFHLMVFKDEMEWKQRALDVGILLLGVILGVSGTWSSLTEIF 411

BLAST of Cp4.1LG16g03540 vs. TAIR 10
Match: AT5G65990.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 513.8 bits (1322), Expect = 1.3e-145
Identity = 263/432 (60.88%), Postives = 333/432 (77.08%), Query Frame = 0

Query: 1   MGSDLEASSSSSLLQSPAVK------EDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGL 60
           M  D EA SSS  L S +        EDTPLLG    LSSQPKTFANVFIAIVGAGVLGL
Sbjct: 1   MRYDQEAGSSSHSLPSGSSSHSLPPTEDTPLLG-PRTLSSQPKTFANVFIAIVGAGVLGL 60

Query: 61  PYAFKRTGWVMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSP 120
           PY FK+TGW++ LL L  V+  T++CMMLLV+TRRK+ES+ GF+ I SFGDLG ++CG  
Sbjct: 61  PYTFKKTGWLLGLLTLLFVSSLTFFCMMLLVHTRRKLESLSGFNSITSFGDLGESVCGPA 120

Query: 121 GRIIVDLLIILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPF 180
           GR++VD++++LSQ+GFCV YLIF+  TMA++ +  T       ILGL    +Y+WGC PF
Sbjct: 121 GRLVVDVMLVLSQSGFCVSYLIFVATTMANLLSRGT-----EHILGLDAASIYLWGCFPF 180

Query: 181 QLGLNSIPTLTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMG 240
           QLGLNSIP+LTHLAPLSIFAD+VD+ A +VVM++DV I+ K+ P +  FGG SVFFYG+G
Sbjct: 181 QLGLNSIPSLTHLAPLSIFADIVDVAATLVVMVQDVFIFLKRRPPLRVFGGVSVFFYGLG 240

Query: 241 VAVYAFEGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMIT 300
           VAVYAFEGIGMVLPLE E K K+KFG  LGL+M  I+++YGAFG LGY A+GE+TKD+IT
Sbjct: 241 VAVYAFEGIGMVLPLELEAKYKDKFGRALGLAMGLISIMYGAFGLLGYMAYGEETKDIIT 300

Query: 301 GNLGSGLISTIVKLGLCVNLFFTLPLMMNPVYEIVERRFWGGRYSLWLRWMLVFMVSLVA 360
            NLG+G++ST+V+LGL +NLFFT PLMM PVYE+VERR    RYS+W+RW  V +V+LVA
Sbjct: 301 TNLGTGVVSTLVQLGLAINLFFTFPLMMQPVYEVVERRLCSSRYSVWVRWATVLVVTLVA 360

Query: 361 LLVPNFADFLSLVGSAVCCALAFVLPALFHFLVFKQEMDVKGWCVDIGILVLGVVLGVSG 420
           LLVPNFADFLSLVGS+VC  L FVLP+LFH   FK E+ +    VD+ + ++GV++ ++G
Sbjct: 361 LLVPNFADFLSLVGSSVCVVLGFVLPSLFHLQAFKNELSITRIVVDVLVFLIGVMIAITG 420

Query: 421 TWSALVVIFSVK 427
           TW+A+  I + K
Sbjct: 421 TWTAVHEILTSK 426

BLAST of Cp4.1LG16g03540 vs. TAIR 10
Match: AT3G11900.1 (aromatic and neutral transporter 1 )

HSP 1 Score: 240.7 bits (613), Expect = 2.1e-63
Identity = 156/440 (35.45%), Postives = 246/440 (55.91%), Query Frame = 0

Query: 4   DLEASSSSSLLQSPAVKEDTPLLGSANPLSSQPKTFANVFIAIVGAGVLGLPYAFKRTGW 63
           DL A++  S L  P +K   P   +    +S  +T  N+ ++IVG GVLGLPYAF+  GW
Sbjct: 5   DLTATTGDSSL--PLIK-SPPSETTGGDRTSALQTLGNIIVSIVGTGVLGLPYAFRIAGW 64

Query: 64  VMSLLMLFSVAFFTYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVDLLI 123
           +   L +  V F TYYCM+LL+  R K+ES  G  +  ++GDLG+   G+ GR + + LI
Sbjct: 65  LAGSLGVIIVGFATYYCMLLLIQCRDKLESEEGEEESKTYGDLGFKCMGTKGRYLTEFLI 124

Query: 124 ILSQTGFCVGYLIFIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNSIPT 183
             +Q G  V YL+FIG  ++ +++S           GL+  V ++   +P ++GL+ I +
Sbjct: 125 FTAQCGGSVAYLVFIGRNLSSIFSS----------YGLS-MVSFILILVPIEVGLSWITS 184

Query: 184 LTHLAPLSIFADVVDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGM----GVAVYA 243
           L+ L+P SIFAD+ ++ AM  V+ ++V +  +   S       S    G+    GVAV+ 
Sbjct: 185 LSALSPFSIFADICNIIAMCFVVKENVEMVIEGDFSFSDRTAISSTIGGLPFAGGVAVFC 244

Query: 244 FEGIGMVLPLESETKNKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGS 303
           FEG  M L LES  + +E F  +L   +A IT +Y  FG  GY A+G+ TKD+IT NL +
Sbjct: 245 FEGFAMTLALESSMREREAFPKLLAKVLAGITFVYVLFGFCGYMAYGDQTKDIITLNLPN 304

Query: 304 GLISTIVKLGLCVNLFFTLPLMMNPVYEIVERRF----WGGRYS---------------L 363
              +  V++GLCV L FT P+M++P+ EI+E++     W  ++                 
Sbjct: 305 NWSAIAVQIGLCVGLTFTFPIMVHPLNEIIEQKLKRIDWLQKHHNGYSNETGSVSKFAIF 364

Query: 364 WLRWMLVFMVSLVALLVPNFADFLSLVGSAVCCALAFVLPALFHFLVFKQEMDVKGWCVD 421
             R +LV  ++ +A LVP F  F SLVGS +C  ++FVLPA +H  +    ++V    +D
Sbjct: 365 TTRTLLVVGLAAIASLVPGFGTFASLVGSTLCALISFVLPASYHLTLLGPSLNVWNKSID 424

BLAST of Cp4.1LG16g03540 vs. TAIR 10
Match: AT5G15240.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 115.5 bits (288), Expect = 1.0e-25
Identity = 115/416 (27.64%), Postives = 204/416 (49.04%), Query Frame = 0

Query: 19  VKEDTPLLGSANPL--SSQPKTFANVFIAIVGAGVLGLPYAFKRTGWVMSLLMLFSVAFF 78
           + ++  LL   NP   +S  KT  +   A+ G G+L +PYA    GW +SL++LF+VA  
Sbjct: 17  IDDEESLLDDYNPQGNTSFSKTCFHGINALSGVGILSVPYALASGGW-LSLIILFTVAIT 76

Query: 79  TYYCMMLLVYTRRKIESVIGFSKINSFGDLGYTICGSPGRIIVDLLIILSQTGFCVGYLI 138
           T+YC +L+   +R +E       + S+ D+GY   G+ GR+IV + + L        +LI
Sbjct: 77  TFYCAILI---KRCMEM---DPLLRSYPDIGYKAFGNTGRVIVSIFMNLELYLVATSFLI 136

Query: 139 FIGNTMADVYNSPTATDLSPKILGLAPKVVYVWGCLPFQLGLNSIPTLTHLAPLSIFADV 198
             G+ +  ++++     +  +  G    ++ V   +   + L+++  L++++   +FA  
Sbjct: 137 LEGDNLNKLFSNVGLNFMGLEFQGKQMFIIMVALIILPSVWLDNMRILSYVSASGVFASG 196

Query: 199 VDLGAMVVVMIKDVLIYFKQSPSVEAFGGFSVFFYGMGVAVYAF-EGIGMVLP-LESETK 258
           V L ++  V   + + +      V    G +       V++YAF      V P L +  K
Sbjct: 197 VILASIFSVGAFEGVGFKNNDSEVFRLNGVAT-----SVSLYAFCYCAHPVFPTLYTSMK 256

Query: 259 NKEKFGSVLGLSMAFITVLYGAFGTLGYFAFGEDTKDMITGNLGSGLISTIVKL-GLCVN 318
           NK +F +V+ +     T +Y +   LGY  +G D +  IT NL +  +S+ V +    VN
Sbjct: 257 NKRQFSNVMIICFTICTFIYASVAVLGYLMYGSDVESQITLNLPTDKLSSKVAIWTTLVN 316

Query: 319 LFFTLPLMMNPVYEIVERRFW----GGRYS-LWLRWMLVFMVSLVALLVPNFADFLSLVG 378
                 LM+ P+ + +  RF       R S   L  +LV    +VALL+P F D +SLVG
Sbjct: 317 PIAKFALMVTPIIDAMRSRFSRVLPNKRASGFLLSTILVTSNVIVALLLPFFGDLMSLVG 376

Query: 379 SAVCCALAFVLPALFHFLVF-KQEMDVKGWCVDIGILVLGVVLGVSGTWSALVVIF 424
           + +  + + +LP L +  +  K +       V IGI + G+V+ ++GT+ A+  IF
Sbjct: 377 AFLSASASVILPCLCYLKISGKYQRLGFETLVLIGITLTGIVVVITGTYQAVKDIF 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SVG06.2e-16168.74Amino acid transporter AVT3C OS=Arabidopsis thaliana OX=3702 GN=AVT3C PE=1 SV=1[more]
F4ILY93.1e-16070.44Amino acid transporter AVT3B OS=Arabidopsis thaliana OX=3702 GN=AVT3B PE=2 SV=1[more]
Q9FKY31.8e-14460.88Amino acid transporter AVT3A OS=Arabidopsis thaliana OX=3702 GN=AVT3A PE=1 SV=1[more]
Q9SF093.0e-6235.45Amino acid transporter ANT1 OS=Arabidopsis thaliana OX=3702 GN=ANT1 PE=1 SV=1[more]
Q495N22.1e-3629.44Proton-coupled amino acid transporter 3 OS=Homo sapiens OX=9606 GN=SLC36A3 PE=2 ... [more]
Match NameE-valueIdentityDescription
XP_023512173.15.07e-302100.00amino acid transporter AVT3B-like [Cucurbita pepo subsp. pepo][more]
KAG6570973.14.16e-30199.30Amino acid transporter AVT3B, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022986978.11.19e-30099.06amino acid transporter AVT3B-like [Cucurbita maxima][more]
KAG7010811.13.42e-30099.06Amino acid transporter AVT3B, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_022943445.16.90e-30099.06amino acid transporter AVT3B-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1JI455.77e-30199.06amino acid transporter AVT3B-like OS=Cucurbita maxima OX=3661 GN=LOC111484568 PE... [more]
A0A6J1FXT73.34e-30099.06amino acid transporter AVT3B-like OS=Cucurbita moschata OX=3662 GN=LOC111448211 ... [more]
A0A0A0KFY31.14e-27590.38Aa_trans domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G118340 PE... [more]
A0A5A7U0U93.80e-27490.14Amino acid transporter ANTL1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A5D3CW426.29e-27389.91Amino acid transporter ANTL1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
Match NameE-valueIdentityDescription
AT4G38250.14.4e-16268.74Transmembrane amino acid transporter family protein [more]
AT2G42005.12.2e-16170.44Transmembrane amino acid transporter family protein [more]
AT5G65990.11.3e-14560.88Transmembrane amino acid transporter family protein [more]
AT3G11900.12.1e-6335.45aromatic and neutral transporter 1 [more]
AT5G15240.11.0e-2527.64Transmembrane amino acid transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013057Amino acid transporter, transmembrane domainPFAMPF01490Aa_transcoord: 34..418
e-value: 1.7E-72
score: 244.4
NoneNo IPR availablePANTHERPTHR22950:SF529AMINO ACID TRANSPORTER AVT3Bcoord: 7..424
NoneNo IPR availablePANTHERPTHR22950AMINO ACID TRANSPORTERcoord: 7..424

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG16g03540.1Cp4.1LG16g03540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0003333 amino acid transmembrane transport
biological_process GO:0009734 auxin-activated signaling pathway
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005774 vacuolar membrane
molecular_function GO:0015171 amino acid transmembrane transporter activity
molecular_function GO:0015293 symporter activity