Cp4.1LG16g03410 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG16g03410 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 968.4 bits (2502), Expect = 5.9e-281
Identity = 542/923 (58.72%), Postives = 677/923 (73.35%), Query Frame = 0

Query: 50  MAVSDASDRKEVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSKRNNGE- 109
           MAV DA+DR+EVIVK+D  + ++N       S  + G+IWR+ SYDFW D +   N G  
Sbjct: 1   MAV-DAADRREVIVKIDGENGNNNG-----VSGETVGKIWRDGSYDFWTDGEGNLNKGHN 60

Query: 110 ----------GSNGDGGKGEDFEFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEM 169
                      + G+  K E FEFR+     EDPP+KLIGQFLHKQ+ASGE+CLDMD+ M
Sbjct: 61  AAAVDSDRSAATTGEQQKDEGFEFRRG----EDPPTKLIGQFLHKQQASGEICLDMDLGM 120

Query: 170 LELPHDKTLPTVAESPMRRSSKELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQ 229
            EL   + L  V+ESP R S+K                         PV          +
Sbjct: 121 DEL-QSRGLTPVSESP-RVSTKR-----------------------DPVG---------R 180

Query: 230 RDHRPHGNGHDDGAAEVVRCTSNSSFERNVSFQRKSS-LLKTKTKSRLLDPPEHQ----- 289
           RD R + N +DDG  EVV+C+ N     N   QR SS LLK +T+SRL DPP  Q     
Sbjct: 181 RDSRSNTNNNDDG--EVVKCSGN-----NAPIQRSSSTLLKMRTRSRLSDPPTPQLPPQT 240

Query: 290 -DRRSGRVPKSGQIRSGLLSKAL----DEEDDDPFLEEDLPDEYKKANLGVLTMLQWASL 349
            D +SGR+PKSGQ++SG   K+     +EE+DDPF  EDLP+EY+K  L +  +L+W SL
Sbjct: 241 ADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSL 300

Query: 350 ILIIAALICTLTIRYWRRKKLWKLELWKWEVMILVLICGRLFSGWGIRIIVFFIERSFLL 409
           ILIIA  +CTL I   R+KKLW+L+LWKWE M+LVLICGRL S W ++I+VFFIER+FLL
Sbjct: 301 ILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLL 360

Query: 410 RKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSDALEYVTKVLICLLVSTL 469
           RKRVLYFVYGVRKAVQNCLWLGLVL+AW+FLFD+KV +   + AL  VTK+ +CLLV  L
Sbjct: 361 RKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCLLVGFL 420

Query: 470 VWLVKTLIVKVLASSFHVSTYFDRIQDALYNQYVIETLSGPPLIEIQKSKEEEEKLAEEV 529
           +WLVKTL+VKVLASSFH+STYFDRIQ++L+ QYVIETLSGPPLIEIQK++EEEE+++ EV
Sbjct: 421 LWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVEV 480

Query: 530 MKLQNAGATIPPDLRASAFSSPEGGRVIGSGGLQKSP--RGRSAKLSRALSKKG-----D 589
            K QN G                   V    G QKSP   G+S  LS  LS  G     +
Sbjct: 481 KKFQNPGG------------------VEIQSGAQKSPMKTGKSPFLSHVLSNGGGGGGEN 540

Query: 590 EGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQIKDSAHEDESTTQIKSEYEAK 649
           +GITID LH+L+PKNVSAW MKRLMNI+R+G+L+TLDEQ++D + +D+   QI+SE+EAK
Sbjct: 541 KGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFEAK 600

Query: 650 VAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFEGASESRKISKSSLKNWVVNA 709
           +AA++IF NVA  GSK+IY  D+MRF+ +DEA KT+SLFEGASE+ +ISKSSLKNWVVNA
Sbjct: 601 LAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNA 660

Query: 710 FRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLVA 769
           FRERRALALTLNDTKTAVN+LH+MVNI+V +IILVIWL+ILGI ++KFL+ ++SQ+V+VA
Sbjct: 661 FRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVA 720

Query: 770 FVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIIFPN 829
           F+FGN CK VFE+II+LFV+HPFDVGDRCEIDGVQM+VEEMNILTTVFLRFDNQK+++PN
Sbjct: 721 FIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPN 780

Query: 830 SVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQRILGFIEGKKEHWCPAPLIIL 889
           S+L TK+I NYYRSPDMGD +EF +HI+TP EKI +++QRI  +IEGKK+HW PAP+I+ 
Sbjct: 781 SLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVF 840

Query: 890 KDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVKIFQELDLQYRLLPLNINVCS 939
           KD+E LN +RIA+W THRMNHQDMGE+W RR+ LVEE+ KI +ELD++YRL PL+INV +
Sbjct: 841 KDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYPLDINVRN 853

BLAST of Cp4.1LG16g03410 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 921.8 bits (2381), Expect = 6.3e-267
Identity = 507/918 (55.23%), Postives = 664/918 (72.33%), Query Frame = 0

Query: 50  MAVSDASDRKEVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSKRN-NGE 109
           MA  D++DR++ IV ++    S    A    S    G IW+ESSYDFW+    K +  G+
Sbjct: 1   MAAVDSTDRRDFIVNIN-GQESGAVGATGSSSNAEGGNIWKESSYDFWDGEKGKNDKKGD 60

Query: 110 GSNGDGGKGEDFEFRQQRK------DVEDPPSKLIGQFLHKQKASG-EMCLDMDMEMLEL 169
             + DGG    F FRQ+ +      ++ DPPSKLIGQFLHKQ+ASG E+ LD+++ M EL
Sbjct: 61  DEDEDGG---SFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAEL 120

Query: 170 -PHDKTLPTVAESPMRRSSKELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRD 229
             +    P  A +  RR    +  S   +    ++D++RRR        ++R       D
Sbjct: 121 QSNTPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRR--------QNRTSLGGSSD 180

Query: 230 HRPHGNGHDDGAAEVVRCTSNSSFERNVSFQRKSSLLKTKTKSRLLDPP-------EHQD 289
                 G +   AEV++C S           +K  L + KTKSRL DPP       +  +
Sbjct: 181 E----EGRNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQDPPTPTHPAIDKTE 240

Query: 290 RRSGRVPKSGQIRSGLLSKA-----------LDEEDDDPFLEEDLPDEYKKANLGVLTML 349
            +SGR  +SG  +SG L K+            +EE++DPFL+EDLP+E+K+  L     L
Sbjct: 241 MKSGR--RSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKLSFWVFL 300

Query: 350 QWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMILVLICGRLFSGWGIRIIVFFIE 409
           +W SL+LI+ +L+C+LTI   +RK  WKL+LWKWEV +LVLICGRL S W +RIIVF +E
Sbjct: 301 EWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVE 360

Query: 410 RSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSDALEYVTKVLICL 469
           ++F  RKRVLYFVYGVRK+VQNCLWLGLVL+AW+FLFD KV+RE +S AL YVT+VL+CL
Sbjct: 361 KNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYVTRVLVCL 420

Query: 470 LVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQYVIETLSGPPLIEIQKSKEEEEK 529
           LV+ ++WLVKT++VKVLASSFH+STYFDRIQ++L+ QYVIETLSGPPL+EIQ+ +EEE++
Sbjct: 421 LVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEEQQ 480

Query: 530 LAEEVMKLQN-AGATIPPDLRASAFSSPEGGRVIGSGGLQKSPRGRSAKLSRALSKKGD- 589
           +AE+V  L+  AGA +PP L+A+  S  +               G+S  L+R  SK+G+ 
Sbjct: 481 VAEDVKSLEKLAGAKLPPALKATVKSFMK--------------VGKSPGLNRIGSKRGED 540

Query: 590 -EGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQIKDSAHEDESTTQIKSEYEA 649
            EGI ID L R++ KNVSAWNMKRLMNI+  GA+STLD+ ++D+  EDE  T I+SEYEA
Sbjct: 541 GEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSEYEA 600

Query: 650 KVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFEGASESRKISKSSLKNWVVN 709
           K AA++IF NV   GS+YIYLED +RF+ E+EA + M+LFEGASES KISKS LKNWVV 
Sbjct: 601 KCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVK 660

Query: 710 AFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLV 769
           AFRERRALALTLNDTKTAV++LHR++N+++ +II++IWLLILGIAT++FLL ++SQL+LV
Sbjct: 661 AFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLV 720

Query: 770 AFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIIFP 829
           AFVFGN+CKT+FEAIIFLFVMHPFDVGDRCEIDGVQ++VEEMNILTTVFLR+DNQKII+P
Sbjct: 721 AFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYP 780

Query: 830 NSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQRILGFIEGKKEHWCPAPLII 889
           NSVL TK I NYYRSPDMGDAVEFC+HI+TPPEKI  ++QRIL +++ KK++W PAP+I+
Sbjct: 781 NSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIV 840

Query: 890 LKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVKIFQELDLQYRLLPLNINVC 934
              +++LN ++IA+WLTHRMNHQDMGER+ RR  L+EE+ K  +ELD++YRL PLNINV 
Sbjct: 841 FLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNINVR 875

BLAST of Cp4.1LG16g03410 vs. ExPASy Swiss-Prot
Match: F4IME2 (Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana OX=3702 GN=MSL8 PE=2 SV=2)

HSP 1 Score: 917.5 bits (2370), Expect = 1.2e-265
Identity = 517/962 (53.74%), Postives = 690/962 (71.73%), Query Frame = 0

Query: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKRSFMAVSDASDRKE 60
           MDF   +FKSH SYK +R   G  S    E LPIL D  P++     S M V D      
Sbjct: 1   MDFR-NSFKSHSSYKQIRS-PGDQSEPSPEHLPILHDHHPDH-----SGMVVDDQ----- 60

Query: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSKRNNGEGSNGDGGKGE-- 120
              K D   +S +    A    ++S + W++++     D  + R + +       KG+  
Sbjct: 61  ---KPDSTRSSLDDGRNAPVERDASYKFWQDNTTGTSTDHTAVRTSDKDPIAISRKGDRL 120

Query: 121 --DFEFRQQRKDVEDPPSKLI-GQFLHKQ---KASGEMCLDMDMEMLELPHDKTLPTVAE 180
              F+F   +  V++ P+K++ G+ +++Q   + + E+ LD+D E  ++ H +T+PT   
Sbjct: 121 SGSFDFVHGKLPVDESPTKMVAGEPVNRQWRGRNNEEITLDVDQENDDVSH-QTMPTPTS 180

Query: 181 SPMRR--SSKELKVSF----------------ESISEISESDSLRRRHRDSPVDEEHRVQ 240
           +      +S+E++VSF                 S    S S +  R ++D P  +E    
Sbjct: 181 TARTSFDASREMRVSFNVRRAGGAFVAGSVPSSSSHSSSSSSATMRTNQDQPQLQEE--- 240

Query: 241 QPPQRDHRPHGNGHDDGAAEVVRCTSNSSFERNVSFQRKSSLL-KTKTKSRLLDPP-EHQ 300
                              EVVRCTS      N+SFQRKS L+ + KT+SRL DPP E +
Sbjct: 241 -------------------EVVRCTS------NMSFQRKSELISRVKTRSRLQDPPREEE 300

Query: 301 DRRSGRVPKSGQIRSGLLSKALDEEDDDPFLEEDLPDEYKKANLGVLTMLQWASLILIIA 360
              SG   +SGQ++SGLL+    +E+DDP  EED+PDEYK+  L  +T+LQW SL+ IIA
Sbjct: 301 TPYSGW--RSGQLKSGLLADI--DEEDDPLAEEDVPDEYKRGKLDAITLLQWLSLVAIIA 360

Query: 361 ALICTLTIRYWRRKKLWKLELWKWEVMILVLICGRLFSGWGIRIIVFFIERSFLLRKRVL 420
           AL C+L+I+ W++ ++W L LWKWEV +LVLICGRL SGWGIRI+VFFIER+FLLRKRVL
Sbjct: 361 ALACSLSIQSWKKVRVWNLHLWKWEVFLLVLICGRLVSGWGIRIVVFFIERNFLLRKRVL 420

Query: 421 YFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSDALEYVTKVLICLLVSTLVWLVK 480
           YFVYGVR+AVQNCLWLGLVL+AW+FLFD KVQRE +S  L YVTK+L+C L+ST++WL+K
Sbjct: 421 YFVYGVRRAVQNCLWLGLVLLAWHFLFDKKVQRETRSRFLPYVTKILVCFLLSTILWLIK 480

Query: 481 TLIVKVLASSFHVSTYFDRIQDALYNQYVIETLSGPPLIEIQKSKEEEEKLAEEVMKLQN 540
           TL+VKVLASSFHVSTYFDRIQ+AL+NQYVIETLSGPP+IE+ + +EEEE+  +E+ K+QN
Sbjct: 481 TLVVKVLASSFHVSTYFDRIQEALFNQYVIETLSGPPMIEMSRIEEEEERAQDEIFKMQN 540

Query: 541 AGATIPPDLRASAFSSPEGGRVIGSGGLQKSPRGRSAKLSRALSKK-GDEGITIDHLHRL 600
           AGA +PPDL A+AF   + GRV+            + KLS  + K   D GI+++HLHR+
Sbjct: 541 AGANLPPDLCAAAFPPGKSGRVM------------NPKLSPIIPKSTTDNGISMEHLHRM 600

Query: 601 SPKNVSAWNMKRLMNIVRHGALSTLDEQIKDSAHEDESTTQIKSEYEAKVAAKRIFQNVA 660
           + KN+SAWNMKRLM IVR+ +L+TLDEQ+ +S +EDEST QI+SE EAK AA++IF+NV 
Sbjct: 601 NHKNISAWNMKRLMKIVRNVSLTTLDEQMLESTYEDESTRQIRSEKEAKAAARKIFKNVE 660

Query: 661 SQGSKYIYLEDLMRFMVEDEASKTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTL 720
            +G+KYIYLEDLMRF+ EDEA KTM LFEGA E+++ISKS+LKNW+VNAFRERRALALTL
Sbjct: 661 QRGAKYIYLEDLMRFLREDEAMKTMGLFEGAPENKRISKSALKNWLVNAFRERRALALTL 720

Query: 721 NDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVF 780
           NDTKTAVNKLH M+NI+ +++I+VIWL++L IA+SK LLFV+SQ+VL+AF+FGNT KTVF
Sbjct: 721 NDTKTAVNKLHHMINIVTAIVIVVIWLVLLEIASSKVLLFVSSQVVLLAFIFGNTVKTVF 780

Query: 781 EAIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIIFPNSVLATKAIHNY 840
           E+IIFLF++HP+DVGDRCEID VQ++VEEMNILTTVFLR+DN KI++PNS+L  K+I+NY
Sbjct: 781 ESIIFLFIVHPYDVGDRCEIDSVQLVVEEMNILTTVFLRYDNLKIMYPNSLLWQKSINNY 840

Query: 841 YRSPDMGDAVEFCLHISTPPEKIAIMRQRILGFIEGKKEHWCPAPLIILKDVEELNRMRI 900
           YRSPDMGDA+EFC+HI+TP EKI++++QRI  +I+ K E+W P   II+KD+E+L+ +R+
Sbjct: 841 YRSPDMGDAIEFCVHITTPLEKISVIKQRISNYIDNKPEYWYPQAKIIVKDLEDLHIVRL 900

Query: 901 AIWLTHRMNHQDMGERWTRRAFLVEELVKIFQELDLQYRLLPLNINVCSLPPVNSTRLPA 934
           AIW  HR+NHQDM ERWTRRA LVEE++KI  ELD+Q+R  PL+INV ++P V S+R+P 
Sbjct: 901 AIWPCHRINHQDMAERWTRRAVLVEEVIKILLELDIQHRFYPLDINVRTMPTVVSSRVPP 902

BLAST of Cp4.1LG16g03410 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 914.1 bits (2361), Expect = 1.3e-264
Identity = 521/926 (56.26%), Postives = 659/926 (71.17%), Query Frame = 0

Query: 50  MAVSDASDRKEVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFW-NDSDSKRNNGE 109
           MAV     R++ +V++D  D               S + WRESS +FW ND  SK   GE
Sbjct: 1   MAVDSTDQRRDFVVRIDGEDN------------GDSEKFWRESSINFWHNDKSSKPPGGE 60

Query: 110 GSNGDGGKGEDFEFRQQRKDVE-DPPSKLIGQFLHKQKASG-EMCLDMDMEMLELPHDKT 169
             +G      DF  R   K  E DPPSKLI QFL+KQKASG E+ LDM+  M EL    T
Sbjct: 61  EDDG----SFDFMRRSSEKSEEPDPPSKLINQFLNKQKASGDEISLDMEANMPEL-QKNT 120

Query: 170 LPTVAESPMRRSSKELKVSFESISEISESDSLRRRHR-----------DSPVDEEHRVQQ 229
           +P ++ + +  S+  +     +       D++RRR             DS  DEE+RV  
Sbjct: 121 VPPLSSTAVSGSASPVTAPVTASYRNGTGDAIRRRQNRVTLSPSVKDGDSSEDEENRV-- 180

Query: 230 PPQRDHRPHGNGHDDGAAEVVRCTSNSSFERNVSFQRKSSLLKTKTKSRLLDP--PEHQD 289
                         DG +EVV+CTSN       S  R  +L+K KT+SRL+DP  P + D
Sbjct: 181 --------------DG-SEVVKCTSNR------STMRTKTLMKMKTRSRLMDPPTPTYPD 240

Query: 290 RRSGRVPKSGQIRSGLL-------------SKALDEEDDDPFLEEDLPDEYKKANLGVLT 349
             SGR P+SG +  G               SK L EE++DPF EEDLP+  +K  + V  
Sbjct: 241 MVSGRTPRSGNLNPGFSGRNTKPGTPNQGGSKDL-EEEEDPFSEEDLPEGLRKEKICVWV 300

Query: 350 MLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMILVLICGRLFSGWGIRIIVFF 409
           +++W  LILIIA+LIC+L I Y R K LW L LWKWEVM+LVLICGRL S W +++ V+F
Sbjct: 301 IIEWIFLILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYF 360

Query: 410 IERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSDALEYVTKVLI 469
           +E +FL RK+VLYFVYG+RK VQNCLWLGLVLIAW+FLFD KV+RE++S  L+YVTKVLI
Sbjct: 361 VESNFLWRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLI 420

Query: 470 CLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQYVIETLSGPPLIEIQKSKEEE 529
           CLLV+ ++WL+KTL+VKVLASSFH+STYFDRIQ++L+ QYVIETLSGPP IEI     EE
Sbjct: 421 CLLVAVIIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEIH---IEE 480

Query: 530 EKLAEEVMKLQNAGATIPPDLRASAFSSPEGGRVIGSGGLQKSPR--GRSAKLSRALSKK 589
           EK+A +V   +  G  + P L   A SSP     +GSG LQKSP   G+S  LSR+ SKK
Sbjct: 481 EKVANDVKTFEIVGRKLSP-LGPKAVSSPP-QVTVGSGRLQKSPSRVGKSPVLSRSGSKK 540

Query: 590 --GDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQIKD-SAHEDESTTQIKS 649
             G+EGI IDHL R++ KNVSAW MK+LMN+++ G LSTLDEQI+D +  ED+  TQI+S
Sbjct: 541 EGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRS 600

Query: 650 EYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFEGASESRKISKSSLKN 709
           E+EAK+AA++IFQNVA  GS+YIY+ED MRF+ EDE+ + M LFEGASE  KISKS LKN
Sbjct: 601 EFEAKLAARKIFQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKN 660

Query: 710 WVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQ 769
           WVVNAFRERRALALTLNDTKTAVN+LHR+V++LVS++IL+IWLLILGIAT+KFLL ++SQ
Sbjct: 661 WVVNAFRERRALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQ 720

Query: 770 LVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQK 829
           L+LV FVFGN+CKT+FEA+IF+FVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQK
Sbjct: 721 LLLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQK 780

Query: 830 IIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQRILGFIEGKKEHWCPA 889
           I++PNS+L TK I NYYRSPDM DA+EF +HI+TPPEK   +RQRIL +++ KK+HW P+
Sbjct: 781 IVYPNSLLGTKPIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPS 840

Query: 890 PLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVKIFQELDLQYRLLPLN 939
           P+I+ +D+  LN ++IA+W TH+MNHQ+MGER+ RR  L+EE+ ++ +ELD++YRL PLN
Sbjct: 841 PMIVFRDMCGLNSVKIAMWPTHKMNHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLN 880

BLAST of Cp4.1LG16g03410 vs. ExPASy Swiss-Prot
Match: F4IME1 (Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana OX=3702 GN=MSL7 PE=2 SV=1)

HSP 1 Score: 801.6 bits (2069), Expect = 9.6e-231
Identity = 455/886 (51.35%), Postives = 611/886 (68.96%), Query Frame = 0

Query: 53  SDASDRKEVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSKRNNGEGSNG 112
           ++ + +K+ +  +D+ D         +    SSG  +  +SY FW D          +  
Sbjct: 23  NEKTKKKKKLANLDDGD---------IAKTQSSGSSFDGNSYKFWQDI---------ATD 82

Query: 113 DGGKGEDFEFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPHDKTLPTVAE 172
           D  K   F+F Q R+                     E+ LD++ E  E         V+ 
Sbjct: 83  DYTKSGSFDFPQYRE---------------------EITLDVNEETEE------TEDVSN 142

Query: 173 SPMRRSSKELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRDHRPHGNGHDDGA 232
           +     SKE +V F+  S  + + S   R   S         +             +D  
Sbjct: 143 NNNLSGSKETRVFFKINSSGTNNMSGSVRSCTSSTSFSSATMRLNLEQQL------EDEG 202

Query: 233 AEVVRCTSNSSFERNVSFQRKSSLL-KTKTKSRLLDPPEHQDRR-SGRVPKSGQIRSGLL 292
             VVRC+S           RK+ L+ + K +SRL+DPP+ ++++ S  +  S Q+RSGLL
Sbjct: 203 EVVVRCSS----------VRKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLL 262

Query: 293 SKALD--EEDDDPFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKL 352
            +  D  EE+DD   EED+P EY+K  +  +T+LQW SLI ++ AL+ +L +  WR   L
Sbjct: 263 GRHSDDIEEEDDSSAEEDVPVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATL 322

Query: 353 WKLELWKWEVMILVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWL 412
           W L LWKWEV++LVLICGRL SG GIRIIVFFIER+FLLRKRVLYFVYGV+ AVQNCLWL
Sbjct: 323 WSLHLWKWEVVLLVLICGRLVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWL 382

Query: 413 GLVLIAWNFLFDDKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTY 472
           GLVL+AW+FLFD KV++E +SD L  ++K+L+C L+ST++WL+KTL+VKVLASSFHVSTY
Sbjct: 383 GLVLLAWHFLFDKKVEKETQSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTY 442

Query: 473 FDRIQDALYNQYVIETLSGPPLIEIQKSKEEEEKLAEEVMKLQNAGATIPPDLRASAFSS 532
           FDRIQ+AL++ Y+IETLSGPP++E+ + +EEE++  +E+ K+Q  GA + P+L ++AF  
Sbjct: 443 FDRIQEALFHHYLIETLSGPPMLELSRIEEEEDRTQDEIYKMQKGGADLSPELCSAAFPQ 502

Query: 533 PEGGRVIGSGGLQKSPRGRSAKLSRALSKKG-DEGITIDHLHRLSPKNVSAWNMKRLMNI 592
            + G  +            + K S  + K G D GIT+D LH+++ KNVSAWNMKRLM I
Sbjct: 503 EKSGSTM------------NMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKI 562

Query: 593 VRHGALSTLDEQIKDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFM 652
           VR+ +LSTLDEQ   +  EDEST QI+SE EAK AA++IF+NVA  G+K+IYLEDLMRF+
Sbjct: 563 VRNVSLSTLDEQALQNTCEDESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFL 622

Query: 653 VEDEASKTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNI 712
             DEA KTM LFEGA  ++KI+KS+LKNW+VNAFRERRALALTLNDTKTAVNKLH M++ 
Sbjct: 623 RVDEAMKTMCLFEGALVTKKITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISF 682

Query: 713 LVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGD 772
           L +++I+VIWL++L IATSK+LLF+TSQ+VL+AF+FGN+ KTVFE+IIFLF++HP+DVGD
Sbjct: 683 LTAIVIIVIWLILLEIATSKYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGD 742

Query: 773 RCEIDGVQMIVEEMNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHI 832
           R  ID V+M+VEEMNILTTVFLR DN KI++PN +L  KAIHNY RSPDMGD V  C+HI
Sbjct: 743 RLLIDTVEMVVEEMNILTTVFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHI 802

Query: 833 STPPEKIAIMRQRILGFIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGER 892
           +TPPEKIA ++QRI  +I+ K E+W P   +I+KDVE+LN +RIAIWL H++NHQ+MGER
Sbjct: 803 TTPPEKIAAIKQRISSYIDSKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGER 835

Query: 893 WTRRAFLVEELVKIFQELDLQYRLLPLNINVCSLPPVNSTRLPATW 934
           +TRRA L+EE++KI  ELD+QYR  PL+INV ++P V S+R+P  W
Sbjct: 863 FTRRALLIEEVIKILLELDIQYRFHPLDINVKTMPTVVSSRVPPAW 835

BLAST of Cp4.1LG16g03410 vs. NCBI nr
Match: XP_023512518.1 (mechanosensitive ion channel protein 6-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023512519.1 mechanosensitive ion channel protein 6-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1804 bits (4673), Expect = 0.0
Identity = 938/938 (100.00%), Postives = 938/938 (100.00%), Query Frame = 0

Query: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKRSFMAVSDASDRKE 60
           MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKRSFMAVSDASDRKE
Sbjct: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKRSFMAVSDASDRKE 60

Query: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSKRNNGEGSNGDGGKGEDF 120
           VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSKRNNGEGSNGDGGKGEDF
Sbjct: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSKRNNGEGSNGDGGKGEDF 120

Query: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPHDKTLPTVAESPMRRSSK 180
           EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPHDKTLPTVAESPMRRSSK
Sbjct: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPHDKTLPTVAESPMRRSSK 180

Query: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRDHRPHGNGHDDGAAEVVRCTS 240
           ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRDHRPHGNGHDDGAAEVVRCTS
Sbjct: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRDHRPHGNGHDDGAAEVVRCTS 240

Query: 241 NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD 300
           NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD
Sbjct: 241 NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD 300

Query: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360
           PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI
Sbjct: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360

Query: 361 LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420
           LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD
Sbjct: 361 LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420

Query: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480
           DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY
Sbjct: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480

Query: 481 VIETLSGPPLIEIQKSKEEEEKLAEEVMKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540
           VIETLSGPPLIEIQKSKEEEEKLAEEVMKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL
Sbjct: 481 VIETLSGPPLIEIQKSKEEEEKLAEEVMKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540

Query: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600
           QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI
Sbjct: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600

Query: 601 KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660
           KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE
Sbjct: 601 KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660

Query: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720
           GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI
Sbjct: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720

Query: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780
           LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE
Sbjct: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780

Query: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840
           MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR
Sbjct: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840

Query: 841 ILGFIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900
           ILGFIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK
Sbjct: 841 ILGFIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900

Query: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938
           IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS
Sbjct: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938

BLAST of Cp4.1LG16g03410 vs. NCBI nr
Match: KAG6571002.1 (Mechanosensitive ion channel protein 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1792 bits (4641), Expect = 0.0
Identity = 931/938 (99.25%), Postives = 935/938 (99.68%), Query Frame = 0

Query: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKRSFMAVSDASDRKE 60
           MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQK+SFMAVSDASDRKE
Sbjct: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60

Query: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSKRNNGEGSNGDGGKGEDF 120
           VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDS+RNNGEGSNGDGGKGEDF
Sbjct: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGEDF 120

Query: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPHDKTLPTVAESPMRRSSK 180
           EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELP DKTLPTVAESPMRRSSK
Sbjct: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPTVAESPMRRSSK 180

Query: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRDHRPHGNGHDDGAAEVVRCTS 240
           ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQ PPQRDHRPHGNGHDDGAAEVVRCTS
Sbjct: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPQRDHRPHGNGHDDGAAEVVRCTS 240

Query: 241 NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD 300
           NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD
Sbjct: 241 NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD 300

Query: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360
           PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI
Sbjct: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360

Query: 361 LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420
           LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD
Sbjct: 361 LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420

Query: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480
           DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY
Sbjct: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480

Query: 481 VIETLSGPPLIEIQKSKEEEEKLAEEVMKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540
           VIETLSGPPLIEIQKSKEEEEKLAEEVMKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL
Sbjct: 481 VIETLSGPPLIEIQKSKEEEEKLAEEVMKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540

Query: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600
           QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI
Sbjct: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600

Query: 601 KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660
           KDSAHEDESTTQIKSEYEAKVAAK+IFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE
Sbjct: 601 KDSAHEDESTTQIKSEYEAKVAAKKIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660

Query: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720
           GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI
Sbjct: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720

Query: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780
           LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE
Sbjct: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780

Query: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840
           MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR
Sbjct: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840

Query: 841 ILGFIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900
           IL +IEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK
Sbjct: 841 ILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900

Query: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938
           IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS
Sbjct: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938

BLAST of Cp4.1LG16g03410 vs. NCBI nr
Match: XP_022943801.1 (mechanosensitive ion channel protein 6-like [Cucurbita moschata])

HSP 1 Score: 1783 bits (4617), Expect = 0.0
Identity = 927/938 (98.83%), Postives = 932/938 (99.36%), Query Frame = 0

Query: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKRSFMAVSDASDRKE 60
           MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQK+SFMAVSDASDRKE
Sbjct: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60

Query: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSKRNNGEGSNGDGGKGEDF 120
           VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDS+RNNGEGSNGDGGKGEDF
Sbjct: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGEDF 120

Query: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPHDKTLPTVAESPMRRSSK 180
           EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELP DKTLPTVAESPMRRSSK
Sbjct: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPTVAESPMRRSSK 180

Query: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRDHRPHGNGHDDGAAEVVRCTS 240
           ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQ PP RDHRPHGNGHDDGAAEVV CTS
Sbjct: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPLRDHRPHGNGHDDGAAEVVSCTS 240

Query: 241 NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD 300
           NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD
Sbjct: 241 NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD 300

Query: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360
           PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI
Sbjct: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360

Query: 361 LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420
           LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD
Sbjct: 361 LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420

Query: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480
           DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY
Sbjct: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480

Query: 481 VIETLSGPPLIEIQKSKEEEEKLAEEVMKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540
           VIETLSGPPLIEIQK+KEEE KLAEEV+KLQNAGATIPPDLRASAFSSPEGGRVIGSGGL
Sbjct: 481 VIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540

Query: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600
           QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI
Sbjct: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600

Query: 601 KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660
           KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE
Sbjct: 601 KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660

Query: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720
           GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI
Sbjct: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720

Query: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780
           LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE
Sbjct: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780

Query: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840
           MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR
Sbjct: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840

Query: 841 ILGFIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900
           IL +IEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK
Sbjct: 841 ILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900

Query: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938
           IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS
Sbjct: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938

BLAST of Cp4.1LG16g03410 vs. NCBI nr
Match: XP_022985632.1 (mechanosensitive ion channel protein 6-like [Cucurbita maxima])

HSP 1 Score: 1756 bits (4547), Expect = 0.0
Identity = 912/938 (97.23%), Postives = 923/938 (98.40%), Query Frame = 0

Query: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKRSFMAVSDASDRKE 60
           MDFSLKAFKSHGSYKYVRKLSGTD+TQDQEQLPILSDQEPNNLHQK+SFMAVSDASDRKE
Sbjct: 1   MDFSLKAFKSHGSYKYVRKLSGTDTTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60

Query: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSKRNNGEGSNGDGGKGEDF 120
           VIVKVD+AD SSNASAR VESVNSSGRIWRESSYDFWNDS  +RNNGEGS+GDGGKGEDF
Sbjct: 61  VIVKVDQADVSSNASARPVESVNSSGRIWRESSYDFWNDSGRRRNNGEGSDGDGGKGEDF 120

Query: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPHDKTLPTVAESPMRRSSK 180
           EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELP DKTLP V ESP+RRSSK
Sbjct: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPPVVESPLRRSSK 180

Query: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRDHRPHGNGHDDGAAEVVRCTS 240
           ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRDHR HGNGHDDG  EVVRCTS
Sbjct: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRDHRAHGNGHDDGTPEVVRCTS 240

Query: 241 NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD 300
           NSSFER VSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSG+IRSGLLSKALDEEDDD
Sbjct: 241 NSSFERGVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGKIRSGLLSKALDEEDDD 300

Query: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360
           PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI
Sbjct: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360

Query: 361 LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420
           LVLICGRLFSGWGIRIIVF IERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD
Sbjct: 361 LVLICGRLFSGWGIRIIVFLIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420

Query: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480
           DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY
Sbjct: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480

Query: 481 VIETLSGPPLIEIQKSKEEEEKLAEEVMKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540
           VIETLSGPPLIEIQK+KEEE KLAEEV+KLQNAGATIPPDLRASAFSSPEGGRVIGSGGL
Sbjct: 481 VIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540

Query: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600
           QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI
Sbjct: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600

Query: 601 KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660
           KDSAHEDESTTQIKSEYEAKVAAK+IFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE
Sbjct: 601 KDSAHEDESTTQIKSEYEAKVAAKKIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660

Query: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720
           GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI
Sbjct: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720

Query: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780
           LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE
Sbjct: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780

Query: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840
           MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR
Sbjct: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840

Query: 841 ILGFIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900
           IL +IE KKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK
Sbjct: 841 ILSYIEDKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900

Query: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938
           IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS
Sbjct: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938

BLAST of Cp4.1LG16g03410 vs. NCBI nr
Match: XP_022140409.1 (mechanosensitive ion channel protein 6-like [Momordica charantia])

HSP 1 Score: 1574 bits (4076), Expect = 0.0
Identity = 823/945 (87.09%), Postives = 884/945 (93.54%), Query Frame = 0

Query: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQD-QEQLPILSDQEPNNLHQKRSFMAVSDASDRK 60
           MDFSLK+FKSHGSYKY RKLSG     D QEQLPILSDQ+P NLHQ++S MAVSDASDR 
Sbjct: 1   MDFSLKSFKSHGSYKYARKLSGGGGADDSQEQLPILSDQDPGNLHQQQS-MAVSDASDRN 60

Query: 61  EVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSKRNNGEGSNGDGGKGED 120
           EVIVK+D+ D S NA++R +E+VN+SGRIWRESSYDFWND D +R+N EGSNGDG + ED
Sbjct: 61  EVIVKIDQPD-SLNATSRVMEAVNASGRIWRESSYDFWNDGDGRRSNAEGSNGDG-RSED 120

Query: 121 FEFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPHDKTLPTVAESPMRRSS 180
           FEFRQ R+ VEDPPSKLIGQFLHKQKASGEM LDMDMEMLELP DKTLP VAESPMRRSS
Sbjct: 121 FEFRQHRQGVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTLPPVAESPMRRSS 180

Query: 181 KELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRDHRPHGNGHDDGAAEVVRCT 240
           KELKVSFESISEISE+DS+RRRHRDSP DEE++ QQPPQRD R +GNGHDDG AEVVRCT
Sbjct: 181 KELKVSFESISEISENDSMRRRHRDSPADEEYKGQQPPQRDRRAYGNGHDDGGAEVVRCT 240

Query: 241 SNSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSG----RVP-KSGQIRSGLLSKAL 300
           SNSSF+R+VSFQRKSSLL+ KTKSRLLDPPE  DRRSG    RVP KSGQIRSGLLSKAL
Sbjct: 241 SNSSFQRDVSFQRKSSLLRAKTKSRLLDPPEQPDRRSGVRSGRVPPKSGQIRSGLLSKAL 300

Query: 301 DEEDDDPFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELW 360
           DEEDDDPFLEEDLPDEYKK+NLG LT+LQWASLILIIAAL+CTL+I Y  RK LWKL+LW
Sbjct: 301 DEEDDDPFLEEDLPDEYKKSNLGALTLLQWASLILIIAALVCTLSIPYLNRKNLWKLKLW 360

Query: 361 KWEVMILVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIA 420
           KWEVM+LVLICGRL SGWGIRIIVFFIER+FLLRKRVLYFVYGVRKAVQNCLWLGLVLIA
Sbjct: 361 KWEVMVLVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIA 420

Query: 421 WNFLFDDKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQD 480
           WNFLFDDKVQREVKS+ALEYVTKVL+CLLVSTLVWLVKTL+VKVLASSFHVSTYFDRIQD
Sbjct: 421 WNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQD 480

Query: 481 ALYNQYVIETLSGPPLIEIQKSKEEEEKLAEEVMKLQNAGATIPPDLRASAFSSPEGGRV 540
           AL+NQYVIETLSGPPLIEI+K++EEEE+LAEEVMKLQNAGATIPPDL+A+AFS+ +GGRV
Sbjct: 481 ALFNQYVIETLSGPPLIEIRKNEEEEERLAEEVMKLQNAGATIPPDLKATAFSTAKGGRV 540

Query: 541 IGSGGLQKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALS 600
           IGSGGLQ+SPRGRS KLSR+LSK GD+GITIDHLH+LSPKNVSAWNMKRLMNIVRHGALS
Sbjct: 541 IGSGGLQRSPRGRSGKLSRSLSKNGDDGITIDHLHKLSPKNVSAWNMKRLMNIVRHGALS 600

Query: 601 TLDEQIKDSAHE-DESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEAS 660
           TLDEQIKDS HE DES TQIKSE EAKVAAK+IFQNVA  GSKYIYLEDLMRFM EDEA 
Sbjct: 601 TLDEQIKDSTHEEDESATQIKSECEAKVAAKKIFQNVARHGSKYIYLEDLMRFMQEDEAL 660

Query: 661 KTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVII 720
           KTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSV+I
Sbjct: 661 KTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVVI 720

Query: 721 LVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDG 780
           LVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDG
Sbjct: 721 LVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDG 780

Query: 781 VQMIVEEMNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEK 840
           VQMIVEEMNILTT+FLR+DNQKI+FPNSVLATKAIHN+YRSPDMGDAVEFCLHISTPPEK
Sbjct: 781 VQMIVEEMNILTTIFLRYDNQKIVFPNSVLATKAIHNFYRSPDMGDAVEFCLHISTPPEK 840

Query: 841 IAIMRQRILGFIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAF 900
           IA+MRQRI+ +IE KKEHWCPAP+I+ KDVEELNR+RIAIWLTHRMNHQDMGERWTRRA 
Sbjct: 841 IAVMRQRIVSYIEAKKEHWCPAPMIVFKDVEELNRLRIAIWLTHRMNHQDMGERWTRRAL 900

Query: 901 LVEELVKIFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938
           LVEEL+KIFQELDLQYRLLPL+INV +LPPVNSTRLPATW AT S
Sbjct: 901 LVEELLKIFQELDLQYRLLPLDINVRTLPPVNSTRLPATWTATTS 942

BLAST of Cp4.1LG16g03410 vs. ExPASy TrEMBL
Match: A0A6J1FSP8 (Mechanosensitive ion channel protein OS=Cucurbita moschata OX=3662 GN=LOC111448441 PE=3 SV=1)

HSP 1 Score: 1783 bits (4617), Expect = 0.0
Identity = 927/938 (98.83%), Postives = 932/938 (99.36%), Query Frame = 0

Query: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKRSFMAVSDASDRKE 60
           MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQK+SFMAVSDASDRKE
Sbjct: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60

Query: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSKRNNGEGSNGDGGKGEDF 120
           VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDS+RNNGEGSNGDGGKGEDF
Sbjct: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGEDF 120

Query: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPHDKTLPTVAESPMRRSSK 180
           EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELP DKTLPTVAESPMRRSSK
Sbjct: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPTVAESPMRRSSK 180

Query: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRDHRPHGNGHDDGAAEVVRCTS 240
           ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQ PP RDHRPHGNGHDDGAAEVV CTS
Sbjct: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPLRDHRPHGNGHDDGAAEVVSCTS 240

Query: 241 NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD 300
           NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD
Sbjct: 241 NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD 300

Query: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360
           PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI
Sbjct: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360

Query: 361 LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420
           LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD
Sbjct: 361 LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420

Query: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480
           DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY
Sbjct: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480

Query: 481 VIETLSGPPLIEIQKSKEEEEKLAEEVMKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540
           VIETLSGPPLIEIQK+KEEE KLAEEV+KLQNAGATIPPDLRASAFSSPEGGRVIGSGGL
Sbjct: 481 VIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540

Query: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600
           QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI
Sbjct: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600

Query: 601 KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660
           KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE
Sbjct: 601 KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660

Query: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720
           GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI
Sbjct: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720

Query: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780
           LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE
Sbjct: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780

Query: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840
           MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR
Sbjct: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840

Query: 841 ILGFIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900
           IL +IEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK
Sbjct: 841 ILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900

Query: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938
           IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS
Sbjct: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938

BLAST of Cp4.1LG16g03410 vs. ExPASy TrEMBL
Match: A0A6J1J5E6 (Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111483636 PE=3 SV=1)

HSP 1 Score: 1756 bits (4547), Expect = 0.0
Identity = 912/938 (97.23%), Postives = 923/938 (98.40%), Query Frame = 0

Query: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKRSFMAVSDASDRKE 60
           MDFSLKAFKSHGSYKYVRKLSGTD+TQDQEQLPILSDQEPNNLHQK+SFMAVSDASDRKE
Sbjct: 1   MDFSLKAFKSHGSYKYVRKLSGTDTTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60

Query: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSKRNNGEGSNGDGGKGEDF 120
           VIVKVD+AD SSNASAR VESVNSSGRIWRESSYDFWNDS  +RNNGEGS+GDGGKGEDF
Sbjct: 61  VIVKVDQADVSSNASARPVESVNSSGRIWRESSYDFWNDSGRRRNNGEGSDGDGGKGEDF 120

Query: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPHDKTLPTVAESPMRRSSK 180
           EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELP DKTLP V ESP+RRSSK
Sbjct: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPPVVESPLRRSSK 180

Query: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRDHRPHGNGHDDGAAEVVRCTS 240
           ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRDHR HGNGHDDG  EVVRCTS
Sbjct: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRDHRAHGNGHDDGTPEVVRCTS 240

Query: 241 NSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDDD 300
           NSSFER VSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSG+IRSGLLSKALDEEDDD
Sbjct: 241 NSSFERGVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGKIRSGLLSKALDEEDDD 300

Query: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360
           PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI
Sbjct: 301 PFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMI 360

Query: 361 LVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420
           LVLICGRLFSGWGIRIIVF IERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD
Sbjct: 361 LVLICGRLFSGWGIRIIVFLIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFD 420

Query: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480
           DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY
Sbjct: 421 DKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQY 480

Query: 481 VIETLSGPPLIEIQKSKEEEEKLAEEVMKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540
           VIETLSGPPLIEIQK+KEEE KLAEEV+KLQNAGATIPPDLRASAFSSPEGGRVIGSGGL
Sbjct: 481 VIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGGL 540

Query: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600
           QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI
Sbjct: 541 QKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQI 600

Query: 601 KDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660
           KDSAHEDESTTQIKSEYEAKVAAK+IFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE
Sbjct: 601 KDSAHEDESTTQIKSEYEAKVAAKKIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFE 660

Query: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720
           GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI
Sbjct: 661 GASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLI 720

Query: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780
           LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE
Sbjct: 721 LGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEE 780

Query: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840
           MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR
Sbjct: 781 MNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQR 840

Query: 841 ILGFIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900
           IL +IE KKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK
Sbjct: 841 ILSYIEDKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVK 900

Query: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938
           IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS
Sbjct: 901 IFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938

BLAST of Cp4.1LG16g03410 vs. ExPASy TrEMBL
Match: A0A6J1CFM7 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111011094 PE=3 SV=1)

HSP 1 Score: 1574 bits (4076), Expect = 0.0
Identity = 823/945 (87.09%), Postives = 884/945 (93.54%), Query Frame = 0

Query: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQD-QEQLPILSDQEPNNLHQKRSFMAVSDASDRK 60
           MDFSLK+FKSHGSYKY RKLSG     D QEQLPILSDQ+P NLHQ++S MAVSDASDR 
Sbjct: 1   MDFSLKSFKSHGSYKYARKLSGGGGADDSQEQLPILSDQDPGNLHQQQS-MAVSDASDRN 60

Query: 61  EVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSKRNNGEGSNGDGGKGED 120
           EVIVK+D+ D S NA++R +E+VN+SGRIWRESSYDFWND D +R+N EGSNGDG + ED
Sbjct: 61  EVIVKIDQPD-SLNATSRVMEAVNASGRIWRESSYDFWNDGDGRRSNAEGSNGDG-RSED 120

Query: 121 FEFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPHDKTLPTVAESPMRRSS 180
           FEFRQ R+ VEDPPSKLIGQFLHKQKASGEM LDMDMEMLELP DKTLP VAESPMRRSS
Sbjct: 121 FEFRQHRQGVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTLPPVAESPMRRSS 180

Query: 181 KELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRDHRPHGNGHDDGAAEVVRCT 240
           KELKVSFESISEISE+DS+RRRHRDSP DEE++ QQPPQRD R +GNGHDDG AEVVRCT
Sbjct: 181 KELKVSFESISEISENDSMRRRHRDSPADEEYKGQQPPQRDRRAYGNGHDDGGAEVVRCT 240

Query: 241 SNSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSG----RVP-KSGQIRSGLLSKAL 300
           SNSSF+R+VSFQRKSSLL+ KTKSRLLDPPE  DRRSG    RVP KSGQIRSGLLSKAL
Sbjct: 241 SNSSFQRDVSFQRKSSLLRAKTKSRLLDPPEQPDRRSGVRSGRVPPKSGQIRSGLLSKAL 300

Query: 301 DEEDDDPFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELW 360
           DEEDDDPFLEEDLPDEYKK+NLG LT+LQWASLILIIAAL+CTL+I Y  RK LWKL+LW
Sbjct: 301 DEEDDDPFLEEDLPDEYKKSNLGALTLLQWASLILIIAALVCTLSIPYLNRKNLWKLKLW 360

Query: 361 KWEVMILVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIA 420
           KWEVM+LVLICGRL SGWGIRIIVFFIER+FLLRKRVLYFVYGVRKAVQNCLWLGLVLIA
Sbjct: 361 KWEVMVLVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIA 420

Query: 421 WNFLFDDKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQD 480
           WNFLFDDKVQREVKS+ALEYVTKVL+CLLVSTLVWLVKTL+VKVLASSFHVSTYFDRIQD
Sbjct: 421 WNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQD 480

Query: 481 ALYNQYVIETLSGPPLIEIQKSKEEEEKLAEEVMKLQNAGATIPPDLRASAFSSPEGGRV 540
           AL+NQYVIETLSGPPLIEI+K++EEEE+LAEEVMKLQNAGATIPPDL+A+AFS+ +GGRV
Sbjct: 481 ALFNQYVIETLSGPPLIEIRKNEEEEERLAEEVMKLQNAGATIPPDLKATAFSTAKGGRV 540

Query: 541 IGSGGLQKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALS 600
           IGSGGLQ+SPRGRS KLSR+LSK GD+GITIDHLH+LSPKNVSAWNMKRLMNIVRHGALS
Sbjct: 541 IGSGGLQRSPRGRSGKLSRSLSKNGDDGITIDHLHKLSPKNVSAWNMKRLMNIVRHGALS 600

Query: 601 TLDEQIKDSAHE-DESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEAS 660
           TLDEQIKDS HE DES TQIKSE EAKVAAK+IFQNVA  GSKYIYLEDLMRFM EDEA 
Sbjct: 601 TLDEQIKDSTHEEDESATQIKSECEAKVAAKKIFQNVARHGSKYIYLEDLMRFMQEDEAL 660

Query: 661 KTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVII 720
           KTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSV+I
Sbjct: 661 KTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVVI 720

Query: 721 LVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDG 780
           LVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDG
Sbjct: 721 LVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDG 780

Query: 781 VQMIVEEMNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEK 840
           VQMIVEEMNILTT+FLR+DNQKI+FPNSVLATKAIHN+YRSPDMGDAVEFCLHISTPPEK
Sbjct: 781 VQMIVEEMNILTTIFLRYDNQKIVFPNSVLATKAIHNFYRSPDMGDAVEFCLHISTPPEK 840

Query: 841 IAIMRQRILGFIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAF 900
           IA+MRQRI+ +IE KKEHWCPAP+I+ KDVEELNR+RIAIWLTHRMNHQDMGERWTRRA 
Sbjct: 841 IAVMRQRIVSYIEAKKEHWCPAPMIVFKDVEELNRLRIAIWLTHRMNHQDMGERWTRRAL 900

Query: 901 LVEELVKIFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938
           LVEEL+KIFQELDLQYRLLPL+INV +LPPVNSTRLPATW AT S
Sbjct: 901 LVEELLKIFQELDLQYRLLPLDINVRTLPPVNSTRLPATWTATTS 942

BLAST of Cp4.1LG16g03410 vs. ExPASy TrEMBL
Match: A0A5D3DK11 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold250G00230 PE=3 SV=1)

HSP 1 Score: 1570 bits (4065), Expect = 0.0
Identity = 828/934 (88.65%), Postives = 874/934 (93.58%), Query Frame = 0

Query: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKRSFMAVSDASDRKE 60
           MDFSLK+FKSH SYKYVRKLSG D+TQDQE LPILSD      HQ  S MAVSDASDRKE
Sbjct: 1   MDFSLKSFKSHPSYKYVRKLSGADATQDQEHLPILSD------HQ-HSIMAVSDASDRKE 60

Query: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSKRNNGEGSNGDGGKGEDF 120
           VIVKVDEADASS    RA + VN SGRIWRESSYDFWNDSD++RNNGEG        EDF
Sbjct: 61  VIVKVDEADASS---LRATDLVNGSGRIWRESSYDFWNDSDNRRNNGEGG---ARTTEDF 120

Query: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPHDKT-LPTVAESPMRRSS 180
           EFRQ RKDVEDPPSKLIGQFLHKQKASGEM LDMDMEMLELP DKT L TVAESPMRRSS
Sbjct: 121 EFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSS 180

Query: 181 KELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRDHRPHG-NGHDDGAAEVVRC 240
           +ELKVSFESISEISE+DS+RRRHRDSP+DEEHR QQP Q D R HG NG DDGAAEV+RC
Sbjct: 181 RELKVSFESISEISENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRC 240

Query: 241 TSNSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEED 300
           +SNSSF+R+VSFQRKSSLL+ KTKSRLLDPPEHQDRRSGRVPKSGQ+RSGL+SK LDEED
Sbjct: 241 SSNSSFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKVLDEED 300

Query: 301 DDPFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEV 360
           DDPFLEEDLPDEYKKANLGVLT+LQWASLILIIAAL+CTLTIRYWRRKKLWKLE+WKWEV
Sbjct: 301 DDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEV 360

Query: 361 MILVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFL 420
           MILVLICGRL SGWGIR+IVFFIER+FLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFL
Sbjct: 361 MILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFL 420

Query: 421 FDDKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYN 480
           FDDKVQREVKS+ALEYVTKVL+CLL+STLVWLVKTL+VKVLASSFHVSTYFDRIQDAL+N
Sbjct: 421 FDDKVQREVKSNALEYVTKVLVCLLISTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFN 480

Query: 481 QYVIETLSGPPLIEIQKSKEEEEKLAEEVMKLQNAGATIPPDLRASAFSSPE-GGRVIGS 540
           QYVIETLSGPPLIEIQK++EEEE+LAEEVMKLQNAGATIPPDL+A+AFS+ + GGRVIGS
Sbjct: 481 QYVIETLSGPPLIEIQKNEEEEERLAEEVMKLQNAGATIPPDLKATAFSTAQKGGRVIGS 540

Query: 541 GGLQKSPRGRSAKLSRALSKKG-DEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTL 600
           GGLQKSPRGRS KLSR LSKKG DEGITIDHLH+LSPKNVSAWNMKRLMNIVRHG LSTL
Sbjct: 541 GGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTL 600

Query: 601 DEQIKDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTM 660
           DEQIKD+AHEDESTTQIKSEYEAKVAAK+IF NVA  GSKYIYLEDLMRFM +DEASKTM
Sbjct: 601 DEQIKDTAHEDESTTQIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTM 660

Query: 661 SLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVI 720
            LFEGA ESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVI
Sbjct: 661 GLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVI 720

Query: 721 WLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQM 780
           WLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFE+IIFLFVMHPFDVGDRCEIDGVQM
Sbjct: 721 WLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQM 780

Query: 781 IVEEMNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAI 840
           IVEEMNILTT+FLR+DNQKIIFPNSVLATKAIHN+YRSPDMGD VEFCLHISTPPEKIAI
Sbjct: 781 IVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGVEFCLHISTPPEKIAI 840

Query: 841 MRQRILGFIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVE 900
           MRQRI+ +IEGKKEHWCPAP+I+LKDVEELNRMRIAIWLTHRMNHQDMGERWTRRA LVE
Sbjct: 841 MRQRIVSYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVE 900

Query: 901 ELVKIFQELDLQYRLLPLNINVCSLPPVNSTRLP 930
           ELVKIFQELDLQYRLLPL+INV SLPPVNST  P
Sbjct: 901 ELVKIFQELDLQYRLLPLDINVRSLPPVNSTNFP 921

BLAST of Cp4.1LG16g03410 vs. ExPASy TrEMBL
Match: A0A1S3BIW1 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103490566 PE=3 SV=1)

HSP 1 Score: 1570 bits (4065), Expect = 0.0
Identity = 828/934 (88.65%), Postives = 874/934 (93.58%), Query Frame = 0

Query: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKRSFMAVSDASDRKE 60
           MDFSLK+FKSH SYKYVRKLSG D+TQDQE LPILSD      HQ  S MAVSDASDRKE
Sbjct: 1   MDFSLKSFKSHPSYKYVRKLSGADATQDQEHLPILSD------HQ-HSIMAVSDASDRKE 60

Query: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSKRNNGEGSNGDGGKGEDF 120
           VIVKVDEADASS    RA + VN SGRIWRESSYDFWNDSD++RNNGEG        EDF
Sbjct: 61  VIVKVDEADASS---LRATDLVNGSGRIWRESSYDFWNDSDNRRNNGEGG---ARTTEDF 120

Query: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPHDKT-LPTVAESPMRRSS 180
           EFRQ RKDVEDPPSKLIGQFLHKQKASGEM LDMDMEMLELP DKT L TVAESPMRRSS
Sbjct: 121 EFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSS 180

Query: 181 KELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRDHRPHG-NGHDDGAAEVVRC 240
           +ELKVSFESISEISE+DS+RRRHRDSP+DEEHR QQP Q D R HG NG DDGAAEV+RC
Sbjct: 181 RELKVSFESISEISENDSMRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRC 240

Query: 241 TSNSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEED 300
           +SNSSF+R+VSFQRKSSLL+ KTKSRLLDPPEHQDRRSGRVPKSGQ+RSGL+SK LDEED
Sbjct: 241 SSNSSFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKVLDEED 300

Query: 301 DDPFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEV 360
           DDPFLEEDLPDEYKKANLGVLT+LQWASLILIIAAL+CTLTIRYWRRKKLWKLE+WKWEV
Sbjct: 301 DDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEV 360

Query: 361 MILVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFL 420
           MILVLICGRL SGWGIR+IVFFIER+FLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFL
Sbjct: 361 MILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFL 420

Query: 421 FDDKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYN 480
           FDDKVQREVKS+ALEYVTKVL+CLL+STLVWLVKTL+VKVLASSFHVSTYFDRIQDAL+N
Sbjct: 421 FDDKVQREVKSNALEYVTKVLVCLLISTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFN 480

Query: 481 QYVIETLSGPPLIEIQKSKEEEEKLAEEVMKLQNAGATIPPDLRASAFSSPE-GGRVIGS 540
           QYVIETLSGPPLIEIQK++EEEE+LAEEVMKLQNAGATIPPDL+A+AFS+ + GGRVIGS
Sbjct: 481 QYVIETLSGPPLIEIQKNEEEEERLAEEVMKLQNAGATIPPDLKATAFSTAQKGGRVIGS 540

Query: 541 GGLQKSPRGRSAKLSRALSKKG-DEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTL 600
           GGLQKSPRGRS KLSR LSKKG DEGITIDHLH+LSPKNVSAWNMKRLMNIVRHG LSTL
Sbjct: 541 GGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTL 600

Query: 601 DEQIKDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTM 660
           DEQIKD+AHEDESTTQIKSEYEAKVAAK+IF NVA  GSKYIYLEDLMRFM +DEASKTM
Sbjct: 601 DEQIKDTAHEDESTTQIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTM 660

Query: 661 SLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVI 720
            LFEGA ESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVI
Sbjct: 661 GLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVI 720

Query: 721 WLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQM 780
           WLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFE+IIFLFVMHPFDVGDRCEIDGVQM
Sbjct: 721 WLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQM 780

Query: 781 IVEEMNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAI 840
           IVEEMNILTT+FLR+DNQKIIFPNSVLATKAIHN+YRSPDMGD VEFCLHISTPPEKIAI
Sbjct: 781 IVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGVEFCLHISTPPEKIAI 840

Query: 841 MRQRILGFIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVE 900
           MRQRI+ +IEGKKEHWCPAP+I+LKDVEELNRMRIAIWLTHRMNHQDMGERWTRRA LVE
Sbjct: 841 MRQRIVSYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVE 900

Query: 901 ELVKIFQELDLQYRLLPLNINVCSLPPVNSTRLP 930
           ELVKIFQELDLQYRLLPL+INV SLPPVNST  P
Sbjct: 901 ELVKIFQELDLQYRLLPLDINVRSLPPVNSTNFP 921

BLAST of Cp4.1LG16g03410 vs. TAIR 10
Match: AT1G78610.1 (mechanosensitive channel of small conductance-like 6 )

HSP 1 Score: 968.4 bits (2502), Expect = 4.2e-282
Identity = 542/923 (58.72%), Postives = 677/923 (73.35%), Query Frame = 0

Query: 50  MAVSDASDRKEVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSKRNNGE- 109
           MAV DA+DR+EVIVK+D  + ++N       S  + G+IWR+ SYDFW D +   N G  
Sbjct: 1   MAV-DAADRREVIVKIDGENGNNNG-----VSGETVGKIWRDGSYDFWTDGEGNLNKGHN 60

Query: 110 ----------GSNGDGGKGEDFEFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEM 169
                      + G+  K E FEFR+     EDPP+KLIGQFLHKQ+ASGE+CLDMD+ M
Sbjct: 61  AAAVDSDRSAATTGEQQKDEGFEFRRG----EDPPTKLIGQFLHKQQASGEICLDMDLGM 120

Query: 170 LELPHDKTLPTVAESPMRRSSKELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQ 229
            EL   + L  V+ESP R S+K                         PV          +
Sbjct: 121 DEL-QSRGLTPVSESP-RVSTKR-----------------------DPVG---------R 180

Query: 230 RDHRPHGNGHDDGAAEVVRCTSNSSFERNVSFQRKSS-LLKTKTKSRLLDPPEHQ----- 289
           RD R + N +DDG  EVV+C+ N     N   QR SS LLK +T+SRL DPP  Q     
Sbjct: 181 RDSRSNTNNNDDG--EVVKCSGN-----NAPIQRSSSTLLKMRTRSRLSDPPTPQLPPQT 240

Query: 290 -DRRSGRVPKSGQIRSGLLSKAL----DEEDDDPFLEEDLPDEYKKANLGVLTMLQWASL 349
            D +SGR+PKSGQ++SG   K+     +EE+DDPF  EDLP+EY+K  L +  +L+W SL
Sbjct: 241 ADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSL 300

Query: 350 ILIIAALICTLTIRYWRRKKLWKLELWKWEVMILVLICGRLFSGWGIRIIVFFIERSFLL 409
           ILIIA  +CTL I   R+KKLW+L+LWKWE M+LVLICGRL S W ++I+VFFIER+FLL
Sbjct: 301 ILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLL 360

Query: 410 RKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSDALEYVTKVLICLLVSTL 469
           RKRVLYFVYGVRKAVQNCLWLGLVL+AW+FLFD+KV +   + AL  VTK+ +CLLV  L
Sbjct: 361 RKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCLLVGFL 420

Query: 470 VWLVKTLIVKVLASSFHVSTYFDRIQDALYNQYVIETLSGPPLIEIQKSKEEEEKLAEEV 529
           +WLVKTL+VKVLASSFH+STYFDRIQ++L+ QYVIETLSGPPLIEIQK++EEEE+++ EV
Sbjct: 421 LWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVEV 480

Query: 530 MKLQNAGATIPPDLRASAFSSPEGGRVIGSGGLQKSP--RGRSAKLSRALSKKG-----D 589
            K QN G                   V    G QKSP   G+S  LS  LS  G     +
Sbjct: 481 KKFQNPGG------------------VEIQSGAQKSPMKTGKSPFLSHVLSNGGGGGGEN 540

Query: 590 EGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQIKDSAHEDESTTQIKSEYEAK 649
           +GITID LH+L+PKNVSAW MKRLMNI+R+G+L+TLDEQ++D + +D+   QI+SE+EAK
Sbjct: 541 KGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFEAK 600

Query: 650 VAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFEGASESRKISKSSLKNWVVNA 709
           +AA++IF NVA  GSK+IY  D+MRF+ +DEA KT+SLFEGASE+ +ISKSSLKNWVVNA
Sbjct: 601 LAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNA 660

Query: 710 FRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLVA 769
           FRERRALALTLNDTKTAVN+LH+MVNI+V +IILVIWL+ILGI ++KFL+ ++SQ+V+VA
Sbjct: 661 FRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVA 720

Query: 770 FVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIIFPN 829
           F+FGN CK VFE+II+LFV+HPFDVGDRCEIDGVQM+VEEMNILTTVFLRFDNQK+++PN
Sbjct: 721 FIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPN 780

Query: 830 SVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQRILGFIEGKKEHWCPAPLIIL 889
           S+L TK+I NYYRSPDMGD +EF +HI+TP EKI +++QRI  +IEGKK+HW PAP+I+ 
Sbjct: 781 SLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVF 840

Query: 890 KDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVKIFQELDLQYRLLPLNINVCS 939
           KD+E LN +RIA+W THRMNHQDMGE+W RR+ LVEE+ KI +ELD++YRL PL+INV +
Sbjct: 841 KDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYPLDINVRN 853

BLAST of Cp4.1LG16g03410 vs. TAIR 10
Match: AT3G14810.1 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 921.8 bits (2381), Expect = 4.5e-268
Identity = 507/918 (55.23%), Postives = 664/918 (72.33%), Query Frame = 0

Query: 50  MAVSDASDRKEVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSKRN-NGE 109
           MA  D++DR++ IV ++    S    A    S    G IW+ESSYDFW+    K +  G+
Sbjct: 1   MAAVDSTDRRDFIVNIN-GQESGAVGATGSSSNAEGGNIWKESSYDFWDGEKGKNDKKGD 60

Query: 110 GSNGDGGKGEDFEFRQQRK------DVEDPPSKLIGQFLHKQKASG-EMCLDMDMEMLEL 169
             + DGG    F FRQ+ +      ++ DPPSKLIGQFLHKQ+ASG E+ LD+++ M EL
Sbjct: 61  DEDEDGG---SFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAEL 120

Query: 170 -PHDKTLPTVAESPMRRSSKELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRD 229
             +    P  A +  RR    +  S   +    ++D++RRR        ++R       D
Sbjct: 121 QSNTPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRR--------QNRTSLGGSSD 180

Query: 230 HRPHGNGHDDGAAEVVRCTSNSSFERNVSFQRKSSLLKTKTKSRLLDPP-------EHQD 289
                 G +   AEV++C S           +K  L + KTKSRL DPP       +  +
Sbjct: 181 E----EGRNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQDPPTPTHPAIDKTE 240

Query: 290 RRSGRVPKSGQIRSGLLSKA-----------LDEEDDDPFLEEDLPDEYKKANLGVLTML 349
            +SGR  +SG  +SG L K+            +EE++DPFL+EDLP+E+K+  L     L
Sbjct: 241 MKSGR--RSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKLSFWVFL 300

Query: 350 QWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMILVLICGRLFSGWGIRIIVFFIE 409
           +W SL+LI+ +L+C+LTI   +RK  WKL+LWKWEV +LVLICGRL S W +RIIVF +E
Sbjct: 301 EWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVE 360

Query: 410 RSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSDALEYVTKVLICL 469
           ++F  RKRVLYFVYGVRK+VQNCLWLGLVL+AW+FLFD KV+RE +S AL YVT+VL+CL
Sbjct: 361 KNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYVTRVLVCL 420

Query: 470 LVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQYVIETLSGPPLIEIQKSKEEEEK 529
           LV+ ++WLVKT++VKVLASSFH+STYFDRIQ++L+ QYVIETLSGPPL+EIQ+ +EEE++
Sbjct: 421 LVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEEQQ 480

Query: 530 LAEEVMKLQN-AGATIPPDLRASAFSSPEGGRVIGSGGLQKSPRGRSAKLSRALSKKGD- 589
           +AE+V  L+  AGA +PP L+A+  S  +               G+S  L+R  SK+G+ 
Sbjct: 481 VAEDVKSLEKLAGAKLPPALKATVKSFMK--------------VGKSPGLNRIGSKRGED 540

Query: 590 -EGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQIKDSAHEDESTTQIKSEYEA 649
            EGI ID L R++ KNVSAWNMKRLMNI+  GA+STLD+ ++D+  EDE  T I+SEYEA
Sbjct: 541 GEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSEYEA 600

Query: 650 KVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFEGASESRKISKSSLKNWVVN 709
           K AA++IF NV   GS+YIYLED +RF+ E+EA + M+LFEGASES KISKS LKNWVV 
Sbjct: 601 KCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVK 660

Query: 710 AFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLV 769
           AFRERRALALTLNDTKTAV++LHR++N+++ +II++IWLLILGIAT++FLL ++SQL+LV
Sbjct: 661 AFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLV 720

Query: 770 AFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIIFP 829
           AFVFGN+CKT+FEAIIFLFVMHPFDVGDRCEIDGVQ++VEEMNILTTVFLR+DNQKII+P
Sbjct: 721 AFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYP 780

Query: 830 NSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQRILGFIEGKKEHWCPAPLII 889
           NSVL TK I NYYRSPDMGDAVEFC+HI+TPPEKI  ++QRIL +++ KK++W PAP+I+
Sbjct: 781 NSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIV 840

Query: 890 LKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVKIFQELDLQYRLLPLNINVC 934
              +++LN ++IA+WLTHRMNHQDMGER+ RR  L+EE+ K  +ELD++YRL PLNINV 
Sbjct: 841 FLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNINVR 875

BLAST of Cp4.1LG16g03410 vs. TAIR 10
Match: AT1G53470.1 (mechanosensitive channel of small conductance-like 4 )

HSP 1 Score: 914.1 bits (2361), Expect = 9.4e-266
Identity = 521/926 (56.26%), Postives = 659/926 (71.17%), Query Frame = 0

Query: 50  MAVSDASDRKEVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFW-NDSDSKRNNGE 109
           MAV     R++ +V++D  D               S + WRESS +FW ND  SK   GE
Sbjct: 1   MAVDSTDQRRDFVVRIDGEDN------------GDSEKFWRESSINFWHNDKSSKPPGGE 60

Query: 110 GSNGDGGKGEDFEFRQQRKDVE-DPPSKLIGQFLHKQKASG-EMCLDMDMEMLELPHDKT 169
             +G      DF  R   K  E DPPSKLI QFL+KQKASG E+ LDM+  M EL    T
Sbjct: 61  EDDG----SFDFMRRSSEKSEEPDPPSKLINQFLNKQKASGDEISLDMEANMPEL-QKNT 120

Query: 170 LPTVAESPMRRSSKELKVSFESISEISESDSLRRRHR-----------DSPVDEEHRVQQ 229
           +P ++ + +  S+  +     +       D++RRR             DS  DEE+RV  
Sbjct: 121 VPPLSSTAVSGSASPVTAPVTASYRNGTGDAIRRRQNRVTLSPSVKDGDSSEDEENRV-- 180

Query: 230 PPQRDHRPHGNGHDDGAAEVVRCTSNSSFERNVSFQRKSSLLKTKTKSRLLDP--PEHQD 289
                         DG +EVV+CTSN       S  R  +L+K KT+SRL+DP  P + D
Sbjct: 181 --------------DG-SEVVKCTSNR------STMRTKTLMKMKTRSRLMDPPTPTYPD 240

Query: 290 RRSGRVPKSGQIRSGLL-------------SKALDEEDDDPFLEEDLPDEYKKANLGVLT 349
             SGR P+SG +  G               SK L EE++DPF EEDLP+  +K  + V  
Sbjct: 241 MVSGRTPRSGNLNPGFSGRNTKPGTPNQGGSKDL-EEEEDPFSEEDLPEGLRKEKICVWV 300

Query: 350 MLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMILVLICGRLFSGWGIRIIVFF 409
           +++W  LILIIA+LIC+L I Y R K LW L LWKWEVM+LVLICGRL S W +++ V+F
Sbjct: 301 IIEWIFLILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYF 360

Query: 410 IERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSDALEYVTKVLI 469
           +E +FL RK+VLYFVYG+RK VQNCLWLGLVLIAW+FLFD KV+RE++S  L+YVTKVLI
Sbjct: 361 VESNFLWRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLI 420

Query: 470 CLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQYVIETLSGPPLIEIQKSKEEE 529
           CLLV+ ++WL+KTL+VKVLASSFH+STYFDRIQ++L+ QYVIETLSGPP IEI     EE
Sbjct: 421 CLLVAVIIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEIH---IEE 480

Query: 530 EKLAEEVMKLQNAGATIPPDLRASAFSSPEGGRVIGSGGLQKSPR--GRSAKLSRALSKK 589
           EK+A +V   +  G  + P L   A SSP     +GSG LQKSP   G+S  LSR+ SKK
Sbjct: 481 EKVANDVKTFEIVGRKLSP-LGPKAVSSPP-QVTVGSGRLQKSPSRVGKSPVLSRSGSKK 540

Query: 590 --GDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQIKD-SAHEDESTTQIKS 649
             G+EGI IDHL R++ KNVSAW MK+LMN+++ G LSTLDEQI+D +  ED+  TQI+S
Sbjct: 541 EGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRS 600

Query: 650 EYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFEGASESRKISKSSLKN 709
           E+EAK+AA++IFQNVA  GS+YIY+ED MRF+ EDE+ + M LFEGASE  KISKS LKN
Sbjct: 601 EFEAKLAARKIFQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKN 660

Query: 710 WVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQ 769
           WVVNAFRERRALALTLNDTKTAVN+LHR+V++LVS++IL+IWLLILGIAT+KFLL ++SQ
Sbjct: 661 WVVNAFRERRALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQ 720

Query: 770 LVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQK 829
           L+LV FVFGN+CKT+FEA+IF+FVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQK
Sbjct: 721 LLLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQK 780

Query: 830 IIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQRILGFIEGKKEHWCPA 889
           I++PNS+L TK I NYYRSPDM DA+EF +HI+TPPEK   +RQRIL +++ KK+HW P+
Sbjct: 781 IVYPNSLLGTKPIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPS 840

Query: 890 PLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVKIFQELDLQYRLLPLN 939
           P+I+ +D+  LN ++IA+W TH+MNHQ+MGER+ RR  L+EE+ ++ +ELD++YRL PLN
Sbjct: 841 PMIVFRDMCGLNSVKIAMWPTHKMNHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLN 880

BLAST of Cp4.1LG16g03410 vs. TAIR 10
Match: AT3G14810.2 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 851.7 bits (2199), Expect = 5.7e-247
Identity = 482/918 (52.51%), Postives = 631/918 (68.74%), Query Frame = 0

Query: 50  MAVSDASDRKEVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSKRN-NGE 109
           MA  D++DR++ IV ++    S    A    S    G IW+ESSYDFW+    K +  G+
Sbjct: 1   MAAVDSTDRRDFIVNIN-GQESGAVGATGSSSNAEGGNIWKESSYDFWDGEKGKNDKKGD 60

Query: 110 GSNGDGGKGEDFEFRQQRK------DVEDPPSKLIGQFLHKQKASG-EMCLDMDMEMLEL 169
             + DGG    F FRQ+ +      ++ DPPSKLIGQFLHKQ+ASG E+ LD+++ M EL
Sbjct: 61  DEDEDGG---SFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAEL 120

Query: 170 -PHDKTLPTVAESPMRRSSKELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRD 229
             +    P  A +  RR    +  S   +    ++D++RRR        ++R       D
Sbjct: 121 QSNTPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRR--------QNRTSLGGSSD 180

Query: 230 HRPHGNGHDDGAAEVVRCTSNSSFERNVSFQRKSSLLKTKTKSRLLDPP-------EHQD 289
                 G +   AEV++C S           +K  L + KTKSRL DPP       +  +
Sbjct: 181 E----EGRNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQDPPTPTHPAIDKTE 240

Query: 290 RRSGRVPKSGQIRSGLLSKA-----------LDEEDDDPFLEEDLPDEYKKANLGVLTML 349
            +SGR  +SG  +SG L K+            +EE++DPFL+EDLP+E+K+  L     L
Sbjct: 241 MKSGR--RSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKLSFWVFL 300

Query: 350 QWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVMILVLICGRLFSGWGIRIIVFFIE 409
           +W SL+LI+ +L+C+LTI   +RK  WKL+LWKWEV +LVLICGRL S W +RIIVF +E
Sbjct: 301 EWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVE 360

Query: 410 RSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSDALEYVTKVLICL 469
           ++F  RKRVLYFVYGVRK+VQNCLWLGLVL+AW+FLFD KV+RE +S AL          
Sbjct: 361 KNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALR--------- 420

Query: 470 LVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQYVIETLSGPPLIEIQKSKEEEEK 529
                                   TYFDRIQ++L+ QYVIETLSGPPL+EIQ+ +EEE++
Sbjct: 421 ------------------------TYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEEQQ 480

Query: 530 LAEEVMKLQN-AGATIPPDLRASAFSSPEGGRVIGSGGLQKSPRGRSAKLSRALSKKGD- 589
           +AE+V  L+  AGA +PP L+A+  S  +               G+S  L+R  SK+G+ 
Sbjct: 481 VAEDVKSLEKLAGAKLPPALKATVKSFMK--------------VGKSPGLNRIGSKRGED 540

Query: 590 -EGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQIKDSAHEDESTTQIKSEYEA 649
            EGI ID L R++ KNVSAWNMKRLMNI+  GA+STLD+ ++D+  EDE  T I+SEYEA
Sbjct: 541 GEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSEYEA 600

Query: 650 KVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSLFEGASESRKISKSSLKNWVVN 709
           K AA++IF NV   GS+YIYLED +RF+ E+EA + M+LFEGASES KISKS LKNWV  
Sbjct: 601 KCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWV-- 660

Query: 710 AFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLV 769
           AFRERRALALTLNDTKTAV++LHR++N+++ +II++IWLLILGIAT++FLL ++SQL+LV
Sbjct: 661 AFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLV 720

Query: 770 AFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIIFP 829
           AFVFGN+CKT+FEAIIFLFVMHPFDVGDRCEIDGVQ++VEEMNILTTVFLR+DNQKII+P
Sbjct: 721 AFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYP 780

Query: 830 NSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMRQRILGFIEGKKEHWCPAPLII 889
           NSVL TK I NYYRSPDMGDAVEFC+HI+TPPEKI  ++QRIL +++ KK++W PAP+I+
Sbjct: 781 NSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIV 840

Query: 890 LKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEELVKIFQELDLQYRLLPLNINVC 934
              +++LN ++IA+WLTHRMNHQDMGER+ RR  L+EE+ K  +ELD++YRL PLNINV 
Sbjct: 841 FLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNINVR 840

BLAST of Cp4.1LG16g03410 vs. TAIR 10
Match: AT2G17000.1 (Mechanosensitive ion channel family protein )

HSP 1 Score: 801.6 bits (2069), Expect = 6.8e-232
Identity = 455/886 (51.35%), Postives = 611/886 (68.96%), Query Frame = 0

Query: 53  SDASDRKEVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSKRNNGEGSNG 112
           ++ + +K+ +  +D+ D         +    SSG  +  +SY FW D          +  
Sbjct: 23  NEKTKKKKKLANLDDGD---------IAKTQSSGSSFDGNSYKFWQDI---------ATD 82

Query: 113 DGGKGEDFEFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPHDKTLPTVAE 172
           D  K   F+F Q R+                     E+ LD++ E  E         V+ 
Sbjct: 83  DYTKSGSFDFPQYRE---------------------EITLDVNEETEE------TEDVSN 142

Query: 173 SPMRRSSKELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQRDHRPHGNGHDDGA 232
           +     SKE +V F+  S  + + S   R   S         +             +D  
Sbjct: 143 NNNLSGSKETRVFFKINSSGTNNMSGSVRSCTSSTSFSSATMRLNLEQQL------EDEG 202

Query: 233 AEVVRCTSNSSFERNVSFQRKSSLL-KTKTKSRLLDPPEHQDRR-SGRVPKSGQIRSGLL 292
             VVRC+S           RK+ L+ + K +SRL+DPP+ ++++ S  +  S Q+RSGLL
Sbjct: 203 EVVVRCSS----------VRKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLL 262

Query: 293 SKALD--EEDDDPFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKL 352
            +  D  EE+DD   EED+P EY+K  +  +T+LQW SLI ++ AL+ +L +  WR   L
Sbjct: 263 GRHSDDIEEEDDSSAEEDVPVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATL 322

Query: 353 WKLELWKWEVMILVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWL 412
           W L LWKWEV++LVLICGRL SG GIRIIVFFIER+FLLRKRVLYFVYGV+ AVQNCLWL
Sbjct: 323 WSLHLWKWEVVLLVLICGRLVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWL 382

Query: 413 GLVLIAWNFLFDDKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTY 472
           GLVL+AW+FLFD KV++E +SD L  ++K+L+C L+ST++WL+KTL+VKVLASSFHVSTY
Sbjct: 383 GLVLLAWHFLFDKKVEKETQSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTY 442

Query: 473 FDRIQDALYNQYVIETLSGPPLIEIQKSKEEEEKLAEEVMKLQNAGATIPPDLRASAFSS 532
           FDRIQ+AL++ Y+IETLSGPP++E+ + +EEE++  +E+ K+Q  GA + P+L ++AF  
Sbjct: 443 FDRIQEALFHHYLIETLSGPPMLELSRIEEEEDRTQDEIYKMQKGGADLSPELCSAAFPQ 502

Query: 533 PEGGRVIGSGGLQKSPRGRSAKLSRALSKKG-DEGITIDHLHRLSPKNVSAWNMKRLMNI 592
            + G  +            + K S  + K G D GIT+D LH+++ KNVSAWNMKRLM I
Sbjct: 503 EKSGSTM------------NMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKI 562

Query: 593 VRHGALSTLDEQIKDSAHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFM 652
           VR+ +LSTLDEQ   +  EDEST QI+SE EAK AA++IF+NVA  G+K+IYLEDLMRF+
Sbjct: 563 VRNVSLSTLDEQALQNTCEDESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFL 622

Query: 653 VEDEASKTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNI 712
             DEA KTM LFEGA  ++KI+KS+LKNW+VNAFRERRALALTLNDTKTAVNKLH M++ 
Sbjct: 623 RVDEAMKTMCLFEGALVTKKITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISF 682

Query: 713 LVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGD 772
           L +++I+VIWL++L IATSK+LLF+TSQ+VL+AF+FGN+ KTVFE+IIFLF++HP+DVGD
Sbjct: 683 LTAIVIIVIWLILLEIATSKYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGD 742

Query: 773 RCEIDGVQMIVEEMNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHI 832
           R  ID V+M+VEEMNILTTVFLR DN KI++PN +L  KAIHNY RSPDMGD V  C+HI
Sbjct: 743 RLLIDTVEMVVEEMNILTTVFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHI 802

Query: 833 STPPEKIAIMRQRILGFIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGER 892
           +TPPEKIA ++QRI  +I+ K E+W P   +I+KDVE+LN +RIAIWL H++NHQ+MGER
Sbjct: 803 TTPPEKIAAIKQRISSYIDSKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGER 835

Query: 893 WTRRAFLVEELVKIFQELDLQYRLLPLNINVCSLPPVNSTRLPATW 934
           +TRRA L+EE++KI  ELD+QYR  PL+INV ++P V S+R+P  W
Sbjct: 863 FTRRALLIEEVIKILLELDIQYRFHPLDINVKTMPTVVSSRVPPAW 835