Cp4.1LG16g02620 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG16g02620
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionCysteine protease
LocationCp4.1LG16: 4685027 .. 4687130 (+)
RNA-Seq ExpressionCp4.1LG16g02620
SyntenyCp4.1LG16g02620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCTCACGAAGCATCAGCATAAAATGGCCTGCTCTGATACCCATAATCACAGGTCTTCTTCTCCATTTTTTCTCCTTCAATTCTCAGCGGTGAAAATGGTGAAGGGTTTCTCTTTCCTCGTCGTTCTCTCTCTTATAGCCGCCACGGCGATTGGCAGCGAGGTCCTCTCCGGAGAATCGGAGGGAGATTTTATAATCCGTCAGGTTGTCGACGACGGAGGAGTCAACGGAGGAAGTAATGGCGACGATCTGCTACTCGGAGCCGAGCACCACTTTTCGATCTTTAAGCGAAGGTTTGGGAAGTCGTACGCCTCCAACGAGGAGCATGATCACCGGTTCAGGATCTTTAAAGCTAACCTGAGGCGAGCTCGACGCCACCAGGCTCTTGATTCCTCTGCTACTCATGGCGTCACTCAGTTTTCTGATTTGACTCCATCGGAGTTCAGGAGATCGTTTCTAGGGCTTAGAGATCGTCTTCGTCTCCCTGCGGATGCTAACAAGGCCCCTATTCTTCCTACGGATGATCTCCCAACCGATTTCGATTGGAGAGATCACGGCGCTGTCACGGAAGTCAAGAATCAGGTTTGTGTTTTTCTTTTACCATATTTTTCCATCTTCGGCTATTTCGTTTACGTTAGCTATTTTTTAGTCGGTACTCAACTAAGGTTCTGATTTTTGTGATTGTTTTTGTTTTTATTTTCTCAGGGTTCGTGTGGATCGTGCTGGAGTTTCAGTACAACCGGTGCTCTTGAAGGCGCTAACTTCCTTGCTACTGGAAAACTTGTTAGCCTGAGCGAACAACAGCTCGTAGACTGTGATCACGAGGTTTGGTTTTAATTTTCACTTGTCTTCGATATTCTGTTCTTTGGTGAAATTTACAGCTTAACGTGTAGATGAAGACCTAATTACACGTTTGTTAATCATGGTTGCCCTCGATAAGATGCTACAAATTTGTTTATTACTCCTGTTCTATGCTATGAGACAAGTCATAAATCTTTGCGTTCATCAAGTATTGGCTGAAAGTACTTGACAAATAATTAAGTTTTAAAGCATTTCTATACTTTTTCCAGTGTGATCCAGAAGAAAAAGACTCCTGTGATTCTGGCTGCAATGGAGGGCTAATGAACAGTGCATTCGAGTATACACTGAAAGCCGGTGGACTTATGCAAGAGAAAGACTACCCATACACTGGTACAGATCGTGGAACCTGCAAATTTGACAAGAGCAAAATTGCTGCATCAGTGGCCAACTTCAGTGTTGTATCCCTTGATGAAGAACAAATTGCTGCCAATCTCTTGAAGAATGGCCCTCTTGCAGGTAATTTCATTCAACCAAGCTTTTCTTGAATCCTATTACTTTCTCTAATCAATCAGGGTTCCTGACTGCTCAAATTCTCTTAAATGCAGTTGCTATCAACGCCGTATACATGCAGACATATGTAGGAGGCGTTTCTTGCCCATACATTTGCTCAAAGCGCTTGGATCACGGAGTTTTGTTGGTTGGTTATGGGTCTGCTGGCTATGCTCCCATCCGAATGAAAGAGAAGCCTTACTGGATCATTAAGAACTCGTGGGGAGCGAAATGGGGGGAGAATGGATTCTACAAAATCTGCAGAGGTCGCAACGTCTGTGGGGTCGATTCCATGGTATCGACAGTGGCCGCAGTTCATACCGCCGCAAACTAGTTTATGGAGAATTACTCTCACAGCTACAATTCGTGTATATATTACTGCAACATTATCACTGCCATTTCTAGCATTGGTGGGAGGGATGTCTCTCTAAAAGTAGAATTTAAATTCTCTGGTTTATCTAATGCATAGTTATATTTAGTTCTTCAAGCTATTATGGAGAAGGGATTGCTTGTAAGCAAAAAAAACAATCATCTGTCAATGAATTGGGGTTGGTTGGCTCACCTGCTCAACTTGATAGAGATGCCAATGAAAACTTGTCCCAATGTAAATGTCAACAATGATAGCCGAAAGATGCTTTCTTGATGTGTCATCTTTATCTTTTCAGATTCTAAACGTCAACATGATAATGACAATGCTTATTAAAGAAATCCATTTGGAACCATTTTCTGTTAAGGTTTCAATCGTACCGAATCG

mRNA sequence

CTCCTCACGAAGCATCAGCATAAAATGGCCTGCTCTGATACCCATAATCACAGGTCTTCTTCTCCATTTTTTCTCCTTCAATTCTCAGCGGTGAAAATGGTGAAGGGTTTCTCTTTCCTCGTCGTTCTCTCTCTTATAGCCGCCACGGCGATTGGCAGCGAGGTCCTCTCCGGAGAATCGGAGGGAGATTTTATAATCCGTCAGGTTGTCGACGACGGAGGAGTCAACGGAGGAAGTAATGGCGACGATCTGCTACTCGGAGCCGAGCACCACTTTTCGATCTTTAAGCGAAGGTTTGGGAAGTCGTACGCCTCCAACGAGGAGCATGATCACCGGTTCAGGATCTTTAAAGCTAACCTGAGGCGAGCTCGACGCCACCAGGCTCTTGATTCCTCTGCTACTCATGGCGTCACTCAGTTTTCTGATTTGACTCCATCGGAGTTCAGGAGATCGTTTCTAGGGCTTAGAGATCGTCTTCGTCTCCCTGCGGATGCTAACAAGGCCCCTATTCTTCCTACGGATGATCTCCCAACCGATTTCGATTGGAGAGATCACGGCGCTGTCACGGAAGTCAAGAATCAGGGTTCGTGTGGATCGTGCTGGAGTTTCAGTACAACCGGTGCTCTTGAAGGCGCTAACTTCCTTGCTACTGGAAAACTTGTTAGCCTGAGCGAACAACAGCTCGTAGACTGTGATCACGAGTGTGATCCAGAAGAAAAAGACTCCTGTGATTCTGGCTGCAATGGAGGGCTAATGAACAGTGCATTCGAGTATACACTGAAAGCCGGTGGACTTATGCAAGAGAAAGACTACCCATACACTGGTACAGATCGTGGAACCTGCAAATTTGACAAGAGCAAAATTGCTGCATCAGTGGCCAACTTCAGTGTTGTATCCCTTGATGAAGAACAAATTGCTGCCAATCTCTTGAAGAATGGCCCTCTTGCAGTTGCTATCAACGCCGTATACATGCAGACATATGTAGGAGGCGTTTCTTGCCCATACATTTGCTCAAAGCGCTTGGATCACGGAGTTTTGTTGGTTGGTTATGGGTCTGCTGGCTATGCTCCCATCCGAATGAAAGAGAAGCCTTACTGGATCATTAAGAACTCGTGGGGAGCGAAATGGGGGGAGAATGGATTCTACAAAATCTGCAGAGGTCGCAACGTCTGTGGGGTCGATTCCATGGTATCGACAGTGGCCGCAGTTCATACCGCCGCAAACTAGTTTATGGAGAATTACTCTCACAGCTACAATTCGTGTATATATTACTGCAACATTATCACTGCCATTTCTAGCATTGGTGGGAGGGATGTCTCTCTAAAAGTAGAATTTAAATTCTCTGGTTTATCTAATGCATAGTTATATTTAGTTCTTCAAGCTATTATGGAGAAGGGATTGCTTGTAAGCAAAAAAAACAATCATCTGTCAATGAATTGGGGTTGGTTGGCTCACCTGCTCAACTTGATAGAGATGCCAATGAAAACTTGTCCCAATGTAAATGTCAACAATGATAGCCGAAAGATGCTTTCTTGATGTGTCATCTTTATCTTTTCAGATTCTAAACGTCAACATGATAATGACAATGCTTATTAAAGAAATCCATTTGGAACCATTTTCTGTTAAGGTTTCAATCGTACCGAATCG

Coding sequence (CDS)

ATGGCCTGCTCTGATACCCATAATCACAGGTCTTCTTCTCCATTTTTTCTCCTTCAATTCTCAGCGGTGAAAATGGTGAAGGGTTTCTCTTTCCTCGTCGTTCTCTCTCTTATAGCCGCCACGGCGATTGGCAGCGAGGTCCTCTCCGGAGAATCGGAGGGAGATTTTATAATCCGTCAGGTTGTCGACGACGGAGGAGTCAACGGAGGAAGTAATGGCGACGATCTGCTACTCGGAGCCGAGCACCACTTTTCGATCTTTAAGCGAAGGTTTGGGAAGTCGTACGCCTCCAACGAGGAGCATGATCACCGGTTCAGGATCTTTAAAGCTAACCTGAGGCGAGCTCGACGCCACCAGGCTCTTGATTCCTCTGCTACTCATGGCGTCACTCAGTTTTCTGATTTGACTCCATCGGAGTTCAGGAGATCGTTTCTAGGGCTTAGAGATCGTCTTCGTCTCCCTGCGGATGCTAACAAGGCCCCTATTCTTCCTACGGATGATCTCCCAACCGATTTCGATTGGAGAGATCACGGCGCTGTCACGGAAGTCAAGAATCAGGGTTCGTGTGGATCGTGCTGGAGTTTCAGTACAACCGGTGCTCTTGAAGGCGCTAACTTCCTTGCTACTGGAAAACTTGTTAGCCTGAGCGAACAACAGCTCGTAGACTGTGATCACGAGTGTGATCCAGAAGAAAAAGACTCCTGTGATTCTGGCTGCAATGGAGGGCTAATGAACAGTGCATTCGAGTATACACTGAAAGCCGGTGGACTTATGCAAGAGAAAGACTACCCATACACTGGTACAGATCGTGGAACCTGCAAATTTGACAAGAGCAAAATTGCTGCATCAGTGGCCAACTTCAGTGTTGTATCCCTTGATGAAGAACAAATTGCTGCCAATCTCTTGAAGAATGGCCCTCTTGCAGTTGCTATCAACGCCGTATACATGCAGACATATGTAGGAGGCGTTTCTTGCCCATACATTTGCTCAAAGCGCTTGGATCACGGAGTTTTGTTGGTTGGTTATGGGTCTGCTGGCTATGCTCCCATCCGAATGAAAGAGAAGCCTTACTGGATCATTAAGAACTCGTGGGGAGCGAAATGGGGGGAGAATGGATTCTACAAAATCTGCAGAGGTCGCAACGTCTGTGGGGTCGATTCCATGGTATCGACAGTGGCCGCAGTTCATACCGCCGCAAACTAG

Protein sequence

MACSDTHNHRSSSPFFLLQFSAVKMVKGFSFLVVLSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHFSIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDSSATHGVTQFSDLTPSEFRRSFLGLRDRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGANFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYPYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVSCPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRGRNVCGVDSMVSTVAAVHTAAN
Homology
BLAST of Cp4.1LG16g02620 vs. ExPASy Swiss-Prot
Match: P43296 (Cysteine protease RD19A OS=Arabidopsis thaliana OX=3702 GN=RD19A PE=1 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 8.1e-163
Identity = 284/376 (75.53%), Postives = 318/376 (84.57%), Query Frame = 0

Query: 26  VKGFSFLVVLSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHFS 85
           +K +  + VLS    +   S+V  G+   D +IRQVV      GG+  +  +L +E HFS
Sbjct: 4   LKLYFSVFVLSFFIVSVSSSDVNDGD---DLVIRQVV------GGA--EPQVLTSEDHFS 63

Query: 86  IFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDSSATHGVTQFSDLTPSEFRRSFL 145
           +FKR+FGK YASNEEHD+RF +FKANLRRARRHQ LD SATHGVTQFSDLT SEFR+  L
Sbjct: 64  LFKRKFGKVYASNEEHDYRFSVFKANLRRARRHQKLDPSATHGVTQFSDLTRSEFRKKHL 123

Query: 146 GLRDRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGAN 205
           G+R   +LP DANKAPILPT++LP DFDWRDHGAVT VKNQGSCGSCWSFS TGALEGAN
Sbjct: 124 GVRSGFKLPKDANKAPILPTENLPEDFDWRDHGAVTPVKNQGSCGSCWSFSATGALEGAN 183

Query: 206 FLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYPY 265
           FLATGKLVSLSEQQLVDCDHECDPEE DSCDSGCNGGLMNSAFEYTLK GGLM+E+DYPY
Sbjct: 184 FLATGKLVSLSEQQLVDCDHECDPEEADSCDSGCNGGLMNSAFEYTLKTGGLMKEEDYPY 243

Query: 266 TGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVSC 325
           TG D  TCK DKSKI ASV+NFSV+S+DEEQIAANL+KNGPLAVAINA YMQTY+GGVSC
Sbjct: 244 TGKDGKTCKLDKSKIVASVSNFSVISIDEEQIAANLVKNGPLAVAINAGYMQTYIGGVSC 303

Query: 326 PYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRGRNVCGV 385
           PYIC++RL+HGVLLVGYG+AGYAP R KEKPYWIIKNSWG  WGENGFYKIC+GRN+CGV
Sbjct: 304 PYICTRRLNHGVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGENGFYKICKGRNICGV 363

Query: 386 DSMVSTVAA-VHTAAN 401
           DSMVSTVAA V T A+
Sbjct: 364 DSMVSTVAATVSTTAH 368

BLAST of Cp4.1LG16g02620 vs. ExPASy Swiss-Prot
Match: Q9SUL1 (Probable cysteine protease RD19C OS=Arabidopsis thaliana OX=3702 GN=RD19C PE=2 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 5.3e-154
Identity = 266/374 (71.12%), Postives = 313/374 (83.69%), Query Frame = 0

Query: 29  FSFLVVLSLIAATAIGSEVLSGESEGDFI--IRQVVDDGGVNGGSNGDDLLLGAEHHFSI 88
           F FL+  +L+A  ++GS V+SGE    F+  IRQVV +         D+ LL AEHHF++
Sbjct: 6   FFFLIAATLLAG-SLGSTVISGEVTDGFVNPIRQVVPE-------ENDEQLLNAEHHFTL 65

Query: 89  FKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDSSATHGVTQFSDLTPSEFRRSFLG 148
           FK ++ K+YA+  EHDHRFR+FKANLRRARR+Q LD SA HGVTQFSDLTP EFRR FLG
Sbjct: 66  FKSKYEKTYATQVEHDHRFRVFKANLRRARRNQLLDPSAVHGVTQFSDLTPKEFRRKFLG 125

Query: 149 LRDR-LRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGAN 208
           L+ R  RLP D   APILPT DLPT+FDWR+ GAVT VKNQG CGSCWSFS  GALEGA+
Sbjct: 126 LKRRGFRLPTDTQTAPILPTSDLPTEFDWREQGAVTPVKNQGMCGSCWSFSAIGALEGAH 185

Query: 209 FLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYPY 268
           FLAT +LVSLSEQQLVDCDHECDP + +SCDSGC+GGLMN+AFEY LKAGGLM+E+DYPY
Sbjct: 186 FLATKELVSLSEQQLVDCDHECDPAQANSCDSGCSGGLMNNAFEYALKAGGLMKEEDYPY 245

Query: 269 TGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVSC 328
           TG D   CKFDKSKI ASV+NFSVVS DE+QIAANL+++GPLA+AINA++MQTY+GGVSC
Sbjct: 246 TGRDHTACKFDKSKIVASVSNFSVVSSDEDQIAANLVQHGPLAIAINAMWMQTYIGGVSC 305

Query: 329 PYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRG-RNVCG 388
           PY+CSK  DHGVLLVG+GS+GYAPIR+KEKPYWIIKNSWGA WGE+G+YKICRG  N+CG
Sbjct: 306 PYVCSKSQDHGVLLVGFGSSGYAPIRLKEKPYWIIKNSWGAMWGEHGYYKICRGPHNMCG 365

Query: 389 VDSMVSTVAAVHTA 399
           +D+MVSTVAAVHT+
Sbjct: 366 MDTMVSTVAAVHTS 371

BLAST of Cp4.1LG16g02620 vs. ExPASy Swiss-Prot
Match: P43295 (Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 SV=2)

HSP 1 Score: 545.0 bits (1403), Expect = 6.9e-154
Identity = 262/366 (71.58%), Postives = 304/366 (83.06%), Query Frame = 0

Query: 29  FSFLVVLSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHFSIFK 88
           FS  ++   ++ +  G E        D +IRQVVD+         +  +L +E HF++FK
Sbjct: 9   FSVSLIFVFVSVSVCGDE--------DVLIRQVVDE--------TEPKVLSSEDHFTLFK 68

Query: 89  RRFGKSYASNEEHDHRFRIFKANLRRARRHQALDSSATHGVTQFSDLTPSEFRRSFLGLR 148
           ++FGK Y S EEH +RF +FKANL RA RHQ +D SA HGVTQFSDLT SEFRR  LG++
Sbjct: 69  KKFGKVYGSIEEHYYRFSVFKANLLRAMRHQKMDPSARHGVTQFSDLTRSEFRRKHLGVK 128

Query: 149 DRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGANFLA 208
              +LP DAN+APILPT +LP +FDWRD GAVT VKNQGSCGSCWSFSTTGALEGA+FLA
Sbjct: 129 GGFKLPKDANQAPILPTQNLPEEFDWRDRGAVTPVKNQGSCGSCWSFSTTGALEGAHFLA 188

Query: 209 TGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYPYTGT 268
           TGKLVSLSEQQLVDCDHECDPEE+ SCDSGCNGGLMNSAFEYTLK GGLM+EKDYPYTGT
Sbjct: 189 TGKLVSLSEQQLVDCDHECDPEEEGSCDSGCNGGLMNSAFEYTLKTGGLMREKDYPYTGT 248

Query: 269 DRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVSCPYI 328
           D G+CK D+SKI ASV+NFSVVS++E+QIAANL+KNGPLAVAINA YMQTY+GGVSCPYI
Sbjct: 249 DGGSCKLDRSKIVASVSNFSVVSINEDQIAANLIKNGPLAVAINAAYMQTYIGGVSCPYI 308

Query: 329 CSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRGRNVCGVDSM 388
           CS+RL+HGVLLVGYGSAG++  R+KEKPYWIIKNSWG  WGENGFYKIC+GRN+CGVDS+
Sbjct: 309 CSRRLNHGVLLVGYGSAGFSQARLKEKPYWIIKNSWGESWGENGFYKICKGRNICGVDSL 358

Query: 389 VSTVAA 395
           VSTVAA
Sbjct: 369 VSTVAA 358

BLAST of Cp4.1LG16g02620 vs. ExPASy Swiss-Prot
Match: P25804 (Cysteine proteinase 15A OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 9.3e-151
Identity = 263/368 (71.47%), Postives = 304/368 (82.61%), Query Frame = 0

Query: 31  FLVVLSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHFSIFKRR 90
           FL  L L AA A  + V    +  DFIIRQVVD        N +D LL AEHHF+ FK +
Sbjct: 5   FLFALFLFAAVA--TAVTDDTNNDDFIIRQVVD--------NEEDHLLNAEHHFTSFKSK 64

Query: 91  FGKSYASNEEHDHRFRIFKANLRRARRHQALDSSATHGVTQFSDLTPSEFRRSFLGLRDR 150
           F KSYA+ EEHD+RF +FK+NL +A+ HQ  D +A HG+T+FSDLT SEFRR FLGL+ R
Sbjct: 65  FSKSYATKEEHDYRFGVFKSNLIKAKLHQNRDPTAEHGITKFSDLTASEFRRQFLGLKKR 124

Query: 151 LRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGANFLATG 210
           LRLPA A KAPILPT +LP DFDWR+ GAVT VK+QGSCGSCW+FSTTGALEGA++LATG
Sbjct: 125 LRLPAHAQKAPILPTTNLPEDFDWREKGAVTPVKDQGSCGSCWAFSTTGALEGAHYLATG 184

Query: 211 KLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYPYTGTDR 270
           KLVSLSEQQLVDCDH CDPE+  SCDSGCNGGLMN+AFEY L++GG++QEKDY YTG D 
Sbjct: 185 KLVSLSEQQLVDCDHVCDPEQAGSCDSGCNGGLMNNAFEYLLESGGVVQEKDYAYTGRD- 244

Query: 271 GTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVSCPYICS 330
           G+CKFDKSK+ ASV+NFSVV+LDE+QIAANL+KNGPLAVAINA +MQTY+ GVSCPY+C+
Sbjct: 245 GSCKFDKSKVVASVSNFSVVTLDEDQIAANLVKNGPLAVAINAAWMQTYMSGVSCPYVCA 304

Query: 331 K-RLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRGRNVCGVDSMV 390
           K RLDHGVLLVG+G   YAPIR+KEKPYWIIKNSWG  WGE G+YKICRGRNVCGVDSMV
Sbjct: 305 KSRLDHGVLLVGFGKGAYAPIRLKEKPYWIIKNSWGQNWGEQGYYKICRGRNVCGVDSMV 361

Query: 391 STVAAVHT 398
           STVAA  +
Sbjct: 365 STVAAAQS 361

BLAST of Cp4.1LG16g02620 vs. ExPASy Swiss-Prot
Match: Q10716 (Cysteine proteinase 1 OS=Zea mays OX=4577 GN=CCP1 PE=2 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 5.5e-143
Identity = 255/375 (68.00%), Postives = 294/375 (78.40%), Query Frame = 0

Query: 32  LVVLSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHFSIFKRRF 91
           L++LSL +A A+ + V       D +IRQVV       G + +DL L AE HF  F +RF
Sbjct: 6   LLLLSLASAAAVAAAV----DAEDPLIRQVVP------GGDDNDLELNAESHFLSFVQRF 65

Query: 92  GKSYASNEEHDHRFRIFKANLRRARRHQALDSSATHGVTQFSDLTPSEFRRSFLGLRDRL 151
           GKSY   +EH +R  +FK NLRRARRHQ LD SA HGVT+FSDLTP+EFRR++LGLR   
Sbjct: 66  GKSYKDADEHAYRLSVFKDNLRRARRHQLLDPSAEHGVTKFSDLTPAEFRRTYLGLRKSR 125

Query: 152 R-----LPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGANF 211
           R     L   A++AP+LPTD LP DFDWRDHGAV  VKNQGSCGSCWSFS +GALEGA++
Sbjct: 126 RALLRELGESAHEAPVLPTDGLPDDFDWRDHGAVGPVKNQGSCGSCWSFSASGALEGAHY 185

Query: 212 LATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYPYT 271
           LATGKL  LSEQQ VDCDHECD  E DSCDSGCNGGLM +AF Y  KAGGL  EKDYPYT
Sbjct: 186 LATGKLEVLSEQQFVDCDHECDSSEPDSCDSGCNGGLMTTAFSYLQKAGGLESEKDYPYT 245

Query: 272 GTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVSCP 331
           G+D G CKFDKSKI ASV NFSVVS+DE QI+ANL+K+GPLA+ INA YMQTY+GGVSCP
Sbjct: 246 GSD-GKCKFDKSKIVASVQNFSVVSVDEAQISANLIKHGPLAIGINAAYMQTYIGGVSCP 305

Query: 332 YICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRG---RNVC 391
           YIC + LDHGVLLVGYG++G+APIR+K+KPYWIIKNSWG  WGENG+YKICRG   RN C
Sbjct: 306 YICGRHLDHGVLLVGYGASGFAPIRLKDKPYWIIKNSWGENWGENGYYKICRGSNVRNKC 365

Query: 392 GVDSMVSTVAAVHTA 399
           GVDSMVSTV+AVH +
Sbjct: 366 GVDSMVSTVSAVHAS 369

BLAST of Cp4.1LG16g02620 vs. NCBI nr
Match: XP_023511874.1 (cysteine protease RD19A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 762 bits (1968), Expect = 1.42e-277
Identity = 376/376 (100.00%), Postives = 376/376 (100.00%), Query Frame = 0

Query: 25  MVKGFSFLVVLSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHF 84
           MVKGFSFLVVLSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHF
Sbjct: 1   MVKGFSFLVVLSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHF 60

Query: 85  SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDSSATHGVTQFSDLTPSEFRRSF 144
           SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDSSATHGVTQFSDLTPSEFRRSF
Sbjct: 61  SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDSSATHGVTQFSDLTPSEFRRSF 120

Query: 145 LGLRDRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGA 204
           LGLRDRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGA
Sbjct: 121 LGLRDRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGA 180

Query: 205 NFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYP 264
           NFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYP
Sbjct: 181 NFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYP 240

Query: 265 YTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVS 324
           YTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVS
Sbjct: 241 YTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVS 300

Query: 325 CPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRGRNVCG 384
           CPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRGRNVCG
Sbjct: 301 CPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRGRNVCG 360

Query: 385 VDSMVSTVAAVHTAAN 400
           VDSMVSTVAAVHTAAN
Sbjct: 361 VDSMVSTVAAVHTAAN 376

BLAST of Cp4.1LG16g02620 vs. NCBI nr
Match: KAG6571085.1 (Cysteine protease RD19A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 756 bits (1952), Expect = 3.90e-275
Identity = 371/376 (98.67%), Postives = 374/376 (99.47%), Query Frame = 0

Query: 25  MVKGFSFLVVLSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHF 84
           MVKGFSFLVVLSL+ ATAIGSEVLSGES+GDFIIRQVVDDGGVNGGSNGDDLLLGAEHHF
Sbjct: 1   MVKGFSFLVVLSLVVATAIGSEVLSGESDGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHF 60

Query: 85  SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDSSATHGVTQFSDLTPSEFRRSF 144
           SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALD SATHGVTQFSDLTPSEFRRSF
Sbjct: 61  SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDPSATHGVTQFSDLTPSEFRRSF 120

Query: 145 LGLRDRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGA 204
           LGLRDRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGA
Sbjct: 121 LGLRDRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGA 180

Query: 205 NFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYP 264
           NFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYP
Sbjct: 181 NFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYP 240

Query: 265 YTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVS 324
           YTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVS
Sbjct: 241 YTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVS 300

Query: 325 CPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRGRNVCG 384
           CPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENG+YKICRGRNVCG
Sbjct: 301 CPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGYYKICRGRNVCG 360

Query: 385 VDSMVSTVAAVHTAAN 400
           VDSMVSTVAAVHTAAN
Sbjct: 361 VDSMVSTVAAVHTAAN 376

BLAST of Cp4.1LG16g02620 vs. NCBI nr
Match: XP_022944124.1 (cysteine protease RD19A-like [Cucurbita moschata] >KAG7010902.1 Cysteine protease RD19A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 753 bits (1944), Expect = 6.46e-274
Identity = 370/376 (98.40%), Postives = 373/376 (99.20%), Query Frame = 0

Query: 25  MVKGFSFLVVLSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHF 84
           MVKGFSFLVVLSL+ ATAIGSEVLSGES+GDFIIRQVVDDGGVN GSNGDDLLLGAEHHF
Sbjct: 1   MVKGFSFLVVLSLVVATAIGSEVLSGESDGDFIIRQVVDDGGVNRGSNGDDLLLGAEHHF 60

Query: 85  SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDSSATHGVTQFSDLTPSEFRRSF 144
           SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALD SATHGVTQFSDLTPSEFRRSF
Sbjct: 61  SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDPSATHGVTQFSDLTPSEFRRSF 120

Query: 145 LGLRDRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGA 204
           LGLRDRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGA
Sbjct: 121 LGLRDRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGA 180

Query: 205 NFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYP 264
           NFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYP
Sbjct: 181 NFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYP 240

Query: 265 YTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVS 324
           YTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVS
Sbjct: 241 YTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVS 300

Query: 325 CPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRGRNVCG 384
           CPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENG+YKICRGRNVCG
Sbjct: 301 CPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGYYKICRGRNVCG 360

Query: 385 VDSMVSTVAAVHTAAN 400
           VDSMVSTVAAVHTAAN
Sbjct: 361 VDSMVSTVAAVHTAAN 376

BLAST of Cp4.1LG16g02620 vs. NCBI nr
Match: XP_022986555.1 (cysteine protease RD19A-like [Cucurbita maxima])

HSP 1 Score: 749 bits (1933), Expect = 3.07e-272
Identity = 369/376 (98.14%), Postives = 370/376 (98.40%), Query Frame = 0

Query: 25  MVKGFSFLVVLSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHF 84
           MV G S  V LSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHF
Sbjct: 1   MVMGLSLFVALSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHF 60

Query: 85  SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDSSATHGVTQFSDLTPSEFRRSF 144
           SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALD SA+HGVTQFSDLTPSEFRRSF
Sbjct: 61  SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDPSASHGVTQFSDLTPSEFRRSF 120

Query: 145 LGLRDRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGA 204
           LGLRDRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGA
Sbjct: 121 LGLRDRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGA 180

Query: 205 NFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYP 264
           NFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYP
Sbjct: 181 NFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYP 240

Query: 265 YTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVS 324
           YTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVS
Sbjct: 241 YTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVS 300

Query: 325 CPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRGRNVCG 384
           CPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRGRNVCG
Sbjct: 301 CPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRGRNVCG 360

Query: 385 VDSMVSTVAAVHTAAN 400
           VDSMVSTVAAVHTAAN
Sbjct: 361 VDSMVSTVAAVHTAAN 376

BLAST of Cp4.1LG16g02620 vs. NCBI nr
Match: ARA15252.1 (cysteine proteinase 2 [Citrullus lanatus])

HSP 1 Score: 708 bits (1827), Expect = 4.54e-256
Identity = 343/377 (90.98%), Postives = 361/377 (95.76%), Query Frame = 0

Query: 25  MVKGFSFLVVLSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHF 84
           MVK FS +V LSL+A +AIG+EV SGES+GDF+IRQVVDDGGVNGGSNGDDLLLGAEHHF
Sbjct: 1   MVKCFSLIVALSLLAVSAIGAEVFSGESDGDFVIRQVVDDGGVNGGSNGDDLLLGAEHHF 60

Query: 85  SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDSSATHGVTQFSDLTPSEFRRSF 144
           S+FKR+FGKSYAS EEHDHRFR+FKANLRRA+RHQALD SATHGVTQFSDLTPSEFRRSF
Sbjct: 61  SLFKRKFGKSYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120

Query: 145 LGLRDR-LRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG 204
           LGLR R L LPADANKAPILPTD LPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG
Sbjct: 121 LGLRGRRLGLPADANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG 180

Query: 205 ANFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDY 264
           ANFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLM+E DY
Sbjct: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMKENDY 240

Query: 265 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGV 324
           PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANL+KNGPLA+AINAV+MQTY+GGV
Sbjct: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGV 300

Query: 325 SCPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRGRNVC 384
           SCPYICSK LDHGVLLVGYGS  YAPIR+KEKPYWIIKNSWGA WGENG+YKICRGRN+C
Sbjct: 301 SCPYICSKHLDHGVLLVGYGSGAYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNIC 360

Query: 385 GVDSMVSTVAAVHTAAN 400
           GVDSMVSTVAAVHTAAN
Sbjct: 361 GVDSMVSTVAAVHTAAN 377

BLAST of Cp4.1LG16g02620 vs. ExPASy TrEMBL
Match: A0A6J1FW38 (cysteine protease RD19A-like OS=Cucurbita moschata OX=3662 GN=LOC111448673 PE=3 SV=1)

HSP 1 Score: 753 bits (1944), Expect = 3.13e-274
Identity = 370/376 (98.40%), Postives = 373/376 (99.20%), Query Frame = 0

Query: 25  MVKGFSFLVVLSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHF 84
           MVKGFSFLVVLSL+ ATAIGSEVLSGES+GDFIIRQVVDDGGVN GSNGDDLLLGAEHHF
Sbjct: 1   MVKGFSFLVVLSLVVATAIGSEVLSGESDGDFIIRQVVDDGGVNRGSNGDDLLLGAEHHF 60

Query: 85  SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDSSATHGVTQFSDLTPSEFRRSF 144
           SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALD SATHGVTQFSDLTPSEFRRSF
Sbjct: 61  SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDPSATHGVTQFSDLTPSEFRRSF 120

Query: 145 LGLRDRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGA 204
           LGLRDRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGA
Sbjct: 121 LGLRDRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGA 180

Query: 205 NFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYP 264
           NFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYP
Sbjct: 181 NFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYP 240

Query: 265 YTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVS 324
           YTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVS
Sbjct: 241 YTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVS 300

Query: 325 CPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRGRNVCG 384
           CPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENG+YKICRGRNVCG
Sbjct: 301 CPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGYYKICRGRNVCG 360

Query: 385 VDSMVSTVAAVHTAAN 400
           VDSMVSTVAAVHTAAN
Sbjct: 361 VDSMVSTVAAVHTAAN 376

BLAST of Cp4.1LG16g02620 vs. ExPASy TrEMBL
Match: A0A6J1JED6 (cysteine protease RD19A-like OS=Cucurbita maxima OX=3661 GN=LOC111484259 PE=3 SV=1)

HSP 1 Score: 749 bits (1933), Expect = 1.49e-272
Identity = 369/376 (98.14%), Postives = 370/376 (98.40%), Query Frame = 0

Query: 25  MVKGFSFLVVLSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHF 84
           MV G S  V LSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHF
Sbjct: 1   MVMGLSLFVALSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHF 60

Query: 85  SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDSSATHGVTQFSDLTPSEFRRSF 144
           SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALD SA+HGVTQFSDLTPSEFRRSF
Sbjct: 61  SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDPSASHGVTQFSDLTPSEFRRSF 120

Query: 145 LGLRDRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGA 204
           LGLRDRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGA
Sbjct: 121 LGLRDRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGA 180

Query: 205 NFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYP 264
           NFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYP
Sbjct: 181 NFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYP 240

Query: 265 YTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVS 324
           YTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVS
Sbjct: 241 YTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVS 300

Query: 325 CPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRGRNVCG 384
           CPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRGRNVCG
Sbjct: 301 CPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRGRNVCG 360

Query: 385 VDSMVSTVAAVHTAAN 400
           VDSMVSTVAAVHTAAN
Sbjct: 361 VDSMVSTVAAVHTAAN 376

BLAST of Cp4.1LG16g02620 vs. ExPASy TrEMBL
Match: A0A7R6B0V1 (Cysteine proteinase 2 OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 708 bits (1827), Expect = 2.20e-256
Identity = 343/377 (90.98%), Postives = 361/377 (95.76%), Query Frame = 0

Query: 25  MVKGFSFLVVLSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHF 84
           MVK FS +V LSL+A +AIG+EV SGES+GDF+IRQVVDDGGVNGGSNGDDLLLGAEHHF
Sbjct: 1   MVKCFSLIVALSLLAVSAIGAEVFSGESDGDFVIRQVVDDGGVNGGSNGDDLLLGAEHHF 60

Query: 85  SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDSSATHGVTQFSDLTPSEFRRSF 144
           S+FKR+FGKSYAS EEHDHRFR+FKANLRRA+RHQALD SATHGVTQFSDLTPSEFRRSF
Sbjct: 61  SLFKRKFGKSYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120

Query: 145 LGLRDR-LRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG 204
           LGLR R L LPADANKAPILPTD LPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG
Sbjct: 121 LGLRGRRLGLPADANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG 180

Query: 205 ANFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDY 264
           ANFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLM+E DY
Sbjct: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMKENDY 240

Query: 265 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGV 324
           PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANL+KNGPLA+AINAV+MQTY+GGV
Sbjct: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGV 300

Query: 325 SCPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRGRNVC 384
           SCPYICSK LDHGVLLVGYGS  YAPIR+KEKPYWIIKNSWGA WGENG+YKICRGRN+C
Sbjct: 301 SCPYICSKHLDHGVLLVGYGSGAYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNIC 360

Query: 385 GVDSMVSTVAAVHTAAN 400
           GVDSMVSTVAAVHTAAN
Sbjct: 361 GVDSMVSTVAAVHTAAN 377

BLAST of Cp4.1LG16g02620 vs. ExPASy TrEMBL
Match: A0A0A0LPD0 (Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_2G249900 PE=3 SV=1)

HSP 1 Score: 694 bits (1792), Expect = 4.73e-251
Identity = 338/377 (89.66%), Postives = 361/377 (95.76%), Query Frame = 0

Query: 25  MVKGFSFLVVLSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHF 84
           MVK FS +VVLSL+AA+AIGSEV+SGES+GDFIIRQVVDDGGVN GSNGDDLLLGA+HHF
Sbjct: 1   MVKCFSLIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHF 60

Query: 85  SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDSSATHGVTQFSDLTPSEFRRSF 144
           S+FK++FGKSYAS EEHDHRFR+FKANL+RA+RHQALD SATHGVTQFSDLTPSEFRRSF
Sbjct: 61  SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120

Query: 145 LGLRDR-LRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG 204
           LGLR R L LPADANKAPILPTD LPTDFDWRD GAV+EVKNQGSCGSCWSFS TGALEG
Sbjct: 121 LGLRSRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEG 180

Query: 205 ANFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDY 264
           ANFLATGKLVSLSEQQLVDCDHECDPEEK SCDSGCNGGLMNSAFEYTLK+GGLM+E+DY
Sbjct: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY 240

Query: 265 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGV 324
           PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANL+KNGPLAVAINAV+MQTY+ GV
Sbjct: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGV 300

Query: 325 SCPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRGRNVC 384
           SCPYICSK LDHGVLLVGYGS GYAPIR+K+KPYWIIKNSWGA WGENG+YKICRGRN+C
Sbjct: 301 SCPYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNIC 360

Query: 385 GVDSMVSTVAAVHTAAN 400
           GVDSMVSTVAAVHTAAN
Sbjct: 361 GVDSMVSTVAAVHTAAN 377

BLAST of Cp4.1LG16g02620 vs. ExPASy TrEMBL
Match: A0A6J1CXX8 (cysteine protease RD19A-like OS=Momordica charantia OX=3673 GN=LOC111015466 PE=3 SV=1)

HSP 1 Score: 691 bits (1783), Expect = 1.03e-249
Identity = 335/375 (89.33%), Postives = 358/375 (95.47%), Query Frame = 0

Query: 25  MVKGFSFLVVLSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHF 84
           MV  FSF VVLS++A +AIG EVLS ESEGD +IRQVVDDGGVN GSNGDDLLLGAEHHF
Sbjct: 1   MVSYFSFFVVLSILAVSAIGGEVLSVESEGDNLIRQVVDDGGVNEGSNGDDLLLGAEHHF 60

Query: 85  SIFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDSSATHGVTQFSDLTPSEFRRSF 144
           S+FKR+FGKSYAS EEHDHRFR+F+ANLRRA+RHQALD SATHGVTQFSDLTPSEFR+SF
Sbjct: 61  SLFKRKFGKSYASQEEHDHRFRVFRANLRRAQRHQALDPSATHGVTQFSDLTPSEFRKSF 120

Query: 145 LGLRD-RLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG 204
           LGLR  RL LPADANKAPILP DDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG
Sbjct: 121 LGLRGPRLGLPADANKAPILPIDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG 180

Query: 205 ANFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDY 264
           ANFLATGKLVSLSEQQLVDCDHECDPEEK+SCD+GCNGGLMNSAFEYTLKAGGLM+EKDY
Sbjct: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKNSCDAGCNGGLMNSAFEYTLKAGGLMREKDY 240

Query: 265 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGV 324
           PYTGTDRGTCKF+KSKIAASVANFSVVSLDEEQIAANL+KNGPLA+AINAV+MQTY+GGV
Sbjct: 241 PYTGTDRGTCKFEKSKIAASVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGV 300

Query: 325 SCPYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRGRNVC 384
           SCPYICSK LDHGVLLVGYGSAGYAPIR+KEKPYWIIKNSWG  WGENG+YKIC+GRN+C
Sbjct: 301 SCPYICSKHLDHGVLLVGYGSAGYAPIRLKEKPYWIIKNSWGENWGENGYYKICKGRNIC 360

Query: 385 GVDSMVSTVAAVHTA 398
           GV+SMVSTVAAVHTA
Sbjct: 361 GVNSMVSTVAAVHTA 375

BLAST of Cp4.1LG16g02620 vs. TAIR 10
Match: AT4G39090.1 (Papain family cysteine protease )

HSP 1 Score: 574.7 bits (1480), Expect = 5.8e-164
Identity = 284/376 (75.53%), Postives = 318/376 (84.57%), Query Frame = 0

Query: 26  VKGFSFLVVLSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHFS 85
           +K +  + VLS    +   S+V  G+   D +IRQVV      GG+  +  +L +E HFS
Sbjct: 4   LKLYFSVFVLSFFIVSVSSSDVNDGD---DLVIRQVV------GGA--EPQVLTSEDHFS 63

Query: 86  IFKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDSSATHGVTQFSDLTPSEFRRSFL 145
           +FKR+FGK YASNEEHD+RF +FKANLRRARRHQ LD SATHGVTQFSDLT SEFR+  L
Sbjct: 64  LFKRKFGKVYASNEEHDYRFSVFKANLRRARRHQKLDPSATHGVTQFSDLTRSEFRKKHL 123

Query: 146 GLRDRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGAN 205
           G+R   +LP DANKAPILPT++LP DFDWRDHGAVT VKNQGSCGSCWSFS TGALEGAN
Sbjct: 124 GVRSGFKLPKDANKAPILPTENLPEDFDWRDHGAVTPVKNQGSCGSCWSFSATGALEGAN 183

Query: 206 FLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYPY 265
           FLATGKLVSLSEQQLVDCDHECDPEE DSCDSGCNGGLMNSAFEYTLK GGLM+E+DYPY
Sbjct: 184 FLATGKLVSLSEQQLVDCDHECDPEEADSCDSGCNGGLMNSAFEYTLKTGGLMKEEDYPY 243

Query: 266 TGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVSC 325
           TG D  TCK DKSKI ASV+NFSV+S+DEEQIAANL+KNGPLAVAINA YMQTY+GGVSC
Sbjct: 244 TGKDGKTCKLDKSKIVASVSNFSVISIDEEQIAANLVKNGPLAVAINAGYMQTYIGGVSC 303

Query: 326 PYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRGRNVCGV 385
           PYIC++RL+HGVLLVGYG+AGYAP R KEKPYWIIKNSWG  WGENGFYKIC+GRN+CGV
Sbjct: 304 PYICTRRLNHGVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGENGFYKICKGRNICGV 363

Query: 386 DSMVSTVAA-VHTAAN 401
           DSMVSTVAA V T A+
Sbjct: 364 DSMVSTVAATVSTTAH 368

BLAST of Cp4.1LG16g02620 vs. TAIR 10
Match: AT4G16190.1 (Papain family cysteine protease )

HSP 1 Score: 545.4 bits (1404), Expect = 3.7e-155
Identity = 266/374 (71.12%), Postives = 313/374 (83.69%), Query Frame = 0

Query: 29  FSFLVVLSLIAATAIGSEVLSGESEGDFI--IRQVVDDGGVNGGSNGDDLLLGAEHHFSI 88
           F FL+  +L+A  ++GS V+SGE    F+  IRQVV +         D+ LL AEHHF++
Sbjct: 6   FFFLIAATLLAG-SLGSTVISGEVTDGFVNPIRQVVPE-------ENDEQLLNAEHHFTL 65

Query: 89  FKRRFGKSYASNEEHDHRFRIFKANLRRARRHQALDSSATHGVTQFSDLTPSEFRRSFLG 148
           FK ++ K+YA+  EHDHRFR+FKANLRRARR+Q LD SA HGVTQFSDLTP EFRR FLG
Sbjct: 66  FKSKYEKTYATQVEHDHRFRVFKANLRRARRNQLLDPSAVHGVTQFSDLTPKEFRRKFLG 125

Query: 149 LRDR-LRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGAN 208
           L+ R  RLP D   APILPT DLPT+FDWR+ GAVT VKNQG CGSCWSFS  GALEGA+
Sbjct: 126 LKRRGFRLPTDTQTAPILPTSDLPTEFDWREQGAVTPVKNQGMCGSCWSFSAIGALEGAH 185

Query: 209 FLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYPY 268
           FLAT +LVSLSEQQLVDCDHECDP + +SCDSGC+GGLMN+AFEY LKAGGLM+E+DYPY
Sbjct: 186 FLATKELVSLSEQQLVDCDHECDPAQANSCDSGCSGGLMNNAFEYALKAGGLMKEEDYPY 245

Query: 269 TGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVSC 328
           TG D   CKFDKSKI ASV+NFSVVS DE+QIAANL+++GPLA+AINA++MQTY+GGVSC
Sbjct: 246 TGRDHTACKFDKSKIVASVSNFSVVSSDEDQIAANLVQHGPLAIAINAMWMQTYIGGVSC 305

Query: 329 PYICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRG-RNVCG 388
           PY+CSK  DHGVLLVG+GS+GYAPIR+KEKPYWIIKNSWGA WGE+G+YKICRG  N+CG
Sbjct: 306 PYVCSKSQDHGVLLVGFGSSGYAPIRLKEKPYWIIKNSWGAMWGEHGYYKICRGPHNMCG 365

Query: 389 VDSMVSTVAAVHTA 399
           +D+MVSTVAAVHT+
Sbjct: 366 MDTMVSTVAAVHTS 371

BLAST of Cp4.1LG16g02620 vs. TAIR 10
Match: AT2G21430.1 (Papain family cysteine protease )

HSP 1 Score: 545.0 bits (1403), Expect = 4.9e-155
Identity = 262/366 (71.58%), Postives = 304/366 (83.06%), Query Frame = 0

Query: 29  FSFLVVLSLIAATAIGSEVLSGESEGDFIIRQVVDDGGVNGGSNGDDLLLGAEHHFSIFK 88
           FS  ++   ++ +  G E        D +IRQVVD+         +  +L +E HF++FK
Sbjct: 9   FSVSLIFVFVSVSVCGDE--------DVLIRQVVDE--------TEPKVLSSEDHFTLFK 68

Query: 89  RRFGKSYASNEEHDHRFRIFKANLRRARRHQALDSSATHGVTQFSDLTPSEFRRSFLGLR 148
           ++FGK Y S EEH +RF +FKANL RA RHQ +D SA HGVTQFSDLT SEFRR  LG++
Sbjct: 69  KKFGKVYGSIEEHYYRFSVFKANLLRAMRHQKMDPSARHGVTQFSDLTRSEFRRKHLGVK 128

Query: 149 DRLRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGANFLA 208
              +LP DAN+APILPT +LP +FDWRD GAVT VKNQGSCGSCWSFSTTGALEGA+FLA
Sbjct: 129 GGFKLPKDANQAPILPTQNLPEEFDWRDRGAVTPVKNQGSCGSCWSFSTTGALEGAHFLA 188

Query: 209 TGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYPYTGT 268
           TGKLVSLSEQQLVDCDHECDPEE+ SCDSGCNGGLMNSAFEYTLK GGLM+EKDYPYTGT
Sbjct: 189 TGKLVSLSEQQLVDCDHECDPEEEGSCDSGCNGGLMNSAFEYTLKTGGLMREKDYPYTGT 248

Query: 269 DRGTCKFDKSKIAASVANFSVVSLDEEQIAANLLKNGPLAVAINAVYMQTYVGGVSCPYI 328
           D G+CK D+SKI ASV+NFSVVS++E+QIAANL+KNGPLAVAINA YMQTY+GGVSCPYI
Sbjct: 249 DGGSCKLDRSKIVASVSNFSVVSINEDQIAANLIKNGPLAVAINAAYMQTYIGGVSCPYI 308

Query: 329 CSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICRGRNVCGVDSM 388
           CS+RL+HGVLLVGYGSAG++  R+KEKPYWIIKNSWG  WGENGFYKIC+GRN+CGVDS+
Sbjct: 309 CSRRLNHGVLLVGYGSAGFSQARLKEKPYWIIKNSWGESWGENGFYKICKGRNICGVDSL 358

Query: 389 VSTVAA 395
           VSTVAA
Sbjct: 369 VSTVAA 358

BLAST of Cp4.1LG16g02620 vs. TAIR 10
Match: AT3G54940.2 (Papain family cysteine protease )

HSP 1 Score: 420.6 bits (1080), Expect = 1.4e-117
Identity = 202/342 (59.06%), Postives = 256/342 (74.85%), Query Frame = 0

Query: 55  DFIIRQVVDDGGVNGGSNGDDLLLGAEHHFSIFKRRFGKSYASNEEHDHRFRIFKANLRR 114
           D  IRQV  D   N     + L    E  F +F   +GK+Y++ EE+ HR  IF  N+ +
Sbjct: 25  DLTIRQVTAD---NRRIRPNLLGTHTESKFRLFMSDYGKNYSTREEYIHRLGIFAKNVLK 84

Query: 115 ARRHQALDSSATHGVTQFSDLTPSEFRRSFLGLRD--RLRLPADANKAPILPTDDLPTDF 174
           A  HQ +D SA HGVTQFSDLT  EF+R + G+ D    R      +AP++  D LP DF
Sbjct: 85  AAEHQMMDPSAVHGVTQFSDLTEEEFKRMYTGVADVGGSRGGTVGAEAPMVEVDGLPEDF 144

Query: 175 DWRDHGAVTEVKNQGSCGSCWSFSTTGALEGANFLATGKLVSLSEQQLVDCDHECDPEEK 234
           DWR+ G VTEVKNQG+CGSCW+FSTTGA EGA+F++TGKL+SLSEQQLVDCD  CDP++K
Sbjct: 145 DWREKGGVTEVKNQGACGSCWAFSTTGAAEGAHFVSTGKLLSLSEQQLVDCDQACDPKDK 204

Query: 235 DSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYPYTGTDRGTCKFDKSKIAASVANFSVVSL 294
            +CD+GC GGLM +A+EY ++AGGL +E+ YPYTG  RG CKFD  K+A  V NF+ + L
Sbjct: 205 KACDNGCGGGLMTNAYEYLMEAGGLEEERSYPYTG-KRGHCKFDPEKVAVRVLNFTTIPL 264

Query: 295 DEEQIAANLLKNGPLAVAINAVYMQTYVGGVSCPYICSKR-LDHGVLLVGYGSAGYAPIR 354
           DE QIAANL+++GPLAV +NAV+MQTY+GGVSCP ICSKR ++HGVLLVGYGS G++ +R
Sbjct: 265 DENQIAANLVRHGPLAVGLNAVFMQTYIGGVSCPLICSKRNVNHGVLLVGYGSKGFSILR 324

Query: 355 MKEKPYWIIKNSWGAKWGENGFYKICRGRNVCGVDSMVSTVA 394
           +  KPYWIIKNSWG KWGENG+YK+CRG ++CG++SMVS VA
Sbjct: 325 LSNKPYWIIKNSWGKKWGENGYYKLCRGHDICGINSMVSAVA 362

BLAST of Cp4.1LG16g02620 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 223.8 bits (569), Expect = 2.5e-58
Identity = 128/307 (41.69%), Postives = 173/307 (56.35%), Query Frame = 0

Query: 93  KSYASNEEHDHRFRIFKANLRRARRHQALDSSATH-GVTQFSDLTPSEFRRSFLGLR-DR 152
           K+Y    E + RF IFK NL+    H ++ +     G+T+F+DLT  EFR  +L  + +R
Sbjct: 52  KNYNGLGEKERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFADLTNDEFRAIYLRSKMER 111

Query: 153 LRLPADANKAPILPTDDLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGANFLATG 212
            R+P    K      D LP   DWR  GAV  VK+QGSCGSCW+FS  GA+EG N + TG
Sbjct: 112 TRVPVKGEKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCGSCWAFSAIGAVEGINQIKTG 171

Query: 213 KLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMQEKDYPYTGTDR 272
           +L+SLSEQ+LVDCD         S + GC GGLM+ AF++ ++ GG+  E+DYPY  TD 
Sbjct: 172 ELISLSEQELVDCD--------TSYNDGCGGGLMDYAFKFIIENGGIDTEEDYPYIATDV 231

Query: 273 GTCKFDKSKI-AASVANFSVVSLDEEQIAANLLKNGPLAVAINA--VYMQTYVGGVSCPY 332
             C  DK      ++  +  V  ++E+     L N P++VAI A     Q Y  GV    
Sbjct: 232 NVCNSDKKNTRVVTIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRAFQLYTSGVFTG- 291

Query: 333 ICSKRLDHGVLLVGYGSAGYAPIRMKEKPYWIIKNSWGAKWGENGFYKICR----GRNVC 391
            C   LDHGV+ VGYGS G        + YWI++NSWG+ WGE+G++K+ R        C
Sbjct: 292 TCGTSLDHGVVAVGYGSEG-------GQDYWIVRNSWGSNWGESGYFKLERNIKESSGKC 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P432968.1e-16375.53Cysteine protease RD19A OS=Arabidopsis thaliana OX=3702 GN=RD19A PE=1 SV=1[more]
Q9SUL15.3e-15471.12Probable cysteine protease RD19C OS=Arabidopsis thaliana OX=3702 GN=RD19C PE=2 S... [more]
P432956.9e-15471.58Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 S... [more]
P258049.3e-15171.47Cysteine proteinase 15A OS=Pisum sativum OX=3888 PE=2 SV=1[more]
Q107165.5e-14368.00Cysteine proteinase 1 OS=Zea mays OX=4577 GN=CCP1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023511874.11.42e-277100.00cysteine protease RD19A [Cucurbita pepo subsp. pepo][more]
KAG6571085.13.90e-27598.67Cysteine protease RD19A, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022944124.16.46e-27498.40cysteine protease RD19A-like [Cucurbita moschata] >KAG7010902.1 Cysteine proteas... [more]
XP_022986555.13.07e-27298.14cysteine protease RD19A-like [Cucurbita maxima][more]
ARA15252.14.54e-25690.98cysteine proteinase 2 [Citrullus lanatus][more]
Match NameE-valueIdentityDescription
A0A6J1FW383.13e-27498.40cysteine protease RD19A-like OS=Cucurbita moschata OX=3662 GN=LOC111448673 PE=3 ... [more]
A0A6J1JED61.49e-27298.14cysteine protease RD19A-like OS=Cucurbita maxima OX=3661 GN=LOC111484259 PE=3 SV... [more]
A0A7R6B0V12.20e-25690.98Cysteine proteinase 2 OS=Citrullus lanatus OX=3654 PE=2 SV=1[more]
A0A0A0LPD04.73e-25189.66Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_2G249900 PE=3 SV=1[more]
A0A6J1CXX81.03e-24989.33cysteine protease RD19A-like OS=Momordica charantia OX=3673 GN=LOC111015466 PE=3... [more]
Match NameE-valueIdentityDescription
AT4G39090.15.8e-16475.53Papain family cysteine protease [more]
AT4G16190.13.7e-15571.12Papain family cysteine protease [more]
AT2G21430.14.9e-15571.58Papain family cysteine protease [more]
AT3G54940.21.4e-11759.06Papain family cysteine protease [more]
AT3G19390.12.5e-5841.69Granulin repeat cysteine protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 335..345
score: 58.8
coord: 186..201
score: 64.12
coord: 357..363
score: 75.14
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 168..393
e-value: 1.0E-111
score: 387.1
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 168..391
e-value: 4.4E-75
score: 252.5
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 84..140
e-value: 1.6E-22
score: 90.8
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 84..140
e-value: 2.7E-12
score: 46.9
NoneNo IPR availableGENE3D3.90.70.10Cysteine proteinasescoord: 71..393
e-value: 5.6E-100
score: 337.2
NoneNo IPR availablePANTHERPTHR12411:SF799BNAA04G12170D PROTEINcoord: 61..393
NoneNo IPR availablePANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 61..393
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 186..197
IPR025660Cysteine peptidase, histidine active sitePROSITEPS00639THIOL_PROTEASE_HIScoord: 333..343
IPR025661Cysteine peptidase, asparagine active sitePROSITEPS00640THIOL_PROTEASE_ASNcoord: 357..376
IPR039417Papain-like cysteine endopeptidaseCDDcd02248Peptidase_C1Acoord: 169..392
e-value: 1.61372E-105
score: 308.399
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 82..392

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG16g02620.1Cp4.1LG16g02620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0005615 extracellular space
cellular_component GO:0005764 lysosome
molecular_function GO:0004197 cysteine-type endopeptidase activity
molecular_function GO:0008234 cysteine-type peptidase activity