Cp4.1LG16g02580 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG16g02580
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Bifunctional dihydrofolate reductase-thymidylate synthase
Location	Cp4.1LG16: 4546545 .. 4552237 (-)
RNA-Seq Expression	Cp4.1LG16g02580
Synteny	Cp4.1LG16g02580

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAGAATAAAAAGAAAGTAGAGCCACACTTCGATCTCGAATATATCCATTTCGGATCATAGACGGCGGCAGCCCGCAGGAAACGCGTCTTTGGATTATCCTCCGTTTACTTCTTCACTTTTCCACATAAACCCTCTTCCATTGGAGCCCAAAAAGTTCAAAAAACCCAAAAACATCGAAACGGGATAAAGAAAATAACCGGTTTTTCATCCTAAAAACTCCCCACCTGCTGGATTTCCCTTTCCCCTCCCTTTTCACCATTACTCTACCCGTTTCAGAAATACCCATTTCGTAAAACCCCCCAAAATCCAAAGAACACAGCGCCCTAAACCTAACCCTCTTCCAATCGAAAGTCAGCCTAAGTTTATCTTAGGCTTTTTCGAGTTGAATATTCTTAGCCCGCGTTTAATCTCGGTGGGTTGCGCCTAATTCTCCCTTTTTCCCCTGATTTTGCTATGTCATTTTGGGATTGGATTGTTAGGGACTGAGTTTTGGATTCTGTTATTGATTTTGCTTTCTGGGTTGGAGCTTAAATGCTCTTTCTGTAATTGGGTGATCGTCCAATGTTCTTATTATTCTTCATGCTTTTTGTTGATTGAATTTGCATTTGACCTGTGAGTAGTTTATTGCATGTGCCCTTTTGTTCTTTTTCTTTTTCATGCGTTGTTGGTTTGATTCTGTCTCTTGTTCGTCTCTTTATGACATTATCCCTTATCTCTATTGCCAAGCGAATGTGAGCGGTAAGGATTGTGTTTTTTAAATGATCTTGCCTGCAATTTGTTAGGTTGGGAAGAATCTGGAGGTAAAGTGAAGGAAAAAGATAAACAATTGTTTACATCTTTATCCTTGTTCTTTTGTTCTTTGATTTTGATGTTTTTAATTGATTCTGTTCATGTAAAACTATGGATTTGGAAGTCGGTTTCGTTTTTAAGTGGATTTCATTTGTTTGGAAGAATAATTAAGTGAATATGGAATTATGTACTGTTCCTTTGAACCAAAAGATAGATCAATTAGAAAAGTAAGCCATAGACGGAATGAAAGTTTAGCTTAATCCTCCTTTGAATGGTGTGATATCCCACATTGGTCGAGGAGGAGAACGAAACACCCTTTATAAGGGTGTGGTAACCTTTTTCCTAGCAAATGCGTTTTAAAGCCTTGAGGGGAAGCCCGAAAGGGAAAGCCCAAAGAGGACAATATCTACTAGTGGTGGGCCTGAGCCGTTACAAATAGTATCAGAGCCAGACACGGGGCGATGTGCTAGGGAGGAGGTTGTTCCCTGAAGGGGTAGACACGAGGCGGTGTGCCAATAAGGATGCTGGGCCCCGAAGGGGGTGGATTTAGGGGGGTCCCACATCAATTTGAAAAAGGAACGAGTGCCAGCGAGGACGCTGGGCCCTGAAGGGGGTGGATTGTGATATCCCACGTTGGTTGGGGAGGAGAACGAAACACCCTTTATAAGGGTGTGGAAACCTTTCCCTAGCAATCGTGTTTTAAAGCCTTGAGGGGGAGCTCGAAAGGGAAAACCCAAAGAGAACAATATCTGCTAGCGGTGGGCCTGGGCCGTTACAAACGGTGAACTATTTCTTTTTTTTATTGTGAAGCAACAATTTCATTGGTTTTACAAATCTTGTTCTGTGCCCCTTGGCATCTGTCTATGGAGAGTTATAATCTTCATCTATATTAAGCAGAAGTTTTTCAAGTTCATGATGAGTTTAAGATTGTTTTGTGAAATGGAAGTGTTTGTTCATTCAATAAAAGAGTTTTGAATGCTTTAATCCTTTGGATCATGTTCTATATGATTTATTTCTGAAGTTTTCTGGTTGTCTTAAATTTCAGGTTCTACCGCACTTCCGTGGAAGCTGGTCGTGCGGGGAAAAATCATTGAAGTCACCGTTATTTAGGTTCGGTCAAGTTTTTAGTTCTAATATGGCTGCTGATCCTCTGACAAGCTCGAATGGAAATGCCAATGTAAATGCACCACCTAATCCGCGAAGGACTTATCAAGTCGTTGTAGCTGCTACTGAAAATATGGGTATTGGTAAGGATGGGAAACTTCCATGGAGGTTGCCTTCTGATCTCAAATTTTTTAAGAACATAACCACGACAACATCAGATCATGGGAAAAGGAATGCAGTTCTAATGGGTCGTAAAACATGGGAGAGTATTCCGGCTGAGTATCGACCTCTACCTGGTCGTCTCAATGTTGTTCTGACTCGTTCTGGAAGTTTCGACATTGCCACTGTCGAAAATGTTATAATATGTGGTAGTATGACGTCTGCTTTGGAATTATTAGCAGCTTCGCCTTACTGTTTGTCAATAGAGAAAGTGTTTGTTATAGGTGGTGGTGAGATATTAAGGTGTGCACTTGTTTTCGAGACCTTCGTTAGTGTACTATTTTTTTGTTTCGGTGCACTGATTCTGTTTTTAATGCAGGGAAGCTCTGAATGCCCCCGAGTGTGATGCGGTCCACGTTACTGAAATCGAGACAAACGTTGAATGCGACACCTTCATACCAGCTATTGACAAGTCTATGTTTCAGCCATGGTACTCATCCTTTCCCGTGGTGGAAAATAACTTACGATATTCGTTCACTAGTTATGTTCGTGTGAGGAGCTCAACCGAACCACCACCCATTGATCAGAACAATGGTCTGGTCTCTCATAACAAACCCGATTCTTTTAACTTCGAAGCTAAGAACTTCTCTTTCCTACCCAAGGAGATTTTTGAGAGGCACGAGGAGTACTTGTACCTAAAACTTGTTCAAGAAATCATCTCAGACGGTACTTCCAAGGATGATAGAACGGGAACGGGTACTTTATCAAAATTTGGTTGCCAGGTAACGTTATCTTTCTTATTATTTGACATATTTATCTTTTATACAAGTTGCTCTATTTCGAATGCTACATGTTCATCTCTTGTTCGTGGTATCTGTACAGATGCGGTACAACTTGCGCAAAACTTTTCCGCTGCTTACAACTAAGGCATGTGCTCGTTTCGTATTTTGGTTTCCTTTGTAGCTGTAGCATGAAAGAAAAACTCCCTTTTTTACTCGTCTTGTTTCCGTTTTTCAATTTCTTAAATCGGGTGCATCGTATATCGTAATGGTTTAGTCGTTGACATATATTCGTACCACGTGATCCTTCAGAAAGTATTTTGGCGAGGGGTTGTTGAAGAACTTCTTTGGTTCATCAGTGGTTCAACCAATGCAAAGGTAAGGCGCTATGCTATCACTAGCACTTAAAGCTTTACTTCACTAGTTTGTGGTTGTGATTGTACAACCCCGCACCCCGCACCCCGCACGTTGAGTTAGAATTTCTTGGTTTAGGAATTACGAACTAAGCGATTAAGTGTTTTGCTTTCTTGGTTTAGTGGTTGCGGTGTTTCGGTTCATAGCTTGGGGCCTTGTATGATTTGTGTTTCGGTTCATAGTCTCCCTAGTTTTCTTCTGTGAAATCCCACGTTGGTTGGGGAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACCTCTCGCTAGTAGATGCGTTTTAAAAACCTTGAGGGGAAGCCTAGAAGGGAAATCCCAAAGAGGACGATATCTGCTAGTGGTGGGCTTGGGCTTGGGCTGCTCTCAACCATTGTTTGTAGGGCTAGACCTACTCCATTGTGGTTTATCTTGCATTGGCATCTTGTTTTGGCCTACAAGTGTGGCCTTATTTTCGACATTATCAACAGGTTCTTCAGGAGAAAGGCATTCATATATGGGATGGCAACGCGTCGAGAGATTACCTCGATAGGCAATTACTTATACCTTCGAAATTTGATTCTTTTTAATATGAAACTAGAGAAGTTTGAGTTTTTTTGTATATAATTTGAGCTATAATTCTGTTTTTTTTTTTGGGTAATCTTAGTATTGGTTTAAGAGAAAGGGAGGAAGGCGACTTGGGACCCGTATACGGGTTTCAGTGGCGACACTTCGGTGCTAGGTTCGTCGATAACTTCGATGATCTCTTAAGTTTGTTTTTATTTTATTGCGGATTCGAGCTTTAATGCTTTCGACGCTTCTTCTACAGGTATACTGACATGCATGCTGACTACACTGGTCAAGGATTTGATCAGTTGCTAGATGTTATTAACAAGATAAAAAATAATCCCGATGATCGGAGGATCGTTCTTTCTGCGTGGAATCCATCTGATCTCAAACTGATGGCACTCCCGCCTTGCCACATGTTCGCTCAGGTTGGACTCGAGTGCATGATGTTCCGTGTCTTTAACTTGTTTCCTTAGCGTTGTTTATTGATCGCCTTTTGCAGTTTTATGTAGCGAACGGGGAGTTATCGTGTCAAATGTACCAGCGCTCTGCTGATATGGGCCTTGGTGTTCCATTTAATATTGCGTCGTATGCTCTTCTGACGTGCATGATTGCTCATGTTTGTGGTACGCTACTCTATATTGATCAGTAGTTTGTTTTCTACTATTGTGAGATCCCACATCGATTGGAGAGGGGAACGGATCATTCTTTATAAGGGTGTAGAAACTTCTCCGTAATAAACGCGTTTTAAAACTGTGAGGCTAACGACGTATCTGTTAGTAGTAGGCTTGGGTTGTTACAAATGGTATCAGAGCCAGACACCGGGCGGTGTGCCAGCAGGTCACTGGGCTCCCAAGGGGGTGGATTGTGAGATCCCACATCGGTTGGAGAGGGGAACAAAACATTGTTTATAAGGATGTGGAAACCTCTCCCTAACAGACGCGTTTTAAAACCGTGAGGCTGACGACCATATGTAACTGGACAAACCGGAAAATGATTTAATTATCTTTATACACATCTATATGGCTCTTTAAACATTCCTCCCCTACATTTATACTTTTAGTTGCATTAAAAAGTTTATTCTTCCTACGTGCAGATCTTGCTTACGGAGACTTCATACATGTGATCGGAGACGCTCATGTTTACCGTACTCACGTGCGGCCATTGCAGGAACAGCTTCAGAAACTCCCGAAACCGTTCCCTGTAAGTCGGAAAAGTATAATTACATGCTTCTTCCCTGCTAGCGTTCACTAACTTGCTGTTTTTAATATTACACCGGCCTTACCGTATTGTAGGTTTTGAAAATAAACCCCGAGAAGAAGGATATCGATTCGTTAGTGGCAGCTGATTTCGAGCTAACAGGTTATGACCCTCATGAGAAGATAGCAATGAAAATGGCAGTATAGAACATGATCTAGCTGCCCCAGCAGACAAAGGAGAAGACTATGTTCCTTCTACCACATATTCTCCAAATTTTGAGGTAAGTACGCTATAATCACCATGAAAAATGTCGGTTTTTAGTTGTTTTGAAATTGATCGTACCAGGTCGAAGAGCTGGTGTATGAAAGCGGTCGTGTTATTCCATGGTACTAAAGCGTTTTGAGCGATATTTCAGGTACGAGCACCCGAAGCTTTGGTTGATATGATCTGCAAATAAAAAGTGTTAAGTGGACATACTTTATACTCTTGTCATATGTAGTGAGAATCCTTTAGTCCCTATGGTTGGTTCTAAAGTGATTTAGTTCGGTTTTGTATTTGCTATAGATTTGTTCTCGATGAAAAAATGGAACGGTTTCACACACGAATACACGGTCGGTACTATTATAATACCATGCTACGTACAAATATAATACAATCTCGATCGATCTTTCTTGGAGTTTATCTGGTTTTGAAACGATGGTGAAT

mRNA sequence

AGAGAATAAAAAGAAAGTAGAGCCACACTTCGATCTCGAATATATCCATTTCGGATCATAGACGGCGGCAGCCCGCAGGAAACGCGTCTTTGGATTATCCTCCGTTTACTTCTTCACTTTTCCACATAAACCCTCTTCCATTGGAGCCCAAAAAGTTCAAAAAACCCAAAAACATCGAAACGGGATAAAGAAAATAACCGGTTTTTCATCCTAAAAACTCCCCACCTGCTGGATTTCCCTTTCCCCTCCCTTTTCACCATTACTCTACCCGTTTCAGAAATACCCATTTCGTAAAACCCCCCAAAATCCAAAGAACACAGCGCCCTAAACCTAACCCTCTTCCAATCGAAAGTCAGCCTAAGTTTATCTTAGGCTTTTTCGAGTTGAATATTCTTAGCCCGCGTTTAATCTCGGTTCTACCGCACTTCCGTGGAAGCTGGTCGTGCGGGGAAAAATCATTGAAGTCACCGTTATTTAGGTTCGGTCAAGTTTTTAGTTCTAATATGGCTGCTGATCCTCTGACAAGCTCGAATGGAAATGCCAATGTAAATGCACCACCTAATCCGCGAAGGACTTATCAAGTCGTTGTAGCTGCTACTGAAAATATGGGTATTGGTAAGGATGGGAAACTTCCATGGAGGTTGCCTTCTGATCTCAAATTTTTTAAGAACATAACCACGACAACATCAGATCATGGGAAAAGGAATGCAGTTCTAATGGGTCGTAAAACATGGGAGAGTATTCCGGCTGAGTATCGACCTCTACCTGGTCGTCTCAATGTTGTTCTGACTCGTTCTGGAAGTTTCGACATTGCCACTGTCGAAAATGTTATAATATGTGGTAGTATGACGTCTGCTTTGGAATTATTAGCAGCTTCGCCTTACTGTTTGTCAATAGAGAAAGTGTTTGTTATAGGTGGTGGTGAGATATTAAGGGAAGCTCTGAATGCCCCCGAGTGTGATGCGGTCCACGTTACTGAAATCGAGACAAACGTTGAATGCGACACCTTCATACCAGCTATTGACAAGTCTATGTTTCAGCCATGGTACTCATCCTTTCCCGTGGTGGAAAATAACTTACGATATTCGTTCACTAGTTATGTTCGTGTGAGGAGCTCAACCGAACCACCACCCATTGATCAGAACAATGGTCTGGTCTCTCATAACAAACCCGATTCTTTTAACTTCGAAGCTAAGAACTTCTCTTTCCTACCCAAGGAGATTTTTGAGAGGCACGAGGAGTACTTGTACCTAAAACTTGTTCAAGAAATCATCTCAGACGGTACTTCCAAGGATGATAGAACGGGAACGGGTACTTTATCAAAATTTGGTTGCCAGATGCGGTACAACTTGCGCAAAACTTTTCCGCTGCTTACAACTAAGGCATGTGCTCGTTTCGTATTTTGTATTGGTTTAAGAGAAAGGGAGGAAGGCGACTTGGGACCCGTATACGGGTTTCAGTGGCGACACTTCGGTGCTAGGTATACTGACATGCATGCTGACTACACTGGTCAAGGATTTGATCAGTTGCTAGATGTTATTAACAAGATAAAAAATAATCCCGATGATCGGAGGATCGTTCTTTCTGCGTGGAATCCATCTGATCTCAAACTGATGGCACTCCCGCCTTGCCACATGTTCGCTCAGTTTTATGTAGCGAACGGGGAGTTATCGTGTCAAATGTACCAGCGCTCTGCTGATATGGGCCTTGATCTTGCTTACGGAGACTTCATACATGTGATCGGAGACGCTCATGTTTACCGTACTCACGTGCGGCCATTGCAGGAACAGCTTCAGAAACTCCCGAAACCGTTCCCTGTTTTGAAAATAAACCCCGAGAAGAAGGATATCGATTCGTTAGTGGCAGCTGATTTCGAGCTAACAGGTTATGACCCTCATGAGAAGATAGCAATGAAAATGGCAGTATAGAACATGATCTAGCTGCCCCAGCAGACAAAGGAGAAGACTATGTTCCTTCTACCACATATTCTCCAAATTTTGAGGTACGAGCACCCGAAGCTTTGGTTGATATGATCTGCAAATAAAAAGTGTTAAGTGGACATACTTTATACTCTTGTCATATGTAGTGAGAATCCTTTAGTCCCTATGGTTGGTTCTAAAGTGATTTAGTTCGGTTTTGTATTTGCTATAGATTTGTTCTCGATGAAAAAATGGAACGGTTTCACACACGAATACACGGTCGGTACTATTATAATACCATGCTACGTACAAATATAATACAATCTCGATCGATCTTTCTTGGAGTTTATCTGGTTTTGAAACGATGGTGAAT

Coding sequence (CDS)

ATGGCTGCTGATCCTCTGACAAGCTCGAATGGAAATGCCAATGTAAATGCACCACCTAATCCGCGAAGGACTTATCAAGTCGTTGTAGCTGCTACTGAAAATATGGGTATTGGTAAGGATGGGAAACTTCCATGGAGGTTGCCTTCTGATCTCAAATTTTTTAAGAACATAACCACGACAACATCAGATCATGGGAAAAGGAATGCAGTTCTAATGGGTCGTAAAACATGGGAGAGTATTCCGGCTGAGTATCGACCTCTACCTGGTCGTCTCAATGTTGTTCTGACTCGTTCTGGAAGTTTCGACATTGCCACTGTCGAAAATGTTATAATATGTGGTAGTATGACGTCTGCTTTGGAATTATTAGCAGCTTCGCCTTACTGTTTGTCAATAGAGAAAGTGTTTGTTATAGGTGGTGGTGAGATATTAAGGGAAGCTCTGAATGCCCCCGAGTGTGATGCGGTCCACGTTACTGAAATCGAGACAAACGTTGAATGCGACACCTTCATACCAGCTATTGACAAGTCTATGTTTCAGCCATGGTACTCATCCTTTCCCGTGGTGGAAAATAACTTACGATATTCGTTCACTAGTTATGTTCGTGTGAGGAGCTCAACCGAACCACCACCCATTGATCAGAACAATGGTCTGGTCTCTCATAACAAACCCGATTCTTTTAACTTCGAAGCTAAGAACTTCTCTTTCCTACCCAAGGAGATTTTTGAGAGGCACGAGGAGTACTTGTACCTAAAACTTGTTCAAGAAATCATCTCAGACGGTACTTCCAAGGATGATAGAACGGGAACGGGTACTTTATCAAAATTTGGTTGCCAGATGCGGTACAACTTGCGCAAAACTTTTCCGCTGCTTACAACTAAGGCATGTGCTCGTTTCGTATTTTGTATTGGTTTAAGAGAAAGGGAGGAAGGCGACTTGGGACCCGTATACGGGTTTCAGTGGCGACACTTCGGTGCTAGGTATACTGACATGCATGCTGACTACACTGGTCAAGGATTTGATCAGTTGCTAGATGTTATTAACAAGATAAAAAATAATCCCGATGATCGGAGGATCGTTCTTTCTGCGTGGAATCCATCTGATCTCAAACTGATGGCACTCCCGCCTTGCCACATGTTCGCTCAGTTTTATGTAGCGAACGGGGAGTTATCGTGTCAAATGTACCAGCGCTCTGCTGATATGGGCCTTGATCTTGCTTACGGAGACTTCATACATGTGATCGGAGACGCTCATGTTTACCGTACTCACGTGCGGCCATTGCAGGAACAGCTTCAGAAACTCCCGAAACCGTTCCCTGTTTTGAAAATAAACCCCGAGAAGAAGGATATCGATTCGTTAGTGGCAGCTGATTTCGAGCTAACAGGTTATGACCCTCATGAGAAGATAGCAATGAAAATGGCAGTATAG

Protein sequence

MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTTTSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALELLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQPWYSSFPVVENNLRYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIFERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTKACARFVFCIGLREREEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLDLAYGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPVLKINPEKKDIDSLVAADFELTGYDPHEKIAMKMAV

Homology

[Close]

BLAST of Cp4.1LG16g02580 vs. ExPASy Swiss-Prot
Match: P51820 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 4.9e-207
Identity = 362/531 (68.17%), Postives = 410/531 (77.21%), Query Frame = 0


Query: 1   MAADPLTSSNGNAN--VNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNIT 60
           M +D    SNG++N  VN  PN +RTYQVVVAAT++ GIGKDGKLPWRLP+DLKFFK IT
Sbjct: 1   MPSDSSVISNGHSNGSVNPLPNLQRTYQVVVAATQDWGIGKDGKLPWRLPTDLKFFKEIT 60

Query: 61  TTTSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSA 120
             TS+ GK+NA++MGRKTWESIP EYRPL GRLNVVLTRSGSFDIAT ENV+ICGSM+SA
Sbjct: 61  MKTSEPGKKNAIVMGRKTWESIPLEYRPLSGRLNVVLTRSGSFDIATAENVVICGSMSSA 120

Query: 121 LELLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMF 180
           LELLAASPY LSIEKVFVIGGG+I REALN P C+A+H+TEI++++ECDTF+P +D ++F
Sbjct: 121 LELLAASPYSLSIEKVFVIGGGQIFREALNVPGCEAIHLTEIQSSIECDTFMPPVDFTIF 180

Query: 181 QPWYSSFPVVENNLRYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFNFEAKNFSFLPK 240
           +PWYSSFP VENN+RYSFT+YVRVRSS       QN   +  N  +S  FE K+FSFLPK
Sbjct: 181 RPWYSSFPKVENNIRYSFTTYVRVRSSA-AESAGQNIDPLLDNNSESMKFEVKDFSFLPK 240

Query: 241 EIFERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK----- 300
            I ERHEEYLYLKLVQ+II++GT+K DRTGTGTLSKFGCQMR+NLR  FPLLTTK     
Sbjct: 241 MISERHEEYLYLKLVQDIIAEGTTKGDRTGTGTLSKFGCQMRFNLRGNFPLLTTKKVFWR 300

Query: 301 ------------------------------ACARFVFCIGLREREEGDLGPVYGFQWRHF 360
                                         A   ++  +GL EREEGDLGPVYGFQWRHF
Sbjct: 301 GVVEELLWFISGSTNAKVLQEKGIHIWDGNASREYLDGVGLTEREEGDLGPVYGFQWRHF 360

Query: 361 GARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFY 420
           GARYTDMH DY+GQGFDQLLDVINKIK NPDDRRI+LSAWNP DLKLMALPPCHMFAQFY
Sbjct: 361 GARYTDMHHDYSGQGFDQLLDVINKIKRNPDDRRIILSAWNPVDLKLMALPPCHMFAQFY 420

Query: 421 VANGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHV 475
           VA+GELSCQMYQRSADMGL                    DL  GDFIHVIGDAH+YR HV
Sbjct: 421 VAHGELSCQMYQRSADMGLGIPFNIASYALLTCMIAHVCDLIPGDFIHVIGDAHIYRNHV 480

[Close]

BLAST of Cp4.1LG16g02580 vs. ExPASy Swiss-Prot
Match: P45350 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 4.7e-202
Identity = 353/530 (66.60%), Postives = 406/530 (76.60%), Query Frame = 0


Query: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT 60
           MA++ L +    + +   P+P+RTYQVVVAAT+NMGIGKDGKLPWRLPSD+KFFK++T T
Sbjct: 1   MASELLANPTNGSGITR-PDPQRTYQVVVAATQNMGIGKDGKLPWRLPSDMKFFKDVTMT 60

Query: 61  TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120
           TSD  KRNAV+MGRKTWESIP ++RPLPGRLNVVLTRSGSFDIATVENV+ICGSM SALE
Sbjct: 61  TSDPLKRNAVIMGRKTWESIPIQHRPLPGRLNVVLTRSGSFDIATVENVVICGSMISALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180
           LLA SPYC+S+EKVFVIGGG+I REALNAP CDAVH+TEIE ++ECDTFIP +D+S+FQP
Sbjct: 121 LLAGSPYCVSVEKVFVIGGGQIYREALNAPGCDAVHITEIEEHIECDTFIPLLDESVFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEI 240
           WYSSFP+VEN +RY FT+YVRVR+S       Q NG  S +K DS NFE +NFSFLPK +
Sbjct: 181 WYSSFPLVENKIRYCFTTYVRVRNSV-AELTSQTNGCSSDSKSDSGNFEIQNFSFLPKTV 240

Query: 241 FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK------- 300
           FE+HEEYLYL LV+ IIS+G +K+DRT TGT+S FGCQMR+NLRK+FPLLTTK       
Sbjct: 241 FEKHEEYLYLGLVENIISNGVTKNDRTRTGTVSIFGCQMRFNLRKSFPLLTTKKVFWRGV 300

Query: 301 ----------------------------ACARFVFCIGLREREEGDLGPVYGFQWRHFGA 360
                                           ++  IGL +REEGDLGP+YGFQWRHFGA
Sbjct: 301 VEELLWFISGSTNAKILKEKGVNIWEGNGSREYLDSIGLTDREEGDLGPIYGFQWRHFGA 360

Query: 361 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 420
           RYTDMHADY+GQGFDQLLDVI+KIKNNPDDRRI+ SAWNPSDL+LMALPPCHMFAQFYVA
Sbjct: 361 RYTDMHADYSGQGFDQLLDVISKIKNNPDDRRIIQSAWNPSDLRLMALPPCHMFAQFYVA 420

Query: 421 NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 475
           NGELSCQMYQRSADMGL                    DL  GDF+H IGDAHVY  H+  
Sbjct: 421 NGELSCQMYQRSADMGLGVPFNIAAYALLTCMIAHVCDLVPGDFVHSIGDAHVYSNHLSD 480

[Close]

BLAST of Cp4.1LG16g02580 vs. ExPASy Swiss-Prot
Match: Q05762 (Bifunctional dihydrofolate reductase-thymidylate synthase 1 OS=Arabidopsis thaliana OX=3702 GN=THY-1 PE=1 SV=2)

HSP 1 Score: 702.6 bits (1812), Expect = 3.1e-201
Identity = 346/523 (66.16%), Postives = 401/523 (76.67%), Query Frame = 0


Query: 7   TSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTTTSDHGK 66
           T+ N +        P+RTYQVVVAAT+ MGIGKDGKLPW LP+DLKFFK+IT TTSD  K
Sbjct: 4   TTLNDSVTTTLASEPQRTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDITLTTSDSSK 63

Query: 67  RNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALELLAASP 126
           +NAV+MGRKTWESIP +YRPL GRLNVVLTRSG FDIA  ENV+ C S+ SAL+LLAA P
Sbjct: 64  KNAVVMGRKTWESIPIKYRPLSGRLNVVLTRSGGFDIANTENVVTCSSVDSALDLLAAPP 123

Query: 127 YCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQPWYSSFP 186
           YCLSIE+VFVIGGG+ILREALN P CDA+H+TEI+T+V+CDTFIPAID S++QPW SSFP
Sbjct: 124 YCLSIERVFVIGGGDILREALNRPSCDAIHLTEIDTSVDCDTFIPAIDTSVYQPWSSSFP 183

Query: 187 VVENNLRYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIFERHEE 246
           V EN LR+ FT++VRV+SS +    D++NG        S  F+ K F FLPK +F++HEE
Sbjct: 184 VTENGLRFCFTTFVRVKSSADESS-DESNG------SQSLQFDGKKFLFLPKMVFDQHEE 243

Query: 247 YLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK------------- 306
           +LYL +V++IIS+G  K+DRTGTGTLSKFGCQM++NLR++FPLLTTK             
Sbjct: 244 FLYLNMVEDIISNGNVKNDRTGTGTLSKFGCQMKFNLRRSFPLLTTKRVFWRGVVEELLW 303

Query: 307 ----------------------ACARFVFCIGLREREEGDLGPVYGFQWRHFGARYTDMH 366
                                 A   ++  IGL EREEGDLGPVYGFQWRHFGA+YTDMH
Sbjct: 304 FISGSTNAKVLQEKGIHIWDGNASREYLDGIGLTEREEGDLGPVYGFQWRHFGAKYTDMH 363

Query: 367 ADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVANGELSC 426
           ADYTGQGFDQL+DVI+KIKNNPDDRRI++SAWNPSDLKLMALPPCHMFAQFYVA GELSC
Sbjct: 364 ADYTGQGFDQLVDVIDKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFAQFYVAEGELSC 423

Query: 427 QMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRPLQEQLQ 475
           QMYQRSADMGL                    DL  GDFIHV+GDAHVY+THVRPLQEQL 
Sbjct: 424 QMYQRSADMGLGVPFNIASYSLLTCMLAHVCDLVPGDFIHVLGDAHVYKTHVRPLQEQLL 483

[Close]

BLAST of Cp4.1LG16g02580 vs. ExPASy Swiss-Prot
Match: O81395 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Zea mays OX=4577 GN=DRTS PE=2 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 4.6e-197
Identity = 336/519 (64.74%), Postives = 398/519 (76.69%), Query Frame = 0


Query: 13  ANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTTTSDHGKRNAVLM 72
           AN ++   P+R YQVVVA T +MGIGKDG LPW+LP DLKFFK +T TTSD  K+NAV+M
Sbjct: 6   ANGDSQGRPQRNYQVVVAGTRDMGIGKDGVLPWKLPGDLKFFKELTLTTSDPVKKNAVIM 65

Query: 73  GRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALELLAASPYCLSIE 132
           GRKTWESIP + RPLPGRLNV+LTRSGSFD ATVENV+ICGSM SALELLA++PYCLSIE
Sbjct: 66  GRKTWESIPVKSRPLPGRLNVILTRSGSFDFATVENVVICGSMESALELLASTPYCLSIE 125

Query: 133 KVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQPWYSSFPVVENNL 192
           KVFVIGGG++LRE L  P C+A+H+T+I++++ECDTFIP +D S+FQPWYSSFPV+E+N+
Sbjct: 126 KVFVIGGGQVLREYLKGPACEAIHLTDIQSSIECDTFIPPVDFSVFQPWYSSFPVIESNI 185

Query: 193 RYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFN--FEAKNFSFLPKEIFERHEEYLYL 252
           R+SF S+VRVR S       ++NG  S  + D+ N  FE +NFSFLPK +++RHEEY YL
Sbjct: 186 RHSFVSFVRVRKSV--AETHESNGKES-TEVDTKNDKFETENFSFLPKMVYDRHEEYQYL 245

Query: 253 KLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK----------------- 312
            LV++II  G  K+DRTGTGTLSKFGCQMR+NLRK FPLLTTK                 
Sbjct: 246 NLVEDIIRSGAQKNDRTGTGTLSKFGCQMRFNLRKNFPLLTTKRVFWRGVVEELLWFISG 305

Query: 313 ------------------ACARFVFCIGLREREEGDLGPVYGFQWRHFGARYTDMHADYT 372
                             A   ++  +GL  REEGDLGP+YGFQWRHFGA YTDMHADYT
Sbjct: 306 STNAKVLQEKGIHIWDGNASREYLNSVGLAHREEGDLGPIYGFQWRHFGAEYTDMHADYT 365

Query: 373 GQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQ 432
           G+GFDQL+DVI+KIKN+P+DRRI+LSAWNPSDLK MALPPCHMFAQFYV NGELSCQMYQ
Sbjct: 366 GKGFDQLMDVIDKIKNDPEDRRIILSAWNPSDLKKMALPPCHMFAQFYVENGELSCQMYQ 425

Query: 433 RSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRPLQEQLQKLPK 475
           RSADMGL                    DL+ GDF+HVIGDAHVYR HVR L+EQ+QK+PK
Sbjct: 426 RSADMGLGVPFNIASYSLLTYMIAQVCDLSPGDFVHVIGDAHVYRNHVRALEEQIQKMPK 485

[Close]

BLAST of Cp4.1LG16g02580 vs. ExPASy Swiss-Prot
Match: Q05763 (Bifunctional dihydrofolate reductase-thymidylate synthase 2 OS=Arabidopsis thaliana OX=3702 GN=THY-2 PE=2 SV=2)

HSP 1 Score: 687.6 bits (1773), Expect = 1.0e-196
Identity = 344/519 (66.28%), Postives = 392/519 (75.53%), Query Frame = 0


Query: 12  NANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTTTSDHGKRNAVL 71
           N NV     P+ TYQVVVAAT+ MGIGKDGKLPW LP+DLKFFK++T +TSD  K+NAV+
Sbjct: 53  NGNVIMTSKPQSTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDLTLSTSDSAKKNAVV 112

Query: 72  MGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALELLAASPYCLSI 131
           MGRKTWESIP +YRPL GRLNVVL+RS  FDIA  ENV+ C S+ SAL+LLAA P+ LSI
Sbjct: 113 MGRKTWESIPKKYRPLSGRLNVVLSRSSGFDIANTENVVTCSSIDSALDLLAAPPFSLSI 172

Query: 132 EKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQPWYSSFPVVENN 191
           EKVFVIGGG+ILREALN P C+A+H+TEI+T+++CDTFIP +D S +QPW SSFP+ EN 
Sbjct: 173 EKVFVIGGGDILREALNKPSCEAIHITEIDTSIDCDTFIPTVDTSAYQPWCSSFPICENG 232

Query: 192 LRYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFNFEAKNF-SFLPKEIFERHEEYLYL 251
           LR+SFT++VRV+SS+     D+++G            + K F S LPK IF+RHEEYLYL
Sbjct: 233 LRFSFTTHVRVKSSSAGEASDESDG------SKVLQVDWKKFSSVLPKMIFDRHEEYLYL 292

Query: 252 KLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK----------------- 311
            LV+EIIS+G  KDDRTGTGTLSKFGCQM++NLR+ FPLLTTK                 
Sbjct: 293 NLVKEIISNGNLKDDRTGTGTLSKFGCQMKFNLRRNFPLLTTKRVFWRGVVEELLWFISG 352

Query: 312 ------------------ACARFVFCIGLREREEGDLGPVYGFQWRHFGARYTDMHADYT 371
                             A   ++  IGL EREEGDLGPVYGFQWRHFGA+YTDMHADYT
Sbjct: 353 STNAKVLQEKGIRIWDGNASRAYLDGIGLTEREEGDLGPVYGFQWRHFGAKYTDMHADYT 412

Query: 372 GQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQ 431
           GQGFDQLLDVINKIKNNPDDRRI++SAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQ
Sbjct: 413 GQGFDQLLDVINKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQ 472

Query: 432 RSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRPLQEQLQKLPK 475
           RSADMGL                    DL  GDFIHVIGDAHVY+ HVRPLQEQL+  PK
Sbjct: 473 RSADMGLGVPFNIASYSLLTCILAHVCDLVPGDFIHVIGDAHVYKNHVRPLQEQLENPPK 532

[Close]

BLAST of Cp4.1LG16g02580 vs. NCBI nr
Match: XP_023513067.1 (bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita pepo subsp. pepo] >XP_023513068.1 bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 931 bits (2407), Expect = 0.0
Identity = 467/529 (88.28%), Postives = 469/529 (88.66%), Query Frame = 0


Query: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT 60
           MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT
Sbjct: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT 60

Query: 61  TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120
           TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE
Sbjct: 61  TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEI 240
           WYSSFPVVENNLRYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEI
Sbjct: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEI 240

Query: 241 FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK------- 300
           FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK       
Sbjct: 241 FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTKKVFWRGV 300

Query: 301 ----------------------------ACARFVFCIGLREREEGDLGPVYGFQWRHFGA 360
                                       A   ++  IGLREREEGDLGPVYGFQWRHFGA
Sbjct: 301 VEELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGA 360

Query: 361 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 420
           RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 361 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 420

Query: 421 NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 474
           NGELSCQMYQRSADMGL                    DLAYGDFIHVIGDAHVYRTHVRP
Sbjct: 421 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAYGDFIHVIGDAHVYRTHVRP 480

[Close]

BLAST of Cp4.1LG16g02580 vs. NCBI nr
Match: XP_022944443.1 (bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita moschata] >XP_022944444.1 bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita moschata])

HSP 1 Score: 919 bits (2375), Expect = 0.0
Identity = 462/529 (87.33%), Postives = 466/529 (88.09%), Query Frame = 0


Query: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT 60
           MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNIT T
Sbjct: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITAT 60

Query: 61  TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120
           TSDHGKRNA+LMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE
Sbjct: 61  TSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEI 240
           WYSSFPVVENNLRYSFTSYVRVRSSTEPP IDQNNGLVSHNKPDSFNFEAKNFSFLPKEI
Sbjct: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPP-IDQNNGLVSHNKPDSFNFEAKNFSFLPKEI 240

Query: 241 FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK------- 300
           FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLR+TFPLLTTK       
Sbjct: 241 FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTKKVFWRGV 300

Query: 301 ----------------------------ACARFVFCIGLREREEGDLGPVYGFQWRHFGA 360
                                       A   ++  IGLREREEGDLGPVYGFQWRHFGA
Sbjct: 301 VEELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGA 360

Query: 361 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 420
           RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 361 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 420

Query: 421 NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 474
           NGELSCQMYQRSADMGL                    DLAYGDFIHVIGDAHVYRTHVRP
Sbjct: 421 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAYGDFIHVIGDAHVYRTHVRP 480

[Close]

BLAST of Cp4.1LG16g02580 vs. NCBI nr
Match: XP_022986497.1 (bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita maxima])

HSP 1 Score: 908 bits (2346), Expect = 0.0
Identity = 460/529 (86.96%), Postives = 464/529 (87.71%), Query Frame = 0


Query: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT 60
           MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT
Sbjct: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT 60

Query: 61  TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120
           TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE
Sbjct: 61  TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEI 240
           WYSSFP+VENNLRYSFTSYVRVRSSTEPP IDQNNGLVSHNKPDSFN   KNFSFLPKEI
Sbjct: 181 WYSSFPMVENNLRYSFTSYVRVRSSTEPP-IDQNNGLVSHNKPDSFN---KNFSFLPKEI 240

Query: 241 FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK------- 300
           FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK       
Sbjct: 241 FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTKKVFWRGV 300

Query: 301 ----------------------------ACARFVFCIGLREREEGDLGPVYGFQWRHFGA 360
                                       A   ++  IGLREREEGDLGPVYGFQWRHFGA
Sbjct: 301 VEELLWFISGSTNAKILQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGA 360

Query: 361 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 420
           RYTDMHADYTGQGFDQLLDVINKIKNNP+DRRIVLSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 361 RYTDMHADYTGQGFDQLLDVINKIKNNPNDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 420

Query: 421 NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 474
           NGELSCQMYQRSADMGL                    DLAYGDFIHVIGDAHVYRTHVRP
Sbjct: 421 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAYGDFIHVIGDAHVYRTHVRP 480

[Close]

BLAST of Cp4.1LG16g02580 vs. NCBI nr
Match: XP_038901596.1 (bifunctional dihydrofolate reductase-thymidylate synthase 1-like [Benincasa hispida] >XP_038901597.1 bifunctional dihydrofolate reductase-thymidylate synthase 1-like [Benincasa hispida])

HSP 1 Score: 880 bits (2273), Expect = 0.0
Identity = 438/529 (82.80%), Postives = 456/529 (86.20%), Query Frame = 0


Query: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT 60
           MA DPLTSSNG ANVNAPP+PRRTYQVVVAA+ENMGIGKDGKLPWRLPSDLKFFKNITTT
Sbjct: 1   MAGDPLTSSNGIANVNAPPDPRRTYQVVVAASENMGIGKDGKLPWRLPSDLKFFKNITTT 60

Query: 61  TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120
           TSDHGKRNA+LMGRKTWESIPAE+RPLPGRLNVVLTRSGSFDIATVENVIICGS+TSALE
Sbjct: 61  TSDHGKRNAILMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFV+GGGEILREALNAPECDA+H+TE+ETN+ECDTFIPAIDKSMFQP
Sbjct: 121 LLAASPYCLSIEKVFVVGGGEILREALNAPECDAIHITEVETNIECDTFIPAIDKSMFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEI 240
           WYSSFPVVENNLRYSFTSYVRVRSSTEPP IDQNNGLVSHNKPD  NFEAK FSFLPKEI
Sbjct: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPP-IDQNNGLVSHNKPDFSNFEAKKFSFLPKEI 240

Query: 241 FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK------- 300
           FE+HEEYLYL LVQEIIS+G  KDDRTGTGTLS+FGCQMRYNLRKTFPLLTTK       
Sbjct: 241 FEKHEEYLYLNLVQEIISNGIPKDDRTGTGTLSRFGCQMRYNLRKTFPLLTTKKVFWRGV 300

Query: 301 ----------------------------ACARFVFCIGLREREEGDLGPVYGFQWRHFGA 360
                                       A   ++  IGLREREEGDLGPVYGFQWRHFGA
Sbjct: 301 VEELLWFISGSTNANVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGA 360

Query: 361 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 420
           RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRI+LSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 361 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFAQFYVA 420

Query: 421 NGELSCQMYQRSADMGLD--------------------LAYGDFIHVIGDAHVYRTHVRP 474
           NGELSCQMYQRSADMGL                     LA GDFIHVIGDAHVYRTHVRP
Sbjct: 421 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRP 480

[Close]

BLAST of Cp4.1LG16g02580 vs. NCBI nr
Match: XP_022146106.1 (bifunctional dihydrofolate reductase-thymidylate synthase 1-like [Momordica charantia])

HSP 1 Score: 868 bits (2244), Expect = 1.43e-315
Identity = 435/529 (82.23%), Postives = 455/529 (86.01%), Query Frame = 0


Query: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT 60
           MA DPLTSSNGNANV APP+PRRT+QVVVAATENMGIGKDGKLPWRLPSDLK+FKNITTT
Sbjct: 10  MAGDPLTSSNGNANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTT 69

Query: 61  TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120
           TSDHGKRNAVLMGRKTWESIPAE+RPLPGRLNVVLTRSGSFDIATVENVIICGS+TSALE
Sbjct: 70  TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 129

Query: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVF+IGGGEILREALNAPECDA+HVT+I+T VECDTFIPAIDKSMFQP
Sbjct: 130 LLAASPYCLSIEKVFIIGGGEILREALNAPECDAIHVTDIQTCVECDTFIPAIDKSMFQP 189

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEI 240
           WYSSFPVVENNLRYSFTSYVRVRSS EPP IDQNNGLVS+++PDS NFEAK FSFLPKEI
Sbjct: 190 WYSSFPVVENNLRYSFTSYVRVRSSIEPP-IDQNNGLVSYSRPDSSNFEAKKFSFLPKEI 249

Query: 241 FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK------- 300
           FERHEEYLYL LV+EIISDGTSKDDRTGTGTLS+FG QMRYNLRKTFPLLTTK       
Sbjct: 250 FERHEEYLYLNLVKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKKVFWRGV 309

Query: 301 ----------------------------ACARFVFCIGLREREEGDLGPVYGFQWRHFGA 360
                                       A   ++  IGL EREEGDLGPVYGFQWRHFGA
Sbjct: 310 VEELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLSEREEGDLGPVYGFQWRHFGA 369

Query: 361 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 420
           RYTDMHADYTGQGFDQLLDVINKIK NP+DRRI+LSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 370 RYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPPCHMFAQFYVA 429

Query: 421 NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 474
           NGELSCQMYQRSADMGL                    DLA GDFIHVIGDAHVY THVRP
Sbjct: 430 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAQGDFIHVIGDAHVYHTHVRP 489

[Close]

BLAST of Cp4.1LG16g02580 vs. ExPASy TrEMBL
Match: A0A6J1FWP8 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucurbita moschata OX=3662 GN=LOC111448895 PE=3 SV=1)

HSP 1 Score: 919 bits (2375), Expect = 0.0
Identity = 462/529 (87.33%), Postives = 466/529 (88.09%), Query Frame = 0


Query: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT 60
           MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNIT T
Sbjct: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITAT 60

Query: 61  TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120
           TSDHGKRNA+LMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE
Sbjct: 61  TSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEI 240
           WYSSFPVVENNLRYSFTSYVRVRSSTEPP IDQNNGLVSHNKPDSFNFEAKNFSFLPKEI
Sbjct: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPP-IDQNNGLVSHNKPDSFNFEAKNFSFLPKEI 240

Query: 241 FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK------- 300
           FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLR+TFPLLTTK       
Sbjct: 241 FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTKKVFWRGV 300

Query: 301 ----------------------------ACARFVFCIGLREREEGDLGPVYGFQWRHFGA 360
                                       A   ++  IGLREREEGDLGPVYGFQWRHFGA
Sbjct: 301 VEELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGA 360

Query: 361 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 420
           RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 361 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 420

Query: 421 NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 474
           NGELSCQMYQRSADMGL                    DLAYGDFIHVIGDAHVYRTHVRP
Sbjct: 421 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAYGDFIHVIGDAHVYRTHVRP 480

[Close]

BLAST of Cp4.1LG16g02580 vs. ExPASy TrEMBL
Match: A0A6J1J7P4 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucurbita maxima OX=3661 GN=LOC111484218 PE=3 SV=1)

HSP 1 Score: 908 bits (2346), Expect = 0.0
Identity = 460/529 (86.96%), Postives = 464/529 (87.71%), Query Frame = 0


Query: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT 60
           MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT
Sbjct: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT 60

Query: 61  TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120
           TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE
Sbjct: 61  TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEI 240
           WYSSFP+VENNLRYSFTSYVRVRSSTEPP IDQNNGLVSHNKPDSFN   KNFSFLPKEI
Sbjct: 181 WYSSFPMVENNLRYSFTSYVRVRSSTEPP-IDQNNGLVSHNKPDSFN---KNFSFLPKEI 240

Query: 241 FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK------- 300
           FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK       
Sbjct: 241 FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTKKVFWRGV 300

Query: 301 ----------------------------ACARFVFCIGLREREEGDLGPVYGFQWRHFGA 360
                                       A   ++  IGLREREEGDLGPVYGFQWRHFGA
Sbjct: 301 VEELLWFISGSTNAKILQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGA 360

Query: 361 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 420
           RYTDMHADYTGQGFDQLLDVINKIKNNP+DRRIVLSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 361 RYTDMHADYTGQGFDQLLDVINKIKNNPNDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 420

Query: 421 NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 474
           NGELSCQMYQRSADMGL                    DLAYGDFIHVIGDAHVYRTHVRP
Sbjct: 421 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAYGDFIHVIGDAHVYRTHVRP 480

[Close]

BLAST of Cp4.1LG16g02580 vs. ExPASy TrEMBL
Match: A0A6J1CYC5 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Momordica charantia OX=3673 GN=LOC111015400 PE=3 SV=1)

HSP 1 Score: 868 bits (2244), Expect = 6.93e-316
Identity = 435/529 (82.23%), Postives = 455/529 (86.01%), Query Frame = 0


Query: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT 60
           MA DPLTSSNGNANV APP+PRRT+QVVVAATENMGIGKDGKLPWRLPSDLK+FKNITTT
Sbjct: 10  MAGDPLTSSNGNANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTT 69

Query: 61  TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120
           TSDHGKRNAVLMGRKTWESIPAE+RPLPGRLNVVLTRSGSFDIATVENVIICGS+TSALE
Sbjct: 70  TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 129

Query: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVF+IGGGEILREALNAPECDA+HVT+I+T VECDTFIPAIDKSMFQP
Sbjct: 130 LLAASPYCLSIEKVFIIGGGEILREALNAPECDAIHVTDIQTCVECDTFIPAIDKSMFQP 189

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEI 240
           WYSSFPVVENNLRYSFTSYVRVRSS EPP IDQNNGLVS+++PDS NFEAK FSFLPKEI
Sbjct: 190 WYSSFPVVENNLRYSFTSYVRVRSSIEPP-IDQNNGLVSYSRPDSSNFEAKKFSFLPKEI 249

Query: 241 FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK------- 300
           FERHEEYLYL LV+EIISDGTSKDDRTGTGTLS+FG QMRYNLRKTFPLLTTK       
Sbjct: 250 FERHEEYLYLNLVKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTKKVFWRGV 309

Query: 301 ----------------------------ACARFVFCIGLREREEGDLGPVYGFQWRHFGA 360
                                       A   ++  IGL EREEGDLGPVYGFQWRHFGA
Sbjct: 310 VEELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLSEREEGDLGPVYGFQWRHFGA 369

Query: 361 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 420
           RYTDMHADYTGQGFDQLLDVINKIK NP+DRRI+LSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 370 RYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPPCHMFAQFYVA 429

Query: 421 NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 474
           NGELSCQMYQRSADMGL                    DLA GDFIHVIGDAHVY THVRP
Sbjct: 430 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAQGDFIHVIGDAHVYHTHVRP 489

[Close]

BLAST of Cp4.1LG16g02580 vs. ExPASy TrEMBL
Match: A0A0A0LPA5 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis sativus OX=3659 GN=Csa_2G248730 PE=3 SV=1)

HSP 1 Score: 860 bits (2223), Expect = 5.55e-313
Identity = 434/529 (82.04%), Postives = 451/529 (85.26%), Query Frame = 0


Query: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT 60
           MAADPLTSSNG ANVN+PP+PRRTYQVVVAATEN+GIGK GKLPWRLPSD+KFFKNIT T
Sbjct: 1   MAADPLTSSNGIANVNSPPDPRRTYQVVVAATENLGIGKGGKLPWRLPSDIKFFKNITIT 60

Query: 61  TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120
           TSD GKRNAVLMGRKTWES+PAE+RPLPGRLNVVLTRSGSFDIATVENVIICGS+TSALE
Sbjct: 61  TSDPGKRNAVLMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREA+NAPECDAVH+TEIETNVECDTFIPA+DKS+FQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNVECDTFIPAVDKSIFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEI 240
           WYSSFPVVE NLRYSF SYVRVRSS EPP IDQNNGLVSHNKPD  NFEAK FSFLPKEI
Sbjct: 181 WYSSFPVVEKNLRYSFNSYVRVRSSAEPP-IDQNNGLVSHNKPDFSNFEAK-FSFLPKEI 240

Query: 241 FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK------- 300
           FERHEEYLYLKLVQEIISDG  KDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK       
Sbjct: 241 FERHEEYLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTKKVFWRGV 300

Query: 301 ----------------------------ACARFVFCIGLREREEGDLGPVYGFQWRHFGA 360
                                       A   ++  IGLREREEGDLGPVYGFQWRHFGA
Sbjct: 301 VEELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGA 360

Query: 361 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 420
           RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRI+LSAWNPSDL+LMALPPCHMF+QFYVA
Sbjct: 361 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLQLMALPPCHMFSQFYVA 420

Query: 421 NGELSCQMYQRSADMGLD--------------------LAYGDFIHVIGDAHVYRTHVRP 474
           NGELSCQMYQRSADMGL                     LA GDFIHVIGDAHVYRTHVRP
Sbjct: 421 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRP 480

[Close]

BLAST of Cp4.1LG16g02580 vs. ExPASy TrEMBL
Match: A0A1S3CE37 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis melo OX=3656 GN=LOC103499691 PE=3 SV=1)

HSP 1 Score: 860 bits (2222), Expect = 8.18e-313
Identity = 433/529 (81.85%), Postives = 449/529 (84.88%), Query Frame = 0


Query: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT 60
           MAADPLT SNG ANVN+ P+PRRTYQVVVAATEN+GIGKDGKLPWRLPSDLKFFKNITTT
Sbjct: 1   MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT 60

Query: 61  TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120
           TSD GKRNA+LMGRKTWES+PAE+RPLPGRLNVVLTRSGSFDIATVENVIICGS+ SALE
Sbjct: 61  TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREA+NAPECDAVH+TEIETN+ECDTFIPAIDK+MFQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKAMFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEI 240
           WYSSFPVVENNLRYSF SYVRVRSS E P IDQNNGLVSHNKPD  N EAK FSFLPKEI
Sbjct: 181 WYSSFPVVENNLRYSFNSYVRVRSSAELP-IDQNNGLVSHNKPDFSNIEAKKFSFLPKEI 240

Query: 241 FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK------- 300
           FERHEEYLYLKLVQEIISDG  KDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK       
Sbjct: 241 FERHEEYLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTKKVFWRGV 300

Query: 301 ----------------------------ACARFVFCIGLREREEGDLGPVYGFQWRHFGA 360
                                       A   ++  IGLREREEGDLGPVYGFQWRHFGA
Sbjct: 301 VEELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGA 360

Query: 361 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 420
           RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRI+LSAWNPSDLKLMALPPCHMF+QFYVA
Sbjct: 361 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVA 420

Query: 421 NGELSCQMYQRSADMGLD--------------------LAYGDFIHVIGDAHVYRTHVRP 474
           NGELSCQMYQRSADMGL                     LA GDFIHVIGDAHVYRTHVRP
Sbjct: 421 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRP 480

[Close]

BLAST of Cp4.1LG16g02580 vs. TAIR 10
Match: AT2G16370.1 (thymidylate synthase 1 )

HSP 1 Score: 702.6 bits (1812), Expect = 2.2e-202
Identity = 346/523 (66.16%), Postives = 401/523 (76.67%), Query Frame = 0


Query: 7   TSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTTTSDHGK 66
           T+ N +        P+RTYQVVVAAT+ MGIGKDGKLPW LP+DLKFFK+IT TTSD  K
Sbjct: 4   TTLNDSVTTTLASEPQRTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDITLTTSDSSK 63

Query: 67  RNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALELLAASP 126
           +NAV+MGRKTWESIP +YRPL GRLNVVLTRSG FDIA  ENV+ C S+ SAL+LLAA P
Sbjct: 64  KNAVVMGRKTWESIPIKYRPLSGRLNVVLTRSGGFDIANTENVVTCSSVDSALDLLAAPP 123

Query: 127 YCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQPWYSSFP 186
           YCLSIE+VFVIGGG+ILREALN P CDA+H+TEI+T+V+CDTFIPAID S++QPW SSFP
Sbjct: 124 YCLSIERVFVIGGGDILREALNRPSCDAIHLTEIDTSVDCDTFIPAIDTSVYQPWSSSFP 183

Query: 187 VVENNLRYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIFERHEE 246
           V EN LR+ FT++VRV+SS +    D++NG        S  F+ K F FLPK +F++HEE
Sbjct: 184 VTENGLRFCFTTFVRVKSSADESS-DESNG------SQSLQFDGKKFLFLPKMVFDQHEE 243

Query: 247 YLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK------------- 306
           +LYL +V++IIS+G  K+DRTGTGTLSKFGCQM++NLR++FPLLTTK             
Sbjct: 244 FLYLNMVEDIISNGNVKNDRTGTGTLSKFGCQMKFNLRRSFPLLTTKRVFWRGVVEELLW 303

Query: 307 ----------------------ACARFVFCIGLREREEGDLGPVYGFQWRHFGARYTDMH 366
                                 A   ++  IGL EREEGDLGPVYGFQWRHFGA+YTDMH
Sbjct: 304 FISGSTNAKVLQEKGIHIWDGNASREYLDGIGLTEREEGDLGPVYGFQWRHFGAKYTDMH 363

Query: 367 ADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVANGELSC 426
           ADYTGQGFDQL+DVI+KIKNNPDDRRI++SAWNPSDLKLMALPPCHMFAQFYVA GELSC
Sbjct: 364 ADYTGQGFDQLVDVIDKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFAQFYVAEGELSC 423

Query: 427 QMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRPLQEQLQ 475
           QMYQRSADMGL                    DL  GDFIHV+GDAHVY+THVRPLQEQL 
Sbjct: 424 QMYQRSADMGLGVPFNIASYSLLTCMLAHVCDLVPGDFIHVLGDAHVYKTHVRPLQEQLL 483

[Close]

BLAST of Cp4.1LG16g02580 vs. TAIR 10
Match: AT4G34570.1 (thymidylate synthase 2 )

HSP 1 Score: 687.6 bits (1773), Expect = 7.2e-198
Identity = 344/519 (66.28%), Postives = 392/519 (75.53%), Query Frame = 0


Query: 12  NANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTTTSDHGKRNAVL 71
           N NV     P+ TYQVVVAAT+ MGIGKDGKLPW LP+DLKFFK++T +TSD  K+NAV+
Sbjct: 53  NGNVIMTSKPQSTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDLTLSTSDSAKKNAVV 112

Query: 72  MGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALELLAASPYCLSI 131
           MGRKTWESIP +YRPL GRLNVVL+RS  FDIA  ENV+ C S+ SAL+LLAA P+ LSI
Sbjct: 113 MGRKTWESIPKKYRPLSGRLNVVLSRSSGFDIANTENVVTCSSIDSALDLLAAPPFSLSI 172

Query: 132 EKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQPWYSSFPVVENN 191
           EKVFVIGGG+ILREALN P C+A+H+TEI+T+++CDTFIP +D S +QPW SSFP+ EN 
Sbjct: 173 EKVFVIGGGDILREALNKPSCEAIHITEIDTSIDCDTFIPTVDTSAYQPWCSSFPICENG 232

Query: 192 LRYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFNFEAKNF-SFLPKEIFERHEEYLYL 251
           LR+SFT++VRV+SS+     D+++G            + K F S LPK IF+RHEEYLYL
Sbjct: 233 LRFSFTTHVRVKSSSAGEASDESDG------SKVLQVDWKKFSSVLPKMIFDRHEEYLYL 292

Query: 252 KLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK----------------- 311
            LV+EIIS+G  KDDRTGTGTLSKFGCQM++NLR+ FPLLTTK                 
Sbjct: 293 NLVKEIISNGNLKDDRTGTGTLSKFGCQMKFNLRRNFPLLTTKRVFWRGVVEELLWFISG 352

Query: 312 ------------------ACARFVFCIGLREREEGDLGPVYGFQWRHFGARYTDMHADYT 371
                             A   ++  IGL EREEGDLGPVYGFQWRHFGA+YTDMHADYT
Sbjct: 353 STNAKVLQEKGIRIWDGNASRAYLDGIGLTEREEGDLGPVYGFQWRHFGAKYTDMHADYT 412

Query: 372 GQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQ 431
           GQGFDQLLDVINKIKNNPDDRRI++SAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQ
Sbjct: 413 GQGFDQLLDVINKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQ 472

Query: 432 RSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRPLQEQLQKLPK 475
           RSADMGL                    DL  GDFIHVIGDAHVY+ HVRPLQEQL+  PK
Sbjct: 473 RSADMGLGVPFNIASYSLLTCILAHVCDLVPGDFIHVIGDAHVYKNHVRPLQEQLENPPK 532

[Close]

BLAST of Cp4.1LG16g02580 vs. TAIR 10
Match: AT2G21550.1 (Bifunctional dihydrofolate reductase/thymidylate synthase )

HSP 1 Score: 471.5 bits (1212), Expect = 8.1e-133
Identity = 254/508 (50.00%), Postives = 321/508 (63.19%), Query Frame = 0


Query: 9   SNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTTTSDHGKRN 68
           +NG + V+     RR+YQVV+AAT +MG+G D KLPW LPS+ +FF+++TT TSD  KRN
Sbjct: 10  NNGQSAVSV--TRRRSYQVVIAATRDMGLGMDMKLPWDLPSEYQFFQDVTTRTSDPTKRN 69

Query: 69  AVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALELLAASPYC 128
           A +MGRK+WES P E RPLPGRLN+VLT+S   +IA  ENV++  SM SALELLA  PY 
Sbjct: 70  ATIMGRKSWESTPLEIRPLPGRLNIVLTKSSCHNIAIDENVLVSSSMESALELLATEPYS 129

Query: 129 LSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQPWYSSFPVV 188
           LSIEKVFVIGGGE+LR  +NA  CDA+H+TEI+ +V CD F P +D S+++PWYSSFPVV
Sbjct: 130 LSIEKVFVIGGGELLRNYMNASICDAIHLTEIDISVPCDAFAPRVDTSLYRPWYSSFPVV 189

Query: 189 ENNLRYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIFERHEEYL 248
           EN +RYSF +YVR + +           + S  K      + K +SFLPK +FERHEE+ 
Sbjct: 190 ENGIRYSFNTYVRRKDAI----------VGSGEKKSVAESDLKEYSFLPKMVFERHEEFG 249

Query: 249 YLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTKACARFVFCIGLRE-- 308
           YL LVQ IIS G   D+     TLSKFGCQMR+NLRKTFPLLTTK     +F +G+ E  
Sbjct: 250 YLNLVQNIISSGDMNDN----STLSKFGCQMRFNLRKTFPLLTTKK----IFWLGVVEEI 309

Query: 309 -----------------------------------REEGDLGPVYGFQWRHFGARYTDMH 368
                                               E+GD   ++G  W+H  AR+    
Sbjct: 310 LQLISGSNNPKENGSHIWDTDEAKEYLDSFGVNATEEDGDNPFLHGLHWKHCDARFVI-- 369

Query: 369 ADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVANGELSC 428
                Q F QL DVINKIKNNP D+RI+L+A NP D KL ++ PCH F QFYVANGE+SC
Sbjct: 370 -----QEFSQLSDVINKIKNNPHDQRIMLAACNPLDFKL-SVSPCHTFTQFYVANGEVSC 429

Query: 429 QMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRPLQEQLQ 460
           Q+YQ S +  +                    DL  GDFIHVIG A++ + HV+ +Q+QLQ
Sbjct: 430 QIYQSSTEASIGIPFSIATYSLLTCIIAHVCDLGAGDFIHVIGQAYINKAHVKAIQKQLQ 489

The following BLAST results are available for this feature:

BLAST of Cp4.1LG16g02580 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P51820	4.9e-207	68.17	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Glycine max OX=3847...	[more]
P45350	4.7e-202	66.60	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Daucus carota OX=40...	[more]
Q05762	3.1e-201	66.16	Bifunctional dihydrofolate reductase-thymidylate synthase 1 OS=Arabidopsis thali...	[more]
O81395	4.6e-197	64.74	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Zea mays OX=4577 GN...	[more]
Q05763	1.0e-196	66.28	Bifunctional dihydrofolate reductase-thymidylate synthase 2 OS=Arabidopsis thali...	[more]

BLAST of Cp4.1LG16g02580 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023513067.1	0.0	88.28	bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita pepo s...	[more]
XP_022944443.1	0.0	87.33	bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita moscha...	[more]
XP_022986497.1	0.0	86.96	bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita maxima...	[more]
XP_038901596.1	0.0	82.80	bifunctional dihydrofolate reductase-thymidylate synthase 1-like [Benincasa hisp...	[more]
XP_022146106.1	1.43e-315	82.23	bifunctional dihydrofolate reductase-thymidylate synthase 1-like [Momordica char...	[more]

BLAST of Cp4.1LG16g02580 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FWP8	0.0	87.33	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucurbita moschata ...	[more]
A0A6J1J7P4	0.0	86.96	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucurbita maxima OX...	[more]
A0A6J1CYC5	6.93e-316	82.23	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Momordica charantia...	[more]
A0A0A0LPA5	5.55e-313	82.04	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis sativus OX=...	[more]
A0A1S3CE37	8.18e-313	81.85	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis melo OX=365...	[more]

BLAST of Cp4.1LG16g02580 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G16370.1	2.2e-202	66.16	thymidylate synthase 1	[more]
AT4G34570.1	7.2e-198	66.28	thymidylate synthase 2	[more]
AT2G21550.1	8.1e-133	50.00	Bifunctional dihydrofolate reductase/thymidylate synthase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000398	Thymidylate synthase	PRINTS	PR00108	THYMDSNTHASE	coord: 345..364 score: 60.96 coord: 371..386 score: 73.54 coord: 389..415 score: 41.77
IPR000398	Thymidylate synthase	TIGRFAM	TIGR03284	TIGR03284	coord: 307..403 e-value: 8.8E-36 score: 121.5
IPR001796	Dihydrofolate reductase domain	PFAM	PF00186	DHFR_1	coord: 26..201 e-value: 1.4E-38 score: 132.2
IPR001796	Dihydrofolate reductase domain	PROSITE	PS51330	DHFR_2	coord: 24..201 score: 53.062851
IPR001796	Dihydrofolate reductase domain	CDD	cd00209	DHFR	coord: 28..200 e-value: 2.59443E-62 score: 198.131
IPR024072	Dihydrofolate reductase-like domain superfamily	GENE3D	3.40.430.10	Dihydrofolate Reductase, subunit A	coord: 8..203 e-value: 3.6E-64 score: 218.3
IPR024072	Dihydrofolate reductase-like domain superfamily	SUPERFAMILY	53597	Dihydrofolate reductase-like	coord: 20..201
IPR036926	Thymidylate synthase/dCMP hydroxymethylase superfamily	GENE3D	3.30.572.10	Thymidylate synthase/dCMP hydroxymethylase domain	coord: 234..298 e-value: 1.5E-16 score: 61.9
IPR036926	Thymidylate synthase/dCMP hydroxymethylase superfamily	GENE3D	3.30.572.10	Thymidylate synthase/dCMP hydroxymethylase domain	coord: 299..474 e-value: 7.4E-74 score: 250.2
IPR036926	Thymidylate synthase/dCMP hydroxymethylase superfamily	SUPERFAMILY	55831	Thymidylate synthase/dCMP hydroxymethylase	coord: 228..474
IPR023451	Thymidylate synthase/dCMP hydroxymethylase domain	PFAM	PF00303	Thymidylat_synt	coord: 249..295 e-value: 2.5E-13 score: 49.7 coord: 403..474 e-value: 3.4E-19 score: 69.0 coord: 307..405 e-value: 1.1E-36 score: 126.4
IPR023451	Thymidylate synthase/dCMP hydroxymethylase domain	CDD	cd00351	TS_Pyrimidine_HMase	coord: 249..427 e-value: 1.58076E-76 score: 236.789
IPR012262	Bifunctional dihydrofolate reductase/thymidylate synthase	PIRSF	PIRSF000389	DHFR-TS	coord: 9..301 e-value: 4.9E-104 score: 346.6 coord: 294..406 e-value: 8.0E-62 score: 207.3 coord: 402..474 e-value: 3.3E-28 score: 96.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..21
None	No IPR available	PANTHER	PTHR11548:SF12	BIFUNCTIONAL DIHYDROFOLATE REDUCTASE-THYMIDYLATE SYNTHASE	coord: 403..474 coord: 1..294 coord: 295..404
IPR045097	Thymidylate synthase/dCMP hydroxymethylase	PANTHER	PTHR11548	THYMIDYLATE SYNTHASE 1	coord: 403..474 coord: 1..294 coord: 295..404
IPR017925	Dihydrofolate reductase conserved site	PROSITE	PS00075	DHFR_1	coord: 36..58
IPR020940	Thymidylate synthase, active site	PROSITE	PS00091	THYMIDYLATE_SYNTHASE	coord: 356..384

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG16g02580.1	Cp4.1LG16g02580.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006231	dTMP biosynthetic process
biological_process	GO:0032259	methylation
biological_process	GO:0006730	one-carbon metabolic process
biological_process	GO:0046654	tetrahydrofolate biosynthetic process
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0004146	dihydrofolate reductase activity
molecular_function	GO:0004799	thymidylate synthase activity
molecular_function	GO:0016741	transferase activity, transferring one-carbon groups