Cp4.1LG16g02410 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG16g02410
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionGlucan endo-1,3-beta-D-glucosidase
LocationCp4.1LG16: 4447955 .. 4452260 (-)
RNA-Seq ExpressionCp4.1LG16g02410
SyntenyCp4.1LG16g02410
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACTATAGTGGTGGGCCTCCCTGTTGAAAAGGGCGTAGTCTTTTTTTCCAAAGCTTCCAATTTTGCCCCTCTCTTCTGGGCCCCACCTTCCCCCCATCTCTGAACTTGCGGACTTTCACACCTCACATTCACATAACAAACACAGAGAGAGACGGAGAGAGAGACAGAGAGACAGAGAGACAGGCGGAGAGAAATACAGAGACCTCTCTCTATCTCTCTCTCTCTGCAATTCTGCGTTCATTTCATGTCCGGCTGATATTTTTCGTTCTGTTGTTAATCATGGCCGCCTCCTCCTTTTCACTCACTTTCGTCTCCTTCTCTCTCTTCGCCGCCTTTTACCTCGCCGGTCTCTTTCTCTACTCCCTTTTTCCTTTTCTCTTTTCACTCATTTTCTTTTCTCAATTCTTTTGTTTCTTTGTTTCTCTTTTGCAGACTCTCAATCCTTCATCGGCGTCAACTATGGCCAAGTCGCCAACAACCTTCCGCCGCCGGTTTCCACTGCCGCACTACTTCGCTCAACTACAATCGGTAAAGTCCGACTCTACGGCGCCGATCCACAAATAATCAAAGCCCTAGCCAACACCGGAATAGGAATCGTGATTGGCGTAGCCAATGGCGACATTCCTGCCCTGGCCTCGAATCCGAACTCCGCTGCTCAATGGATCAACACGAATGTCGTTCCCTACTATCCAGCCAGCAATATCGTGCTGATTACCGTCGGCAACGAGGTGATTTCATCCATGGATCAAGGCTTGGTTTCGCAGCTTCTCCCAGCGATGCAGAACGTGCAGAACGCTCTGAATTCGGCGAACCTTGGCGGTAAGGTGAAGGTGTCGACAGTGCACTCGATGGCGGTGTTAAGCCAGTCGGATCCGCCGTCGTCGGGGAGGTTCAATCCGGTGTTGGAACAGATGATGAAGGCGGTGGTGGAATTCGAGAAGGAGAACGCGTCGCCATTTGCGATAAACCCTTACCCGTTCTTCGCGTACCAGAGCGATCCGAGAGCCGAAACGCTGGCGTTTTGCCTGTTTCAACCCAACTCGGGCCGGGTCGACTCGGGAACCGGAATAAAGTACATGAACATGTTCGATGCTCAGGTCAGTCAACCGCTCCAATCAATTTCTCCTAATTTCGTTTCAATTTCTTAATCTTAATTAATTAATTAATTAATTCCAATTCTTTCACCTTAAACACCGACGACGTTTACTTTAAGTCAACAGTCAATAGCCTCAGATTTTGACACGTGTCAATAATATTTAAATTTATTATTATTTTTTTTTACCTTTTTTTTTTAAACATTATAAATATAAAGGTTAGGAGTAAATATGTAACTTAATTTTTTTTTTTAAGTATATATAATTTGAAGATTAATTTAACGTCACTTTTTTTTGGGGGTTGCTGATGGTTATATTTTATTCTTTTGTGGTTAAAAAATCTCTTTCCATATGCATCGAAATTAGCAAATTTATCATTTTCAGAATCAATTTTTCAGAAAGTAATCTGAATGACGTTATTTATCAATTTGGGTTATTTTTATCCTGGCTGCTAGATTTGGAATTTGAATACTTTTTAAAGATCTTGTTGCTATTTACAGTTCTTGTGGTGTATTTTGATTTGGTTCTTGCTTGTGGGTACTTCGCGTAAATTGCTTGAAACTAAACAATGGGTGTAGATAATGATGTGTTTGAAGTTTTGATTGTTTCTGCCTGCAAGGTGTTTGTGATAATGCGTGAAAGAAATTGATGTGTGAAATCACTCGCTTGTTTAATCGCCCTTTCCTGATTTTACACCCAATTGAAGACTAAAAGCCTAATATGCTGTTGCATTTTCAATCCAAAAGGCCAATTTAGAAGAAAAAAGTGATCAGTTGGGAGATCTTTTGATTGTTTGTGTCTGCAAGGTGTTTGTGAGATTGCGTGAAAGAAATTGATGTATGAAATCAGTAACCACTCGCTTGCTTAAACGCCCCTTCCTGATTTTACACCCAATTGAAGACTAAAAGCCTGATATTCTGTTCAACCCAAAAGGCCAAAAGAATTTAGAAAAAAGTGATGAGGTGGGAGATGTCATTCAACTTTCGAATCTTAGCAAATAAGCTTGCTTTTCCTGTGTGATGTGACCTATATTATGCTCGGTTACAGCTGGCCTGTGTCATTTAATGTCGGTAAGTCCTTCCAAGATATCAATTATTATTAAGAGGAATGCCTACATATTATTTATTGGCCTGGAATTTTTTCTTCCTTTTCTTTTTGGGTTTATTGAGCTGTGATATTGGTAAGAGCTAGTGTCCTCGTGACAAGGATTAGGGACGTTTGAAGACACATTTCAAAATTTTGTATTCAAGCCCTAGCCCCTCCATGAATGGATGGAGCGGATTAGAGATAACCCTACAATTGGTTAAAATGGTTAAAAAGTCTTTTGACATAATATTGCAATAAAAAGGAAAAGAAAAGGTTTTGTCTCCCTCTCCTCTTCTCCTGTCCCTACTTACTCCTGACTCTCCTCGCCCTTTTTCTTCTTTTTCGATATGCGGCTCTGTGAGAAAGAAAATTATCTCCTGTCCCTACTTACTCCTGACTCTCCTCGCCCTTTTTCTTCTTTTTCGATATGCGGCTCTGTGAGAAAGAAAATTAAGTGGGCTGCAGTGGTGTAAGAATTTGGTTTTTCTACTTTCACTTTTTTGTGAGAAAAGATATTGGGGAGACCCTTGACTTGTGCGGGCTCTCACGCCCCTTCTTTATTTAGTTTGTTTATTTCGGTGGTGTCTCGAGTCAGGTTGGGGACTATTTTGTATTCAAGATTTAAATTTTTTACAACTTCAGAACTCTTGGTTTTGGTGTGGGTGCTCATAGAAAAAAATTATGATTATCAAGGGAGCACAGCATATGGTGGTGCTGGAATCCCCCCAACAACCAAACAAGCACATTTTGAATTTTGTGGCATTTCTTTTTGTGGTCTTCAACTACCATGTTTGTTTAAAACTTGTATTTATGATATCTTGCAACAGATTCTAATAATTTGAACTACTACATCCATTATTGGGAGCTATTATTATTTGGTCCATGACTCATATTGATTTCAACAGCTTGATGCAGTAAGGTCTGCCTTGAATGCACTGGGTGATTTCAAGGATGTCGAGATCTTGGTAGCCGAGACGGGGTGGGCGTATCGCGGTGATAGCAATGAAGTTGGAACAACTATGGAAAATGCAAGAGCTTACAATGGCAATTTGATTGCACATCTTAGATCAATGGTGGGTACTCCATTGATGCCTGGAAAATCAGTGGATACATATATCTTTGCTCTCTATGATGAAAACTTGAAGCCTGGCCCCACCTCCGAACGATCATTTGGGCTGTTCCGTCCTGATCTAACCATGGCATATGATGTTGGGCTCTCAAAGAACAGCCAGGTCAGTCAATGGTTGAATCATTGATTGCTTTTGATAAAATAAGCTTCTTGCAGATAACAAATTGCATCAACTTTACTTTGATAAACTTTATCCAATCATTTTACATCCATGAAGTTGACTCTTGAACGTTACAGACTCCTACAACTCCAACCACCCCGTCCCCAAAACCGACTGCGCTTGCTTGGTGCATGCCCAAGGCAGGTGCTTCTGATGCTCAACTTCAGTTGAATCTCGACTATGCTTGTGGGCACGGTATCGATTGCGGGGCAATCCAACCGGGGGGTGCCTGTTTTGAGCCAAACACTGTAAAATCACATGCTGCTTATGCTATGAATCTCTACTACCAATCTCTTGGCAAGAATCCATGGGACTGTGATTTCTCGCAGACAGCAACACTAACATCCACAAATCCTAGTAAGCTTTCTATTCTAATATCTCTTCCATTCAATTTCAGCCAAGCAATAAGGGAAGGACAGAAAGTTATCATATTTTTGGTGTTATTGCTTTAGTTGAAGTTCTTACATTTGTGGATTTTCAGGTTATAATGGCTGCTCGTATCCAGGCGGAAGCAGCTGAGAAGGGAGATCATAGTGCAGTGATATGAGATAAAGAATTAGTTTTCCATATTCTAAGGTGTCTTTAAGACATTTGAAAACAAACGAATGAAGCAATAATCTAATTAATTGGCCAACAATTGGTCATTCTTAGTTGCCCTGTCCAACATTCTACAAAGCCAAAATGGCTACCTTCCTAAATTCTATTAATTAAGAATAACAATTTTTTTAGGGGAAAAATCAATCCATGCCTTAATTTGCTTGGTATATTAATTTGAACTTCAACTTTTACCNGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTAATTTCAACC

mRNA sequence

AACTATAGTGGTGGGCCTCCCTGTTGAAAAGGGCGTAGTCTTTTTTTCCAAAGCTTCCAATTTTGCCCCTCTCTTCTGGGCCCCACCTTCCCCCCATCTCTGAACTTGCGGACTTTCACACCTCACATTCACATAACAAACACAGAGAGAGACGGAGAGAGAGACAGAGAGACAGAGAGACAGGCGGAGAGAAATACAGAGACCTCTCTCTATCTCTCTCTCTCTGCAATTCTGCGTTCATTTCATGTCCGGCTGATATTTTTCGTTCTGTTGTTAATCATGGCCGCCTCCTCCTTTTCACTCACTTTCGTCTCCTTCTCTCTCTTCGCCGCCTTTTACCTCGCCGACTCTCAATCCTTCATCGGCGTCAACTATGGCCAAGTCGCCAACAACCTTCCGCCGCCGGTTTCCACTGCCGCACTACTTCGCTCAACTACAATCGGTAAAGTCCGACTCTACGGCGCCGATCCACAAATAATCAAAGCCCTAGCCAACACCGGAATAGGAATCGTGATTGGCGTAGCCAATGGCGACATTCCTGCCCTGGCCTCGAATCCGAACTCCGCTGCTCAATGGATCAACACGAATGTCGTTCCCTACTATCCAGCCAGCAATATCGTGCTGATTACCGTCGGCAACGAGGTGATTTCATCCATGGATCAAGGCTTGGTTTCGCAGCTTCTCCCAGCGATGCAGAACGTGCAGAACGCTCTGAATTCGGCGAACCTTGGCGGTAAGGTGAAGGTGTCGACAGTGCACTCGATGGCGGTGTTAAGCCAGTCGGATCCGCCGTCGTCGGGGAGGTTCAATCCGGTGTTGGAACAGATGATGAAGGCGGTGGTGGAATTCGAGAAGGAGAACGCGTCGCCATTTGCGATAAACCCTTACCCGTTCTTCGCGTACCAGAGCGATCCGAGAGCCGAAACGCTGGCGTTTTGCCTGTTTCAACCCAACTCGGGCCGGGTCGACTCGGGAACCGGAATAAAGTACATGAACATGTTCGATGCTCAGCTTGATGCAGTAAGGTCTGCCTTGAATGCACTGGGTGATTTCAAGGATGTCGAGATCTTGGTAGCCGAGACGGGGTGGGCGTATCGCGGTGATAGCAATGAAGTTGGAACAACTATGGAAAATGCAAGAGCTTACAATGGCAATTTGATTGCACATCTTAGATCAATGGTGGGTACTCCATTGATGCCTGGAAAATCAGTGGATACATATATCTTTGCTCTCTATGATGAAAACTTGAAGCCTGGCCCCACCTCCGAACGATCATTTGGGCTGTTCCGTCCTGATCTAACCATGGCATATGATGTTGGGCTCTCAAAGAACAGCCAGACTCCTACAACTCCAACCACCCCGTCCCCAAAACCGACTGCGCTTGCTTGGTGCATGCCCAAGGCAGGTGCTTCTGATGCTCAACTTCAGTTGAATCTCGACTATGCTTGTGGGCACGGTATCGATTGCGGGGCAATCCAACCGGGGGGTGCCTGTTTTGAGCCAAACACTGTAAAATCACATGCTGCTTATGCTATGAATCTCTACTACCAATCTCTTGGCAAGAATCCATGGGACTGTGATTTCTCGCAGACAGCAACACTAACATCCACAAATCCTAGTTATAATGGCTGCTCGTATCCAGGCGGAAGCAGCTGAGAAGGGAGATCATAGTGCAGTGATATGAGATAAAGAATTAGTTTTCCATATTCTAAGGTGTCTTTAAGACATTTGAAAACAAACGAATGAAGCAATAATCTAATTAATTGGCCAACAATTGGTCATTCTTAGTTGCCCTGTCCAACATTCTACAAAGCCAAAATGGCTACCTTCCTAAATTCTATTAATTAAGAATAACAATTTTTTTAGGGGAAAAATCAATCCATGCCTTAATTTGCTTGGTATATTAATTTGAACTTCAACTTTTACCNGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTAATTTCAACC

Coding sequence (CDS)

ATGGCCGCCTCCTCCTTTTCACTCACTTTCGTCTCCTTCTCTCTCTTCGCCGCCTTTTACCTCGCCGACTCTCAATCCTTCATCGGCGTCAACTATGGCCAAGTCGCCAACAACCTTCCGCCGCCGGTTTCCACTGCCGCACTACTTCGCTCAACTACAATCGGTAAAGTCCGACTCTACGGCGCCGATCCACAAATAATCAAAGCCCTAGCCAACACCGGAATAGGAATCGTGATTGGCGTAGCCAATGGCGACATTCCTGCCCTGGCCTCGAATCCGAACTCCGCTGCTCAATGGATCAACACGAATGTCGTTCCCTACTATCCAGCCAGCAATATCGTGCTGATTACCGTCGGCAACGAGGTGATTTCATCCATGGATCAAGGCTTGGTTTCGCAGCTTCTCCCAGCGATGCAGAACGTGCAGAACGCTCTGAATTCGGCGAACCTTGGCGGTAAGGTGAAGGTGTCGACAGTGCACTCGATGGCGGTGTTAAGCCAGTCGGATCCGCCGTCGTCGGGGAGGTTCAATCCGGTGTTGGAACAGATGATGAAGGCGGTGGTGGAATTCGAGAAGGAGAACGCGTCGCCATTTGCGATAAACCCTTACCCGTTCTTCGCGTACCAGAGCGATCCGAGAGCCGAAACGCTGGCGTTTTGCCTGTTTCAACCCAACTCGGGCCGGGTCGACTCGGGAACCGGAATAAAGTACATGAACATGTTCGATGCTCAGCTTGATGCAGTAAGGTCTGCCTTGAATGCACTGGGTGATTTCAAGGATGTCGAGATCTTGGTAGCCGAGACGGGGTGGGCGTATCGCGGTGATAGCAATGAAGTTGGAACAACTATGGAAAATGCAAGAGCTTACAATGGCAATTTGATTGCACATCTTAGATCAATGGTGGGTACTCCATTGATGCCTGGAAAATCAGTGGATACATATATCTTTGCTCTCTATGATGAAAACTTGAAGCCTGGCCCCACCTCCGAACGATCATTTGGGCTGTTCCGTCCTGATCTAACCATGGCATATGATGTTGGGCTCTCAAAGAACAGCCAGACTCCTACAACTCCAACCACCCCGTCCCCAAAACCGACTGCGCTTGCTTGGTGCATGCCCAAGGCAGGTGCTTCTGATGCTCAACTTCAGTTGAATCTCGACTATGCTTGTGGGCACGGTATCGATTGCGGGGCAATCCAACCGGGGGGTGCCTGTTTTGAGCCAAACACTGTAAAATCACATGCTGCTTATGCTATGAATCTCTACTACCAATCTCTTGGCAAGAATCCATGGGACTGTGATTTCTCGCAGACAGCAACACTAACATCCACAAATCCTAGTTATAATGGCTGCTCGTATCCAGGCGGAAGCAGCTGA

Protein sequence

MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLYGADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGNEVISSMDQGLVSQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVLEQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNMFDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSMVGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMAYDVGLSKNSQTPTTPTTPSPKPTALAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAYAMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS
Homology
BLAST of Cp4.1LG16g02410 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 634.8 bits (1636), Expect = 7.5e-181
Identity = 314/454 (69.16%), Postives = 376/454 (82.82%), Query Frame = 0

Query: 3   ASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLYGA 62
           A S S+ F+   L + F  + ++ FIGVNYGQVA+NLPPP  T  LL+ST+I KVRLYGA
Sbjct: 2   ALSISIYFLLIFL-SHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGA 61

Query: 63  DPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGNEV 122
           DP IIKALA TG+GIVIG ANGD+P+LAS+PN+A QWIN+NV+P+YPAS I+LITVGNE+
Sbjct: 62  DPAIIKALAGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEI 121

Query: 123 ISSMDQGLVSQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVLEQ 182
           + S D  LV+QLLPAMQNVQ AL + +LGGK+KVSTV+SM VL  SDPPSSG F    + 
Sbjct: 122 LMSNDPNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQT 181

Query: 183 MMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNMFD 242
            +K +++F  +  SPFAINPYPFFAYQSDPR ETLAFCLF+PN+GRVDS TGIKY NMFD
Sbjct: 182 GLKGILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFD 241

Query: 243 AQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSMVG 302
           AQ+DAV SAL ++G F+ VEI+VAETGWA RGD+NEVG +++NA+AYNGNLIAHLRSMVG
Sbjct: 242 AQVDAVHSALKSMG-FEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVG 301

Query: 303 TPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMAYDVGLSKNSQTPTTPTTPS 362
           TPLMPGK VDTYIFALYDENLKPGP+SER+FGLF+ DL+M YDVGL+K+S   ++  TPS
Sbjct: 302 TPLMPGKPVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLAKSS---SSSQTPS 361

Query: 363 PKPTALAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAYAMNLY 422
            K T+  WC+PK GA++ +LQ +LD+ACGHGIDCGAIQPGGACFEPN V SHAAYAMN+Y
Sbjct: 362 GKVTSSGWCVPKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMY 421

Query: 423 YQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGG 457
           +Q   K P DCDFS+TAT+TS NPSYN C YPGG
Sbjct: 422 FQKSPKQPTDCDFSKTATVTSQNPSYNNCVYPGG 450

BLAST of Cp4.1LG16g02410 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 1.3e-180
Identity = 309/453 (68.21%), Postives = 377/453 (83.22%), Query Frame = 0

Query: 7   SLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLYGADPQI 66
           SL F+ F L   FY A+SQSF+GVNYGQ+++NLP   +T  LL+STTI KVRL+GA+P +
Sbjct: 9   SLLFLVFLLLQNFYSANSQSFLGVNYGQLSDNLPSLQATVNLLKSTTIQKVRLFGAEPAV 68

Query: 67  IKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGNEVISSM 126
           IKA ANTG+ IVIG  NGDIP LASNPN A+Q++ +NV+ +YPASNI+ ITVGNEV++S 
Sbjct: 69  IKAFANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAITVGNEVLTSG 128

Query: 127 DQGLVSQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVLEQMMKA 186
           DQ L+SQLLPAMQNVQNALN+A+LGGKVKVSTVH+MAVLSQS PPSSG FNP L   MKA
Sbjct: 129 DQKLISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLSQSYPPSSGVFNPGLGDTMKA 188

Query: 187 VVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNMFDAQLD 246
           +++F+  N +PF I+PYP+FAY++ P  +TLAFCLFQPN+G+VDSG G KY NMFDAQ+D
Sbjct: 189 LLQFQSANDAPFMISPYPYFAYKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMFDAQVD 248

Query: 247 AVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSMVGTPLM 306
           AV SALNA+G FKD+EI+VAETGW + GDSNEVG +++NA+AY GNLI HL+S VGTPLM
Sbjct: 249 AVHSALNAMG-FKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVGTPLM 308

Query: 307 PGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMAYDVGLSKNSQTPTTPTTPSPKPT 366
           PGKS+DTY+F+LYDE+ K G +SE+ FGLF+PD +  YDVGL KN+Q PTTP TP+P P 
Sbjct: 309 PGKSIDTYLFSLYDEDKKTGASSEKYFGLFKPDGSTTYDVGLLKNTQNPTTPATPTPTPK 368

Query: 367 AL-AWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAYAMNLYYQS 426
           A  +WC+PK G SD QL  N++YACG GIDCG IQPGGACFEPNTVK+HAAY MNLYYQS
Sbjct: 369 AAGSWCVPKPGVSDDQLTGNINYACGQGIDCGPIQPGGACFEPNTVKAHAAYVMNLYYQS 428

Query: 427 LGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 459
            G+N W+CDFSQTATLT+TNPSY  C++P GS+
Sbjct: 429 AGRNSWNCDFSQTATLTNTNPSYGACNFPSGSN 460

BLAST of Cp4.1LG16g02410 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.7e-100
Identity = 193/441 (43.76%), Postives = 274/441 (62.13%), Query Frame = 0

Query: 28  IGVNYGQVANNLPPPVSTAALLRS-TTIGKVRLYGADPQIIKALANTGIGIVIGVANGDI 87
           IGVNYG  A+NLP P S A  L + TTI +V+L+ A+P  I A A T I + + + N  +
Sbjct: 26  IGVNYGANADNLPSPTSVATFLATKTTIDRVKLFDANPTFISAFAGTPISLAVSLPNSAL 85

Query: 88  PALASNP---NSAAQWINTNVVPYYPASNIVLITVGNEVISSMDQGLVSQLLPAMQNVQN 147
           PALA      ++A  WI  N+ PY PA+N+ L+  GNE++ S D  L+  LLPAM+ +  
Sbjct: 86  PALADKATGLDAARSWIRANLSPYVPATNVTLLLAGNEILLSTDTNLILSLLPAMRRLAQ 145

Query: 148 ALNSANLGGKVKVSTVHSMAVLSQSDP-PSSGRFNPVLE-QMMKAVVEFEKENASPFAIN 207
           AL +  L G V+V+T H + +L+ SD  PS+  F      ++  A+++F ++  SPF +N
Sbjct: 146 ALKAEGLTG-VRVTTPHYLGILAPSDGIPSNASFRAGYNTKLFPAMLQFHRDTGSPFMVN 205

Query: 208 PYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNMFDAQLDAVRSALNALGDFKDV 267
           PYP+F+Y    R ETL + LF+PNSG  D  T + Y +M DAQ+DA+ +A+  LG + DV
Sbjct: 206 PYPYFSY----RPETLNYALFRPNSGIYDPATKLNYTSMLDAQMDAIYTAMKKLG-YGDV 265

Query: 268 EILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSMVGTPLMPGKSVDTYIFALYDE 327
           +I V E GW  + +  ++G  ++ AR +N  +I    S  GTPLMP ++ +TY+F+L+DE
Sbjct: 266 DIAVGEAGWPTQAEPGQIGVGVQEARDFNEGMIRVCSSGKGTPLMPNRTFETYLFSLFDE 325

Query: 328 NLKPGPTSERSFGLFRPDLTMAYDVGLSKN--SQTPTTPTTPSPKPTAL-------AWCM 387
           N KPGP +ER FGLF PD T  YD+GL ++  S  PT    PSP P+          WC+
Sbjct: 326 NQKPGPIAERHFGLFNPDFTPVYDLGLLRDGASVAPTPSPNPSPNPSPKPAPSGGGKWCV 385

Query: 388 PKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAYAMNLYYQSLGKNPWD 447
            K GA+   LQ N++YACG  +DC  IQ GGACF PN++++HA+Y MN YYQ+ G     
Sbjct: 386 AKDGANGTDLQNNINYACGF-VDCKPIQSGGACFSPNSLQAHASYVMNAYYQANGHTDLA 445

Query: 448 CDFSQTATLTSTNPSYNGCSY 454
           CDF  T  +TS++PSY GC Y
Sbjct: 446 CDFKGTGIVTSSDPSYGGCKY 459

BLAST of Cp4.1LG16g02410 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 2.6e-96
Identity = 195/469 (41.58%), Postives = 277/469 (59.06%), Query Frame = 0

Query: 10  FVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLYGADPQIIKA 69
           FVS   F  F +A   S IG+ YG+ A+NLP P   + L++   I  VR+Y A+  ++KA
Sbjct: 12  FVSILAFLNFGMA---SKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKA 71

Query: 70  LANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGNEVISSMDQG 129
            ANTGI ++IGV N D+ A A   ++   W++ N++PYYP++ I  I+VG EV  + D  
Sbjct: 72  FANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNA 131

Query: 130 LVSQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVLEQMMKAVVE 189
               +LPAM+N+  AL  + L  K+K+S+ HS+A+LS+S PPSS  F+      +K ++E
Sbjct: 132 -TGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSAFLKPMLE 191

Query: 190 FEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNMFDAQLDAVR 249
           F  EN SPF I+ YP++AY+       L + LF+ +S  VD  TG+ Y NMFDAQLDA+ 
Sbjct: 192 FLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIY 251

Query: 250 SALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSMVGTPLMPGK 309
            AL A+  FK V+++V E+GW  +G   E   T ENA AYN NLI H+    GTP  PG+
Sbjct: 252 FALTAM-SFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGE 311

Query: 310 SVDTYIFALYDENLKPGPTSERSFGLFRPDLTMAYDVGLSKNSQTP------TTPTTPSP 369
            +D Y+F+L++EN KPG  SER++G+F  + T  Y +  +  + TP      TT T+PSP
Sbjct: 312 EIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSP 371

Query: 370 KPTAL------------------AWCMPKAGASDAQLQLNLDYACGHG-IDCGAIQPGGA 429
             + +                   WC+  + AS  +LQ  LD+ACG G +DC A+QP   
Sbjct: 372 SSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQP 431

Query: 430 CFEPNTVKSHAAYAMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSY 454
           CFEP+TV SHA+YA N YYQ  G +  DC F+  +     +PSY  C Y
Sbjct: 432 CFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNCLY 475

BLAST of Cp4.1LG16g02410 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 3.7e-87
Identity = 168/429 (39.16%), Postives = 258/429 (60.14%), Query Frame = 0

Query: 28  IGVNYGQVANNLPPPVSTAALLRSTTIGKVRLYGADPQIIKALANTGIGIVIGVANGDIP 87
           +GV YG+ A++LP P     L++   I  VR+Y  + Q++KA  NT I ++IGV N D+ 
Sbjct: 26  VGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAFGNTSIELMIGVPNSDLN 85

Query: 88  ALASNPNSAAQWINTNVVPYYPASNIVLITVGNEVISSMDQGLVSQLLPAMQNVQNALNS 147
           A + + ++   W+  +V+PYYP + I  ITVG E          S ++PAMQNV  AL  
Sbjct: 86  AFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHINASSFVVPAMQNVLTALRK 145

Query: 148 ANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVLEQMMKAVVEFEKENASPFAINPYPFFA 207
             L  ++KVST  S+ +LS+S PPS+G FN      ++ ++EF  EN SPF I+ YP++A
Sbjct: 146 VGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYAYFLRPMLEFLAENKSPFMIDLYPYYA 205

Query: 208 YQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNMFDAQLDAVRSALNALGDFKDVEILVAE 267
           Y+  P   +L + LF+ +S  +D  TG+ Y NMFDAQ+DA+  AL AL +F+ ++I+V E
Sbjct: 206 YRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDALYYALTAL-NFRTIKIMVTE 265

Query: 268 TGWAYRGDSNE-VGTTMENARAYNGNLIAHLRSMVGTPLMPGKSVDTYIFALYDENLKPG 327
           TGW  +G   E    + +NA  YN N+I H+ +  GTP  PG++++ YIF+L++EN K G
Sbjct: 266 TGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPGEAMNVYIFSLFNENRKAG 325

Query: 328 PTSERSFGLFRPDLTMAYDVGLS-KNSQTPTTPTTPSPKPTALAWCMPKAGASDAQLQLN 387
             SER++GLF PD T  Y +  + K++   +  +  +   ++ +WC+  + AS+  L+  
Sbjct: 326 LDSERNWGLFYPDQTSVYQLDFTGKSNGFHSNSSGTNSSGSSNSWCIASSKASERDLKGA 385

Query: 388 LDYACGHG-IDCGAIQPGGACFEPNTVKSHAAYAMNLYYQSLGKNPWDCDFSQTATLTST 447
           LD+ACG G +DC AIQP   CF+P+T+ SHA++  N Y+Q        C F       + 
Sbjct: 386 LDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNSYFQQNRATDVACSFGGAGVKVNK 445

Query: 448 NPSYNGCSY 454
           +PSY+ C Y
Sbjct: 446 DPSYDKCIY 453

BLAST of Cp4.1LG16g02410 vs. NCBI nr
Match: XP_023512642.1 (glucan endo-1,3-beta-D-glucosidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 912 bits (2356), Expect = 0.0
Identity = 458/458 (100.00%), Postives = 458/458 (100.00%), Query Frame = 0

Query: 1   MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60
           MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY
Sbjct: 1   MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60

Query: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120
           GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN
Sbjct: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120

Query: 121 EVISSMDQGLVSQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180
           EVISSMDQGLVSQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL
Sbjct: 121 EVISSMDQGLVSQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180

Query: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240
           EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM
Sbjct: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240

Query: 241 FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM 300
           FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM
Sbjct: 241 FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMAYDVGLSKNSQTPTTPTT 360
           VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMAYDVGLSKNSQTPTTPTT
Sbjct: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMAYDVGLSKNSQTPTTPTT 360

Query: 361 PSPKPTALAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAYAMN 420
           PSPKPTALAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAYAMN
Sbjct: 361 PSPKPTALAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAYAMN 420

Query: 421 LYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 458
           LYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS
Sbjct: 421 LYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 458

BLAST of Cp4.1LG16g02410 vs. NCBI nr
Match: KAG6571123.1 (Glucan endo-1,3-beta-glucosidase 7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010935.1 Glucan endo-1,3-beta-glucosidase 7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 902 bits (2332), Expect = 0.0
Identity = 455/461 (98.70%), Postives = 456/461 (98.92%), Query Frame = 0

Query: 1   MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60
           MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY
Sbjct: 1   MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60

Query: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120
           GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN
Sbjct: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120

Query: 121 EVISSMDQGLVSQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180
           EVISSMDQGL+SQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL
Sbjct: 121 EVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180

Query: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240
           EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM
Sbjct: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240

Query: 241 FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM 300
           FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM
Sbjct: 241 FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMAYDVGLSKNSQTPTTPTT 360
           VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTM YDVGLSKNSQTPTTPTT
Sbjct: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTT 360

Query: 361 P---SPKPTALAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY 420
           P   SPKPTA AWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY
Sbjct: 361 PATPSPKPTATAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY 420

Query: 421 AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 458
           AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS
Sbjct: 421 AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 461

BLAST of Cp4.1LG16g02410 vs. NCBI nr
Match: XP_022943626.1 (glucan endo-1,3-beta-D-glucosidase [Cucurbita moschata])

HSP 1 Score: 900 bits (2326), Expect = 0.0
Identity = 454/461 (98.48%), Postives = 455/461 (98.70%), Query Frame = 0

Query: 1   MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60
           MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY
Sbjct: 1   MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60

Query: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120
           GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN
Sbjct: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120

Query: 121 EVISSMDQGLVSQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180
           EVISSMDQGL+SQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL
Sbjct: 121 EVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180

Query: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240
           EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM
Sbjct: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240

Query: 241 FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM 300
           FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM
Sbjct: 241 FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMAYDVGLSKNSQTPTTPTT 360
           VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTM YDVGLSKNSQTPTTPTT
Sbjct: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTT 360

Query: 361 P---SPKPTALAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY 420
           P   SPKPTA AWCMPKAGASDAQLQ NLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY
Sbjct: 361 PATPSPKPTAAAWCMPKAGASDAQLQSNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY 420

Query: 421 AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 458
           AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS
Sbjct: 421 AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 461

BLAST of Cp4.1LG16g02410 vs. NCBI nr
Match: XP_022986522.1 (glucan endo-1,3-beta-D-glucosidase-like [Cucurbita maxima])

HSP 1 Score: 888 bits (2294), Expect = 0.0
Identity = 447/461 (96.96%), Postives = 449/461 (97.40%), Query Frame = 0

Query: 1   MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60
           MA+SSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY
Sbjct: 1   MASSSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60

Query: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120
           GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN
Sbjct: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120

Query: 121 EVISSMDQGLVSQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180
           EVISSMDQGL+SQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL
Sbjct: 121 EVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180

Query: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240
           EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM
Sbjct: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240

Query: 241 FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM 300
           FDAQLDAVRSALN LGDFKDVEILVAETGWAY GDSNEVGTTMENARAYNGNLIAHLRSM
Sbjct: 241 FDAQLDAVRSALNVLGDFKDVEILVAETGWAYHGDSNEVGTTMENARAYNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMAYDVGLSKNSQTPTTPTT 360
           VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTM YDVGLSKNSQTPTTPTT
Sbjct: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTT 360

Query: 361 PS---PKPTALAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY 420
           PS   PKPT   WCMPKAG SDAQLQ NLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY
Sbjct: 361 PSTPSPKPTVAVWCMPKAGVSDAQLQSNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY 420

Query: 421 AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 458
           AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSY GGSS
Sbjct: 421 AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYTGGSS 461

BLAST of Cp4.1LG16g02410 vs. NCBI nr
Match: XP_038900992.1 (glucan endo-1,3-beta-D-glucosidase-like [Benincasa hispida])

HSP 1 Score: 819 bits (2116), Expect = 9.34e-298
Identity = 410/456 (89.91%), Postives = 426/456 (93.42%), Query Frame = 0

Query: 5   SFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLYGADP 64
           SF L F+SF LFAA YLADSQSFIGVNYGQVA+NLPPP STA LLRST+I K+RLYGADP
Sbjct: 3   SFLLPFLSFFLFAAVYLADSQSFIGVNYGQVADNLPPPSSTANLLRSTSIAKIRLYGADP 62

Query: 65  QIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGNEVIS 124
            IIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNV PYYPASNIVL+TVGNEV+S
Sbjct: 63  LIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVNPYYPASNIVLVTVGNEVMS 122

Query: 125 SMDQGLVSQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVLEQMM 184
           SMDQ L+SQL+PAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPV EQ M
Sbjct: 123 SMDQNLISQLVPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVFEQTM 182

Query: 185 KAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNMFDAQ 244
           KAVVEF KE  SPFAINPYPFFAYQSDPR ETLAFCLFQPNSGRVDSG+GIKYMNMFDAQ
Sbjct: 183 KAVVEFLKEKKSPFAINPYPFFAYQSDPRPETLAFCLFQPNSGRVDSGSGIKYMNMFDAQ 242

Query: 245 LDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSMVGTP 304
           LDAVRSALNALGDFKDVEI+VAETGWAYRGDSNEVG ++ENARAYNGNLIAHLRSMVGTP
Sbjct: 243 LDAVRSALNALGDFKDVEIMVAETGWAYRGDSNEVGPSVENARAYNGNLIAHLRSMVGTP 302

Query: 305 LMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMAYDVGLSKNSQTPTTPTTP--- 364
           LMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTM YDVGLSKNSQTPTTPTTP   
Sbjct: 303 LMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTTPATP 362

Query: 365 SPKPTALAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAYAMNL 424
           SP+PTA AWC+PKAG  D QLQ NLDYACGHGIDCG IQPGGACFEPNTV SHAAYAMNL
Sbjct: 363 SPEPTAPAWCLPKAGIPDDQLQSNLDYACGHGIDCGPIQPGGACFEPNTVHSHAAYAMNL 422

Query: 425 YYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGS 457
           YYQSLGKNPW+CDFSQTA LTSTNPSYN C+YPGGS
Sbjct: 423 YYQSLGKNPWNCDFSQTAILTSTNPSYNACTYPGGS 458

BLAST of Cp4.1LG16g02410 vs. ExPASy TrEMBL
Match: A0A6J1FS76 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111448341 PE=3 SV=1)

HSP 1 Score: 900 bits (2326), Expect = 0.0
Identity = 454/461 (98.48%), Postives = 455/461 (98.70%), Query Frame = 0

Query: 1   MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60
           MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY
Sbjct: 1   MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60

Query: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120
           GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN
Sbjct: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120

Query: 121 EVISSMDQGLVSQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180
           EVISSMDQGL+SQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL
Sbjct: 121 EVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180

Query: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240
           EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM
Sbjct: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240

Query: 241 FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM 300
           FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM
Sbjct: 241 FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMAYDVGLSKNSQTPTTPTT 360
           VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTM YDVGLSKNSQTPTTPTT
Sbjct: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTT 360

Query: 361 P---SPKPTALAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY 420
           P   SPKPTA AWCMPKAGASDAQLQ NLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY
Sbjct: 361 PATPSPKPTAAAWCMPKAGASDAQLQSNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY 420

Query: 421 AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 458
           AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS
Sbjct: 421 AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 461

BLAST of Cp4.1LG16g02410 vs. ExPASy TrEMBL
Match: A0A6J1J7S0 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484236 PE=3 SV=1)

HSP 1 Score: 888 bits (2294), Expect = 0.0
Identity = 447/461 (96.96%), Postives = 449/461 (97.40%), Query Frame = 0

Query: 1   MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60
           MA+SSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY
Sbjct: 1   MASSSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60

Query: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120
           GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN
Sbjct: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120

Query: 121 EVISSMDQGLVSQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180
           EVISSMDQGL+SQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL
Sbjct: 121 EVISSMDQGLISQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVL 180

Query: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240
           EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM
Sbjct: 181 EQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNM 240

Query: 241 FDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSM 300
           FDAQLDAVRSALN LGDFKDVEILVAETGWAY GDSNEVGTTMENARAYNGNLIAHLRSM
Sbjct: 241 FDAQLDAVRSALNVLGDFKDVEILVAETGWAYHGDSNEVGTTMENARAYNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMAYDVGLSKNSQTPTTPTT 360
           VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTM YDVGLSKNSQTPTTPTT
Sbjct: 301 VGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMTYDVGLSKNSQTPTTPTT 360

Query: 361 PS---PKPTALAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY 420
           PS   PKPT   WCMPKAG SDAQLQ NLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY
Sbjct: 361 PSTPSPKPTVAVWCMPKAGVSDAQLQSNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAY 420

Query: 421 AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGSS 458
           AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSY GGSS
Sbjct: 421 AMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYTGGSS 461

BLAST of Cp4.1LG16g02410 vs. ExPASy TrEMBL
Match: A0A1S3C673 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103497492 PE=3 SV=1)

HSP 1 Score: 792 bits (2045), Expect = 3.57e-287
Identity = 395/461 (85.68%), Postives = 422/461 (91.54%), Query Frame = 0

Query: 5   SFSLTFVSFSLFAA-----FYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRL 64
           SFSL F S  LFAA     FY+ADSQSFIGVNYGQ+++NLPPP STA LLRST+I K+RL
Sbjct: 3   SFSLPFFSLFLFAAASSTVFYIADSQSFIGVNYGQLSDNLPPPSSTANLLRSTSISKIRL 62

Query: 65  YGADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVG 124
           Y ADP IIK+LAN+G+GIVIG+ANGDIPALASNPNSAAQWINTN+ PYYPASNI+LITVG
Sbjct: 63  YNADPLIIKSLANSGLGIVIGIANGDIPALASNPNSAAQWINTNLNPYYPASNILLITVG 122

Query: 125 NEVISSMDQGLVSQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPV 184
           NEV+SSMDQ L+SQL+PAM+NVQNA+N+A+LGGKVKVSTVHSMAVLSQSDPPSSGR NP+
Sbjct: 123 NEVMSSMDQNLISQLVPAMRNVQNAVNAASLGGKVKVSTVHSMAVLSQSDPPSSGRINPM 182

Query: 185 LEQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMN 244
            E  MKAVVEF KEN SPFAINPYPFFAYQSDPR ETLAFCLFQPNSGRVDSG GIKYMN
Sbjct: 183 FETTMKAVVEFLKENESPFAINPYPFFAYQSDPRDETLAFCLFQPNSGRVDSGNGIKYMN 242

Query: 245 MFDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRS 304
           MFDAQLDAVRS LNALGDFKDVEI+VAETGWAYRGDSNEVGT++ENARAYNGNLIAHLRS
Sbjct: 243 MFDAQLDAVRSGLNALGDFKDVEIMVAETGWAYRGDSNEVGTSVENARAYNGNLIAHLRS 302

Query: 305 MVGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMAYDVGLSKNSQTPTTPT 364
           MVGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLF P+LTM YDVGLSKNSQTPTTPT
Sbjct: 303 MVGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFYPNLTMTYDVGLSKNSQTPTTPT 362

Query: 365 TP---SPKPTALAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAA 424
           TP   SPKPTA  WC+PKA   D QLQ NLDYACGHGIDCG IQPGGACFEPNTVKSHAA
Sbjct: 363 TPTTPSPKPTAPVWCLPKADIPDDQLQSNLDYACGHGIDCGPIQPGGACFEPNTVKSHAA 422

Query: 425 YAMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGS 457
           YAMNLYYQSLGKNPW+CDFSQTATLTSTNPSYN C+YPGGS
Sbjct: 423 YAMNLYYQSLGKNPWNCDFSQTATLTSTNPSYNACTYPGGS 463

BLAST of Cp4.1LG16g02410 vs. ExPASy TrEMBL
Match: A0A0A0LL52 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G246520 PE=3 SV=1)

HSP 1 Score: 783 bits (2023), Expect = 8.00e-284
Identity = 390/461 (84.60%), Postives = 420/461 (91.11%), Query Frame = 0

Query: 5   SFSLTFVSFSLFAA-----FYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRL 64
           SFSL F SF LFAA     FY+ADSQSFIGVNYGQ+++NLPPP +TA LLRST+I K+RL
Sbjct: 3   SFSLPFFSFFLFAAASSAVFYIADSQSFIGVNYGQLSDNLPPPSATANLLRSTSISKIRL 62

Query: 65  YGADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVG 124
           Y ADP IIK+LAN+G+GIVIG+ANGDIPALASNPNSAAQWINTN+ PYYPASNI+LITVG
Sbjct: 63  YNADPLIIKSLANSGLGIVIGIANGDIPALASNPNSAAQWINTNLNPYYPASNILLITVG 122

Query: 125 NEVISSMDQGLVSQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPV 184
           NEV+SSMDQ L+SQL+PAM+NVQNA+N+ANLGGKVKVSTVHSMAVLSQSDPPSSGR NP+
Sbjct: 123 NEVMSSMDQNLISQLVPAMRNVQNAVNAANLGGKVKVSTVHSMAVLSQSDPPSSGRINPM 182

Query: 185 LEQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMN 244
            E  MK VVEF KEN SPFAINPYPFFAYQSDPR ETLAFCLFQPNSGRVDSG GIKYMN
Sbjct: 183 FEGTMKEVVEFLKENESPFAINPYPFFAYQSDPRDETLAFCLFQPNSGRVDSGNGIKYMN 242

Query: 245 MFDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRS 304
           MFDAQLDAVRSALNALG FKDVEI+VAETGWAYRGDSNEVGT++ENARAYNGNLIAHLRS
Sbjct: 243 MFDAQLDAVRSALNALGGFKDVEIMVAETGWAYRGDSNEVGTSVENARAYNGNLIAHLRS 302

Query: 305 MVGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMAYDVGLSKNSQTPTTPT 364
           MVGTPLMPGKSVDTYIFALYDENLKPGPTSER+FGLF P+LTM YDVGLSKNSQTPTTPT
Sbjct: 303 MVGTPLMPGKSVDTYIFALYDENLKPGPTSERAFGLFYPNLTMTYDVGLSKNSQTPTTPT 362

Query: 365 TP---SPKPTALAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAA 424
           TP   S KPTA  WC+PKA   D QLQ NLDYACGHGIDCG IQPGGACFEPNT++SHAA
Sbjct: 363 TPTTPSSKPTAPVWCLPKADIPDDQLQSNLDYACGHGIDCGPIQPGGACFEPNTIQSHAA 422

Query: 425 YAMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGGS 457
           YAMNLYYQSLGKNPW+CDFSQTATLTS NPSYN C+YPGGS
Sbjct: 423 YAMNLYYQSLGKNPWNCDFSQTATLTSANPSYNACTYPGGS 463

BLAST of Cp4.1LG16g02410 vs. ExPASy TrEMBL
Match: A0A5D3CQ55 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G001950 PE=3 SV=1)

HSP 1 Score: 781 bits (2017), Expect = 2.09e-282
Identity = 385/448 (85.94%), Postives = 413/448 (92.19%), Query Frame = 0

Query: 13  FSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLYGADPQIIKALAN 72
           F+ F   + ADSQSFIGVNYGQ+++NLPPP STA LLRST+I K+RLY ADP IIK+LAN
Sbjct: 47  FTPFFFLFFADSQSFIGVNYGQLSDNLPPPSSTANLLRSTSISKIRLYNADPLIIKSLAN 106

Query: 73  TGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGNEVISSMDQGLVS 132
           +G+GIVIG+ANGDIPALASNPNSAAQWINTN+ PYYPASNI+LITVGNEV+SSMDQ L+S
Sbjct: 107 SGLGIVIGIANGDIPALASNPNSAAQWINTNLNPYYPASNILLITVGNEVMSSMDQNLIS 166

Query: 133 QLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVLEQMMKAVVEFEK 192
           QL+PAM+NVQNA+N+A+LGGKVKVSTVHSMAVLSQSDPPSSGR NP+ E  MKAVVEF K
Sbjct: 167 QLVPAMRNVQNAVNAASLGGKVKVSTVHSMAVLSQSDPPSSGRINPMFETTMKAVVEFLK 226

Query: 193 ENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNMFDAQLDAVRSAL 252
           EN SPFAINPYPFFAYQSDPR ETLAFCLFQPNSGRVDSG GIKYMNMFDAQLDAVRS L
Sbjct: 227 ENESPFAINPYPFFAYQSDPRDETLAFCLFQPNSGRVDSGNGIKYMNMFDAQLDAVRSGL 286

Query: 253 NALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSMVGTPLMPGKSVD 312
           NALGDFKDVEI+VAETGWAYRGDSNEVGT++ENARAYNGNLIAHLRSMVGTPLMPGKSVD
Sbjct: 287 NALGDFKDVEIMVAETGWAYRGDSNEVGTSVENARAYNGNLIAHLRSMVGTPLMPGKSVD 346

Query: 313 TYIFALYDENLKPGPTSERSFGLFRPDLTMAYDVGLSKNSQTPTTPTTP---SPKPTALA 372
           TYIFALYDENLKPGPTSERSFGLF P+LTM YDVGLSKNSQTPTTPTTP   SPKPTA  
Sbjct: 347 TYIFALYDENLKPGPTSERSFGLFYPNLTMTYDVGLSKNSQTPTTPTTPTTPSPKPTAPV 406

Query: 373 WCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAYAMNLYYQSLGKN 432
           WC+PKA   D QLQ NLDYACGHGIDCG IQPGGACFEPNTVKSHAAYAMNLYYQSLGKN
Sbjct: 407 WCLPKADIPDDQLQSNLDYACGHGIDCGPIQPGGACFEPNTVKSHAAYAMNLYYQSLGKN 466

Query: 433 PWDCDFSQTATLTSTNPSYNGCSYPGGS 457
           PW+CDFSQTATLTSTNPSYN C+YPGGS
Sbjct: 467 PWNCDFSQTATLTSTNPSYNACTYPGGS 494

BLAST of Cp4.1LG16g02410 vs. TAIR 10
Match: AT4G34480.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 634.8 bits (1636), Expect = 5.4e-182
Identity = 314/454 (69.16%), Postives = 376/454 (82.82%), Query Frame = 0

Query: 3   ASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLYGA 62
           A S S+ F+   L + F  + ++ FIGVNYGQVA+NLPPP  T  LL+ST+I KVRLYGA
Sbjct: 2   ALSISIYFLLIFL-SHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGA 61

Query: 63  DPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGNEV 122
           DP IIKALA TG+GIVIG ANGD+P+LAS+PN+A QWIN+NV+P+YPAS I+LITVGNE+
Sbjct: 62  DPAIIKALAGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEI 121

Query: 123 ISSMDQGLVSQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVLEQ 182
           + S D  LV+QLLPAMQNVQ AL + +LGGK+KVSTV+SM VL  SDPPSSG F    + 
Sbjct: 122 LMSNDPNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQT 181

Query: 183 MMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNMFD 242
            +K +++F  +  SPFAINPYPFFAYQSDPR ETLAFCLF+PN+GRVDS TGIKY NMFD
Sbjct: 182 GLKGILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFD 241

Query: 243 AQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSMVG 302
           AQ+DAV SAL ++G F+ VEI+VAETGWA RGD+NEVG +++NA+AYNGNLIAHLRSMVG
Sbjct: 242 AQVDAVHSALKSMG-FEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVG 301

Query: 303 TPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMAYDVGLSKNSQTPTTPTTPS 362
           TPLMPGK VDTYIFALYDENLKPGP+SER+FGLF+ DL+M YDVGL+K+S   ++  TPS
Sbjct: 302 TPLMPGKPVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLAKSS---SSSQTPS 361

Query: 363 PKPTALAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAYAMNLY 422
            K T+  WC+PK GA++ +LQ +LD+ACGHGIDCGAIQPGGACFEPN V SHAAYAMN+Y
Sbjct: 362 GKVTSSGWCVPKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMY 421

Query: 423 YQSLGKNPWDCDFSQTATLTSTNPSYNGCSYPGG 457
           +Q   K P DCDFS+TAT+TS NPSYN C YPGG
Sbjct: 422 FQKSPKQPTDCDFSKTATVTSQNPSYNNCVYPGG 450

BLAST of Cp4.1LG16g02410 vs. TAIR 10
Match: AT2G16230.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 608.6 bits (1568), Expect = 4.1e-174
Identity = 297/449 (66.15%), Postives = 362/449 (80.62%), Query Frame = 0

Query: 6   FSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLYGADPQ 65
           F L F+ FS+      +++QSFIGVNYG +++NLPPP  TA LL+ST+I KVRLY AD  
Sbjct: 7   FLLLFILFSISP----SNAQSFIGVNYGLLSDNLPPPSQTAKLLQSTSIQKVRLYNADSS 66

Query: 66  IIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGNEVISS 125
           II +L  TGIGIVIGVANGD+P++AS+ N A+QWIN+NV+P+YPASNI+LI VGNEV+ S
Sbjct: 67  IITSLVGTGIGIVIGVANGDLPSIASDLNIASQWINSNVLPFYPASNIILINVGNEVLLS 126

Query: 126 MDQGLVSQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNPVLEQMMK 185
            D  LV+QLLPAMQNVQ AL + +LGGK+KVSTVH+M VL  S+PPS+G F P  +  +K
Sbjct: 127 NDLNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVHAMTVLGNSEPPSAGSFAPSYQAGLK 186

Query: 186 AVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMNMFDAQL 245
            +++F  +  SPFAINPYPFFAYQSDPR ETLAFCLFQPN GRVDS TGIKYMNMFDAQ+
Sbjct: 187 GILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFQPNPGRVDSNTGIKYMNMFDAQV 246

Query: 246 DAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRSMVGTPL 305
           DAV SAL ++G F+ VE+LVAETGW   GDSNEVG ++ENA+AYNGNLIAHLRSMVGTPL
Sbjct: 247 DAVHSALKSIG-FEKVEVLVAETGWPSTGDSNEVGPSVENAKAYNGNLIAHLRSMVGTPL 306

Query: 306 MPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMAYDVGLSKNSQTPTTPTTPSPKP 365
           MPGKS+DTYIFAL+DENLKPGP+ E+SFGLF+PDL+MAYD+GL+K + + T+ +    K 
Sbjct: 307 MPGKSIDTYIFALFDENLKPGPSFEQSFGLFKPDLSMAYDIGLTKTTSSQTSQSPQLGKV 366

Query: 366 TALAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKSHAAYAMNLYYQS 425
           T++ WC+PK  A+  QLQ +LD+ CG GIDCG I PGG CFEPN V SH AYAMNLY+Q 
Sbjct: 367 TSMGWCVPKEDATQEQLQDSLDWVCGQGIDCGPIMPGGVCFEPNNVASHTAYAMNLYFQK 426

Query: 426 LGKNPWDCDFSQTATLTSTNPSYNGCSYP 455
             +NP DCDFS+TA +TS NPSY+ C YP
Sbjct: 427 SPENPTDCDFSKTARITSENPSYSSCVYP 450

BLAST of Cp4.1LG16g02410 vs. TAIR 10
Match: AT5G42720.1 (Glycosyl hydrolase family 17 protein )

HSP 1 Score: 465.7 bits (1197), Expect = 4.3e-131
Identity = 240/374 (64.17%), Postives = 287/374 (76.74%), Query Frame = 0

Query: 1   MAASSFSLTFVSFSLFAAFYLADSQSFIGVNYGQVANNLPPPVSTAALLRSTTIGKVRLY 60
           M AS +SL  + FS     +L+ SQ F+GVNYG  A+NLPPP ++A LL+STT  KVRLY
Sbjct: 1   MRASVYSLILLFFS--CLLHLSKSQPFLGVNYGLTADNLPPPSASAKLLQSTTFQKVRLY 60

Query: 61  GADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITVGN 120
           G+DP +IKALANTGI IVIG +NGD+P LAS+P+ A  W+ TNVVPYYPAS IVLI VGN
Sbjct: 61  GSDPAVIKALANTGIEIVIGASNGDVPGLASDPSFARSWVETNVVPYYPASKIVLIAVGN 120

Query: 121 EVISSMDQGLVSQLLPAMQNVQNALNSANL-GGKVKVSTVHSMAVLSQSDPPSSGRFNPV 180
           E+ S  D  L+SQLLPAM+NVQ AL +A+L GGK+KVSTVH M+VL+ SDPPS+  F P 
Sbjct: 121 EITSFGDNSLMSQLLPAMKNVQTALEAASLGGGKIKVSTVHIMSVLAGSDPPSTAVFKPE 180

Query: 181 LEQMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKYMN 240
              ++K ++EF  E  SPFA+NPYPFFAYQ D R ETLA+CLFQ N GRVD  + +KYMN
Sbjct: 181 HADILKGLLEFNSETGSPFAVNPYPFFAYQDDRRPETLAYCLFQANPGRVDPNSNLKYMN 240

Query: 241 MFDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHLRS 300
           MFDAQ+DAV SALN++G FKDVEI+VAETGW Y+GD  E G T+ENARAYN NLIAHL+S
Sbjct: 241 MFDAQVDAVYSALNSMG-FKDVEIMVAETGWPYKGDPEEAGATVENARAYNKNLIAHLKS 300

Query: 301 MVGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMAYDVGLSK------NSQ 360
             GTPLMPG+ +DTY+FALYDENLKPG  SER+FGLFRPDLTM YD+GL+K       S 
Sbjct: 301 GSGTPLMPGRVIDTYLFALYDENLKPGKGSERAFGLFRPDLTMTYDIGLTKTTNYNQTSM 360

Query: 361 TPTTPTTPSPKPTA 368
            P +PT P   P A
Sbjct: 361 APLSPTRPRLPPAA 371

BLAST of Cp4.1LG16g02410 vs. TAIR 10
Match: AT5G24318.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 424.5 bits (1090), Expect = 1.1e-118
Identity = 219/460 (47.61%), Postives = 304/460 (66.09%), Query Frame = 0

Query: 5   SFSLTFVSFSLFA-----AFYLADSQSFIGVNYGQVANNLPPPVSTAA-LLRSTTIGKVR 64
           ++ L F S S+F            S++ IGVNYG +ANNLPPP   A  LL ST I ++R
Sbjct: 2   NYVLPFFSLSMFTIVGVLLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIR 61

Query: 65  LYGADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITV 124
           L+  DPQI++A ANTGI + + V N  IP L +N +SA QWI+ ++ P++P++NI+ I V
Sbjct: 62  LFDTDPQILQAFANTGIAVTVTVPNDQIPHL-TNLSSAKQWISDHIQPHFPSTNIIRILV 121

Query: 125 GNEVISSMDQGLVSQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNP 184
           GNEVIS+ D  L+  L+PAMQ++  AL SA+L  ++++ST HS+  L+ S PPSS +F  
Sbjct: 122 GNEVISTADHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRR 181

Query: 185 VLE-QMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKY 244
             + Q++K ++ F +  +SPF +NPYPFF Y      ETL F LF+PN G  D  T + Y
Sbjct: 182 GYDAQVLKPLLSFLRSTSSPFVVNPYPFFGYS----IETLDFALFRPNPGLFDQHTKLLY 241

Query: 245 MNMFDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHL 304
            NM DAQLD+V SA++ LG F DVEI++ E GW   GD +++G  ++ A  +N NLIA +
Sbjct: 242 TNMLDAQLDSVYSAMDKLG-FSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARV 301

Query: 305 RSMVGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMAYDVGL----SKNSQ 364
            S  GTPLMP ++ +TYIFAL++ENLK GPTSER+FG+FR DLT  YD+G+     ++S 
Sbjct: 302 DSGTGTPLMPNRTFETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSD 361

Query: 365 TPTTPTTPSPKPTALAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKS 424
               P +P    ++  WC+ KAGA    LQ N+DY CG G+DC  I  GG C+ PNTVK+
Sbjct: 362 PVYNPRSPVRGSSSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKA 421

Query: 425 HAAYAMNLYYQSLGKNPWDCDFSQTATLTSTNPSYNGCSY 454
           H+ YAMNLYYQ++ K+ +DCDF  T  +T+ +PSY  C Y
Sbjct: 422 HSKYAMNLYYQTMEKHEFDCDFDNTGEITTIDPSYGNCEY 455

BLAST of Cp4.1LG16g02410 vs. TAIR 10
Match: AT5G24318.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 417.9 bits (1073), Expect = 1.0e-116
Identity = 217/456 (47.59%), Postives = 302/456 (66.23%), Query Frame = 0

Query: 5   SFSLTFVSFSLFA-----AFYLADSQSFIGVNYGQVANNLPPPVSTAA-LLRSTTIGKVR 64
           ++ L F S S+F            S++ IGVNYG +ANNLPPP   A  LL ST I ++R
Sbjct: 2   NYVLPFFSLSMFTIVGVLLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIR 61

Query: 65  LYGADPQIIKALANTGIGIVIGVANGDIPALASNPNSAAQWINTNVVPYYPASNIVLITV 124
           L+  DPQI++A ANTGI + + V N  IP L +N +SA QWI+ ++ P++P++NI+ I V
Sbjct: 62  LFDTDPQILQAFANTGIAVTVTVPNDQIPHL-TNLSSAKQWISDHIQPHFPSTNIIRILV 121

Query: 125 GNEVISSMDQGLVSQLLPAMQNVQNALNSANLGGKVKVSTVHSMAVLSQSDPPSSGRFNP 184
           GNEVIS+ D  L+  L+PAMQ++  AL SA+L  ++++ST HS+  L+ S PPSS +F  
Sbjct: 122 GNEVISTADHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRR 181

Query: 185 VLE-QMMKAVVEFEKENASPFAINPYPFFAYQSDPRAETLAFCLFQPNSGRVDSGTGIKY 244
             + Q++K ++ F +  +SPF +NPYPFF Y      ETL F LF+PN G  D  T + Y
Sbjct: 182 GYDAQVLKPLLSFLRSTSSPFVVNPYPFFGYS----IETLDFALFRPNPGLFDQHTKLLY 241

Query: 245 MNMFDAQLDAVRSALNALGDFKDVEILVAETGWAYRGDSNEVGTTMENARAYNGNLIAHL 304
            NM DAQLD+V SA++ LG F DVEI++ E GW   GD +++G  ++ A  +N NLIA +
Sbjct: 242 TNMLDAQLDSVYSAMDKLG-FSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARV 301

Query: 305 RSMVGTPLMPGKSVDTYIFALYDENLKPGPTSERSFGLFRPDLTMAYDVGL----SKNSQ 364
            S  GTPLMP ++ +TYIFAL++ENLK GPTSER+FG+FR DLT  YD+G+     ++S 
Sbjct: 302 DSGTGTPLMPNRTFETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSD 361

Query: 365 TPTTPTTPSPKPTALAWCMPKAGASDAQLQLNLDYACGHGIDCGAIQPGGACFEPNTVKS 424
               P +P    ++  WC+ KAGA    LQ N+DY CG G+DC  I  GG C+ PNTVK+
Sbjct: 362 PVYNPRSPVRGSSSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKA 421

Query: 425 HAAYAMNLYYQSLGKNPWDCDFSQTATLTSTNPSYN 450
           H+ YAMNLYYQ++ K+ +DCDF  T  +T+ +PS N
Sbjct: 422 HSKYAMNLYYQTMEKHEFDCDFDNTGEITTIDPSMN 451

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M0697.5e-18169.16Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Q94G861.3e-18068.21Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1[more]
P524091.7e-10043.76Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1[more]
Q8VYE52.6e-9641.58Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Q9FJU93.7e-8739.16Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590... [more]
Match NameE-valueIdentityDescription
XP_023512642.10.0100.00glucan endo-1,3-beta-D-glucosidase [Cucurbita pepo subsp. pepo][more]
KAG6571123.10.098.70Glucan endo-1,3-beta-glucosidase 7, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022943626.10.098.48glucan endo-1,3-beta-D-glucosidase [Cucurbita moschata][more]
XP_022986522.10.096.96glucan endo-1,3-beta-D-glucosidase-like [Cucurbita maxima][more]
XP_038900992.19.34e-29889.91glucan endo-1,3-beta-D-glucosidase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1FS760.098.48(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111448341 P... [more]
A0A6J1J7S00.096.96(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111484236 PE=... [more]
A0A1S3C6733.57e-28785.68(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103497492 PE=3 SV... [more]
A0A0A0LL528.00e-28484.60(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G246520 PE=3... [more]
A0A5D3CQ552.09e-28285.94(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
Match NameE-valueIdentityDescription
AT4G34480.15.4e-18269.16O-Glycosyl hydrolases family 17 protein [more]
AT2G16230.14.1e-17466.15O-Glycosyl hydrolases family 17 protein [more]
AT5G42720.14.3e-13164.17Glycosyl hydrolase family 17 protein [more]
AT5G24318.11.1e-11847.61O-Glycosyl hydrolases family 17 protein [more]
AT5G24318.21.0e-11647.59O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 369..453
e-value: 9.7E-46
score: 167.9
IPR012946X8 domainPFAMPF07983X8coord: 370..440
e-value: 2.3E-21
score: 76.2
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 28..346
e-value: 5.3E-82
score: 275.9
NoneNo IPR availableGENE3D1.20.58.1040coord: 366..453
e-value: 3.7E-10
score: 42.0
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 27..355
e-value: 1.0E-123
score: 414.4
NoneNo IPR availablePANTHERPTHR32227:SF227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE 7coord: 5..450
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 5..450
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 28..347

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG16g02410.1Cp4.1LG16g02410.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds