Cp4.1LG16g02280 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG16g02280
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description4-hydroxy-3-methylbut-2-enyl diphosphate reductase
LocationCp4.1LG16: 4379137 .. 4383178 (-)
RNA-Seq ExpressionCp4.1LG16g02280
SyntenyCp4.1LG16g02280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAGGGAAATAAAGTTTAAAGTTCTTCCAAAAAAGTTCGATGTTTTTCACTCGTAGTCGTTCCATGCTTTCAACCACCCACCATCTCTGCTAATCGGAGGAGGTAATCTGTAAAGCAGGATCAAGATTCGGTATACCTGTTCATTGATTTCAGTATTTTAATGAGTAGAACTTTTCCGGATTTGGACTTTGAATTTGAAACGTAATTTACGAAGCACCAGCAATGCTGTGCGTTCATATTTCTACGTCAACGTCCATTCTTCGTTGTTGTTATACCACCGTTTTCCATGGCGATTTCTCTGCAGATCCCCCGTCTTCCCCGTCGTGTAGACTCTGTTTCATCGAAGAGTTTTTCCGGCGCCGGAGCTCTCCGGTTCCGGAAACCCCTTTTGGTTCGGTGCACTGCTGATTCTTCGTCGGTCAATCCCGATTTCGATGCGAAGCTGTTCTGGAAGAACTTTTCCAGAGGTGAAAACTATAATCGGAAAGGGTTTGGGTATAAGGAGGAGGCGTTGAAGCTCATGAGTAGCGAGTTCACAAGTGAGTTTTTGTTGTATTTGAATGATGATTTTAGTTTGTTGAATCTTCGTTTTGTTGGTGCCAATTTTTTTGTATTGAATGGCTTAGGTGAAGTTGTGAATACTCTGAATGAAAATGGGAATCGGTTCACTTGGGGGAATGTTACTGTCAAATTGGCTGGAGCTTATGGCTTTTGTTGGGGAGTGGAACGAGCTGTGCAGATTGCTTATGAAGCGAGGAAACAGTTTCCGAGTGAAACAATATGGATTACCAATGAGATTATTCATAACCCAACTGTTAACAAGGTCCTTTTGATGCTTTAGCTTTTCTTGCCTTTACTGTTTCATTGGTTACACAATGATCACAAATGTTCTAGCACATACTAAGGGTGTGTTTGAAATTACTTTCCAAGTGTTTTTTAGCACTTAAAATGTCATTTGAAACACTCTCGTTCGGACTTCGCTTGAAAATGTTGAGTTGTTGTTACGTTGTTGTATTTAGTGGCTTCAAAAGATACTAACATGGATATGAAAGAATGATCCATGTTGTAGTAGTACTGTAAATATTTATGTAGTAAAGAACATCTCCCTGTTAGGAATCATGACCCTCTATGATGGTATGATATTGTCCACTTTGAGCGTAAGCTCTCATGGTTTTGCTTTTGGTTTTCCCAAACATCCTTACTTATAAACCCATGATCTTCCTTTTATTTAGCTAATGTGGGACTACTCCTTTCAATAATCGTCAACAATCCTCCCCTCAAACGAGTTACACCATAGAGCCTCCCCTTAGGCCTATGAAGCCCTAAAACAACCTCCCCTTAATCAAGGTTTGACTTCTTTCTCTGGAGCCCTCGAACAAAGTACACCCTTCGTTCGACACTTGAGTCACTTTTGACTACACCTTCGAGGCTCACAACTTCTTTGTTCGACATTTGAGGATTCTATTGACATGACTAAGTTAAGGGCATGGCTTTGATACCATATTAGGAAACACGACCCTCCACAATGGTATGATATTGTCTGCTTTGAGCATAAGATCTCGTGGTTTTGCTTTTGGTTTCCCCAAAATAACTCATAACAATGGAGATAGTGTTCCTTGGTTATAAACTCATGATTTTCCTATTAATTAGCCGATGTAGGACTACTCCTTTCAATAATCCTCGATACTCCCAAGTTTAGATCGTACTCTTCCTTATTGTGTTGTTTTATTTTGATTTGCTTTTAGGGTAGCCAACAACATGATGTGTTGAATGAAATATCTTACTGTAACTGTTTGGAAATTCTATGAGTCTGCAGCGTTTGGAGGAGATGGGAGTGTACAACATTCCTCTGGAGGAAGGAAAGAAACGATTTGATGTCGTTAACCCAGGAGATGTTGTGATTTTGCCTGCCTTTGGGGCTGCAGTTGATGAAATGCTTGTTCTGAATGACAAAAAAGTTAAGATTGTTGACACAACTTGCCCATGGGTTTCTAAGGTACTCCATCATTTTCTTGTCATTCACAGTCCCTCCGAGTACCATCATTGAAGGAAAGTAAGGGCACAGTGTAGCTGTTGATGCTGAGATTACCTCTGCATTTCTTTTGTATAGGTTTGGAATGCTGTTGACAAGCACAAAAAAGGAGAATACACGTCAATAATTCACGGCAAGTATCGTCACGAAGAAACCGTAGCAACTGCTTCGTTCGCTGGAAAGTACATCATTGTGAAAGACATAGTAGAGGTAAATCCTTGGTACTTTTGGCTCATAGCATAAGGAAGCATTTTAAAACTAAAATCTGAACCGATGTCAGGCGACGTATGTATGCGACTACATTCTTGGGGTTGAAAGTAATGGATTGATCTCAACTAAAGAAGCTTTTTTAAAGGTACAACATGTTAATGATTTGCTTAATGGAACTTGCTGATACTTTACGATATCGTCGCTCATGATAATAGTCGAGTTTCTGGCTTGTTTGTAGAAATTTAAGTCTGCTGTTTCAAAAGGATTCGACCCAGATAGTGATCTCATGAAAGTTGGCATTGCTAATCAGACCACAATGCTCAAAGGGGAAACAGAAGAGATTGGTAAATTTTTGGAGCCATTGATTTGTTAGAAACTCGCAATCTGTGAATCATTGATCTTATGATTCTGATAACAGGAAAATTGATCGAGAAAACTATGATGCGGAAGTATGGAATTGAAAATATTAATGATCACTTCATTAGTTTCAATACGATCTGTAATGCTACTCAAGTAAGTTCTACATGTTAAAGTTGTCGTGTAAAGTGTTGATATCGTTTGAAACGGATGAGATGGCAAGTTTACTGCTAGTAAGGACCTCGTGTCCGTTGATAATAGGCCGAACTTTCTCGTAATTTACAAAATTATGTATTTACTCGCAATAAAATCTCGTGATGGTGATTGGAGGTGGTGTTTTTCATTAGGTGCGTCAAGATGCCATGTACCAGCTGGTGGAGGAAGATATGGATCTCATGTTAGTTGTTGGAGGATGGAACTCAAGCAACACCTCTCACCTTCAAGAGATTGCAGAGGCTCGAGGAATCCCGTCGTACTGGATCGACAGCGAGCAACGTATAGGACCAGGAAACTCGATATCATACAAGCTCAATGTAAGTATGAATTTAGAATGTTGATCCAAAGACTACTTGGCATTTGTTTTTCTTTTATTGTGAGATCCCAGGTTGGTTGGAGTGGGAAACAAAACATTCTTTTTAAGGGTATGGAAACCTCTCCGTAGAAGATGCGTTTTAAAAACCTTGAAGGGAAGCCCATAAGGGAAAACCCAAAAAGGATAGTATCTGCTAGCGGTGGGCTTGGGCCGTTGCAAATAGTATCAGAGTCAAACACTGGGCGATTTTTCAGTAAGGAGGTTGAACCCCGAAGGGGGGGTGGACACCAAGCGGTGTGTCAGCGAGGATGATGGGTCCCGAAAGAGGGTGGATTGCAAGATCCCACATCGGTTGGAGAGGGGAACAATACATTCTTTATAAGGGTGCAGAAACCTCTCCCTAGCAGACGCGTTTGAAGTCCTTGAGGGGAAGCCCAGAAGGGAAAGCCCAAAGAGGACAATATCTGCTAGCTGTGGGCTTGGATCGTTACATTTATACTCATGTCAACTCGTTGAATCGCACGACAGCACGGGGAGCTGGCGAAGAAAGAAAACTGGCTACCTGATGGACATGTTACCATTGGAGTGACCTCCGGTGCCTCTACACCAGATAAGGTAAGTTAAAAGTTGCTGTCAAACCTTGTCTGTGACTGTAAATTGTCGATCCGCCATTAACTTTTGTGAAATGGGTTAAAAATTACAGGTTGTTGGAAATGTTCTTAAGAAGGTTTTCGAGATCAAAGGGGAAGGAACTGCGCAGCTTGAATAGCTTGGACAAAAGCAAACTACAGAGTATAGCGTTACTGAACTGTTTTCTTTTCCTTTCTTTTCCATTTTTGTTTGCTAGATTATGTTTATGATATTAAAAATAGTGACGTAATAAATAAACTTCACACATTTATCATATAAAA

mRNA sequence

TGAGGGAAATAAAGTTTAAAGTTCTTCCAAAAAAGTTCGATGTTTTTCACTCGTAGTCGTTCCATGCTTTCAACCACCCACCATCTCTGCTAATCGGAGGAGGTAATCTGTAAAGCAGGATCAAGATTCGGTATACCTGTTCATTGATTTCAGTATTTTAATGAGTAGAACTTTTCCGGATTTGGACTTTGAATTTGAAACGTAATTTACGAAGCACCAGCAATGCTGTGCGTTCATATTTCTACGTCAACGTCCATTCTTCGTTGTTGTTATACCACCGTTTTCCATGGCGATTTCTCTGCAGATCCCCCGTCTTCCCCGTCGTGTAGACTCTGTTTCATCGAAGAGTTTTTCCGGCGCCGGAGCTCTCCGGTTCCGGAAACCCCTTTTGGTTCGGTGCACTGCTGATTCTTCGTCGGTCAATCCCGATTTCGATGCGAAGCTGTTCTGGAAGAACTTTTCCAGAGGTGAAAACTATAATCGGAAAGGGTTTGGGTATAAGGAGGAGGCGTTGAAGCTCATGAGTAGCGAGTTCACAAGTGAAGTTGTGAATACTCTGAATGAAAATGGGAATCGGTTCACTTGGGGGAATGTTACTGTCAAATTGGCTGGAGCTTATGGCTTTTGTTGGGGAGTGGAACGAGCTGTGCAGATTGCTTATGAAGCGAGGAAACAGTTTCCGAGTGAAACAATATGGATTACCAATGAGATTATTCATAACCCAACTGTTAACAAGCGTTTGGAGGAGATGGGAGTGTACAACATTCCTCTGGAGGAAGGAAAGAAACGATTTGATGTCGTTAACCCAGGAGATGTTGTGATTTTGCCTGCCTTTGGGGCTGCAGTTGATGAAATGCTTGTTCTGAATGACAAAAAAGTTAAGATTGTTGACACAACTTGCCCATGGGTTTCTAAGGTTTGGAATGCTGTTGACAAGCACAAAAAAGGAGAATACACGTCAATAATTCACGGCAAGTATCGTCACGAAGAAACCGTAGCAACTGCTTCGTTCGCTGGAAAGTACATCATTGTGAAAGACATAGTAGAGGCGACGTATGTATGCGACTACATTCTTGGGGTTGAAAGTAATGGATTGATCTCAACTAAAGAAGCTTTTTTAAAGAAATTTAAGTCTGCTGTTTCAAAAGGATTCGACCCAGATAGTGATCTCATGAAAGTTGGCATTGCTAATCAGACCACAATGCTCAAAGGGGAAACAGAAGAGATTGGAAAATTGATCGAGAAAACTATGATGCGGAAGTATGGAATTGAAAATATTAATGATCACTTCATTAGTTTCAATACGATCTGTAATGCTACTCAAGTGCGTCAAGATGCCATGTACCAGCTGGTGGAGGAAGATATGGATCTCATGTTAGTTGTTGGAGGATGGAACTCAAGCAACACCTCTCACCTTCAAGAGATTGCAGAGGCTCGAGGAATCCCGTCGTACTGGATCGACAGCGAGCAACGTATAGGACCAGGAAACTCGATATCATACAAGCTCAATAAAGAAAACTGGCTACCTGATGGACATGTTACCATTGGAGTGACCTCCGGTGCCTCTACACCAGATAAGGTTGTTGGAAATGTTCTTAAGAAGGTTTTCGAGATCAAAGGGGAAGGAACTGCGCAGCTTGAATAGCTTGGACAAAAGCAAACTACAGAGTATAGCGTTACTGAACTGTTTTCTTTTCCTTTCTTTTCCATTTTTGTTTGCTAGATTATGTTTATGATATTAAAAATAGTGACGTAATAAATAAACTTCACACATTTATCATATAAAA

Coding sequence (CDS)

ATGGCGATTTCTCTGCAGATCCCCCGTCTTCCCCGTCGTGTAGACTCTGTTTCATCGAAGAGTTTTTCCGGCGCCGGAGCTCTCCGGTTCCGGAAACCCCTTTTGGTTCGGTGCACTGCTGATTCTTCGTCGGTCAATCCCGATTTCGATGCGAAGCTGTTCTGGAAGAACTTTTCCAGAGGTGAAAACTATAATCGGAAAGGGTTTGGGTATAAGGAGGAGGCGTTGAAGCTCATGAGTAGCGAGTTCACAAGTGAAGTTGTGAATACTCTGAATGAAAATGGGAATCGGTTCACTTGGGGGAATGTTACTGTCAAATTGGCTGGAGCTTATGGCTTTTGTTGGGGAGTGGAACGAGCTGTGCAGATTGCTTATGAAGCGAGGAAACAGTTTCCGAGTGAAACAATATGGATTACCAATGAGATTATTCATAACCCAACTGTTAACAAGCGTTTGGAGGAGATGGGAGTGTACAACATTCCTCTGGAGGAAGGAAAGAAACGATTTGATGTCGTTAACCCAGGAGATGTTGTGATTTTGCCTGCCTTTGGGGCTGCAGTTGATGAAATGCTTGTTCTGAATGACAAAAAAGTTAAGATTGTTGACACAACTTGCCCATGGGTTTCTAAGGTTTGGAATGCTGTTGACAAGCACAAAAAAGGAGAATACACGTCAATAATTCACGGCAAGTATCGTCACGAAGAAACCGTAGCAACTGCTTCGTTCGCTGGAAAGTACATCATTGTGAAAGACATAGTAGAGGCGACGTATGTATGCGACTACATTCTTGGGGTTGAAAGTAATGGATTGATCTCAACTAAAGAAGCTTTTTTAAAGAAATTTAAGTCTGCTGTTTCAAAAGGATTCGACCCAGATAGTGATCTCATGAAAGTTGGCATTGCTAATCAGACCACAATGCTCAAAGGGGAAACAGAAGAGATTGGAAAATTGATCGAGAAAACTATGATGCGGAAGTATGGAATTGAAAATATTAATGATCACTTCATTAGTTTCAATACGATCTGTAATGCTACTCAAGTGCGTCAAGATGCCATGTACCAGCTGGTGGAGGAAGATATGGATCTCATGTTAGTTGTTGGAGGATGGAACTCAAGCAACACCTCTCACCTTCAAGAGATTGCAGAGGCTCGAGGAATCCCGTCGTACTGGATCGACAGCGAGCAACGTATAGGACCAGGAAACTCGATATCATACAAGCTCAATAAAGAAAACTGGCTACCTGATGGACATGTTACCATTGGAGTGACCTCCGGTGCCTCTACACCAGATAAGGTTGTTGGAAATGTTCTTAAGAAGGTTTTCGAGATCAAAGGGGAAGGAACTGCGCAGCTTGAATAG

Protein sequence

MAISLQIPRLPRRVDSVSSKSFSGAGALRFRKPLLVRCTADSSSVNPDFDAKLFWKNFSRGENYNRKGFGYKEEALKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVILPAFGAAVDEMLVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATASFAGKYIIVKDIVEATYVCDYILGVESNGLISTKEAFLKKFKSAVSKGFDPDSDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNKENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEIKGEGTAQLE
Homology
BLAST of Cp4.1LG16g02280 vs. ExPASy Swiss-Prot
Match: Q94B35 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISPH PE=1 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 7.9e-191
Identity = 332/465 (71.40%), Postives = 390/465 (83.87%), Query Frame = 0

Query: 1   MAISLQIPRLPRRVDS-VSSKSFSGAGALRFRKPLLVRCTA-DSSSVNP------DFDAK 60
           MA++LQ  RL  R D+ V     SG+G+LR RK L VRC++ D ++ +P      DFDAK
Sbjct: 1   MAVALQFSRLCVRPDTFVRENHLSGSGSLRRRKALSVRCSSGDENAPSPSVVMDSDFDAK 60

Query: 61  LFWKNFSRGENYNRKGFGYKEEALKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYG 120
           +F KN +R +NYNRKGFG+KEE LKLM+ E+TS+++ TL  NG  ++WG+VTVKLA AYG
Sbjct: 61  VFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKLAKAYG 120

Query: 121 FCWGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVV 180
           FCWGVERAVQIAYEARKQFP E +WITNEIIHNPTVNKRLE+M V  IP+E+ KK+FDVV
Sbjct: 121 FCWGVERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRLEDMDVKIIPVEDSKKQFDVV 180

Query: 181 NPGDVVILPAFGAAVDEMLVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYR 240
              DVVILPAFGA VDEM VLNDKKV+IVDTTCPWV+KVWN V+KHKKGEYTS+IHGKY 
Sbjct: 181 EKDDVVILPAFGAGVDEMYVLNDKKVQIVDTTCPWVTKVWNTVEKHKKGEYTSVIHGKYN 240

Query: 241 HEETVATASFAGKYIIVKDIVEATYVCDYILGVESNGLISTKEAFLKKFKSAVSKGFDPD 300
           HEET+ATASFAGKYIIVK++ EA YVCDYILG + +G  STKE F++KFK A+SKGFDPD
Sbjct: 241 HEETIATASFAGKYIIVKNMKEANYVCDYILGGQYDGSSSTKEEFMEKFKYAISKGFDPD 300

Query: 301 SDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAM 360
           +DL+KVGIANQTTMLKGETEEIG+L+E TMMRKYG+EN++ HFISFNTIC+ATQ RQDA+
Sbjct: 301 NDLVKVGIANQTTMLKGETEEIGRLLETTMMRKYGVENVSGHFISFNTICDATQERQDAI 360

Query: 361 YQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLN---- 420
           Y+LVEE +DLMLVVGGWNSSNTSHLQEI+EARGIPSYWIDSE+RIGPGN I+YKL+    
Sbjct: 361 YELVEEKIDLMLVVGGWNSSNTSHLQEISEARGIPSYWIDSEKRIGPGNKIAYKLHYGEL 420

Query: 421 --KENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEIKGEGTAQL 452
             KEN+LP G +TIGVTSGASTPDKVV + L KVF+IK E   QL
Sbjct: 421 VEKENFLPKGPITIGVTSGASTPDKVVEDALVKVFDIKREELLQL 465

BLAST of Cp4.1LG16g02280 vs. ExPASy Swiss-Prot
Match: Q6AVG6 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISPH PE=2 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 1.4e-187
Identity = 321/433 (74.13%), Postives = 367/433 (84.76%), Query Frame = 0

Query: 31  RKPLLVRCTAD-------SSSVNPDFDAKLFWKNFSRGENYNRKGFGYKEEALKLMSSEF 90
           R P  VRC +        S+S++ DFD K F  N +R +NYNRKGFG+K+E L+LMS E+
Sbjct: 25  RAPSSVRCDSSAASSLSASASLDADFDKKQFRHNLTRSDNYNRKGFGHKKETLELMSQEY 84

Query: 91  TSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERAVQIAYEARKQFPSETIWITNEII 150
           TS+V+ TL ENGN+ TWG VTVKLA AYGFCWGVERAVQIAYEARKQFP + IW+TNEII
Sbjct: 85  TSDVIKTLKENGNQHTWGPVTVKLAEAYGFCWGVERAVQIAYEARKQFPDDRIWLTNEII 144

Query: 151 HNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVILPAFGAAVDEMLVLNDKKVKIVDT 210
           HNPTVNKRLE+MGV NIP++ G K FDVV  GDVV+LPAFGAAV+EM  LN+KKV+IVDT
Sbjct: 145 HNPTVNKRLEDMGVQNIPVDAGIKDFDVVEQGDVVVLPAFGAAVEEMYTLNEKKVQIVDT 204

Query: 211 TCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATASFAGKYIIVKDIVEATYVCDYIL 270
           TCPWVSKVWN V+KHKKG+YTSIIHGKY HEETVATASFAG YIIVK+I EA+YVCDYIL
Sbjct: 205 TCPWVSKVWNMVEKHKKGDYTSIIHGKYSHEETVATASFAGTYIIVKNIAEASYVCDYIL 264

Query: 271 GVESNGLISTKEAFLKKFKSAVSKGFDPDSDLMKVGIANQTTMLKGETEEIGKLIEKTMM 330
           G + +G  STKE FL+KFK+AVS GFDPD DL+KVGIANQTTMLKGETEEIGKL+EKTMM
Sbjct: 265 GGQLDGSSSTKEEFLEKFKNAVSPGFDPDVDLVKVGIANQTTMLKGETEEIGKLVEKTMM 324

Query: 331 RKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEA 390
           R++G+EN+NDHFI+FNTIC+ATQ RQDAMYQLV+E +DL+LVVGGWNSSNTSHLQEI E 
Sbjct: 325 RRFGVENVNDHFIAFNTICDATQERQDAMYQLVKEKVDLILVVGGWNSSNTSHLQEIGEL 384

Query: 391 RGIPSYWIDSEQRIGPGNSISYKLN------KENWLPDGHVTIGVTSGASTPDKVVGNVL 450
            GIPSYWIDSEQRIGPGN ISYKLN      KENWLP+G +TIGVTSGASTPDKVV + L
Sbjct: 385 SGIPSYWIDSEQRIGPGNKISYKLNHGELVEKENWLPEGPITIGVTSGASTPDKVVEDAL 444

BLAST of Cp4.1LG16g02280 vs. ExPASy Swiss-Prot
Match: B8HWD3 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) OX=395961 GN=ispH PE=3 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 1.1e-142
Identity = 250/407 (61.43%), Postives = 313/407 (76.90%), Query Frame = 0

Query: 50  DAKLFWKNFSRGENYNRKGFGYKEEALKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAG 109
           D K F ++      Y+RKGFG++ E  +++  E+ S ++  + ENGNRF  G VT++LA 
Sbjct: 2   DTKAFKRSLHSSNQYHRKGFGHEAEVTEMLRFEYQSSLIQQIRENGNRFQRGEVTIQLAE 61

Query: 110 AYGFCWGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPL-EEGKKR 169
           A+GFCWGVERAV +AYE R+ FP+E IWITNEIIHNP+VN+ L EM V  IP+ E+G+K 
Sbjct: 62  AFGFCWGVERAVALAYETRQHFPTERIWITNEIIHNPSVNQNLREMQVEFIPVDEQGQKD 121

Query: 170 FDVVNPGDVVILPAFGAAVDEMLVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIH 229
           F VV   DVVILPAFGA+V EM +LNDK  KI+DTTCPWVSKVWN+V+KHKKG YTSIIH
Sbjct: 122 FSVVAGEDVVILPAFGASVQEMQLLNDKGCKIMDTTCPWVSKVWNSVEKHKKGSYTSIIH 181

Query: 230 GKYRHEETVATASFAGKYIIVKDIVEATYVCDYILGVESNGLISTKEAFLKKFKSAVSKG 289
           GKY+HEET+AT+S+A  Y+IV D+ EA YVC+YIL    NG    +E FL KF+ A S  
Sbjct: 182 GKYQHEETIATSSYAATYLIVLDLKEAEYVCNYIL----NG--GDREEFLHKFRHAYSPD 241

Query: 290 FDPDSDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVR 349
           FDPD DL++VGIANQTTMLKGETE+IGKL E+T+MRKYG + IN HF+SFNTIC+ATQ R
Sbjct: 242 FDPDRDLVRVGIANQTTMLKGETEQIGKLFERTLMRKYGPDQINQHFLSFNTICDATQER 301

Query: 350 QDAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYK-- 409
           QDAM++LVE  +DLM+V+GG+NSSNT+HLQEI+  R IPSY IDS  RIGPGN + +K  
Sbjct: 302 QDAMFKLVEYPLDLMVVIGGFNSSNTTHLQEISIEREIPSYHIDSADRIGPGNRVEHKPL 361

Query: 410 ----LNKENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEIKGEGTA 450
                 KENWLP+G + +G+TSGASTPDKVV +VL+K+F +K    A
Sbjct: 362 HRPLEIKENWLPEGPIVVGITSGASTPDKVVSDVLEKIFALKSTSPA 402

BLAST of Cp4.1LG16g02280 vs. ExPASy Swiss-Prot
Match: B1WTZ2 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Crocosphaera subtropica (strain ATCC 51142 / BH68) OX=43989 GN=ispH PE=3 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 6.8e-142
Identity = 248/404 (61.39%), Postives = 314/404 (77.72%), Query Frame = 0

Query: 50  DAKLFWKNFSRGENYNRKGFGYKEEALKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAG 109
           D K F ++  +  NY+RKGFG++ E +  M++E+ S ++  + EN  ++  G+VT+KLA 
Sbjct: 2   DTKAFKRSLQQSNNYHRKGFGHETEVMGTMNTEYQSSLIQKIRENNYQWQEGDVTIKLAE 61

Query: 110 AYGFCWGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRF 169
           A+GFCWGVERAV +AYE R+ FP E IWITNEIIHNP+VN+RL EM V  I +  G K F
Sbjct: 62  AFGFCWGVERAVAMAYETRQHFPQEKIWITNEIIHNPSVNQRLLEMNVGFIEVINGSKDF 121

Query: 170 DVVNPGDVVILPAFGAAVDEMLVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHG 229
            VV  GDVVILPAFGA+V EM +LNDK   IVDTTCPWVSKVWN+V+KHKK +YTSIIHG
Sbjct: 122 SVVEAGDVVILPAFGASVTEMQLLNDKGCTIVDTTCPWVSKVWNSVEKHKKKDYTSIIHG 181

Query: 230 KYRHEETVATASFAGKYIIVKDIVEATYVCDYILGVESNGLISTKEAFLKKFKSAVSKGF 289
           KY+HEETVAT+SFAG Y++V ++ EA YVCDYIL    NG    K+ FL+KFK+A S+GF
Sbjct: 182 KYKHEETVATSSFAGTYLVVLNLKEAQYVCDYIL----NG--GNKDEFLEKFKNAHSEGF 241

Query: 290 DPDSDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQ 349
           DPD DL+++GIANQTTMLK ETE+IGKL E TM++KYG   +N+HF+SFNTIC+ATQ RQ
Sbjct: 242 DPDKDLIRLGIANQTTMLKSETEQIGKLFETTMLKKYGPTELNEHFMSFNTICDATQERQ 301

Query: 350 DAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYK--- 409
           DAM  LVEE++D+M+V+GG+NSSNT+HLQEI+  +GI SY IDS++RI PGN I +K   
Sbjct: 302 DAMLNLVEEEVDVMVVIGGFNSSNTTHLQEISIEKGIDSYHIDSDKRILPGNKIEHKPLE 361

Query: 410 ---LNKENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEIKGEG 448
                KENWLPDG + +GVTSGASTPDKVV  V++++FE K  G
Sbjct: 362 KDIETKENWLPDGKIIVGVTSGASTPDKVVEAVIERIFEEKNAG 399

BLAST of Cp4.1LG16g02280 vs. ExPASy Swiss-Prot
Match: Q8DK29 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=ispH PE=3 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 8.3e-140
Identity = 245/405 (60.49%), Postives = 306/405 (75.56%), Query Frame = 0

Query: 50  DAKLFWKNFSRGENYNRKGFGYKEEALKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAG 109
           D + F ++    ENY+RKGFG+ EE  + +  E+ S ++  +  NG R+  G+VT++LA 
Sbjct: 2   DTRAFKRSLHSSENYHRKGFGHGEEVNQQLQGEYQSSLIQQIRANGYRWQQGDVTIRLAE 61

Query: 110 AYGFCWGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRF 169
           A+GFCWGVERAV +AYE R  FP+E IWITNEIIHNP+VN+RL +M V  IP+  G K F
Sbjct: 62  AFGFCWGVERAVALAYETRTHFPTERIWITNEIIHNPSVNERLRQMAVEFIPVVNGVKDF 121

Query: 170 DVVNPGDVVILPAFGAAVDEMLVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHG 229
             V PGDVVILPAFGA+V EM +LN++   IVDTTCPWVSKVW++V+KHKK  +TSIIHG
Sbjct: 122 SGVRPGDVVILPAFGASVQEMQLLNERGCTIVDTTCPWVSKVWHSVEKHKKVSFTSIIHG 181

Query: 230 KYRHEETVATASFAGKYIIVKDIVEATYVCDYILGVESNGLISTKEAFLKKFKSAVSKGF 289
           KY HEET+AT+SFAG Y+IV ++ EA YVCDYIL    +G    + AF+ KF  A S GF
Sbjct: 182 KYNHEETIATSSFAGTYLIVLNLEEARYVCDYIL----HG--GDRAAFMAKFAKACSPGF 241

Query: 290 DPDSDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQ 349
           DPD DL++VGIANQTTMLKGETE+IGKL E+TM++KYG + +N+HF+SFNTIC+ATQ RQ
Sbjct: 242 DPDRDLVRVGIANQTTMLKGETEQIGKLFERTMIQKYGPDRLNEHFMSFNTICDATQERQ 301

Query: 350 DAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYK--- 409
           DAM  LV+E +DLM+V+GG+NSSNT+HLQEIA   GIPSY IDS  RIGPGN I +K   
Sbjct: 302 DAMLSLVKEPLDLMVVIGGYNSSNTTHLQEIAIEHGIPSYHIDSADRIGPGNRIEHKPLH 361

Query: 410 ---LNKENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEIKGEGT 449
                 ENWLPD  +TIG+TSGASTPDKVV  VL K+F ++   T
Sbjct: 362 QNPTVAENWLPDRPITIGITSGASTPDKVVEEVLNKIFALRSVAT 400

BLAST of Cp4.1LG16g02280 vs. NCBI nr
Match: XP_023512039.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 909 bits (2350), Expect = 0.0
Identity = 452/458 (98.69%), Postives = 452/458 (98.69%), Query Frame = 0

Query: 1   MAISLQIPRLPRRVDSVSSKSFSGAGALRFRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60
           MAISLQIPRLPRRVDSVSSKSFSGAGALRFRKPLLVRCTADSSSVNPDFDAKLFWKNFSR
Sbjct: 1   MAISLQIPRLPRRVDSVSSKSFSGAGALRFRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60

Query: 61  GENYNRKGFGYKEEALKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120
           GENYNRKGFGYKEEALKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA
Sbjct: 61  GENYNRKGFGYKEEALKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120

Query: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180
           VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL
Sbjct: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180

Query: 181 PAFGAAVDEMLVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240
           PAFGAAVDEMLVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA
Sbjct: 181 PAFGAAVDEMLVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240

Query: 241 SFAGKYIIVKDIVEATYVCDYILGVESNGLISTKEAFLKKFKSAVSKGFDPDSDLMKVGI 300
           SFAGKYIIVKDIVEATYVCDYILGVESNGLISTKEAFLKKFKSAVSKGFDPDSDLMKVGI
Sbjct: 241 SFAGKYIIVKDIVEATYVCDYILGVESNGLISTKEAFLKKFKSAVSKGFDPDSDLMKVGI 300

Query: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360
           ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM
Sbjct: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360

Query: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLN------KENWLP 420
           DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLN      KENWLP
Sbjct: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLP 420

Query: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEIKGEGTAQLE 452
           DGHVTIGVTSGASTPDKVVGNVLKKVFEIKGEGTAQLE
Sbjct: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEIKGEGTAQLE 458

BLAST of Cp4.1LG16g02280 vs. NCBI nr
Match: KAG7010948.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 887 bits (2291), Expect = 0.0
Identity = 440/458 (96.07%), Postives = 446/458 (97.38%), Query Frame = 0

Query: 1   MAISLQIPRLPRRVDSVSSKSFSGAGALRFRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60
           MAISLQIPRL  RVDSVSSK FSG GALR RKPLLVRCTADSSSVNPDFDAKLFWKNFSR
Sbjct: 1   MAISLQIPRLHLRVDSVSSKRFSGGGALRCRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60

Query: 61  GENYNRKGFGYKEEALKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120
           GENYNRKGFG+KEE LKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA
Sbjct: 61  GENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120

Query: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180
           VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL
Sbjct: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180

Query: 181 PAFGAAVDEMLVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240
           PAFGAAVDEMLVL+DKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA
Sbjct: 181 PAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240

Query: 241 SFAGKYIIVKDIVEATYVCDYILGVESNGLISTKEAFLKKFKSAVSKGFDPDSDLMKVGI 300
           SFAGKYIIVKD+VEATYVCDYILGVESNGLISTKEAFLKKFKSA+SKGFDPDSDLMKVGI
Sbjct: 241 SFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGI 300

Query: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360
           ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM
Sbjct: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360

Query: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLN------KENWLP 420
           DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLN      KENWLP
Sbjct: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLP 420

Query: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEIKGEGTAQLE 452
           DGHVTIGVTSGASTPDKVVGNVLKKVFE+KGEGTAQ+E
Sbjct: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQVE 458

BLAST of Cp4.1LG16g02280 vs. NCBI nr
Match: XP_022944389.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 884 bits (2285), Expect = 0.0
Identity = 439/458 (95.85%), Postives = 445/458 (97.16%), Query Frame = 0

Query: 1   MAISLQIPRLPRRVDSVSSKSFSGAGALRFRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60
           MAISLQIPRL  RVDSVSSK FS  GALR RKPLLVRCTADSSSVNPDFDAKLFWKNFSR
Sbjct: 1   MAISLQIPRLHLRVDSVSSKRFSAGGALRCRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60

Query: 61  GENYNRKGFGYKEEALKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120
           GENYNRKGFG+KEE LKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA
Sbjct: 61  GENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120

Query: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180
           VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL
Sbjct: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180

Query: 181 PAFGAAVDEMLVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240
           PAFGAAVDEMLVL+DKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA
Sbjct: 181 PAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240

Query: 241 SFAGKYIIVKDIVEATYVCDYILGVESNGLISTKEAFLKKFKSAVSKGFDPDSDLMKVGI 300
           SFAGKYIIVKD+VEATYVCDYILGVESNGLISTKEAFLKKFKSA+SKGFDPDSDLMKVGI
Sbjct: 241 SFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGI 300

Query: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360
           ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM
Sbjct: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360

Query: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLN------KENWLP 420
           DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLN      KENWLP
Sbjct: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLP 420

Query: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEIKGEGTAQLE 452
           DGHVTIGVTSGASTPDKVVGNVLKKVFE+KGEGTAQ+E
Sbjct: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQVE 458

BLAST of Cp4.1LG16g02280 vs. NCBI nr
Match: KAG6571137.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 884 bits (2283), Expect = 0.0
Identity = 438/458 (95.63%), Postives = 444/458 (96.94%), Query Frame = 0

Query: 1   MAISLQIPRLPRRVDSVSSKSFSGAGALRFRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60
           MAISLQIPRL  RVDSVSSK FSG GALR RKPLLVRCTADSSSVNPDFDAKLFWKNFSR
Sbjct: 1   MAISLQIPRLHLRVDSVSSKRFSGGGALRCRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60

Query: 61  GENYNRKGFGYKEEALKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120
           GENYNRKGFG+KEE LKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA
Sbjct: 61  GENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120

Query: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180
           VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL
Sbjct: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180

Query: 181 PAFGAAVDEMLVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240
           PAFGAAVDEMLVL+DKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA
Sbjct: 181 PAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240

Query: 241 SFAGKYIIVKDIVEATYVCDYILGVESNGLISTKEAFLKKFKSAVSKGFDPDSDLMKVGI 300
           SFAGKYI VKD+ EATYVCDYILGVESNGLISTKEAFLKKFKSA+SKGFDPDSDLMKVGI
Sbjct: 241 SFAGKYIFVKDMAEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGI 300

Query: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360
           ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM
Sbjct: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360

Query: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLN------KENWLP 420
           DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLN      KENWLP
Sbjct: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLP 420

Query: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEIKGEGTAQLE 452
           DGHVTIGVTSGASTPDKVVGNVLKKVFE+KGEGTAQ+E
Sbjct: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQVE 458

BLAST of Cp4.1LG16g02280 vs. NCBI nr
Match: XP_022986524.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 866 bits (2238), Expect = 0.0
Identity = 431/458 (94.10%), Postives = 439/458 (95.85%), Query Frame = 0

Query: 1   MAISLQIPRLPRRVDSVSSKSFSGAGALRFRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60
           MAISLQIPRLP RVDSVSSK FSG GALR  KPLLVRCTADSSSVNPDFDAKLFWKNFSR
Sbjct: 1   MAISLQIPRLPLRVDSVSSKRFSGGGALRCWKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60

Query: 61  GENYNRKGFGYKEEALKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120
           GENYNRKGF +KEE LKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA
Sbjct: 61  GENYNRKGFEHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120

Query: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180
           VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL
Sbjct: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180

Query: 181 PAFGAAVDEMLVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240
           PAFGAAVDEMLVL+DKKV IVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKY HEETVATA
Sbjct: 181 PAFGAAVDEMLVLSDKKVTIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYHHEETVATA 240

Query: 241 SFAGKYIIVKDIVEATYVCDYILGVESNGLISTKEAFLKKFKSAVSKGFDPDSDLMKVGI 300
           SFAGKYIIVKD+VEATYVCDYILGVESNGLISTKE FLKKFKSAVSKGFDPDSDLMKVGI
Sbjct: 241 SFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEDFLKKFKSAVSKGFDPDSDLMKVGI 300

Query: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360
           ANQTTMLK ET+EIGKLIEKTMMRKYGIENI++HFISFNTICNATQVRQDAMYQLVEED 
Sbjct: 301 ANQTTMLKEETKEIGKLIEKTMMRKYGIENISEHFISFNTICNATQVRQDAMYQLVEEDT 360

Query: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLN------KENWLP 420
           DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKL       KENWLP
Sbjct: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLKHGELVKKENWLP 420

Query: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEIKGEGTAQLE 452
           DGH+TIGVTSGASTPD+VVGNVLKKVFEIKGEGTAQLE
Sbjct: 421 DGHITIGVTSGASTPDQVVGNVLKKVFEIKGEGTAQLE 458

BLAST of Cp4.1LG16g02280 vs. ExPASy TrEMBL
Match: A0A6J1FWL7 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448851 PE=3 SV=1)

HSP 1 Score: 884 bits (2285), Expect = 0.0
Identity = 439/458 (95.85%), Postives = 445/458 (97.16%), Query Frame = 0

Query: 1   MAISLQIPRLPRRVDSVSSKSFSGAGALRFRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60
           MAISLQIPRL  RVDSVSSK FS  GALR RKPLLVRCTADSSSVNPDFDAKLFWKNFSR
Sbjct: 1   MAISLQIPRLHLRVDSVSSKRFSAGGALRCRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60

Query: 61  GENYNRKGFGYKEEALKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120
           GENYNRKGFG+KEE LKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA
Sbjct: 61  GENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120

Query: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180
           VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL
Sbjct: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180

Query: 181 PAFGAAVDEMLVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240
           PAFGAAVDEMLVL+DKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA
Sbjct: 181 PAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240

Query: 241 SFAGKYIIVKDIVEATYVCDYILGVESNGLISTKEAFLKKFKSAVSKGFDPDSDLMKVGI 300
           SFAGKYIIVKD+VEATYVCDYILGVESNGLISTKEAFLKKFKSA+SKGFDPDSDLMKVGI
Sbjct: 241 SFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGI 300

Query: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360
           ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM
Sbjct: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360

Query: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLN------KENWLP 420
           DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLN      KENWLP
Sbjct: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLP 420

Query: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEIKGEGTAQLE 452
           DGHVTIGVTSGASTPDKVVGNVLKKVFE+KGEGTAQ+E
Sbjct: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQVE 458

BLAST of Cp4.1LG16g02280 vs. ExPASy TrEMBL
Match: A0A6J1JG99 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484238 PE=3 SV=1)

HSP 1 Score: 866 bits (2238), Expect = 0.0
Identity = 431/458 (94.10%), Postives = 439/458 (95.85%), Query Frame = 0

Query: 1   MAISLQIPRLPRRVDSVSSKSFSGAGALRFRKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60
           MAISLQIPRLP RVDSVSSK FSG GALR  KPLLVRCTADSSSVNPDFDAKLFWKNFSR
Sbjct: 1   MAISLQIPRLPLRVDSVSSKRFSGGGALRCWKPLLVRCTADSSSVNPDFDAKLFWKNFSR 60

Query: 61  GENYNRKGFGYKEEALKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120
           GENYNRKGF +KEE LKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA
Sbjct: 61  GENYNRKGFEHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERA 120

Query: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180
           VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL
Sbjct: 121 VQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVIL 180

Query: 181 PAFGAAVDEMLVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATA 240
           PAFGAAVDEMLVL+DKKV IVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKY HEETVATA
Sbjct: 181 PAFGAAVDEMLVLSDKKVTIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYHHEETVATA 240

Query: 241 SFAGKYIIVKDIVEATYVCDYILGVESNGLISTKEAFLKKFKSAVSKGFDPDSDLMKVGI 300
           SFAGKYIIVKD+VEATYVCDYILGVESNGLISTKE FLKKFKSAVSKGFDPDSDLMKVGI
Sbjct: 241 SFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEDFLKKFKSAVSKGFDPDSDLMKVGI 300

Query: 301 ANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDM 360
           ANQTTMLK ET+EIGKLIEKTMMRKYGIENI++HFISFNTICNATQVRQDAMYQLVEED 
Sbjct: 301 ANQTTMLKEETKEIGKLIEKTMMRKYGIENISEHFISFNTICNATQVRQDAMYQLVEEDT 360

Query: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLN------KENWLP 420
           DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKL       KENWLP
Sbjct: 361 DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLKHGELVKKENWLP 420

Query: 421 DGHVTIGVTSGASTPDKVVGNVLKKVFEIKGEGTAQLE 452
           DGH+TIGVTSGASTPD+VVGNVLKKVFEIKGEGTAQLE
Sbjct: 421 DGHITIGVTSGASTPDQVVGNVLKKVFEIKGEGTAQLE 458

BLAST of Cp4.1LG16g02280 vs. ExPASy TrEMBL
Match: A0A1S3CAB0 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498435 PE=3 SV=1)

HSP 1 Score: 800 bits (2067), Expect = 1.35e-290
Identity = 395/463 (85.31%), Postives = 425/463 (91.79%), Query Frame = 0

Query: 1   MAISLQIPRLPRRVDSVSSKSFSGAGALRFRKPLLVRCTADSSS----VNPDFDAKLFWK 60
           MAISLQ PR P R+D    K+FSG GALR R+PLL+RCTADSSS    V+PDF+AKLF K
Sbjct: 1   MAISLQSPRFPHRIDFSLPKTFSGVGALRCRQPLLLRCTADSSSSPKPVDPDFNAKLFLK 60

Query: 61  NFSRGENYNRKGFGYKEEALKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWG 120
           NFSRGENYNRKGFG+KEE +KLM+SEFTSE+V TL ENG +FTWGNVTVKLAGAYGFCWG
Sbjct: 61  NFSRGENYNRKGFGHKEETVKLMNSEFTSEIVTTLKENGFQFTWGNVTVKLAGAYGFCWG 120

Query: 121 VERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEG-KKRFDVVNPG 180
           VERAVQ AYEARKQ+PSETIWITNEIIHNPTVNKRLEEMGV+NIPLEEG KKRFD VNPG
Sbjct: 121 VERAVQFAYEARKQYPSETIWITNEIIHNPTVNKRLEEMGVHNIPLEEGNKKRFDAVNPG 180

Query: 181 DVVILPAFGAAVDEMLVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEE 240
           DVVILPAFGAAVDE+LVLNDKKVKIVDTTCPWVSKVWNAV KHKKGEYTSIIHGKY HEE
Sbjct: 181 DVVILPAFGAAVDEILVLNDKKVKIVDTTCPWVSKVWNAVGKHKKGEYTSIIHGKYSHEE 240

Query: 241 TVATASFAGKYIIVKDIVEATYVCDYILGVESNGLISTKEAFLKKFKSAVSKGFDPDSDL 300
           T+ATASFAGKYIIVKD+ EA YV DYILGVE +G ISTKE FLKKFKSAVSKGFDPD  L
Sbjct: 241 TIATASFAGKYIIVKDMAEAAYVSDYILGVEHDGSISTKEEFLKKFKSAVSKGFDPDIHL 300

Query: 301 MKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQL 360
           +K+GIANQTTMLKGETEEIGKL+EKTMMRKYG+ENIN+HFISFNTICNATQVRQDAMY+L
Sbjct: 301 VKIGIANQTTMLKGETEEIGKLMEKTMMRKYGVENINEHFISFNTICNATQVRQDAMYEL 360

Query: 361 VEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKL------NK 420
           V+ED+DLMLVVGGWNSSNTSHLQEIAEARG+PSYWIDSEQRIGPGNSISYKL      NK
Sbjct: 361 VKEDVDLMLVVGGWNSSNTSHLQEIAEARGVPSYWIDSEQRIGPGNSISYKLAHGELANK 420

Query: 421 ENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEIKGEGTAQLE 452
           ENWLPDGH+TIGVTSGASTPDKVVG+VLKKVF+IKG+GTAQ E
Sbjct: 421 ENWLPDGHITIGVTSGASTPDKVVGDVLKKVFDIKGDGTAQFE 463

BLAST of Cp4.1LG16g02280 vs. ExPASy TrEMBL
Match: A0A6J1D5C2 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111017170 PE=3 SV=1)

HSP 1 Score: 776 bits (2003), Expect = 7.28e-281
Identity = 386/464 (83.19%), Postives = 417/464 (89.87%), Query Frame = 0

Query: 1   MAISLQIPRLPRRVDSVSSKSFSGAGAL--RFRKPLLVRCTADSSS----VNPDFDAKLF 60
           MAISLQIPR+P R   +S    S AGAL  R RKP  +RCTADS S    ++PDFDAKLF
Sbjct: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60

Query: 61  WKNFSRGENYNRKGFGYKEEALKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFC 120
           WKN++RGENYNRKGFG+KEE +KLM+SEFTSEV+NTLNENGN FTWGNVTVKLA AYGFC
Sbjct: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120

Query: 121 WGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNP 180
           +GVERAVQIA+EARKQFPS TIWITNEIIHNPTVNKRL EMGV+ IPLEE KK+FDVVNP
Sbjct: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP 180

Query: 181 GDVVILPAFGAAVDEMLVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHE 240
           GDVVILPAFGAAVDEMLVL DK V+IVDTTCPWVSKVWN VDKHKK EYTSIIHGKYRHE
Sbjct: 181 GDVVILPAFGAAVDEMLVLKDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE 240

Query: 241 ETVATASFAGKYIIVKDIVEATYVCDYILGVESNGLISTKEAFLKKFKSAVSKGFDPDSD 300
           ET+ATASFAGKYIIVK++ EATYV D+ILGVE NG I TKE FLKKF+SAVS+GFDPD D
Sbjct: 241 ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD 300

Query: 301 LMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQV-RQDAMY 360
           L+KVGIANQTTMLKGETEEIGKLIE+TMMRKYG+EN+N+HF+SFNTIC+ATQV RQDAMY
Sbjct: 301 LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQVKRQDAMY 360

Query: 361 QLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKL------ 420
           QLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKL      
Sbjct: 361 QLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIYGELA 420

Query: 421 NKENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEIKGEGTAQL 451
            KENWLPDGHVTIGVTSGASTPDKVV NVLKKVF++KGEGT QL
Sbjct: 421 EKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQL 464

BLAST of Cp4.1LG16g02280 vs. ExPASy TrEMBL
Match: A0A6J1FYM6 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448851 PE=3 SV=1)

HSP 1 Score: 746 bits (1927), Expect = 1.13e-270
Identity = 369/380 (97.11%), Postives = 374/380 (98.42%), Query Frame = 0

Query: 79  MSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERAVQIAYEARKQFPSETIWI 138
           MSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERAVQIAYEARKQFPSETIWI
Sbjct: 1   MSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCWGVERAVQIAYEARKQFPSETIWI 60

Query: 139 TNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVILPAFGAAVDEMLVLNDKKV 198
           TNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVILPAFGAAVDEMLVL+DKKV
Sbjct: 61  TNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPGDVVILPAFGAAVDEMLVLSDKKV 120

Query: 199 KIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATASFAGKYIIVKDIVEATYV 258
           KIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATASFAGKYIIVKD+VEATYV
Sbjct: 121 KIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHEETVATASFAGKYIIVKDMVEATYV 180

Query: 259 CDYILGVESNGLISTKEAFLKKFKSAVSKGFDPDSDLMKVGIANQTTMLKGETEEIGKLI 318
           CDYILGVESNGLISTKEAFLKKFKSA+SKGFDPDSDLMKVGIANQTTMLKGETEEIGKLI
Sbjct: 181 CDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSDLMKVGIANQTTMLKGETEEIGKLI 240

Query: 319 EKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDMDLMLVVGGWNSSNTSHLQ 378
           EKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDMDLMLVVGGWNSSNTSHLQ
Sbjct: 241 EKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQLVEEDMDLMLVVGGWNSSNTSHLQ 300

Query: 379 EIAEARGIPSYWIDSEQRIGPGNSISYKLN------KENWLPDGHVTIGVTSGASTPDKV 438
           EIAEARGIPSYWIDSEQRIGPGNSISYKLN      KENWLPDGHVTIGVTSGASTPDKV
Sbjct: 301 EIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAKKENWLPDGHVTIGVTSGASTPDKV 360

Query: 439 VGNVLKKVFEIKGEGTAQLE 452
           VGNVLKKVFE+KGEGTAQ+E
Sbjct: 361 VGNVLKKVFEMKGEGTAQVE 380

BLAST of Cp4.1LG16g02280 vs. TAIR 10
Match: AT4G34350.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase )

HSP 1 Score: 667.9 bits (1722), Expect = 5.6e-192
Identity = 332/465 (71.40%), Postives = 390/465 (83.87%), Query Frame = 0

Query: 1   MAISLQIPRLPRRVDS-VSSKSFSGAGALRFRKPLLVRCTA-DSSSVNP------DFDAK 60
           MA++LQ  RL  R D+ V     SG+G+LR RK L VRC++ D ++ +P      DFDAK
Sbjct: 1   MAVALQFSRLCVRPDTFVRENHLSGSGSLRRRKALSVRCSSGDENAPSPSVVMDSDFDAK 60

Query: 61  LFWKNFSRGENYNRKGFGYKEEALKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYG 120
           +F KN +R +NYNRKGFG+KEE LKLM+ E+TS+++ TL  NG  ++WG+VTVKLA AYG
Sbjct: 61  VFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKLAKAYG 120

Query: 121 FCWGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVV 180
           FCWGVERAVQIAYEARKQFP E +WITNEIIHNPTVNKRLE+M V  IP+E+ KK+FDVV
Sbjct: 121 FCWGVERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRLEDMDVKIIPVEDSKKQFDVV 180

Query: 181 NPGDVVILPAFGAAVDEMLVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYR 240
              DVVILPAFGA VDEM VLNDKKV+IVDTTCPWV+KVWN V+KHKKGEYTS+IHGKY 
Sbjct: 181 EKDDVVILPAFGAGVDEMYVLNDKKVQIVDTTCPWVTKVWNTVEKHKKGEYTSVIHGKYN 240

Query: 241 HEETVATASFAGKYIIVKDIVEATYVCDYILGVESNGLISTKEAFLKKFKSAVSKGFDPD 300
           HEET+ATASFAGKYIIVK++ EA YVCDYILG + +G  STKE F++KFK A+SKGFDPD
Sbjct: 241 HEETIATASFAGKYIIVKNMKEANYVCDYILGGQYDGSSSTKEEFMEKFKYAISKGFDPD 300

Query: 301 SDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAM 360
           +DL+KVGIANQTTMLKGETEEIG+L+E TMMRKYG+EN++ HFISFNTIC+ATQ RQDA+
Sbjct: 301 NDLVKVGIANQTTMLKGETEEIGRLLETTMMRKYGVENVSGHFISFNTICDATQERQDAI 360

Query: 361 YQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLN---- 420
           Y+LVEE +DLMLVVGGWNSSNTSHLQEI+EARGIPSYWIDSE+RIGPGN I+YKL+    
Sbjct: 361 YELVEEKIDLMLVVGGWNSSNTSHLQEISEARGIPSYWIDSEKRIGPGNKIAYKLHYGEL 420

Query: 421 --KENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEIKGEGTAQL 452
             KEN+LP G +TIGVTSGASTPDKVV + L KVF+IK E   QL
Sbjct: 421 VEKENFLPKGPITIGVTSGASTPDKVVEDALVKVFDIKREELLQL 465

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94B357.9e-19171.404-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis... [more]
Q6AVG61.4e-18774.134-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativ... [more]
B8HWD31.1e-14261.434-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain PCC... [more]
B1WTZ26.8e-14261.394-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Crocosphaera subtropica (s... [more]
Q8DK298.3e-14060.494-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Thermosynechococcus elonga... [more]
Match NameE-valueIdentityDescription
XP_023512039.10.098.694-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
KAG7010948.10.096.074-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic, partial [Cucu... [more]
XP_022944389.10.095.854-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
KAG6571137.10.095.634-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic, partial [Cucu... [more]
XP_022986524.10.094.104-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
Match NameE-valueIdentityDescription
A0A6J1FWL70.095.854-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
A0A6J1JG990.094.104-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
A0A1S3CAB01.35e-29085.314-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
A0A6J1D5C27.28e-28183.194-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like OS=Momord... [more]
A0A6J1FYM61.13e-27097.114-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X... [more]
Match NameE-valueIdentityDescription
AT4G34350.15.6e-19271.404-hydroxy-3-methylbut-2-enyl diphosphate reductase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.1010.20coord: 326..452
e-value: 6.4E-40
score: 137.3
NoneNo IPR availableGENE3D3.40.1010.20coord: 206..325
e-value: 2.2E-11
score: 45.4
NoneNo IPR availableGENE3D3.40.50.11270coord: 114..204
e-value: 1.2E-21
score: 78.5
NoneNo IPR availablePANTHERPTHR316194-HYDROXY-3-METHYLBUT-2-ENYL DIPHOSPHATE REDUCTASE, CHLOROPLASTICcoord: 3..448
IPR0034514-hydroxy-3-methylbut-2-enyl diphosphate reductaseTIGRFAMTIGR00216TIGR00216coord: 105..441
e-value: 8.4E-91
score: 302.0
IPR0034514-hydroxy-3-methylbut-2-enyl diphosphate reductasePFAMPF02401LYTBcoord: 105..440
e-value: 1.6E-83
score: 280.0
IPR0034514-hydroxy-3-methylbut-2-enyl diphosphate reductaseHAMAPMF_00191IspHcoord: 103..444
score: 28.962408
IPR0034514-hydroxy-3-methylbut-2-enyl diphosphate reductaseCDDcd13944lytB_ispHcoord: 105..440
e-value: 1.95276E-107
score: 317.43

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG16g02280.1Cp4.1LG16g02280.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050992 dimethylallyl diphosphate biosynthetic process
biological_process GO:0019288 isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0051745 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity
molecular_function GO:0046429 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0046872 metal ion binding