Cp4.1LG16g02150 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG16g02150
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationCp4.1LG16: 4242505 .. 4243476 (-)
RNA-Seq ExpressionCp4.1LG16g02150
SyntenyCp4.1LG16g02150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCCACCGAAATTGCCCACAACTTTTCCCCTTTGTTTATCGTTTACAAAGACGGTACCGTCCACAGGTTACTCGGCAACGATGCAATACCTCCTTCTCCGGATCCCGTTACCGGCGTCGATTCCAAAGACGTCGTCATCTCCCCGGAAACCGCTGTCTCTGTTCGGATTTACAGGCCCAAACCCGCCACCGCCACCGCACAACCCCAGAAGCTTCCTGTTCTCGTTTACGTCCACGGCGGTGGCTTCTGCATCGAATCGGCATTCTCCCCTTTCTACGACCGCCACGTCAAGTCCTTAGTTGCTGAAGCCAACGTTGTCGCCGTCTCTGTCGACTACCGTCTTGCTCCGGAGCACCCTCTTCCGATTGCTTATGAAGATTCATGGGCCGCTCTGAAATGGGTCGCTGCCCATTCCGACGGAAATGGGCCGGAGGAGTGGCTGAATGGAATCGTGGATTTGGATCGGGTTTATTTTGCTGGAGATAGCGCCGGAGCTAACATTGTTCATCACTTGGCGGTTCGGGTCGGGTCGGAGGGGATAGGCGGGTTGAATCTGAAGGGATTGATTTTGGTTCATCCTTATTTCTGGGGAGGGAAATTGGTCGGAGATGAAGAGAAGCTGAGAGCAGAGGAAAGACACTTTATGGAGAAATTGTGGTATGTGACTTGCCCTACAATTAGCGGGTTAGATGACCCGATTGTGAACCCGGAATATGACCCGAAATTGGGACAGCTGGCGGCGGAGAGAGTGGTGGTTTATGTGGCGGAGAAGGATGCTCTGAAGAACAGAGGATGGTTTTACAGCGAGTGTTTGAAGAAATCGGGATGGGGTGGCACCGTCGAGGTCGTGGAGACAAAGGGCGAAGGCCACGTGTTTCATTTGTTCAACCCCACTACCGACGTGGCACTGGATTTCGTCAGAAAGCTTGCTGCCTTCATCAATGGCAATGGCGGCCGCCGTGAGTGA

mRNA sequence

ATGGATTCCACCGAAATTGCCCACAACTTTTCCCCTTTGTTTATCGTTTACAAAGACGGTACCGTCCACAGGTTACTCGGCAACGATGCAATACCTCCTTCTCCGGATCCCGTTACCGGCGTCGATTCCAAAGACGTCGTCATCTCCCCGGAAACCGCTGTCTCTGTTCGGATTTACAGGCCCAAACCCGCCACCGCCACCGCACAACCCCAGAAGCTTCCTGTTCTCGTTTACGTCCACGGCGGTGGCTTCTGCATCGAATCGGCATTCTCCCCTTTCTACGACCGCCACGTCAAGTCCTTAGTTGCTGAAGCCAACGTTGTCGCCGTCTCTGTCGACTACCGTCTTGCTCCGGAGCACCCTCTTCCGATTGCTTATGAAGATTCATGGGCCGCTCTGAAATGGGTCGCTGCCCATTCCGACGGAAATGGGCCGGAGGAGTGGCTGAATGGAATCGTGGATTTGGATCGGGTTTATTTTGCTGGAGATAGCGCCGGAGCTAACATTGTTCATCACTTGGCGGTTCGGGTCGGGTCGGAGGGGATAGGCGGGTTGAATCTGAAGGGATTGATTTTGGTTCATCCTTATTTCTGGGGAGGGAAATTGGTCGGAGATGAAGAGAAGCTGAGAGCAGAGGAAAGACACTTTATGGAGAAATTGTGGTATGTGACTTGCCCTACAATTAGCGGGTTAGATGACCCGATTGTGAACCCGGAATATGACCCGAAATTGGGACAGCTGGCGGCGGAGAGAGTGGTGGTTTATGTGGCGGAGAAGGATGCTCTGAAGAACAGAGGATGGTTTTACAGCGAGTGTTTGAAGAAATCGGGATGGGGTGGCACCGTCGAGGTCGTGGAGACAAAGGGCGAAGGCCACGTGTTTCATTTGTTCAACCCCACTACCGACGTGGCACTGGATTTCGTCAGAAAGCTTGCTGCCTTCATCAATGGCAATGGCGGCCGCCGTGAGTGA

Coding sequence (CDS)

ATGGATTCCACCGAAATTGCCCACAACTTTTCCCCTTTGTTTATCGTTTACAAAGACGGTACCGTCCACAGGTTACTCGGCAACGATGCAATACCTCCTTCTCCGGATCCCGTTACCGGCGTCGATTCCAAAGACGTCGTCATCTCCCCGGAAACCGCTGTCTCTGTTCGGATTTACAGGCCCAAACCCGCCACCGCCACCGCACAACCCCAGAAGCTTCCTGTTCTCGTTTACGTCCACGGCGGTGGCTTCTGCATCGAATCGGCATTCTCCCCTTTCTACGACCGCCACGTCAAGTCCTTAGTTGCTGAAGCCAACGTTGTCGCCGTCTCTGTCGACTACCGTCTTGCTCCGGAGCACCCTCTTCCGATTGCTTATGAAGATTCATGGGCCGCTCTGAAATGGGTCGCTGCCCATTCCGACGGAAATGGGCCGGAGGAGTGGCTGAATGGAATCGTGGATTTGGATCGGGTTTATTTTGCTGGAGATAGCGCCGGAGCTAACATTGTTCATCACTTGGCGGTTCGGGTCGGGTCGGAGGGGATAGGCGGGTTGAATCTGAAGGGATTGATTTTGGTTCATCCTTATTTCTGGGGAGGGAAATTGGTCGGAGATGAAGAGAAGCTGAGAGCAGAGGAAAGACACTTTATGGAGAAATTGTGGTATGTGACTTGCCCTACAATTAGCGGGTTAGATGACCCGATTGTGAACCCGGAATATGACCCGAAATTGGGACAGCTGGCGGCGGAGAGAGTGGTGGTTTATGTGGCGGAGAAGGATGCTCTGAAGAACAGAGGATGGTTTTACAGCGAGTGTTTGAAGAAATCGGGATGGGGTGGCACCGTCGAGGTCGTGGAGACAAAGGGCGAAGGCCACGTGTTTCATTTGTTCAACCCCACTACCGACGTGGCACTGGATTTCGTCAGAAAGCTTGCTGCCTTCATCAATGGCAATGGCGGCCGCCGTGAGTGA

Protein sequence

MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYRPKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLAPEHPLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEGIGGLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLWYVTCPTISGLDDPIVNPEYDPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPTTDVALDFVRKLAAFINGNGGRRE
Homology
BLAST of Cp4.1LG16g02150 vs. ExPASy Swiss-Prot
Match: Q9SMM9 (Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 7.9e-84
Identity = 161/330 (48.79%), Postives = 215/330 (65.15%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDS EIA ++SP+ I+YK G + RL+G   +PPS +P  GV SKDVV SP+  +S+RIY 
Sbjct: 1   MDS-EIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYL 60

Query: 61  PKPATATAQPQ---KLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLA 120
           P+ A ATA+ +   KLP+LVY HGGGF +E+AFSP Y   + + V+ ++ VAVSVDYR A
Sbjct: 61  PEKA-ATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRA 120

Query: 121 PEHPLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAV-- 180
           PEHP+P +Y+DSW ALKWV +H  G+G E+WLN   D  +V+ AGDSAGANI HH+ +  
Sbjct: 121 PEHPIPTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKA 180

Query: 181 ---RVGSEGIGGLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLWYVTCP-TISGL 240
              ++  E +    + G+ILVHPYFW    V D+E      R ++E +W +  P +  G 
Sbjct: 181 AKDKLSPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGS 240

Query: 241 DDPIVN--PEYDPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTV-EVVETK 300
           DDP +N        L  L   +V+V VAEKDAL  +GW Y E L KS W G V +VVETK
Sbjct: 241 DDPFINVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETK 300

Query: 301 GEGHVFHLFNPTTDVALDFVRKLAAFINGN 319
           GEGHVFHL +P ++ A + V + A FI G+
Sbjct: 301 GEGHVFHLRDPNSEKAHELVHRFAGFIKGD 328

BLAST of Cp4.1LG16g02150 vs. ExPASy Swiss-Prot
Match: Q9SMN0 (Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 3.9e-83
Identity = 157/324 (48.46%), Postives = 206/324 (63.58%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDS EIA + SPL  +YK G + RL+G   +PPS +P  GV SKDVV S +  +SVRIY 
Sbjct: 1   MDS-EIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYL 60

Query: 61  PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLAPEH 120
           P+ A A     KLP+LVY HGGGF IE+AFSP Y   + + V+ +N VAVSVDYR APEH
Sbjct: 61  PEKAAAETD-SKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEH 120

Query: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180
           P+ + ++DSW ALKWV  H  G+G E+WLN   D  RV+ +GDSAGANIVHH+A+R   E
Sbjct: 121 PISVPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKE 180

Query: 181 ----GIGGLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLWYVTCP-TISGLDDPI 240
               G+    + G+IL+HPYFW    + +++      R  +E  W +  P +  G DDP+
Sbjct: 181 KLSPGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPL 240

Query: 241 VN--PEYDPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHV 300
           +N        L  L   +V+V VAEKDAL  +GW Y+  L+KSGW G VEVVE++GE HV
Sbjct: 241 LNVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHV 300

Query: 301 FHLFNPTTDVALDFVRKLAAFING 318
           FHL  P  D A++ + K + FI G
Sbjct: 301 FHLLKPECDNAIEVMHKFSGFIKG 322

BLAST of Cp4.1LG16g02150 vs. ExPASy Swiss-Prot
Match: Q9ZQ91 (Probable carboxylesterase 7 OS=Arabidopsis thaliana OX=3702 GN=CXE7 PE=2 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 3.7e-81
Identity = 158/317 (49.84%), Postives = 198/317 (62.46%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDS  IA + SP+F VYK G + RLLG   +PPS  P  GV SKD++ SPE  +S+RIY 
Sbjct: 1   MDSV-IAFDRSPMFRVYKSGRIERLLGETTVPPSLTPQNGVVSKDIIHSPEKNLSLRIYL 60

Query: 61  PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLAPEH 120
           P+  T     +KLP+L+Y HGGGF IE+AFSP Y   + S VA AN +A+SV+YR APE 
Sbjct: 61  PEKVTV----KKLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEF 120

Query: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180
           P+PI YEDSW +LKWV  H  G GPE W+N   D  +V+ AGDSAG NI HHL +R   E
Sbjct: 121 PVPIPYEDSWDSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKE 180

Query: 181 GIGGLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLWYVTCP-TISGLDDPIVNPE 240
            +    + G+IL+HPYFW    + + E     +   +E  W V  P +  G+DDP +N  
Sbjct: 181 KLCDSLISGIILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVV 240

Query: 241 YDPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNP 300
                G L   RV+V VA  D    +GW Y+E LKKSGW G VEV+ETK EGHVFHL NP
Sbjct: 241 GSDPSG-LGCGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNP 300

Query: 301 TTDVALDFVRKLAAFIN 317
            +D A   V+KL  FIN
Sbjct: 301 NSDNARQVVKKLEEFIN 311

BLAST of Cp4.1LG16g02150 vs. ExPASy Swiss-Prot
Match: Q9SX78 (Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 8.2e-81
Identity = 157/321 (48.91%), Postives = 204/321 (63.55%), Query Frame = 0

Query: 1   MDST--EIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRI 60
           M+ST  +++    P  +V+ DGTV RL G +  PP  DP+TGV SKD++I P+T +S RI
Sbjct: 1   MESTKKQVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARI 60

Query: 61  YRPKPATATAQP-QKLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLA 120
           YRP     + QP QK+P+++Y HGG F I S   P Y   +  +V +ANV+AVSV+YRLA
Sbjct: 61  YRP----FSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLA 120

Query: 121 PEHPLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRV 180
           PEHPLP AYEDSW ALK + A +     E W+N   DLD ++  GDSAGANI HHLA R 
Sbjct: 121 PEHPLPTAYEDSWTALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFR- 180

Query: 181 GSEGIGGLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLWYVTCPTISGLDDPIVN 240
             +    L +KG+ ++HPYFWG + +G E K  A  +  ++  W   CP+  G DDP +N
Sbjct: 181 AKQSDQTLKIKGIGMIHPYFWGTQPIGAEIKDEA-RKQMVDGWWEFVCPSEKGSDDPWIN 240

Query: 241 PEYD--PKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFH 300
           P  D  P LG L  ERV++ VAEKD L  RG  Y E L KS W G VE++ETK + HVFH
Sbjct: 241 PFADGSPDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFH 300

Query: 301 LFNPTTDVALDFVRKLAAFIN 317
           +F P  D A++ VR LA FIN
Sbjct: 301 IFEPDCDEAMEMVRCLALFIN 310

BLAST of Cp4.1LG16g02150 vs. ExPASy Swiss-Prot
Match: Q9FX94 (Probable carboxylesterase 5 OS=Arabidopsis thaliana OX=3702 GN=CXE5 PE=2 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 2.2e-78
Identity = 145/317 (45.74%), Postives = 198/317 (62.46%), Query Frame = 0

Query: 4   TEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYRPKP 63
           +EIA  F P   +YKDG V RL+G D IP S DP   V SKDV+ SPE  +SVR++ P  
Sbjct: 3   SEIASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPHK 62

Query: 64  ATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLAPEHPLP 123
           +T      KLP+L+Y+HGG + IES FSP Y  ++  +V  AN +AVSV YR APE P+P
Sbjct: 63  STKLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPVP 122

Query: 124 IAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEGIG 183
            AYED W+A++W+ AHS+G+GP +W+N   D  +V+  GDSAG NI HH+A++ G E   
Sbjct: 123 AAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKKL 182

Query: 184 GLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLW-YVTCP-TISGLDDPIVNPE-Y 243
            L +KG+ +VHP FWG   V + +    E R  + ++W  +  P +++G DDP+ N    
Sbjct: 183 DLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNVNGS 242

Query: 244 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 303
                 L  ++V+V VA KD    +G  Y+  L+K  W GTVEVVE +GE HVFHL NP 
Sbjct: 243 GSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNPK 302

Query: 304 TDVALDFVRKLAAFING 318
           +D AL F++K   FI G
Sbjct: 303 SDKALKFLKKFVEFIIG 319

BLAST of Cp4.1LG16g02150 vs. NCBI nr
Match: XP_023512696.1 (probable carboxylesterase 12 [Cucurbita pepo subsp. pepo] >XP_023522494.1 probable carboxylesterase 12 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 670 bits (1729), Expect = 2.32e-243
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR
Sbjct: 1   MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60

Query: 61  PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLAPEH 120
           PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLAPEH
Sbjct: 61  PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLAPEH 120

Query: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180
           PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE
Sbjct: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180

Query: 181 GIGGLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLWYVTCPTISGLDDPIVNPEY 240
           GIGGLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLWYVTCPTISGLDDPIVNPEY
Sbjct: 181 GIGGLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLWYVTCPTISGLDDPIVNPEY 240

Query: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300
           DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT
Sbjct: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300

Query: 301 TDVALDFVRKLAAFINGNGGRRE 323
           TDVALDFVRKLAAFINGNGGRRE
Sbjct: 301 TDVALDFVRKLAAFINGNGGRRE 323

BLAST of Cp4.1LG16g02150 vs. NCBI nr
Match: XP_022944692.1 (probable carboxylesterase 13 [Cucurbita moschata])

HSP 1 Score: 653 bits (1684), Expect = 1.57e-236
Identity = 316/323 (97.83%), Postives = 317/323 (98.14%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDSTEIAHNFSPLFIVYKDGTVHRL+GNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR
Sbjct: 1   MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60

Query: 61  PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLAPEH 120
           PKPATA  QPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSL AEANVVAVSVDYRLAPEH
Sbjct: 61  PKPATA--QPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEH 120

Query: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180
           PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE
Sbjct: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180

Query: 181 GIGGLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLWYVTCPTISGLDDPIVNPEY 240
           GI GLNLKGLILVHPYFWGGKLVGDEEKLR EERHFMEKLWYVTCPTISGLDDPIVNPEY
Sbjct: 181 GIAGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPEY 240

Query: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300
           DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT
Sbjct: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300

Query: 301 TDVALDFVRKLAAFINGNGGRRE 323
           TDVALDFVR LAAFINGNGGRRE
Sbjct: 301 TDVALDFVRNLAAFINGNGGRRE 321

BLAST of Cp4.1LG16g02150 vs. NCBI nr
Match: XP_022986571.1 (probable carboxylesterase 12 [Cucurbita maxima])

HSP 1 Score: 651 bits (1680), Expect = 6.87e-236
Identity = 313/323 (96.90%), Postives = 316/323 (97.83%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDSTEIAHNFSPLFIVYKDGTVHR LGND IPPSPDP+TGVDSKDVVISPETAVSVRIYR
Sbjct: 1   MDSTEIAHNFSPLFIVYKDGTVHRFLGNDVIPPSPDPLTGVDSKDVVISPETAVSVRIYR 60

Query: 61  PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLAPEH 120
           PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSL AEANVVAVSVDYRLAPEH
Sbjct: 61  PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLSAEANVVAVSVDYRLAPEH 120

Query: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180
           PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE
Sbjct: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180

Query: 181 GIGGLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLWYVTCPTISGLDDPIVNPEY 240
           GI GLNLKGLILVHPYFWGGKLVGDEEKLR EERHFMEKLWYVTCPTISGLDD IVNPEY
Sbjct: 181 GIPGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDSIVNPEY 240

Query: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300
           DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT
Sbjct: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300

Query: 301 TDVALDFVRKLAAFINGNGGRRE 323
           TDVA+DFVRKLAAFINGNGGR +
Sbjct: 301 TDVAVDFVRKLAAFINGNGGRHD 323

BLAST of Cp4.1LG16g02150 vs. NCBI nr
Match: KAG6571159.1 (putative carboxylesterase 13, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 647 bits (1670), Expect = 2.67e-234
Identity = 317/329 (96.35%), Postives = 317/329 (96.35%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR
Sbjct: 1   MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60

Query: 61  PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLAPEH 120
           PKPATA  QPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSL AEANVVAVSVDYRLAPEH
Sbjct: 61  PKPATA--QPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEH 120

Query: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180
           PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE
Sbjct: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180

Query: 181 GIGGLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLWYVTCPTISGLDDPIVNPEY 240
           GI GLNLKGLILVHPYFWGGKLVGDEEKLR EERHFMEKLWYVTCPTISGLDDPIVNPEY
Sbjct: 181 GIAGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPEY 240

Query: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300
           DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT
Sbjct: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300

Query: 301 TDVALDFVRKLAAFINGNG------GRRE 323
           TDVALDFVR LAAFINGNG      GRRE
Sbjct: 301 TDVALDFVRNLAAFINGNGNGNGNGGRRE 327

BLAST of Cp4.1LG16g02150 vs. NCBI nr
Match: XP_038902770.1 (probable carboxylesterase 13 [Benincasa hispida])

HSP 1 Score: 535 bits (1378), Expect = 6.02e-190
Identity = 254/323 (78.64%), Postives = 283/323 (87.62%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           M STEIAH+FSP+ +VYKDG +HRL GN+ I PS DP+TGV+SKDVVISPET VS RIY+
Sbjct: 1   MASTEIAHDFSPMLVVYKDGRIHRLAGNELIDPSLDPLTGVESKDVVISPETPVSARIYK 60

Query: 61  PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLAPEH 120
           PKP   TAQP KLP+LVY HGGGFCIESAFS FY RH+ SLVAEANVVAVSVDYRLAPEH
Sbjct: 61  PKP---TAQPHKLPLLVYFHGGGFCIESAFSSFYHRHLNSLVAEANVVAVSVDYRLAPEH 120

Query: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180
           PLPIAYEDSW ALKWVAAHS G GPEEWLNGI DLDRVYFAGDSAGANI + +A+RVG+E
Sbjct: 121 PLPIAYEDSWDALKWVAAHSAGTGPEEWLNGIADLDRVYFAGDSAGANIANRMAIRVGTE 180

Query: 181 GIGGLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLWYVTCPTISGLDDPIVNPEY 240
           G+ GLNL+GL+LVH YFWGGKL+GDEEKL  E R F+EKLWY  CP ISGLDDPIVNP +
Sbjct: 181 GLAGLNLRGLLLVHAYFWGGKLIGDEEKLNPEHRSFIEKLWYFVCPKISGLDDPIVNPGF 240

Query: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300
           DP+LG++AAERV V+VAEKDALKNRGWFYSECLKKSGWGG V+V+ETKG+GHVFHLFNPT
Sbjct: 241 DPELGRVAAERVAVFVAEKDALKNRGWFYSECLKKSGWGGAVDVIETKGQGHVFHLFNPT 300

Query: 301 TDVALDFVRKLAAFINGNGGRRE 323
           +D A+DFV KLAAFING   RRE
Sbjct: 301 SDEAVDFVGKLAAFINGE--RRE 318

BLAST of Cp4.1LG16g02150 vs. ExPASy TrEMBL
Match: A0A6J1FW98 (probable carboxylesterase 13 OS=Cucurbita moschata OX=3662 GN=LOC111449075 PE=4 SV=1)

HSP 1 Score: 653 bits (1684), Expect = 7.58e-237
Identity = 316/323 (97.83%), Postives = 317/323 (98.14%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDSTEIAHNFSPLFIVYKDGTVHRL+GNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR
Sbjct: 1   MDSTEIAHNFSPLFIVYKDGTVHRLVGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60

Query: 61  PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLAPEH 120
           PKPATA  QPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSL AEANVVAVSVDYRLAPEH
Sbjct: 61  PKPATA--QPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLAAEANVVAVSVDYRLAPEH 120

Query: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180
           PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE
Sbjct: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180

Query: 181 GIGGLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLWYVTCPTISGLDDPIVNPEY 240
           GI GLNLKGLILVHPYFWGGKLVGDEEKLR EERHFMEKLWYVTCPTISGLDDPIVNPEY
Sbjct: 181 GIAGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDPIVNPEY 240

Query: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300
           DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT
Sbjct: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300

Query: 301 TDVALDFVRKLAAFINGNGGRRE 323
           TDVALDFVR LAAFINGNGGRRE
Sbjct: 301 TDVALDFVRNLAAFINGNGGRRE 321

BLAST of Cp4.1LG16g02150 vs. ExPASy TrEMBL
Match: A0A6J1JBI9 (probable carboxylesterase 12 OS=Cucurbita maxima OX=3661 GN=LOC111484271 PE=4 SV=1)

HSP 1 Score: 651 bits (1680), Expect = 3.33e-236
Identity = 313/323 (96.90%), Postives = 316/323 (97.83%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDSTEIAHNFSPLFIVYKDGTVHR LGND IPPSPDP+TGVDSKDVVISPETAVSVRIYR
Sbjct: 1   MDSTEIAHNFSPLFIVYKDGTVHRFLGNDVIPPSPDPLTGVDSKDVVISPETAVSVRIYR 60

Query: 61  PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLAPEH 120
           PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSL AEANVVAVSVDYRLAPEH
Sbjct: 61  PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLSAEANVVAVSVDYRLAPEH 120

Query: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180
           PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE
Sbjct: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180

Query: 181 GIGGLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLWYVTCPTISGLDDPIVNPEY 240
           GI GLNLKGLILVHPYFWGGKLVGDEEKLR EERHFMEKLWYVTCPTISGLDD IVNPEY
Sbjct: 181 GIPGLNLKGLILVHPYFWGGKLVGDEEKLRPEERHFMEKLWYVTCPTISGLDDSIVNPEY 240

Query: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300
           DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT
Sbjct: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300

Query: 301 TDVALDFVRKLAAFINGNGGRRE 323
           TDVA+DFVRKLAAFINGNGGR +
Sbjct: 301 TDVAVDFVRKLAAFINGNGGRHD 323

BLAST of Cp4.1LG16g02150 vs. ExPASy TrEMBL
Match: A0A5A7SPY3 (Putative carboxylesterase 13 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G00320 PE=4 SV=1)

HSP 1 Score: 522 bits (1344), Expect = 4.40e-185
Identity = 243/317 (76.66%), Postives = 278/317 (87.70%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           M STEIAHNFSP+ IVYKDG   RL+GN+ I PS DP+TGV+SKD++ISPET VS RIYR
Sbjct: 1   MASTEIAHNFSPMIIVYKDGRTERLVGNELIDPSLDPLTGVESKDIIISPETPVSARIYR 60

Query: 61  PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLAPEH 120
           PKP   TA+P KLP+L+Y HGGGFCIESAFSP Y  H+ SLVAEANV+A+SV+YR APE+
Sbjct: 61  PKP---TAEPHKLPLLIYFHGGGFCIESAFSPTYHNHLNSLVAEANVIAISVEYRRAPEY 120

Query: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180
           PLPIAYED W ALKWVAAHS G GPEEWLN I DL+RVYFAGDSAGANI + +A+RVG E
Sbjct: 121 PLPIAYEDCWTALKWVAAHSAGTGPEEWLNEIADLNRVYFAGDSAGANIANKMAIRVGIE 180

Query: 181 GIGGLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLWYVTCPTISGLDDPIVNPEY 240
           G+ GLNLKGL+L+HPYFWG KL+GDEEKL+ E+R F+EKLWY+ CPTISGLDDP+VNPE+
Sbjct: 181 GVAGLNLKGLMLIHPYFWGEKLIGDEEKLKPEDRWFIEKLWYLACPTISGLDDPVVNPEF 240

Query: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300
           +PKLG LAAERV VYVAEKD+LK+RGWFYSECLKKSGWGG VEVVETKG+GHVFHLFNPT
Sbjct: 241 EPKLGGLAAERVAVYVAEKDSLKDRGWFYSECLKKSGWGGAVEVVETKGQGHVFHLFNPT 300

Query: 301 TDVALDFVRKLAAFING 317
           +D A+DFV KLAAFING
Sbjct: 301 SDDAVDFVGKLAAFING 314

BLAST of Cp4.1LG16g02150 vs. ExPASy TrEMBL
Match: A0A1S3CAV3 (probable carboxylesterase 13 OS=Cucumis melo OX=3656 GN=LOC103498400 PE=4 SV=1)

HSP 1 Score: 522 bits (1344), Expect = 4.40e-185
Identity = 243/317 (76.66%), Postives = 278/317 (87.70%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           M STEIAHNFSP+ IVYKDG   RL+GN+ I PS DP+TGV+SKD++ISPET VS RIYR
Sbjct: 1   MASTEIAHNFSPMIIVYKDGRTERLVGNELIDPSLDPLTGVESKDIIISPETPVSARIYR 60

Query: 61  PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLAPEH 120
           PKP   TA+P KLP+L+Y HGGGFCIESAFSP Y  H+ SLVAEANV+A+SV+YR APE+
Sbjct: 61  PKP---TAEPHKLPLLIYFHGGGFCIESAFSPTYHNHLNSLVAEANVIAISVEYRRAPEY 120

Query: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180
           PLPIAYED W ALKWVAAHS G GPEEWLN I DL+RVYFAGDSAGANI + +A+RVG E
Sbjct: 121 PLPIAYEDCWTALKWVAAHSAGTGPEEWLNEIADLNRVYFAGDSAGANIANKMAIRVGIE 180

Query: 181 GIGGLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLWYVTCPTISGLDDPIVNPEY 240
           G+ GLNLKGL+L+HPYFWG KL+GDEEKL+ E+R F+EKLWY+ CPTISGLDDP+VNPE+
Sbjct: 181 GVAGLNLKGLMLIHPYFWGEKLIGDEEKLKPEDRWFIEKLWYLACPTISGLDDPVVNPEF 240

Query: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300
           +PKLG LAAERV VYVAEKD+LK+RGWFYSECLKKSGWGG VEVVETKG+GHVFHLFNPT
Sbjct: 241 EPKLGGLAAERVAVYVAEKDSLKDRGWFYSECLKKSGWGGAVEVVETKGQGHVFHLFNPT 300

Query: 301 TDVALDFVRKLAAFING 317
           +D A+DFV KLAAFING
Sbjct: 301 SDDAVDFVGKLAAFING 314

BLAST of Cp4.1LG16g02150 vs. ExPASy TrEMBL
Match: A0A0A0LIH8 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G234520 PE=4 SV=1)

HSP 1 Score: 508 bits (1309), Expect = 9.40e-180
Identity = 238/317 (75.08%), Postives = 273/317 (86.12%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           M STEIA+N SP+ IVYKDG   RL+GN+ + PS DP+T V+SKD+VISPET VS RIYR
Sbjct: 1   MASTEIAYNLSPMLIVYKDGRAERLVGNELVHPSLDPLTVVESKDIVISPETPVSARIYR 60

Query: 61  PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLAPEH 120
           PKP   TA+P KLP+L+Y+HGGGFCIESAFSP Y  H+ SLVAEANV+A+SV+YR APEH
Sbjct: 61  PKP---TAEPHKLPLLIYIHGGGFCIESAFSPTYHHHLNSLVAEANVIAISVEYRRAPEH 120

Query: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180
           PLPIAYEDSW ALKWVAAHS G GPEEWLN I D +RVYFAGDSAGAN+ + +A+RVG E
Sbjct: 121 PLPIAYEDSWTALKWVAAHSAGTGPEEWLNKIADFNRVYFAGDSAGANVANKMAIRVGME 180

Query: 181 GIGGLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLWYVTCPTISGLDDPIVNPEY 240
           G+ GLNLKGL+LVHPYFWG KL+GDEEKL+ EER F+EKLWYV CPTISGLDDPIVNPE+
Sbjct: 181 GVAGLNLKGLMLVHPYFWGEKLIGDEEKLKPEERWFIEKLWYVACPTISGLDDPIVNPEF 240

Query: 241 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 300
           +P LG++ AERV VYVAEKDALK+RG FYSECLKKSGWGG VEV ETKG+GHVFHLFNPT
Sbjct: 241 EPNLGKVTAERVAVYVAEKDALKDRGRFYSECLKKSGWGGAVEVTETKGQGHVFHLFNPT 300

Query: 301 TDVALDFVRKLAAFING 317
           +D A+ FV KLAAF+NG
Sbjct: 301 SDDAVQFVGKLAAFLNG 314

BLAST of Cp4.1LG16g02150 vs. TAIR 10
Match: AT3G48700.1 (carboxyesterase 13 )

HSP 1 Score: 312.0 bits (798), Expect = 5.6e-85
Identity = 161/330 (48.79%), Postives = 215/330 (65.15%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDS EIA ++SP+ I+YK G + RL+G   +PPS +P  GV SKDVV SP+  +S+RIY 
Sbjct: 1   MDS-EIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYL 60

Query: 61  PKPATATAQPQ---KLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLA 120
           P+ A ATA+ +   KLP+LVY HGGGF +E+AFSP Y   + + V+ ++ VAVSVDYR A
Sbjct: 61  PEKA-ATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRA 120

Query: 121 PEHPLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAV-- 180
           PEHP+P +Y+DSW ALKWV +H  G+G E+WLN   D  +V+ AGDSAGANI HH+ +  
Sbjct: 121 PEHPIPTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKA 180

Query: 181 ---RVGSEGIGGLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLWYVTCP-TISGL 240
              ++  E +    + G+ILVHPYFW    V D+E      R ++E +W +  P +  G 
Sbjct: 181 AKDKLSPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGS 240

Query: 241 DDPIVN--PEYDPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTV-EVVETK 300
           DDP +N        L  L   +V+V VAEKDAL  +GW Y E L KS W G V +VVETK
Sbjct: 241 DDPFINVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETK 300

Query: 301 GEGHVFHLFNPTTDVALDFVRKLAAFINGN 319
           GEGHVFHL +P ++ A + V + A FI G+
Sbjct: 301 GEGHVFHLRDPNSEKAHELVHRFAGFIKGD 328

BLAST of Cp4.1LG16g02150 vs. TAIR 10
Match: AT3G48690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 309.7 bits (792), Expect = 2.8e-84
Identity = 157/324 (48.46%), Postives = 206/324 (63.58%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDS EIA + SPL  +YK G + RL+G   +PPS +P  GV SKDVV S +  +SVRIY 
Sbjct: 1   MDS-EIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYL 60

Query: 61  PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLAPEH 120
           P+ A A     KLP+LVY HGGGF IE+AFSP Y   + + V+ +N VAVSVDYR APEH
Sbjct: 61  PEKAAAETD-SKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEH 120

Query: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180
           P+ + ++DSW ALKWV  H  G+G E+WLN   D  RV+ +GDSAGANIVHH+A+R   E
Sbjct: 121 PISVPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKE 180

Query: 181 ----GIGGLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLWYVTCP-TISGLDDPI 240
               G+    + G+IL+HPYFW    + +++      R  +E  W +  P +  G DDP+
Sbjct: 181 KLSPGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPL 240

Query: 241 VN--PEYDPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHV 300
           +N        L  L   +V+V VAEKDAL  +GW Y+  L+KSGW G VEVVE++GE HV
Sbjct: 241 LNVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHV 300

Query: 301 FHLFNPTTDVALDFVRKLAAFING 318
           FHL  P  D A++ + K + FI G
Sbjct: 301 FHLLKPECDNAIEVMHKFSGFIKG 322

BLAST of Cp4.1LG16g02150 vs. TAIR 10
Match: AT2G03550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 303.1 bits (775), Expect = 2.6e-82
Identity = 158/317 (49.84%), Postives = 198/317 (62.46%), Query Frame = 0

Query: 1   MDSTEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYR 60
           MDS  IA + SP+F VYK G + RLLG   +PPS  P  GV SKD++ SPE  +S+RIY 
Sbjct: 1   MDSV-IAFDRSPMFRVYKSGRIERLLGETTVPPSLTPQNGVVSKDIIHSPEKNLSLRIYL 60

Query: 61  PKPATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLAPEH 120
           P+  T     +KLP+L+Y HGGGF IE+AFSP Y   + S VA AN +A+SV+YR APE 
Sbjct: 61  PEKVTV----KKLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEF 120

Query: 121 PLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSE 180
           P+PI YEDSW +LKWV  H  G GPE W+N   D  +V+ AGDSAG NI HHL +R   E
Sbjct: 121 PVPIPYEDSWDSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKE 180

Query: 181 GIGGLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLWYVTCP-TISGLDDPIVNPE 240
            +    + G+IL+HPYFW    + + E     +   +E  W V  P +  G+DDP +N  
Sbjct: 181 KLCDSLISGIILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVV 240

Query: 241 YDPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNP 300
                G L   RV+V VA  D    +GW Y+E LKKSGW G VEV+ETK EGHVFHL NP
Sbjct: 241 GSDPSG-LGCGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNP 300

Query: 301 TTDVALDFVRKLAAFIN 317
            +D A   V+KL  FIN
Sbjct: 301 NSDNARQVVKKLEEFIN 311

BLAST of Cp4.1LG16g02150 vs. TAIR 10
Match: AT1G47480.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 302.0 bits (772), Expect = 5.8e-82
Identity = 157/321 (48.91%), Postives = 204/321 (63.55%), Query Frame = 0

Query: 1   MDST--EIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRI 60
           M+ST  +++    P  +V+ DGTV RL G +  PP  DP+TGV SKD++I P+T +S RI
Sbjct: 1   MESTKKQVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARI 60

Query: 61  YRPKPATATAQP-QKLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLA 120
           YRP     + QP QK+P+++Y HGG F I S   P Y   +  +V +ANV+AVSV+YRLA
Sbjct: 61  YRP----FSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLA 120

Query: 121 PEHPLPIAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRV 180
           PEHPLP AYEDSW ALK + A +     E W+N   DLD ++  GDSAGANI HHLA R 
Sbjct: 121 PEHPLPTAYEDSWTALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFR- 180

Query: 181 GSEGIGGLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLWYVTCPTISGLDDPIVN 240
             +    L +KG+ ++HPYFWG + +G E K  A  +  ++  W   CP+  G DDP +N
Sbjct: 181 AKQSDQTLKIKGIGMIHPYFWGTQPIGAEIKDEA-RKQMVDGWWEFVCPSEKGSDDPWIN 240

Query: 241 PEYD--PKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFH 300
           P  D  P LG L  ERV++ VAEKD L  RG  Y E L KS W G VE++ETK + HVFH
Sbjct: 241 PFADGSPDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFH 300

Query: 301 LFNPTTDVALDFVRKLAAFIN 317
           +F P  D A++ VR LA FIN
Sbjct: 301 IFEPDCDEAMEMVRCLALFIN 310

BLAST of Cp4.1LG16g02150 vs. TAIR 10
Match: AT1G49660.1 (carboxyesterase 5 )

HSP 1 Score: 293.9 bits (751), Expect = 1.6e-79
Identity = 145/317 (45.74%), Postives = 198/317 (62.46%), Query Frame = 0

Query: 4   TEIAHNFSPLFIVYKDGTVHRLLGNDAIPPSPDPVTGVDSKDVVISPETAVSVRIYRPKP 63
           +EIA  F P   +YKDG V RL+G D IP S DP   V SKDV+ SPE  +SVR++ P  
Sbjct: 3   SEIASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPHK 62

Query: 64  ATATAQPQKLPVLVYVHGGGFCIESAFSPFYDRHVKSLVAEANVVAVSVDYRLAPEHPLP 123
           +T      KLP+L+Y+HGG + IES FSP Y  ++  +V  AN +AVSV YR APE P+P
Sbjct: 63  STKLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPVP 122

Query: 124 IAYEDSWAALKWVAAHSDGNGPEEWLNGIVDLDRVYFAGDSAGANIVHHLAVRVGSEGIG 183
            AYED W+A++W+ AHS+G+GP +W+N   D  +V+  GDSAG NI HH+A++ G E   
Sbjct: 123 AAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKKL 182

Query: 184 GLNLKGLILVHPYFWGGKLVGDEEKLRAEERHFMEKLW-YVTCP-TISGLDDPIVNPE-Y 243
            L +KG+ +VHP FWG   V + +    E R  + ++W  +  P +++G DDP+ N    
Sbjct: 183 DLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNVNGS 242

Query: 244 DPKLGQLAAERVVVYVAEKDALKNRGWFYSECLKKSGWGGTVEVVETKGEGHVFHLFNPT 303
                 L  ++V+V VA KD    +G  Y+  L+K  W GTVEVVE +GE HVFHL NP 
Sbjct: 243 GSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNPK 302

Query: 304 TDVALDFVRKLAAFING 318
           +D AL F++K   FI G
Sbjct: 303 SDKALKFLKKFVEFIIG 319

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SMM97.9e-8448.79Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1[more]
Q9SMN03.9e-8348.46Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1[more]
Q9ZQ913.7e-8149.84Probable carboxylesterase 7 OS=Arabidopsis thaliana OX=3702 GN=CXE7 PE=2 SV=1[more]
Q9SX788.2e-8148.91Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1[more]
Q9FX942.2e-7845.74Probable carboxylesterase 5 OS=Arabidopsis thaliana OX=3702 GN=CXE5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023512696.12.32e-243100.00probable carboxylesterase 12 [Cucurbita pepo subsp. pepo] >XP_023522494.1 probab... [more]
XP_022944692.11.57e-23697.83probable carboxylesterase 13 [Cucurbita moschata][more]
XP_022986571.16.87e-23696.90probable carboxylesterase 12 [Cucurbita maxima][more]
KAG6571159.12.67e-23496.35putative carboxylesterase 13, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038902770.16.02e-19078.64probable carboxylesterase 13 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1FW987.58e-23797.83probable carboxylesterase 13 OS=Cucurbita moschata OX=3662 GN=LOC111449075 PE=4 ... [more]
A0A6J1JBI93.33e-23696.90probable carboxylesterase 12 OS=Cucurbita maxima OX=3661 GN=LOC111484271 PE=4 SV... [more]
A0A5A7SPY34.40e-18576.66Putative carboxylesterase 13 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3CAV34.40e-18576.66probable carboxylesterase 13 OS=Cucumis melo OX=3656 GN=LOC103498400 PE=4 SV=1[more]
A0A0A0LIH89.40e-18075.08Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G2345... [more]
Match NameE-valueIdentityDescription
AT3G48700.15.6e-8548.79carboxyesterase 13 [more]
AT3G48690.12.8e-8448.46alpha/beta-Hydrolases superfamily protein [more]
AT2G03550.12.6e-8249.84alpha/beta-Hydrolases superfamily protein [more]
AT1G47480.15.8e-8248.91alpha/beta-Hydrolases superfamily protein [more]
AT1G49660.11.6e-7945.74carboxyesterase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 76..295
e-value: 5.4E-50
score: 170.2
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 9..318
e-value: 2.9E-76
score: 259.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 11..317
NoneNo IPR availablePANTHERPTHR23024:SF458CARBOXYLESTERASE 2-RELATEDcoord: 3..316
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 3..316
IPR033140Lipase, GDXG, putative serine active sitePROSITEPS01174LIPASE_GDXG_SERcoord: 158..170

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG16g02150.1Cp4.1LG16g02150.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity