Cp4.1LG16g02120 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG16g02120
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationCp4.1LG16: 4245377 .. 4247378 (-)
RNA-Seq ExpressionCp4.1LG16g02120
SyntenyCp4.1LG16g02120
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTGTCCAACTTCGAACCGCCATTCATTTTCTGCTCATCAATGGCTTCTTCAACAAACGACGTCGCCGACGAGTTCTTTCCCTTCTTAAGAATCTACAAAGACGGCCGCATCCAACGGCTCCAAGTCACCTCCGACATAGTCCCCGCCTGCGCCGACGACCCCAAATCCGCTTTCCGAGCAAAGGATGTCACGATCTCCGTGGATTCCGCCCTTTCGGTGCGTATTTTCATCCCCTCCGCCGTAGATCTGAATCAGAAACTGCCCCTTCTGTTGTACGTCCACGGCGGAGCCTTCTGCTTCGAATCGGCTTTTAATTTGCAGTACCATAGCCATGTGGGTTCTCTCGCTGCTAAGGCCAACGCCGTCGCTGTTTCCGTCGAGTACCGCCTCGCGCCGGAGAATCCAATCCCCGCCTGCTATGACGATTGTTGGGATGCGTTACGGTGGATCGCGGCCCATGTGAACAGGTGAGGCTGATTTCAATTTTTTATTTTATTTTTTAATATCAAATATTCTTTTTAACTTATTAAAATATTTAGATATTTATAATTTCATTATCAATATATATAAAGATATTTAGATACTTTTAAATTTTTTAAAAAATATTTTAGAAAAAAAAAATATTTAGATATTTTAGAAATTATAGATTTAAACGGCAAAAATAGAGTGTCTTATAATCTTTTATTGATTGGTTCTAACACAAGTGTGAGTATTTGCCACAAAATAATTGTGTTCAACAATCTGGTATTAGAGTGAAGGTGGTGTAAGTTGTCGAATCTCGAATAATTCTTAATATGCTCGGTGGGAACCTACTCCAAATGGGTGAAAAAATTTGAGCTTAAACTGAGAATGAGAGACCGGACGAAGAATATATTCCTCGTCCGAGGCCAGGAACCCGGTCTAAATTCTTAAAAATTGAGAAAATTTATCTCTAGCATGATTTTATTTTAAAAGTCTTTCTCGTTAAAATATTATCACTAGCCAAGTAGTGTCAAGCCCAATAATCATCTAGCCGGGTAAATACCCTTCAAAGTTTTCATCCAAGAAATACAAAACAGTAATATTCTACATGAAAATGTTTGTTTAATCCACCACACTTAAATGATGTGAAAGTTATCTTATTTATAATCAAATAAACTACAATGATACGGGAAGAGTAATAAATAGAAAGAATAATTATAATAAAATATCAAATACGATAATTTAGTACTAAATATCTTAATCACTAGTCAGGAGATGTCTGGTAATATCTTTTTAATTCATTTTTAGTTTGTGTTCAAAACCTTGGGCGCCAACAGGGATGGTCCAGAGCCGTGGCTGAACCAGCACGCAGATTTGGACAGGATCTGTTTAGCTGGCGACAGTGCGGGCGCCAACATCTGCCATCACTTGGCTGTACGAGCAGGATCATCAGCAGAGCAGCTGGGCAGAGTAAAGGTGGTGGCATTGGCTTTGATTCACCCGTTCTTTGGCGACGGGGCGGGGGACCGGCTGTGGAAGTATCTGTGTTCGGACACCACTTGGCTGAGACCCACTGCACAGGATCTCGCCACGTTGCCTTGTCGGAGAGTGAAGATCTTTCTGGCAGAGAAAGATTCTCTGAAGTTCGGAGGGTGGAATTACGATGAGGATTTGAAGAGCAGCGGGTGGAACGGGGCGGTGGAGACGGTGGAGCACGGCGGAGAGGATCATGTGTTTCATTTAAAGATGCCGGAGTGTGAAAAGGCCGTTGATTTGATGGAAAAATTAGCCTGTTTCATAAACTTAGATTAGGATCAAATTAAGCTAATCTTAAAATAAATCTCTTAGCTTAAAGGTTCGGGTTTGTATTTGAATTAGACTTCTTCGACAATAATCGCACGACTTTTGAATGAATTGTAACACGAATTGATGACGGGTTTTTTTAGATGTGATTGGTCGAAAAAGTTCGAATAAAACTTTAGTTGTCTTTTTTATAATCGAATAAAACATAAGTGACTTTATGATTGGGTTAGATAATT

mRNA sequence

AATTGTCCAACTTCGAACCGCCATTCATTTTCTGCTCATCAATGGCTTCTTCAACAAACGACGTCGCCGACGAGTTCTTTCCCTTCTTAAGAATCTACAAAGACGGCCGCATCCAACGGCTCCAAGTCACCTCCGACATAGTCCCCGCCTGCGCCGACGACCCCAAATCCGCTTTCCGAGCAAAGGATGTCACGATCTCCGTGGATTCCGCCCTTTCGGTGCGTATTTTCATCCCCTCCGCCGTAGATCTGAATCAGAAACTGCCCCTTCTGTTGTACGTCCACGGCGGAGCCTTCTGCTTCGAATCGGCTTTTAATTTGCAGTACCATAGCCATGTGGGTTCTCTCGCTGCTAAGGCCAACGCCGTCGCTGTTTCCGTCGAGTACCGCCTCGCGCCGGAGAATCCAATCCCCGCCTGCTATGACGATTGTTGGGATGCGTTACGGTGGATCGCGGCCCATGTGAACAGGGATGGTCCAGAGCCGTGGCTGAACCAGCACGCAGATTTGGACAGGATCTGTTTAGCTGGCGACAGTGCGGGCGCCAACATCTGCCATCACTTGGCTGTACGAGCAGGATCATCAGCAGAGCAGCTGGGCAGAGTAAAGGTGGTGGCATTGGCTTTGATTCACCCGTTCTTTGGCGACGGGGCGGGGGACCGGCTGTGGAAGTATCTGTGTTCGGACACCACTTGGCTGAGACCCACTGCACAGGATCTCGCCACGTTGCCTTGTCGGAGAGTGAAGATCTTTCTGGCAGAGAAAGATTCTCTGAAGTTCGGAGGGTGGAATTACGATGAGGATTTGAAGAGCAGCGGGTGGAACGGGGCGGTGGAGACGGTGGAGCACGGCGGAGAGGATCATGTGTTTCATTTAAAGATGCCGGAGTGTGAAAAGGCCGTTGATTTGATGGAAAAATTAGCCTGTTTCATAAACTTAGATTAGGATCAAATTAAGCTAATCTTAAAATAAATCTCTTAGCTTAAAGGTTCGGGTTTGTATTTGAATTAGACTTCTTCGACAATAATCGCACGACTTTTGAATGAATTGTAACACGAATTGATGACGGGTTTTTTTAGATGTGATTGGTCGAAAAAGTTCGAATAAAACTTTAGTTGTCTTTTTTATAATCGAATAAAACATAAGTGACTTTATGATTGGGTTAGATAATT

Coding sequence (CDS)

ATGGCTTCTTCAACAAACGACGTCGCCGACGAGTTCTTTCCCTTCTTAAGAATCTACAAAGACGGCCGCATCCAACGGCTCCAAGTCACCTCCGACATAGTCCCCGCCTGCGCCGACGACCCCAAATCCGCTTTCCGAGCAAAGGATGTCACGATCTCCGTGGATTCCGCCCTTTCGGTGCGTATTTTCATCCCCTCCGCCGTAGATCTGAATCAGAAACTGCCCCTTCTGTTGTACGTCCACGGCGGAGCCTTCTGCTTCGAATCGGCTTTTAATTTGCAGTACCATAGCCATGTGGGTTCTCTCGCTGCTAAGGCCAACGCCGTCGCTGTTTCCGTCGAGTACCGCCTCGCGCCGGAGAATCCAATCCCCGCCTGCTATGACGATTGTTGGGATGCGTTACGGTGGATCGCGGCCCATGTGAACAGGGATGGTCCAGAGCCGTGGCTGAACCAGCACGCAGATTTGGACAGGATCTGTTTAGCTGGCGACAGTGCGGGCGCCAACATCTGCCATCACTTGGCTGTACGAGCAGGATCATCAGCAGAGCAGCTGGGCAGAGTAAAGGTGGTGGCATTGGCTTTGATTCACCCGTTCTTTGGCGACGGGGCGGGGGACCGGCTGTGGAAGTATCTGTGTTCGGACACCACTTGGCTGAGACCCACTGCACAGGATCTCGCCACGTTGCCTTGTCGGAGAGTGAAGATCTTTCTGGCAGAGAAAGATTCTCTGAAGTTCGGAGGGTGGAATTACGATGAGGATTTGAAGAGCAGCGGGTGGAACGGGGCGGTGGAGACGGTGGAGCACGGCGGAGAGGATCATGTGTTTCATTTAAAGATGCCGGAGTGTGAAAAGGCCGTTGATTTGATGGAAAAATTAGCCTGTTTCATAAACTTAGATTAG

Protein sequence

MASSTNDVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSALSVRIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPENPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGSSAEQLGRVKVVALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAEKDSLKFGGWNYDEDLKSSGWNGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFINLD
Homology
BLAST of Cp4.1LG16g02120 vs. ExPASy Swiss-Prot
Match: Q9SMN0 (Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 5.8e-65
Identity = 131/327 (40.06%), Postives = 188/327 (57.49%), Query Frame = 0

Query: 6   NDVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSALSVRIFIP 65
           +++A +  P L+IYK GRI+RL   + + P  + +P++   +KDV  S D+ LSVRI++P
Sbjct: 3   SEIAVDCSPLLKIYKSGRIERLMGEATVPP--SSEPQNGVVSKDVVYSADNNLSVRIYLP 62

Query: 66  --SAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPENPI 125
             +A + + KLPLL+Y HGG F  E+AF+  YH+ + +  + +N VAVSV+YR APE+PI
Sbjct: 63  EKAAAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPI 122

Query: 126 PACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGSS-- 185
              +DD W AL+W+  H+   G E WLN+HAD  R+ L+GDSAGANI HH+A+RA     
Sbjct: 123 SVPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKL 182

Query: 186 AEQLGRVKVVALALIHPFF-------------------------------GDGAGDRLWK 245
           +  L    +  + L+HP+F                                DG  D L  
Sbjct: 183 SPGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLN 242

Query: 246 YLCSDTTWLRPTAQDLATLPCRRVKIFLAEKDSLKFGGWNYDEDLKSSGWNGAVETVEHG 298
            + S++        DL+ L C +V + +AEKD+L   GW Y   L+ SGW G VE VE  
Sbjct: 243 VVQSESV-------DLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESE 302

BLAST of Cp4.1LG16g02120 vs. ExPASy Swiss-Prot
Match: Q9LMA7 (Probable carboxylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=CXE1 PE=2 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 2.1e-62
Identity = 123/313 (39.30%), Postives = 182/313 (58.15%), Query Frame = 0

Query: 6   NDVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSALSVRIFIP 65
           +++A ++ P  RI+K+G I+RL   + + P+   +P++   +KD   S +  LS+RI++P
Sbjct: 3   SEIAFDYSPRFRIFKNGGIERLVPETFVPPSL--NPENGVVSKDAVYSPEKNLSLRIYLP 62

Query: 66  --SAVDLNQ-KLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPENP 125
             S  +  + K+PLL+Y HGG F  E+AF+  YH+ + S  +  + +AVSVEYR APE+P
Sbjct: 63  QNSVYETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEHP 122

Query: 126 IPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGSSA 185
           IP  Y+D WDA++WI  H+ R GPE WLN+HAD  ++ LAGDSAGANI HH+A+R     
Sbjct: 123 IPTLYEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEK 182

Query: 186 EQLGRVKVVALALIHPFFGDGA------------GDRLWKYLCSDT------TWLRPTAQ 245
                 K+  + L HP+F   A             +RLW+    D+       W+     
Sbjct: 183 LPPENFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINVVGS 242

Query: 246 DLATLPCRRVKIFLAEKDSLKFGGWNYDEDLKSSGWNGAVETVEHGGEDHVFHLKMPECE 298
           DL  L CRRV + +A  D L  GGW+Y  +L+ SGW G V+ +E   E HVFHL+ P+ E
Sbjct: 243 DLTGLGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRDPDSE 302

BLAST of Cp4.1LG16g02120 vs. ExPASy Swiss-Prot
Match: Q9FX94 (Probable carboxylesterase 5 OS=Arabidopsis thaliana OX=3702 GN=CXE5 PE=2 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 3.5e-62
Identity = 127/319 (39.81%), Postives = 188/319 (58.93%), Query Frame = 0

Query: 6   NDVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSALSVRIFIP 65
           +++A EF PF RIYKDGR++RL + +D +PA   DP     +KDV  S ++ LSVR+F+P
Sbjct: 3   SEIASEFLPFCRIYKDGRVERL-IGTDTIPASL-DPTYDVVSKDVIYSPENNLSVRLFLP 62

Query: 66  ---SAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPENP 125
              + +    KLPLL+Y+HGGA+  ES F+  YH+++  +   AN +AVSV+YR APE+P
Sbjct: 63  HKSTKLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDP 122

Query: 126 IPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGSSA 185
           +PA Y+D W A++WI AH N  GP  W+N+HAD  ++ L GDSAG NI HH+A++AG   
Sbjct: 123 VPAAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGK-- 182

Query: 186 EQLGRVKVVALALIHP-FFGDGAGD--------------RLWKYLCSDTTW--------- 245
           E+   +K+  +A++HP F+G    D               +W+ + S  +          
Sbjct: 183 EKKLDLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFN 242

Query: 246 LRPTAQDLATLPCRRVKIFLAEKDSLKFGGWNYDEDLKSSGWNGAVETVEHGGEDHVFHL 298
           +  +  D + L C +V + +A KD     G  Y   L+   W G VE VE  GEDHVFHL
Sbjct: 243 VNGSGSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHL 302

BLAST of Cp4.1LG16g02120 vs. ExPASy Swiss-Prot
Match: Q5NUF4 (2-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.3e-60
Identity = 122/329 (37.08%), Postives = 191/329 (58.05%), Query Frame = 0

Query: 1   MASSTN-----DVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVD 60
           MASST+     ++  E  P LR+YKDG ++R  + S  VP   +DP++    KD+ IS +
Sbjct: 1   MASSTSTTTSKEIDRELPPLLRVYKDGTVERF-LGSSFVPPSPEDPETGVSTKDIVISEN 60

Query: 61  SALSVRIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEY 120
             +S R+++P   +  +KLP+L+Y HGGAFC ESAF+  +  ++  +A+KAN + VS+EY
Sbjct: 61  PTISARVYLPKLNNTTEKLPILVYYHGGAFCLESAFSFLHQRYLNIVASKANVLVVSIEY 120

Query: 121 RLAPENPIPACYDDCWDALRWIAAH-VNRDGP---EPWLNQHADLDRICLAGDSAGANIC 180
           RLAPE+P+PA Y+D W AL+W+ +H  N + P   +PWL +H D +R  + GD++GANI 
Sbjct: 121 RLAPEHPLPAAYEDGWYALKWVTSHSTNNNKPTNADPWLIKHGDFNRFYIGGDTSGANIA 180

Query: 181 HHLAVRAGSSAEQLGRVKVVALALIHPFF--------------GDGAGDRLWKYLCSDTT 240
           H+ A+R G+ A   G +++  +    P F                 +  ++W ++  D  
Sbjct: 181 HNAALRVGAEALP-GGLRIAGVLSAFPLFWGSKPVLSEPVEGHEKSSPMQVWNFVYPDAP 240

Query: 241 W---------LRPTAQDLATLPCRRVKIFLAEKDSLKFGGWNYDEDLKSSGWNGAVETVE 298
                     L P A +LATL C ++ +F+A KD L+  G  Y E +K SGW G VE  +
Sbjct: 241 GGIDNPLINPLAPGAPNLATLGCPKMLVFVAGKDDLRDRGIWYYEAVKESGWKGDVELAQ 300

BLAST of Cp4.1LG16g02120 vs. ExPASy Swiss-Prot
Match: Q9SMM9 (Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 5.7e-60
Identity = 128/332 (38.55%), Postives = 188/332 (56.63%), Query Frame = 0

Query: 6   NDVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSALSVRIFIP 65
           +++A ++ P L IYK GRI+RL   + + P  + +P++   +KDV  S D+ LS+RI++P
Sbjct: 3   SEIAADYSPMLIIYKSGRIERLVGETTVPP--SSNPQNGVVSKDVVYSPDNNLSLRIYLP 62

Query: 66  -----SAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 125
                +  + + KLPLL+Y HGG F  E+AF+  YH+ + +  + ++ VAVSV+YR APE
Sbjct: 63  EKAATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPE 122

Query: 126 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGS 185
           +PIP  YDD W AL+W+ +H+   G E WLN+HAD  ++ LAGDSAGANI HH+ ++A  
Sbjct: 123 HPIPTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAK 182

Query: 186 ---SAEQLGRVKVVALALIHPFFGD----------GAGDRLWKYLCSDTTW--------- 245
              S E L    +  + L+HP+F                R W     ++ W         
Sbjct: 183 DKLSPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTW----IESVWTLASPNSKD 242

Query: 246 ---------LRPTAQDLATLPCRRVKIFLAEKDSLKFGGWNYDEDLKSSGWNGAV-ETVE 301
                    ++  + DL+ L C +V + +AEKD+L   GW Y E L  S WNG V + VE
Sbjct: 243 GSDDPFINVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVE 302

BLAST of Cp4.1LG16g02120 vs. NCBI nr
Match: XP_023512082.1 (probable carboxylesterase 12 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 627 bits (1617), Expect = 4.60e-227
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0

Query: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSALSV 60
           MASSTNDVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSALSV
Sbjct: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSALSV 60

Query: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120
           RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE
Sbjct: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120

Query: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGS 180
           NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGS
Sbjct: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGS 180

Query: 181 SAEQLGRVKVVALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240
           SAEQLGRVKVVALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE
Sbjct: 181 SAEQLGRVKVVALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240

Query: 241 KDSLKFGGWNYDEDLKSSGWNGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFINLD 300
           KDSLKFGGWNYDEDLKSSGWNGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFINLD
Sbjct: 241 KDSLKFGGWNYDEDLKSSGWNGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFINLD 300

BLAST of Cp4.1LG16g02120 vs. NCBI nr
Match: XP_022986577.1 (probable carboxylesterase 12 [Cucurbita maxima] >XP_022986578.1 probable carboxylesterase 12 [Cucurbita maxima])

HSP 1 Score: 611 bits (1576), Expect = 8.20e-221
Identity = 294/300 (98.00%), Postives = 296/300 (98.67%), Query Frame = 0

Query: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSALSV 60
           MASSTNDVADEFFPFLRIYK+GRIQRLQVTSDIVPACADDP SAFRAKDVTISVDSALSV
Sbjct: 1   MASSTNDVADEFFPFLRIYKNGRIQRLQVTSDIVPACADDPNSAFRAKDVTISVDSALSV 60

Query: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120
           RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE
Sbjct: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120

Query: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGS 180
           NPIPACYDDCWDALRWIAAHVNRDGPEPWLN+HADLDRICLAGDSAGANICHHLAVRAGS
Sbjct: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNEHADLDRICLAGDSAGANICHHLAVRAGS 180

Query: 181 SAEQLGRVKVVALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240
           SAEQLGRVKVVALALIHPFFGDGA DRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE
Sbjct: 181 SAEQLGRVKVVALALIHPFFGDGAEDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240

Query: 241 KDSLKFGGWNYDEDLKSSGWNGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFINLD 300
           KDSLKFGG NYDEDLKSSGW GAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFINLD
Sbjct: 241 KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFINLD 300

BLAST of Cp4.1LG16g02120 vs. NCBI nr
Match: XP_022944136.1 (probable carboxylesterase 12 [Cucurbita moschata])

HSP 1 Score: 610 bits (1573), Expect = 2.35e-220
Identity = 293/300 (97.67%), Postives = 295/300 (98.33%), Query Frame = 0

Query: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSALSV 60
           MASSTNDVADEFFPFLRIYKDGRIQRLQVTSDIV ACADDP SAFRAKDVTISVDSALSV
Sbjct: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVTSDIVSACADDPNSAFRAKDVTISVDSALSV 60

Query: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120
           RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYH HVGSLAAKANAVAVSVEYRLAPE
Sbjct: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHRHVGSLAAKANAVAVSVEYRLAPE 120

Query: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGS 180
           NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAG+
Sbjct: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGT 180

Query: 181 SAEQLGRVKVVALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240
           SAEQLGRVKVVALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE
Sbjct: 181 SAEQLGRVKVVALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240

Query: 241 KDSLKFGGWNYDEDLKSSGWNGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFINLD 300
           KDSLKFGG NYDEDLKSSGW GAVETVEHGGEDHVFHLKMPECEKAVDLMEKL+CFINLD
Sbjct: 241 KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFINLD 300

BLAST of Cp4.1LG16g02120 vs. NCBI nr
Match: KAG6571158.1 (putative carboxylesterase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 609 bits (1570), Expect = 6.74e-220
Identity = 292/300 (97.33%), Postives = 294/300 (98.00%), Query Frame = 0

Query: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSALSV 60
           MASSTNDVADEFFPFLRIYKDGRIQRLQVTSDIV ACADDP SAFRAKDVTISVDSALSV
Sbjct: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVTSDIVSACADDPSSAFRAKDVTISVDSALSV 60

Query: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120
           RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE
Sbjct: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120

Query: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGS 180
           NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAG+
Sbjct: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGT 180

Query: 181 SAEQLGRVKVVALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240
           S EQLGRVKV ALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE
Sbjct: 181 SGEQLGRVKVAALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240

Query: 241 KDSLKFGGWNYDEDLKSSGWNGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFINLD 300
           KDSLKFGG NYDEDLKSSGW GAVETVEHGGEDHVFHLKMPECEKAVDLMEKL+CFINLD
Sbjct: 241 KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFINLD 300

BLAST of Cp4.1LG16g02120 vs. NCBI nr
Match: KAG7010967.1 (putative carboxylesterase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 607 bits (1565), Expect = 3.90e-219
Identity = 291/300 (97.00%), Postives = 293/300 (97.67%), Query Frame = 0

Query: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSALSV 60
           MASSTNDVADEFFPFLRIYKDGRIQRLQV SDIV ACADDP SAFRAKDVTISVDSALSV
Sbjct: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVASDIVSACADDPNSAFRAKDVTISVDSALSV 60

Query: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120
           RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE
Sbjct: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120

Query: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGS 180
           NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAG+
Sbjct: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGT 180

Query: 181 SAEQLGRVKVVALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240
           S EQLGRVKV ALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE
Sbjct: 181 SGEQLGRVKVAALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240

Query: 241 KDSLKFGGWNYDEDLKSSGWNGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFINLD 300
           KDSLKFGG NYDEDLKSSGW GAVETVEHGGEDHVFHLKMPECEKAVDLMEKL+CFINLD
Sbjct: 241 KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFINLD 300

BLAST of Cp4.1LG16g02120 vs. ExPASy TrEMBL
Match: A0A6J1J7Y5 (probable carboxylesterase 12 OS=Cucurbita maxima OX=3661 GN=LOC111484278 PE=4 SV=1)

HSP 1 Score: 611 bits (1576), Expect = 3.97e-221
Identity = 294/300 (98.00%), Postives = 296/300 (98.67%), Query Frame = 0

Query: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSALSV 60
           MASSTNDVADEFFPFLRIYK+GRIQRLQVTSDIVPACADDP SAFRAKDVTISVDSALSV
Sbjct: 1   MASSTNDVADEFFPFLRIYKNGRIQRLQVTSDIVPACADDPNSAFRAKDVTISVDSALSV 60

Query: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120
           RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE
Sbjct: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120

Query: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGS 180
           NPIPACYDDCWDALRWIAAHVNRDGPEPWLN+HADLDRICLAGDSAGANICHHLAVRAGS
Sbjct: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNEHADLDRICLAGDSAGANICHHLAVRAGS 180

Query: 181 SAEQLGRVKVVALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240
           SAEQLGRVKVVALALIHPFFGDGA DRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE
Sbjct: 181 SAEQLGRVKVVALALIHPFFGDGAEDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240

Query: 241 KDSLKFGGWNYDEDLKSSGWNGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFINLD 300
           KDSLKFGG NYDEDLKSSGW GAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFINLD
Sbjct: 241 KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFINLD 300

BLAST of Cp4.1LG16g02120 vs. ExPASy TrEMBL
Match: A0A6J1FTL3 (probable carboxylesterase 12 OS=Cucurbita moschata OX=3662 GN=LOC111448682 PE=4 SV=1)

HSP 1 Score: 610 bits (1573), Expect = 1.14e-220
Identity = 293/300 (97.67%), Postives = 295/300 (98.33%), Query Frame = 0

Query: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSALSV 60
           MASSTNDVADEFFPFLRIYKDGRIQRLQVTSDIV ACADDP SAFRAKDVTISVDSALSV
Sbjct: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVTSDIVSACADDPNSAFRAKDVTISVDSALSV 60

Query: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120
           RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYH HVGSLAAKANAVAVSVEYRLAPE
Sbjct: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHRHVGSLAAKANAVAVSVEYRLAPE 120

Query: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGS 180
           NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAG+
Sbjct: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGT 180

Query: 181 SAEQLGRVKVVALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240
           SAEQLGRVKVVALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE
Sbjct: 181 SAEQLGRVKVVALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIFLAE 240

Query: 241 KDSLKFGGWNYDEDLKSSGWNGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFINLD 300
           KDSLKFGG NYDEDLKSSGW GAVETVEHGGEDHVFHLKMPECEKAVDLMEKL+CFINLD
Sbjct: 241 KDSLKFGGRNYDEDLKSSGWKGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLSCFINLD 300

BLAST of Cp4.1LG16g02120 vs. ExPASy TrEMBL
Match: A0A0A0LNZ1 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G234530 PE=4 SV=1)

HSP 1 Score: 503 bits (1294), Expect = 4.21e-178
Identity = 241/303 (79.54%), Postives = 264/303 (87.13%), Query Frame = 0

Query: 1   MASSTN---DVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSA 60
           MASSTN   DVA +FFPFLR+Y DGR+QRL  TSDIVPA ADDPKS FR+KDVTIS D A
Sbjct: 1   MASSTNKNDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPA 60

Query: 61  LSVRIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRL 120
           +S R+FIPS+ D NQKLPLLLYVHGGAFC ESAF+LQYH HVGSLAAKANAVAVSVEYRL
Sbjct: 61  VSARVFIPSSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRL 120

Query: 121 APENPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVR 180
           APE+PIPACY+DCWDALRW+AAHVNRDG EPWLN + D +RICLAGDSAGANICH+LA R
Sbjct: 121 APEHPIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAAR 180

Query: 181 AGSSAEQLGRVKVVALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIF 240
           A SSAE+LG  KVVA+ALIHPFFGDG  +RLWKYLCS+T  LRPT +DLA L C+RVKIF
Sbjct: 181 ASSSAEELGGAKVVAMALIHPFFGDGGENRLWKYLCSETKLLRPTIEDLAKLGCKRVKIF 240

Query: 241 LAEKDSLKFGGWNYDEDLKSSGWNGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFI 300
           LAE D LK GG NY+EDLKSSGWNG VETVEHG E+HVFHLK PECEKAVDL+EKLA FI
Sbjct: 241 LAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPECEKAVDLLEKLASFI 300

BLAST of Cp4.1LG16g02120 vs. ExPASy TrEMBL
Match: A0A5D3CP06 (2-hydroxyisoflavanone dehydratase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G00380 PE=4 SV=1)

HSP 1 Score: 495 bits (1275), Expect = 3.30e-175
Identity = 235/303 (77.56%), Postives = 262/303 (86.47%), Query Frame = 0

Query: 1   MASSTN---DVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSA 60
           MASSTN   +VA + FPFLR+Y DGR+QRL +TSD VPA ADDPKS FR+KDVTIS D A
Sbjct: 1   MASSTNKNNEVAFDLFPFLRVYSDGRVQRLMITSDFVPADADDPKSPFRSKDVTISTDPA 60

Query: 61  LSVRIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRL 120
           +S R+FIPS+ D NQKLPLLLYVHGGAFC ESAFNLQYH HVG+LAA+ANAVAVSVEYRL
Sbjct: 61  VSARVFIPSSADPNQKLPLLLYVHGGAFCIESAFNLQYHQHVGALAAEANAVAVSVEYRL 120

Query: 121 APENPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVR 180
           APE+PIPACYDDCWDALRW+AAHVNRDG EPWLN + D +RICLAGDSAGANICH+LA R
Sbjct: 121 APEHPIPACYDDCWDALRWVAAHVNRDGLEPWLNTYVDFNRICLAGDSAGANICHYLAAR 180

Query: 181 AGSSAEQLGRVKVVALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIF 240
           A SS+E+LG  KVVA+ALIHPFFGDG  DRLWKYLCS+T  +RPT ++LA + C+RVKIF
Sbjct: 181 ASSSSEELGGAKVVAMALIHPFFGDGREDRLWKYLCSETRLVRPTKEELAKMGCKRVKIF 240

Query: 241 LAEKDSLKFGGWNYDEDLKSSGWNGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFI 300
           LAE D LKFGG NYDEDLK SGWNG VETVEHGGE+HVFHLK PE E AVDL+EKLA FI
Sbjct: 241 LAENDFLKFGGRNYDEDLKRSGWNGVVETVEHGGENHVFHLKNPESENAVDLLEKLASFI 300

BLAST of Cp4.1LG16g02120 vs. ExPASy TrEMBL
Match: A0A1S3C9R7 (2-hydroxyisoflavanone dehydratase-like OS=Cucumis melo OX=3656 GN=LOC103498401 PE=4 SV=1)

HSP 1 Score: 495 bits (1275), Expect = 3.30e-175
Identity = 235/303 (77.56%), Postives = 262/303 (86.47%), Query Frame = 0

Query: 1   MASSTN---DVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSA 60
           MASSTN   +VA + FPFLR+Y DGR+QRL +TSD VPA ADDPKS FR+KDVTIS D A
Sbjct: 1   MASSTNKNNEVAFDLFPFLRVYSDGRVQRLMITSDFVPADADDPKSPFRSKDVTISTDPA 60

Query: 61  LSVRIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRL 120
           +S R+FIPS+ D NQKLPLLLYVHGGAFC ESAFNLQYH HVG+LAA+ANAVAVSVEYRL
Sbjct: 61  VSARVFIPSSADPNQKLPLLLYVHGGAFCIESAFNLQYHQHVGALAAEANAVAVSVEYRL 120

Query: 121 APENPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVR 180
           APE+PIPACYDDCWDALRW+AAHVNRDG EPWLN + D +RICLAGDSAGANICH+LA R
Sbjct: 121 APEHPIPACYDDCWDALRWVAAHVNRDGLEPWLNTYVDFNRICLAGDSAGANICHYLAAR 180

Query: 181 AGSSAEQLGRVKVVALALIHPFFGDGAGDRLWKYLCSDTTWLRPTAQDLATLPCRRVKIF 240
           A SS+E+LG  KVVA+ALIHPFFGDG  DRLWKYLCS+T  +RPT ++LA + C+RVKIF
Sbjct: 181 ASSSSEELGGAKVVAMALIHPFFGDGREDRLWKYLCSETRLVRPTKEELAKMGCKRVKIF 240

Query: 241 LAEKDSLKFGGWNYDEDLKSSGWNGAVETVEHGGEDHVFHLKMPECEKAVDLMEKLACFI 300
           LAE D LKFGG NYDEDLK SGWNG VETVEHGGE+HVFHLK PE E AVDL+EKLA FI
Sbjct: 241 LAENDFLKFGGRNYDEDLKRSGWNGVVETVEHGGENHVFHLKNPESENAVDLLEKLASFI 300

BLAST of Cp4.1LG16g02120 vs. TAIR 10
Match: AT3G48690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 249.2 bits (635), Expect = 4.2e-66
Identity = 131/327 (40.06%), Postives = 188/327 (57.49%), Query Frame = 0

Query: 6   NDVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSALSVRIFIP 65
           +++A +  P L+IYK GRI+RL   + + P  + +P++   +KDV  S D+ LSVRI++P
Sbjct: 3   SEIAVDCSPLLKIYKSGRIERLMGEATVPP--SSEPQNGVVSKDVVYSADNNLSVRIYLP 62

Query: 66  --SAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPENPI 125
             +A + + KLPLL+Y HGG F  E+AF+  YH+ + +  + +N VAVSV+YR APE+PI
Sbjct: 63  EKAAAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPI 122

Query: 126 PACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGSS-- 185
              +DD W AL+W+  H+   G E WLN+HAD  R+ L+GDSAGANI HH+A+RA     
Sbjct: 123 SVPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKL 182

Query: 186 AEQLGRVKVVALALIHPFF-------------------------------GDGAGDRLWK 245
           +  L    +  + L+HP+F                                DG  D L  
Sbjct: 183 SPGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLN 242

Query: 246 YLCSDTTWLRPTAQDLATLPCRRVKIFLAEKDSLKFGGWNYDEDLKSSGWNGAVETVEHG 298
            + S++        DL+ L C +V + +AEKD+L   GW Y   L+ SGW G VE VE  
Sbjct: 243 VVQSESV-------DLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESE 302

BLAST of Cp4.1LG16g02120 vs. TAIR 10
Match: AT1G19190.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 240.7 bits (613), Expect = 1.5e-63
Identity = 123/313 (39.30%), Postives = 182/313 (58.15%), Query Frame = 0

Query: 6   NDVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSALSVRIFIP 65
           +++A ++ P  RI+K+G I+RL   + + P+   +P++   +KD   S +  LS+RI++P
Sbjct: 3   SEIAFDYSPRFRIFKNGGIERLVPETFVPPSL--NPENGVVSKDAVYSPEKNLSLRIYLP 62

Query: 66  --SAVDLNQ-KLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPENP 125
             S  +  + K+PLL+Y HGG F  E+AF+  YH+ + S  +  + +AVSVEYR APE+P
Sbjct: 63  QNSVYETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEHP 122

Query: 126 IPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGSSA 185
           IP  Y+D WDA++WI  H+ R GPE WLN+HAD  ++ LAGDSAGANI HH+A+R     
Sbjct: 123 IPTLYEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEK 182

Query: 186 EQLGRVKVVALALIHPFFGDGA------------GDRLWKYLCSDT------TWLRPTAQ 245
                 K+  + L HP+F   A             +RLW+    D+       W+     
Sbjct: 183 LPPENFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINVVGS 242

Query: 246 DLATLPCRRVKIFLAEKDSLKFGGWNYDEDLKSSGWNGAVETVEHGGEDHVFHLKMPECE 298
           DL  L CRRV + +A  D L  GGW+Y  +L+ SGW G V+ +E   E HVFHL+ P+ E
Sbjct: 243 DLTGLGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRDPDSE 302

BLAST of Cp4.1LG16g02120 vs. TAIR 10
Match: AT1G49660.1 (carboxyesterase 5 )

HSP 1 Score: 240.0 bits (611), Expect = 2.5e-63
Identity = 127/319 (39.81%), Postives = 188/319 (58.93%), Query Frame = 0

Query: 6   NDVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSALSVRIFIP 65
           +++A EF PF RIYKDGR++RL + +D +PA   DP     +KDV  S ++ LSVR+F+P
Sbjct: 3   SEIASEFLPFCRIYKDGRVERL-IGTDTIPASL-DPTYDVVSKDVIYSPENNLSVRLFLP 62

Query: 66  ---SAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPENP 125
              + +    KLPLL+Y+HGGA+  ES F+  YH+++  +   AN +AVSV+YR APE+P
Sbjct: 63  HKSTKLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDP 122

Query: 126 IPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGSSA 185
           +PA Y+D W A++WI AH N  GP  W+N+HAD  ++ L GDSAG NI HH+A++AG   
Sbjct: 123 VPAAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGK-- 182

Query: 186 EQLGRVKVVALALIHP-FFGDGAGD--------------RLWKYLCSDTTW--------- 245
           E+   +K+  +A++HP F+G    D               +W+ + S  +          
Sbjct: 183 EKKLDLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFN 242

Query: 246 LRPTAQDLATLPCRRVKIFLAEKDSLKFGGWNYDEDLKSSGWNGAVETVEHGGEDHVFHL 298
           +  +  D + L C +V + +A KD     G  Y   L+   W G VE VE  GEDHVFHL
Sbjct: 243 VNGSGSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHL 302

BLAST of Cp4.1LG16g02120 vs. TAIR 10
Match: AT1G47480.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 232.6 bits (592), Expect = 4.0e-61
Identity = 133/320 (41.56%), Postives = 182/320 (56.88%), Query Frame = 0

Query: 1   MASSTNDVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSALSV 60
           M S+   V+ E  P+L ++ DG ++RL  T    P    DP +   +KD+ I   + LS 
Sbjct: 1   MESTKKQVSLELLPWLVVHTDGTVERLAGTEVCPPGL--DPITGVFSKDIIIEPKTGLSA 60

Query: 61  RIFIPSAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 120
           RI+ P ++   QK+PL+LY HGGAF   S     YH+ +  +  +AN +AVSV YRLAPE
Sbjct: 61  RIYRPFSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPE 120

Query: 121 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGS 180
           +P+P  Y+D W AL+ I A +N    EPW+N +ADLD + L GDSAGANI HHLA RA  
Sbjct: 121 HPLPTAYEDSWTALKNIQA-IN----EPWINDYADLDSLFLVGDSAGANISHHLAFRAKQ 180

Query: 181 SAEQLGRVKVVALALIHPFFGD----GAG----------DRLWKYLC-----SDTTWLRP 240
           S + L   K+  + +IHP+F      GA           D  W+++C     SD  W+ P
Sbjct: 181 SDQTL---KIKGIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINP 240

Query: 241 TAQ---DLATLPCRRVKIFLAEKDSLKFGGWNYDEDLKSSGWNGAVETVEHGGEDHVFHL 299
            A    DL  L C RV I +AEKD L   G  Y E L  S W G VE +E   +DHVFH+
Sbjct: 241 FADGSPDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHI 300

BLAST of Cp4.1LG16g02120 vs. TAIR 10
Match: AT3G48700.1 (carboxyesterase 13 )

HSP 1 Score: 232.6 bits (592), Expect = 4.0e-61
Identity = 128/332 (38.55%), Postives = 188/332 (56.63%), Query Frame = 0

Query: 6   NDVADEFFPFLRIYKDGRIQRLQVTSDIVPACADDPKSAFRAKDVTISVDSALSVRIFIP 65
           +++A ++ P L IYK GRI+RL   + + P  + +P++   +KDV  S D+ LS+RI++P
Sbjct: 3   SEIAADYSPMLIIYKSGRIERLVGETTVPP--SSNPQNGVVSKDVVYSPDNNLSLRIYLP 62

Query: 66  -----SAVDLNQKLPLLLYVHGGAFCFESAFNLQYHSHVGSLAAKANAVAVSVEYRLAPE 125
                +  + + KLPLL+Y HGG F  E+AF+  YH+ + +  + ++ VAVSV+YR APE
Sbjct: 63  EKAATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPE 122

Query: 126 NPIPACYDDCWDALRWIAAHVNRDGPEPWLNQHADLDRICLAGDSAGANICHHLAVRAGS 185
           +PIP  YDD W AL+W+ +H+   G E WLN+HAD  ++ LAGDSAGANI HH+ ++A  
Sbjct: 123 HPIPTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAK 182

Query: 186 ---SAEQLGRVKVVALALIHPFFGD----------GAGDRLWKYLCSDTTW--------- 245
              S E L    +  + L+HP+F                R W     ++ W         
Sbjct: 183 DKLSPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTW----IESVWTLASPNSKD 242

Query: 246 ---------LRPTAQDLATLPCRRVKIFLAEKDSLKFGGWNYDEDLKSSGWNGAV-ETVE 301
                    ++  + DL+ L C +V + +AEKD+L   GW Y E L  S WNG V + VE
Sbjct: 243 GSDDPFINVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVE 302

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SMN05.8e-6540.06Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1[more]
Q9LMA72.1e-6239.30Probable carboxylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=CXE1 PE=2 SV=1[more]
Q9FX943.5e-6239.81Probable carboxylesterase 5 OS=Arabidopsis thaliana OX=3702 GN=CXE5 PE=2 SV=1[more]
Q5NUF43.3e-6037.082-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 ... [more]
Q9SMM95.7e-6038.55Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_023512082.14.60e-227100.00probable carboxylesterase 12 [Cucurbita pepo subsp. pepo][more]
XP_022986577.18.20e-22198.00probable carboxylesterase 12 [Cucurbita maxima] >XP_022986578.1 probable carboxy... [more]
XP_022944136.12.35e-22097.67probable carboxylesterase 12 [Cucurbita moschata][more]
KAG6571158.16.74e-22097.33putative carboxylesterase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7010967.13.90e-21997.00putative carboxylesterase 2, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
Match NameE-valueIdentityDescription
A0A6J1J7Y53.97e-22198.00probable carboxylesterase 12 OS=Cucurbita maxima OX=3661 GN=LOC111484278 PE=4 SV... [more]
A0A6J1FTL31.14e-22097.67probable carboxylesterase 12 OS=Cucurbita moschata OX=3662 GN=LOC111448682 PE=4 ... [more]
A0A0A0LNZ14.21e-17879.54Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G2345... [more]
A0A5D3CP063.30e-17577.562-hydroxyisoflavanone dehydratase-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3C9R73.30e-17577.562-hydroxyisoflavanone dehydratase-like OS=Cucumis melo OX=3656 GN=LOC103498401 P... [more]
Match NameE-valueIdentityDescription
AT3G48690.14.2e-6640.06alpha/beta-Hydrolases superfamily protein [more]
AT1G19190.11.5e-6339.30alpha/beta-Hydrolases superfamily protein [more]
AT1G49660.12.5e-6339.81carboxyesterase 5 [more]
AT1G47480.14.0e-6141.56alpha/beta-Hydrolases superfamily protein [more]
AT3G48700.14.0e-6138.55carboxyesterase 13 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 77..199
e-value: 4.8E-35
score: 121.3
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 15..299
e-value: 4.9E-62
score: 212.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 15..298
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 5..298
NoneNo IPR availablePANTHERPTHR23024:SF514CARBOXYLESTERASE 12-RELATEDcoord: 5..298
IPR033140Lipase, GDXG, putative serine active sitePROSITEPS01174LIPASE_GDXG_SERcoord: 159..171

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG16g02120.1Cp4.1LG16g02120.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity