Cp4.1LG16g01080 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG16g01080
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptiondefensin-like protein 1
LocationCp4.1LG16: 2109294 .. 2109600 (-)
RNA-Seq ExpressionCp4.1LG16g01080
SyntenyCp4.1LG16g01080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGAACAAATCCTTCCTCTCCATGCTCCTATTGGTCCTCCTCATCTTCTCTTCTTCAGGTAATCAAAGTCTAACCCTACCATCTTTGCCATTTCTTCTTTCTTCCTTTCTTCCTAATATGTGTGTTATTTGCAGAAGAGATGATGGGTCGTGGTGCTGAGGCTCGAATATGCAAGTCCAGGAACCACCACTACCATGGCCTCTGCTTTAGCCACAAGTGCGCTACTCGTTGCCGAAAGGAAGGCTACCTTGGCGGTCATTGTCACCACTTCCCCCGCCATTGCTATTGCACTCACAGTTGTTAA

mRNA sequence

ATGATGAACAAATCCTTCCTCTCCATGCTCCTATTGGTCCTCCTCATCTTCTCTTCTTCAGAAGAGATGATGGGTCGTGGTGCTGAGGCTCGAATATGCAAGTCCAGGAACCACCACTACCATGGCCTCTGCTTTAGCCACAAGTGCGCTACTCGTTGCCGAAAGGAAGGCTACCTTGGCGGTCATTGTCACCACTTCCCCCGCCATTGCTATTGCACTCACAGTTGTTAA

Coding sequence (CDS)

ATGATGAACAAATCCTTCCTCTCCATGCTCCTATTGGTCCTCCTCATCTTCTCTTCTTCAGAAGAGATGATGGGTCGTGGTGCTGAGGCTCGAATATGCAAGTCCAGGAACCACCACTACCATGGCCTCTGCTTTAGCCACAAGTGCGCTACTCGTTGCCGAAAGGAAGGCTACCTTGGCGGTCATTGTCACCACTTCCCCCGCCATTGCTATTGCACTCACAGTTGTTAA

Protein sequence

MMNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFSHKCATRCRKEGYLGGHCHHFPRHCYCTHSC
Homology
BLAST of Cp4.1LG16g01080 vs. ExPASy Swiss-Prot
Match: Q9FFP8 (Defensin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=PDF2.5 PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.2e-11
Identity = 35/76 (46.05%), Postives = 46/76 (60.53%), Query Frame = 0

Query: 2  MNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFS-HKCATRCRKEGYLG 61
          M   F +   L+L++FSS E +   G E R C+S++HH+  +C S H CA  CR EG+ G
Sbjct: 1  MENKFFAAFFLLLVLFSSQEII---GGEGRTCQSKSHHFKYMCTSNHNCAIVCRNEGFSG 60

Query: 62 GHCHHFPRHCYCTHSC 77
          G CH F R CYCT  C
Sbjct: 61 GRCHGFHRRCYCTRLC 73

BLAST of Cp4.1LG16g01080 vs. ExPASy Swiss-Prot
Match: Q40901 (Defensin-like protein OS=Petunia integrifolia OX=4103 PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 2.0e-09
Identity = 28/66 (42.42%), Postives = 43/66 (65.15%), Query Frame = 0

Query: 12 LVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCF-SHKCATRCRKEGYLGGHCHHFPRHC 71
          L+ ++F S+E      AEAR C+S++H +HG C     CA+ C+ EG++GG+C  F R C
Sbjct: 13 LIAMLFLSTEMGPMTSAEARTCESQSHRFHGTCVRESNCASVCQTEGFIGGNCRAFRRRC 72

Query: 72 YCTHSC 77
          +CT +C
Sbjct: 73 FCTRNC 78

BLAST of Cp4.1LG16g01080 vs. ExPASy Swiss-Prot
Match: Q39182 (Defensin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PDF2.2 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 9.1e-07
Identity = 29/79 (36.71%), Postives = 46/79 (58.23%), Query Frame = 0

Query: 2  MNKSFLSMLLLVLLIFSSSEEMMGRG---AEARICKSRNHHYHGLCFS-HKCATRCRKEG 61
          ++   +S +L++ +IF ++    G G    EAR C+S++H + G C S   CA  C  EG
Sbjct: 3  LSMRLISAVLIMFMIFVAT----GMGPVTVEARTCESQSHRFKGTCVSASNCANVCHNEG 62

Query: 62 YLGGHCHHFPRHCYCTHSC 77
          ++GG+C  F R C+CT  C
Sbjct: 63 FVGGNCRGFRRRCFCTRHC 77

BLAST of Cp4.1LG16g01080 vs. ExPASy Swiss-Prot
Match: P83399 (Defensin-like protein 1 OS=Vigna unguiculata OX=3917 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 3.5e-06
Identity = 22/47 (46.81%), Postives = 30/47 (63.83%), Query Frame = 0

Query: 31 RICKSRNHHYHGLCF-SHKCATRCRKEGYLGGHCHHFPRHCYCTHSC 77
          R+C+S++H + G C   H CA  CR EG+ GG+C  F R C+CT  C
Sbjct: 1  RVCESQSHGFKGACTGDHNCALVCRNEGFSGGNCRGFRRRCFCTLKC 47

BLAST of Cp4.1LG16g01080 vs. ExPASy Swiss-Prot
Match: P20346 (Defensin-like protein P322 OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 7.7e-06
Identity = 29/72 (40.28%), Postives = 41/72 (56.94%), Query Frame = 0

Query: 6  FLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCF-SHKCATRCRKEGYLGGHCH 65
          F +  LL +L+ ++    M R AEAR C+S +H + G C     CA+ C  E + GG+CH
Sbjct: 4  FATFFLLAMLVVATKMGPM-RIAEARHCESLSHRFKGPCTRDSNCASVCETERFSGGNCH 63

Query: 66 HFPRHCYCTHSC 77
           F R C+CT  C
Sbjct: 64 GFRRRCFCTKPC 74

BLAST of Cp4.1LG16g01080 vs. NCBI nr
Match: XP_023513006.1 (defensin-like protein 1 [Cucurbita pepo subsp. pepo] >XP_023513007.1 defensin-like protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 164 bits (415), Expect = 3.95e-51
Identity = 76/76 (100.00%), Postives = 76/76 (100.00%), Query Frame = 0

Query: 1  MMNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFSHKCATRCRKEGYLG 60
          MMNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFSHKCATRCRKEGYLG
Sbjct: 1  MMNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFSHKCATRCRKEGYLG 60

Query: 61 GHCHHFPRHCYCTHSC 76
          GHCHHFPRHCYCTHSC
Sbjct: 61 GHCHHFPRHCYCTHSC 76

BLAST of Cp4.1LG16g01080 vs. NCBI nr
Match: KAG6571315.1 (Defensin-like protein 6, partial [Cucurbita argyrosperma subsp. sororia] >KAG6571316.1 Defensin-like protein 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 162 bits (410), Expect = 2.29e-50
Identity = 75/76 (98.68%), Postives = 75/76 (98.68%), Query Frame = 0

Query: 1  MMNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFSHKCATRCRKEGYLG 60
          MMNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFSHKCATRCRKEGY G
Sbjct: 1  MMNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFSHKCATRCRKEGYTG 60

Query: 61 GHCHHFPRHCYCTHSC 76
          GHCHHFPRHCYCTHSC
Sbjct: 61 GHCHHFPRHCYCTHSC 76

BLAST of Cp4.1LG16g01080 vs. NCBI nr
Match: XP_022932007.1 (defensin-like protein 1 [Cucurbita moschata])

HSP 1 Score: 161 bits (407), Expect = 6.58e-50
Identity = 74/76 (97.37%), Postives = 75/76 (98.68%), Query Frame = 0

Query: 1  MMNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFSHKCATRCRKEGYLG 60
          MMNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFSHKCATRCR+EGY G
Sbjct: 1  MMNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFSHKCATRCRREGYTG 60

Query: 61 GHCHHFPRHCYCTHSC 76
          GHCHHFPRHCYCTHSC
Sbjct: 61 GHCHHFPRHCYCTHSC 76

BLAST of Cp4.1LG16g01080 vs. NCBI nr
Match: XP_022973849.1 (defensin-like protein 1 [Cucurbita maxima] >XP_022973850.1 defensin-like protein 1 [Cucurbita maxima] >XP_022973851.1 defensin-like protein 1 [Cucurbita maxima])

HSP 1 Score: 157 bits (396), Expect = 3.14e-48
Identity = 72/76 (94.74%), Postives = 74/76 (97.37%), Query Frame = 0

Query: 1  MMNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFSHKCATRCRKEGYLG 60
          MMNKSFLS+LLLVLLIFSSSEEMMGRGAEAR+CKSRNHHYHGLCFSHKCATRCRKEGY G
Sbjct: 1  MMNKSFLSVLLLVLLIFSSSEEMMGRGAEARMCKSRNHHYHGLCFSHKCATRCRKEGYPG 60

Query: 61 GHCHHFPRHCYCTHSC 76
          GHCHHFPRHCYCTH C
Sbjct: 61 GHCHHFPRHCYCTHRC 76

BLAST of Cp4.1LG16g01080 vs. NCBI nr
Match: XP_022931975.1 (defensin-like protein 1 [Cucurbita moschata] >XP_022933317.1 defensin-like protein 1 [Cucurbita moschata])

HSP 1 Score: 156 bits (395), Expect = 4.60e-48
Identity = 74/77 (96.10%), Postives = 75/77 (97.40%), Query Frame = 0

Query: 1  MMNKSFLSMLLLVLLIF-SSSEEMMGRGAEARICKSRNHHYHGLCFSHKCATRCRKEGYL 60
          MMNKSFLSMLLLVLLIF SSSEEMMGRGAEARICKSRNHHYHGLCFSHKCATRCR+EGY 
Sbjct: 1  MMNKSFLSMLLLVLLIFFSSSEEMMGRGAEARICKSRNHHYHGLCFSHKCATRCRREGYT 60

Query: 61 GGHCHHFPRHCYCTHSC 76
          GGHCHHFPRHCYCTHSC
Sbjct: 61 GGHCHHFPRHCYCTHSC 77

BLAST of Cp4.1LG16g01080 vs. ExPASy TrEMBL
Match: A0A6J1EVF5 (defensin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111438337 PE=4 SV=1)

HSP 1 Score: 161 bits (407), Expect = 3.18e-50
Identity = 74/76 (97.37%), Postives = 75/76 (98.68%), Query Frame = 0

Query: 1  MMNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFSHKCATRCRKEGYLG 60
          MMNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFSHKCATRCR+EGY G
Sbjct: 1  MMNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFSHKCATRCRREGYTG 60

Query: 61 GHCHHFPRHCYCTHSC 76
          GHCHHFPRHCYCTHSC
Sbjct: 61 GHCHHFPRHCYCTHSC 76

BLAST of Cp4.1LG16g01080 vs. ExPASy TrEMBL
Match: A0A6J1I9S0 (defensin-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111472392 PE=4 SV=1)

HSP 1 Score: 157 bits (396), Expect = 1.52e-48
Identity = 72/76 (94.74%), Postives = 74/76 (97.37%), Query Frame = 0

Query: 1  MMNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFSHKCATRCRKEGYLG 60
          MMNKSFLS+LLLVLLIFSSSEEMMGRGAEAR+CKSRNHHYHGLCFSHKCATRCRKEGY G
Sbjct: 1  MMNKSFLSVLLLVLLIFSSSEEMMGRGAEARMCKSRNHHYHGLCFSHKCATRCRKEGYPG 60

Query: 61 GHCHHFPRHCYCTHSC 76
          GHCHHFPRHCYCTH C
Sbjct: 61 GHCHHFPRHCYCTHRC 76

BLAST of Cp4.1LG16g01080 vs. ExPASy TrEMBL
Match: A0A6J1F0X5 (defensin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111438310 PE=4 SV=1)

HSP 1 Score: 156 bits (395), Expect = 2.23e-48
Identity = 74/77 (96.10%), Postives = 75/77 (97.40%), Query Frame = 0

Query: 1  MMNKSFLSMLLLVLLIF-SSSEEMMGRGAEARICKSRNHHYHGLCFSHKCATRCRKEGYL 60
          MMNKSFLSMLLLVLLIF SSSEEMMGRGAEARICKSRNHHYHGLCFSHKCATRCR+EGY 
Sbjct: 1  MMNKSFLSMLLLVLLIFFSSSEEMMGRGAEARICKSRNHHYHGLCFSHKCATRCRREGYT 60

Query: 61 GGHCHHFPRHCYCTHSC 76
          GGHCHHFPRHCYCTHSC
Sbjct: 61 GGHCHHFPRHCYCTHSC 77

BLAST of Cp4.1LG16g01080 vs. ExPASy TrEMBL
Match: A0A6J1DWX4 (defensin-like protein 1 OS=Momordica charantia OX=3673 GN=LOC111025288 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.71e-25
Identity = 50/77 (64.94%), Postives = 60/77 (77.92%), Query Frame = 0

Query: 2  MNKSFLSMLLLVLLIFSSSEEMMGRG-AEARICKSRNHHYHGLCFS-HKCATRCRKEGYL 61
          MNKSFL  LLL+LLIFS+S+EMMG G AEAR+CKS++HH+HG CFS H CA  CR EG+ 
Sbjct: 1  MNKSFLPTLLLLLLIFSASQEMMGGGVAEARVCKSQSHHFHGACFSDHNCAQVCRTEGFS 60

Query: 62 GGHCHHFPRHCYCTHSC 76
          GG C+ F R C+CT  C
Sbjct: 61 GGDCNGFRRRCFCTRLC 77

BLAST of Cp4.1LG16g01080 vs. ExPASy TrEMBL
Match: A0A6J1EVR5 (defensin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111438308 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.71e-25
Identity = 48/77 (62.34%), Postives = 59/77 (76.62%), Query Frame = 0

Query: 1  MMNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFS-HKCATRCRKEGYL 60
          MMN+S L  LLL+LLIFS+S+E+MGR AEAR+CKS++HH+HG CFS H CA  CR EG+ 
Sbjct: 1  MMNRSLLPTLLLLLLIFSASDELMGRTAEARVCKSQSHHFHGSCFSNHNCAVVCRTEGFS 60

Query: 61 GGHCHHFPRHCYCTHSC 76
          GG C  F R C+CT  C
Sbjct: 61 GGDCVGFRRRCFCTRHC 77

BLAST of Cp4.1LG16g01080 vs. TAIR 10
Match: AT5G63660.1 (Scorpion toxin-like knottin superfamily protein )

HSP 1 Score: 70.1 bits (170), Expect = 8.7e-13
Identity = 35/76 (46.05%), Postives = 46/76 (60.53%), Query Frame = 0

Query: 2  MNKSFLSMLLLVLLIFSSSEEMMGRGAEARICKSRNHHYHGLCFS-HKCATRCRKEGYLG 61
          M   F +   L+L++FSS E +   G E R C+S++HH+  +C S H CA  CR EG+ G
Sbjct: 1  MENKFFAAFFLLLVLFSSQEII---GGEGRTCQSKSHHFKYMCTSNHNCAIVCRNEGFSG 60

Query: 62 GHCHHFPRHCYCTHSC 77
          G CH F R CYCT  C
Sbjct: 61 GRCHGFHRRCYCTRLC 73

BLAST of Cp4.1LG16g01080 vs. TAIR 10
Match: AT2G02100.1 (low-molecular-weight cysteine-rich 69 )

HSP 1 Score: 53.9 bits (128), Expect = 6.5e-08
Identity = 29/79 (36.71%), Postives = 46/79 (58.23%), Query Frame = 0

Query: 2  MNKSFLSMLLLVLLIFSSSEEMMGRG---AEARICKSRNHHYHGLCFS-HKCATRCRKEG 61
          ++   +S +L++ +IF ++    G G    EAR C+S++H + G C S   CA  C  EG
Sbjct: 3  LSMRLISAVLIMFMIFVAT----GMGPVTVEARTCESQSHRFKGTCVSASNCANVCHNEG 62

Query: 62 YLGGHCHHFPRHCYCTHSC 77
          ++GG+C  F R C+CT  C
Sbjct: 63 FVGGNCRGFRRRCFCTRHC 77

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FFP81.2e-1146.05Defensin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=PDF2.5 PE=3 SV=1[more]
Q409012.0e-0942.42Defensin-like protein OS=Petunia integrifolia OX=4103 PE=2 SV=1[more]
Q391829.1e-0736.71Defensin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PDF2.2 PE=2 SV=1[more]
P833993.5e-0646.81Defensin-like protein 1 OS=Vigna unguiculata OX=3917 PE=1 SV=1[more]
P203467.7e-0640.28Defensin-like protein P322 OS=Solanum tuberosum OX=4113 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023513006.13.95e-51100.00defensin-like protein 1 [Cucurbita pepo subsp. pepo] >XP_023513007.1 defensin-li... [more]
KAG6571315.12.29e-5098.68Defensin-like protein 6, partial [Cucurbita argyrosperma subsp. sororia] >KAG657... [more]
XP_022932007.16.58e-5097.37defensin-like protein 1 [Cucurbita moschata][more]
XP_022973849.13.14e-4894.74defensin-like protein 1 [Cucurbita maxima] >XP_022973850.1 defensin-like protein... [more]
XP_022931975.14.60e-4896.10defensin-like protein 1 [Cucurbita moschata] >XP_022933317.1 defensin-like prote... [more]
Match NameE-valueIdentityDescription
A0A6J1EVF53.18e-5097.37defensin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111438337 PE=4 SV=1[more]
A0A6J1I9S01.52e-4894.74defensin-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111472392 PE=4 SV=1[more]
A0A6J1F0X52.23e-4896.10defensin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111438310 PE=4 SV=1[more]
A0A6J1DWX41.71e-2564.94defensin-like protein 1 OS=Momordica charantia OX=3673 GN=LOC111025288 PE=4 SV=1[more]
A0A6J1EVR51.71e-2562.34defensin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111438308 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G63660.18.7e-1346.05Scorpion toxin-like knottin superfamily protein [more]
AT2G02100.16.5e-0836.71low-molecular-weight cysteine-rich 69 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008176Defensin, plantPRINTSPR00288PUROTHIONINcoord: 32..47
score: 35.6
coord: 61..75
score: 41.45
IPR003614Knottin, scorpion toxin-likeSMARTSM00505gth_4coord: 32..76
e-value: 4.7E-5
score: 32.8
IPR036574Knottin, scorpion toxin-like superfamilyGENE3D3.30.30.10coord: 31..76
e-value: 1.3E-12
score: 49.7
IPR036574Knottin, scorpion toxin-like superfamilySUPERFAMILY57095Scorpion toxin-likecoord: 31..76
NoneNo IPR availablePFAMPF00304Gamma-thionincoord: 31..76
e-value: 7.1E-10
score: 39.0
NoneNo IPR availablePANTHERPTHR33147:SF108DEFENSIN-LIKE PROTEIN 6coord: 2..76
NoneNo IPR availablePANTHERPTHR33147DEFENSIN-LIKE PROTEIN 1coord: 2..76

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG16g01080.1Cp4.1LG16g01080.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050832 defense response to fungus
biological_process GO:0031640 killing of cells of other organism
biological_process GO:0006952 defense response