Cp4.1LG15g06250 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG15g06250 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 4.8e-76
Identity = 138/312 (44.23%), Postives = 199/312 (63.78%), Query Frame = 0

Query: 25  RFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFIALIGDN 84
           R N +VA DGSG+ K I +A+AAA     +R+ I++  G Y E+I++    K I  +GD 
Sbjct: 200 RANIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEV--KLKNIMFVGDG 259

Query: 85  AFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQADYTA 144
              TII  ++S G G +T  SAT+ V G+NF+A+ ++  N+AG  NH+AVA+   +D + 
Sbjct: 260 IGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSV 319

Query: 145 YYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYARFSKNEI-VVT 204
           +Y+C F G+QDTLY+     F +ECDIYGTVDFIFG+  V+ Q+CNI+AR   N    +T
Sbjct: 320 FYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLT 379

Query: 205 AQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESFLDDNVQP 264
           AQ +  P + +G    NC VT +S++   ++ +K +LGRPW+QYS  V++++FLD  + P
Sbjct: 380 AQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINP 439

Query: 265 KGWMFWQG-VPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRK-KLAKKFTVEKFINGTD 324
            GWM W G   +N L+YAE+ N GPG++T +RV W G+HV+       +FTV  FI G  
Sbjct: 440 AGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNS 499

Query: 325 WLPETGVPFKPG 334
           WLP T VPF  G
Sbjct: 500 WLPATNVPFTSG 509

BLAST of Cp4.1LG15g06250 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 2.4e-75
Identity = 135/310 (43.55%), Postives = 196/310 (63.23%), Query Frame = 0

Query: 30  VALDGSGHYKRISDAIAAAPDN---SKTRFYIHVTPGIYREHIQIPSTKKFIALIGDNAF 89
           V+ DG+G++  I+ A+AAAP+N   S   F I+VT GIY E+I I   K+++ +IGD   
Sbjct: 267 VSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGIN 326

Query: 90  TTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQADYTAYY 149
            T++  NRS   G++T +SAT  V   NFVA  ++F N+AG + H+AVA+   AD++ +Y
Sbjct: 327 QTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSIFY 386

Query: 150 KCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYAR--FSKNEIVVTA 209
            C F  +QDTLY      F +ECD+YGTVDFIFG+  V+FQ+CN+Y R         +TA
Sbjct: 387 SCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAITA 446

Query: 210 QSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESFLDDNVQPK 269
           Q +  P + +G S QNC +  + ++  +   +K YLGRPW++YS  V+M+S++D  V+P 
Sbjct: 447 QGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPV 506

Query: 270 GWMFWQG-VPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRKKLAKKFTVEKFINGTDWL 329
           GW  W G   ++ L+YAE+NN GPG+NT +RV W G+HVI    A  FTV       DW+
Sbjct: 507 GWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANFTVTGLFIEADWI 566

Query: 330 PETGVPFKPG 334
            +TGVP+  G
Sbjct: 567 WKTGVPYTSG 576

BLAST of Cp4.1LG15g06250 vs. ExPASy Swiss-Prot
Match: O81301 (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3702 GN=PME40 PE=2 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.2e-74
Identity = 138/314 (43.95%), Postives = 199/314 (63.38%), Query Frame = 0

Query: 21  VDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFIAL 80
           VD  + N +VA +G+G+Y  I +AI+AAP++S+TRF I++  G Y E+I+IP  K  I  
Sbjct: 201 VDETKVNLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMF 260

Query: 81  IGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQA 140
           IGD    T+I  NRS   G++   SAT+ V+G+ F+AK LSF N AG + H+AVA+   +
Sbjct: 261 IGDGIGRTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVALRSSS 320

Query: 141 DYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYAR--FSKN 200
           D +AYY+C F  +QDT+Y+  H  F +ECDIYGTVDFIFG   V+FQ+C++YAR      
Sbjct: 321 DLSAYYRCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQ 380

Query: 201 EIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESFLD 260
           +I+ TAQ ++     +G S  +  +  + ++   +A  K YLGRPW+ YS  V M+SF+D
Sbjct: 381 KIIYTAQGRENSREPTGISIISSRILAAPDLIPVQANFKAYLGRPWQLYSRTVIMKSFID 440

Query: 261 DNVQPKGWMFWQ-GVPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRK-KLAKKFTVEKF 320
           D V P GW+ W+    +  L+Y E+ N GPG+N  +RV W GF  I   + A +F+V  F
Sbjct: 441 DLVDPAGWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPF 500

Query: 321 INGTDWLPETGVPF 331
           I+G  WL  T +PF
Sbjct: 501 IDGNKWLNSTRIPF 514

BLAST of Cp4.1LG15g06250 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 4.5e-74
Identity = 139/320 (43.44%), Postives = 202/320 (63.12%), Query Frame = 0

Query: 19  ACVDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFI 78
           A V    +N  VA+DG+G++  I+DA+ AAP+ S+TRF I++  G Y E++++P  K  I
Sbjct: 213 APVQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMI 272

Query: 79  ALIGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLD 138
             IGD    T+I  NRS   G+ST  + T+ VKG  ++AK +SF NSAG    +AVA   
Sbjct: 273 MFIGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRS 332

Query: 139 QADYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYARFSK- 198
            +D++A+Y+C F G+QDTLY+     F +ECDIYGT+DFIFG+  V+FQ+ ++YAR    
Sbjct: 333 GSDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNP 392

Query: 199 -NEIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESF 258
            ++I  TAQS++Q  + +G S  NC +  + ++   +   K YLGRPWR+YS  V ++SF
Sbjct: 393 GHKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSF 452

Query: 259 LDDNVQPKGWMFW-QGVPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRKKL-AKKFTVE 318
           +DD + P GW+   +   +  L+Y E+ N GPGAN   RV W GF  I  +  A +FTV 
Sbjct: 453 IDDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVG 512

Query: 319 KFINGTDWLPETGVPFKPGF 335
            FI+G+ WL  TG+PF  GF
Sbjct: 513 PFIDGSTWLNSTGIPFSLGF 532

BLAST of Cp4.1LG15g06250 vs. ExPASy Swiss-Prot
Match: O48711 (Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PME12 PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 5.9e-74
Identity = 135/308 (43.83%), Postives = 191/308 (62.01%), Query Frame = 0

Query: 29  IVALDGSGHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFIALIGDNAFTT 88
           +VA DG+G++  I++AI+ AP+ S  R  I+V  G+Y E+I IP  K  I LIGD +  T
Sbjct: 239 VVAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSDVT 298

Query: 89  IIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQADYTAYYKC 148
            I  NRS G G++T  SATL V G  F+A+ +   N+AG + H+AVA+   AD+ A Y+C
Sbjct: 299 FITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVALYRC 358

Query: 149 RFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYARFSK--NEIVVTAQS 208
              G+QDTLY      F +ECDIYGT+D+IFG+  V+FQ CNI ++        V+TAQS
Sbjct: 359 VIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVITAQS 418

Query: 209 KDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESFLDDNVQPKGW 268
           +D     +G S QNC +  S ++  +  K+K YLGRPWR++S  V MES++D+ +   GW
Sbjct: 419 RDTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFIDGSGW 478

Query: 269 MFWQ-GVPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRKKLAKKFTVEKFINGTDWLPE 328
             W  G  ++ L+Y E+NN GPG+ T  RV+W GFH++  + A  FT  +FI G  WL  
Sbjct: 479 SKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIMGYEDAFNFTATEFITGDGWLGS 538

Query: 329 TGVPFKPG 334
           T  P+  G
Sbjct: 539 TSFPYDNG 546

BLAST of Cp4.1LG15g06250 vs. NCBI nr
Match: KAG6572150.1 (putative pectinesterase/pectinesterase inhibitor 40, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 544 bits (1401), Expect = 2.54e-188
Identity = 264/297 (88.89%), Postives = 277/297 (93.27%), Query Frame = 0

Query: 1   MASFSFVACFFVCFINFLACVDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHV 60
           MASFSFVACFFVCFINFLACVDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHV
Sbjct: 1   MASFSFVACFFVCFINFLACVDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHV 60

Query: 61  TPGIYREHIQIPSTKKFIALIGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYL 120
           TPGIYREHIQIPSTKKFIALIGDNAFTTIIVDNRSNGTGFSTSDSAT+EVKGNNF+A++L
Sbjct: 61  TPGIYREHIQIPSTKKFIALIGDNAFTTIIVDNRSNGTGFSTSDSATMEVKGNNFIARHL 120

Query: 121 SFENSAGSQNHRAVAVLDQADYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFG 180
           SFENSAG QNH+AVAVLD A YTAYYKCRFLGFQDTLYLKG NNF KECDIYGTVDFIFG
Sbjct: 121 SFENSAGPQNHQAVAVLDNAKYTAYYKCRFLGFQDTLYLKGTNNFFKECDIYGTVDFIFG 180

Query: 181 SGLVMFQDCNIYARFSKNEIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYL 240
            GL MFQDCNIYAR S+NEIVVTAQSK++ M  SGF FQNC VTVS EI  N+AKIKIYL
Sbjct: 181 HGLAMFQDCNIYARLSRNEIVVTAQSKEKSMVHSGFCFQNCTVTVSPEIRANKAKIKIYL 240

Query: 241 GRPWRQYSSVVFMESFLDDNVQPKGWMFWQGVPVNKLFYAEFNNRGPGANTKHRVHW 297
           GRPWRQYSSVVFMESFLD+NVQP+GW FWQGVP+N LFYAEFNN G GANTKHRV+W
Sbjct: 241 GRPWRQYSSVVFMESFLDNNVQPEGWRFWQGVPINHLFYAEFNNGGLGANTKHRVNW 297

BLAST of Cp4.1LG15g06250 vs. NCBI nr
Match: XP_022952744.1 (pectinesterase 2-like [Cucurbita moschata])

HSP 1 Score: 501 bits (1289), Expect = 6.88e-176
Identity = 238/333 (71.47%), Postives = 277/333 (83.18%), Query Frame = 0

Query: 1   MASFSFVACFFVCFINFLACVDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHV 60
           MA  SF AC F  F N LA VD +RFNA+V+LDGSG +K IS+AIAAAPDNS TRFYI V
Sbjct: 1   MAFLSFAACLFFFFANILAHVDHIRFNAVVSLDGSGDFKSISEAIAAAPDNSNTRFYIRV 60

Query: 61  TPGIYREHIQIPSTKKFIALIGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYL 120
           +PG YR H++IP  KKFIALIGDNA TTIIVDNRS+GTGFST+DSATL V G++++A++L
Sbjct: 61  SPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVNGDHYMAQHL 120

Query: 121 SFENSAGSQNHRAVAVLDQADYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFG 180
           SFENSA  QN +AVAVL+ + +T YYKCRFL FQDTLYLKG   F +ECDIYGTVDFIFG
Sbjct: 121 SFENSAEHQNGQAVAVLNHSKFTTYYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFG 180

Query: 181 SGLVMFQDCNIYARFSKNEIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYL 240
           +GL MFQDCNIYAR   NEI VTAQSK+Q   KSGFSFQNC VTVS +I   +  +KI+L
Sbjct: 181 NGLAMFQDCNIYARLQSNEITVTAQSKEQSWVKSGFSFQNCTVTVSPDIASRKVDVKIFL 240

Query: 241 GRPWRQYSSVVFMESFLDDNVQPKGWMFWQGVPVNKLFYAEFNNRGPGANTKHRVHWSGF 300
           GRPWRQYS+VVFMESFLDDNVQP+GW+ W+GVP+N LFYAEFNNRGPGA+T HRV+W+ F
Sbjct: 241 GRPWRQYSTVVFMESFLDDNVQPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNWTSF 300

Query: 301 HVIRKKLAKKFTVEKFINGTDWLPETGVPFKPG 333
           HV+ K+LAK FTVE FING+DWLPET VPF+ G
Sbjct: 301 HVLDKQLAKNFTVENFINGSDWLPETHVPFRTG 333

BLAST of Cp4.1LG15g06250 vs. NCBI nr
Match: KAG6572387.1 (putative pectinesterase/pectinesterase inhibitor 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 500 bits (1287), Expect = 1.39e-175
Identity = 239/333 (71.77%), Postives = 275/333 (82.58%), Query Frame = 0

Query: 1   MASFSFVACFFVCFINFLACVDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHV 60
           MA     AC F  F N LA VDS+RFNA+V+LDGSG +K IS+AIAAAP+NS TRFYI V
Sbjct: 1   MAFLQLAACLFFFFANVLALVDSIRFNAVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRV 60

Query: 61  TPGIYREHIQIPSTKKFIALIGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYL 120
           TPGIYR H++IP  KKFIALIGDNA TTIIVDNRS+GTGF TS+SATL V G+NF+A++L
Sbjct: 61  TPGIYRGHLEIPPAKKFIALIGDNALTTIIVDNRSHGTGFKTSESATLVVNGDNFMAQHL 120

Query: 121 SFENSAGSQNHRAVAVLDQADYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFG 180
           SFENSA  QN +AVAVLD A +T YY CRF+ FQDTLY KG   F +ECDIYGTVDFIFG
Sbjct: 121 SFENSAEPQNGQAVAVLDYAKHTVYYNCRFMSFQDTLYSKGEFQFFRECDIYGTVDFIFG 180

Query: 181 SGLVMFQDCNIYARFSKNEIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYL 240
           +GL +FQDCNIYAR   +EI VTAQSK+Q + +SGFSFQNC VTVS EI  N+ K+KIYL
Sbjct: 181 NGLAVFQDCNIYARLPMDEITVTAQSKEQSVSQSGFSFQNCTVTVSPEITANKTKVKIYL 240

Query: 241 GRPWRQYSSVVFMESFLDDNVQPKGWMFWQGVPVNKLFYAEFNNRGPGANTKHRVHWSGF 300
           GRPWR+YS+VVFMESFLDDNVQP+GW+ W+GVP+N LFYAEFNNRGPGANT HRV+W  F
Sbjct: 241 GRPWREYSTVVFMESFLDDNVQPQGWISWEGVPINNLFYAEFNNRGPGANTTHRVNWPTF 300

Query: 301 HVIRKKLAKKFTVEKFINGTDWLPETGVPFKPG 333
           HV+ K LAK FTVE FING+DWLPET VPF+ G
Sbjct: 301 HVLDKLLAKNFTVENFINGSDWLPETHVPFRTG 333

BLAST of Cp4.1LG15g06250 vs. NCBI nr
Match: XP_023511662.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 499 bits (1286), Expect = 1.97e-175
Identity = 238/333 (71.47%), Postives = 276/333 (82.88%), Query Frame = 0

Query: 1   MASFSFVACFFVCFINFLACVDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHV 60
           MA   F AC F  F N LA +D  RFNA+V+LDGSG +K IS+AIAAAPDNS TRFYI V
Sbjct: 1   MAFLPFAACLFFFFANVLALIDPARFNAVVSLDGSGDFKSISEAIAAAPDNSNTRFYIRV 60

Query: 61  TPGIYREHIQIPSTKKFIALIGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYL 120
           TPG YR H++IP  KKFIALIGDNA TTIIVDNRS+GTGFST+DSATL V+G+NF+A++L
Sbjct: 61  TPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVRGHNFMAQHL 120

Query: 121 SFENSAGSQNHRAVAVLDQADYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFG 180
           SFENSAG QN +AVAVLD A YT YY CRFL FQDTLY +G   F +EC++YGTVDFIFG
Sbjct: 121 SFENSAGPQNGQAVAVLDYAKYTVYYDCRFLSFQDTLYSRGQCQFFRECEVYGTVDFIFG 180

Query: 181 SGLVMFQDCNIYARFSKNEIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYL 240
            GL +FQDCNIYAR   +EI VTAQSKD+   KSGFSFQNC VTVS EI  ++ K+KIYL
Sbjct: 181 DGLAVFQDCNIYARLPSDEITVTAQSKDRSWWKSGFSFQNCTVTVSPEIASSKDKVKIYL 240

Query: 241 GRPWRQYSSVVFMESFLDDNVQPKGWMFWQGVPVNKLFYAEFNNRGPGANTKHRVHWSGF 300
           GRPWRQYS+VVFMESFLDDNV+P+GW+ W+GVP+N LFYAEFNNRGPGA+T HRV+WS F
Sbjct: 241 GRPWRQYSTVVFMESFLDDNVRPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNWSTF 300

Query: 301 HVIRKKLAKKFTVEKFINGTDWLPETGVPFKPG 333
           HV+ K+LAK FTV+ FING+DWLPET VPF+ G
Sbjct: 301 HVLDKQLAKNFTVKFFINGSDWLPETHVPFRTG 333

BLAST of Cp4.1LG15g06250 vs. NCBI nr
Match: XP_022952377.1 (probable pectinesterase/pectinesterase inhibitor 7 isoform X1 [Cucurbita moschata])

HSP 1 Score: 498 bits (1281), Expect = 1.10e-174
Identity = 240/335 (71.64%), Postives = 274/335 (81.79%), Query Frame = 0

Query: 1   MASFSFVACFFVCFINFLACVDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHV 60
           MA F  ++CFF+CF+NFLA VDS  FNA VALDG+G++K I+DAIAAAP+NS TRFYI V
Sbjct: 1   MAFFQRISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV 60

Query: 61  TPGIYREHIQIPSTKKFIALIGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYL 120
           +PGIY EH++I S KKFIALIG+N FTTIIVDNRSN  GF TS+SAT+ VKGNNF+A++L
Sbjct: 61  SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL 120

Query: 121 SFENSAGSQNHRAVAVLDQADYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFG 180
           SFENSA  Q+ +AVAVLD A YT YYKCRFL FQDTLY KG   F K CDIYGTVDFIFG
Sbjct: 121 SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG 180

Query: 181 SGLVMFQDCNIYARFSKNEIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYL 240
           +GL MFQDCN+YAR   N+  VTAQSK Q   +SGFSFQNC VTVS EI   +A +KIYL
Sbjct: 181 NGLAMFQDCNVYARLPSNDSTVTAQSKKQ-WTQSGFSFQNCTVTVSPEIASCKALVKIYL 240

Query: 241 GRPWRQYSSVVFMESFLDDNVQPKGWMFWQGVPVNKLFYAEFNNRGPGANTKHRVHWSGF 300
           GRPW+ YS+VVFMESFLDDNVQP+GWM W GVPVN LFYAE+NNRGPGANT HRV+W GF
Sbjct: 241 GRPWKPYSTVVFMESFLDDNVQPQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPGF 300

Query: 301 HVIRKKLAKKFTVEKFINGTDWLPETGVPFKPGFY 335
           HVI ++LAK FTVE FINGT WLPET VP+  G Y
Sbjct: 301 HVIDRQLAKSFTVENFINGTYWLPETNVPYTSGLY 334

BLAST of Cp4.1LG15g06250 vs. ExPASy TrEMBL
Match: A0A6J1GMM0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455349 PE=4 SV=1)

HSP 1 Score: 501 bits (1289), Expect = 3.33e-176
Identity = 238/333 (71.47%), Postives = 277/333 (83.18%), Query Frame = 0

Query: 1   MASFSFVACFFVCFINFLACVDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHV 60
           MA  SF AC F  F N LA VD +RFNA+V+LDGSG +K IS+AIAAAPDNS TRFYI V
Sbjct: 1   MAFLSFAACLFFFFANILAHVDHIRFNAVVSLDGSGDFKSISEAIAAAPDNSNTRFYIRV 60

Query: 61  TPGIYREHIQIPSTKKFIALIGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYL 120
           +PG YR H++IP  KKFIALIGDNA TTIIVDNRS+GTGFST+DSATL V G++++A++L
Sbjct: 61  SPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVNGDHYMAQHL 120

Query: 121 SFENSAGSQNHRAVAVLDQADYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFG 180
           SFENSA  QN +AVAVL+ + +T YYKCRFL FQDTLYLKG   F +ECDIYGTVDFIFG
Sbjct: 121 SFENSAEHQNGQAVAVLNHSKFTTYYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIFG 180

Query: 181 SGLVMFQDCNIYARFSKNEIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYL 240
           +GL MFQDCNIYAR   NEI VTAQSK+Q   KSGFSFQNC VTVS +I   +  +KI+L
Sbjct: 181 NGLAMFQDCNIYARLQSNEITVTAQSKEQSWVKSGFSFQNCTVTVSPDIASRKVDVKIFL 240

Query: 241 GRPWRQYSSVVFMESFLDDNVQPKGWMFWQGVPVNKLFYAEFNNRGPGANTKHRVHWSGF 300
           GRPWRQYS+VVFMESFLDDNVQP+GW+ W+GVP+N LFYAEFNNRGPGA+T HRV+W+ F
Sbjct: 241 GRPWRQYSTVVFMESFLDDNVQPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNWTSF 300

Query: 301 HVIRKKLAKKFTVEKFINGTDWLPETGVPFKPG 333
           HV+ K+LAK FTVE FING+DWLPET VPF+ G
Sbjct: 301 HVLDKQLAKNFTVENFINGSDWLPETHVPFRTG 333

BLAST of Cp4.1LG15g06250 vs. ExPASy TrEMBL
Match: A0A6J1GK92 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455081 PE=4 SV=1)

HSP 1 Score: 498 bits (1281), Expect = 5.30e-175
Identity = 240/335 (71.64%), Postives = 274/335 (81.79%), Query Frame = 0

Query: 1   MASFSFVACFFVCFINFLACVDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHV 60
           MA F  ++CFF+CF+NFLA VDS  FNA VALDG+G++K I+DAIAAAP+NS TRFYI V
Sbjct: 1   MAFFQRISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV 60

Query: 61  TPGIYREHIQIPSTKKFIALIGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYL 120
           +PGIY EH++I S KKFIALIG+N FTTIIVDNRSN  GF TS+SAT+ VKGNNF+A++L
Sbjct: 61  SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL 120

Query: 121 SFENSAGSQNHRAVAVLDQADYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFG 180
           SFENSA  Q+ +AVAVLD A YT YYKCRFL FQDTLY KG   F K CDIYGTVDFIFG
Sbjct: 121 SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG 180

Query: 181 SGLVMFQDCNIYARFSKNEIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYL 240
           +GL MFQDCN+YAR   N+  VTAQSK Q   +SGFSFQNC VTVS EI   +A +KIYL
Sbjct: 181 NGLAMFQDCNVYARLPSNDSTVTAQSKKQ-WTQSGFSFQNCTVTVSPEIASCKALVKIYL 240

Query: 241 GRPWRQYSSVVFMESFLDDNVQPKGWMFWQGVPVNKLFYAEFNNRGPGANTKHRVHWSGF 300
           GRPW+ YS+VVFMESFLDDNVQP+GWM W GVPVN LFYAE+NNRGPGANT HRV+W GF
Sbjct: 241 GRPWKPYSTVVFMESFLDDNVQPQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPGF 300

Query: 301 HVIRKKLAKKFTVEKFINGTDWLPETGVPFKPGFY 335
           HVI ++LAK FTVE FINGT WLPET VP+  G Y
Sbjct: 301 HVIDRQLAKSFTVENFINGTYWLPETNVPYTSGLY 334

BLAST of Cp4.1LG15g06250 vs. ExPASy TrEMBL
Match: A0A6J1GM54 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455508 PE=4 SV=1)

HSP 1 Score: 496 bits (1277), Expect = 2.23e-174
Identity = 237/333 (71.17%), Postives = 275/333 (82.58%), Query Frame = 0

Query: 1   MASFSFVACFFVCFINFLACVDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHV 60
           MA     AC F  F N LA VDS+RFNA+V+LDGSG +K IS+AIAAAP+NS TRFYI V
Sbjct: 1   MAFLQLAACLFFFFANVLALVDSIRFNAVVSLDGSGDFKSISEAIAAAPNNSNTRFYIRV 60

Query: 61  TPGIYREHIQIPSTKKFIALIGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYL 120
           TPGIYR H++IP  KKFIALIGD+A TTIIVDNRS+GTGF TS+SATL V G+NF+A++L
Sbjct: 61  TPGIYRGHLEIPPAKKFIALIGDSALTTIIVDNRSHGTGFKTSESATLVVNGDNFMAQHL 120

Query: 121 SFENSAGSQNHRAVAVLDQADYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFG 180
           SFENSA  QN +AVAVLD A ++ YY CRF+ FQDTLY KG   F +ECDIYGTVDFIFG
Sbjct: 121 SFENSAEPQNGQAVAVLDYAKHSVYYDCRFMSFQDTLYSKGEFQFFRECDIYGTVDFIFG 180

Query: 181 SGLVMFQDCNIYARFSKNEIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYL 240
           +GL +FQDCNIYAR   +EI VTAQSK+Q + +SGFSFQNC VTVS EI  N+ K+KIYL
Sbjct: 181 NGLAVFQDCNIYARLPMDEITVTAQSKEQSVSQSGFSFQNCTVTVSPEITANKTKVKIYL 240

Query: 241 GRPWRQYSSVVFMESFLDDNVQPKGWMFWQGVPVNKLFYAEFNNRGPGANTKHRVHWSGF 300
           GRPWR+YS+VVFMESFLDDNVQP+GW+ W+GVP+N LFYAEFNNRGPGANT HRV+W  F
Sbjct: 241 GRPWREYSTVVFMESFLDDNVQPQGWISWEGVPINNLFYAEFNNRGPGANTTHRVNWPTF 300

Query: 301 HVIRKKLAKKFTVEKFINGTDWLPETGVPFKPG 333
           HV+ K LAK FTVE FING+DWLPET VPF+ G
Sbjct: 301 HVLDKLLAKNFTVENFINGSDWLPETHVPFRTG 333

BLAST of Cp4.1LG15g06250 vs. ExPASy TrEMBL
Match: A0A6J1GLJ0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455081 PE=4 SV=1)

HSP 1 Score: 488 bits (1257), Expect = 1.79e-171
Identity = 236/326 (72.39%), Postives = 269/326 (82.52%), Query Frame = 0

Query: 1   MASFSFVACFFVCFINFLACVDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHV 60
           MA F  ++CFF+CF+NFLA VDS  FNA VALDG+G++K I+DAIAAAP+NS TRFYI V
Sbjct: 1   MAFFQRISCFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIRV 60

Query: 61  TPGIYREHIQIPSTKKFIALIGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYL 120
           +PGIY EH++I S KKFIALIG+N FTTIIVDNRSN  GF TS+SAT+ VKGNNF+A++L
Sbjct: 61  SPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQHL 120

Query: 121 SFENSAGSQNHRAVAVLDQADYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFG 180
           SFENSA  Q+ +AVAVLD A YT YYKCRFL FQDTLY KG   F K CDIYGTVDFIFG
Sbjct: 121 SFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIFG 180

Query: 181 SGLVMFQDCNIYARFSKNEIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYL 240
           +GL MFQDCN+YAR   N+  VTAQSK Q   +SGFSFQNC VTVS EI   +A +KIYL
Sbjct: 181 NGLAMFQDCNVYARLPSNDSTVTAQSKKQ-WTQSGFSFQNCTVTVSPEIASCKALVKIYL 240

Query: 241 GRPWRQYSSVVFMESFLDDNVQPKGWMFWQGVPVNKLFYAEFNNRGPGANTKHRVHWSGF 300
           GRPW+ YS+VVFMESFLDDNVQP+GWM W GVPVN LFYAE+NNRGPGANT HRV+W GF
Sbjct: 241 GRPWKPYSTVVFMESFLDDNVQPQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPGF 300

Query: 301 HVIRKKLAKKFTVEKFINGTDWLPET 326
           HVI ++LAK FTVE FINGT WLPET
Sbjct: 301 HVIDRQLAKSFTVENFINGTYWLPET 325

BLAST of Cp4.1LG15g06250 vs. ExPASy TrEMBL
Match: A0A5D3BKF8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004210 PE=4 SV=1)

HSP 1 Score: 473 bits (1218), Expect = 2.12e-165
Identity = 229/324 (70.68%), Postives = 261/324 (80.56%), Query Frame = 0

Query: 13  CFINFLACVDSVRFNAIVALDGS-GHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQI 72
           CF N +  + S  FNA V+LDGS  H+K I+ AIAAAPDNS TRFYI VTPG Y E +QI
Sbjct: 13  CFFNLVPFIHSFTFNATVSLDGSMNHFKTIAAAIAAAPDNSNTRFYIRVTPGTYHERLQI 72

Query: 73  PSTKKFIALIGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNH 132
           P TK FIALIGDNA TTIIVD+RSN  GF TSDSATL V GNNF+AK L+FENSAG QN 
Sbjct: 73  PPTKTFIALIGDNALTTIIVDDRSNARGFKTSDSATLTVNGNNFLAKSLTFENSAGPQNG 132

Query: 133 RAVAVLDQADYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNI 192
           +AVAVLDQA +TAY+KC+FLGFQDTLY+KG   F KEC+IYG+VDFIFG+GLVMFQDCNI
Sbjct: 133 QAVAVLDQAHFTAYHKCKFLGFQDTLYVKG-TQFFKECEIYGSVDFIFGNGLVMFQDCNI 192

Query: 193 YARFSKNEIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVV 252
           YAR     I VTAQSK+QP   SGFSFQNC VTVSSEI VN+A +KIYLGRPWRQYS VV
Sbjct: 193 YAREPNRPITVTAQSKEQPKEVSGFSFQNCTVTVSSEIAVNKANVKIYLGRPWRQYSKVV 252

Query: 253 FMESFLDDNVQPKGWMFWQGVPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRKKLAKKF 312
           FMESFLDD V P+GW  W GVPVN LFYAEFNNRGPGA+   RVHW+GF V+ K+ A +F
Sbjct: 253 FMESFLDDEVVPEGWSEWPGVPVNNLFYAEFNNRGPGADVSKRVHWTGFRVLDKQSAYQF 312

Query: 313 TVEKFINGTDWLPETGVPFKPGFY 335
           TV+ F+NG+ WLPETG+PF+ G +
Sbjct: 313 TVDNFVNGSKWLPETGIPFRDGLF 335

BLAST of Cp4.1LG15g06250 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 283.9 bits (725), Expect = 1.7e-76
Identity = 135/310 (43.55%), Postives = 196/310 (63.23%), Query Frame = 0

Query: 30  VALDGSGHYKRISDAIAAAPDN---SKTRFYIHVTPGIYREHIQIPSTKKFIALIGDNAF 89
           V+ DG+G++  I+ A+AAAP+N   S   F I+VT GIY E+I I   K+++ +IGD   
Sbjct: 267 VSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGIN 326

Query: 90  TTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQADYTAYY 149
            T++  NRS   G++T +SAT  V   NFVA  ++F N+AG + H+AVA+   AD++ +Y
Sbjct: 327 QTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSIFY 386

Query: 150 KCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYAR--FSKNEIVVTA 209
            C F  +QDTLY      F +ECD+YGTVDFIFG+  V+FQ+CN+Y R         +TA
Sbjct: 387 SCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAITA 446

Query: 210 QSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESFLDDNVQPK 269
           Q +  P + +G S QNC +  + ++  +   +K YLGRPW++YS  V+M+S++D  V+P 
Sbjct: 447 QGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPV 506

Query: 270 GWMFWQG-VPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRKKLAKKFTVEKFINGTDWL 329
           GW  W G   ++ L+YAE+NN GPG+NT +RV W G+HVI    A  FTV       DW+
Sbjct: 507 GWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANFTVTGLFIEADWI 566

Query: 330 PETGVPFKPG 334
            +TGVP+  G
Sbjct: 567 WKTGVPYTSG 576

BLAST of Cp4.1LG15g06250 vs. TAIR 10
Match: AT4G02320.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 281.6 bits (719), Expect = 8.5e-76
Identity = 138/314 (43.95%), Postives = 199/314 (63.38%), Query Frame = 0

Query: 21  VDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFIAL 80
           VD  + N +VA +G+G+Y  I +AI+AAP++S+TRF I++  G Y E+I+IP  K  I  
Sbjct: 201 VDETKVNLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMF 260

Query: 81  IGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQA 140
           IGD    T+I  NRS   G++   SAT+ V+G+ F+AK LSF N AG + H+AVA+   +
Sbjct: 261 IGDGIGRTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVALRSSS 320

Query: 141 DYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYAR--FSKN 200
           D +AYY+C F  +QDT+Y+  H  F +ECDIYGTVDFIFG   V+FQ+C++YAR      
Sbjct: 321 DLSAYYRCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQ 380

Query: 201 EIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESFLD 260
           +I+ TAQ ++     +G S  +  +  + ++   +A  K YLGRPW+ YS  V M+SF+D
Sbjct: 381 KIIYTAQGRENSREPTGISIISSRILAAPDLIPVQANFKAYLGRPWQLYSRTVIMKSFID 440

Query: 261 DNVQPKGWMFWQ-GVPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRK-KLAKKFTVEKF 320
           D V P GW+ W+    +  L+Y E+ N GPG+N  +RV W GF  I   + A +F+V  F
Sbjct: 441 DLVDPAGWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPF 500

Query: 321 INGTDWLPETGVPF 331
           I+G  WL  T +PF
Sbjct: 501 IDGNKWLNSTRIPF 514

BLAST of Cp4.1LG15g06250 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 279.6 bits (714), Expect = 3.2e-75
Identity = 139/320 (43.44%), Postives = 202/320 (63.12%), Query Frame = 0

Query: 19  ACVDSVRFNAIVALDGSGHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFI 78
           A V    +N  VA+DG+G++  I+DA+ AAP+ S+TRF I++  G Y E++++P  K  I
Sbjct: 213 APVQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMI 272

Query: 79  ALIGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLD 138
             IGD    T+I  NRS   G+ST  + T+ VKG  ++AK +SF NSAG    +AVA   
Sbjct: 273 MFIGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRS 332

Query: 139 QADYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYARFSK- 198
            +D++A+Y+C F G+QDTLY+     F +ECDIYGT+DFIFG+  V+FQ+ ++YAR    
Sbjct: 333 GSDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNP 392

Query: 199 -NEIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESF 258
            ++I  TAQS++Q  + +G S  NC +  + ++   +   K YLGRPWR+YS  V ++SF
Sbjct: 393 GHKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSF 452

Query: 259 LDDNVQPKGWMFW-QGVPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRKKL-AKKFTVE 318
           +DD + P GW+   +   +  L+Y E+ N GPGAN   RV W GF  I  +  A +FTV 
Sbjct: 453 IDDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVG 512

Query: 319 KFINGTDWLPETGVPFKPGF 335
            FI+G+ WL  TG+PF  GF
Sbjct: 513 PFIDGSTWLNSTGIPFSLGF 532

BLAST of Cp4.1LG15g06250 vs. TAIR 10
Match: AT2G26440.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 279.3 bits (713), Expect = 4.2e-75
Identity = 135/308 (43.83%), Postives = 191/308 (62.01%), Query Frame = 0

Query: 29  IVALDGSGHYKRISDAIAAAPDNSKTRFYIHVTPGIYREHIQIPSTKKFIALIGDNAFTT 88
           +VA DG+G++  I++AI+ AP+ S  R  I+V  G+Y E+I IP  K  I LIGD +  T
Sbjct: 239 VVAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSDVT 298

Query: 89  IIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVAVLDQADYTAYYKC 148
            I  NRS G G++T  SATL V G  F+A+ +   N+AG + H+AVA+   AD+ A Y+C
Sbjct: 299 FITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVALYRC 358

Query: 149 RFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYARFSK--NEIVVTAQS 208
              G+QDTLY      F +ECDIYGT+D+IFG+  V+FQ CNI ++        V+TAQS
Sbjct: 359 VIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVITAQS 418

Query: 209 KDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFMESFLDDNVQPKGW 268
           +D     +G S QNC +  S ++  +  K+K YLGRPWR++S  V MES++D+ +   GW
Sbjct: 419 RDTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFIDGSGW 478

Query: 269 MFWQ-GVPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRKKLAKKFTVEKFINGTDWLPE 328
             W  G  ++ L+Y E+NN GPG+ T  RV+W GFH++  + A  FT  +FI G  WL  
Sbjct: 479 SKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIMGYEDAFNFTATEFITGDGWLGS 538

Query: 329 TGVPFKPG 334
           T  P+  G
Sbjct: 539 TSFPYDNG 546

BLAST of Cp4.1LG15g06250 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 277.3 bits (708), Expect = 1.6e-74
Identity = 132/321 (41.12%), Postives = 201/321 (62.62%), Query Frame = 0

Query: 21  VDSVRFNAIVAL--DGSGHYKRISDAIAAAP---DNSKTRFYIHVTPGIYREHIQIPSTK 80
           V++V  + IV +  +G+G++  I++A+ +AP   D +   F I+VT G+Y E++ I   K
Sbjct: 250 VNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNK 309

Query: 81  KFIALIGDNAFTTIIVDNRSNGTGFSTSDSATLEVKGNNFVAKYLSFENSAGSQNHRAVA 140
           +++ +IGD    T++  NR+   G++T +SAT  V   NFVA  ++F N+AG + H+AVA
Sbjct: 310 RYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVA 369

Query: 141 VLDQADYTAYYKCRFLGFQDTLYLKGHNNFLKECDIYGTVDFIFGSGLVMFQDCNIYAR- 200
           +   AD + +Y C F  +QDTLY      F +ECDIYGTVDFIFG+  V+FQDCN+Y R 
Sbjct: 370 MRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQ 429

Query: 201 -FSKNEIVVTAQSKDQPMRKSGFSFQNCIVTVSSEIGVNEAKIKIYLGRPWRQYSSVVFM 260
                   +TAQ +  P + +G S  NC +  + ++  +   +K YLGRPW++YS  VFM
Sbjct: 430 PMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFM 489

Query: 261 ESFLDDNVQPKGWMFWQG-VPVNKLFYAEFNNRGPGANTKHRVHWSGFHVIRKKLAKKFT 320
           +S++D+ V+P GW  W G   ++ L+YAE+NN G G++T  RV W G+HVI    A  FT
Sbjct: 490 QSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFT 549

Query: 321 VEKFINGTDWLPETGVPFKPG 334
           VE F+ G  W+ ++GVP+  G
Sbjct: 550 VENFLLGDGWMVQSGVPYISG 570