Cp4.1LG15g04680 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG15g04680
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionLipase
LocationCp4.1LG15: 5674271 .. 5679736 (+)
RNA-Seq ExpressionCp4.1LG15g04680
SyntenyCp4.1LG15g04680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTCGCGTCTAATTTGTCTCGCTGTTACTTTGATGGTCAATCTTGTTCCTGAAGATGGCGAAGCCATTGATGGCTATGGTATTTCTTCTTTGCTTCTTCGCTCTCCATTCTGCCGATGATTCCTCTTATCGTTCCCTTGAGTACTCGATTCTCCCTCGCGCTCTGTCTGACCAGAAGTCCTTGTGCTCTCAACTTGTTCAACCTGTTGGCTATCCTTGCGCGGAGCATCAGGTACTGTGCTTCTCTCATATCGGTTCTGATTTTTTGGTTGATTGATTAGTGGAAGTAGTGGATTAGGAATTGTTTGTGGATGTTAGTGTTCATTGCTCTGTTTTGACTGGAGAAACTGGAGTGGATTTAGTCAACTAATTGGCTCTAACGCTTCTTGTTTGAGAGTTTTTTTTTTCCTCTCTCTCCTGAGGTGAGAATGATGGAATGATTTTTCATTTAATTCTTAGATTCGATGTCCGTCCTGGTTTGATTTGTTTCTGTCGAGTTTCTGGATAGTCCGATGCGTTTGTATTGTTCATTGTGTTCATGATGGAAAGTATGAGTACAGAGATAATACGCGAAGGAAAATGACCGTATTGGCTTGATTTTATCGAATTTTCATTAGCTATTCAGAGGCTCAAGCATTTGTAGCGTATGTTGGATGATGAAAGTCCCACAACTAATTTAGGGAACGACCCTGAGTTTATAACCAAGGAATACTATCTCCATTGGTATGAGGCCTTTTGGGGAAGCCCAAAGCAAAACCATGGGAGCTTATGCTCAAAGTGGAAATATCATACCATTGTGGAGAGTCGTGTTCGTTGCGTATACGTATGCACTTTTAAATAGTATCTTCTTTTTATCACTCTCTTGAATTCTGAGGAATGCTATTCATTATTGCAGACTGGGATGATGAATTGTGGTTTTAGGGGCGCACTTGATTACGTTTGTGCATTACTGACGGACGTGATTTTGTTATAAATTTCTTAGATACAAACGAAGGATGGATTTTTATTGGGTCTTCAACGAGTATCTTCACGCGATGGAGAATTGGAAAAGCAAAAGGGCCCTCCAATTTTGCTACTCCATGGACTGTTCATGGTGAGTCTTCCTACAGTTTTGCAACTGTATTTGTAAAATTACTTTACTCTTTATGGTTCAACCCTATCAGCGATAGTTAGAAAGTTTTCAAGGTTGCCTGATATTTTTATTATACTATTGAGACTTATCTCGTTTCTTTGAAATTTTCAACTGCATCATTTCTACAAATTCCAGTAAAATGATTAGTTTTCAAGAAAAAGTAATTATGGTTTTCTTTTAGGTCAGTATTAACCTTAAACTTCTACAGTCCAATTGTTCAAACATGCCTTTGAGTTCGCTTAAGGAATGGTATTGTAAGATGAATGACATAAAGGCTCCTGGAAAATTTTTAGCCAAGAAAGTATCTTATACTTCTGTCTTTCTNTTTCTTCTTCCTCCTTCCCCTTGGTATTCTATTTTTTCTGTTTGCTGCACATTTCAAAGCATTTTGAAACATGGATTGTGAATTTTGAATAATCTGTAGACTGGGATCATTCTTACAGATAGGAGTTTCAACTACTAGCTTTTTATTTCTTTCATTGCCTGAACTGGTGCTCGTGCAGGGTGGCGATGCATGGTTTTTGAATTCTGCTAGAGAATCTTTAGGATTTATCCTTGCAGATAATGGATTTGATGTGTGGATAGGAAATGTGCGTGGAACTCGGTGGAGTCATGGGCATACATCCGTATCAGAGGATGAGAAGGTACTTATATTTCTTTATTATAATATGCCTAAGGTGGGTAAGATGCTAATTAAATGCAGAGTAAGAACATATTAATAGACAATTTCTTCATTGTGTTATGTGTATATTGGATGTATCTAGAAAGTTATATAACATACAAACATGCTGCTGCTCATCCATGCTCAACGCGCTGATCAATGTGTTGAGGTGGAGAGTATCATACAGTTAATTTTGGAGGTTCAAACAAATTTGAGTGAGTTTTTTCGGAGTTTTGCAGTTACATGACAGATTATATTTAAGAACCATTTGTTTCATGCCAACTGTGTTCATTTGCTAAAGCCTATTTTGTGTCGAATCTCTGCACTATATTGACAGTAATGTAACATAAGTTGAATATGTTTAGTTTCTGGTTATGTCTTTGACTCTAGCATATTCTGATTGTAGGAGTTTTGGGATTGGAGTTGGGAAGACTTGGCTTTGTATGATCTTGCAGGCATGATTGACTACATAAATTCATTGACAAAGAGGAAAATTTATGTTGTTGGGCACTCACAGGTCTCATATACCCTCTTTTATAAGATTCTTTGTTGAGGATTGTTGTGAGGGATCCCAAGTTGGGCTAGAGTTTATAAGTAAGGAATACGTTTTCATTGGTATGAGGCCTTTTGGGGAAGCCCAAAGCAAAGCCATGAGAGCTTATAAAGGTGTGGAAACTTTCCCCTAGCAGACGCGTTTTAAAGCCTTGAGGGGAAGCCCGAAAGGGAAAGCCCAAAGAGGACAATATCTGCTAGCGGTGGATCTGGGCCGTTATAAATAGTATCAGAGCCAGACACCGGACGATGTGCTAGCCTTCTCATTGTTCCCCGAAGAGGGGTAGACACGAGACGGTGTGCCAGCAAGGACGCTGGGCCCCAAAGGAGGGTGGATTTGGGGGCGGTCCCACATTAATGGGAGGAAGGGAAGAGTGCCAGCGAGGATGCTGGGCCCCGAAGGGGGGTGGATTGTGATGTCCCACATTGGTTGGGGAGGAGAACAAACCACCATTTATAAAGGTGTGGAAACCTTCTCCTAGCAGACGTGTTTTAAAGCCTTGAGGGGAAGCCCGAAAGGGAAAGCCCAAAGAGGACAATATCTGCCGGTGGATCTGGGCCGTTACATGTGTCATCTTTTGTCTTTTTTCGCAGGGAACAATTATGTCTTTTGCTGCTCTTACCCAGCCAGACATAGCCAAGAAAGTTGAAGCAGCTGCTCTTTTAAGTCCCATATCGTACTTGGAGCATATCACTGCTCCATTAGTACGTCTAATGGTTGACACACATCTCGATACGGTATTCCTGGCTTGTTATTGTGTAATTAGCATTGTTAAATGTATTATTACTAGTAATAAGCTTGTTCCTATATTTTTCTCGGCAGATAATTCTTGCCGCGGGATTTCATGAACTCAACTTTAAAAGGTTGTCATCCTATGCTATGTCCTATTTTGATTCTGTAATTGGTTGCATTTTTAGCTTCAGTATAATTCATGACACTAATGTCTATCTTTTGTACCCTACTTCAGCGATTGGGGAACAGTCCTTTTAGATGATCTTTGTGATCGGCTCGTTAACTGCATCAATGTTCTAAGTTCAATAACAGGTTCTCTAAATGCAAAGCGCATCATGTTTCTTTCTCTTTTCTTATTTTCCTTCTGTTTGGCTTCCAATCCAGATAGGAAATTCTTGCTACTTTTTAATTATTTAGTTAATTGTCTAGCAATTCAAGAAATGGACTACCTACTAGCTAAGCTTCCATTTTTTACTCTCATGCATATCTGCATTTTCATCCATTTTGTTGAACTTAACTTTATATACAGCTTTTTCAGAATCTAAATGCCCTTGGATGGGAAAATTTCAATTATGGGACTTAAAAAAATTTCATGATTCAATAATTATTGATGTCAGGATTGTCATGTTTCTTCTTGTAGTCGAAACAATTTGGTTACAACTACTCGATAATGAGTTTTTGATTGGAAAGACTACAAAATGTTGCTTTGTTCTTTGTAGGAAAGTATCAAGTTTGAATGTTCAATCTATTCAATTGCAGACGTTAAATTTCCTAACAAGGCCTTCTTATAATGTGGCAGGTGAAAATTGTTGCCTTAATGGCTCACGGTTCGACTTATTTTTCGAATACGAACCACATCCATCATCAGCAAAAAACTTGCACCATCTTTTCCAAAGTACGCTCTGCAGTATCCTCTTTAGCTTCATGTTCTATTATACTATGATTTCGATGAATCTATAAGTTTTTCACTTGCATATCTGTTGGGATGTTACTTTTAGTGTTCCCTTGTGAAGTCTAATATAAATCCTTAAACATTTGATTGTGAAACTTTGTGAGACTTCTCTAGGGAAGTTACTTTTAGTGTTCCCGTGAGACTACTCCATAAAGGGATTTCCATATGCTCTTTAATCTCTTTCCTGAGCGACTTTTCATCTCCAATAAATCCCTCGCTTTCCAGTTTTCATCAACTTTGTCTCTGATTCAAGCTACCCCTCCAGCTGCTCAAGAAATTCCAGAACCATCATCCCCTTATAAAAGCCCGAGGATCCTCCCATCCTTGATTGAATTTCTAGTACCTTCCCTTTCCACCTTCCCCTGTTCCTTTAAATCAAAGCTCCAATTTCTGCTTGATGTTTTAAAATCTTCTTATTTCCTTTCACGTTTGCTGGGTGCAAACAACATGACCAAATGTCAGATCCCACATTGGTTGGGGAAGAGAACGAAACACCCTTTATAAGAGTGTGGAAACCTCTCCCTAGCATACGTGTTTTAAAAACCTTGAGGGGAAGCTCGAAAGAGAAAGCCCAACAAGGACAATATCTATTAGCGGTGGGCTTGGACCGTGACACCAAACTTCGGTTATTATTCCAAGCTGTTTTCTAACGCGAGTCATCTTTGTCGTATCGTAATAAAGTGAGAACAGTACTAATGATTTAGGATCAGTTTTTCTGTTGTTAAATCTGTTGATACTTTAGAATTTGAAAGAAGAAGAATTAAGCAAGAATGTATTCTGACATCTATTTCAAAGATTTGGAGATAGTGCTATTTGTCTCTAACTGCCGTATTACCGAACTAACAAAACTGTTAGGAATCACAACTCTCTACGATGATAGAGCTTTTTCATGGCTTTGCTTTGGGTTTCCTCAATAGGTCTCGTATCAATGGAGATGTATTCCTTACTTATAAACTCATGATCAATCCTCAACAGAAACTTCTCCTTAAAAGAAAAACCGATCCTAAACCATCACTAATATCCTCACTGAAATTGAATGTTTTTCTCCTCCTGGAGGGAATGGGGAGGCTGTTCGATACAGCTGATTTAACTGCCTTCCTTTTCTTTTACCAGTGATCCGCAAGGGTACTTTTTCCAGGTATGACTACGGATTGTTAAAGAATCTGAGAGTCTACGGCCAAAGGAAGCCTCCTGCATTTGATCTCAGCCGTATTCCTAAGTCCTTGCCGCTGTGGATGGCGTACGGGGGCAGCGACGAGTTGTCCGATTGGACAGATCTGCAGCACACGATCAAGGAGCTGAAGTCGGTACCAGAATTGGTTTACCTTGAAAATTATGGCCATGTGGACTTCATTTTGAGCATGAAAGCGAAGGAAGATGTGTATGATCCTATGATCAAATTCTTGAAATCCTTGGGAAAGTCAGGTAGTTTGTAA

mRNA sequence

AATTCGCGTCTAATTTGTCTCGCTGTTACTTTGATGGTCAATCTTGTTCCTGAAGATGGCGAAGCCATTGATGGCTATGGTATTTCTTCTTTGCTTCTTCGCTCTCCATTCTGCCGATGATTCCTCTTATCGTTCCCTTGAGTACTCGATTCTCCCTCGCGCTCTGTCTGACCAGAAGTCCTTGTGCTCTCAACTTGTTCAACCTGTTGGCTATCCTTGCGCGGAGCATCAGATACAAACGAAGGATGGATTTTTATTGGGTCTTCAACGAGTATCTTCACGCGATGGAGAATTGGAAAAGCAAAAGGGCCCTCCAATTTTGCTACTCCATGGACTGTTCATGGGTGGCGATGCATGGTTTTTGAATTCTGCTAGAGAATCTTTAGGATTTATCCTTGCAGATAATGGATTTGATGTGTGGATAGGAAATGTGCGTGGAACTCGGTGGAGTCATGGGCATACATCCGTATCAGAGGATGAGAAGATAATTCTTGCCGCGGGATTTCATGAACTCAACTTTAAAAGCGATTGGGGAACAGTCCTTTTAGATGATCTTTGTGATCGGCTCGTTAACTGCATCAATGTTCTAAGTTCAATAACAGGTGAAAATTGTTGCCTTAATGGCTCACGGTTCGACTTATTTTTCGAATACGAACCACATCCATCATCAGCAAAAAACTTGCACCATCTTTTCCAAATGATCCGCAAGGGTACTTTTTCCAGGTATGACTACGGATTGTTAAAGAATCTGAGAGTCTACGGCCAAAGGAAGCCTCCTGCATTTGATCTCAGCCGTATTCCTAAGTCCTTGCCGCTGTGGATGGCGTACGGGGGCAGCGACGAGTTGTCCGATTGGACAGATCTGCAGCACACGATCAAGGAGCTGAAGTCGGTACCAGAATTGGTTTACCTTGAAAATTATGGCCATGTGGACTTCATTTTGAGCATGAAAGCGAAGGAAGATGTGTATGATCCTATGATCAAATTCTTGAAATCCTTGGGAAAGTCAGGTAGTTTGTAA

Coding sequence (CDS)

ATGGCGAAGCCATTGATGGCTATGGTATTTCTTCTTTGCTTCTTCGCTCTCCATTCTGCCGATGATTCCTCTTATCGTTCCCTTGAGTACTCGATTCTCCCTCGCGCTCTGTCTGACCAGAAGTCCTTGTGCTCTCAACTTGTTCAACCTGTTGGCTATCCTTGCGCGGAGCATCAGATACAAACGAAGGATGGATTTTTATTGGGTCTTCAACGAGTATCTTCACGCGATGGAGAATTGGAAAAGCAAAAGGGCCCTCCAATTTTGCTACTCCATGGACTGTTCATGGGTGGCGATGCATGGTTTTTGAATTCTGCTAGAGAATCTTTAGGATTTATCCTTGCAGATAATGGATTTGATGTGTGGATAGGAAATGTGCGTGGAACTCGGTGGAGTCATGGGCATACATCCGTATCAGAGGATGAGAAGATAATTCTTGCCGCGGGATTTCATGAACTCAACTTTAAAAGCGATTGGGGAACAGTCCTTTTAGATGATCTTTGTGATCGGCTCGTTAACTGCATCAATGTTCTAAGTTCAATAACAGGTGAAAATTGTTGCCTTAATGGCTCACGGTTCGACTTATTTTTCGAATACGAACCACATCCATCATCAGCAAAAAACTTGCACCATCTTTTCCAAATGATCCGCAAGGGTACTTTTTCCAGGTATGACTACGGATTGTTAAAGAATCTGAGAGTCTACGGCCAAAGGAAGCCTCCTGCATTTGATCTCAGCCGTATTCCTAAGTCCTTGCCGCTGTGGATGGCGTACGGGGGCAGCGACGAGTTGTCCGATTGGACAGATCTGCAGCACACGATCAAGGAGCTGAAGTCGGTACCAGAATTGGTTTACCTTGAAAATTATGGCCATGTGGACTTCATTTTGAGCATGAAAGCGAAGGAAGATGTGTATGATCCTATGATCAAATTCTTGAAATCCTTGGGAAAGTCAGGTAGTTTGTAA

Protein sequence

MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRALSDQKSLCSQLVQPVGYPCAEHQIQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFDVWIGNVRGTRWSHGHTSVSEDEKIILAAGFHELNFKSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIKELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKSGSL
Homology
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BLAST of Cp4.1LG15g04680 vs. ExPASy Swiss-Prot
Match: Q71DJ5 (Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 4.2e-93
Identity = 169/368 (45.92%), Postives = 216/368 (58.70%), Query Frame = 0 

Query: 38  SDQKSLCSQLVQPVGYPCAEHQIQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMG 97
           S   SLC+ L+ P  Y C EH IQTKDG++L LQRV+S    L  Q GPP+LL HGLFM 
Sbjct: 27  SPVNSLCADLIHPANYSCTEHSIQTKDGYILALQRVASLGPRL--QSGPPVLLQHGLFMA 86

Query: 98  GDAWFLNSARESLGFILADNGFDVWIGNVRGTRWSHGHTSVSEDEK-------------- 157
           GD WFLNS +ESLGFILAD+GFDVW+GNVRGTR+S+GH ++S+ +K              
Sbjct: 87  GDVWFLNSPKESLGFILADHGFDVWVGNVRGTRYSYGHVTLSDTDKEFWDWSWQDLAMYD 146

Query: 158 ------------------------------------------------------------ 217
                                                                       
Sbjct: 147 LAEMIQYLYSISNSKIFLVGHSQGTIMSFAALTQPHVAEMVEAAALLCPISYLDHVTAPL 206

Query: 218 -----------IILAAGFHELNFKSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSR 277
                      +++A G H++NF+SD    L+D LC+  ++C + L+SITG NCC N S+
Sbjct: 207 VERMVFMHLDQMVVALGLHQINFRSDMLVKLVDSLCEGHMDCTDFLTSITGTNCCFNASK 266

Query: 278 FDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSL 321
            + + +YEPHPSS KN+ HLFQMIRKGTF++YDYG  KNLR YG  KPP F LS IP SL
Sbjct: 267 IEYYLDYEPHPSSVKNIRHLFQMIRKGTFAQYDYGYFKNLRTYGLSKPPEFILSHIPASL 326

[Close]
BLAST of Cp4.1LG15g04680 vs. ExPASy Swiss-Prot
Match: Q67ZU1 (Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 7.2e-45
Identity = 113/365 (30.96%), Postives = 171/365 (46.85%), Query Frame = 0 

Query: 43  LCSQLVQPVGYPCAEHQIQTKDGFLLGLQRV-SSRDGEL--EKQKGPPILLLHGLFMGGD 102
           +C+  V   GY C EH + T+DG++L +QR+   R G +  +  K  P+L+ HG+ + G 
Sbjct: 51  ICASSVHIFGYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQPVLIQHGILVDGM 110

Query: 103 AWFLNSARESLGFILADNGFDVWIGNVRGTRWSHGH------------------------ 162
           +W LN A ++L  ILAD GFDVW+GN RGTR+S  H                        
Sbjct: 111 SWLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYDLP 170

Query: 163 ------------------------------------------------------TSVSED 222
                                                                 T+V  D
Sbjct: 171 AMFDHIHGLTGQKIHYLGHSLGTLIGFASFSEKGLVDQVRSAAMLSPVAYLSHMTTVIGD 230

Query: 223 --EKIILA-----AGFHELNFKSDWGTVLLDDLCDRL-VNCINVLSSITGENCCLNGSRF 282
              K  LA      G+ E N KS      +  +C +  ++C +++S ITG+NCCLN S  
Sbjct: 231 IAAKTFLAEATSILGWPEFNPKSGLVGDFIKAICLKAGIDCYDLVSVITGKNCCLNASTI 290

Query: 283 DLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLL-KNLRVYGQRKPPAFDLSRIPKSL 314
           DLF   EP  +S KN+ HL Q +R     +Y+YG   +N++ YGQ  PPA+++S IP  L
Sbjct: 291 DLFLANEPQSTSTKNMIHLAQTVRDKELRKYNYGSSDRNIKHYGQAIPPAYNISAIPHEL 350

[Close]
BLAST of Cp4.1LG15g04680 vs. ExPASy Swiss-Prot
Match: P04634 (Gastric triacylglycerol lipase OS=Rattus norvegicus OX=10116 GN=Lipf PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 9.4e-29
Identity = 93/360 (25.83%), Postives = 147/360 (40.83%), Query Frame = 0 

Query: 45  SQLVQPVGYPCAEHQIQTKDGFLLGLQRVSSRDGELEK-QKGPPILLLHGLFMGGDAWFL 104
           SQ++   GYPC E+++ T+DG++LG+ R+       E   K P + L HGL      W  
Sbjct: 35  SQMITYWGYPCQEYEVVTEDGYILGVYRIPHGKNNSENIGKRPVVYLQHGLIASATNWIA 94

Query: 105 NSARESLGFILADNGFDVWIGNVRGTRWSHGHTSVSEDEKIILAAGFHELN--------- 164
           N    SL F+LAD G+DVW+GN RG  WS  +   S D     A  F E+          
Sbjct: 95  NLPNNSLAFMLADAGYDVWLGNSRGNTWSRKNVYYSPDSVEFWAFSFDEMAKYDLPATIN 154

Query: 165 ------------------------------------------------------------ 224
                                                                       
Sbjct: 155 FIVQKTGQEKIHYVGHSQGTTIGFIAFSTNPTLAKKIKTFYALAPVATVKYTQSPLKKIS 214

Query: 225 ------FKSDWGTVLL-----------DDLCDRLV---NCINVLSSITG-ENCCLNGSRF 284
                 FK  +G  +             ++C R V    C N L    G +   LN SRF
Sbjct: 215 FIPTFLFKLMFGKKMFLPHTYFDDFLGTEVCSREVLDLLCSNTLFIFCGFDKKNLNVSRF 274

Query: 285 DLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGL-LKNLRVYGQRKPPAFDLSRIPKSL 313
           D++  + P  +S ++  H  Q++R G F  +++G   +N+  Y Q+ PP +D+S +   +
Sbjct: 275 DVYLGHNPAGTSVQDFLHWAQLVRSGKFQAFNWGSPSQNMLHYNQKTPPEYDVSAMTVPV 334

[Close]
BLAST of Cp4.1LG15g04680 vs. ExPASy Swiss-Prot
Match: Q29458 (Gastric triacylglycerol lipase OS=Bos taurus OX=9913 GN=LIPF PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 2.3e-27
Identity = 90/360 (25.00%), Postives = 146/360 (40.56%), Query Frame = 0 

Query: 45  SQLVQPVGYPCAEHQIQTKDGFLLGLQRV-SSRDGELEKQKGPPILLLHGLFMGGDAWFL 104
           SQ++   GYP   H++ T DG++L + R+   ++      + P + L HGL      W  
Sbjct: 35  SQMISYWGYPSEMHKVITADGYILQVYRIPHGKNNANHLGQRPVVFLQHGLLGSATNWIS 94

Query: 105 NSARESLGFILADNGFDVWIGNVRGTRWSHGHTSVSEDEKIILAAGFHELN--------- 164
           N  + SLGF+LAD G+DVW+GN RG  W+  H   S D     A  F E+          
Sbjct: 95  NLPKNSLGFLLADAGYDVWLGNSRGNTWAQEHLYYSPDSPEFWAFSFDEMAEYDLPSTID 154

Query: 165 ------------------------------------------------------------ 224
                                                                       
Sbjct: 155 FILRRTGQKKLHYVGHSQGTTIGFIAFSTSPTLAEKIKVFYALAPVATVKYTKSLFNKLA 214

Query: 225 ------FKSDWGTVLL-----------DDLCDRL---VNCINVLSSITG-ENCCLNGSRF 284
                 FK  +G  +             ++C R    V C N L +ITG +N   N SR 
Sbjct: 215 LIPHFLFKIIFGDKMFYPHTFLEQFLGVEMCSRETLDVLCKNALFAITGVDNKNFNMSRL 274

Query: 285 DLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGL-LKNLRVYGQRKPPAFDLSRIPKSL 313
           D++  + P  +S +N  H  Q ++ G F  +D+G   +NL  Y Q  PP ++L+ +   +
Sbjct: 275 DVYIAHNPAGTSVQNTLHWRQAVKSGKFQAFDWGAPYQNLMHYHQPTPPIYNLTAMNVPI 334

[Close]
BLAST of Cp4.1LG15g04680 vs. ExPASy Swiss-Prot
Match: P80035 (Gastric triacylglycerol lipase OS=Canis lupus familiaris OX=9615 GN=LIPF PE=1 SV=2)

HSP 1 Score: 120.9 bits (302), Expect = 2.6e-26
Identity = 92/363 (25.34%), Postives = 146/363 (40.22%), Query Frame = 0 

Query: 45  SQLVQPVGYPCAEHQIQTKDGFLLGLQRVS-SRDGELEKQKGPPILLLHGLFMGGDAWFL 104
           SQ++   GYP  E+++ T+DG++LG+ R+   R       + P   L HGL      W  
Sbjct: 36  SQMITYWGYPAEEYEVVTEDGYILGIDRIPYGRKNSENIGRRPVAFLQHGLLASATNWIS 95

Query: 105 NSARESLGFILADNGFDVWIGNVRGTRWSHGHTSVSEDEKIILAAGFHELN--------- 164
           N    SL FILAD G+DVW+GN RG  W+  +   S D     A  F E+          
Sbjct: 96  NLPNNSLAFILADAGYDVWLGNSRGNTWARRNLYYSPDSVEFWAFSFDEMAKYDLPATID 155

Query: 165 ------------------------------------------------------------ 224
                                                                       
Sbjct: 156 FILKKTGQDKLHYVGHSQGTTIGFIAFSTNPKLAKRIKTFYALAPVATVKYTETLLNKLM 215

Query: 225 ------FKSDWGT-----------VLLDDLCDRL---VNCINVLSSITG-ENCCLNGSRF 284
                 FK  +G             L  ++C R    + C N L  I G +   LN SR 
Sbjct: 216 LVPSFLFKLIFGNKIFYPHHFFDQFLATEVCSRETVDLLCSNALFIICGFDTMNLNMSRL 275

Query: 285 DLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGL-LKNLRVYGQRKPPAFDLSRIPKSL 313
           D++  + P  +S +N+ H  Q ++ G F  +D+G  ++N+  Y Q  PP ++L+ +   +
Sbjct: 276 DVYLSHNPAGTSVQNVLHWSQAVKSGKFQAFDWGSPVQNMMHYHQSMPPYYNLTDMHVPI 335

[Close]
BLAST of Cp4.1LG15g04680 vs. NCBI nr
Match: KAG7011633.1 (Triacylglycerol lipase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 638 bits (1646), Expect = 1.69e-230
Identity = 315/338 (93.20%), Postives = 317/338 (93.79%), Query Frame = 0 

Query: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRALSDQKSLCSQLVQPVGYPCAEHQI 60
           MAKPLMAMVFLLCFFALHSAD SSYRSLEYSILPR LSDQKSLCSQLVQP GYPCAEHQI
Sbjct: 1   MAKPLMAMVFLLCFFALHSADHSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
           QTKDGFLLGLQRVSS DG+LEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHTSVSEDEK-----------------IILAAGFHELNFKSDWGTVL 180
           VWIGNVRGTRWSHGHTS+SEDEK                 IILAAGFHELNFKSDWGTVL
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKHITAPLVRLMVDTHLDTIILAAGFHELNFKSDWGTVL 180

Query: 181 LDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSR 240
           LDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSR
Sbjct: 181 LDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSR 240

Query: 241 YDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIKELKSVPEL 300
           YDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIKELKSVPEL
Sbjct: 241 YDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIKELKSVPEL 300

Query: 301 VYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKSGSL 321
           VYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKSGSL
Sbjct: 301 VYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKSGSL 338

[Close]
BLAST of Cp4.1LG15g04680 vs. NCBI nr
Match: XP_023553815.1 (triacylglycerol lipase 1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 620 bits (1599), Expect = 4.72e-222
Identity = 319/404 (78.96%), Postives = 319/404 (78.96%), Query Frame = 0 

Query: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRALSDQKSLCSQLVQPVGYPCAEHQI 60
           MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRALSDQKSLCSQLVQPVGYPCAEHQI
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRALSDQKSLCSQLVQPVGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
           QTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHTSVSEDEK------------------------------------- 180
           VWIGNVRGTRWSHGHTSVSEDEK                                     
Sbjct: 121 VWIGNVRGTRWSHGHTSVSEDEKEFWDWSWEDLALYDLAGMIDYINSLTKRKIYVVGHSQ 180

Query: 181 ------------------------------------------------IILAAGFHELNF 240
                                                           IILAAGFHELNF
Sbjct: 181 GTIMSFAALTQPDIAKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 319
           IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

[Close]
BLAST of Cp4.1LG15g04680 vs. NCBI nr
Match: XP_023553816.1 (triacylglycerol lipase 1 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 618 bits (1593), Expect = 2.50e-221
Identity = 318/403 (78.91%), Postives = 318/403 (78.91%), Query Frame = 0 

Query: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRALSDQKSLCSQLVQPVGYPCAEHQI 60
           MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRALSDQKSLCSQLVQPVGYPCAEHQI
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRALSDQKSLCSQLVQPVGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
           QTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHTSVSEDEK------------------------------------- 180
           VWIGNVRGTRWSHGHTSVSEDEK                                     
Sbjct: 121 VWIGNVRGTRWSHGHTSVSEDEKEFWDWSWEDLALYDLAGMIDYINSLTKRKIYVVGHSQ 180

Query: 181 ------------------------------------------------IILAAGFHELNF 240
                                                           IILAAGFHELNF
Sbjct: 181 GTIMSFAALTQPDIAKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 318
           IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

[Close]
BLAST of Cp4.1LG15g04680 vs. NCBI nr
Match: XP_022952782.1 (triacylglycerol lipase 1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 613 bits (1582), Expect = 1.58e-219
Identity = 315/406 (77.59%), Postives = 318/406 (78.33%), Query Frame = 0 

Query: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRALSDQKSLCSQLVQPVGYPCAEHQI 60
           MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPR LSDQKSLCSQLVQP GYPCAEHQI
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
           QTKDGFLLGLQRVSS DG+LEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHTSVSEDEK------------------------------------- 180
           VWIGNVRGTRWSHGHTS+SEDEK                                     
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180

Query: 181 ------------------------------------------------IILAAGFHELNF 240
                                                           IILAAGFHELNF
Sbjct: 181 GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 321
           IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

[Close]
BLAST of Cp4.1LG15g04680 vs. NCBI nr
Match: XP_022952781.1 (triacylglycerol lipase 1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 612 bits (1577), Expect = 1.06e-218
Identity = 314/404 (77.72%), Postives = 316/404 (78.22%), Query Frame = 0 

Query: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRALSDQKSLCSQLVQPVGYPCAEHQI 60
           MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPR LSDQKSLCSQLVQP GYPCAEHQI
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
           QTKDGFLLGLQRVSS DG+LEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHTSVSEDEK------------------------------------- 180
           VWIGNVRGTRWSHGHTS+SEDEK                                     
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180

Query: 181 ------------------------------------------------IILAAGFHELNF 240
                                                           IILAAGFHELNF
Sbjct: 181 GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 319
           IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

[Close]
BLAST of Cp4.1LG15g04680 vs. ExPASy TrEMBL
Match: A0A6J1GLC0 (Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1)

HSP 1 Score: 613 bits (1582), Expect = 7.67e-220
Identity = 315/406 (77.59%), Postives = 318/406 (78.33%), Query Frame = 0 

Query: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRALSDQKSLCSQLVQPVGYPCAEHQI 60
           MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPR LSDQKSLCSQLVQP GYPCAEHQI
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
           QTKDGFLLGLQRVSS DG+LEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHTSVSEDEK------------------------------------- 180
           VWIGNVRGTRWSHGHTS+SEDEK                                     
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180

Query: 181 ------------------------------------------------IILAAGFHELNF 240
                                                           IILAAGFHELNF
Sbjct: 181 GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 321
           IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

[Close]
BLAST of Cp4.1LG15g04680 vs. ExPASy TrEMBL
Match: A0A6J1GL56 (Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1)

HSP 1 Score: 612 bits (1577), Expect = 5.13e-219
Identity = 314/404 (77.72%), Postives = 316/404 (78.22%), Query Frame = 0 

Query: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRALSDQKSLCSQLVQPVGYPCAEHQI 60
           MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPR LSDQKSLCSQLVQP GYPCAEHQI
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
           QTKDGFLLGLQRVSS DG+LEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHTSVSEDEK------------------------------------- 180
           VWIGNVRGTRWSHGHTS+SEDEK                                     
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180

Query: 181 ------------------------------------------------IILAAGFHELNF 240
                                                           IILAAGFHELNF
Sbjct: 181 GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 319
           IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

[Close]
BLAST of Cp4.1LG15g04680 vs. ExPASy TrEMBL
Match: A0A6J1GMQ3 (Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1)

HSP 1 Score: 609 bits (1571), Expect = 2.71e-218
Identity = 313/403 (77.67%), Postives = 315/403 (78.16%), Query Frame = 0 

Query: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRALSDQKSLCSQLVQPVGYPCAEHQI 60
           MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPR LSDQKSLCSQLVQP GYPCAEHQI
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
           QTKDGFLLGLQRVSS DG+LEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHTSVSEDEK------------------------------------- 180
           VWIGNVRGTRWSHGHTS+SEDEK                                     
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180

Query: 181 ------------------------------------------------IILAAGFHELNF 240
                                                           IILAAGFHELNF
Sbjct: 181 GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 318
           IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

[Close]
BLAST of Cp4.1LG15g04680 vs. ExPASy TrEMBL
Match: A0A6J1I8F7 (triacylglycerol lipase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470951 PE=4 SV=1)

HSP 1 Score: 602 bits (1553), Expect = 1.22e-214
Identity = 309/404 (76.49%), Postives = 312/404 (77.23%), Query Frame = 0 

Query: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRALSDQKSLCSQLVQPVGYPCAEHQI 60
           MAKPLMAMVFLLCFFALHSA+DSSYRSLEYSILPRALSDQKSLCSQLVQP GYPCAEHQI
Sbjct: 1   MAKPLMAMVFLLCFFALHSANDSSYRSLEYSILPRALSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
           QTKDGFLLGLQRVSS DG+LEKQKGPPILLLHGLFMGGDAWFLNSA ESLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSATESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHTSVSEDEK------------------------------------- 180
           VWIGNVRGTRWSHGHTS+SEDEK                                     
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINPLTKRKIYVVGHSQ 180

Query: 181 ------------------------------------------------IILAAGFHELNF 240
                                                           IILAAGFHELNF
Sbjct: 181 GTIMSFAALTQPDIAKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGT LLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTALLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 319
           IRKGTFSRYDYGL KNLRVYGQRKPPAFDLS IPKSLPLWMAYGGSDELSDWTDLQHTIK
Sbjct: 301 IRKGTFSRYDYGLFKNLRVYGQRKPPAFDLSHIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

[Close]
BLAST of Cp4.1LG15g04680 vs. ExPASy TrEMBL
Match: A0A6J1I5U4 (Lipase OS=Cucurbita maxima OX=3661 GN=LOC111470951 PE=3 SV=1)

HSP 1 Score: 600 bits (1547), Expect = 1.23e-214
Identity = 308/403 (76.43%), Postives = 311/403 (77.17%), Query Frame = 0 

Query: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRALSDQKSLCSQLVQPVGYPCAEHQI 60
           MAKPLMAMVFLLCFFALHSA+DSSYRSLEYSILPRALSDQKSLCSQLVQP GYPCAEHQI
Sbjct: 1   MAKPLMAMVFLLCFFALHSANDSSYRSLEYSILPRALSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
           QTKDGFLLGLQRVSS DG+LEKQKGPPILLLHGLFMGGDAWFLNSA ESLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSATESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHTSVSEDEK------------------------------------- 180
           VWIGNVRGTRWSHGHTS+SEDEK                                     
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINPLTKRKIYVVGHSQ 180

Query: 181 ------------------------------------------------IILAAGFHELNF 240
                                                           IILAAGFHELNF
Sbjct: 181 GTIMSFAALTQPDIAKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGT LLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTALLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 318
           IRKGTFSRYDYGL KNLRVYGQRKPPAFDLS IPKSLPLWMAYGGSDELSDWTDLQHTIK
Sbjct: 301 IRKGTFSRYDYGLFKNLRVYGQRKPPAFDLSHIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

[Close]
BLAST of Cp4.1LG15g04680 vs. TAIR 10
Match: AT2G15230.1 (lipase 1 )

HSP 1 Score: 342.8 bits (878), Expect = 3.0e-94
Identity = 169/368 (45.92%), Postives = 216/368 (58.70%), Query Frame = 0 

Query: 38  SDQKSLCSQLVQPVGYPCAEHQIQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMG 97
           S   SLC+ L+ P  Y C EH IQTKDG++L LQRV+S    L  Q GPP+LL HGLFM 
Sbjct: 27  SPVNSLCADLIHPANYSCTEHSIQTKDGYILALQRVASLGPRL--QSGPPVLLQHGLFMA 86

Query: 98  GDAWFLNSARESLGFILADNGFDVWIGNVRGTRWSHGHTSVSEDEK-------------- 157
           GD WFLNS +ESLGFILAD+GFDVW+GNVRGTR+S+GH ++S+ +K              
Sbjct: 87  GDVWFLNSPKESLGFILADHGFDVWVGNVRGTRYSYGHVTLSDTDKEFWDWSWQDLAMYD 146

Query: 158 ------------------------------------------------------------ 217
                                                                       
Sbjct: 147 LAEMIQYLYSISNSKIFLVGHSQGTIMSFAALTQPHVAEMVEAAALLCPISYLDHVTAPL 206

Query: 218 -----------IILAAGFHELNFKSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSR 277
                      +++A G H++NF+SD    L+D LC+  ++C + L+SITG NCC N S+
Sbjct: 207 VERMVFMHLDQMVVALGLHQINFRSDMLVKLVDSLCEGHMDCTDFLTSITGTNCCFNASK 266

Query: 278 FDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSL 321
            + + +YEPHPSS KN+ HLFQMIRKGTF++YDYG  KNLR YG  KPP F LS IP SL
Sbjct: 267 IEYYLDYEPHPSSVKNIRHLFQMIRKGTFAQYDYGYFKNLRTYGLSKPPEFILSHIPASL 326

[Close]
BLAST of Cp4.1LG15g04680 vs. TAIR 10
Match: AT5G14180.1 (Myzus persicae-induced lipase 1 )

HSP 1 Score: 182.6 bits (462), Expect = 5.1e-46
Identity = 113/365 (30.96%), Postives = 171/365 (46.85%), Query Frame = 0 

Query: 43  LCSQLVQPVGYPCAEHQIQTKDGFLLGLQRV-SSRDGEL--EKQKGPPILLLHGLFMGGD 102
           +C+  V   GY C EH + T+DG++L +QR+   R G +  +  K  P+L+ HG+ + G 
Sbjct: 51  ICASSVHIFGYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQPVLIQHGILVDGM 110

Query: 103 AWFLNSARESLGFILADNGFDVWIGNVRGTRWSHGH------------------------ 162
           +W LN A ++L  ILAD GFDVW+GN RGTR+S  H                        
Sbjct: 111 SWLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYDLP 170

Query: 163 ------------------------------------------------------TSVSED 222
                                                                 T+V  D
Sbjct: 171 AMFDHIHGLTGQKIHYLGHSLGTLIGFASFSEKGLVDQVRSAAMLSPVAYLSHMTTVIGD 230

Query: 223 --EKIILA-----AGFHELNFKSDWGTVLLDDLCDRL-VNCINVLSSITGENCCLNGSRF 282
              K  LA      G+ E N KS      +  +C +  ++C +++S ITG+NCCLN S  
Sbjct: 231 IAAKTFLAEATSILGWPEFNPKSGLVGDFIKAICLKAGIDCYDLVSVITGKNCCLNASTI 290

Query: 283 DLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLL-KNLRVYGQRKPPAFDLSRIPKSL 314
           DLF   EP  +S KN+ HL Q +R     +Y+YG   +N++ YGQ  PPA+++S IP  L
Sbjct: 291 DLFLANEPQSTSTKNMIHLAQTVRDKELRKYNYGSSDRNIKHYGQAIPPAYNISAIPHEL 350

[Close]
BLAST of Cp4.1LG15g04680 vs. TAIR 10
Match: AT1G73920.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 58.5 bits (140), Expect = 1.1e-08
Identity = 31/111 (27.93%), Postives = 51/111 (45.95%), Query Frame = 0 

Query: 33  LPRALSDQKSLCSQLVQPVGYPCAEHQIQTKDGFLLGLQRVSSRDGELEKQKGPPILLLH 92
           L  +++     C  ++  +GYP    ++ T DG++L L+R+  RD          + L H
Sbjct: 285 LYNSMNTDTRTCQDVITELGYPYEAIRVITSDGYVLVLERIPRRDAR------KAVFLQH 344

Query: 93  GLFMGGDAWFLNSARESLGFILADNGFDVWIGNVRG-TRWSHGHTSVSEDE 143
           G+      W  N    S  F   D G+DV++GN RG     H + ++S  E
Sbjct: 345 GVLDSSMGWVSNGVVGSPAFAAYDQGYDVFLGNFRGLVSRDHVNKNISSKE 389

[Close]
BLAST of Cp4.1LG15g04680 vs. TAIR 10
Match: AT1G73920.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 58.5 bits (140), Expect = 1.1e-08
Identity = 31/111 (27.93%), Postives = 51/111 (45.95%), Query Frame = 0 

Query: 33  LPRALSDQKSLCSQLVQPVGYPCAEHQIQTKDGFLLGLQRVSSRDGELEKQKGPPILLLH 92
           L  +++     C  ++  +GYP    ++ T DG++L L+R+  RD          + L H
Sbjct: 216 LYNSMNTDTRTCQDVITELGYPYEAIRVITSDGYVLVLERIPRRDAR------KAVFLQH 275

Query: 93  GLFMGGDAWFLNSARESLGFILADNGFDVWIGNVRG-TRWSHGHTSVSEDE 143
           G+      W  N    S  F   D G+DV++GN RG     H + ++S  E
Sbjct: 276 GVLDSSMGWVSNGVVGSPAFAAYDQGYDVFLGNFRGLVSRDHVNKNISSKE 320

[Close]
BLAST of Cp4.1LG15g04680 vs. TAIR 10
Match: AT1G18460.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 54.7 bits (130), Expect = 1.6e-07
Identity = 27/85 (31.76%), Postives = 40/85 (47.06%), Query Frame = 0 

Query: 44  CSQLVQPVGYPCAEHQIQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFL 103
           C  ++  +GYP    ++ T DG+ L L+R+  RD          + L HG+      W  
Sbjct: 294 CQDVITELGYPYEAIRVVTSDGYGLLLERIPRRDAR------KAVYLQHGVMDSSMGWVS 353

Query: 104 NSARESLGFILADNGFDVWIGNVRG 129
           N    S  F   D G+DV++GN RG
Sbjct: 354 NGVVGSPAFAAYDQGYDVFLGNFRG 372

The following BLAST results are available for this feature:
BLAST of Cp4.1LG15g04680 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q71DJ54.2e-9345.92Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1[more]
Q67ZU17.2e-4530.96Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1[more]
P046349.4e-2925.83Gastric triacylglycerol lipase OS=Rattus norvegicus OX=10116 GN=Lipf PE=1 SV=1[more]
Q294582.3e-2725.00Gastric triacylglycerol lipase OS=Bos taurus OX=9913 GN=LIPF PE=1 SV=1[more]
P800352.6e-2625.34Gastric triacylglycerol lipase OS=Canis lupus familiaris OX=9615 GN=LIPF PE=1 SV... [more]
BLAST of Cp4.1LG15g04680 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7011633.11.69e-23093.20Triacylglycerol lipase 1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023553815.14.72e-22278.96triacylglycerol lipase 1 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023553816.12.50e-22178.91triacylglycerol lipase 1 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022952782.11.58e-21977.59triacylglycerol lipase 1 isoform X2 [Cucurbita moschata][more]
XP_022952781.11.06e-21877.72triacylglycerol lipase 1 isoform X1 [Cucurbita moschata][more]
BLAST of Cp4.1LG15g04680 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1GLC07.67e-22077.59Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1[more]
A0A6J1GL565.13e-21977.72Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1[more]
A0A6J1GMQ32.71e-21877.67Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1[more]
A0A6J1I8F71.22e-21476.49triacylglycerol lipase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470951 ... [more]
A0A6J1I5U41.23e-21476.43Lipase OS=Cucurbita maxima OX=3661 GN=LOC111470951 PE=3 SV=1[more]
BLAST of Cp4.1LG15g04680 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G15230.13.0e-9445.92lipase 1 [more]
AT5G14180.15.1e-4630.96Myzus persicae-induced lipase 1 [more]
AT1G73920.11.1e-0827.93alpha/beta-Hydrolases superfamily protein [more]
AT1G73920.21.1e-0827.93alpha/beta-Hydrolases superfamily protein [more]
AT1G18460.11.6e-0731.76alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 145..314
e-value: 8.4E-40
score: 138.7
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 70..144
e-value: 8.2E-7
score: 30.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 44..313
IPR006693Partial AB-hydrolase lipase domainPFAMPF04083Abhydro_lipasecoord: 45..104
e-value: 7.9E-18
score: 63.8
NoneNo IPR availablePANTHERPTHR11005LYSOSOMAL ACID LIPASE-RELATEDcoord: 26..142
NoneNo IPR availablePANTHERPTHR11005LYSOSOMAL ACID LIPASE-RELATEDcoord: 146..315
NoneNo IPR availablePANTHERPTHR11005:SF118TRIACYLGLYCEROL LIPASE 1coord: 26..142
NoneNo IPR availablePANTHERPTHR11005:SF118TRIACYLGLYCEROL LIPASE 1coord: 146..315

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG15g04680.1Cp4.1LG15g04680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
biological_process GO:0048580 regulation of post-embryonic development
molecular_function GO:0016788 hydrolase activity, acting on ester bonds