Cp4.1LG15g04300 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG15g04300
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionTubulin alpha chain
LocationCp4.1LG15: 5435065 .. 5437820 (-)
RNA-Seq ExpressionCp4.1LG15g04300
SyntenyCp4.1LG15g04300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AATAAAAAGAAAAAACGGTTACAACTTCAAAATCACAAACTTAAAAAGTTAACCATGGTTAATTTCCAGCCAATTAAAATTAATTACCTTAACTAACCATGGTTAACCTCCGCTTATCCAAATTTCTCCCTCCTATTTTTCCAACTATAAAACGGATCTTCGTTTTGTTTTCTTCATTTTCCATTGAAACATACATTAACCGAAGAAGGCGTCTTCATAAACGCCTTCGTCTCCATTTTCGACCTTCGTTTGATCCGATTTTCTGTTCTTCGTCGATTATTGCTTTAGCTTGCTTTGTTCTTTTGGCTGGAGGAAAATGAGGGAGTGCATTTCGATTCACATCGGTCAGGCTGGGATTCAGGTCGGCAATGCGTGCTGGGAACTCTACTGCCTTGAACATGGAATTCAGGTATATCTCCTTGTAACTTCTTCGTTTTTTTTTTTGTTTCACTTGCGGTTATGATGTATTTGTATTCGCTTTCTTTTTAATCTGGTTTCTTTTTGCTTTCTGTATCTTTTCTTACTGCATGTCTGCTTGATCGATTTTGAATGAATTCGTAACGGTACTTCTGTATTTCGTAATTTTTGTTTGGTAAAACTTTGATCCTGCGCTAATTTGCTGGTTATTCTGATGTATGTATCTGTTTTGAGTTCCTGTATGGATCTTTAAGTTTGGACATCGGATAGGATCTTGTTAATTTTTTTTTTTTTTCTATAGAAGTAGATAGTTTTCCCTCCTAATGTTTCTGTTTTCTCTTTCAATTTTTCCGTGATATGATCTACATTTGTGAAAATAGCTCATTGTTTCTCATTACATTTTCTACGTTTAGAAGTTTTACGTTGTTATAAATGAACAGAAATGAATATATAGTTTCAGAATTCTATGCGAGGGCGAACGCTTTCTTTTCTTTTTCCATAAGCATCGTAATGAATTTAAAATTTGAATTTCAAGTAGGCTAAATTTTGTGGCCTGTTTTTCAGCCTGATGGCCAAATGCCGGGTGACACGACTGTAGGCGGAGGCGATGATGCCTTCAACACCTTCTTCAGTGAAACAGGTGCAGGCAAGCATGTTCCTCGAGCTCTTTTCGTTGATCTCGAGCCTACTGTCATCGATGAGGTGAGGACTGGAATGTATCGCCAGTTGTTCCATCCTGAACAGCTAATCAGCGGTAAGGAAGATGCTGCCAACAACTTTGCTCGCGGCCACTACACCAGTAAGCATCTCAATTGGCACATCCGTATAAAGTTACTCAATCAATATTGAACATACACCACAGTTTTTTCATGACTGTGTTATGGTAATTGGAAAAGTTCTGTTTCAGTTGGCAAGGAGATCGCTGATCTCTGTTTGGATCGCATTAGAAAGCTCGCTGACAATTGCACTGGACTCCAAGGCTTCCTTGTTTTTAACGCTGTCGGTGGGGGTACTGGGTCTGGTCTTGGCTCTCTCCTGTTGGAGCGTTTGTCTGTTGACTATGGCAAGAAATCAAAGCTTGGTTTCACAGTTTATCCCTCTCCACAAGTTTCCACCTCTGTTGTTGAACCCTACAACAGTGTTCTCTCAACCCACTCTCTCCTTGAACATACAGATGTTTCTGTGCTCCTTGACAACGAAGCCATTTATGACATTTGTAGACGTTCCCTTGCAATTGAGCGACCCACCTACACAAACCTCAACCGCCTCATCTCTCAGGTACATCTATCTTAACTGTGTTTGATTTAGGAAATGGGCAGGTAAGAAATGTGGGAAGTCCCACTTTCTTAATTTAGGAATTAAGAAATCATCATGGATTAATAAACTATGGCATGAGGCCTTTTGAGAAAGTCTAAAGTAAACTCCTTATGTTTAAAGTGGACAATATCATATTCTTGTGGAGAGTCAGGCATTTAGGGAATAATCATTGATTTATAATAGGGAATAATCATTGATTTATAATCATAGATTATTATCTCTAATGGTACGAGGAATTTTAGGAAAGCATAAAACAAAGCCACGAGATGTTATGCTTTGTATTATGTATTATATCATATCATGCAAATTCGTAGTTCCTAACATTATTTTTTCGTAAAGATTTACTGTTCCTTTGGTGTGATGTTCAGGTGATTTCATCCCTGACAGCATCTCTACGGTTCGATGGAGCTTTGAATGTGGATGTAACCGAGTTCCAAACGAATTTGGTCCCATACCCAAGAATCCACTTCATGCTTTCCTCCTATGCTCCAGTGATCTCCGCAGAGAAGGCATACCACGAACAACTCTCTGTAGCGGAGATCACCAGCAGCGCCTTCGAGCCATCTTCCATGATGGCCAAGTGCGATCCCCGCAATGGGAAGTACATGGCTTGCTGTCTGATGTACAGAGGAGATGTTGTGCCAAAGGACGCCAACGCCGCCGTTGCCACCATAAAAACGAAGCGCACGATCCAGTTCGTGGACTGGTGCCCCACTGGTTTCAAGTGCGGGATCAACTACCAGGCGCCGACGGTGGTTCCGGGTGGCGACCTGGCCAGAGTGCAGAGGGCAATCTGTATGATATCGAACTCCACCAGCGTGGCAGAGGTGTTCTGTCGCATAGACCACAAGTTTGATCTGATGTATGCGAAGAGGGCGTTCGTGCATTGGTACGTGGGGGAGGGCATGGAGGAGGGAGAGTTCTCTGAAGCTCGTGAGGATCTTGCGGCCCTTGAGAAGGATTATGAGGAAGTGGGTGCTGAGTCTGATGAAGGTGGTGATGATGACGATCAGGAGGAGTAC

mRNA sequence

AATAAAAAGAAAAAACGGTTACAACTTCAAAATCACAAACTTAAAAAGTTAACCATGGTTAATTTCCAGCCAATTAAAATTAATTACCTTAACTAACCATGGTTAACCTCCGCTTATCCAAATTTCTCCCTCCTATTTTTCCAACTATAAAACGGATCTTCGTTTTGTTTTCTTCATTTTCCATTGAAACATACATTAACCGAAGAAGGCGTCTTCATAAACGCCTTCGTCTCCATTTTCGACCTTCGTTTGATCCGATTTTCTGTTCTTCGTCGATTATTGCTTTAGCTTGCTTTGTTCTTTTGGCTGGAGGAAAATGAGGGAGTGCATTTCGATTCACATCGGTCAGGCTGGGATTCAGGTCGGCAATGCGTGCTGGGAACTCTACTGCCTTGAACATGGAATTCAGCCTGATGGCCAAATGCCGGGTGACACGACTGTAGGCGGAGGCGATGATGCCTTCAACACCTTCTTCAGTGAAACAGGTGCAGGCAAGCATGTTCCTCGAGCTCTTTTCGTTGATCTCGAGCCTACTGTCATCGATGAGGTGAGGACTGGAATGTATCGCCAGTTGTTCCATCCTGAACAGCTAATCAGCGGTAAGGAAGATGCTGCCAACAACTTTGCTCGCGGCCACTACACCATTGGCAAGGAGATCGCTGATCTCTGTTTGGATCGCATTAGAAAGCTCGCTGACAATTGCACTGGACTCCAAGGCTTCCTTGTTTTTAACGCTGTCGGTGGGGGTACTGGGTCTGGTCTTGGCTCTCTCCTGTTGGAGCGTTTGTCTGTTGACTATGGCAAGAAATCAAAGCTTGGTTTCACAGTTTATCCCTCTCCACAAGTTTCCACCTCTGTTGTTGAACCCTACAACAGTGTTCTCTCAACCCACTCTCTCCTTGAACATACAGATGTTTCTGTGCTCCTTGACAACGAAGCCATTTATGACATTTGTAGACGTTCCCTTGCAATTGAGCGACCCACCTACACAAACCTCAACCGCCTCATCTCTCAGTTCCAAACGAATTTGGTCCCATACCCAAGAATCCACTTCATGCTTTCCTCCTATGCTCCAGTGATCTCCGCAGAGAAGGCATACCACGAACAACTCTCTGTAGCGGAGATCACCAGCAGCGCCTTCGAGCCATCTTCCATGATGGCCAAGTGCGATCCCCGCAATGGGAAGTACATGGCTTGCTGTCTGATGTACAGAGGAGATGTTGTGCCAAAGGACGCCAACGCCGCCGTTGCCACCATAAAAACGAAGCGCACGATCCAGTTCGTGGACTGGTGCCCCACTGGTTTCAAGTGCGGGATCAACTACCAGGCGCCGACGGTGGTTCCGGGTGGCGACCTGGCCAGAGTGCAGAGGGCAATCTGTATGATATCGAACTCCACCAGCGTGGCAGAGGTGTTCTGTCGCATAGACCACAAGTTTGATCTGATGTATGCGAAGAGGGCGTTCGTGCATTGGTACGTGGGGGAGGGCATGGAGGAGGGAGAGTTCTCTGAAGCTCGTGAGGATCTTGCGGCCCTTGAGAAGGATTATGAGGAAGTGGGTGCTGAGTCTGATGAAGGTGGTGATGATGACGATCAGGAGGAGTAC

Coding sequence (CDS)

ATGAGGGAGTGCATTTCGATTCACATCGGTCAGGCTGGGATTCAGGTCGGCAATGCGTGCTGGGAACTCTACTGCCTTGAACATGGAATTCAGCCTGATGGCCAAATGCCGGGTGACACGACTGTAGGCGGAGGCGATGATGCCTTCAACACCTTCTTCAGTGAAACAGGTGCAGGCAAGCATGTTCCTCGAGCTCTTTTCGTTGATCTCGAGCCTACTGTCATCGATGAGGTGAGGACTGGAATGTATCGCCAGTTGTTCCATCCTGAACAGCTAATCAGCGGTAAGGAAGATGCTGCCAACAACTTTGCTCGCGGCCACTACACCATTGGCAAGGAGATCGCTGATCTCTGTTTGGATCGCATTAGAAAGCTCGCTGACAATTGCACTGGACTCCAAGGCTTCCTTGTTTTTAACGCTGTCGGTGGGGGTACTGGGTCTGGTCTTGGCTCTCTCCTGTTGGAGCGTTTGTCTGTTGACTATGGCAAGAAATCAAAGCTTGGTTTCACAGTTTATCCCTCTCCACAAGTTTCCACCTCTGTTGTTGAACCCTACAACAGTGTTCTCTCAACCCACTCTCTCCTTGAACATACAGATGTTTCTGTGCTCCTTGACAACGAAGCCATTTATGACATTTGTAGACGTTCCCTTGCAATTGAGCGACCCACCTACACAAACCTCAACCGCCTCATCTCTCAGTTCCAAACGAATTTGGTCCCATACCCAAGAATCCACTTCATGCTTTCCTCCTATGCTCCAGTGATCTCCGCAGAGAAGGCATACCACGAACAACTCTCTGTAGCGGAGATCACCAGCAGCGCCTTCGAGCCATCTTCCATGATGGCCAAGTGCGATCCCCGCAATGGGAAGTACATGGCTTGCTGTCTGATGTACAGAGGAGATGTTGTGCCAAAGGACGCCAACGCCGCCGTTGCCACCATAAAAACGAAGCGCACGATCCAGTTCGTGGACTGGTGCCCCACTGGTTTCAAGTGCGGGATCAACTACCAGGCGCCGACGGTGGTTCCGGGTGGCGACCTGGCCAGAGTGCAGAGGGCAATCTGTATGATATCGAACTCCACCAGCGTGGCAGAGGTGTTCTGTCGCATAGACCACAAGTTTGATCTGATGTATGCGAAGAGGGCGTTCGTGCATTGGTACGTGGGGGAGGGCATGGAGGAGGGAGAGTTCTCTGAAGCTCGTGAGGATCTTGCGGCCCTTGAGAAGGATTATGAGGAAGTGGGTGCTGAGTCTGATGAAGGTGGTGATGATGACGATCAGGAGGAGTAC

Protein sequence

MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGKHVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLDRIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSSMMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAPTVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAESDEGGDDDDQEEY
Homology
BLAST of Cp4.1LG15g04300 vs. ExPASy Swiss-Prot
Match: Q9ZRR5 (Tubulin alpha-3 chain OS=Hordeum vulgare OX=4513 GN=TUBA3 PE=2 SV=1)

HSP 1 Score: 822.4 bits (2123), Expect = 2.4e-237
Identity = 408/451 (90.47%), Postives = 418/451 (92.68%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGD TVGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDKTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120
           HVPRA+FVDLEPTVIDEVRTG YRQLFHPEQLISGKEDAANNFARGHYTIGKEI DLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ------- 240
           VVEPYNSVLSTHSLLEHTDV+VLLDNEAIYDICRRSL IERPTYTNLNRL+SQ       
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPTYTNLNRLVSQVISSLTA 240

Query: 241 --------------FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300
                         FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEIT+SAFEPSS
Sbjct: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360
           MMAKCDPR+GKYMACCLMYRGDVVPKD NAAVATIKTKRTIQFVDWCPTGFKCGINYQ P
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           +VVPGGDLA+VQRA+CMISNSTSV EVF RIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 SVVPGGDLAKVQRAVCMISNSTSVVEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESDEGGDDDDQEEY 431
           AREDLAALEKDYEEVGAE DEG D D+ +EY
Sbjct: 421 AREDLAALEKDYEEVGAEFDEGEDGDEGDEY 451

BLAST of Cp4.1LG15g04300 vs. ExPASy Swiss-Prot
Match: P33629 (Tubulin alpha chain OS=Prunus dulcis OX=3755 GN=TUBA PE=2 SV=1)

HSP 1 Score: 821.6 bits (2121), Expect = 4.1e-237
Identity = 408/449 (90.87%), Postives = 417/449 (92.87%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGD TVGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDKTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120
           HVPRA+FVDLEPTVIDEVRTG YRQLFHPEQLISGKEDAANNFARGHYTIGKEI DLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ------- 240
           VVEPYNSVLSTHSLLEHTDV+VLLDNEAIYDICRRSL IERPTYTNLNRL+SQ       
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPTYTNLNRLVSQVISSLTA 240

Query: 241 --------------FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300
                         FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEIT+SAFEPSS
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360
           MMAKCDPR+GKYMACCLMYRGDVVPKD NAAVATIKTKRTIQFVDWCPTGFKCGINYQ P
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLA+VQRA+CMISNSTSVAEVF RIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESDEGGDDDDQE 429
           AREDLAALEKDYEEVGAES EG DD+  +
Sbjct: 421 AREDLAALEKDYEEVGAESAEGEDDEGDD 449

BLAST of Cp4.1LG15g04300 vs. ExPASy Swiss-Prot
Match: P46259 (Tubulin alpha-1 chain OS=Pisum sativum OX=3888 GN=TUBA1 PE=3 SV=1)

HSP 1 Score: 821.2 bits (2120), Expect = 5.3e-237
Identity = 410/452 (90.71%), Postives = 419/452 (92.70%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGD TVGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDKTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120
           HVPRA+FVDLEPTVIDEVRTG YRQLFHPEQLISGKEDAANNFARGHYTIGKEI DLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ------- 240
           VVEPYNSVLSTHSLLEHTDV+VLLDNEAIYDICRRSL IERPTYTNLNRL+SQ       
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPTYTNLNRLVSQVISSLTA 240

Query: 241 --------------FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300
                         FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEIT+SAFEPSS
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360
           MMAKCDPR+GKYMACCLMYRGDVVPKD NAAVATIKTKRTIQFVDWCPTGFKCGINYQ P
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLA+VQRA+CMISNSTSVAEVF RIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESDEGGDDD-DQEEY 431
           AREDLAALEKDYEEVGAES +G DD   +EEY
Sbjct: 421 AREDLAALEKDYEEVGAESGDGDDDGLGEEEY 452

BLAST of Cp4.1LG15g04300 vs. ExPASy Swiss-Prot
Match: Q6VAF9 (Tubulin alpha-4 chain OS=Gossypium hirsutum OX=3635 PE=2 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 9.1e-237
Identity = 409/451 (90.69%), Postives = 418/451 (92.68%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMP D TVGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDKTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120
           HVPRA+FVDLEPTVIDEVRTG YRQLFHPEQLISGKEDAANNFARGHYTIGKEI DLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ------- 240
           VVEPYNSVLSTHSLLEHTDV+VLLDNEAIYDICRRSL IERPTYTNLNRL+SQ       
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPTYTNLNRLVSQVISSLTA 240

Query: 241 --------------FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300
                         FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEIT+SAFEPSS
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360
           MMAKCDPR+GKYMACCLMYRGDVVPKD NAAVATIKTKRTIQFVDWCPTGFKCGINYQ P
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLA+VQRA+CMISNSTSVAEVF RIDHKFDLMY KRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYTKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESDEGGDDDDQEEY 431
           AREDLAALEKDYEEVGAES E GD+ D+EEY
Sbjct: 421 AREDLAALEKDYEEVGAESGE-GDEGDEEEY 450

BLAST of Cp4.1LG15g04300 vs. ExPASy Swiss-Prot
Match: Q96460 (Tubulin alpha-2 chain OS=Hordeum vulgare OX=4513 GN=TUBA2 PE=2 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 1.6e-236
Identity = 406/451 (90.02%), Postives = 417/451 (92.46%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGD TVGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDKTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120
           HVPRA+FVDLEPTVIDEVRTG YRQLFHPEQLISGKEDAANNFARGHYTIGKEI DLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKL+DNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLSDNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ------- 240
           VVEPYNSVLSTHSLLEHTDVS+LLDNEAIYDICRRSL IERPTYTNLNRL+SQ       
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVSILLDNEAIYDICRRSLDIERPTYTNLNRLVSQVISSLTA 240

Query: 241 --------------FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300
                         FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEIT+SAFEPSS
Sbjct: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360
           MMAKCDPR+GKYMACCLMYRGDVVPKD NAAVATIKTKRTIQFVDWCPTGFKCGINYQ P
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
            VVPGGDLA+VQRA+CMISNSTSV EVF RIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 GVVPGGDLAKVQRAVCMISNSTSVVEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESDEGGDDDDQEEY 431
           AREDLAALEKDYEEVGAE D+G D D+ +EY
Sbjct: 421 AREDLAALEKDYEEVGAEFDDGEDGDEGDEY 451

BLAST of Cp4.1LG15g04300 vs. NCBI nr
Match: XP_022952112.1 (tubulin alpha chain-like [Cucurbita moschata] >XP_023553814.1 tubulin alpha chain-like [Cucurbita pepo subsp. pepo] >KAG6571909.1 Tubulin alpha chain, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 855 bits (2210), Expect = 1.06e-312
Identity = 430/451 (95.34%), Postives = 430/451 (95.34%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120
           HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD
Sbjct: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ------- 240
           VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ       
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQVISSLTA 240

Query: 241 --------------FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300
                         FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300

Query: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360
           MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP
Sbjct: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360

Query: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESDEGGDDDDQEEY 430
           AREDLAALEKDYEEVGAESDEGGDDDDQEEY
Sbjct: 421 AREDLAALEKDYEEVGAESDEGGDDDDQEEY 451

BLAST of Cp4.1LG15g04300 vs. NCBI nr
Match: KAG7011593.1 (Tubulin alpha chain [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 854 bits (2207), Expect = 3.05e-312
Identity = 429/451 (95.12%), Postives = 430/451 (95.34%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGI+PDGQMPGDTTVGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIEPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120
           HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD
Sbjct: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ------- 240
           VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ       
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQVISSLTA 240

Query: 241 --------------FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300
                         FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300

Query: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360
           MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP
Sbjct: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360

Query: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESDEGGDDDDQEEY 430
           AREDLAALEKDYEEVGAESDEGGDDDDQEEY
Sbjct: 421 AREDLAALEKDYEEVGAESDEGGDDDDQEEY 451

BLAST of Cp4.1LG15g04300 vs. NCBI nr
Match: XP_022972532.1 (tubulin alpha-4 chain-like [Cucurbita maxima])

HSP 1 Score: 852 bits (2200), Expect = 3.56e-311
Identity = 428/451 (94.90%), Postives = 429/451 (95.12%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMP DTTVGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDTTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120
           HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD
Sbjct: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ------- 240
           VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ       
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQVISSLTA 240

Query: 241 --------------FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300
                         FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300

Query: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360
           MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP
Sbjct: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360

Query: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESDEGGDDDDQEEY 430
           AREDLAALEKDYEEVGAESDEGG+DDDQEEY
Sbjct: 421 AREDLAALEKDYEEVGAESDEGGEDDDQEEY 451

BLAST of Cp4.1LG15g04300 vs. NCBI nr
Match: XP_008455054.1 (PREDICTED: tubulin alpha-4 chain [Cucumis melo] >KAA0031379.1 tubulin alpha-4 chain [Cucumis melo var. makuwa] >TYK06831.1 tubulin alpha-4 chain [Cucumis melo var. makuwa])

HSP 1 Score: 831 bits (2147), Expect = 4.25e-303
Identity = 417/451 (92.46%), Postives = 422/451 (93.57%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMP DTTVGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDTTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120
           HVPRA+FVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEI DLCLD
Sbjct: 61  HVPRAIFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ------- 240
           VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSL IERPTYTNLNRLISQ       
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLDIERPTYTNLNRLISQVISSLTA 240

Query: 241 --------------FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300
                         FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEIT+SAFEPSS
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360
           MMAKCDPR+GKYMACCLMYRGDVVPKD NAAVATIKTKRTIQFVDWCPTGFKCGINYQ P
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLA+VQRA+CMISNSTSVAEVF RIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESDEGGDDDDQEEY 430
           AREDLAALEKDYEEVGAES EG DDDDQEEY
Sbjct: 421 AREDLAALEKDYEEVGAESGEGDDDDDQEEY 451

BLAST of Cp4.1LG15g04300 vs. NCBI nr
Match: XP_038877541.1 (tubulin alpha-4 chain [Benincasa hispida])

HSP 1 Score: 826 bits (2134), Expect = 3.92e-301
Identity = 417/451 (92.46%), Postives = 422/451 (93.57%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMP DTTVGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDTTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120
           HVPRA+FVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEI DLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ------- 240
           VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSL IERPTYTNLNRLISQ       
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLDIERPTYTNLNRLISQVISSLTA 240

Query: 241 --------------FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300
                         FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEIT+SAFEPSS
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360
           MMAKCDPR+GKYMACCLMYRGDVVPKD NAAVATIKTKRTIQFVDWCPTGFKCGINYQ P
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLA+VQRA+CMISNSTSVAEVF RIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESDEGGDDDDQEEY 430
           AREDLAALEKDYEEVGAES EG DDDDQEEY
Sbjct: 421 AREDLAALEKDYEEVGAESAEG-DDDDQEEY 450

BLAST of Cp4.1LG15g04300 vs. ExPASy TrEMBL
Match: A0A6J1GJI6 (Tubulin alpha chain OS=Cucurbita moschata OX=3662 GN=LOC111454877 PE=3 SV=1)

HSP 1 Score: 855 bits (2210), Expect = 5.15e-313
Identity = 430/451 (95.34%), Postives = 430/451 (95.34%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120
           HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD
Sbjct: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ------- 240
           VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ       
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQVISSLTA 240

Query: 241 --------------FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300
                         FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300

Query: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360
           MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP
Sbjct: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360

Query: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESDEGGDDDDQEEY 430
           AREDLAALEKDYEEVGAESDEGGDDDDQEEY
Sbjct: 421 AREDLAALEKDYEEVGAESDEGGDDDDQEEY 451

BLAST of Cp4.1LG15g04300 vs. ExPASy TrEMBL
Match: A0A6J1I523 (Tubulin alpha chain OS=Cucurbita maxima OX=3661 GN=LOC111471076 PE=3 SV=1)

HSP 1 Score: 852 bits (2200), Expect = 1.72e-311
Identity = 428/451 (94.90%), Postives = 429/451 (95.12%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMP DTTVGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDTTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120
           HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD
Sbjct: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ------- 240
           VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ       
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQVISSLTA 240

Query: 241 --------------FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300
                         FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300

Query: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360
           MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP
Sbjct: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360

Query: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESDEGGDDDDQEEY 430
           AREDLAALEKDYEEVGAESDEGG+DDDQEEY
Sbjct: 421 AREDLAALEKDYEEVGAESDEGGEDDDQEEY 451

BLAST of Cp4.1LG15g04300 vs. ExPASy TrEMBL
Match: A0A5A7SPY9 (Tubulin alpha chain OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001350 PE=3 SV=1)

HSP 1 Score: 831 bits (2147), Expect = 2.06e-303
Identity = 417/451 (92.46%), Postives = 422/451 (93.57%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMP DTTVGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDTTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120
           HVPRA+FVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEI DLCLD
Sbjct: 61  HVPRAIFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ------- 240
           VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSL IERPTYTNLNRLISQ       
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLDIERPTYTNLNRLISQVISSLTA 240

Query: 241 --------------FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300
                         FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEIT+SAFEPSS
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360
           MMAKCDPR+GKYMACCLMYRGDVVPKD NAAVATIKTKRTIQFVDWCPTGFKCGINYQ P
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLA+VQRA+CMISNSTSVAEVF RIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESDEGGDDDDQEEY 430
           AREDLAALEKDYEEVGAES EG DDDDQEEY
Sbjct: 421 AREDLAALEKDYEEVGAESGEGDDDDDQEEY 451

BLAST of Cp4.1LG15g04300 vs. ExPASy TrEMBL
Match: A0A1S3C181 (Tubulin alpha chain OS=Cucumis melo OX=3656 GN=LOC103495324 PE=3 SV=1)

HSP 1 Score: 831 bits (2147), Expect = 2.06e-303
Identity = 417/451 (92.46%), Postives = 422/451 (93.57%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMP DTTVGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDTTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120
           HVPRA+FVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEI DLCLD
Sbjct: 61  HVPRAIFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ------- 240
           VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSL IERPTYTNLNRLISQ       
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLDIERPTYTNLNRLISQVISSLTA 240

Query: 241 --------------FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300
                         FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEIT+SAFEPSS
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360
           MMAKCDPR+GKYMACCLMYRGDVVPKD NAAVATIKTKRTIQFVDWCPTGFKCGINYQ P
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLA+VQRA+CMISNSTSVAEVF RIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESDEGGDDDDQEEY 430
           AREDLAALEKDYEEVGAES EG DDDDQEEY
Sbjct: 421 AREDLAALEKDYEEVGAESGEGDDDDDQEEY 451

BLAST of Cp4.1LG15g04300 vs. ExPASy TrEMBL
Match: A0A0A0K6A8 (Tubulin alpha chain OS=Cucumis sativus OX=3659 GN=Csa_7G070770 PE=3 SV=1)

HSP 1 Score: 824 bits (2129), Expect = 1.10e-300
Identity = 416/451 (92.24%), Postives = 421/451 (93.35%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMP D TVGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDKTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120
           HVPRA+FVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEI DLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ------- 240
           VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSL IERPTYTNLNRLISQ       
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLDIERPTYTNLNRLISQVISSLTA 240

Query: 241 --------------FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300
                         FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEIT+SAFEPSS
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360
           MMAKCDPR+GKYMACCLMYRGDVVPKD NAAVATIKTKRTIQFVDWCPTGFKCGINYQ P
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLA+VQRA+CMISNSTSVAEVF RIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESDEGGDDDDQEEY 430
           AREDLAALEKDYEEVGAES EG DDDDQEEY
Sbjct: 421 AREDLAALEKDYEEVGAESGEG-DDDDQEEY 450

BLAST of Cp4.1LG15g04300 vs. TAIR 10
Match: AT4G14960.2 (Tubulin/FtsZ family protein )

HSP 1 Score: 818.5 bits (2113), Expect = 2.5e-237
Identity = 406/449 (90.42%), Postives = 416/449 (92.65%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGD TVGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDKTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120
           HVPRA+FVDLEPTVIDEVRTG YRQLFHPEQLISGKEDAANNFARGHYTIGKEI DLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ------- 240
           VVEPYNSVLSTHSLLEHTDVS+LLDNEAIYDICRRSL IERPTYTNLNRL+SQ       
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVSILLDNEAIYDICRRSLNIERPTYTNLNRLVSQVISSLTA 240

Query: 241 --------------FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300
                         FQTNLVPYPRIHFMLSSYAPVISAEKA+HEQLSVAEIT+SAFEP+S
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAFHEQLSVAEITNSAFEPAS 300

Query: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360
           MMAKCDPR+GKYMACCLMYRGDVVPKD NAAV TIKTKRTIQFVDWCPTGFKCGINYQ P
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVGTIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLA+VQRA+CMISNSTSVAEVF RIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESDEGGDDDDQE 429
           AREDLAALEKDYEEVGA   EGGDD+D E
Sbjct: 421 AREDLAALEKDYEEVGA---EGGDDEDDE 446

BLAST of Cp4.1LG15g04300 vs. TAIR 10
Match: AT1G04820.1 (tubulin alpha-4 chain )

HSP 1 Score: 817.4 bits (2110), Expect = 5.5e-237
Identity = 405/449 (90.20%), Postives = 416/449 (92.65%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMP D TVGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDKTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120
           HVPRA+FVDLEPTVIDEVRTG YRQLFHPEQLISGKEDAANNFARGHYTIGKEI DLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ------- 240
           VVEPYNSVLSTHSLLEHTDVS+LLDNEAIYDICRRSL+IERPTYTNLNRL+SQ       
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVSILLDNEAIYDICRRSLSIERPTYTNLNRLVSQVISSLTA 240

Query: 241 --------------FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300
                         FQTNLVPYPRIHFMLSSYAPVISAEKA+HEQLSVAEIT+SAFEP+S
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAFHEQLSVAEITNSAFEPAS 300

Query: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360
           MMAKCDPR+GKYMACCLMYRGDVVPKD NAAV TIKTKRTIQFVDWCPTGFKCGINYQ P
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVGTIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLA+VQRA+CMISNSTSVAEVF RIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESDEGGDDDDQE 429
           AREDLAALEKDYEEVGA   EGGDD+D E
Sbjct: 421 AREDLAALEKDYEEVGA---EGGDDEDDE 446

BLAST of Cp4.1LG15g04300 vs. TAIR 10
Match: AT1G50010.1 (tubulin alpha-2 chain )

HSP 1 Score: 817.4 bits (2110), Expect = 5.5e-237
Identity = 405/449 (90.20%), Postives = 416/449 (92.65%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMP D TVGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDKTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120
           HVPRA+FVDLEPTVIDEVRTG YRQLFHPEQLISGKEDAANNFARGHYTIGKEI DLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ------- 240
           VVEPYNSVLSTHSLLEHTDVS+LLDNEAIYDICRRSL+IERPTYTNLNRL+SQ       
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVSILLDNEAIYDICRRSLSIERPTYTNLNRLVSQVISSLTA 240

Query: 241 --------------FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300
                         FQTNLVPYPRIHFMLSSYAPVISAEKA+HEQLSVAEIT+SAFEP+S
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAFHEQLSVAEITNSAFEPAS 300

Query: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360
           MMAKCDPR+GKYMACCLMYRGDVVPKD NAAV TIKTKRTIQFVDWCPTGFKCGINYQ P
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVGTIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLA+VQRA+CMISNSTSVAEVF RIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESDEGGDDDDQE 429
           AREDLAALEKDYEEVGA   EGGDD+D E
Sbjct: 421 AREDLAALEKDYEEVGA---EGGDDEDDE 446

BLAST of Cp4.1LG15g04300 vs. TAIR 10
Match: AT5G19770.1 (tubulin alpha-3 )

HSP 1 Score: 790.8 bits (2041), Expect = 5.5e-229
Identity = 390/449 (86.86%), Postives = 408/449 (90.87%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60
           MRE ISIHIGQAGIQVGN+CWELYCLEHGIQPDG MP DTTVG   DAFNTFFSETGAGK
Sbjct: 1   MREIISIHIGQAGIQVGNSCWELYCLEHGIQPDGMMPSDTTVGVAHDAFNTFFSETGAGK 60

Query: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120
           HVPRA+FVDLEPTVIDEVRTG YRQLFHPEQLISGKEDAANNFARGHYT+GKEI DLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           R+RKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFT+YPSPQVST+
Sbjct: 121 RVRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTIYPSPQVSTA 180

Query: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ------- 240
           VVEPYNSVLSTHSLLEHTDV+VLLDNEAIYDICRRSL IERPTYTNLNRLISQ       
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPTYTNLNRLISQIISSLTT 240

Query: 241 --------------FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300
                         FQTNLVPYPRIHFMLSSYAPVISA KAYHEQLSV EIT++ FEP+S
Sbjct: 241 SLRFDGAINVDITEFQTNLVPYPRIHFMLSSYAPVISAAKAYHEQLSVPEITNAVFEPAS 300

Query: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360
           MMAKCDPR+GKYMACCLMYRGDVVPKD NAAV TIKTKRT+QFVDWCPTGFKCGINYQ P
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVGTIKTKRTVQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLA+VQRA+CMISN+T+VAEVF RIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLAKVQRAVCMISNNTAVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESDEGGDDDDQE 429
           AREDLAALEKDYEEVGA   EGGDD++ E
Sbjct: 421 AREDLAALEKDYEEVGA---EGGDDEEDE 446

BLAST of Cp4.1LG15g04300 vs. TAIR 10
Match: AT5G19780.1 (tubulin alpha-5 )

HSP 1 Score: 790.8 bits (2041), Expect = 5.5e-229
Identity = 390/449 (86.86%), Postives = 408/449 (90.87%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTVGGGDDAFNTFFSETGAGK 60
           MRE ISIHIGQAGIQVGN+CWELYCLEHGIQPDG MP DTTVG   DAFNTFFSETGAGK
Sbjct: 1   MREIISIHIGQAGIQVGNSCWELYCLEHGIQPDGMMPSDTTVGVAHDAFNTFFSETGAGK 60

Query: 61  HVPRALFVDLEPTVIDEVRTGMYRQLFHPEQLISGKEDAANNFARGHYTIGKEIADLCLD 120
           HVPRA+FVDLEPTVIDEVRTG YRQLFHPEQLISGKEDAANNFARGHYT+GKEI DLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           R+RKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFT+YPSPQVST+
Sbjct: 121 RVRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTIYPSPQVSTA 180

Query: 181 VVEPYNSVLSTHSLLEHTDVSVLLDNEAIYDICRRSLAIERPTYTNLNRLISQ------- 240
           VVEPYNSVLSTHSLLEHTDV+VLLDNEAIYDICRRSL IERPTYTNLNRLISQ       
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPTYTNLNRLISQIISSLTT 240

Query: 241 --------------FQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITSSAFEPSS 300
                         FQTNLVPYPRIHFMLSSYAPVISA KAYHEQLSV EIT++ FEP+S
Sbjct: 241 SLRFDGAINVDITEFQTNLVPYPRIHFMLSSYAPVISAAKAYHEQLSVPEITNAVFEPAS 300

Query: 301 MMAKCDPRNGKYMACCLMYRGDVVPKDANAAVATIKTKRTIQFVDWCPTGFKCGINYQAP 360
           MMAKCDPR+GKYMACCLMYRGDVVPKD NAAV TIKTKRT+QFVDWCPTGFKCGINYQ P
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVGTIKTKRTVQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAICMISNSTSVAEVFCRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLA+VQRA+CMISN+T+VAEVF RIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLAKVQRAVCMISNNTAVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESDEGGDDDDQE 429
           AREDLAALEKDYEEVGA   EGGDD++ E
Sbjct: 421 AREDLAALEKDYEEVGA---EGGDDEEDE 446

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZRR52.4e-23790.47Tubulin alpha-3 chain OS=Hordeum vulgare OX=4513 GN=TUBA3 PE=2 SV=1[more]
P336294.1e-23790.87Tubulin alpha chain OS=Prunus dulcis OX=3755 GN=TUBA PE=2 SV=1[more]
P462595.3e-23790.71Tubulin alpha-1 chain OS=Pisum sativum OX=3888 GN=TUBA1 PE=3 SV=1[more]
Q6VAF99.1e-23790.69Tubulin alpha-4 chain OS=Gossypium hirsutum OX=3635 PE=2 SV=1[more]
Q964601.6e-23690.02Tubulin alpha-2 chain OS=Hordeum vulgare OX=4513 GN=TUBA2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022952112.11.06e-31295.34tubulin alpha chain-like [Cucurbita moschata] >XP_023553814.1 tubulin alpha chai... [more]
KAG7011593.13.05e-31295.12Tubulin alpha chain [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022972532.13.56e-31194.90tubulin alpha-4 chain-like [Cucurbita maxima][more]
XP_008455054.14.25e-30392.46PREDICTED: tubulin alpha-4 chain [Cucumis melo] >KAA0031379.1 tubulin alpha-4 ch... [more]
XP_038877541.13.92e-30192.46tubulin alpha-4 chain [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1GJI65.15e-31395.34Tubulin alpha chain OS=Cucurbita moschata OX=3662 GN=LOC111454877 PE=3 SV=1[more]
A0A6J1I5231.72e-31194.90Tubulin alpha chain OS=Cucurbita maxima OX=3661 GN=LOC111471076 PE=3 SV=1[more]
A0A5A7SPY92.06e-30392.46Tubulin alpha chain OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G0... [more]
A0A1S3C1812.06e-30392.46Tubulin alpha chain OS=Cucumis melo OX=3656 GN=LOC103495324 PE=3 SV=1[more]
A0A0A0K6A81.10e-30092.24Tubulin alpha chain OS=Cucumis sativus OX=3659 GN=Csa_7G070770 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G14960.22.5e-23790.42Tubulin/FtsZ family protein [more]
AT1G04820.15.5e-23790.20tubulin alpha-4 chain [more]
AT1G50010.15.5e-23790.20tubulin alpha-2 chain [more]
AT5G19770.15.5e-22986.86tubulin alpha-3 [more]
AT5G19780.15.5e-22986.86tubulin alpha-5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 397..417
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 410..430
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 413..430
NoneNo IPR availablePANTHERPTHR11588:SF405TUBULIN ALPHA CHAINcoord: 1..233
NoneNo IPR availablePANTHERPTHR11588:SF405TUBULIN ALPHA CHAINcoord: 233..428
NoneNo IPR availableCDDcd02186alpha_tubulincoord: 2..414
e-value: 0.0
score: 832.184
IPR000217TubulinPRINTSPR01161TUBULINcoord: 108..132
score: 58.13
coord: 178..191
score: 70.91
coord: 53..72
score: 65.51
coord: 10..30
score: 66.54
coord: 95..106
score: 64.97
coord: 359..387
score: 56.79
coord: 192..212
score: 60.52
coord: 134..152
score: 66.84
coord: 153..174
score: 49.68
IPR000217TubulinPANTHERPTHR11588TUBULINcoord: 1..233
IPR000217TubulinPANTHERPTHR11588TUBULINcoord: 233..428
IPR002452Alpha tubulinPRINTSPR01162ALPHATUBULINcoord: 339..358
score: 79.83
coord: 18..33
score: 95.27
coord: 364..380
score: 80.13
coord: 120..128
score: 86.55
coord: 88..101
score: 94.12
coord: 161..172
score: 83.82
coord: 148..160
score: 86.23
coord: 326..339
score: 86.61
coord: 214..226
score: 79.57
coord: 401..417
score: 90.81
coord: 45..58
score: 85.23
coord: 300..321
score: 86.59
IPR018316Tubulin/FtsZ, 2-layer sandwich domainSMARTSM00865Tubulin_C_4coord: 227..372
e-value: 8.8E-43
score: 158.1
IPR018316Tubulin/FtsZ, 2-layer sandwich domainPFAMPF03953Tubulin_Ccoord: 242..371
e-value: 4.0E-49
score: 166.0
IPR003008Tubulin/FtsZ, GTPase domainSMARTSM00864Tubulin_4coord: 49..243
e-value: 1.5E-60
score: 217.2
IPR003008Tubulin/FtsZ, GTPase domainPFAMPF00091Tubulincoord: 3..213
e-value: 1.0E-67
score: 228.2
IPR036525Tubulin/FtsZ, GTPase domain superfamilyGENE3D3.40.50.1440Tubulin/FtsZ, GTPase domaincoord: 1..247
e-value: 2.0E-115
score: 386.8
IPR036525Tubulin/FtsZ, GTPase domain superfamilySUPERFAMILY52490Tubulin nucleotide-binding domain-likecoord: 1..239
IPR037103Tubulin/FtsZ-like, C-terminal domainGENE3D3.30.1330.20coord: 248..362
e-value: 1.7E-57
score: 194.2
IPR023123Tubulin, C-terminalGENE3D1.10.287.600Helix hairpin bincoord: 363..415
e-value: 6.7E-31
score: 107.7
IPR017975Tubulin, conserved sitePROSITEPS00227TUBULINcoord: 142..148
IPR008280Tubulin/FtsZ, C-terminalSUPERFAMILY55307Tubulin C-terminal domain-likecoord: 231..415

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG15g04300.1Cp4.1LG15g04300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000226 microtubule cytoskeleton organization
biological_process GO:0000278 mitotic cell cycle
biological_process GO:0007017 microtubule-based process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005874 microtubule
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0005200 structural constituent of cytoskeleton