Cp4.1LG15g03220 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG15g03220
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	membrane steroid-binding protein 2-like
Location	Cp4.1LG15: 4256949 .. 4260165 (-)
RNA-Seq Expression	Cp4.1LG15g03220
Synteny	Cp4.1LG15g03220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCGCAGATGTTAAATGTTGTCCACGAACAACGGTGACCCTTGATTAAAAACCTTTAGGTTGGTAACAAAAAGCTGCTGCCCTTTTCCTCTCTCATTCTCCTCGGTTTCTCTCTCTCTCTCTCTCTCCCCCTCTGCCATGGCTCTGCAAGTCTGGGAGACGTTGAAGGAGGCGATCGTGGCCTACACAGGCCTCTCTCCGTCGACCTTCTTCACAGTCTTGGCTCTTGGATTGGCCTTTTACTATCTCATTTCCAGTTTCTTCGGTCCCTCCGACTACGGAACGCATACTAGGGACTTAGGGGAGCAGCTCCAGCCTCTCCCTCCGCCTGTCCAAGTCGGTGAGGTTTCTGAGGATGAGTTGAAGCAATACGATGGCTCCGATCCTGAGAAGCCTCTGCTCATGGCTATCAAGGGCCAGATCTACGATGTTTCTCAGAGCAGGTCGGTTGAGAATTTTCTTTTATTGAAAAATTTCCCCTCTGTTTTAGCTATTTAGTGATTGGATGATCGTGATGTGTTTTGGTTCAGTTTCAATTAGTTCTTGTGTCAACGTCTTTGTTCAATTTTGTGATGGTAGGTGGAATGTTAGCCGGTTTTAGGTTTACAAAAGCTTTTTATATTTTATTTCAGTTTTTTGAAGTAATTGATTTCGTGCTTCGTTTTGCCTTCTACAGCTCTCCTTTTCGTTCAAAGAGCTTACTGATTCCCGCAAAATGAAACTTGATATGATCCTACTGTANTTGTTTTTTTTTTTTTTTTTTTTGGCTATTTCTAATTCGTCCTCTCTTCCTGTTTCTTTTGTCAAGAATCATTCATTGTCCACTATACATCCCCTATCTTTTATGGTGTTCTATGTTTGTCATAATATTTGCTAGGACAACCAAAGAAGAACTTATTGATTTTTTGAGTTCTGGACTTCAATTCAAACTTCGAATCTTTTTCGTGTTGGACCAGGAAACCTCTCAAAATAGATTGGACCAGAAATTAATCTTCTTGTTTTTGTGCATCTGACTTTTAATTTTGATGTTTATTGTTCCTTCTTATAGCTGTAATTTGCTACATATCTGGTTTTGAAAATATGTAATTGGGACTGTCGTTTTTTGCTTTCAAGTTACGGCCATGGTATTTCCAGCATATAGAATTCTTATAGCATGTGGAGTTCACAAATGCCAGAGTTTGATGTTCCACGAGTTATTGTGAAAACTTGGGGCTGAAGAAGGTTCTGTTAAATGTAAAATCACTTTTAAACGAGCTAATGATTTCAAATCACTTTTTACAAACTTATGATTTGAAAGAGAATAAGTATAAGAGAAAACAATGTAGAAAGTAGTTCTCATTATGCGCTAACAATTAGAAAATTGCTTTTCCAAAGACTTTATAAGAACAGGTCCTTTGTTTCGTGGTTAGGGTATACTTTTGAACCGTTCTTTGCATGATGTGTTTGTTAGAAATGGCCCACGATCTAAATATCTCAAAACAAAGAAAGGGAGCAAAAAGAGGAGAGAGCTTTTGGTACAAGGAACCTTAAGCACTTAACTTTTCAATTTTCTTTGTTGTTATGAAAAAAACATACATCTCGGGAGTACTTATAGTGTACTTACAGTGGAGAAAGCACTGATTTGCTTCCTAAACTTTCTCTTCGCACTAACAATCTTCCTAGATAAATCATAAAACTTGGTCCACAATTTGGCGGATTCATTTTGATTAAGCTTCCTTCCTATTAATTTGGAAGTTCTTCTCAGATACAAGGAAATTATTCAAGGAAATCATTCAGTTCCAAAGTCAAAGATCCTAATTCTCGAACGAGCAATTGAAAAGATTCTGGAGTATCCTCAGGTTTAGTTATTGTTCCTCCAATGATCGTAGTAACAAGTAACTACTACCAATTCTCCTATATTGGTCCATAAGTTAGTAACTTATTCTACTAAATCCACTAACAATTCTCTTAAAGTTACTCCATAAGTTAGTGACTTATTCTACTAAGTCAAGTATATAAGCGCATAGTGGTATTTACTACACTAAGGTTGTAGAAACGAAGATCTGTTATGTTTAATGACTTATTCTACTTAGTCAAGTTTACAAACTCATAGTGTTATTTACTACACTAAAGTTGTAGACACGAAGATCTGTTTATGTCTCCTGTTTCTTTCCCGTGTCTTGTTCTCTTGTGTGTGCGTACTCGTAAAGGAAAACTAAAATTGAAGAAAATATACGCCTACATATATTGCTTCAATTTTGACCCTCTTTGGTTGCAGGATGTTCTATGGACCTGGAGGGCCGTATGCCCTTTTTGCTGGAAAGGATGCCAGCAGAGCTCTTGCAAAAATGTCGTTTGAAGAGAAAGATCTGACAGGGGATATCTCTGGTCTTGGGCCATTTGAACTGGAAGCCTTACAGGACTGGGAATACAAATTCATGAGTAAATATGTTAAAGTTGGGACAATCAAGAAGCCAGTTGCTGATGGTGATGCTACGCCGGCTGCTGGTGAGCCTGCAGAATCAAGCACAGCAGAAGTTAGCAGCAGCAGCAAGCCTGCTGAAGACAGTCCCTCGGAATCTCATGCTGATAGAGCCGCCGAGGAAACCCCTGCTGCTGCAAAAAACGTTGAATAATATTTTTGTGAGTGGAGAACGATGTGTACGTGTGGTCATGTTTCAGTAATCTTGTTTTATAGATAAGTGAAGTGTAGGTGTGTAGGAAGGTATTACAGGTGGAGTTAAGACTTAGAGTTTATGTTACTCTTGTTCAACAAACATCTGACAATAAGATAAGTCTTTGAATTTGGAAATAGGACTCAGGTCTCTCTTTCCCCAATTTGTTTGACCTGATTGGATTGAAACTGAAAGTATGAACTCATGAATATAAATAAATAGGGGATTAGCAATTCCATTGGGTTTGAGGCCTCCATTAATATGTATATAGCTTTTAGTTTGATAGGGGGATTCGAAGTTTTGATCTCGTATCAAAGAAATATCATATTTTGAAAATGTCATTTGTACCGACTTTGTAGGTGAGGTTACACATGCTAAGTACTCACTAAAATTGTTAGGGAAGTTACATTAATGTTATTTCAATGGAAACTTTTATTTAAAAAAAAAAAAAAAAAGTTGGTAATCAATTAAATCATGTTGATGTTTTTAAATGCTAAATGATACAATGTTGTTGATGTACAATGTTGCTGTGGTGATGAGCTGATATGCTAAGCTGTGTGATGAG

mRNA sequence

GCGCAGATGTTAAATGTTGTCCACGAACAACGGTGACCCTTGATTAAAAACCTTTAGGTTGGTAACAAAAAGCTGCTGCCCTTTTCCTCTCTCATTCTCCTCGGTTTCTCTCTCTCTCTCTCTCTCCCCCTCTGCCATGGCTCTGCAAGTCTGGGAGACGTTGAAGGAGGCGATCGTGGCCTACACAGGCCTCTCTCCGTCGACCTTCTTCACAGTCTTGGCTCTTGGATTGGCCTTTTACTATCTCATTTCCAGTTTCTTCGGTCCCTCCGACTACGGAACGCATACTAGGGACTTAGGGGAGCAGCTCCAGCCTCTCCCTCCGCCTGTCCAAGTCGGTGAGGTTTCTGAGGATGAGTTGAAGCAATACGATGGCTCCGATCCTGAGAAGCCTCTGCTCATGGCTATCAAGGGCCAGATCTACGATGTTTCTCAGAGCAGGATGTTCTATGGACCTGGAGGGCCGTATGCCCTTTTTGCTGGAAAGGATGCCAGCAGAGCTCTTGCAAAAATGTCGTTTGAAGAGAAAGATCTGACAGGGGATATCTCTGGTCTTGGGCCATTTGAACTGGAAGCCTTACAGGACTGGGAATACAAATTCATGAGTAAATATGTTAAAGTTGGGACAATCAAGAAGCCAGTTGCTGATGGTGATGCTACGCCGGCTGCTGGTGAGCCTGCAGAATCAAGCACAGCAGAAGTTAGCAGCAGCAGCAAGCCTGCTGAAGACAGTCCCTCGGAATCTCATGCTGATAGAGCCGCCGAGGAAACCCCTGCTGCTGCAAAAAACGTTGAATAATATTTTTGTGAGTGGAGAACGATGTGTACGTGTGGTCATGTTTCAGTAATCTTGTTTTATAGATAAGTGAAGTGTAGGTGTGTAGGAAGGTATTACAGGTGGAGTTAAGACTTAGAGTTTATGTTACTCTTGTTCAACAAACATCTGACAATAAGATAAGTCTTTGAATTTGGAAATAGGACTCAGGTCTCTCTTTCCCCAATTTGTTTGACCTGATTGGATTGAAACTGAAAGTATGAACTCATGAATATAAATAAATAGGGGATTAGCAATTCCATTGGGTTTGAGGCCTCCATTAATATGTATATAGCTTTTAGTTTGATAGGGGGATTCGAAGTTTTGATCTCGTATCAAAGAAATATCATATTTTGAAAATGTCATTTGTACCGACTTTGTAGGTGAGGTTACACATGCTAAGTACTCACTAAAATTGTTAGGGAAGTTACATTAATGTTATTTCAATGGAAACTTTTATTTAAAAAAAAAAAAAAAAAGTTGGTAATCAATTAAATCATGTTGATGTTTTTAAATGCTAAATGATACAATGTTGTTGATGTACAATGTTGCTGTGGTGATGAGCTGATATGCTAAGCTGTGTGATGAG

Coding sequence (CDS)

ATGGCTCTGCAAGTCTGGGAGACGTTGAAGGAGGCGATCGTGGCCTACACAGGCCTCTCTCCGTCGACCTTCTTCACAGTCTTGGCTCTTGGATTGGCCTTTTACTATCTCATTTCCAGTTTCTTCGGTCCCTCCGACTACGGAACGCATACTAGGGACTTAGGGGAGCAGCTCCAGCCTCTCCCTCCGCCTGTCCAAGTCGGTGAGGTTTCTGAGGATGAGTTGAAGCAATACGATGGCTCCGATCCTGAGAAGCCTCTGCTCATGGCTATCAAGGGCCAGATCTACGATGTTTCTCAGAGCAGGATGTTCTATGGACCTGGAGGGCCGTATGCCCTTTTTGCTGGAAAGGATGCCAGCAGAGCTCTTGCAAAAATGTCGTTTGAAGAGAAAGATCTGACAGGGGATATCTCTGGTCTTGGGCCATTTGAACTGGAAGCCTTACAGGACTGGGAATACAAATTCATGAGTAAATATGTTAAAGTTGGGACAATCAAGAAGCCAGTTGCTGATGGTGATGCTACGCCGGCTGCTGGTGAGCCTGCAGAATCAAGCACAGCAGAAGTTAGCAGCAGCAGCAAGCCTGCTGAAGACAGTCCCTCGGAATCTCATGCTGATAGAGCCGCCGAGGAAACCCCTGCTGCTGCAAAAAACGTTGAATAA

Protein sequence

MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQPLPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVADGDATPAAGEPAESSTAEVSSSSKPAEDSPSESHADRAAEETPAAAKNVE

Homology

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BLAST of Cp4.1LG15g03220 vs. ExPASy Swiss-Prot
Match: Q9M2Z4 (Membrane steroid-binding protein 2 OS=Arabidopsis thaliana OX=3702 GN=MSBP2 PE=1 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 1.6e-67
Identity = 140/221 (63.35%), Postives = 164/221 (74.21%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFF-GPSDYGTHTRDLGEQLQ 60
           M  Q+WETLKE I AYTGLSP+ FFTVLAL  A Y ++S FF  P  +   + ++  Q +
Sbjct: 1   MVQQIWETLKETITAYTGLSPAAFFTVLALAFAVYQVVSGFFVSPEVHRPRSLEVQPQSE 60

Query: 61  PLPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120
           PLPPPVQ+GE++E+ELK YDGSD +KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA
Sbjct: 61  PLPPPVQLGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120

Query: 121 SRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVADGDATPAAG 180
           SRALAKMSFE++DLTGDISGLG FELEALQDWEYKFMSKYVKVGTI+K   +G     + 
Sbjct: 121 SRALAKMSFEDQDLTGDISGLGAFELEALQDWEYKFMSKYVKVGTIQKKDGEG---KESS 180

Query: 181 EPAESSTAEV--------SSSSKPAEDSPSESHADRAAEET 213
           EP+E+ TA            +S    D  S S  ++ AE T
Sbjct: 181 EPSEAKTASAEGLSTNTGEEASAITHDETSRSTGEKIAETT 218

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BLAST of Cp4.1LG15g03220 vs. ExPASy Swiss-Prot
Match: Q9FVZ7 (Membrane steroid-binding protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MSBP1 PE=1 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 6.0e-67
Identity = 137/216 (63.43%), Postives = 162/216 (75.00%), Query Frame = 0


Query: 4   QVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFG--PSDYGTHTRDLGEQLQPL 63
           ++WETLK+AIVAYTGLSP+ FFT +A   A Y+++S  F   P       RD  E  +PL
Sbjct: 7   ELWETLKQAIVAYTGLSPAAFFTAVAAAAALYHVVSGIFAGPPPPPPPRPRDEPE-AEPL 66

Query: 64  PPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASR 123
           PPPVQ+GEVSE+EL+QYDGSDP+KPLLMAIKGQIYDV+QSRMFYGPGGPYALFAGKDASR
Sbjct: 67  PPPVQLGEVSEEELRQYDGSDPKKPLLMAIKGQIYDVTQSRMFYGPGGPYALFAGKDASR 126

Query: 124 ALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVADGDATPAAG-E 183
           ALAKMSFE +DLTGDISGLGPFEL+ALQDWEYKFM KYVKVGT+KK V   D  P+   E
Sbjct: 127 ALAKMSFEPQDLTGDISGLGPFELDALQDWEYKFMGKYVKVGTVKKTVPVEDGAPSTSPE 186

Query: 184 PAESSTAEVSSSSKPAEDSPSESHADRAAEETPAAA 217
             E++ A     +   E+ P E  ++   E+  A A
Sbjct: 187 TTETAAAAEPEKAPATEEKPREVSSEEVKEKEDAVA 221

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BLAST of Cp4.1LG15g03220 vs. ExPASy Swiss-Prot
Match: Q9XFM6 (Membrane steroid-binding protein 1 OS=Arabidopsis thaliana OX=3702 GN=MSBP1 PE=1 SV=2)

HSP 1 Score: 248.4 bits (633), Expect = 7.3e-65
Identity = 134/215 (62.33%), Postives = 162/215 (75.35%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGP--SDYGTHTRDLG--- 60
           MAL++W+TLKEAI AYTGLSP  FFT LAL  A Y +IS +F     D   H R      
Sbjct: 1   MALELWQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFDDVNRHQRARSLAQ 60

Query: 61  EQLQPLPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFA 120
           E+  P+P PVQVGE++E+ELKQYDGSDP+KPLLMAIK QIYDV+QSRMFYGPGGPYALFA
Sbjct: 61  EEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPYALFA 120

Query: 121 GKDASRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVADGDAT 180
           GKDASRALAKMSFEEKDLT D+SGLGPFEL+ALQDWEYKFMSKY KVGT+K   ++ + T
Sbjct: 121 GKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVGTVKVAGSEPE-T 180

Query: 181 PAAGEPAESSTAEVSSSSKP-------AEDSPSES 204
            +  EP E+   +   ++ P       ++D+P+E+
Sbjct: 181 ASVSEPTENVEQDAHVTTTPGKTVVDKSDDAPAET 214

[Close]

BLAST of Cp4.1LG15g03220 vs. ExPASy Swiss-Prot
Match: Q9FVZ9 (Membrane steroid-binding protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=MSBP2 PE=2 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 9.6e-49
Identity = 109/175 (62.29%), Postives = 121/175 (69.14%), Query Frame = 0


Query: 11  EAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTR-DLGEQLQPLPPPVQVGE 70
           E   A    SP      +A     Y + SS   P       R +  E+  P P PVQVGE
Sbjct: 61  ELWAAARSASPVAVIAAVAGAAVVYKVGSSLLAPPPPPARPREEPSEEAPPPPEPVQVGE 120

Query: 71  VSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSFE 130
           ++ +EL QYDGSDPEKPLLMAIKGQIYDVSQSR+FYGPGGPYALFAGKDASRALAKMSFE
Sbjct: 121 ITAEELLQYDGSDPEKPLLMAIKGQIYDVSQSRLFYGPGGPYALFAGKDASRALAKMSFE 180

Query: 131 EKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAD--GDATPAAGEPA 183
            +DLT DISGL   EL ALQDWEYKF SKYVKVGTIKK + +  GD+T  A E A
Sbjct: 181 PQDLTDDISGLSLLELSALQDWEYKFSSKYVKVGTIKKVLVEQGGDSTADAIEEA 235

[Close]

BLAST of Cp4.1LG15g03220 vs. ExPASy Swiss-Prot
Match: Q9SK39 (Probable steroid-binding protein 3 OS=Arabidopsis thaliana OX=3702 GN=MP3 PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 9.0e-23
Identity = 48/97 (49.48%), Postives = 69/97 (71.13%), Query Frame = 0


Query: 69  EVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSF 128
           E + ++L QY+G+D  KP+ +AIKG+++DV+  + FYG GG Y++FAGKDASRAL KMS 
Sbjct: 2   EFTAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSK 61

Query: 129 EEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTI 166
            E+D++  + GL   E+  L DWE KF +KY  VG +
Sbjct: 62  NEEDVSPSLEGLTEKEINTLNDWETKFEAKYPVVGRV 98

[Close]

BLAST of Cp4.1LG15g03220 vs. NCBI nr
Match: XP_023554188.1 (membrane steroid-binding protein 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 425 bits (1093), Expect = 7.35e-150
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60
           MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60

Query: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVADGDATPAAGE 180
           RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVADGDATPAAGE
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVADGDATPAAGE 180

Query: 181 PAESSTAEVSSSSKPAEDSPSESHADRAAEETPAAAKNVE 220
           PAESSTAEVSSSSKPAEDSPSESHADRAAEETPAAAKNVE
Sbjct: 181 PAESSTAEVSSSSKPAEDSPSESHADRAAEETPAAAKNVE 220

[Close]

BLAST of Cp4.1LG15g03220 vs. NCBI nr
Match: XP_022952639.1 (membrane steroid-binding protein 2-like [Cucurbita moschata] >XP_022952640.1 membrane steroid-binding protein 2-like [Cucurbita moschata])

HSP 1 Score: 416 bits (1069), Expect = 3.48e-146
Identity = 217/221 (98.19%), Postives = 219/221 (99.10%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60
           MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60

Query: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVADGDATPAAGE 180
           RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVA+GDA PAAGE
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDAAPAAGE 180

Query: 181 PAESSTAEVSSSS-KPAEDSPSESHADRAAEETPAAAKNVE 220
           PAESSTAEVSSSS KPAEDSPSESHAD+AAEETPAAAKNVE
Sbjct: 181 PAESSTAEVSSSSSKPAEDSPSESHADKAAEETPAAAKNVE 221

[Close]

BLAST of Cp4.1LG15g03220 vs. NCBI nr
Match: XP_022971922.1 (membrane steroid-binding protein 2-like [Cucurbita maxima])

HSP 1 Score: 414 bits (1065), Expect = 1.32e-145
Identity = 215/220 (97.73%), Postives = 218/220 (99.09%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60
           MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60

Query: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQ+GEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQIGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVADGDATPAAGE 180
           RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVA+GDA PAAGE
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDAAPAAGE 180

Query: 181 PAESSTAEVSSSSKPAEDSPSESHADRAAEETPAAAKNVE 220
           PAESSTAEVSSS KPAEDSPSESHAD+AAEETPAAAKNVE
Sbjct: 181 PAESSTAEVSSS-KPAEDSPSESHADKAAEETPAAAKNVE 219

[Close]

BLAST of Cp4.1LG15g03220 vs. NCBI nr
Match: KAG6571809.1 (Membrane steroid-binding protein 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011504.1 Membrane steroid-binding protein 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 414 bits (1065), Expect = 1.42e-145
Identity = 216/221 (97.74%), Postives = 218/221 (98.64%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60
           MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60

Query: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVADGDATPAAGE 180
           RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVA+GD  PAAGE
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDTAPAAGE 180

Query: 181 PAESSTAEVSSSS-KPAEDSPSESHADRAAEETPAAAKNVE 220
           PAESSTAEVSSSS KPAEDSPSESHAD+AAEETPAAAKNVE
Sbjct: 181 PAESSTAEVSSSSSKPAEDSPSESHADKAAEETPAAAKNVE 221

[Close]

BLAST of Cp4.1LG15g03220 vs. NCBI nr
Match: XP_038888418.1 (membrane steroid-binding protein 2-like [Benincasa hispida])

HSP 1 Score: 363 bits (933), Expect = 1.40e-125
Identity = 193/216 (89.35%), Postives = 202/216 (93.52%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60
           MALQVWETLKEAIVAYTGLSP+TFFTVLALG+A YYLISSFF PSDYGTHTRDLGEQLQP
Sbjct: 1   MALQVWETLKEAIVAYTGLSPATFFTVLALGIAIYYLISSFFAPSDYGTHTRDLGEQLQP 60

Query: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQVGE+SED+LKQYDGSDP+KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQVGEISEDDLKQYDGSDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVADGDATPAAGE 180
           RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGT++K VADGDA  AAGE
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTVQKSVADGDA--AAGE 180

Query: 181 PAESSTAEVSSSSKPAEDSPSESHADRAAEETPAAA 216
           PAESS AEVS   KPAE+S SES  D+  EETPAAA
Sbjct: 181 PAESSIAEVS---KPAEESHSESRDDKV-EETPAAA 210

[Close]

BLAST of Cp4.1LG15g03220 vs. ExPASy TrEMBL
Match: A0A6J1GMC2 (membrane steroid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111455271 PE=4 SV=1)

HSP 1 Score: 416 bits (1069), Expect = 1.68e-146
Identity = 217/221 (98.19%), Postives = 219/221 (99.10%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60
           MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60

Query: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVADGDATPAAGE 180
           RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVA+GDA PAAGE
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDAAPAAGE 180

Query: 181 PAESSTAEVSSSS-KPAEDSPSESHADRAAEETPAAAKNVE 220
           PAESSTAEVSSSS KPAEDSPSESHAD+AAEETPAAAKNVE
Sbjct: 181 PAESSTAEVSSSSSKPAEDSPSESHADKAAEETPAAAKNVE 221

[Close]

BLAST of Cp4.1LG15g03220 vs. ExPASy TrEMBL
Match: A0A6J1I394 (membrane steroid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111470596 PE=4 SV=1)

HSP 1 Score: 414 bits (1065), Expect = 6.38e-146
Identity = 215/220 (97.73%), Postives = 218/220 (99.09%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60
           MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60

Query: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQ+GEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQIGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVADGDATPAAGE 180
           RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVA+GDA PAAGE
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDAAPAAGE 180

Query: 181 PAESSTAEVSSSSKPAEDSPSESHADRAAEETPAAAKNVE 220
           PAESSTAEVSSS KPAEDSPSESHAD+AAEETPAAAKNVE
Sbjct: 181 PAESSTAEVSSS-KPAEDSPSESHADKAAEETPAAAKNVE 219

[Close]

BLAST of Cp4.1LG15g03220 vs. ExPASy TrEMBL
Match: A0A6J1GZX3 (membrane steroid-binding protein 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459181 PE=4 SV=1)

HSP 1 Score: 353 bits (905), Expect = 1.44e-121
Identity = 190/218 (87.16%), Postives = 199/218 (91.28%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60
           MALQVWETLKEAIVAYTGLSPSTFFT +A GLAFY+LISS F PSD  THTRDLGEQLQP
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTAVAFGLAFYFLISSLFAPSDNRTHTRDLGEQLQP 60

Query: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQVGE+SE+ELKQYDGSD EKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQVGEISEEELKQYDGSDSEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVADG-DATPAAG 180
           RALAKMSFEEKDLTGDISGLGPFEL+ALQDWEYKFMSKYVKVGT+KKPVA+G DA P   
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTVKKPVAEGGDAAPVVV 180

Query: 181 EPAESSTAEVSSSSKPAEDS-PSESHADRAAEETPAAA 216
           EPAESSTAEVS   KPAE+  PSES AD+A EETPAAA
Sbjct: 181 EPAESSTAEVS---KPAEEERPSESRADKA-EETPAAA 214

[Close]

BLAST of Cp4.1LG15g03220 vs. ExPASy TrEMBL
Match: A0A6J1K773 (membrane steroid-binding protein 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490814 PE=4 SV=1)

HSP 1 Score: 351 bits (900), Expect = 8.29e-121
Identity = 191/222 (86.04%), Postives = 201/222 (90.54%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60
           MALQVWETLKEAI+AYTGLSPSTFFT +A GLAFY+LIS FF PSD  THTRDLGEQLQP
Sbjct: 1   MALQVWETLKEAILAYTGLSPSTFFTAVAFGLAFYFLISGFFAPSDNRTHTRDLGEQLQP 60

Query: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQVGE+SE+ELKQYDGSD  KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQVGEISEEELKQYDGSDSGKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVADG-DATPAAG 180
           RALAKMSFEEKDLTGDISGLGPFEL+ALQDWEYKFMSKYVKVGT+KKPVA+G DA PA  
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTVKKPVAEGGDAAPAVV 180

Query: 181 EPAESSTAEVSSSSKPAEDS-PSESHADRAAEETPAAAKNVE 220
           EPAESSTAEVS   KPAE+  PSES AD+A EETPA A NVE
Sbjct: 181 EPAESSTAEVS---KPAEEERPSESRADKA-EETPAVA-NVE 217

[Close]

BLAST of Cp4.1LG15g03220 vs. ExPASy TrEMBL
Match: A0A6J1E0F8 (membrane steroid-binding protein 2-like OS=Momordica charantia OX=3673 GN=LOC111026011 PE=4 SV=1)

HSP 1 Score: 339 bits (869), Expect = 4.37e-116
Identity = 183/223 (82.06%), Postives = 199/223 (89.24%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60
           MALQVWETLKEAIVAYTGLSP+TFFTVLALGLA YY+IS FF  SD G+ +RD+GE LQP
Sbjct: 1   MALQVWETLKEAIVAYTGLSPTTFFTVLALGLAIYYVISGFFASSDAGSRSRDIGEPLQP 60

Query: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQ+GE+SE+ELKQYDGSDP KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQLGEISEEELKQYDGSDPNKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVADGDATPA--- 180
           RALAKMSFE+KDLTGDISGLGPFEL+ALQDWEYKFMSKY KVGT++KPVA+ DA  A   
Sbjct: 121 RALAKMSFEDKDLTGDISGLGPFELDALQDWEYKFMSKYSKVGTLQKPVAEADANAAPAA 180

Query: 181 AGEPAESSTAEVSSSSKPAEDSPSESHADRAAEETPAAAKNVE 220
           AGEPAES+TAEVS   KPAEDS SES A +A E+TPAA  NVE
Sbjct: 181 AGEPAESTTAEVS---KPAEDSTSESRAVKA-EDTPAA--NVE 217

[Close]

BLAST of Cp4.1LG15g03220 vs. TAIR 10
Match: AT3G48890.1 (membrane-associated progesterone binding protein 3 )

HSP 1 Score: 257.3 bits (656), Expect = 1.1e-68
Identity = 140/221 (63.35%), Postives = 164/221 (74.21%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFF-GPSDYGTHTRDLGEQLQ 60
           M  Q+WETLKE I AYTGLSP+ FFTVLAL  A Y ++S FF  P  +   + ++  Q +
Sbjct: 1   MVQQIWETLKETITAYTGLSPAAFFTVLALAFAVYQVVSGFFVSPEVHRPRSLEVQPQSE 60

Query: 61  PLPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120
           PLPPPVQ+GE++E+ELK YDGSD +KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA
Sbjct: 61  PLPPPVQLGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120

Query: 121 SRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVADGDATPAAG 180
           SRALAKMSFE++DLTGDISGLG FELEALQDWEYKFMSKYVKVGTI+K   +G     + 
Sbjct: 121 SRALAKMSFEDQDLTGDISGLGAFELEALQDWEYKFMSKYVKVGTIQKKDGEG---KESS 180

Query: 181 EPAESSTAEV--------SSSSKPAEDSPSESHADRAAEET 213
           EP+E+ TA            +S    D  S S  ++ AE T
Sbjct: 181 EPSEAKTASAEGLSTNTGEEASAITHDETSRSTGEKIAETT 218

[Close]

BLAST of Cp4.1LG15g03220 vs. TAIR 10
Match: AT5G52240.2 (membrane steroid binding protein 1 )

HSP 1 Score: 251.1 bits (640), Expect = 8.0e-67
Identity = 126/171 (73.68%), Postives = 141/171 (82.46%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGP--SDYGTHTRDLG--- 60
           MAL++W+TLKEAI AYTGLSP  FFT LAL  A Y +IS +F     D   H R      
Sbjct: 1   MALELWQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFDDVNRHQRARSLAQ 60

Query: 61  EQLQPLPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFA 120
           E+  P+P PVQVGE++E+ELKQYDGSDP+KPLLMAIK QIYDV+QSRMFYGPGGPYALFA
Sbjct: 61  EEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPYALFA 120

Query: 121 GKDASRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIK 167
           GKDASRALAKMSFEEKDLT D+SGLGPFEL+ALQDWEYKFMSKY KVGT+K
Sbjct: 121 GKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVGTVK 171

[Close]

BLAST of Cp4.1LG15g03220 vs. TAIR 10
Match: AT5G52240.1 (membrane steroid binding protein 1 )

HSP 1 Score: 248.4 bits (633), Expect = 5.2e-66
Identity = 134/215 (62.33%), Postives = 162/215 (75.35%), Query Frame = 0


Query: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGP--SDYGTHTRDLG--- 60
           MAL++W+TLKEAI AYTGLSP  FFT LAL  A Y +IS +F     D   H R      
Sbjct: 1   MALELWQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFDDVNRHQRARSLAQ 60

Query: 61  EQLQPLPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFA 120
           E+  P+P PVQVGE++E+ELKQYDGSDP+KPLLMAIK QIYDV+QSRMFYGPGGPYALFA
Sbjct: 61  EEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPYALFA 120

Query: 121 GKDASRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVADGDAT 180
           GKDASRALAKMSFEEKDLT D+SGLGPFEL+ALQDWEYKFMSKY KVGT+K   ++ + T
Sbjct: 121 GKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVGTVKVAGSEPE-T 180

Query: 181 PAAGEPAESSTAEVSSSSKP-------AEDSPSES 204
            +  EP E+   +   ++ P       ++D+P+E+
Sbjct: 181 ASVSEPTENVEQDAHVTTTPGKTVVDKSDDAPAET 214

[Close]

BLAST of Cp4.1LG15g03220 vs. TAIR 10
Match: AT2G24940.1 (membrane-associated progesterone binding protein 2 )

HSP 1 Score: 108.6 bits (270), Expect = 6.4e-24
Identity = 48/97 (49.48%), Postives = 69/97 (71.13%), Query Frame = 0


Query: 69  EVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSF 128
           E + ++L QY+G+D  KP+ +AIKG+++DV+  + FYG GG Y++FAGKDASRAL KMS 
Sbjct: 2   EFTAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSK 61

Query: 129 EEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTI 166
            E+D++  + GL   E+  L DWE KF +KY  VG +
Sbjct: 62  NEEDVSPSLEGLTEKEINTLNDWETKFEAKYPVVGRV 98

[Close]

BLAST of Cp4.1LG15g03220 vs. TAIR 10
Match: AT4G14965.1 (membrane-associated progesterone binding protein 4 )

HSP 1 Score: 70.9 bits (172), Expect = 1.5e-12
Identity = 45/145 (31.03%), Postives = 70/145 (48.28%), Query Frame = 0


Query: 71  SEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSFEE 130
           S +EL  Y+G+D   P+L+ I G ++DV++ +  YG GG Y  FAG+DASRA    +F  
Sbjct: 43  SAEELALYNGTDETLPILLGILGSVFDVTKGKFHYGSGGGYNHFAGRDASRAFVSGNFTG 102

Query: 131 KDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVADGDATPA----AGEPAESST 190
             LT  + GL   E++++ DW   +   Y  VG +     D    P       E   S  
Sbjct: 103 DGLTDSLQGLSSSEVKSIVDWRGFYSRTYTPVGKLVGRYYDSQGNPTKHLKGAEAKASRG 162

Query: 191 AEVSSSSKPAEDSPSESHADRAAEE 212
           A++    K  E   S  ++  + +E
Sbjct: 163 AQLMEKQKTEEAKQSNCNSRWSQDE 187

The following BLAST results are available for this feature:

BLAST of Cp4.1LG15g03220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2Z4	1.6e-67	63.35	Membrane steroid-binding protein 2 OS=Arabidopsis thaliana OX=3702 GN=MSBP2 PE=1...	[more]
Q9FVZ7	6.0e-67	63.43	Membrane steroid-binding protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=M...	[more]
Q9XFM6	7.3e-65	62.33	Membrane steroid-binding protein 1 OS=Arabidopsis thaliana OX=3702 GN=MSBP1 PE=1...	[more]
Q9FVZ9	9.6e-49	62.29	Membrane steroid-binding protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=M...	[more]
Q9SK39	9.0e-23	49.48	Probable steroid-binding protein 3 OS=Arabidopsis thaliana OX=3702 GN=MP3 PE=1 S...	[more]

BLAST of Cp4.1LG15g03220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023554188.1	7.35e-150	100.00	membrane steroid-binding protein 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_022952639.1	3.48e-146	98.19	membrane steroid-binding protein 2-like [Cucurbita moschata] >XP_022952640.1 mem...	[more]
XP_022971922.1	1.32e-145	97.73	membrane steroid-binding protein 2-like [Cucurbita maxima]	[more]
KAG6571809.1	1.42e-145	97.74	Membrane steroid-binding protein 2, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_038888418.1	1.40e-125	89.35	membrane steroid-binding protein 2-like [Benincasa hispida]	[more]

BLAST of Cp4.1LG15g03220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GMC2	1.68e-146	98.19	membrane steroid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1I394	6.38e-146	97.73	membrane steroid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111470...	[more]
A0A6J1GZX3	1.44e-121	87.16	membrane steroid-binding protein 1-like isoform X1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1K773	8.29e-121	86.04	membrane steroid-binding protein 1-like isoform X1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1E0F8	4.37e-116	82.06	membrane steroid-binding protein 2-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]

BLAST of Cp4.1LG15g03220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48890.1	1.1e-68	63.35	membrane-associated progesterone binding protein 3	[more]
AT5G52240.2	8.0e-67	73.68	membrane steroid binding protein 1	[more]
AT5G52240.1	5.2e-66	62.33	membrane steroid binding protein 1	[more]
AT2G24940.1	6.4e-24	49.48	membrane-associated progesterone binding protein 2	[more]
AT4G14965.1	1.5e-12	31.03	membrane-associated progesterone binding protein 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 70..166 e-value: 3.9E-24 score: 96.2
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 72..165 e-value: 1.2E-14 score: 54.1
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 68..166 e-value: 3.4E-37 score: 128.7
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 61..166
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 184..198
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 199..213
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 169..220
None	No IPR available	PANTHER	PTHR10281	MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED	coord: 1..206
None	No IPR available	PANTHER	PTHR10281:SF90	STEROID-BINDING PROTEIN 3-RELATED	coord: 1..206

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG15g03220.1	Cp4.1LG15g03220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0019904	protein domain specific binding