Cp4.1LG15g02320 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG15g02320
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionserine/arginine-rich splicing factor SC35
LocationCp4.1LG15: 2094951 .. 2099367 (+)
RNA-Seq ExpressionCp4.1LG15g02320
SyntenyCp4.1LG15g02320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATCCATGTACGGAAGGTCAATTAGGGAAGAAGGATTTTACCGATCTGCCCCTCTCTCTGTATAAATATTCTATCTGGGCTCGTCAGGAAAGTTAGTTTCAGGGACCTTTCTTCTCCGTCGTCTCTAACCCAAACCCTAATCTTCATCTTCCAACCAACATGTCGCACTTCGGAAGGTCCGGTCCTCCAGATATTGCAGACACCTATTCGCTTCTCGTGCTCAACATTACCTTCCGTATGATTCCATATATCTCTCTTTTTCTTTCTCAAATTTCCTTGTTTCTTTTGTGTTTTTCTGTTTTGTATCGTGTTCAGTCNTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGTTTTTAGGAACCACTGCTGATGATCTCTACCCGCTGTTCCACAAGTATGGTAAGGTTGTCGATATCTTCATCCCCAGAGACCGAAGGTATGATTCCCTGCTTTGTTGCGTTTGTTTTCTTATGAAATTTGTTCGATTTCTGTAAGAGATGACGATGAATGTTTTTTTTTTTAATAGCTGGTAAGAGTTTTGTGTTTGTTAGGTTAAACATTTTGAGATTAGGATTGAATTGATTTCCTTCCATTGATATTCATCTGGTTCTTATGAAATTAGTTTGCACGTGAGGGTGAAATGATATTCTTCATTCAGCTTAAATTATGCTTGGGCTCAGTTGGGAACTTTATTTATTTTTCTGTTTGTTCATCTTTTGTTAGCCATCAATTATTCTCGTTTTGATTATTTTGTAATTGTCTCTTTTATTGTATCGTGGTTCAGAACTGGTGATTCTAGAGGTTTTGCATTTGTGCGTTACAAGTATGCAGATGAGGCTCAGAAAGCAGTGGAAAGGCTAGATGGTACCCATGCCTCTTTGTTTGTTTCCTGTTGTTTGATTTTGTCCTTTTCCTCTTCAAGTGGAACTAATTTGTAGATACAAGTTACAAGGAAGATGTTTTATATTTTTGTTGGATTGAAGGCTGATAATCTTATGGTCGTTATTCAAATCAGTTTGGGCGGGCCCTATAAGATCAATATTTTCTGAATTAACTCCCTCACTCCTTTGTTAGTTCTGGTTTTGAATTACGATCATGCCTCTACTTACTTCCATTTGATTATTTCTATGCAGTTTGTTTGGGGAGCTAATCTGCTGCATATAAATTTGAAGCTATATATATAATTATTTATTTAATTTTTTAAATTTTGTCATGTTCCAGGTATTGTGGTTGATGGACGAGAGATCACCGTTCAGTTTGCAAAATATGGTCCAAATGCAGAAAGGATGTGAGTAATGAAGTAGTGGTTTTCCTCGATGAATGCTTACGTTTGGGCCTAACTTCTCACCTCTTATATATTTGTTTTGCTAATGGGTGTTGTATTTATGTCAAAATATGCTTTTAGTCACAAAGGAAAGGTCAGTGAATCATTTCCAAAGTCGAGATACAGGTCTAGAAGTCGCAGTCCACGGAGACGGTAATTTTCTCTTCCACATCCCTATGAATTTTTTATATTGGGATTAAATATTTGTTGTCCTTATCCTTGAAGAATATTGTCTGGTCATTAGAGTTCCTTGGTGGAATTATTATTTCTTCAGCTTTAAGATGTTCAAGGGTGAAAGCTTCGGTTGAAATAGGATATTCGAGGAATATGATGCAGCCACATTTTTGAACCCTAAGATATTGTGTTAACTGTTTGTGCCTAGGATTACTTTAGCAAGCTCTTGTTTTCTGCTATCTCAGATATGAGGTTCGAAGATGCTGTTTTGTCCAAGTTTTTCATTCTTTTTTAGGTTAGATATGGTATGCTGTTTTAACATGAGATACTATAATTATGGAAACGTAAAATGTTGGAAGATCTAAAAGGAGACTGTAGCAGTGTATTCTATATATGGCTAGGACTAGATAGTGGTGCTCAAACCTTTTCTTTCATGGAGTTCAAATTTTGACCATTTGCATGTAAAGTAACTACTTGGAATTTCTTACTTTCTTTCTATTAAGATCCATTACTTTATGAGCTTTTGTAGTTTTATCTTCATTTTTTTAAATTCAAGTTACTAATTGAAATTTATTACTTTCAGACATCGGGATGATCACAGAGATAGGGATTATAGGAGAAGAAGTCGAAGCAGAAGTAGAAGCAGAAGCTTGGACAGGGTTGATCATGATAGGCGTGGAAGAGAAAGGGAGAGGGAAAGAGACTATCGTAGGCGAAGCCGGAGCATCAGTCCTAGGCGGCCTAGTCCTGATTATTCCAAGGGGAGGGGAAGAGGACGCTACGTTGATGAGCGTAGAAGCCGCAGCCGCAGCCGTAGCCGTAGCCATAGCCAAAGTCGTGGCCGTGGTCGTGGTCGTTCTGTGGATAGGTTGTTAATTTCTTACAATTTTTATCCAGTTGGAAGAAAGTTGGGAATTGTATTTCTAATCATCTTTATCCATTTGGGAAAAAGTGCGGAAAATCTTATTTCTAATCAGTCATTGTTCTACATATTACAGTGCTGCTTCTCCTGGTCGACAAAGCCCTAGTCCAAGGAGTGCATCTCCTCAGGGGAGTCCATCTCCCAGGGGTGAAAGTCCTGAAAGAAATGGCGATGGTGGAAGGTCTCCAACTTCCAGGAGTGTTTCACCCCGAGGTCAACCTGCTGATTCAAGAAGCCCATCTCAACCAAATTCAGATGTTGATGTGAGTTGCATTAGGAACTATTTTAAATGATTAGTCTAGACTTGGTGTTAGTAGTTAAATGTTCACTTGTCTTAATTTTGACAGAAGTGAATACTGATGGATGCAAGGAGAACTGTTACATTTCCGAGCAGTGCAGGAATGGATGCTTTGAGGACTCGTTGATTAAAAATGTTATCAGACTATAAGTTCTAGTTTTGGTAGGACTTTCCTATGGTGTTCAATTATCATATATTGAGCTTAATGTGCCTATCTACTATCTTGTATCAGTTTGATACTTTTTGGCTCTGCTGTCTGTATCCTTATATCCAGTCATAGGATATAGCTTGGGACATGTTCTTCCTCCTTTATATTTCTCCTCTCCACCCTGTAGTACTAGTAATATTTTACCTTGAGCAATTGTGGTGTCTTTTTCTTTTCCCCATTTTCCTTTTTTTTAATAAATATTTTAGCTATCCTGATTCATCATTGAATCCTCTATTGGATTTTATAGTTATGTTCGGGGTAAGAGTGACCGGCGCCTGATTTGGCCGATGTTGATTTTGTGGCTCTTGATGCTGTTTCTGTGTGATAGATGGGTGATCGTGACTTTGATGCTGGTTGCAGCAGTTTGCTGATTTATCAGTATGATTGTCTATGCAGTATGATGTTGCTGTGGACAGTGTCTTAACTAATATTCACTGGATGAAAAAATTATTGGTAAGGTTTTGGCCACATTTGAATGTTTTTAAAAACTTGATAATGCTTTAGGATTTGATGTTTGGCCTGATTATTCATTCTGGTCTATCCAAGTTTTGAAAGTTGCACCTCACCTGCCAAGGAGATCTAGAGGCTTTGATATATAGGGTTGACCCTCCTGTGTTGATCGAATTTTACTCATAGCTGATGATGCATTTGAGATCGGTATTGATTTTAATGGCGAAGGCTGGATTAAGAAAACTTAGCTGCAGGTTTGATCTTTGCGTCCGAACATGATTGGCCACGGTAACATAAGTTTATAGTTTTGTGTATTAGTTTATAGTTGTGTATTATCCGAACATGACAGGTTAGTATCCCAAACATTTAAATCAATCTCTTGCTTGCTGCTTGATGTGGTGACTGACTTGTTGTAGCTTTTGGATACACAATTGTTGCGGTGGTGTTTGATAGCAGATATCTTTATTGATGTACGATAGTCATCGGTTCGATTGATTGATTGATTCTGAAGTGCTAACGCTGTAGCTGGCTGTTGCTGCCCTTGTGGGGGTGAGGCTGGCAATGATGTTTGCAGTTTGCTTTGACAATGGATGCATTGATCTTTGCTTGCCTCGAAATAAAGTTCTGCACGTATGTTCTCGCTCACTTCATGTCTGTAAGAAAATTTGCTTGATAAAAATTCTGACATTGATTTGCAACATAAATTAAGTAGTTTGGTGAACACGTACGACTACGTGCTTGGTGAACACGATTACTATATTGCTTTTAGCTGCAACTAGGTTTTGGATTTGGAAATACTGCCTATCTGATCTTTTATCTATTGCTCGAAAGAGAGATGCTTTAACTGGCAGGAAGCTTGTTAGATTGCTTGCATCCTTTTTATGGTTTATTCCATTACAGAGTCTGCTTTTAGATGCTTGCAGATTTTTTACATCAACAATTTTGTTTGCTCTAATTTTTTTACATCCAAACAAAATGAGGAACATTGACAAAGTATTTCCAATGTTATCAGGTAAGTTGTGTTGTGTTTGATCATTGA

mRNA sequence

ATGGAATCCATGTACGGAAGGTCAATTAGGGAAGAAGGATTTTACCGATCTGCCCCTCTCTCTGTATAAATATTCTATCTGGGCTCGTCAGGAAAGTTAGTTTCAGGGACCTTTCTTCTCCGTCGTCTCTAACCCAAACCCTAATCTTCATCTTCCAACCAACATGTCGCACTTCGGAAGGTCCGGTCCTCCAGATATTGCAGACACCTATTCGCTTCTCGTGCTCAACATTACCTTCCGAACCACTGCTGATGATCTCTACCCGCTGTTCCACAAGTATGGTAAGGTTGTCGATATCTTCATCCCCAGAGACCGAAGAACTGGTGATTCTAGAGGTTTTGCATTTGTGCGTTACAAGTATGCAGATGAGGCTCAGAAAGCAGTGGAAAGGCTAGATGGTATTGTGGTTGATGGACGAGAGATCACCGTTCAGTTTGCAAAATATGGTCCAAATGCAGAAAGGATTCACAAAGGAAAGGTCAGTGAATCATTTCCAAAGTCGAGATACAGGTCTAGAAGTCGCAGTCCACGGAGACGATATGAGGTTCGAAGATGCTGTTTTGTCCAAGTTTTTCATTCTTTTTTAGACATCGGGATGATCACAGAGATAGGGATTATAGGAGAAGAAGTCGAAGCAGAAGTAGAAGCAGAAGCTTGGACAGGTGCTGCTTCTCCTGGTCGACAAAGCCCTAGTCCAAGGAGTGCATCTCCTCAGGGGAGTCCATCTCCCAGGGGTGAAAGTCCTGAAAGAAATGGCGATGGTGGAAGGTCTCCAACTTCCAGGAGTGTTTCACCCCGAGGTCAACCTGCTGATTCAAGAAGCCCATCTCAACCAAATTCAGATGTTGATTATGATGTTGCTGTGGACAGTGTCTTAACTAATATTCACTGGATGAAAAAATTATTGCTGGCTGTTGCTGCCCTTGTGGGGGTGAGGCTGGCAATGATGTTTGCAGTTTGCTTTGACAATGGATGCATTGATCTTTGCTTGCCTCGAAATAAAGTTCTGCACGTAAGTTGTGTTGTGTTTGATCATTGA

Coding sequence (CDS)

ATGTCGCACTTCGGAAGGTCCGGTCCTCCAGATATTGCAGACACCTATTCGCTTCTCGTGCTCAACATTACCTTCCGAACCACTGCTGATGATCTCTACCCGCTGTTCCACAAGTATGGTAAGGTTGTCGATATCTTCATCCCCAGAGACCGAAGAACTGGTGATTCTAGAGGTTTTGCATTTGTGCGTTACAAGTATGCAGATGAGGCTCAGAAAGCAGTGGAAAGGCTAGATGGTATTGTGGTTGATGGACGAGAGATCACCGTTCAGTTTGCAAAATATGGTCCAAATGCAGAAAGGATTCACAAAGGAAAGGTCAGTGAATCATTTCCAAAGTCGAGATACAGGTCTAGAAGTCGCAGTCCACGGAGACGATATGAGGTTCGAAGATGCTGTTTTGTCCAAGTTTTTCATTCTTTTTTAGACATCGGGATGATCACAGAGATAGGGATTATAGGAGAAGAAGTCGAAGCAGAAGTAGAAGCAGAAGCTTGGACAGGTGCTGCTTCTCCTGGTCGACAAAGCCCTAGTCCAAGGAGTGCATCTCCTCAGGGGAGTCCATCTCCCAGGGGTGAAAGTCCTGAAAGAAATGGCGATGGTGGAAGGTCTCCAACTTCCAGGAGTGTTTCACCCCGAGGTCAACCTGCTGATTCAAGAAGCCCATCTCAACCAAATTCAGATGTTGATTATGATGTTGCTGTGGACAGTGTCTTAACTAATATTCACTGGATGAAAAAATTATTGCTGGCTGTTGCTGCCCTTGTGGGGGTGAGGCTGGCAATGATGTTTGCAGTTTGCTTTGACAATGGATGCATTGATCTTTGCTTGCCTCGAAATAAAGTTCTGCACGTAAGTTGTGTTGTGTTTGATCATTGA

Protein sequence

MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFAFVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSRSPRRRYEVRRCCFVQVFHSFLDIGMITEIGIIGEEVEAEVEAEAWTGAASPGRQSPSPRSASPQGSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNSDVDYDVAVDSVLTNIHWMKKLLLAVAALVGVRLAMMFAVCFDNGCIDLCLPRNKVLHVSCVVFDH
Homology
BLAST of Cp4.1LG15g02320 vs. ExPASy Swiss-Prot
Match: Q9FMG4 (Serine/arginine-rich splicing factor SC35 OS=Arabidopsis thaliana OX=3702 GN=SC35 PE=1 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 8.5e-53
Identity = 140/272 (51.47%), Postives = 159/272 (58.46%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDI+DTYSLLVLNITFRTTADDLYPLF KYGKVVD+FIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDISDTYSLLVLNITFRTTADDLYPLFAKYGKVVDVFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKY DEA KAVERLDG VVDGREITVQFAKYGPNAE+I KG+V E  PKSR RSRSR
Sbjct: 61  FVRYKYKDEAHKAVERLDGRVVDGREITVQFAKYGPNAEKISKGRVVEPPPKSR-RSRSR 120

Query: 121 SPRRRYEVRRCCFV------------------------------------QVFHSFLD-- 180
           SPRR    RR                                        +  H   D  
Sbjct: 121 SPRRSRSPRRSRSPPRRRSPRRSRSPRRRSRDDYREKDYRKRSRSRSYDRRERHEEKDRD 180

Query: 181 --------IGMITEIGIIGEEVEAEVEAEAWTGAASPGRQSPSPRSASPQGSPSPRGESP 227
                        E   +    + E  + + + +ASP R+  SPRS+SPQ +   R  SP
Sbjct: 181 HRRRTRSRSASPDEKRRVRGRYDNESRSHSRSLSASPARR--SPRSSSPQKTSPAREVSP 240

BLAST of Cp4.1LG15g02320 vs. ExPASy Swiss-Prot
Match: P30352 (Serine/arginine-rich splicing factor 2 OS=Gallus gallus OX=9031 GN=SRSF2 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 7.5e-25
Identity = 61/128 (47.66%), Postives = 84/128 (65.62%), Query Frame = 0

Query: 4   FGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFAFVR 63
           +GR  PPD+    SL V N+T+RT+ D L  +F KYG+V D++IPRDR T +SRGFAFVR
Sbjct: 3   YGRP-PPDVEGMTSLKVDNLTYRTSPDTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVR 62

Query: 64  YKYADEAQKAVERLDGIVVDGREITVQFAKYG--PNAERIHKGKVSESFPKSRYRSRSRS 123
           +    +A+ A++ +DG V+DGRE+ VQ A+YG  P++    +G     +  S Y  RSRS
Sbjct: 63  FHDKRDAEDAMDAMDGAVLDGRELRVQMARYGRPPDSHHSRRGPPPRRYGSSGYGRRSRS 122

Query: 124 PRRRYEVR 130
           PRRR   R
Sbjct: 123 PRRRRRSR 129

BLAST of Cp4.1LG15g02320 vs. ExPASy Swiss-Prot
Match: Q3MHR5 (Serine/arginine-rich splicing factor 2 OS=Bos taurus OX=9913 GN=SRSF2 PE=2 SV=3)

HSP 1 Score: 99.8 bits (247), Expect = 5.6e-20
Identity = 81/225 (36.00%), Postives = 115/225 (51.11%), Query Frame = 0

Query: 4   FGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFAFVR 63
           +GR  PPD+    SL V N+T+RT+ D L  +F KYG+V D++IPRDR T +SRGFAFVR
Sbjct: 3   YGRP-PPDVEGMTSLKVDNLTYRTSPDTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVR 62

Query: 64  YKYADEAQKAVERLDGIVVDGREITVQFAKYG--PNAERIHKGKVSESFPKSRYRSRSRS 123
           +    +A+ A++ +DG V+DGRE+ VQ A+YG  P++    +G     +    Y  RSRS
Sbjct: 63  FHDKRDAEDAMDAMDGAVLDGRELRVQMARYGRPPDSHHSRRGPPPRRYGGGGYGRRSRS 122

Query: 124 PRRRYEVRRCCFVQVFHSFLDIGMITEIGIIGEEVEAEVEAEAWTGAASPGRQSPSPRSA 183
           PRRR   R     +           +          ++  +   + + S  +   + RS 
Sbjct: 123 PRRRRRSRSRSRSR-----------SRSRSRSRYSRSKSRSRTRSRSRSTSKSRSARRSK 182

Query: 184 SPQGSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNS 227
           S   S S R  S  R+    RSP    VS R   + SRS S P S
Sbjct: 183 SKSSSVS-RSRSRSRSRSRSRSPP--PVSKRESKSRSRSKSPPKS 212

BLAST of Cp4.1LG15g02320 vs. ExPASy Swiss-Prot
Match: Q01130 (Serine/arginine-rich splicing factor 2 OS=Homo sapiens OX=9606 GN=SRSF2 PE=1 SV=4)

HSP 1 Score: 99.8 bits (247), Expect = 5.6e-20
Identity = 81/225 (36.00%), Postives = 115/225 (51.11%), Query Frame = 0

Query: 4   FGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFAFVR 63
           +GR  PPD+    SL V N+T+RT+ D L  +F KYG+V D++IPRDR T +SRGFAFVR
Sbjct: 3   YGRP-PPDVEGMTSLKVDNLTYRTSPDTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVR 62

Query: 64  YKYADEAQKAVERLDGIVVDGREITVQFAKYG--PNAERIHKGKVSESFPKSRYRSRSRS 123
           +    +A+ A++ +DG V+DGRE+ VQ A+YG  P++    +G     +    Y  RSRS
Sbjct: 63  FHDKRDAEDAMDAMDGAVLDGRELRVQMARYGRPPDSHHSRRGPPPRRYGGGGYGRRSRS 122

Query: 124 PRRRYEVRRCCFVQVFHSFLDIGMITEIGIIGEEVEAEVEAEAWTGAASPGRQSPSPRSA 183
           PRRR   R     +           +          ++  +   + + S  +   + RS 
Sbjct: 123 PRRRRRSRSRSRSR-----------SRSRSRSRYSRSKSRSRTRSRSRSTSKSRSARRSK 182

Query: 184 SPQGSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNS 227
           S   S S R  S  R+    RSP    VS R   + SRS S P S
Sbjct: 183 SKSSSVS-RSRSRSRSRSRSRSPP--PVSKRESKSRSRSKSPPKS 212

BLAST of Cp4.1LG15g02320 vs. ExPASy Swiss-Prot
Match: Q62093 (Serine/arginine-rich splicing factor 2 OS=Mus musculus OX=10090 GN=Srsf2 PE=1 SV=4)

HSP 1 Score: 99.8 bits (247), Expect = 5.6e-20
Identity = 81/225 (36.00%), Postives = 115/225 (51.11%), Query Frame = 0

Query: 4   FGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFAFVR 63
           +GR  PPD+    SL V N+T+RT+ D L  +F KYG+V D++IPRDR T +SRGFAFVR
Sbjct: 3   YGRP-PPDVEGMTSLKVDNLTYRTSPDTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVR 62

Query: 64  YKYADEAQKAVERLDGIVVDGREITVQFAKYG--PNAERIHKGKVSESFPKSRYRSRSRS 123
           +    +A+ A++ +DG V+DGRE+ VQ A+YG  P++    +G     +    Y  RSRS
Sbjct: 63  FHDKRDAEDAMDAMDGAVLDGRELRVQMARYGRPPDSHHSRRGPPPRRYGGGGYGRRSRS 122

Query: 124 PRRRYEVRRCCFVQVFHSFLDIGMITEIGIIGEEVEAEVEAEAWTGAASPGRQSPSPRSA 183
           PRRR   R     +           +          ++  +   + + S  +   + RS 
Sbjct: 123 PRRRRRSRSRSRSR-----------SRSRSRSRYSRSKSRSRTRSRSRSTSKSRSARRSK 182

Query: 184 SPQGSPSPRGESPERNGDGGRSPTSRSVSPRGQPADSRSPSQPNS 227
           S   S S R  S  R+    RSP    VS R   + SRS S P S
Sbjct: 183 SKSSSVS-RSRSRSRSRSRSRSPP--PVSKRESKSRSRSKSPPKS 212

BLAST of Cp4.1LG15g02320 vs. NCBI nr
Match: XP_023554224.1 (serine/arginine-rich splicing factor SC35-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 330 bits (847), Expect = 2.94e-110
Identity = 192/282 (68.09%), Postives = 199/282 (70.57%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR
Sbjct: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120

Query: 121 SPRRRYEV---------------------------------------RRCCFVQVFHSFL 180
           SPRRR+                                         RR   +       
Sbjct: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRVDHDRRGRERERERDYRRRSRSISPRRPSP 180

Query: 181 DIGMITEIGIIGEEVEAEVEAEAWT--------------GAASPGRQSPSPRSASPQGSP 229
           D       G   +E  +   + + +               AASPGRQSPSPRSASPQGSP
Sbjct: 181 DYSKGRGRGRYVDERRSRSRSRSRSHSQSRGRGRGRSVDSAASPGRQSPSPRSASPQGSP 240

BLAST of Cp4.1LG15g02320 vs. NCBI nr
Match: XP_023554223.1 (serine/arginine-rich splicing factor SC35-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 330 bits (847), Expect = 3.04e-110
Identity = 192/282 (68.09%), Postives = 199/282 (70.57%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR
Sbjct: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120

Query: 121 SPRRRYEV---------------------------------------RRCCFVQVFHSFL 180
           SPRRR+                                         RR   +       
Sbjct: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRVDHDRRGRERERERDYRRRSRSISPRRPSP 180

Query: 181 DIGMITEIGIIGEEVEAEVEAEAWT--------------GAASPGRQSPSPRSASPQGSP 229
           D       G   +E  +   + + +               AASPGRQSPSPRSASPQGSP
Sbjct: 181 DYSKGRGRGRYVDERRSRSRSRSRSHSQSRGRGRGRSVDSAASPGRQSPSPRSASPQGSP 240

BLAST of Cp4.1LG15g02320 vs. NCBI nr
Match: KAG7011405.1 (Serine/arginine-rich splicing factor SC35 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 326 bits (835), Expect = 3.98e-108
Identity = 191/288 (66.32%), Postives = 200/288 (69.44%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR
Sbjct: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120

Query: 121 SPRRRYEV---------------------------------------RRCCFVQVFHSFL 180
           SPRRR+                                         RR   +       
Sbjct: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180

Query: 181 DIGMITEIGIIGEEVEAEVEAEAWT-----------------GAASPGRQSPSPRSASPQ 232
           D       G   +E  +   + + +                  +ASPGRQSPSPRSASPQ
Sbjct: 181 DYSKGRGRGRYIDERRSRSRSRSRSHSHSHSQSRGRGRGRSVDSASPGRQSPSPRSASPQ 240

BLAST of Cp4.1LG15g02320 vs. NCBI nr
Match: XP_022963472.1 (serine/arginine-rich splicing factor SC35-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 322 bits (826), Expect = 5.20e-107
Identity = 189/285 (66.32%), Postives = 197/285 (69.12%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR
Sbjct: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120

Query: 121 SPRRRYEV---------------------------------------RRCCFVQVFHSFL 180
           SPRRR+                                         RR   +       
Sbjct: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180

Query: 181 DIGMITEIGIIGEEVEAEVEAEAWT-----------------GAASPGRQSPSPRSASPQ 229
           D       G   +E  +   + + +                  +ASPGRQSPSPRSASP 
Sbjct: 181 DYSKGRGRGRYIDERRSRSRSRSRSRSHSHSQSRGRGRGRSVDSASPGRQSPSPRSASPH 240

BLAST of Cp4.1LG15g02320 vs. NCBI nr
Match: XP_022963473.1 (serine/arginine-rich splicing factor SC35-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 322 bits (824), Expect = 1.05e-106
Identity = 189/286 (66.08%), Postives = 197/286 (68.88%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR
Sbjct: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120

Query: 121 SPRRRYEV---------------------------------------RRCCFVQVFHSFL 180
           SPRRR+                                         RR   +       
Sbjct: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180

Query: 181 DIGMITEIGIIGEEVEAEVEAEAWTGA------------------ASPGRQSPSPRSASP 229
           D       G   +E  +   + + + +                  ASPGRQSPSPRSASP
Sbjct: 181 DYSKGRGRGRYIDERRSRSRSRSRSRSHSHSQSRGRGRGRSVDRFASPGRQSPSPRSASP 240

BLAST of Cp4.1LG15g02320 vs. ExPASy TrEMBL
Match: A0A6J1HFC7 (serine/arginine-rich splicing factor SC35-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111463797 PE=4 SV=1)

HSP 1 Score: 322 bits (826), Expect = 2.52e-107
Identity = 189/285 (66.32%), Postives = 197/285 (69.12%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR
Sbjct: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120

Query: 121 SPRRRYEV---------------------------------------RRCCFVQVFHSFL 180
           SPRRR+                                         RR   +       
Sbjct: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180

Query: 181 DIGMITEIGIIGEEVEAEVEAEAWT-----------------GAASPGRQSPSPRSASPQ 229
           D       G   +E  +   + + +                  +ASPGRQSPSPRSASP 
Sbjct: 181 DYSKGRGRGRYIDERRSRSRSRSRSRSHSHSQSRGRGRGRSVDSASPGRQSPSPRSASPH 240

BLAST of Cp4.1LG15g02320 vs. ExPASy TrEMBL
Match: A0A6J1HHW9 (serine/arginine-rich splicing factor SC35-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111463797 PE=4 SV=1)

HSP 1 Score: 322 bits (824), Expect = 5.06e-107
Identity = 189/286 (66.08%), Postives = 197/286 (68.88%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR
Sbjct: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120

Query: 121 SPRRRYEV---------------------------------------RRCCFVQVFHSFL 180
           SPRRR+                                         RR   +       
Sbjct: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180

Query: 181 DIGMITEIGIIGEEVEAEVEAEAWTGA------------------ASPGRQSPSPRSASP 229
           D       G   +E  +   + + + +                  ASPGRQSPSPRSASP
Sbjct: 181 DYSKGRGRGRYIDERRSRSRSRSRSRSHSHSQSRGRGRGRSVDRFASPGRQSPSPRSASP 240

BLAST of Cp4.1LG15g02320 vs. ExPASy TrEMBL
Match: A0A6J1HG77 (serine/arginine-rich splicing factor SC35-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111463797 PE=4 SV=1)

HSP 1 Score: 322 bits (824), Expect = 5.24e-107
Identity = 189/286 (66.08%), Postives = 197/286 (68.88%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR
Sbjct: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120

Query: 121 SPRRRYEV---------------------------------------RRCCFVQVFHSFL 180
           SPRRR+                                         RR   +       
Sbjct: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180

Query: 181 DIGMITEIGIIGEEVEAEVEAEAWTGA------------------ASPGRQSPSPRSASP 229
           D       G   +E  +   + + + +                  ASPGRQSPSPRSASP
Sbjct: 181 DYSKGRGRGRYIDERRSRSRSRSRSRSHSHSQSRGRGRGRSVDRFASPGRQSPSPRSASP 240

BLAST of Cp4.1LG15g02320 vs. ExPASy TrEMBL
Match: A0A6J1HVK2 (serine/arginine-rich splicing factor SC35-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467060 PE=4 SV=1)

HSP 1 Score: 315 bits (808), Expect = 1.23e-104
Identity = 188/282 (66.67%), Postives = 196/282 (69.50%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSR RSRSR
Sbjct: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRNRSRSR 120

Query: 121 SPRRRYEV---------------------------------------RRCCFVQVFHSFL 180
           SPRRR+                                         RR   +       
Sbjct: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180

Query: 181 DIGMITEIGIIGEEVEAEVEAEAWT-------------GAASPGRQSPSPR-SASPQGSP 229
           D       G   +E  +   + + +              +ASPGRQSPSPR SASPQ SP
Sbjct: 181 DYSKGRGRGRYVDERRSRSRSRSHSYSQTRGRGRGRSVDSASPGRQSPSPRRSASPQRSP 240

BLAST of Cp4.1LG15g02320 vs. ExPASy TrEMBL
Match: A0A6J1HX79 (serine/arginine-rich splicing factor SC35-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111467060 PE=4 SV=1)

HSP 1 Score: 314 bits (805), Expect = 3.50e-104
Identity = 188/283 (66.43%), Postives = 196/283 (69.26%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSR RSRSR
Sbjct: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRNRSRSR 120

Query: 121 SPRRRYEV---------------------------------------RRCCFVQVFHSFL 180
           SPRRR+                                         RR   +       
Sbjct: 121 SPRRRHRDDHRDRDYRRRSRSRSRSRSLDRIDHDRRGRERERERDYRRRSRSISPRRPSP 180

Query: 181 DIGMITEIGIIGEEVEAEVEAEAWTGA--------------ASPGRQSPSPR-SASPQGS 229
           D       G   +E  +   + + + +              ASPGRQSPSPR SASPQ S
Sbjct: 181 DYSKGRGRGRYVDERRSRSRSRSHSYSQTRGRGRGRSVDRFASPGRQSPSPRRSASPQRS 240

BLAST of Cp4.1LG15g02320 vs. TAIR 10
Match: AT5G64200.1 (ortholog of human splicing factor SC35 )

HSP 1 Score: 208.8 bits (530), Expect = 6.0e-54
Identity = 140/272 (51.47%), Postives = 159/272 (58.46%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDI+DTYSLLVLNITFRTTADDLYPLF KYGKVVD+FIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDISDTYSLLVLNITFRTTADDLYPLFAKYGKVVDVFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKY DEA KAVERLDG VVDGREITVQFAKYGPNAE+I KG+V E  PKSR RSRSR
Sbjct: 61  FVRYKYKDEAHKAVERLDGRVVDGREITVQFAKYGPNAEKISKGRVVEPPPKSR-RSRSR 120

Query: 121 SPRRRYEVRRCCFV------------------------------------QVFHSFLD-- 180
           SPRR    RR                                        +  H   D  
Sbjct: 121 SPRRSRSPRRSRSPPRRRSPRRSRSPRRRSRDDYREKDYRKRSRSRSYDRRERHEEKDRD 180

Query: 181 --------IGMITEIGIIGEEVEAEVEAEAWTGAASPGRQSPSPRSASPQGSPSPRGESP 227
                        E   +    + E  + + + +ASP R+  SPRS+SPQ +   R  SP
Sbjct: 181 HRRRTRSRSASPDEKRRVRGRYDNESRSHSRSLSASPARR--SPRSSSPQKTSPAREVSP 240

BLAST of Cp4.1LG15g02320 vs. TAIR 10
Match: AT5G64200.2 (ortholog of human splicing factor SC35 )

HSP 1 Score: 208.8 bits (530), Expect = 6.0e-54
Identity = 140/272 (51.47%), Postives = 159/272 (58.46%), Query Frame = 0

Query: 1   MSHFGRSGPPDIADTYSLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFA 60
           MSHFGRSGPPDI+DTYSLLVLNITFRTTADDLYPLF KYGKVVD+FIPRDRRTGDSRGFA
Sbjct: 1   MSHFGRSGPPDISDTYSLLVLNITFRTTADDLYPLFAKYGKVVDVFIPRDRRTGDSRGFA 60

Query: 61  FVRYKYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSR 120
           FVRYKY DEA KAVERLDG VVDGREITVQFAKYGPNAE+I KG+V E  PKSR RSRSR
Sbjct: 61  FVRYKYKDEAHKAVERLDGRVVDGREITVQFAKYGPNAEKISKGRVVEPPPKSR-RSRSR 120

Query: 121 SPRRRYEVRRCCFV------------------------------------QVFHSFLD-- 180
           SPRR    RR                                        +  H   D  
Sbjct: 121 SPRRSRSPRRSRSPPRRRSPRRSRSPRRRSRDDYREKDYRKRSRSRSYDRRERHEEKDRD 180

Query: 181 --------IGMITEIGIIGEEVEAEVEAEAWTGAASPGRQSPSPRSASPQGSPSPRGESP 227
                        E   +    + E  + + + +ASP R+  SPRS+SPQ +   R  SP
Sbjct: 181 HRRRTRSRSASPDEKRRVRGRYDNESRSHSRSLSASPARR--SPRSSSPQKTSPAREVSP 240

BLAST of Cp4.1LG15g02320 vs. TAIR 10
Match: AT5G18810.1 (SC35-like splicing factor 28 )

HSP 1 Score: 81.6 bits (200), Expect = 1.1e-15
Identity = 47/122 (38.52%), Postives = 71/122 (58.20%), Query Frame = 0

Query: 18  LLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFAFVRYKYADEAQKAVERL 77
           LL+ N+      +DL   F ++G + DI++PR+  TG+ RGF FV+Y+YA++A +A++R+
Sbjct: 49  LLIRNLPLDARPNDLRDSFERFGPLKDIYLPRNYYTGEPRGFGFVKYRYAEDAAEAMKRM 108

Query: 78  DGIVVDGREITVQFAKYG---PNAERIHKG---------KVSESFPKSRYR--SRSRSPR 126
           +  V+ GREI + FA+     P   R   G         + S   P+ RYR  SRSRSP 
Sbjct: 109 NHKVIGGREIAIVFAEENRKTPQEMRTTNGTSGRHGDYKRTSHRSPRRRYRSHSRSRSPP 168

BLAST of Cp4.1LG15g02320 vs. TAIR 10
Match: AT1G55310.1 (SC35-like splicing factor 33 )

HSP 1 Score: 64.3 bits (155), Expect = 1.8e-10
Identity = 72/225 (32.00%), Postives = 101/225 (44.89%), Query Frame = 0

Query: 17  SLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTGDSRGFAFVRYKYADEAQKAVER 76
           SLLV N+      +DL   F ++G V DI++PRD  TGD RGF FV++    +A  A   
Sbjct: 37  SLLVRNLRHDCRQEDLRKSFEQFGPVKDIYLPRDYYTGDPRGFGFVQFMDPADAADAKHH 96

Query: 77  LDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSRSPRRRYEVRRCCFVQV 136
           +DG ++ GRE+TV FA+   N ++  + +  E     R+R R R+P R Y   R      
Sbjct: 97  MDGYLLLGRELTVVFAE--ENRKKPTEMRARER-GGGRFRDRRRTPPRYYSRSR------ 156

Query: 137 FHSFLDIGMITEIGIIGEEVEAEVEAEAWTGAASPGRQSPSPRSASPQGSPSPRGE---- 196
                                +       + + S    SP PR   P+ S SPR E    
Sbjct: 157 ---------------------SPPPRRGRSRSRSGDYYSPPPRRHHPR-SISPREERYDG 216

Query: 197 ------SPERNGDGGRSPT-----SRSVSPRGQPADSRSPSQPNS 227
                 SP  +G  GRS T     SRS+SP  + + SRSP +  S
Sbjct: 217 RRSYSRSPASDGSRGRSLTPVRGKSRSLSPSPRRSISRSPRRSRS 230

BLAST of Cp4.1LG15g02320 vs. TAIR 10
Match: AT1G55310.3 (SC35-like splicing factor 33 )

HSP 1 Score: 60.5 bits (145), Expect = 2.7e-09
Identity = 73/237 (30.80%), Postives = 102/237 (43.04%), Query Frame = 0

Query: 17  SLLVLNITFRTTADDLYPLFHKYGKVVDIFIPRDRRTG------------DSRGFAFVRY 76
           SLLV N+      +DL   F ++G V DI++PRD  TG            D RGF FV++
Sbjct: 37  SLLVRNLRHDCRQEDLRKSFEQFGPVKDIYLPRDYYTGSHLCTDTCKASRDPRGFGFVQF 96

Query: 77  KYADEAQKAVERLDGIVVDGREITVQFAKYGPNAERIHKGKVSESFPKSRYRSRSRSPRR 136
               +A  A   +DG ++ GRE+TV FA+   N ++  + +  E    SR+R R R+P R
Sbjct: 97  MDPADAADAKHHMDGYLLLGRELTVVFAE--ENRKKPTEMRARERGGGSRFRDRRRTPPR 156

Query: 137 RYEVRRCCFVQVFHSFLDIGMITEIGIIGEEVEAEVEAEAWTGAASPGRQSPSPRSASPQ 196
            Y   R                           +       + + S    SP PR   P+
Sbjct: 157 YYSRSR---------------------------SPPPRRGRSRSRSGDYYSPPPRRHHPR 216

Query: 197 GSPSPRGE----------SPERNGDGGRSPT-----SRSVSPRGQPADSRSPSQPNS 227
            S SPR E          SP  +G  GRS T     SRS+SP  + + SRSP +  S
Sbjct: 217 -SISPREERYDGRRSYSRSPASDGSRGRSLTPVRGKSRSLSPSPRRSISRSPRRSRS 243

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMG48.5e-5351.47Serine/arginine-rich splicing factor SC35 OS=Arabidopsis thaliana OX=3702 GN=SC3... [more]
P303527.5e-2547.66Serine/arginine-rich splicing factor 2 OS=Gallus gallus OX=9031 GN=SRSF2 PE=2 SV... [more]
Q3MHR55.6e-2036.00Serine/arginine-rich splicing factor 2 OS=Bos taurus OX=9913 GN=SRSF2 PE=2 SV=3[more]
Q011305.6e-2036.00Serine/arginine-rich splicing factor 2 OS=Homo sapiens OX=9606 GN=SRSF2 PE=1 SV=... [more]
Q620935.6e-2036.00Serine/arginine-rich splicing factor 2 OS=Mus musculus OX=10090 GN=Srsf2 PE=1 SV... [more]
Match NameE-valueIdentityDescription
XP_023554224.12.94e-11068.09serine/arginine-rich splicing factor SC35-like isoform X2 [Cucurbita pepo subsp.... [more]
XP_023554223.13.04e-11068.09serine/arginine-rich splicing factor SC35-like isoform X1 [Cucurbita pepo subsp.... [more]
KAG7011405.13.98e-10866.32Serine/arginine-rich splicing factor SC35 [Cucurbita argyrosperma subsp. argyros... [more]
XP_022963472.15.20e-10766.32serine/arginine-rich splicing factor SC35-like isoform X2 [Cucurbita moschata][more]
XP_022963473.11.05e-10666.08serine/arginine-rich splicing factor SC35-like isoform X3 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1HFC72.52e-10766.32serine/arginine-rich splicing factor SC35-like isoform X2 OS=Cucurbita moschata ... [more]
A0A6J1HHW95.06e-10766.08serine/arginine-rich splicing factor SC35-like isoform X3 OS=Cucurbita moschata ... [more]
A0A6J1HG775.24e-10766.08serine/arginine-rich splicing factor SC35-like isoform X1 OS=Cucurbita moschata ... [more]
A0A6J1HVK21.23e-10466.67serine/arginine-rich splicing factor SC35-like isoform X2 OS=Cucurbita maxima OX... [more]
A0A6J1HX793.50e-10466.43serine/arginine-rich splicing factor SC35-like isoform X3 OS=Cucurbita maxima OX... [more]
Match NameE-valueIdentityDescription
AT5G64200.16.0e-5451.47ortholog of human splicing factor SC35 [more]
AT5G64200.26.0e-5451.47ortholog of human splicing factor SC35 [more]
AT5G18810.11.1e-1538.52SC35-like splicing factor 28 [more]
AT1G55310.11.8e-1032.00SC35-like splicing factor 33 [more]
AT1G55310.32.7e-0930.80SC35-like splicing factor 33 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 17..90
e-value: 2.6E-24
score: 96.8
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 18..88
e-value: 3.5E-20
score: 71.6
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 16..94
score: 18.990221
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 8..131
e-value: 1.1E-28
score: 102.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 162..229
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 199..229
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 170..191
NoneNo IPR availablePANTHERPTHR23147SERINE/ARGININE RICH SPLICING FACTORcoord: 1..127
NoneNo IPR availablePANTHERPTHR23147:SF161OS08G0486200 PROTEINcoord: 1..127
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 9..120

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG15g02320.1Cp4.1LG15g02320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005622 intracellular anatomical structure
molecular_function GO:0003723 RNA binding
molecular_function GO:0003676 nucleic acid binding