Cp4.1LG14g08930 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG14g08930
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionprotein NRT1/ PTR FAMILY 7.3-like
LocationCp4.1LG14: 7457490 .. 7461669 (+)
RNA-Seq ExpressionCp4.1LG14g08930
SyntenyCp4.1LG14g08930
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GGTAAGTTGAAGGAAGAGATTGCTGCAGTAGAAGAGTTCACTCTAGACGGAACGGTCGATTATCATGGTCGTCCTGCAGTTCGATCAAAATCCGGCTCGTGGGTTGCAGGAATTATAATTCTTCGTGAGTTACCTCTTATCATTTGATACTTTTTTTCGTTTAGTATAATGATAGTAGGAGGTGGGAATCGAGAATCAACGACTTTAGGAATGATTTTTTAAATTATACTTAAAAAGATGTGACAAGATACGAAGATATAACCAATAGAAAGAATCAATAATGTCCCGAACCTTAGGTTAGATCGATGTATTTAATTTACGTGTTTATGTTTACGTGTGAAAAAGGTCACGTAATGAAATTGAAGGCTGAAATTTGGCGACAAGATTGATTGAATATTTTTTAAATAAATAAATAAATAAATAAATAAATAAATAAAAAAAAAAAACATTTATGATATTGAAAAGAGACTCAACAAACAAAGTCTTGTCTTGAAAGTGTGTTTTTTTGAGAGTGATTCATTCTCTTTTGGACAAAGCCATAAATGCCAATTATTATATTAAATATTAAATATTTTTTATTTTTTAATTAAAAAATAAAATTTAAATTTTGGGCTAAATTTATTCTTTTTTTTTTTTTTTCATTTTTAATGCTATATAATATTTGAAAATATATTATTAAATAAGATAATTATATAAATAATTATAAGGTTTAAATTAAATAAATTTATTTTTGTCCTATTGTACGGACGGAGGGCGAAGGGCTAAATTTTTCTCTTGGTCCAAAACGCTGCCACGTGGCAGCATTGGATGGTGGGGAAAAGACTTGAGCCCTAAATTTTGGGCCTAAAGGGAAATGGGCCCCAGCCCACATTATTTGACGTTGAGTTTCGGATGAAAAGGGTATTTAGGTGGCAGCTTGTGAGTGGTGGGTGACGGCTTTTTAATGACAGCCTGATCAAGTTGTTAAATACAAGAAAAATTAAATAAAAATTTAAAAAAATATTGATAAACTGTACCATCCATTTTGAACCACAGTGCACTTGCCTGTCCTAGCTTGGAATCTCAACTTGGGCAATGGAAAATAGACTTTTCGATACGTTCACGTTCACGTTCACGTCTACATCCATGTTTTTTTTAAACCGAACACAGTTGTCTGGTGCTACGATAAGACGTTTCGTGTTTACGACGTCTTTGCTTTTCCTTTTTCTTGAGTGATCTAAGTATTCAGAGAACTGATAAAAGTCGTATAAAATTTAAGGCTTTCTTGTTGTTCGAATATCTGACTTTGTTTAAACCATTTGAAATACGTCCACGTTTTTTTTTTTATCTGGGTTGGGCTTAGTTGACTTTATCTCGTGGGCCAATGATACTTCAATTGTTTATCTTGTGCATAAATTACGATTATATGATTATATAAAGTTTTCTAACTATGGTTCGTTCATGCCAGTGAACCAAGGGTTGGCAACGTTGGCATTTTTTGGAGTCGGAGTAAACTTGGTACTATTTCTAACTAGGGTTTTGCAACAAAGTAACGTTGATGCGGCCAATAACGTCAGCAAATGGACTGGAACTGTTTATATCTTCTCTCTCGTTGGAGCATTCCTCAGTGATTCTTATTGGGGCAGATACAAAACCTGTGCCATCTTTCAAATCATTTTCGTCATCGTAAGTTTTTTTTAAAACCAATTTGAGCATAATTCAATCGACAATGTTCGTTTTTTCAAATTTTTGTGCTAACTTGGTCCGAAAATGGCAGGGCCTAGTGTCGTTATCGCTCTCCTCCTACCTCTTCTTGATCAGACCCAACGGCTGTGGCGATGAACATACGCCCTGTGGAAGTCATTCCAAGACCGAAATTTCCCTCTTCTACCTTTCCATATACCTCACAGCTCTTGGCAATGGCGGCTATCAGCCCAACATCGCCACTTTTGGCGCCGATCAGTTCGACGAAGAGGATCAGAAAGAGGGCCACTATAAGGTCGCTTTCTTCAGCTACTTCTACCTTGCTCTAAACCTTGGATCGCTCTTCTCCAACACAATTTTAGGGTTTTTTGAAGATGAGGGCATGTGGGCTCTTGGGTTCTGGGTCTCCGCCGGATCCGCCATTGCTGCTCTGCTCTTGTTTCTTGCCGGAACTCCACGGTACAGGCATTTCAAACCCTCTGGAAACCCTCTTACGAGGGTTTGTCAAGTCGTTGTCTCCGCCGCGAAGAAATGGCGGGTTAGGGTTCCGTCTGGAGGAGAGGGGTTGTTTGAGGATAATGAGAAGCAAAACCCTAACAATGGCTGCCGGAAGATTCTACACACTAATGGATTCAAGTAAGTGAGAGATTTTTCCATTGCTGAAGTGAAATTTGTGTTTCTGTCCAATTCATATCAGATTTTTAATAAGCATTCCATTAAAAATGTGAATATAATATGAAATCTATCTAAACATAGGTTGGGGATAGTATAGCTTAACTTGGCTTTAGCTGAACTTTGGATCCTGAGTTTTAAGCACTCTGTTTTGTGTTCTTGTTCTGTTGTTTTGCAAAAGCTTTTCTGAAGATAACAAAACAGAACAATCTCTTAAGAATTGTATTTCCTTAGTTTGTTGTCATAGCATTCTGGGGACGTAAATTGTAACCGTCTAAGCCACCGCTAGTAGATATTGTCCTCTTTAGGTTCTCCTTCAAGGTTGTAAAAACGCATATACGAGGGAGAGATTTTCATACCCTTATAAACAATGTTTCGTTCTCCCTCCCAACCAGATCCCACCCCCACTCGTTCCATTCTCGAATTAATGTGGGACACCCCAATCCACCCTCTTTTGGAGGTCAGTGTTTTTGCTGGCACACTGCCCCATGTCCACCCCCTTCAAGATTGAGCCTCCTCGCTGGCACATCGCTCGGTGTCTGGCTTTGATACCATTTGTAGCCGTCCAAGCCCACCGCTAGCAAATATTGTCACCCCCTTTGGGGCTAGTGTCCCCACTGGCACTCGTTCCTTTCTCCAACTAATGTGGGACACCCTAATCCACCCTCCGGAGACCAGTGTCCTTGCTGGCACACTACCCCATGTCCACCCCCTTCGAGATTGAGCCTCCTCGTTGGCACATCGAACCGGTGTCTGGCTTTGATACCATTTGTAACCGTCCAGGCCCACCGCTAGCAAATATTGTCCTCTTTGGGTTTCCCTTCAAAGTTTTTAAAACGCGTCTGCTAGGGAGAGGTTTCCACACCCTTATAAAGAATGTTTTGTTCTCCTACCCAACCGAGTTGGGATTTCACATAAATAGTTCTTTCTCCTTGTTCTTAACTACTGCAAAAATCACAGGTTCTTGGATAAAGCAGCCTACATTTCCTCAAGGGATTCAAGTGATAAAGAGCAAGGAATGATCAACCCATGGCGCCTCTGCCCAATCACACAAGTAGAAGAAGTAAAATGCATTCTAAAACTGATGCCAATCTGGCTCTGCACCATCATCTACTCAGTTGTCTTCACCCAAATGGCCTCTCTCTTCGTCGAGCAAGGAGCAGCCATGAAAACCACCATCTCGAACTTCCACATCCCACCTGCAAGCATGTCAAGCTTCGACATTCTCAGTGTCGCCCTCTTCATCTTCCTCTACCGTCGCGTCCTCGACCCATTTGTTGGAAAACTGAAAAAATCCAGCTCCAAAGGCCTGACCGAGCTGCAAAGAATGGGGGTCGGACTAATCATAGCTGTAATGGCAATGGTCTCAGCAGGCATCGTCGAGTGCTATAGACTCAAATATGCCCAAGCTGACTGCACACACTGTGAAGGATCAAGCTCCTTGAGCATCTTTTGGCAAGTTCCACAATATGCACTGATTGGAGCCTCTGAAGTTTTCATGTATGTGGGTCAGCTTGAATTCTTCAATGCACAAGCTCCTGATGGCCTCAAGAGCTTTGGGAGTGCGCTATGCATGACATCAATCTCTTTAGGGAACTATGTGAGCAGCTTGTTGGTAACAATGGTGATGAAGATTTCAACAGAGGATGACATGCCTGGTTGGATCCCTGGCAACCTCAACAAGGGTCATTTGGACAGGTTCTACTTTCTTCTTGCTGCCTTGACTGTCATTGATTTTGTGATTTACGTTGCCTGTGCTAAATGGTATAAATGCATCAAATTGGAAGACAAATATGAACAAAATGAAGAACTAGAAAGCTTCAAA

mRNA sequence

GGTAAGTTGAAGGAAGAGATTGCTGCAGTAGAAGAGTTCACTCTAGACGGAACGGTCGATTATCATGGTCGTCCTGCAGTTCGATCAAAATCCGGCTCGTGGGTTGCAGGAATTATAATTCTTCTGAACCAAGGGTTGGCAACGTTGGCATTTTTTGGAGTCGGAGTAAACTTGGTACTATTTCTAACTAGGGTTTTGCAACAAAGTAACGTTGATGCGGCCAATAACGTCAGCAAATGGACTGGAACTGTTTATATCTTCTCTCTCGTTGGAGCATTCCTCAGTGATTCTTATTGGGGCAGATACAAAACCTGTGCCATCTTTCAAATCATTTTCGTCATCGGCCTAGTGTCGTTATCGCTCTCCTCCTACCTCTTCTTGATCAGACCCAACGGCTGTGGCGATGAACATACGCCCTGTGGAAGTCATTCCAAGACCGAAATTTCCCTCTTCTACCTTTCCATATACCTCACAGCTCTTGGCAATGGCGGCTATCAGCCCAACATCGCCACTTTTGGCGCCGATCAGTTCGACGAAGAGGATCAGAAAGAGGGCCACTATAAGGTCGCTTTCTTCAGCTACTTCTACCTTGCTCTAAACCTTGGATCGCTCTTCTCCAACACAATTTTAGGGTTTTTTGAAGATGAGGGCATGTGGGCTCTTGGGTTCTGGGTCTCCGCCGGATCCGCCATTGCTGCTCTGCTCTTGTTTCTTGCCGGAACTCCACGGTACAGGCATTTCAAACCCTCTGGAAACCCTCTTACGAGGGTTTGTCAAGTCGTTGTCTCCGCCGCGAAGAAATGGCGGGTTAGGGTTCCGTCTGGAGGAGAGGGGTTGTTTGAGGATAATGAGAAGCAAAACCCTAACAATGGCTGCCGGAAGATTCTACACACTAATGGATTCAAGGATTCAAGTGATAAAGAGCAAGGAATGATCAACCCATGGCGCCTCTGCCCAATCACACAAGTAGAAGAAGTAAAATGCATTCTAAAACTGATGCCAATCTGGCTCTGCACCATCATCTACTCAGTTGTCTTCACCCAAATGGCCTCTCTCTTCGTCGAGCAAGGAGCAGCCATGAAAACCACCATCTCGAACTTCCACATCCCACCTGCAAGCATGTCAAGCTTCGACATTCTCAGTGTCGCCCTCTTCATCTTCCTCTACCGTCGCGTCCTCGACCCATTTGTTGGAAAACTGAAAAAATCCAGCTCCAAAGGCCTGACCGAGCTGCAAAGAATGGGGGTCGGACTAATCATAGCTGTAATGGCAATGGTCTCAGCAGGCATCGTCGAGTGCTATAGACTCAAATATGCCCAAGCTGACTGCACACACTGTGAAGGATCAAGCTCCTTGAGCATCTTTTGGCAAGTTCCACAATATGCACTGATTGGAGCCTCTGAAGTTTTCATGTATGTGGGTCAGCTTGAATTCTTCAATGCACAAGCTCCTGATGGCCTCAAGAGCTTTGGGAGTGCGCTATGCATGACATCAATCTCTTTAGGGAACTATGTGAGCAGCTTGTTGGTAACAATGGTGATGAAGATTTCAACAGAGGATGACATGCCTGGTTGGATCCCTGGCAACCTCAACAAGGGTCATTTGGACAGGTTCTACTTTCTTCTTGCTGCCTTGACTGTCATTGATTTTGTGATTTACGTTGCCTGTGCTAAATGGTATAAATGCATCAAATTGGAAGACAAATATGAACAAAATGAAGAACTAGAAAGCTTCAAA

Coding sequence (CDS)

GGTAAGTTGAAGGAAGAGATTGCTGCAGTAGAAGAGTTCACTCTAGACGGAACGGTCGATTATCATGGTCGTCCTGCAGTTCGATCAAAATCCGGCTCGTGGGTTGCAGGAATTATAATTCTTCTGAACCAAGGGTTGGCAACGTTGGCATTTTTTGGAGTCGGAGTAAACTTGGTACTATTTCTAACTAGGGTTTTGCAACAAAGTAACGTTGATGCGGCCAATAACGTCAGCAAATGGACTGGAACTGTTTATATCTTCTCTCTCGTTGGAGCATTCCTCAGTGATTCTTATTGGGGCAGATACAAAACCTGTGCCATCTTTCAAATCATTTTCGTCATCGGCCTAGTGTCGTTATCGCTCTCCTCCTACCTCTTCTTGATCAGACCCAACGGCTGTGGCGATGAACATACGCCCTGTGGAAGTCATTCCAAGACCGAAATTTCCCTCTTCTACCTTTCCATATACCTCACAGCTCTTGGCAATGGCGGCTATCAGCCCAACATCGCCACTTTTGGCGCCGATCAGTTCGACGAAGAGGATCAGAAAGAGGGCCACTATAAGGTCGCTTTCTTCAGCTACTTCTACCTTGCTCTAAACCTTGGATCGCTCTTCTCCAACACAATTTTAGGGTTTTTTGAAGATGAGGGCATGTGGGCTCTTGGGTTCTGGGTCTCCGCCGGATCCGCCATTGCTGCTCTGCTCTTGTTTCTTGCCGGAACTCCACGGTACAGGCATTTCAAACCCTCTGGAAACCCTCTTACGAGGGTTTGTCAAGTCGTTGTCTCCGCCGCGAAGAAATGGCGGGTTAGGGTTCCGTCTGGAGGAGAGGGGTTGTTTGAGGATAATGAGAAGCAAAACCCTAACAATGGCTGCCGGAAGATTCTACACACTAATGGATTCAAGGATTCAAGTGATAAAGAGCAAGGAATGATCAACCCATGGCGCCTCTGCCCAATCACACAAGTAGAAGAAGTAAAATGCATTCTAAAACTGATGCCAATCTGGCTCTGCACCATCATCTACTCAGTTGTCTTCACCCAAATGGCCTCTCTCTTCGTCGAGCAAGGAGCAGCCATGAAAACCACCATCTCGAACTTCCACATCCCACCTGCAAGCATGTCAAGCTTCGACATTCTCAGTGTCGCCCTCTTCATCTTCCTCTACCGTCGCGTCCTCGACCCATTTGTTGGAAAACTGAAAAAATCCAGCTCCAAAGGCCTGACCGAGCTGCAAAGAATGGGGGTCGGACTAATCATAGCTGTAATGGCAATGGTCTCAGCAGGCATCGTCGAGTGCTATAGACTCAAATATGCCCAAGCTGACTGCACACACTGTGAAGGATCAAGCTCCTTGAGCATCTTTTGGCAAGTTCCACAATATGCACTGATTGGAGCCTCTGAAGTTTTCATGTATGTGGGTCAGCTTGAATTCTTCAATGCACAAGCTCCTGATGGCCTCAAGAGCTTTGGGAGTGCGCTATGCATGACATCAATCTCTTTAGGGAACTATGTGAGCAGCTTGTTGGTAACAATGGTGATGAAGATTTCAACAGAGGATGACATGCCTGGTTGGATCCCTGGCAACCTCAACAAGGGTCATTTGGACAGGTTCTACTTTCTTCTTGCTGCCTTGACTGTCATTGATTTTGTGATTTACGTTGCCTGTGCTAAATGGTATAAATGCATCAAATTGGAAGACAAATATGAACAAAATGAAGAACTAGAAAGCTTCAAA

Protein sequence

GKLKEEIAAVEEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVLFLTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSLSSYLFLIRPNGCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEEDQKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAGTPRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNGFKDSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQMASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGNLNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK
Homology
BLAST of Cp4.1LG14g08930 vs. ExPASy Swiss-Prot
Match: Q9LQL2 (Protein NRT1/ PTR FAMILY 7.3 OS=Arabidopsis thaliana OX=3702 GN=NPF7.3 PE=1 SV=2)

HSP 1 Score: 902.5 bits (2331), Expect = 2.5e-261
Identity = 445/577 (77.12%), Postives = 494/577 (85.62%), Query Frame = 0

Query: 11  EEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVLFLTRVLQQSN 70
           EE T DGTVDY+GRP++RS SG WVAGI+ILLNQGLATLAFFGVGVNLVLFLTRVLQQ+N
Sbjct: 18  EEETRDGTVDYYGRPSIRSNSGQWVAGIVILLNQGLATLAFFGVGVNLVLFLTRVLQQNN 77

Query: 71  VDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSLSSYLFLIRP 130
            DAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQ+IFVIGL SLSLSSY+FLIRP
Sbjct: 78  ADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQVIFVIGLSSLSLSSYMFLIRP 137

Query: 131 NGCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEEDQKEGHYKVA 190
            GCGDE TPCGSHS  EI++FY SIYL ALG GGYQPNIAT GADQFDEE  KEG+ K+A
Sbjct: 138 RGCGDEVTPCGSHSMMEITMFYFSIYLIALGYGGYQPNIATLGADQFDEEHPKEGYSKIA 197

Query: 191 FFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAGTPRYRHFKPS 250
           FFSYFYLALNLGSLFSNTILG+FEDEGMWALGFW S GSAI  L+LFL GTPRYR+FKP+
Sbjct: 198 FFSYFYLALNLGSLFSNTILGYFEDEGMWALGFWASTGSAIIGLILFLVGTPRYRYFKPT 257

Query: 251 GNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPN---NGCRKILHTNGFK----- 310
           GNPL+R CQV+V+A KK  V  P  G     D + +  N   N  R+I+HT+ FK     
Sbjct: 258 GNPLSRFCQVLVAATKKSSVEAPLRGREEMYDGDSEGKNASVNTGRRIVHTDEFKFLDKA 317

Query: 311 ------DSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQMASLFVEQ 370
                 D  DK+Q  +NPWRLCP+TQVEEVKCIL+LMPIWLCTIIYSVVFTQMASLFVEQ
Sbjct: 318 AYITARDLDDKKQDSVNPWRLCPVTQVEEVKCILRLMPIWLCTIIYSVVFTQMASLFVEQ 377

Query: 371 GAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLTELQRMGV 430
           GAAM T++S+F IPPASMSSFDILSVALFIFLYRRVL+P   + KK+ SKG+TEL RMG+
Sbjct: 378 GAAMNTSVSDFKIPPASMSSFDILSVALFIFLYRRVLEPVANRFKKNGSKGITELHRMGI 437

Query: 431 GLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEVFMYVGQL 490
           GL+IAV+AM++AGIVECYRLKYA   CTHC+GSSSLSIFWQ PQY+LIGASEVFMYVGQL
Sbjct: 438 GLVIAVIAMIAAGIVECYRLKYADKSCTHCDGSSSLSIFWQAPQYSLIGASEVFMYVGQL 497

Query: 491 EFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGNLNKGHLD 550
           EFFNAQ PDGLKSFGSALCM S+S+GN+VSSLLVTMV+KISTED MPGWIP NLNKGHLD
Sbjct: 498 EFFNAQTPDGLKSFGSALCMMSMSMGNFVSSLLVTMVVKISTEDHMPGWIPRNLNKGHLD 557

Query: 551 RFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNE 574
           RFYFLLAALT ID V+Y+ACAKWYK I+LE K E  +
Sbjct: 558 RFYFLLAALTSIDLVVYIACAKWYKPIQLEGKDEMQD 594

BLAST of Cp4.1LG14g08930 vs. ExPASy Swiss-Prot
Match: Q8GXN2 (Protein NRT1/ PTR FAMILY 7.2 OS=Arabidopsis thaliana OX=3702 GN=NPF7.2 PE=2 SV=2)

HSP 1 Score: 772.3 bits (1993), Expect = 3.8e-222
Identity = 391/585 (66.84%), Postives = 460/585 (78.63%), Query Frame = 0

Query: 3   LKEEIAAVEEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVLFL 62
           + +++   E  T DG+VD HG PA+R+ +G W+  I+IL+NQGLATLAFFGVGVNLVLFL
Sbjct: 1   MDQKVRQFEVCTQDGSVDRHGNPAIRANTGKWLTAILILVNQGLATLAFFGVGVNLVLFL 60

Query: 63  TRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSLS 122
           TRV+ Q N +AANNVSKWTGTVYIFSL+GAFLSDSYWGRYKTCAIFQ  FV GL+ LSLS
Sbjct: 61  TRVMGQDNAEAANNVSKWTGTVYIFSLLGAFLSDSYWGRYKTCAIFQASFVAGLMMLSLS 120

Query: 123 SYLFLIRPNGCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEEDQ 182
           +   L+ P+GCG E +PC  HS  +  LFYLS+YL ALG GGYQPNIATFGADQFD ED 
Sbjct: 121 TGALLLEPSGCGVEDSPCKPHSTFKTVLFYLSVYLIALGYGGYQPNIATFGADQFDAEDS 180

Query: 183 KEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAGTP 242
            EGH K+AFFSYFYLALNLGSLFSNT+LG+FED+G W LGFW SAGSA A L+LFL GTP
Sbjct: 181 VEGHSKIAFFSYFYLALNLGSLFSNTVLGYFEDQGEWPLGFWASAGSAFAGLVLFLIGTP 240

Query: 243 RYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNGFK 302
           +YRHF P  +P +R CQV+V+A +K ++ V      L+ D+E Q    G +KILHT GF+
Sbjct: 241 KYRHFTPRESPWSRFCQVLVAATRKAKIDVHHEELNLY-DSETQ--YTGDKKILHTKGFR 300

Query: 303 -----------DSSDKEQ--GMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQM 362
                      D ++K +     +PWRLC +TQVEEVKC+L+L+PIWLCTI+YSVVFTQM
Sbjct: 301 FLDRAAIVTPDDEAEKVESGSKYDPWRLCSVTQVEEVKCVLRLLPIWLCTILYSVVFTQM 360

Query: 363 ASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSS-SKGL 422
           ASLFV QGAAMKT I NF IP +SMSSFDILSVA FIF YRR LDP   +L K+  +KGL
Sbjct: 361 ASLFVVQGAAMKTNIKNFRIPASSMSSFDILSVAFFIFAYRRFLDPLFARLNKTERNKGL 420

Query: 423 TELQRMGVGLIIAVMAMVSAGIVECYRLKYAQAD-CTHCEGSSSLSIFWQVPQYALIGAS 482
           TELQRMG+GL+IA+MAM+SAGIVE +RLK  + +  T    SS+LSIFWQVPQY LIGAS
Sbjct: 421 TELQRMGIGLVIAIMAMISAGIVEIHRLKNKEPESATSISSSSTLSIFWQVPQYMLIGAS 480

Query: 483 EVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIP 542
           EVFMYVGQLEFFN+QAP GLKSF SALCM SISLGNYVSSLLV++VMKIST DD+ GWIP
Sbjct: 481 EVFMYVGQLEFFNSQAPTGLKSFASALCMASISLGNYVSSLLVSIVMKISTTDDVHGWIP 540

Query: 543 GNLNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQN 573
            NLNKGHL+RFYFLLA LT  DFV+Y+ CAKWYK IK E  + ++
Sbjct: 541 ENLNKGHLERFYFLLAGLTAADFVVYLICAKWYKYIKSEASFSES 582

BLAST of Cp4.1LG14g08930 vs. ExPASy Swiss-Prot
Match: Q3E9B5 (Protein NRT1/ PTR FAMILY 7.1 OS=Arabidopsis thaliana OX=3702 GN=NPF7.1 PE=2 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 1.8e-179
Identity = 322/547 (58.87%), Postives = 392/547 (71.66%), Query Frame = 0

Query: 30  KSGSWVAGIIILLNQGLATLAFFGVGVNLVLFLTRVLQQSNVDAANNVSKWTGTVYIFSL 89
           K+G W   II+L+NQGLATLAFFGVGVNLVLFLTRV+ Q N +AANNVSKWTGTVY+FSL
Sbjct: 59  KNGGWTNAIILLVNQGLATLAFFGVGVNLVLFLTRVMGQGNAEAANNVSKWTGTVYMFSL 118

Query: 90  VGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSLSSYLFLIRPNGCGDEHTPCGSHSKTEIS 149
           VGAFLSDSYWGRY TC IFQ+IFVIG+  LS  S+ FLI+P GCGD    C   S   ++
Sbjct: 119 VGAFLSDSYWGRYLTCTIFQVIFVIGVGLLSFVSWFFLIKPRGCGDGDLECNPPSSLGVA 178

Query: 150 LFYLSIYLTALGNGGYQPNIATFGADQFDEEDQKEGHYKVAFFSYFYLALNLGSLFSNTI 209
           +FYLS+YL A G GG+QP +ATFGADQ D++       K AFFSYFY ALN+G+LFSNTI
Sbjct: 179 IFYLSVYLVAFGYGGHQPTLATFGADQLDDDKNS----KAAFFSYFYFALNVGALFSNTI 238

Query: 210 LGFFEDEGMWALGFWVSAGSAIAALLLFLAGTPRYRHFKPSGNPLTRVCQVVVSAAKKWR 269
           L +FED+G+W  GF VS GSAI AL+ FLA T +YR+ KP GNPL RV QV V+ A+KW 
Sbjct: 239 LVYFEDKGLWTEGFLVSLGSAIVALVAFLAPTRQYRYVKPCGNPLPRVAQVFVATARKWS 298

Query: 270 VRVPSGGEGLFEDNEKQNPNNGCRKILHTNGFK--------DSSDKEQGMINPWRLCPIT 329
           V  P     L+E    ++   G RKI H+  F           +D+     N WRLC +T
Sbjct: 299 VVRPGDPHELYELEGPESAIKGSRKIFHSTKFLFLDRAAVITENDRNGTRSNAWRLCSVT 358

Query: 330 QVEEVKCILKLMPIWLCTIIYSVVFTQMASLFVEQGAAMKTTISNFHIPPASMSSFDILS 389
           QVEE KC++KL+PIWLCTIIYSV+FTQMASLFVEQG  M   +  FHIP ASMS FDI S
Sbjct: 359 QVEEAKCVMKLLPIWLCTIIYSVIFTQMASLFVEQGDVMNAYVGKFHIPAASMSVFDIFS 418

Query: 390 VALFIFLYRRVLDPFVGKLKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAQA 449
           V +   +YR ++ P+V           TEL RMG+GLII +MAMV+AG+ E  RLK    
Sbjct: 419 VFVSTGIYRHIIFPYVRP---------TELMRMGIGLIIGIMAMVAAGLTEIQRLKRVVP 478

Query: 450 DCTHCEGSSSLSIFWQVPQYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISL 509
                +  S L+I WQ+PQY L+GASEVFMYVGQLEFFN QAPDGLK+ GS+LCM S++L
Sbjct: 479 G----QKESELTILWQIPQYVLVGASEVFMYVGQLEFFNGQAPDGLKNLGSSLCMASMAL 538

Query: 510 GNYVSSLLVTMVMKISTE-DDMPGWIPGNLNKGHLDRFYFLLAALTVIDFVIYVACAKWY 568
           GNYVSSL+V +VM I+   ++ PGWIP NLN+GH+DRFYFL+AAL  IDFV+Y+  AKWY
Sbjct: 539 GNYVSSLMVNIVMAITKRGENSPGWIPENLNEGHMDRFYFLIAALAAIDFVVYLIFAKWY 588

BLAST of Cp4.1LG14g08930 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 2.0e-133
Identity = 240/559 (42.93%), Postives = 372/559 (66.55%), Query Frame = 0

Query: 13  FTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVLFLTRVLQQSNVD 72
           +T DGTVD H  PA + K+G+W A   IL N+    LA++G+G NLV +L   L Q N  
Sbjct: 7   YTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNAT 66

Query: 73  AANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSLSSYLFLIRPNG 132
           AANNV+ W+GT YI  L+GAF++D+Y GRY T A F  I+V G+  L+LS+ +  ++P  
Sbjct: 67  AANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGN 126

Query: 133 CGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEEDQKEGHYKVAFF 192
           C  +   C  +S ++ ++F++++Y+ ALG GG +P +++FGADQFDE D+ E   K +FF
Sbjct: 127 CNAD--TCHPNS-SQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFF 186

Query: 193 SYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAGTPRYRHFKPSGN 252
           ++FY ++N+G+L + T+L + +    W  GF V   + + A+  F  G+  YR  +P G+
Sbjct: 187 NWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGS 246

Query: 253 PLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNGFK---------D 312
           PLTR+ QV+V+A +K  V+VP     LFE  + ++   G RK++HT+  K          
Sbjct: 247 PLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQ 306

Query: 313 SSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQMASLFVEQGAAMKTT 372
           S   + G +NPWRLC +TQVEE+K I+ L+P+W   I+++ V++QM+++FV QG  M   
Sbjct: 307 SDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQH 366

Query: 373 I-SNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLTELQRMGVGLIIAV 432
           +  NF IP AS+S FD +SV  +  +Y + + P   K  + + +G T+LQRMG+GL++++
Sbjct: 367 MGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTR-NERGFTQLQRMGIGLVVSI 426

Query: 433 MAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEVFMYVGQLEFFNAQ 492
            AM++AG++E  RL Y +    + +    +SIFWQ+PQY LIG +EVF ++GQLEFF  Q
Sbjct: 427 FAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQ 486

Query: 493 APDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGNLNKGHLDRFYFLL 552
           APD ++S  SAL +T+++LGNY+S++LVT+VMKI+ ++  PGWIP NLN+GHLD F++LL
Sbjct: 487 APDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYLL 546

Query: 553 AALTVIDFVIYVACAKWYK 562
           A L+ ++F++Y+  +K YK
Sbjct: 547 ATLSFLNFLVYLWISKRYK 561

BLAST of Cp4.1LG14g08930 vs. ExPASy Swiss-Prot
Match: Q93Z20 (Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 4.7e-127
Identity = 232/557 (41.65%), Postives = 357/557 (64.09%), Query Frame = 0

Query: 16  DGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVLFLTRVLQQSNVDAAN 75
           DG++D +G P  + K+G+W A   IL N+    LA++G+  NL+ + T  L +SNV AA+
Sbjct: 38  DGSIDIYGNPPSKKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELHESNVSAAS 97

Query: 76  NVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSLSSYLFLIRPNGC-G 135
           +V  W GT YI  L+GA ++DSYWGRY T A F  I+ IG+  L+LS+ L +++P  C G
Sbjct: 98  DVMIWQGTCYITPLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPVLKPAACAG 157

Query: 136 DEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEEDQKEGHYKVAFFSY 195
                C   +  + ++F+  +YL ALG GG +P +++FGADQFD+ D +E   K +FF++
Sbjct: 158 VAAALCSPATTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDPRERVRKASFFNW 217

Query: 196 FYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAGTPRYRHFKPSGNPL 255
           FY ++N+GS  S+T+L + ++   W LGF +       ++  F  GTP YR  KP G+P+
Sbjct: 218 FYFSINIGSFISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTPLYRFQKPGGSPI 277

Query: 256 TRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNGFK---------DSS 315
           TRVCQV+V+A +K ++ +P     L+E  EK +   G RKI HT+G+K         +  
Sbjct: 278 TRVCQVLVAAYRKLKLNLPEDISFLYETREKNSMIAGSRKIQHTDGYKFLDKAAVISEYE 337

Query: 316 DKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQMASLFVEQGAAMKTTIS 375
            K     NPW+LC +TQVEEVK ++++ PIW   I+YSV+++Q+++LFV+QG +M   I 
Sbjct: 338 SKSGAFSNPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTLFVQQGRSMNRIIR 397

Query: 376 NFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLTELQRMGVGLIIAVMAM 435
           +F IPPAS   FD L V + I +Y R L PFV +      KGLT+LQRMG+GL ++V+++
Sbjct: 398 SFEIPPASFGVFDTLIVLISIPIYDRFLVPFVRRF-TGIPKGLTDLQRMGIGLFLSVLSI 457

Query: 436 VSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEVFMYVGQLEFFNAQAPD 495
            +A IVE  RL+ AQ          ++SIFWQ+PQY L+G +EVF ++G++EFF  ++PD
Sbjct: 458 AAAAIVETVRLQLAQ-------DFVAMSIFWQIPQYILMGIAEVFFFIGRVEFFYDESPD 517

Query: 496 GLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGNLNKGHLDRFYFLLAAL 555
            ++S  SAL + + ++G+Y+SSL++T+V   +      GW+P +LNKGHLD F++LL +L
Sbjct: 518 AMRSVCSALALLNTAVGSYLSSLILTLVAYFTALGGKDGWVPDDLNKGHLDYFFWLLVSL 577

Query: 556 TVIDFVIY-VACAKWYK 562
            +++  +Y + C K  K
Sbjct: 578 GLVNIPVYALICVKHTK 586

BLAST of Cp4.1LG14g08930 vs. NCBI nr
Match: XP_023553282.1 (protein NRT1/ PTR FAMILY 7.3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1153 bits (2982), Expect = 0.0
Identity = 579/590 (98.14%), Postives = 579/590 (98.14%), Query Frame = 0

Query: 1   GKLKEEIAAVEEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVL 60
           GKLKEEIAAVEEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVL
Sbjct: 11  GKLKEEIAAVEEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVL 70

Query: 61  FLTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLS 120
           FLTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLS
Sbjct: 71  FLTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLS 130

Query: 121 LSSYLFLIRPNGCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEE 180
           LSSYLFLIRPNGCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEE
Sbjct: 131 LSSYLFLIRPNGCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEE 190

Query: 181 DQKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAG 240
           DQKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAG
Sbjct: 191 DQKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAG 250

Query: 241 TPRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNG 300
           TPRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNG
Sbjct: 251 TPRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNG 310

Query: 301 FK-----------DSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQM 360
           FK           DSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQM
Sbjct: 311 FKFLDKAAYISSRDSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQM 370

Query: 361 ASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLT 420
           ASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLT
Sbjct: 371 ASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLT 430

Query: 421 ELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEV 480
           ELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEV
Sbjct: 431 ELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEV 490

Query: 481 FMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGN 540
           FMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGN
Sbjct: 491 FMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGN 550

Query: 541 LNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK 579
           LNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK
Sbjct: 551 LNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK 600

BLAST of Cp4.1LG14g08930 vs. NCBI nr
Match: XP_022923413.1 (protein NRT1/ PTR FAMILY 7.3-like [Cucurbita moschata])

HSP 1 Score: 1149 bits (2972), Expect = 0.0
Identity = 577/590 (97.80%), Postives = 577/590 (97.80%), Query Frame = 0

Query: 1   GKLKEEIAAVEEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVL 60
           GKLKEEIA VEEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVL
Sbjct: 11  GKLKEEIATVEEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVL 70

Query: 61  FLTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLS 120
           FLTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLS
Sbjct: 71  FLTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLS 130

Query: 121 LSSYLFLIRPNGCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEE 180
           LSSYLFLIRP GCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEE
Sbjct: 131 LSSYLFLIRPKGCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEE 190

Query: 181 DQKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAG 240
           DQKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAG
Sbjct: 191 DQKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAG 250

Query: 241 TPRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNG 300
           TPRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNG
Sbjct: 251 TPRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNG 310

Query: 301 FK-----------DSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQM 360
           FK           DSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQM
Sbjct: 311 FKFLDKAAYISSRDSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQM 370

Query: 361 ASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLT 420
           ASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLT
Sbjct: 371 ASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLT 430

Query: 421 ELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEV 480
           ELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEV
Sbjct: 431 ELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEV 490

Query: 481 FMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGN 540
           FMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGN
Sbjct: 491 FMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGN 550

Query: 541 LNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK 579
           LNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK
Sbjct: 551 LNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK 600

BLAST of Cp4.1LG14g08930 vs. NCBI nr
Match: KAG6577630.1 (Protein NRT1/ PTR FAMILY 7.3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015672.1 Protein NRT1/ PTR FAMILY 7.3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1148 bits (2969), Expect = 0.0
Identity = 576/590 (97.63%), Postives = 577/590 (97.80%), Query Frame = 0

Query: 1   GKLKEEIAAVEEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVL 60
           GKLKEEIA VEEFTLDGT+DYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVL
Sbjct: 11  GKLKEEIATVEEFTLDGTLDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVL 70

Query: 61  FLTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLS 120
           FLTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLS
Sbjct: 71  FLTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLS 130

Query: 121 LSSYLFLIRPNGCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEE 180
           LSSYLFLIRP GCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEE
Sbjct: 131 LSSYLFLIRPKGCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEE 190

Query: 181 DQKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAG 240
           DQKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAG
Sbjct: 191 DQKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAG 250

Query: 241 TPRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNG 300
           TPRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNG
Sbjct: 251 TPRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNG 310

Query: 301 FK-----------DSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQM 360
           FK           DSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQM
Sbjct: 311 FKFLDKAAYISSRDSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQM 370

Query: 361 ASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLT 420
           ASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLT
Sbjct: 371 ASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLT 430

Query: 421 ELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEV 480
           ELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEV
Sbjct: 431 ELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEV 490

Query: 481 FMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGN 540
           FMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGN
Sbjct: 491 FMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGN 550

Query: 541 LNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK 579
           LNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK
Sbjct: 551 LNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK 600

BLAST of Cp4.1LG14g08930 vs. NCBI nr
Match: XP_023007750.1 (protein NRT1/ PTR FAMILY 7.3-like [Cucurbita maxima])

HSP 1 Score: 1143 bits (2956), Expect = 0.0
Identity = 575/590 (97.46%), Postives = 576/590 (97.63%), Query Frame = 0

Query: 1   GKLKEEIAAVEEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVL 60
           GKLKEEIAAVEEFTLDGTV+YHG PAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVL
Sbjct: 11  GKLKEEIAAVEEFTLDGTVNYHGHPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVL 70

Query: 61  FLTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLS 120
           FLTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLS
Sbjct: 71  FLTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLS 130

Query: 121 LSSYLFLIRPNGCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEE 180
           LSSYLFLIRP GCGDEHT CGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEE
Sbjct: 131 LSSYLFLIRPKGCGDEHTLCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEE 190

Query: 181 DQKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAG 240
           DQKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAG
Sbjct: 191 DQKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAG 250

Query: 241 TPRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNG 300
           TPRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNG
Sbjct: 251 TPRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNG 310

Query: 301 FK-----------DSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQM 360
           FK           DSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQM
Sbjct: 311 FKFLDKAAYISSRDSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQM 370

Query: 361 ASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLT 420
           ASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLT
Sbjct: 371 ASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLT 430

Query: 421 ELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEV 480
           ELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEV
Sbjct: 431 ELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEV 490

Query: 481 FMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGN 540
           FMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGN
Sbjct: 491 FMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGN 550

Query: 541 LNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK 579
           LNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK
Sbjct: 551 LNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK 600

BLAST of Cp4.1LG14g08930 vs. NCBI nr
Match: XP_038906251.1 (protein NRT1/ PTR FAMILY 7.3-like [Benincasa hispida])

HSP 1 Score: 1079 bits (2790), Expect = 0.0
Identity = 542/589 (92.02%), Postives = 558/589 (94.74%), Query Frame = 0

Query: 2   KLKEEIAAVEEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVLF 61
           KLKEEIAA E FTLDGTVD+HGRPA+RSKSG+WVAGIIILLNQGLATLAFFGVGVNLVLF
Sbjct: 12  KLKEEIAAAEGFTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLAFFGVGVNLVLF 71

Query: 62  LTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSL 121
           LTRVLQQ+N DAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSL
Sbjct: 72  LTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSL 131

Query: 122 SSYLFLIRPNGCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEED 181
           SSYLFLIRP GCGDE TPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEE 
Sbjct: 132 SSYLFLIRPKGCGDEQTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEEY 191

Query: 182 QKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAGT 241
           QKEGH KVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVS GSA+AALLLFL GT
Sbjct: 192 QKEGHSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSTGSAVAALLLFLIGT 251

Query: 242 PRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNGF 301
           PRYRHFKP+GNPLTRV QVV+SAAKKWRV VPS GEGLF+D+ KQ+PNNGCRKILHT+GF
Sbjct: 252 PRYRHFKPTGNPLTRVSQVVISAAKKWRVMVPSDGEGLFDDDGKQSPNNGCRKILHTHGF 311

Query: 302 K-----------DSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQMA 361
           K           D SDKEQG+ NPWRLCPITQVEEVKCIL+L+PIWLCTIIYSVVFTQMA
Sbjct: 312 KFLDKAAYISSRDLSDKEQGVNNPWRLCPITQVEEVKCILRLLPIWLCTIIYSVVFTQMA 371

Query: 362 SLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLTE 421
           SLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLTE
Sbjct: 372 SLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLTE 431

Query: 422 LQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEVF 481
           LQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEVF
Sbjct: 432 LQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEVF 491

Query: 482 MYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGNL 541
           MYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKIST D MPGWIPGNL
Sbjct: 492 MYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTVDRMPGWIPGNL 551

Query: 542 NKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK 579
           NKGHLDRFYFLLAALTVIDFVIY+ACAKWYK IKLE+KYEQ EE ESFK
Sbjct: 552 NKGHLDRFYFLLAALTVIDFVIYIACAKWYKSIKLEEKYEQTEEQESFK 600

BLAST of Cp4.1LG14g08930 vs. ExPASy TrEMBL
Match: A0A6J1E9L0 (protein NRT1/ PTR FAMILY 7.3-like OS=Cucurbita moschata OX=3662 GN=LOC111431116 PE=3 SV=1)

HSP 1 Score: 1149 bits (2972), Expect = 0.0
Identity = 577/590 (97.80%), Postives = 577/590 (97.80%), Query Frame = 0

Query: 1   GKLKEEIAAVEEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVL 60
           GKLKEEIA VEEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVL
Sbjct: 11  GKLKEEIATVEEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVL 70

Query: 61  FLTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLS 120
           FLTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLS
Sbjct: 71  FLTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLS 130

Query: 121 LSSYLFLIRPNGCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEE 180
           LSSYLFLIRP GCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEE
Sbjct: 131 LSSYLFLIRPKGCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEE 190

Query: 181 DQKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAG 240
           DQKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAG
Sbjct: 191 DQKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAG 250

Query: 241 TPRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNG 300
           TPRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNG
Sbjct: 251 TPRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNG 310

Query: 301 FK-----------DSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQM 360
           FK           DSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQM
Sbjct: 311 FKFLDKAAYISSRDSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQM 370

Query: 361 ASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLT 420
           ASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLT
Sbjct: 371 ASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLT 430

Query: 421 ELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEV 480
           ELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEV
Sbjct: 431 ELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEV 490

Query: 481 FMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGN 540
           FMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGN
Sbjct: 491 FMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGN 550

Query: 541 LNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK 579
           LNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK
Sbjct: 551 LNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK 600

BLAST of Cp4.1LG14g08930 vs. ExPASy TrEMBL
Match: A0A6J1L3U9 (protein NRT1/ PTR FAMILY 7.3-like OS=Cucurbita maxima OX=3661 GN=LOC111500269 PE=3 SV=1)

HSP 1 Score: 1143 bits (2956), Expect = 0.0
Identity = 575/590 (97.46%), Postives = 576/590 (97.63%), Query Frame = 0

Query: 1   GKLKEEIAAVEEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVL 60
           GKLKEEIAAVEEFTLDGTV+YHG PAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVL
Sbjct: 11  GKLKEEIAAVEEFTLDGTVNYHGHPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVL 70

Query: 61  FLTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLS 120
           FLTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLS
Sbjct: 71  FLTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLS 130

Query: 121 LSSYLFLIRPNGCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEE 180
           LSSYLFLIRP GCGDEHT CGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEE
Sbjct: 131 LSSYLFLIRPKGCGDEHTLCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEE 190

Query: 181 DQKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAG 240
           DQKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAG
Sbjct: 191 DQKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAG 250

Query: 241 TPRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNG 300
           TPRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNG
Sbjct: 251 TPRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNG 310

Query: 301 FK-----------DSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQM 360
           FK           DSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQM
Sbjct: 311 FKFLDKAAYISSRDSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQM 370

Query: 361 ASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLT 420
           ASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLT
Sbjct: 371 ASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLT 430

Query: 421 ELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEV 480
           ELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEV
Sbjct: 431 ELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEV 490

Query: 481 FMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGN 540
           FMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGN
Sbjct: 491 FMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGN 550

Query: 541 LNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK 579
           LNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK
Sbjct: 551 LNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK 600

BLAST of Cp4.1LG14g08930 vs. ExPASy TrEMBL
Match: A0A1S3BKC8 (protein NRT1/ PTR FAMILY 7.3-like OS=Cucumis melo OX=3656 GN=LOC103490806 PE=3 SV=1)

HSP 1 Score: 1071 bits (2771), Expect = 0.0
Identity = 538/589 (91.34%), Postives = 556/589 (94.40%), Query Frame = 0

Query: 2   KLKEEIAAVEEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVLF 61
           KLKEEIA  E FTLDGTVD+HGRPA+RSKSG+WVAGIIILLNQGLATLAFFGVGVNLVLF
Sbjct: 12  KLKEEIATAEGFTLDGTVDFHGRPAIRSKSGTWVAGIIILLNQGLATLAFFGVGVNLVLF 71

Query: 62  LTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSL 121
           LTRVLQQ+N DAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSL
Sbjct: 72  LTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSL 131

Query: 122 SSYLFLIRPNGCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEED 181
           SS+LFLIRP GCGDE TPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEE 
Sbjct: 132 SSHLFLIRPKGCGDEQTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEEY 191

Query: 182 QKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAGT 241
           QKEGH KVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSA AALLLFL GT
Sbjct: 192 QKEGHSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAAAALLLFLIGT 251

Query: 242 PRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNGF 301
           PRYRHFKP+GNPLTRV QVVVSAAKKWRV+VPSGGEGLF+D+ K++ NNGCRKILHT+GF
Sbjct: 252 PRYRHFKPTGNPLTRVSQVVVSAAKKWRVKVPSGGEGLFDDDGKESSNNGCRKILHTHGF 311

Query: 302 K-----------DSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQMA 361
           K           D SDKEQG+ NPWRLCPITQVEEVKCIL+L+PIWLCTIIYSVVFTQMA
Sbjct: 312 KFLDKAAYISSRDLSDKEQGVNNPWRLCPITQVEEVKCILRLLPIWLCTIIYSVVFTQMA 371

Query: 362 SLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLTE 421
           SLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSS GLTE
Sbjct: 372 SLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSTGLTE 431

Query: 422 LQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEVF 481
           LQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEVF
Sbjct: 432 LQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEVF 491

Query: 482 MYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGNL 541
           MYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKIST D MPGWIPGNL
Sbjct: 492 MYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTVDRMPGWIPGNL 551

Query: 542 NKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK 579
           NKGHLDRFYFLLAALTV+DFVIY+ CAKWYK IKLE+KYEQ EE ESFK
Sbjct: 552 NKGHLDRFYFLLAALTVVDFVIYIVCAKWYKSIKLEEKYEQTEEQESFK 600

BLAST of Cp4.1LG14g08930 vs. ExPASy TrEMBL
Match: A0A6J1DMR4 (protein NRT1/ PTR FAMILY 7.3-like OS=Momordica charantia OX=3673 GN=LOC111022668 PE=3 SV=1)

HSP 1 Score: 1068 bits (2763), Expect = 0.0
Identity = 535/589 (90.83%), Postives = 558/589 (94.74%), Query Frame = 0

Query: 2   KLKEEIAAVEEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVLF 61
           KLKEE  A EEFTLDGTVD+HGRPA+RSKSG+WVAGIIILLNQGLATLAFFGVGVNLVLF
Sbjct: 12  KLKEEAVAAEEFTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLAFFGVGVNLVLF 71

Query: 62  LTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSL 121
           LTRVLQQ+N DAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSL
Sbjct: 72  LTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSL 131

Query: 122 SSYLFLIRPNGCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEED 181
           SS+LFLIRP GCGD  TPCG+HSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEED
Sbjct: 132 SSHLFLIRPKGCGDLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEED 191

Query: 182 QKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAGT 241
            KEG+ KVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSA SA+AALLLFLAGT
Sbjct: 192 PKEGYSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASAVAALLLFLAGT 251

Query: 242 PRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNGF 301
            RYRHFKP+GNPLTRVCQVVVSAAKKWRVRVPSGGEGLFED+ K++P+NGCRKILHT+GF
Sbjct: 252 SRYRHFKPTGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDDGKRSPDNGCRKILHTHGF 311

Query: 302 K-----------DSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQMA 361
           K           D SD+EQG+ NPWR+CPITQVEEVKCIL+L+PIWLCTIIYSVVFTQMA
Sbjct: 312 KFLDKAAYISSRDLSDQEQGVCNPWRICPITQVEEVKCILRLLPIWLCTIIYSVVFTQMA 371

Query: 362 SLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLTE 421
           SLFVEQGAAMKTTIS+F IPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLTE
Sbjct: 372 SLFVEQGAAMKTTISSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLTE 431

Query: 422 LQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEVF 481
           LQRMGVGLIIAVMAMVSAGIVECYRLKYA+ADCTHCEGSSSLSIFWQVPQYALIGASEVF
Sbjct: 432 LQRMGVGLIIAVMAMVSAGIVECYRLKYAKADCTHCEGSSSLSIFWQVPQYALIGASEVF 491

Query: 482 MYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGNL 541
           MYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKIST D MPGWIPGNL
Sbjct: 492 MYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTVDRMPGWIPGNL 551

Query: 542 NKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK 579
           NKGHLDRFYFLLAALTV+DFVIY+ACAKWYK IKLEDKYEQ EE ESFK
Sbjct: 552 NKGHLDRFYFLLAALTVVDFVIYIACAKWYKSIKLEDKYEQTEEPESFK 600

BLAST of Cp4.1LG14g08930 vs. ExPASy TrEMBL
Match: A0A0A0L1B9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G015860 PE=3 SV=1)

HSP 1 Score: 1063 bits (2750), Expect = 0.0
Identity = 532/589 (90.32%), Postives = 554/589 (94.06%), Query Frame = 0

Query: 2   KLKEEIAAVEEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVLF 61
           KLKEEIA  E FTLDGTVD+HGRPA+RSKSG+WVAGIIILLNQGLATLAFFGVGVNLVLF
Sbjct: 12  KLKEEIATAEGFTLDGTVDFHGRPAIRSKSGTWVAGIIILLNQGLATLAFFGVGVNLVLF 71

Query: 62  LTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSL 121
           LTRVLQQ+N DAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSL
Sbjct: 72  LTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSL 131

Query: 122 SSYLFLIRPNGCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEED 181
           SS+LFLIRP GCGDE TPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEE 
Sbjct: 132 SSHLFLIRPKGCGDEQTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEEY 191

Query: 182 QKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAGT 241
           QKEGH KVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVS GSA AALLLFL GT
Sbjct: 192 QKEGHSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSTGSAAAALLLFLIGT 251

Query: 242 PRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNGF 301
           PRYR+FKP+GNPL RV QVVVSAAKKWR++VPSGGEGLF+D+ K++ NNGCRKILHT+GF
Sbjct: 252 PRYRYFKPTGNPLMRVSQVVVSAAKKWRIKVPSGGEGLFDDDGKESSNNGCRKILHTHGF 311

Query: 302 K-----------DSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQMA 361
           K           D SDKEQG+ NPWRLCPITQVEEVKCIL+L+PIWLCTIIYSVVFTQMA
Sbjct: 312 KFLDKAAYISSRDLSDKEQGVNNPWRLCPITQVEEVKCILRLLPIWLCTIIYSVVFTQMA 371

Query: 362 SLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLTE 421
           SLFVEQGAAMKTT+SNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSS GLTE
Sbjct: 372 SLFVEQGAAMKTTVSNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSTGLTE 431

Query: 422 LQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEVF 481
           LQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEVF
Sbjct: 432 LQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEVF 491

Query: 482 MYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGNL 541
           MYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKIST D MPGWIPGNL
Sbjct: 492 MYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTVDRMPGWIPGNL 551

Query: 542 NKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK 579
           NKGHLDRFYFLLAALTV+DFVIY+ CAKWYK IKLE+KYEQ EE E+FK
Sbjct: 552 NKGHLDRFYFLLAALTVVDFVIYIVCAKWYKSIKLEEKYEQTEEQENFK 600

BLAST of Cp4.1LG14g08930 vs. TAIR 10
Match: AT1G32450.1 (nitrate transporter 1.5 )

HSP 1 Score: 902.5 bits (2331), Expect = 1.7e-262
Identity = 445/577 (77.12%), Postives = 494/577 (85.62%), Query Frame = 0

Query: 11  EEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVLFLTRVLQQSN 70
           EE T DGTVDY+GRP++RS SG WVAGI+ILLNQGLATLAFFGVGVNLVLFLTRVLQQ+N
Sbjct: 18  EEETRDGTVDYYGRPSIRSNSGQWVAGIVILLNQGLATLAFFGVGVNLVLFLTRVLQQNN 77

Query: 71  VDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSLSSYLFLIRP 130
            DAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQ+IFVIGL SLSLSSY+FLIRP
Sbjct: 78  ADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQVIFVIGLSSLSLSSYMFLIRP 137

Query: 131 NGCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEEDQKEGHYKVA 190
            GCGDE TPCGSHS  EI++FY SIYL ALG GGYQPNIAT GADQFDEE  KEG+ K+A
Sbjct: 138 RGCGDEVTPCGSHSMMEITMFYFSIYLIALGYGGYQPNIATLGADQFDEEHPKEGYSKIA 197

Query: 191 FFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAGTPRYRHFKPS 250
           FFSYFYLALNLGSLFSNTILG+FEDEGMWALGFW S GSAI  L+LFL GTPRYR+FKP+
Sbjct: 198 FFSYFYLALNLGSLFSNTILGYFEDEGMWALGFWASTGSAIIGLILFLVGTPRYRYFKPT 257

Query: 251 GNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPN---NGCRKILHTNGFK----- 310
           GNPL+R CQV+V+A KK  V  P  G     D + +  N   N  R+I+HT+ FK     
Sbjct: 258 GNPLSRFCQVLVAATKKSSVEAPLRGREEMYDGDSEGKNASVNTGRRIVHTDEFKFLDKA 317

Query: 311 ------DSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQMASLFVEQ 370
                 D  DK+Q  +NPWRLCP+TQVEEVKCIL+LMPIWLCTIIYSVVFTQMASLFVEQ
Sbjct: 318 AYITARDLDDKKQDSVNPWRLCPVTQVEEVKCILRLMPIWLCTIIYSVVFTQMASLFVEQ 377

Query: 371 GAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLTELQRMGV 430
           GAAM T++S+F IPPASMSSFDILSVALFIFLYRRVL+P   + KK+ SKG+TEL RMG+
Sbjct: 378 GAAMNTSVSDFKIPPASMSSFDILSVALFIFLYRRVLEPVANRFKKNGSKGITELHRMGI 437

Query: 431 GLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEVFMYVGQL 490
           GL+IAV+AM++AGIVECYRLKYA   CTHC+GSSSLSIFWQ PQY+LIGASEVFMYVGQL
Sbjct: 438 GLVIAVIAMIAAGIVECYRLKYADKSCTHCDGSSSLSIFWQAPQYSLIGASEVFMYVGQL 497

Query: 491 EFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGNLNKGHLD 550
           EFFNAQ PDGLKSFGSALCM S+S+GN+VSSLLVTMV+KISTED MPGWIP NLNKGHLD
Sbjct: 498 EFFNAQTPDGLKSFGSALCMMSMSMGNFVSSLLVTMVVKISTEDHMPGWIPRNLNKGHLD 557

Query: 551 RFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNE 574
           RFYFLLAALT ID V+Y+ACAKWYK I+LE K E  +
Sbjct: 558 RFYFLLAALTSIDLVVYIACAKWYKPIQLEGKDEMQD 594

BLAST of Cp4.1LG14g08930 vs. TAIR 10
Match: AT4G21680.1 (NITRATE TRANSPORTER 1.8 )

HSP 1 Score: 772.3 bits (1993), Expect = 2.7e-223
Identity = 391/585 (66.84%), Postives = 460/585 (78.63%), Query Frame = 0

Query: 3   LKEEIAAVEEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVLFL 62
           + +++   E  T DG+VD HG PA+R+ +G W+  I+IL+NQGLATLAFFGVGVNLVLFL
Sbjct: 1   MDQKVRQFEVCTQDGSVDRHGNPAIRANTGKWLTAILILVNQGLATLAFFGVGVNLVLFL 60

Query: 63  TRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSLS 122
           TRV+ Q N +AANNVSKWTGTVYIFSL+GAFLSDSYWGRYKTCAIFQ  FV GL+ LSLS
Sbjct: 61  TRVMGQDNAEAANNVSKWTGTVYIFSLLGAFLSDSYWGRYKTCAIFQASFVAGLMMLSLS 120

Query: 123 SYLFLIRPNGCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEEDQ 182
           +   L+ P+GCG E +PC  HS  +  LFYLS+YL ALG GGYQPNIATFGADQFD ED 
Sbjct: 121 TGALLLEPSGCGVEDSPCKPHSTFKTVLFYLSVYLIALGYGGYQPNIATFGADQFDAEDS 180

Query: 183 KEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAGTP 242
            EGH K+AFFSYFYLALNLGSLFSNT+LG+FED+G W LGFW SAGSA A L+LFL GTP
Sbjct: 181 VEGHSKIAFFSYFYLALNLGSLFSNTVLGYFEDQGEWPLGFWASAGSAFAGLVLFLIGTP 240

Query: 243 RYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNGFK 302
           +YRHF P  +P +R CQV+V+A +K ++ V      L+ D+E Q    G +KILHT GF+
Sbjct: 241 KYRHFTPRESPWSRFCQVLVAATRKAKIDVHHEELNLY-DSETQ--YTGDKKILHTKGFR 300

Query: 303 -----------DSSDKEQ--GMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQM 362
                      D ++K +     +PWRLC +TQVEEVKC+L+L+PIWLCTI+YSVVFTQM
Sbjct: 301 FLDRAAIVTPDDEAEKVESGSKYDPWRLCSVTQVEEVKCVLRLLPIWLCTILYSVVFTQM 360

Query: 363 ASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSS-SKGL 422
           ASLFV QGAAMKT I NF IP +SMSSFDILSVA FIF YRR LDP   +L K+  +KGL
Sbjct: 361 ASLFVVQGAAMKTNIKNFRIPASSMSSFDILSVAFFIFAYRRFLDPLFARLNKTERNKGL 420

Query: 423 TELQRMGVGLIIAVMAMVSAGIVECYRLKYAQAD-CTHCEGSSSLSIFWQVPQYALIGAS 482
           TELQRMG+GL+IA+MAM+SAGIVE +RLK  + +  T    SS+LSIFWQVPQY LIGAS
Sbjct: 421 TELQRMGIGLVIAIMAMISAGIVEIHRLKNKEPESATSISSSSTLSIFWQVPQYMLIGAS 480

Query: 483 EVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIP 542
           EVFMYVGQLEFFN+QAP GLKSF SALCM SISLGNYVSSLLV++VMKIST DD+ GWIP
Sbjct: 481 EVFMYVGQLEFFNSQAPTGLKSFASALCMASISLGNYVSSLLVSIVMKISTTDDVHGWIP 540

Query: 543 GNLNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQN 573
            NLNKGHL+RFYFLLA LT  DFV+Y+ CAKWYK IK E  + ++
Sbjct: 541 ENLNKGHLERFYFLLAGLTAADFVVYLICAKWYKYIKSEASFSES 582

BLAST of Cp4.1LG14g08930 vs. TAIR 10
Match: AT5G19640.1 (Major facilitator superfamily protein )

HSP 1 Score: 630.6 bits (1625), Expect = 1.3e-180
Identity = 322/547 (58.87%), Postives = 392/547 (71.66%), Query Frame = 0

Query: 30  KSGSWVAGIIILLNQGLATLAFFGVGVNLVLFLTRVLQQSNVDAANNVSKWTGTVYIFSL 89
           K+G W   II+L+NQGLATLAFFGVGVNLVLFLTRV+ Q N +AANNVSKWTGTVY+FSL
Sbjct: 59  KNGGWTNAIILLVNQGLATLAFFGVGVNLVLFLTRVMGQGNAEAANNVSKWTGTVYMFSL 118

Query: 90  VGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSLSSYLFLIRPNGCGDEHTPCGSHSKTEIS 149
           VGAFLSDSYWGRY TC IFQ+IFVIG+  LS  S+ FLI+P GCGD    C   S   ++
Sbjct: 119 VGAFLSDSYWGRYLTCTIFQVIFVIGVGLLSFVSWFFLIKPRGCGDGDLECNPPSSLGVA 178

Query: 150 LFYLSIYLTALGNGGYQPNIATFGADQFDEEDQKEGHYKVAFFSYFYLALNLGSLFSNTI 209
           +FYLS+YL A G GG+QP +ATFGADQ D++       K AFFSYFY ALN+G+LFSNTI
Sbjct: 179 IFYLSVYLVAFGYGGHQPTLATFGADQLDDDKNS----KAAFFSYFYFALNVGALFSNTI 238

Query: 210 LGFFEDEGMWALGFWVSAGSAIAALLLFLAGTPRYRHFKPSGNPLTRVCQVVVSAAKKWR 269
           L +FED+G+W  GF VS GSAI AL+ FLA T +YR+ KP GNPL RV QV V+ A+KW 
Sbjct: 239 LVYFEDKGLWTEGFLVSLGSAIVALVAFLAPTRQYRYVKPCGNPLPRVAQVFVATARKWS 298

Query: 270 VRVPSGGEGLFEDNEKQNPNNGCRKILHTNGFK--------DSSDKEQGMINPWRLCPIT 329
           V  P     L+E    ++   G RKI H+  F           +D+     N WRLC +T
Sbjct: 299 VVRPGDPHELYELEGPESAIKGSRKIFHSTKFLFLDRAAVITENDRNGTRSNAWRLCSVT 358

Query: 330 QVEEVKCILKLMPIWLCTIIYSVVFTQMASLFVEQGAAMKTTISNFHIPPASMSSFDILS 389
           QVEE KC++KL+PIWLCTIIYSV+FTQMASLFVEQG  M   +  FHIP ASMS FDI S
Sbjct: 359 QVEEAKCVMKLLPIWLCTIIYSVIFTQMASLFVEQGDVMNAYVGKFHIPAASMSVFDIFS 418

Query: 390 VALFIFLYRRVLDPFVGKLKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAQA 449
           V +   +YR ++ P+V           TEL RMG+GLII +MAMV+AG+ E  RLK    
Sbjct: 419 VFVSTGIYRHIIFPYVRP---------TELMRMGIGLIIGIMAMVAAGLTEIQRLKRVVP 478

Query: 450 DCTHCEGSSSLSIFWQVPQYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISL 509
                +  S L+I WQ+PQY L+GASEVFMYVGQLEFFN QAPDGLK+ GS+LCM S++L
Sbjct: 479 G----QKESELTILWQIPQYVLVGASEVFMYVGQLEFFNGQAPDGLKNLGSSLCMASMAL 538

Query: 510 GNYVSSLLVTMVMKISTE-DDMPGWIPGNLNKGHLDRFYFLLAALTVIDFVIYVACAKWY 568
           GNYVSSL+V +VM I+   ++ PGWIP NLN+GH+DRFYFL+AAL  IDFV+Y+  AKWY
Sbjct: 539 GNYVSSLMVNIVMAITKRGENSPGWIPENLNEGHMDRFYFLIAALAAIDFVVYLIFAKWY 588

BLAST of Cp4.1LG14g08930 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 477.6 bits (1228), Expect = 1.4e-134
Identity = 240/559 (42.93%), Postives = 372/559 (66.55%), Query Frame = 0

Query: 13  FTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVLFLTRVLQQSNVD 72
           +T DGTVD H  PA + K+G+W A   IL N+    LA++G+G NLV +L   L Q N  
Sbjct: 7   YTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNAT 66

Query: 73  AANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSLSSYLFLIRPNG 132
           AANNV+ W+GT YI  L+GAF++D+Y GRY T A F  I+V G+  L+LS+ +  ++P  
Sbjct: 67  AANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGN 126

Query: 133 CGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEEDQKEGHYKVAFF 192
           C  +   C  +S ++ ++F++++Y+ ALG GG +P +++FGADQFDE D+ E   K +FF
Sbjct: 127 CNAD--TCHPNS-SQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFF 186

Query: 193 SYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAGTPRYRHFKPSGN 252
           ++FY ++N+G+L + T+L + +    W  GF V   + + A+  F  G+  YR  +P G+
Sbjct: 187 NWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGS 246

Query: 253 PLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNGFK---------D 312
           PLTR+ QV+V+A +K  V+VP     LFE  + ++   G RK++HT+  K          
Sbjct: 247 PLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQ 306

Query: 313 SSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQMASLFVEQGAAMKTT 372
           S   + G +NPWRLC +TQVEE+K I+ L+P+W   I+++ V++QM+++FV QG  M   
Sbjct: 307 SDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQH 366

Query: 373 I-SNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLTELQRMGVGLIIAV 432
           +  NF IP AS+S FD +SV  +  +Y + + P   K  + + +G T+LQRMG+GL++++
Sbjct: 367 MGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTR-NERGFTQLQRMGIGLVVSI 426

Query: 433 MAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEVFMYVGQLEFFNAQ 492
            AM++AG++E  RL Y +    + +    +SIFWQ+PQY LIG +EVF ++GQLEFF  Q
Sbjct: 427 FAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQ 486

Query: 493 APDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGNLNKGHLDRFYFLL 552
           APD ++S  SAL +T+++LGNY+S++LVT+VMKI+ ++  PGWIP NLN+GHLD F++LL
Sbjct: 487 APDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYLL 546

Query: 553 AALTVIDFVIYVACAKWYK 562
           A L+ ++F++Y+  +K YK
Sbjct: 547 ATLSFLNFLVYLWISKRYK 561

BLAST of Cp4.1LG14g08930 vs. TAIR 10
Match: AT1G62200.1 (Major facilitator superfamily protein )

HSP 1 Score: 456.4 bits (1173), Expect = 3.3e-128
Identity = 232/557 (41.65%), Postives = 357/557 (64.09%), Query Frame = 0

Query: 16  DGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLAFFGVGVNLVLFLTRVLQQSNVDAAN 75
           DG++D +G P  + K+G+W A   IL N+    LA++G+  NL+ + T  L +SNV AA+
Sbjct: 38  DGSIDIYGNPPSKKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELHESNVSAAS 97

Query: 76  NVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSLSSYLFLIRPNGC-G 135
           +V  W GT YI  L+GA ++DSYWGRY T A F  I+ IG+  L+LS+ L +++P  C G
Sbjct: 98  DVMIWQGTCYITPLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPVLKPAACAG 157

Query: 136 DEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEEDQKEGHYKVAFFSY 195
                C   +  + ++F+  +YL ALG GG +P +++FGADQFD+ D +E   K +FF++
Sbjct: 158 VAAALCSPATTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDPRERVRKASFFNW 217

Query: 196 FYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSAIAALLLFLAGTPRYRHFKPSGNPL 255
           FY ++N+GS  S+T+L + ++   W LGF +       ++  F  GTP YR  KP G+P+
Sbjct: 218 FYFSINIGSFISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTPLYRFQKPGGSPI 277

Query: 256 TRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNNGCRKILHTNGFK---------DSS 315
           TRVCQV+V+A +K ++ +P     L+E  EK +   G RKI HT+G+K         +  
Sbjct: 278 TRVCQVLVAAYRKLKLNLPEDISFLYETREKNSMIAGSRKIQHTDGYKFLDKAAVISEYE 337

Query: 316 DKEQGMINPWRLCPITQVEEVKCILKLMPIWLCTIIYSVVFTQMASLFVEQGAAMKTTIS 375
            K     NPW+LC +TQVEEVK ++++ PIW   I+YSV+++Q+++LFV+QG +M   I 
Sbjct: 338 SKSGAFSNPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTLFVQQGRSMNRIIR 397

Query: 376 NFHIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLTELQRMGVGLIIAVMAM 435
           +F IPPAS   FD L V + I +Y R L PFV +      KGLT+LQRMG+GL ++V+++
Sbjct: 398 SFEIPPASFGVFDTLIVLISIPIYDRFLVPFVRRF-TGIPKGLTDLQRMGIGLFLSVLSI 457

Query: 436 VSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVPQYALIGASEVFMYVGQLEFFNAQAPD 495
            +A IVE  RL+ AQ          ++SIFWQ+PQY L+G +EVF ++G++EFF  ++PD
Sbjct: 458 AAAAIVETVRLQLAQ-------DFVAMSIFWQIPQYILMGIAEVFFFIGRVEFFYDESPD 517

Query: 496 GLKSFGSALCMTSISLGNYVSSLLVTMVMKISTEDDMPGWIPGNLNKGHLDRFYFLLAAL 555
            ++S  SAL + + ++G+Y+SSL++T+V   +      GW+P +LNKGHLD F++LL +L
Sbjct: 518 AMRSVCSALALLNTAVGSYLSSLILTLVAYFTALGGKDGWVPDDLNKGHLDYFFWLLVSL 577

Query: 556 TVIDFVIY-VACAKWYK 562
            +++  +Y + C K  K
Sbjct: 578 GLVNIPVYALICVKHTK 586

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LQL22.5e-26177.12Protein NRT1/ PTR FAMILY 7.3 OS=Arabidopsis thaliana OX=3702 GN=NPF7.3 PE=1 SV=2[more]
Q8GXN23.8e-22266.84Protein NRT1/ PTR FAMILY 7.2 OS=Arabidopsis thaliana OX=3702 GN=NPF7.2 PE=2 SV=2[more]
Q3E9B51.8e-17958.87Protein NRT1/ PTR FAMILY 7.1 OS=Arabidopsis thaliana OX=3702 GN=NPF7.1 PE=2 SV=1[more]
Q9M3902.0e-13342.93Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Q93Z204.7e-12741.65Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023553282.10.098.14protein NRT1/ PTR FAMILY 7.3-like [Cucurbita pepo subsp. pepo][more]
XP_022923413.10.097.80protein NRT1/ PTR FAMILY 7.3-like [Cucurbita moschata][more]
KAG6577630.10.097.63Protein NRT1/ PTR FAMILY 7.3, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_023007750.10.097.46protein NRT1/ PTR FAMILY 7.3-like [Cucurbita maxima][more]
XP_038906251.10.092.02protein NRT1/ PTR FAMILY 7.3-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1E9L00.097.80protein NRT1/ PTR FAMILY 7.3-like OS=Cucurbita moschata OX=3662 GN=LOC111431116 ... [more]
A0A6J1L3U90.097.46protein NRT1/ PTR FAMILY 7.3-like OS=Cucurbita maxima OX=3661 GN=LOC111500269 PE... [more]
A0A1S3BKC80.091.34protein NRT1/ PTR FAMILY 7.3-like OS=Cucumis melo OX=3656 GN=LOC103490806 PE=3 S... [more]
A0A6J1DMR40.090.83protein NRT1/ PTR FAMILY 7.3-like OS=Momordica charantia OX=3673 GN=LOC111022668... [more]
A0A0A0L1B90.090.32Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G015860 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G32450.11.7e-26277.12nitrate transporter 1.5 [more]
AT4G21680.12.7e-22366.84NITRATE TRANSPORTER 1.8 [more]
AT5G19640.11.3e-18058.87Major facilitator superfamily protein [more]
AT3G54140.11.4e-13442.93peptide transporter 1 [more]
AT1G62200.13.3e-12841.65Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 102..519
e-value: 1.3E-93
score: 313.9
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 11..570
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 7..568
e-value: 8.1E-179
score: 597.2
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 25..568
NoneNo IPR availablePANTHERPTHR11654:SF546BNAA05G35790D PROTEINcoord: 11..570
NoneNo IPR availableCDDcd17419MFS_NPF7coord: 34..559
e-value: 0.0
score: 696.045

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG14g08930.1Cp4.1LG14g08930.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098656 anion transmembrane transport
biological_process GO:0010150 leaf senescence
biological_process GO:0015706 nitrate transport
biological_process GO:0055075 potassium ion homeostasis
biological_process GO:0071805 potassium ion transmembrane transport
biological_process GO:1902600 proton transmembrane transport
biological_process GO:0010167 response to nitrate
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015112 nitrate transmembrane transporter activity
molecular_function GO:0015386 potassium:proton antiporter activity
molecular_function GO:0022857 transmembrane transporter activity