Cp4.1LG14g07490 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG14g07490
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionPectin lyase-like superfamily protein
LocationCp4.1LG14: 5770295 .. 5771317 (+)
RNA-Seq ExpressionCp4.1LG14g07490
SyntenyCp4.1LG14g07490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGTCGACAACTTTTTTGTCGACGGTTATGGCAGCTTGAACGGCCGCGGTTCTTCAGCTTGGTCTATCAAAACTTTTAACCGTCCGATTGTAAATCCTTTTCTCGATTTTTTGGTTAAAGTGTTAGATGGAATAGGAATTCACTTTTTTTTCTTTTTTTAAAGTTATTTGTTTAGTGACCGTGAAAGTGTTTATATGTAGTCTATGAAACTCAATAAAATCAACCATGCGAGAATTACGGGTATAACATCGTACAATAGTAAATACTTCCATTTTGGTGTGCATGGATGTAGTGATGTCACGTTTGATCACATTAGAGTAATGGCTCTAGAAGATAGCCCCAACACAGATGGAATCCACATTTCCAAGTCAAGTGGTATCAACATTGTGCATTCAAGATTCGAAACGGGAGACGATTGCATCTCTCTTGGTCCTGGAAGTAAATTCATCAAAATAACAGACGTCCAGTGTGGTCCAGGTCATGGAGTTAGCATCGATAGCCTCGGAAAGTACCCAAATGAAGAAGATGTCTCTGAAGTCATAGTAAGAGACTCCACATTTGTTGGCACAAACAACGGTGTGAGGATTAAAATATGGTCATCTTCACTTCCTAGTTTTGTTACCAAGATCGCTTTTCTCAATCTCGAAATGAAGGACGTAAAAAGTCCTATTGTCATTGATAAAAAATATTGCCTTGATTGCAACAGAAATAAAATGGTATATATATTCAAATTTGATAGTCTTTGGATAAAGTGAAATTGAAAATGGTATTAATGAATGTTGTTTGTGGTCGACAGAGTAACTCAAAAGTGAAGATTCGAGACGGAGATATGAAAATATACAGGGGATTTCAACGACCCAAGTATGGATAAATTTGGAATGCAGCCAAGTTTTACCATGCGAAGGCATAGTGTTGGGTAACATTAACCTTCCCTTCAAGGGTGCTCGCCAACCAACTTCCATTTGTAATAATGTGAAGGGCCATGCTTTTGGCAAACAATTTCCAAACTCTTGCCTTTAA

mRNA sequence

ATGAACTTGAACGGCCGCGGTTCTTCAGCTTGGTCTATCAAAACTTTTAACCGTCCGATTGTAAATCCTTTTCTCGATTTTTTGGTTAAAGTTTATTTGTTTAGTGACCGTGAAAGTTCTATGAAACTCAATAAAATCAACCATGCGAGAATTACGGGTATAACATCGTACAATAGTAAATACTTCCATTTTGGTGTGCATGGATGTAGTGATGTCACGTTTGATCACATTAGAGTAATGGCTCTAGAAGATAGCCCCAACACAGATGGAATCCACATTTCCAAGTCAAGTGGTATCAACATTGTGCATTCAAGATTCGAAACGGGAGACGATTGCATCTCTCTTGGTCCTGGAAGTAAATTCATCAAAATAACAGACGTCCAGTGTGGTCCAGGTCATGGAGTTAGCATCGATAGCCTCGGAAAGTACCCAAATGAAGAAGATGTCTCTGAAGTCATAGTAAGAGACTCCACATTTGTTGGCACAAACAACGGTGTGAGGATTAAAATATGGTCATCTTCACTTCCTAGTTTTGTTACCAAGATCGCTTTTCTCAATCTCGAAATGAAGGACGTAAAAAGTCCTATTGTCATTGATAAAAAATATTGCCTTGATTGCAACAGAAATAAAATGAGTAACTCAAAAGTGAAGATTCGAGACGGAGATATGAAAATATACAGGGGATTTCAACGACCCAACCAAGTTTTACCATGCGAAGGCATAGTGTTGGGTAACATTAACCTTCCCTTCAAGGGTGCTCGCCAACCAACTTCCATTTGTAATAATGTGAAGGGCCATGCTTTTGGCAAACAATTTCCAAACTCTTGCCTTTAA

Coding sequence (CDS)

ATGAACTTGAACGGCCGCGGTTCTTCAGCTTGGTCTATCAAAACTTTTAACCGTCCGATTGTAAATCCTTTTCTCGATTTTTTGGTTAAAGTTTATTTGTTTAGTGACCGTGAAAGTTCTATGAAACTCAATAAAATCAACCATGCGAGAATTACGGGTATAACATCGTACAATAGTAAATACTTCCATTTTGGTGTGCATGGATGTAGTGATGTCACGTTTGATCACATTAGAGTAATGGCTCTAGAAGATAGCCCCAACACAGATGGAATCCACATTTCCAAGTCAAGTGGTATCAACATTGTGCATTCAAGATTCGAAACGGGAGACGATTGCATCTCTCTTGGTCCTGGAAGTAAATTCATCAAAATAACAGACGTCCAGTGTGGTCCAGGTCATGGAGTTAGCATCGATAGCCTCGGAAAGTACCCAAATGAAGAAGATGTCTCTGAAGTCATAGTAAGAGACTCCACATTTGTTGGCACAAACAACGGTGTGAGGATTAAAATATGGTCATCTTCACTTCCTAGTTTTGTTACCAAGATCGCTTTTCTCAATCTCGAAATGAAGGACGTAAAAAGTCCTATTGTCATTGATAAAAAATATTGCCTTGATTGCAACAGAAATAAAATGAGTAACTCAAAAGTGAAGATTCGAGACGGAGATATGAAAATATACAGGGGATTTCAACGACCCAACCAAGTTTTACCATGCGAAGGCATAGTGTTGGGTAACATTAACCTTCCCTTCAAGGGTGCTCGCCAACCAACTTCCATTTGTAATAATGTGAAGGGCCATGCTTTTGGCAAACAATTTCCAAACTCTTGCCTTTAA

Protein sequence

MNLNGRGSSAWSIKTFNRPIVNPFLDFLVKVYLFSDRESSMKLNKINHARITGITSYNSKYFHFGVHGCSDVTFDHIRVMALEDSPNTDGIHISKSSGINIVHSRFETGDDCISLGPGSKFIKITDVQCGPGHGVSIDSLGKYPNEEDVSEVIVRDSTFVGTNNGVRIKIWSSSLPSFVTKIAFLNLEMKDVKSPIVIDKKYCLDCNRNKMSNSKVKIRDGDMKIYRGFQRPNQVLPCEGIVLGNINLPFKGARQPTSICNNVKGHAFGKQFPNSCL
Homology
BLAST of Cp4.1LG14g07490 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 2.5e-54
Identity = 109/280 (38.93%), Postives = 164/280 (58.57%), Query Frame = 0

Query: 3   LNGRGSSAWSIKTFNRPIVNPFLDFLVKVYLFSDRESSMKLNKINHARITGITSYNSKYF 62
           L+G+G +AW+    ++   NP                +++ + + HA +  ITS NSK F
Sbjct: 105 LDGQGQTAWAKNNCDK---NPNC---------KHAAMNLRFDFLKHAMVRDITSLNSKMF 164

Query: 63  HFGVHGCSDVTFDHIRVMALEDSPNTDGIHISKSSGINIVHSRFETGDDCISLGPGSKFI 122
           H  V  C D+TF H+ V A   S NTDGIH+  S G+ I +++  TGDDCIS+GPGS+ +
Sbjct: 165 HINVLECEDITFQHVTVTAPGTSINTDGIHVGISKGVTITNTKIATGDDCISIGPGSQNV 224

Query: 123 KITDVQCGPGHGVSIDSLGKYPNEEDVSEVIVRDSTFVGTNNGVRIKIWSSSLPSFVTKI 182
            IT V CGPGHG+SI SLG+Y NE++V  + V+  TF GT NGVR+K W +S P   T +
Sbjct: 225 TITQVNCGPGHGISIGSLGRYNNEKEVRGITVKGCTFSGTMNGVRVKTWPNSPPGAATDL 284

Query: 183 AFLNLEMKDVKSPIVIDKKYCLDCNRNKMSNSKVKIRDGDMKIYRGFQRP--------NQ 242
            F +L M +V++P+++D++YC     ++ + S++K+ + +    RG            + 
Sbjct: 285 TFQDLTMNNVQNPVILDQEYCPYGQCSRQAPSRIKLSNINFNNIRGTSTGKVAVVIACSH 344

Query: 243 VLPCEGIVLGNINLPFKGARQP-TSICNNVKGHAFGKQFP 274
            +PC  + +G INL ++GA  P TS C+NVK    GKQ P
Sbjct: 345 GMPCSNMKIGEINLSYRGAGGPATSTCSNVKPTFSGKQVP 372

BLAST of Cp4.1LG14g07490 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 2.2e-50
Identity = 99/246 (40.24%), Postives = 147/246 (59.76%), Query Frame = 0

Query: 40  SMKLNKINHARITGITSYNSKYFHFGVHGCSDVTFDHIRVMALEDSPNTDGIHISKSSGI 99
           +++L  + ++ +  +T+ +SK FH  V GC ++TF+  ++ A E S NTDGIHI +S G+
Sbjct: 109 NLRLYAVTNSILRDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGV 168

Query: 100 NIVHSRFETGDDCISLGPGSKFIKITDVQCGPGHGVSIDSLGKYPNEEDVSEVIVRDSTF 159
           NI+++  +TGDDCISLG GSK I IT++ CGPGHG+S+ SLG+Y NEE V  + V++ T 
Sbjct: 169 NIINTEIKTGDDCISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTI 228

Query: 160 VGTNNGVRIKIWSSSLPSFVTKIAFLNLEMKDVKSPIVIDKKYCLDCNRNKMSNSKVKIR 219
            G+ NGVRIK W  S P   +++ F ++ M  V +PI+ID+ YC          S VK+ 
Sbjct: 229 TGSQNGVRIKTWPKSEPGEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLS 288

Query: 220 DGDMKIYRGFQRPNQVL--------PCEGIVLGNINLPFKGARQP-TSICNNVKGHAFGK 277
               K  +G     + +        PC G+ L +I+L + G   P TS+C N+K    GK
Sbjct: 289 KISFKNIKGTSTTKEAVKLVCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTIKGK 348

BLAST of Cp4.1LG14g07490 vs. ExPASy Swiss-Prot
Match: Q05967 (Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 2.2e-50
Identity = 109/283 (38.52%), Postives = 159/283 (56.18%), Query Frame = 0

Query: 3   LNGRGSSAWSIKTFNRPIVNPFLDFLVKVYLFSDRESSMKLNKINHARITGITSYNSKYF 62
           L+G+ ++AW  K   +    P               +++  N + ++ I  IT+ +SK F
Sbjct: 121 LDGQAAAAWECKQSKKCNKLP---------------NNLSFNSLTNSTIKDITTLDSKSF 180

Query: 63  HFGVHGCSDVTFDHIRVMALEDSPNTDGIHISKSSGINIVHSRFETGDDCISLGPGSKFI 122
           H  V+ C ++TF    V A  +SPNTDGIH+S+SS +NI  S F TGDDCIS+G  ++ +
Sbjct: 181 HVNVNQCKNLTFIRFNVSAPANSPNTDGIHVSRSSSVNITDSNFSTGDDCISVGDETEQL 240

Query: 123 KITDVQCGPGHGVSIDSLGKYPNEEDVSEVIVRDSTFVGTNNGVRIKIWSSSLPSFVTKI 182
            IT V CGPGHG+S+ SLG  P+E+ V  V VR+ TF  T+NGVRIK W +S P  V  +
Sbjct: 241 YITRVTCGPGHGISVGSLGGNPDEKPVVGVFVRNCTFTNTDNGVRIKTWPASHPGVVNDV 300

Query: 183 AFLNLEMKDVKSPIVIDKKYCLDCNRNKMSNSKVKIRDGDMKIYRGFQRPNQV------- 242
            F ++ +++V +P+VID+ YC     NK   S+VKI     +  +G  R           
Sbjct: 301 HFEDIIVQNVSNPVVIDQVYCPFNKCNKDLPSQVKISKVSFQNIKGTSRTQDAVSLLRSK 360

Query: 243 -LPCEGIVLGNINLPFKGARQPT-SICNNVKGHAFGKQFPNSC 277
            +PCEGI +G+I++ + G   P  S C N+K    GKQ P  C
Sbjct: 361 GVPCEGIEVGDIDITYSGKEGPAKSSCENIKPSLKGKQNPPVC 388

BLAST of Cp4.1LG14g07490 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 7.1e-49
Identity = 98/245 (40.00%), Postives = 145/245 (59.18%), Query Frame = 0

Query: 40  SMKLNKINHARITGITSYNSKYFHFGVHGCSDVTFDHIRVMALEDSPNTDGIHISKSSGI 99
           +++ + + +A I  ITS +SK FH  V  C ++T + +++ A ++SPNTDGIH+ KS G+
Sbjct: 151 NIRFDFVTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEGV 210

Query: 100 NIVHSRFETGDDCISLGPGSKFIKITDVQCGPGHGVSIDSLGKYPNEEDVSEVIVRDSTF 159
           NI+ S  +TGDDCIS+G G+K + I ++ CGPGHG+SI SLGK+ NEE V  + + + T 
Sbjct: 211 NIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCTI 270

Query: 160 VGTNNGVRIKIWSSSLPSFVTKIAFLNLEMKDVKSPIVIDKKYCLDCNRNKMSNSKVKIR 219
             T+NG RIK W       V++I F ++ M +V SPI+ID++YC      K   SKVK+ 
Sbjct: 271 TNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKLS 330

Query: 220 DGDMKIYRGFQRPNQVL--------PCEGIVLGNINLPFKGARQPTSICNNVKGHAFGKQ 277
           +   K  RG     + +        PC+ + L +I++   GA   TS C NVK    GK 
Sbjct: 331 NISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDIQHNGAEPATSQCLNVKPITIGKL 390

BLAST of Cp4.1LG14g07490 vs. ExPASy Swiss-Prot
Match: P35339 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.6e-48
Identity = 104/277 (37.55%), Postives = 156/277 (56.32%), Query Frame = 0

Query: 3   LNGRGSSAWSIKTFNRPIVNPFLDFLVKVYLFSDRESSMKLNKINHARITGITSYNSKYF 62
           L+G+G + WS  +             VK Y      +S+ ++ +N+  ++GIT  NSK+F
Sbjct: 138 LDGQGPAVWSKNS------------CVKKYDCKILPNSLVMDFVNNGEVSGITLLNSKFF 197

Query: 63  HFGVHGCSDVTFDHIRVMALEDSPNTDGIHISKSSGINIVHSRFETGDDCISLGPGSKFI 122
           H  ++ C D+    + V A  DSPNTDGIH+  SSG+ I ++    GDDCIS+GPG+  +
Sbjct: 198 HMNMYKCKDMLIKDVNVTAPGDSPNTDGIHMGDSSGVTITNTVIGVGDDCISIGPGTSKV 257

Query: 123 KITDVQCGPGHGVSIDSLGKYPNEEDVSEVIVRDSTFVGTNNGVRIKIWSSSLPSF-VTK 182
            IT V CGPGHG+SI SLG+Y +E+DV+++ V+D T   T NGVRIK +  +      +K
Sbjct: 258 NITGVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTLKKTANGVRIKAYEDAASVLTASK 317

Query: 183 IAFLNLEMKDVKSPIVIDKKYCLDCNRNKMSNSKVKIRDGDMKIYRG-FQRPNQV----- 242
           I + N++M+D   PI+ID KYC +        SKV ++D   K   G    P  V     
Sbjct: 318 IHYENIKMEDSGYPIIIDMKYCPNKLCTANGASKVTVKDVTFKNITGTSSTPEAVNLLCT 377

Query: 243 --LPCEGIVLGNINLPFKGARQPT-SICNNVKGHAFG 270
             +PC G+ + ++N+ + G    T ++C N KG A G
Sbjct: 378 AKIPCTGVTMDDVNIKYSGTNNKTMAVCKNAKGSAKG 402

BLAST of Cp4.1LG14g07490 vs. NCBI nr
Match: XP_023552930.1 (exopolygalacturonase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 422 bits (1086), Expect = 3.20e-145
Identity = 212/283 (74.91%), Postives = 230/283 (81.27%), Query Frame = 0

Query: 3   LNGRGSSAWSIKTFNRPIVNPFLDFLVKVYLFSDRESSMKLNKINHARITGITSYNSKYF 62
           LNGRGS AWSIK FNRPI                   SMKL+KINHARITGITSYNSK F
Sbjct: 125 LNGRGSLAWSIKKFNRPI-------------------SMKLSKINHARITGITSYNSKNF 184

Query: 63  HFGVHGCSDVTFDHIRVMALEDSPNTDGIHISKSSGINIVHSRFETGDDCISLGPGSKFI 122
           HF VHGC+DVTFDHIR MA +DSPNTDGIHISKSSGINI+HSRF+TGDDCISLGPGS+FI
Sbjct: 185 HFSVHGCNDVTFDHIRTMAPKDSPNTDGIHISKSSGINIMHSRFDTGDDCISLGPGSRFI 244

Query: 123 KITDVQCGPGHGVSIDSLGKYPNEEDVSEVIVRDSTFVGTNNGVRIKIWSSSLPSFVTKI 182
            IT++ CGPGHG+SI SLGKYPNEEDVS+VIVRDSTFVGT+NGVRIK WSSSLPS VTKI
Sbjct: 245 NITNIYCGPGHGISIGSLGKYPNEEDVSDVIVRDSTFVGTDNGVRIKTWSSSLPSSVTKI 304

Query: 183 AFLNLEMKDVKSPIVIDKKYCLDCNRNKMSNSKVKIRDGDMKIYRGFQRP--------NQ 242
            FLNLEMKDVK+PIVID+ YCLDC R++MSNS+VKIRD   +  RG            + 
Sbjct: 305 TFLNLEMKDVKNPIVIDQNYCLDCQRDQMSNSRVKIRDVRYENIRGTSATQVAVNFECSH 364

Query: 243 VLPCEGIVLGNINLPFKGARQPTSICNNVKGHAFGKQFPNSCL 277
           VLPCE IVLGNINLPFKGA QPTSICNNVKGHAFGKQFPNSCL
Sbjct: 365 VLPCEDIVLGNINLPFKGAGQPTSICNNVKGHAFGKQFPNSCL 388

BLAST of Cp4.1LG14g07490 vs. NCBI nr
Match: XP_023007598.1 (exopolygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 405 bits (1041), Expect = 3.91e-138
Identity = 202/283 (71.38%), Postives = 226/283 (79.86%), Query Frame = 0

Query: 3   LNGRGSSAWSIKTFNRPIVNPFLDFLVKVYLFSDRESSMKLNKINHARITGITSYNSKYF 62
            +GRG  AWSIKTFNRPI                   SMKL KINHARITGITSYNSK+F
Sbjct: 142 FDGRGFLAWSIKTFNRPI-------------------SMKLTKINHARITGITSYNSKFF 201

Query: 63  HFGVHGCSDVTFDHIRVMALEDSPNTDGIHISKSSGINIVHSRFETGDDCISLGPGSKFI 122
           HFGVHGCSDVTFDHIRVMA  DSPNTDGIHISKSSGINIVHSRF+TGDDCISLGPGS+FI
Sbjct: 202 HFGVHGCSDVTFDHIRVMAPRDSPNTDGIHISKSSGINIVHSRFDTGDDCISLGPGSRFI 261

Query: 123 KITDVQCGPGHGVSIDSLGKYPNEEDVSEVIVRDSTFVGTNNGVRIKIWSSSLPSFVTKI 182
            IT++ CGPGHG+SI SLGKYPNEEDVS+V+VRDSTFVGT+NGVRIK WSSS PSFVTKI
Sbjct: 262 NITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTFVGTDNGVRIKTWSSSHPSFVTKI 321

Query: 183 AFLNLEMKDVKSPIVIDKKYCLDCNRNKMSNSKVKIRDGDMKIYRGFQRP--------NQ 242
            FLNLEMKDV++P++ID+KYCLDC+R+KMS S+V+IRD   +  RG            +Q
Sbjct: 322 VFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQIRDVRYEGIRGTSATQAAVNFECSQ 381

Query: 243 VLPCEGIVLGNINLPFKGARQPTSICNNVKGHAFGKQFPNSCL 277
           V PCEGIVL +INL F G  QPTSIC+NVKG AFGKQ P+SCL
Sbjct: 382 VFPCEGIVLQDINLSFNGGGQPTSICHNVKGSAFGKQLPSSCL 405

BLAST of Cp4.1LG14g07490 vs. NCBI nr
Match: XP_023552929.1 (exopolygalacturonase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 406 bits (1043), Expect = 1.85e-136
Identity = 204/283 (72.08%), Postives = 227/283 (80.21%), Query Frame = 0

Query: 3   LNGRGSSAWSIKTFNRPIVNPFLDFLVKVYLFSDRESSMKLNKINHARITGITSYNSKYF 62
           LNGRGS AWSIK FNRPI                   SMKL+KINHARIT ITSYNSK+F
Sbjct: 282 LNGRGSLAWSIKKFNRPI-------------------SMKLSKINHARITSITSYNSKFF 341

Query: 63  HFGVHGCSDVTFDHIRVMALEDSPNTDGIHISKSSGINIVHSRFETGDDCISLGPGSKFI 122
           HFGVHGC+DVTFDHIR MA +DSPNTDGIHISKSSGINI+HSRF+TGDDCISLGPGS+FI
Sbjct: 342 HFGVHGCNDVTFDHIRTMAPKDSPNTDGIHISKSSGINIMHSRFDTGDDCISLGPGSRFI 401

Query: 123 KITDVQCGPGHGVSIDSLGKYPNEEDVSEVIVRDSTFVGTNNGVRIKIWSSSLPSFVTKI 182
            IT++ CGPGHG+SI SLGK+PNEEDVS+V+VRDSTFVGT+NGVRIK WSSS PSFVTKI
Sbjct: 402 NITNIHCGPGHGISIGSLGKFPNEEDVSDVLVRDSTFVGTDNGVRIKTWSSSHPSFVTKI 461

Query: 183 AFLNLEMKDVKSPIVIDKKYCLDCNRNKMSNSKVKIRDGDMKIYRGFQRP--------NQ 242
           AFLNLEMKDVK+PIVID+KYCL CNRN+MSNS+VKI D   +  RG            +Q
Sbjct: 462 AFLNLEMKDVKNPIVIDQKYCLGCNRNEMSNSRVKIGDVRYENIRGTSATQAAVNFECSQ 521

Query: 243 VLPCEGIVLGNINLPFKGARQPTSICNNVKGHAFGKQFPNSCL 277
           VLPCEGIVL +INL F G  QPTSIC+NVKG A GKQ P+SCL
Sbjct: 522 VLPCEGIVLQDINLSFNGRGQPTSICHNVKGSALGKQLPSSCL 545

BLAST of Cp4.1LG14g07490 vs. NCBI nr
Match: XP_022932245.1 (exopolygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 377 bits (968), Expect = 1.62e-128
Identity = 191/226 (84.51%), Postives = 195/226 (86.28%), Query Frame = 0

Query: 3   LNGRGSSAWSIKTFNRPIVNPFLDFLVKVYLFSDRESSMKLNKINHARITGITSYNSKYF 62
           LNGRGSSAWSIKTFNRPI                   SMKLNKINHARITGITSYNSK F
Sbjct: 80  LNGRGSSAWSIKTFNRPI-------------------SMKLNKINHARITGITSYNSKCF 139

Query: 63  HFGVHGCSDVTFDHIRVMALEDSPNTDGIHISKSSGINIVHSRFETGDDCISLGPGSKFI 122
           HFGVHGCSDV FDHIRVMA EDSPNTDGIHISKSSGINIVHSRFETGDDCISLGPGSKFI
Sbjct: 140 HFGVHGCSDVMFDHIRVMAPEDSPNTDGIHISKSSGINIVHSRFETGDDCISLGPGSKFI 199

Query: 123 KITDVQCGPGHGVSIDSLGKYPNEEDVSEVIVRDSTFVGTNNGVRIKIWSSSLPSFVTKI 182
            ITD+QCGPGHGVSI SLGKYPNEEDVSEVIVRDSTFVGTNNGVRIK WSSSLPSFVT I
Sbjct: 200 NITDIQCGPGHGVSIGSLGKYPNEEDVSEVIVRDSTFVGTNNGVRIKTWSSSLPSFVTNI 259

Query: 183 AFLNLEMKDVKSPIVIDKKYCLDCNRNKMSNSKVKIRDGDMKIYRG 228
           AFLNLEMKDVKSPIVIDKKYCLDCNR+KMSNS+VKIRD   +  RG
Sbjct: 260 AFLNLEMKDVKSPIVIDKKYCLDCNRDKMSNSRVKIRDVRYENIRG 286

BLAST of Cp4.1LG14g07490 vs. NCBI nr
Match: XP_022932248.1 (exopolygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 368 bits (945), Expect = 6.73e-124
Identity = 187/283 (66.08%), Postives = 213/283 (75.27%), Query Frame = 0

Query: 3   LNGRGSSAWSIKTFNRPIVNPFLDFLVKVYLFSDRESSMKLNKINHARITGITSYNSKYF 62
           LNG GSSAW   T NRP+                   SMKL+ +N ARI+ ITS NSK+F
Sbjct: 119 LNGGGSSAWPSDTSNRPV-------------------SMKLSSVNTARISKITSSNSKFF 178

Query: 63  HFGVHGCSDVTFDHIRVMALEDSPNTDGIHISKSSGINIVHSRFETGDDCISLGPGSKFI 122
           HFGVHGC DVTFDH+ V+A  DSPNTDGIH+SKSSGINI+HS   TGDDCISLGPGSKFI
Sbjct: 179 HFGVHGCRDVTFDHVTVIAPADSPNTDGIHVSKSSGINIMHSVIRTGDDCISLGPGSKFI 238

Query: 123 KITDVQCGPGHGVSIDSLGKYPNEEDVSEVIVRDSTFVGTNNGVRIKIWSSSLPSFVTKI 182
            ITD+QCGPGHG+SI SLGKYPNEEDVSEV +RDSTFVGT+NGVRIK WSSS  SFV+KI
Sbjct: 239 NITDIQCGPGHGISIGSLGKYPNEEDVSEVTIRDSTFVGTDNGVRIKTWSSSFSSFVSKI 298

Query: 183 AFLNLEMKDVKSPIVIDKKYCLDCNRNKMSNSKVKIRDGDMKIYRGFQRP--------NQ 242
           AFLNLEMKDVK+PI+ID+KYC DC  +KMSNSKV+IRD   +  RG            +Q
Sbjct: 299 AFLNLEMKDVKNPIIIDQKYCPDCKEDKMSNSKVQIRDVRYEDIRGSSTSQVAVSFECSQ 358

Query: 243 VLPCEGIVLGNINLPFKGARQPTSICNNVKGHAFGKQFPNSCL 277
           V  CE IVL +INL +KG  QPTSIC+NV+G A GKQFP+SCL
Sbjct: 359 VCSCESIVLQDINLSYKGDGQPTSICHNVQGSASGKQFPSSCL 382

BLAST of Cp4.1LG14g07490 vs. ExPASy TrEMBL
Match: A0A6J1L832 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111500170 PE=3 SV=1)

HSP 1 Score: 405 bits (1041), Expect = 1.89e-138
Identity = 202/283 (71.38%), Postives = 226/283 (79.86%), Query Frame = 0

Query: 3   LNGRGSSAWSIKTFNRPIVNPFLDFLVKVYLFSDRESSMKLNKINHARITGITSYNSKYF 62
            +GRG  AWSIKTFNRPI                   SMKL KINHARITGITSYNSK+F
Sbjct: 142 FDGRGFLAWSIKTFNRPI-------------------SMKLTKINHARITGITSYNSKFF 201

Query: 63  HFGVHGCSDVTFDHIRVMALEDSPNTDGIHISKSSGINIVHSRFETGDDCISLGPGSKFI 122
           HFGVHGCSDVTFDHIRVMA  DSPNTDGIHISKSSGINIVHSRF+TGDDCISLGPGS+FI
Sbjct: 202 HFGVHGCSDVTFDHIRVMAPRDSPNTDGIHISKSSGINIVHSRFDTGDDCISLGPGSRFI 261

Query: 123 KITDVQCGPGHGVSIDSLGKYPNEEDVSEVIVRDSTFVGTNNGVRIKIWSSSLPSFVTKI 182
            IT++ CGPGHG+SI SLGKYPNEEDVS+V+VRDSTFVGT+NGVRIK WSSS PSFVTKI
Sbjct: 262 NITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTFVGTDNGVRIKTWSSSHPSFVTKI 321

Query: 183 AFLNLEMKDVKSPIVIDKKYCLDCNRNKMSNSKVKIRDGDMKIYRGFQRP--------NQ 242
            FLNLEMKDV++P++ID+KYCLDC+R+KMS S+V+IRD   +  RG            +Q
Sbjct: 322 VFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQIRDVRYEGIRGTSATQAAVNFECSQ 381

Query: 243 VLPCEGIVLGNINLPFKGARQPTSICNNVKGHAFGKQFPNSCL 277
           V PCEGIVL +INL F G  QPTSIC+NVKG AFGKQ P+SCL
Sbjct: 382 VFPCEGIVLQDINLSFNGGGQPTSICHNVKGSAFGKQLPSSCL 405

BLAST of Cp4.1LG14g07490 vs. ExPASy TrEMBL
Match: A0A6J1F1P4 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111438609 PE=3 SV=1)

HSP 1 Score: 377 bits (968), Expect = 7.86e-129
Identity = 191/226 (84.51%), Postives = 195/226 (86.28%), Query Frame = 0

Query: 3   LNGRGSSAWSIKTFNRPIVNPFLDFLVKVYLFSDRESSMKLNKINHARITGITSYNSKYF 62
           LNGRGSSAWSIKTFNRPI                   SMKLNKINHARITGITSYNSK F
Sbjct: 80  LNGRGSSAWSIKTFNRPI-------------------SMKLNKINHARITGITSYNSKCF 139

Query: 63  HFGVHGCSDVTFDHIRVMALEDSPNTDGIHISKSSGINIVHSRFETGDDCISLGPGSKFI 122
           HFGVHGCSDV FDHIRVMA EDSPNTDGIHISKSSGINIVHSRFETGDDCISLGPGSKFI
Sbjct: 140 HFGVHGCSDVMFDHIRVMAPEDSPNTDGIHISKSSGINIVHSRFETGDDCISLGPGSKFI 199

Query: 123 KITDVQCGPGHGVSIDSLGKYPNEEDVSEVIVRDSTFVGTNNGVRIKIWSSSLPSFVTKI 182
            ITD+QCGPGHGVSI SLGKYPNEEDVSEVIVRDSTFVGTNNGVRIK WSSSLPSFVT I
Sbjct: 200 NITDIQCGPGHGVSIGSLGKYPNEEDVSEVIVRDSTFVGTNNGVRIKTWSSSLPSFVTNI 259

Query: 183 AFLNLEMKDVKSPIVIDKKYCLDCNRNKMSNSKVKIRDGDMKIYRG 228
           AFLNLEMKDVKSPIVIDKKYCLDCNR+KMSNS+VKIRD   +  RG
Sbjct: 260 AFLNLEMKDVKSPIVIDKKYCLDCNRDKMSNSRVKIRDVRYENIRG 286

BLAST of Cp4.1LG14g07490 vs. ExPASy TrEMBL
Match: A0A6J1F153 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111438612 PE=3 SV=1)

HSP 1 Score: 368 bits (945), Expect = 3.26e-124
Identity = 187/283 (66.08%), Postives = 213/283 (75.27%), Query Frame = 0

Query: 3   LNGRGSSAWSIKTFNRPIVNPFLDFLVKVYLFSDRESSMKLNKINHARITGITSYNSKYF 62
           LNG GSSAW   T NRP+                   SMKL+ +N ARI+ ITS NSK+F
Sbjct: 119 LNGGGSSAWPSDTSNRPV-------------------SMKLSSVNTARISKITSSNSKFF 178

Query: 63  HFGVHGCSDVTFDHIRVMALEDSPNTDGIHISKSSGINIVHSRFETGDDCISLGPGSKFI 122
           HFGVHGC DVTFDH+ V+A  DSPNTDGIH+SKSSGINI+HS   TGDDCISLGPGSKFI
Sbjct: 179 HFGVHGCRDVTFDHVTVIAPADSPNTDGIHVSKSSGINIMHSVIRTGDDCISLGPGSKFI 238

Query: 123 KITDVQCGPGHGVSIDSLGKYPNEEDVSEVIVRDSTFVGTNNGVRIKIWSSSLPSFVTKI 182
            ITD+QCGPGHG+SI SLGKYPNEEDVSEV +RDSTFVGT+NGVRIK WSSS  SFV+KI
Sbjct: 239 NITDIQCGPGHGISIGSLGKYPNEEDVSEVTIRDSTFVGTDNGVRIKTWSSSFSSFVSKI 298

Query: 183 AFLNLEMKDVKSPIVIDKKYCLDCNRNKMSNSKVKIRDGDMKIYRGFQRP--------NQ 242
           AFLNLEMKDVK+PI+ID+KYC DC  +KMSNSKV+IRD   +  RG            +Q
Sbjct: 299 AFLNLEMKDVKNPIIIDQKYCPDCKEDKMSNSKVQIRDVRYEDIRGSSTSQVAVSFECSQ 358

Query: 243 VLPCEGIVLGNINLPFKGARQPTSICNNVKGHAFGKQFPNSCL 277
           V  CE IVL +INL +KG  QPTSIC+NV+G A GKQFP+SCL
Sbjct: 359 VCSCESIVLQDINLSYKGDGQPTSICHNVQGSASGKQFPSSCL 382

BLAST of Cp4.1LG14g07490 vs. ExPASy TrEMBL
Match: A0A6J1L5E9 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111500171 PE=3 SV=1)

HSP 1 Score: 346 bits (887), Expect = 3.03e-113
Identity = 172/239 (71.97%), Postives = 194/239 (81.17%), Query Frame = 0

Query: 3   LNGRGSSAWSIKTFNRPIVNPFLDFLVKVYLFSDRESSMKLNKINHARITGITSYNSKYF 62
            +GRG  AWSIKTFNRPI                   SMKL KINHARITGITSYNSK+F
Sbjct: 142 FDGRGFLAWSIKTFNRPI-------------------SMKLTKINHARITGITSYNSKFF 201

Query: 63  HFGVHGCSDVTFDHIRVMALEDSPNTDGIHISKSSGINIVHSRFETGDDCISLGPGSKFI 122
           HFGVHGCSDVTFDHIRVMA  DSPNTDGIHISKSSGINIVHSRF+TGDDCISLGPGS+FI
Sbjct: 202 HFGVHGCSDVTFDHIRVMAPRDSPNTDGIHISKSSGINIVHSRFDTGDDCISLGPGSRFI 261

Query: 123 KITDVQCGPGHGVSIDSLGKYPNEEDVSEVIVRDSTFVGTNNGVRIKIWSSSLPSFVTKI 182
            IT++ CGPGHG+SI SLGKYPNEEDVS+V+VRDSTFVGT+NGVRIK WSSS PSFVTKI
Sbjct: 262 NITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTFVGTDNGVRIKTWSSSHPSFVTKI 321

Query: 183 AFLNLEMKDVKSPIVIDKKYCLDCNRNKMSNSKVKIRDGDMKIYRGFQRP-NQVLPCEG 240
            FLNLEMKDV++P++ID+KYCLDC+R+KMS S+V+IRD     Y G + P +    C+G
Sbjct: 322 VFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQIRDVR---YEGIKYPWSHACACDG 358

BLAST of Cp4.1LG14g07490 vs. ExPASy TrEMBL
Match: A0A6J1EVU6 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111438610 PE=3 SV=1)

HSP 1 Score: 333 bits (853), Expect = 2.26e-111
Identity = 162/209 (77.51%), Postives = 179/209 (85.65%), Query Frame = 0

Query: 3   LNGRGSSAWSIKTFNRPIVNPFLDFLVKVYLFSDRESSMKLNKINHARITGITSYNSKYF 62
           LNGRGS AWSIK FNRPI                   S+KL +INHARITGITSYNSK F
Sbjct: 94  LNGRGSLAWSIKKFNRPI-------------------SVKLFRINHARITGITSYNSKNF 153

Query: 63  HFGVHGCSDVTFDHIRVMALEDSPNTDGIHISKSSGINIVHSRFETGDDCISLGPGSKFI 122
           HFGVHGC+DVTFDHIR MA +DSPNTDGIHISKSSGINI+HSRF+TGDDCISLGPGS+FI
Sbjct: 154 HFGVHGCNDVTFDHIRTMAPKDSPNTDGIHISKSSGINIMHSRFDTGDDCISLGPGSRFI 213

Query: 123 KITDVQCGPGHGVSIDSLGKYPNEEDVSEVIVRDSTFVGTNNGVRIKIWSSSLPSFVTKI 182
            IT++ CGPGHG+SI SLGKYPNEEDVS+V+VRDSTFVGT+NGVRIK WSSSLPS VTKI
Sbjct: 214 NITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTFVGTDNGVRIKTWSSSLPSSVTKI 273

Query: 183 AFLNLEMKDVKSPIVIDKKYCLDCNRNKM 211
           AF+NLEMKDVKSPI+ID+KYCLDCNR+KM
Sbjct: 274 AFINLEMKDVKSPIIIDQKYCLDCNRDKM 283

BLAST of Cp4.1LG14g07490 vs. TAIR 10
Match: AT3G07840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 203.0 bits (515), Expect = 3.2e-52
Identity = 105/283 (37.10%), Postives = 166/283 (58.66%), Query Frame = 0

Query: 4   NGRGSSAWSIKTFNRPIVNPFLDFLVKVYLFSDRESSMKLNKINHARITGITSYNSKYFH 63
           +G G++AW +   +            K +       S++ + + +A I  I+S ++K FH
Sbjct: 117 DGEGNAAWRVNNCH------------KTFECKKLPISIRFDFVENAEIRDISSIDAKNFH 176

Query: 64  FGVHGCSDVTFDHIRVMALEDSPNTDGIHISKSSGINIVHSRFETGDDCISLGPGSKFIK 123
             V G  ++TFD+++V+A  +SPNTDGIH+ +S G+ I++S+  TGDDCIS+G G K + 
Sbjct: 177 INVLGAKNMTFDNVKVIAPAESPNTDGIHLGRSEGVKILNSKIATGDDCISVGDGMKNLH 236

Query: 124 ITDVQCGPGHGVSIDSLGKYPNEEDVSEVIVRDSTFVGTNNGVRIKIW-SSSLPSFVTKI 183
           + +V CGPGHG+S+ SLG+Y +E+DV+ + V + T  GT+NG+RIK W S++  +  + I
Sbjct: 237 VENVMCGPGHGISVGSLGRYVHEQDVTGITVVNCTLQGTDNGLRIKTWPSAACATTASGI 296

Query: 184 AFLNLEMKDVKSPIVIDKKYCLDCNRNKMSNSKVKIRDGDMKIYRGFQRPNQVL------ 243
            F N+ + +V +PI+ID++YC     NK   S +K+ D   K  RG       +      
Sbjct: 297 HFENIILNNVSNPILIDQEYCPWNQCNKQKPSTIKLVDISFKNIRGTSGNKDAVKLLCSK 356

Query: 244 --PCEGIVLGNINLPFKGA-RQPTSICNNVKGHAFGKQFPNSC 277
             PC  + +GNINL +KGA   PT +C+NV     G Q P +C
Sbjct: 357 AHPCANVEIGNINLEYKGADGPPTFMCSNVSPKLVGTQNPKAC 387

BLAST of Cp4.1LG14g07490 vs. TAIR 10
Match: AT3G07830.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 200.7 bits (509), Expect = 1.6e-51
Identity = 101/283 (35.69%), Postives = 169/283 (59.72%), Query Frame = 0

Query: 4   NGRGSSAWSIKTFNRPIVNPFLDFLVKVYLFSDRESSMKLNKINHARITGITSYNSKYFH 63
           +G G++AW +   +            K +       S++ + + +A I  I+S ++K FH
Sbjct: 117 DGEGNAAWRVNNCH------------KTFECKKLPISIRFDFVTNAEIRDISSIDAKNFH 176

Query: 64  FGVHGCSDVTFDHIRVMALEDSPNTDGIHISKSSGINIVHSRFETGDDCISLGPGSKFIK 123
             V G  ++TFD++++MA  +SPNTDGIH+ +S G++I++SR  TGDDC+S+G G   + 
Sbjct: 177 INVIGAKNMTFDNVKIMAPAESPNTDGIHLGRSVGVSIINSRISTGDDCVSVGDGMVNLL 236

Query: 124 ITDVQCGPGHGVSIDSLGKYPNEEDVSEVIVRDSTFVGTNNGVRIKIW-SSSLPSFVTKI 183
           + +V CGPGHG+S+ SLG+Y +E+DVS + V + T   T+NG+RIK W S++  +  + I
Sbjct: 237 VKNVVCGPGHGISVGSLGRYGHEQDVSGIRVINCTLQETDNGLRIKTWPSAACSTTASNI 296

Query: 184 AFLNLEMKDVKSPIVIDKKYCLDCNRNKMSNSKVKIRDGDMKIYRGFQRPNQVL------ 243
            F N+ +++V +PI+ID++YC     NK  +S +K+ +   +  RG       +      
Sbjct: 297 HFENIILRNVSNPILIDQEYCPWNQCNKQKSSSIKLANISFRRIRGTSGNKDAVKLLCSK 356

Query: 244 --PCEGIVLGNINLPFKGARQP-TSICNNVKGHAFGKQFPNSC 277
             PCE + +G+IN+ + GA  P T +C+NV+    G QFP +C
Sbjct: 357 GYPCENVQVGDINIQYTGADGPATFMCSNVRPKLVGTQFPKAC 387

BLAST of Cp4.1LG14g07490 vs. TAIR 10
Match: AT5G48140.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 198.0 bits (502), Expect = 1.0e-50
Identity = 101/283 (35.69%), Postives = 164/283 (57.95%), Query Frame = 0

Query: 4   NGRGSSAWSIKTFNRPIVNPFLDFLVKVYLFSDRESSMKLNKINHARITGITSYNSKYFH 63
           +G G++AW +   +            K +       S++ + +  A+I GITS ++K+FH
Sbjct: 116 DGEGNAAWRVNNCH------------KTFNCKKLPISIRFDFVTDAKIRGITSLDAKHFH 175

Query: 64  FGVHGCSDVTFDHIRVMALEDSPNTDGIHISKSSGINIVHSRFETGDDCISLGPGSKFIK 123
             V G  +VTF+ ++++A  +SPNTDGIH+ +S GI I++S   TGDDC+S+G G K + 
Sbjct: 176 INVIGAKNVTFEDVKIIAPAESPNTDGIHVGRSDGIKIINSFISTGDDCVSVGDGMKNLL 235

Query: 124 ITDVQCGPGHGVSIDSLGKYPNEEDVSEVIVRDSTFVGTNNGVRIKIW-SSSLPSFVTKI 183
           +  V CGPGHG+SI SLG+Y +EE+VS + + + T   T+NG+RIK W S++  +  + I
Sbjct: 236 VERVTCGPGHGISIGSLGRYSHEENVSGIKIINCTLQETDNGLRIKTWPSAACTTTASDI 295

Query: 184 AFLNLEMKDVKSPIVIDKKYCLDCNRNKMSNSKVKIRDGDMKIYRGFQRPNQVL------ 243
            F N+ +K+V +PI+ID++YC     NK   S +K+ +   K  RG       +      
Sbjct: 296 HFENILLKNVSNPILIDQEYCPWNQCNKQKPSTIKLANISFKKIRGTSGNKDAVKLLCSK 355

Query: 244 --PCEGIVLGNINLPFKGARQPTSI-CNNVKGHAFGKQFPNSC 277
             PC+ + +G++N+ + GA  P +  C+NV     G Q P +C
Sbjct: 356 GYPCQNVEVGDVNIQYTGADGPATFQCSNVSPKLVGTQIPKAC 386

BLAST of Cp4.1LG14g07490 vs. TAIR 10
Match: AT2G43890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 194.5 bits (493), Expect = 1.1e-49
Identity = 97/246 (39.43%), Postives = 142/246 (57.72%), Query Frame = 0

Query: 40  SMKLNKINHARITGITSYNSKYFHFGVHGCSDVTFDHIRVMALEDSPNTDGIHISKSSGI 99
           SM  N  N   ++G+TS NS+  H  ++ C++V    ++++A + SPNTDG+H+  S+G+
Sbjct: 148 SMTFNWANDVVVSGLTSINSQTTHLVINSCNNVIVRKVKLVAPDQSPNTDGLHVQGSAGV 207

Query: 100 NIVHSRFETGDDCISLGPGSKFIKITDVQCGPGHGVSIDSLGKYPNEEDVSEVIVRDSTF 159
            +    F TGDDCIS+GPG++ + ++ + CGPGHG+SI SLG+  NE  V  + + +S F
Sbjct: 208 TVTDGTFHTGDDCISIGPGTRNLYMSKLNCGPGHGISIGSLGRDANEAGVENITLINSVF 267

Query: 160 VGTNNGVRIKIWSSSLPSFVTKIAFLNLEMKDVKSPIVIDKKYCLDCNRNKMSNSKVKIR 219
            G++NGVRIK W+     FV  + F NL MK+V++PI++D+ YC          S VKI 
Sbjct: 268 SGSDNGVRIKTWARQSTGFVRNVLFQNLIMKNVQNPIIVDQNYCPSNQGCPKQGSGVKIS 327

Query: 220 DGDMKIYRGFQRPNQVL--------PCEGIVLGNINLPFKGARQPTSICNNVKGHAFGKQ 278
               +  +G  R  Q L        PC+ I L +I L F G R  TS C N+KG   G  
Sbjct: 328 QVVYRNIQGTSRTQQALTFDCSRSNPCQAIRLHDIKLTFNG-RSATSTCKNIKGVKAGVV 387

BLAST of Cp4.1LG14g07490 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 190.3 bits (482), Expect = 2.1e-48
Identity = 95/246 (38.62%), Postives = 146/246 (59.35%), Query Frame = 0

Query: 40  SMKLNKINHARITGITSYNSKYFHFGVHGCSDVTFDHIRVMALEDSPNTDGIHISKSSGI 99
           +++   + +++I  ITS NSK FH  +  C ++T   I + A  +S NTDGIHI +S+G+
Sbjct: 192 NIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLTDIGIDAPPESLNTDGIHIGRSNGV 251

Query: 100 NIVHSRFETGDDCISLGPGSKFIKITDVQCGPGHGVSIDSLGKYPNEEDVSEVIVRDSTF 159
           N++ ++ +TGDDC+S+G G++ + + +V+CGPGHG+SI SLG+YPNE+ V  V VR    
Sbjct: 252 NLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLI 311

Query: 160 VGTNNGVRIKIWSSSLPSFVTKIAFLNLEMKDVKSPIVIDKKYCLDCNRNKMSNSKVKIR 219
             T+NGVRIK W  S P   + I F ++ M +V  P++ID++YC   +      SKVK+ 
Sbjct: 312 KNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSKVKLS 371

Query: 220 DGDMKIYRGFQRP--------NQVLPCEGIVLGNINLPFKGARQP-TSICNNVKGHAFGK 277
           D  +K  +G            ++ +PC  I L +INL   G   P  S C+N+K    GK
Sbjct: 372 DVTIKNIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPILSGK 431

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6H9K02.5e-5438.93Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S... [more]
P245482.2e-5040.24Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1[more]
Q059672.2e-5038.52Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1[more]
Q397667.1e-4940.00Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1[more]
P353391.6e-4837.55Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023552930.13.20e-14574.91exopolygalacturonase-like [Cucurbita pepo subsp. pepo][more]
XP_023007598.13.91e-13871.38exopolygalacturonase-like [Cucurbita maxima][more]
XP_023552929.11.85e-13672.08exopolygalacturonase-like [Cucurbita pepo subsp. pepo][more]
XP_022932245.11.62e-12884.51exopolygalacturonase-like [Cucurbita moschata][more]
XP_022932248.16.73e-12466.08exopolygalacturonase-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1L8321.89e-13871.38exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111500170 PE=3 SV=1[more]
A0A6J1F1P47.86e-12984.51exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111438609 PE=3 SV=... [more]
A0A6J1F1533.26e-12466.08exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111438612 PE=3 SV=... [more]
A0A6J1L5E93.03e-11371.97exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111500171 PE=3 SV=1[more]
A0A6J1EVU62.26e-11177.51exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111438610 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT3G07840.13.2e-5237.10Pectin lyase-like superfamily protein [more]
AT3G07830.11.6e-5135.69Pectin lyase-like superfamily protein [more]
AT5G48140.11.0e-5035.69Pectin lyase-like superfamily protein [more]
AT2G43890.11.1e-4939.43Pectin lyase-like superfamily protein [more]
AT3G14040.12.1e-4838.62Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 1..275
e-value: 9.8E-63
score: 214.5
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 38..265
e-value: 1.2E-57
score: 195.4
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 3..276
NoneNo IPR availablePANTHERPTHR31375:SF246PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 3..276
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 3..277

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG14g07490.1Cp4.1LG14g07490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity