Cp4.1LG14g06530 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG14g06530 vs. ExPASy Swiss-Prot
Match: O65717 (Cyclic nucleotide-gated ion channel 1 OS=Arabidopsis thaliana OX=3702 GN=CNGC1 PE=1 SV=1)

HSP 1 Score: 383.3 bits (983), Expect = 5.9e-105
Identity = 258/670 (38.51%), Postives = 358/670 (53.43%), Query Frame = 0

Query: 78  ADLSCDLFRS-QSCSREHVAERPSGKIHCLKKGMSKGLRFIKKCIGE----LHVWNEVVV 137
           + +S   +RS +S S      + S K +  K+ +SKG+    K +      L  WN++ V
Sbjct: 41  SSISDKFYRSFESSSARIKLFKRSYKSYSFKEAVSKGIGSTHKILDPQGPFLQRWNKIFV 100

Query: 138 ILCVAAILLDPLFCYILVVDEKKICIEFDQKLRITAIVLRSLIDFGYILVIAFHFRMGYT 197
           + C+ A+ LDPLF Y+ ++D+ K C+  D+K+ ITA VLRS  D  Y+L I F FR G+ 
Sbjct: 101 LACIIAVSLDPLFFYVPIIDDAKKCLGIDKKMEITASVLRSFTDVFYVLHIIFQFRTGFI 160

Query: 198 GP----------YDAKRETLYKIGRRYLLSNFTVHILAVLPLPQVVNLLVIPAAKGSHFS 257
            P           + KRE    I +RYL S+F + ILAVLPLPQ+V L++IP  +GS   
Sbjct: 161 APSSRVFGRGVLVEDKRE----IAKRYLSSHFIIDILAVLPLPQMVILIIIPHMRGSSSL 220

Query: 258 MILRSLQFVLVIQHLPRALRIYKFLKEVRLSRSLFPNSTVGNAIFNLFLYMLSSHAFGAF 317
                L+F++  Q++PR +RIY   KEV  +  +   +    A FNLFLYML+SH FGAF
Sbjct: 221 NTKNMLKFIVFFQYIPRFIRIYPLYKEVTRTSGILTETAWAGAAFNLFLYMLASHVFGAF 280

Query: 318 WYLFAIERKASCLQKRC-GTDPYCPRIHNLSSVERSCIKDCSANASSNDK--------TA 377
           WYLF+IER+  C ++ C   +P C     +S +     +    NA  N+         T 
Sbjct: 281 WYLFSIERETVCWKQACERNNPPC-----ISKLLYCDPETAGGNAFLNESCPIQTPNTTL 340

Query: 378 FNFGIFDDALTSGVVYSTDLICKLCYCCWWGLQNLSSLGQGLKTSQHIWEISFAVSITIA 437
           F+FGIF DAL SGVV S D   K  YC WWGLQNLSSLGQ LKTS +IWEI FAV I+IA
Sbjct: 341 FDFGIFLDALQSGVVESQDFPQKFFYCFWWGLQNLSSLGQNLKTSTYIWEICFAVFISIA 400

Query: 438 GLVLFALLIGNLQTFLQANIARLEELRSKVEDIELWMPSILSRVNQGSKSNNVSMFQILN 497
           GLVLF+ LIGN+QT+LQ+   RLEE+R K  D E WM   L   N   +      ++   
Sbjct: 401 GLVLFSFLIGNMQTYLQSTTTRLEEMRVKRRDAEQWMSHRLLPENLRKRIRRYEQYKWQE 460

Query: 498 GR-----------------------------------QLNEKLIDAVCGYLKPVVYTERN 557
            R                                   +++E+L+DA+C  L+PV+YTE +
Sbjct: 461 TRGVDEENLLSNLPKDLRRDIKRHLCLALLMRVPMFEKMDEQLLDALCDRLQPVLYTEES 520

Query: 558 IIVVKGEPLDKMIFVIHGKLWMYSNDNREDGTIGSSESLTKDNYFGEGLLNWGLKDPLLS 617
            IV +G+P+D+M+F++ GKL   + +    G + +SE L   ++ GE LL W L     S
Sbjct: 521 YIVREGDPVDEMLFIMRGKLLTITTNGGRTGFL-NSEYLGAGDFCGEELLTWALDPHSSS 580

Query: 618 TVPLSPKTVSAHTKVEAFVLSANDLKIFISKFWWLISPELRNHPK-FEEQCKKWAVPLMC 674
            +P+S +TV A  +VEAF L A+DLK   S+F  L S +LR+  + + +Q K WA   + 
Sbjct: 581 NLPISTRTVRALMEVEAFALKADDLKFVASQFRRLHSKQLRHTFRYYSQQWKTWAACFIQ 640

BLAST of Cp4.1LG14g06530 vs. ExPASy Swiss-Prot
Match: Q9LNJ0 (Probable cyclic nucleotide-gated ion channel 10 OS=Arabidopsis thaliana OX=3702 GN=CNGC10 PE=2 SV=2)

HSP 1 Score: 326.6 bits (836), Expect = 6.6e-88
Identity = 211/563 (37.48%), Postives = 303/563 (53.82%), Query Frame = 0

Query: 124 LHVWNEVVVILCVAAILLDPLFCYILVVDEKKICIEFDQKLRITAIVLRSLIDFGYILVI 183
           L  WN++ +  CV A+ +DPLF YI +VD  + C+  D KL I A +LR+LID  YI+ I
Sbjct: 76  LQNWNKIFLFACVVALAIDPLFFYIPIVDSARHCLTLDSKLEIAASLLRTLIDAFYIIHI 135

Query: 184 AFHFRMGYTGPY-----------DAKRETLYKIGRRYLLSNFTVHILAVLPLPQVVNLLV 243
            F FR  Y  P            DAK      I  +YL S F + +L++LPLPQ+V L V
Sbjct: 136 VFQFRTAYIAPSSRVFGRGELVDDAK-----AIALKYLSSYFIIDLLSILPLPQIVVLAV 195

Query: 244 IPAAKGSHFSMILRSLQFVLVIQHLPRALRIYKFLKEVRLSRSLFPNSTVGNAIFNLFLY 303
           IP+       +    L+F ++ Q++PR LR+Y    EV  +  +   +    A +NL LY
Sbjct: 196 IPSVNQPVSLLTKDYLKFSIIAQYVPRILRMYPLYTEVTRTSGIVTETAWAGAAWNLSLY 255

Query: 304 MLSSHAFGAFWYLFAIERKASCLQKRCGTDP-------YCPRIHNLSSVERSCIKDCSAN 363
           ML+SH FGA WYL ++ER+  C Q+ C           YC    N+S+   +        
Sbjct: 256 MLASHVFGALWYLISVEREDRCWQEACEKTKGCNMKFLYCENDRNVSNNFLTTSCPFLDP 315

Query: 364 ASSNDKTAFNFGIFDDALTSGVVYSTDLICKLCYCCWWGLQNLSSLGQGLKTSQHIWEIS 423
               + T FNFGIF DAL SGVV S D   K  YC WWGL+NLS+LGQ L+TS+ + EI 
Sbjct: 316 GDITNSTIFNFGIFTDALKSGVVESHDFWKKFFYCFWWGLRNLSALGQNLQTSKFVGEII 375

Query: 424 FAVSITIAGLVLFALLIGNLQTFLQANIARLEELRSKVEDIELWM-----PSILSR---- 483
           FA+SI I+GLVLFALLIGN+Q +L++   R EE+R +  D E WM     P  L +    
Sbjct: 376 FAISICISGLVLFALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPEDLRKRIRR 435

Query: 484 -----------VNQGSKSNN--------------------VSMFQILNGRQLNEKLIDAV 543
                      V + +   N                    V +F+I     ++E+L+DAV
Sbjct: 436 YEQYRWQETRGVEEETLLRNLPKDLRRDIKRHLCLDLLKKVPLFEI-----MDEQLLDAV 495

Query: 544 CGYLKPVVYTERNIIVVKGEPLDKMIFVIHGKLWMYSNDNREDGTIGSSESLTKDNYFGE 603
           C  L+PV+YTE + ++ +G+P+ +M+FV+ G+L   + +    G   ++ +L   ++ GE
Sbjct: 496 CDRLRPVLYTENSYVIREGDPVGEMLFVMRGRLVSATTNGGRSGFF-NAVNLKASDFCGE 555

Query: 604 GLLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDLKIFISKFWWLISPELRNHPKF- 628
            LL W L     S  P+S +TV A T+VEAF L+A DLK   S+F  L S +L++  +F 
Sbjct: 556 DLLPWALDPQSSSHFPISTRTVQALTEVEAFALTAEDLKSVASQFRRLHSKQLQHTFRFY 615

BLAST of Cp4.1LG14g06530 vs. ExPASy Swiss-Prot
Match: Q9FXH6 (Putative cyclic nucleotide-gated ion channel 8 OS=Arabidopsis thaliana OX=3702 GN=CNGC8 PE=3 SV=2)

HSP 1 Score: 323.2 bits (827), Expect = 7.3e-87
Identity = 202/563 (35.88%), Postives = 296/563 (52.58%), Query Frame = 0

Query: 124 LHVWNEVVVILCVAAILLDPLFCYILVVDEKKICIEFDQKLRITAIVLRSLIDFGYILVI 183
           L +WN + VI C+ A+ +DPLF Y+ +VD  K CI  D KL +T   LR++ID  Y+  +
Sbjct: 105 LLLWNRMFVISCILAVSVDPLFFYLPIVDNSKNCIGIDSKLAVTTTTLRTIIDVFYLTRM 164

Query: 184 AFHFRMGYTGPYD---AKRETLY---KIGRRYLLSNFTVHILAVLPLPQVVNLLVIPAAK 243
           A  FR  Y  P      + E +    KI  RYL   F V  LAVLPLPQ+     +  +K
Sbjct: 165 ALQFRTAYIAPSSRVFGRGELVIDPAKIAERYLTRYFIVDFLAVLPLPQIAVWKFLHGSK 224

Query: 244 GSHFSMILRSLQFVLVIQHLPRALRIYKFLKEVRLSRSLFPNSTVGNAIFNLFLYMLSSH 303
           G+      ++L  +++ Q++PR +R      E++ +   F       A + L  YML+SH
Sbjct: 225 GTDVLPTKQALLHIVITQYIPRFVRFIPLTSELKKTAGAFAEGAWAGAAYYLLWYMLASH 284

Query: 304 AFGAFWYLFAIERKASCLQKRCGTDP---------YCPRIHNLSSVERSCIK-------- 363
             GAFWY+ ++ER  +CL+  C   P         YC     +SS E   IK        
Sbjct: 285 ITGAFWYMLSVERNDTCLRSACKVQPDPKVCVQILYCGS-KLMSSRETDWIKSVPDLFKN 344

Query: 364 DCSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLCYCCWWGLQNLSSLGQGLKTSQH 423
           +CSA    +D++ FN+GI+  A++SG+V ST    K CYC WWGLQNLS+LGQGL+TS +
Sbjct: 345 NCSA---KSDESKFNYGIYSQAVSSGIVSSTTFFSKFCYCLWWGLQNLSTLGQGLQTSTY 404

Query: 424 IWEISFAVSITIAGLVLFALLIGNLQTFLQANIARLEELRSKVEDIELWMPSILSRVNQG 483
             E+ F+++I +AGL+LFALLIGN+QT+LQ+   RLEE+R K  D E WM       N  
Sbjct: 405 PGEVLFSIAIAVAGLLLFALLIGNMQTYLQSLTVRLEEMRIKRRDSEQWMHHRSLPQNLR 464

Query: 484 SKSNNVSMFQILNGR-----------------------------------QLNEKLIDAV 543
            +      ++ L  R                                    ++E+L+DA+
Sbjct: 465 ERVRRYDQYKWLETRGVDEENIVQSLPKDLRRDIKRHLCLNLVRRVPLFANMDERLLDAI 524

Query: 544 CGYLKPVVYTERNIIVVKGEPLDKMIFVIHGKLWMYSNDNREDGTIGSSESLTKDNYFGE 603
           C  LKP +YTE   IV +G+P+++M+F+I G+L   + D    G       L + ++ GE
Sbjct: 525 CERLKPSLYTESTYIVREGDPVNEMLFIIRGRLESVTTDGGRSGFFNRG-LLKEGDFCGE 584

Query: 604 GLLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDLKIFISKFWWLISPELRNHPKF- 628
            LL W L     S +P S +TV A T+VEAF L A +LK   S+F  L S +++   +F 
Sbjct: 585 ELLTWALDPKAGSNLPSSTRTVKALTEVEAFALEAEELKFVASQFRRLHSRQVQQTFRFY 644

BLAST of Cp4.1LG14g06530 vs. ExPASy Swiss-Prot
Match: Q8RWS9 (Probable cyclic nucleotide-gated ion channel 5 OS=Arabidopsis thaliana OX=3702 GN=CNGC5 PE=2 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 3.0e-85
Identity = 221/660 (33.48%), Postives = 341/660 (51.67%), Query Frame = 0

Query: 79  DLSCDLFRSQSCSREHVAERPSGKIH----------CLKKGM---SKGLRFIKKCIG--- 138
           DL   L  S    +++ A   SG++H            KKG+   SKGL+ I + +G   
Sbjct: 13  DLDSRLPSSSVAFQQNYASNFSGQLHPIHASNETSRSFKKGIQKGSKGLKSIGRSLGFGV 72

Query: 139 -------ELHV---------------WNEVVVILCVAAILLDPLFCYILVVDEKKICIEF 198
                  +L V                N++ V  C+ ++ +DP F Y+ V++ +  C+  
Sbjct: 73  YRAVFPEDLKVSEKKIFDPQDKFLLYCNKLFVASCILSVFVDPFFFYLPVINAESKCLGI 132

Query: 199 DQKLRITAIVLRSLIDFGYILVIAFHFRMGYTGPYD---AKRETLY---KIGRRYLLSNF 258
           D+KL ITA  LR+ ID  Y+  +A   R  Y  P      + E +    +I +RYL   F
Sbjct: 133 DRKLAITASTLRTFIDVFYLAHMALQLRTAYIAPSSRVFGRGELVIDPAQIAKRYLQRWF 192

Query: 259 TVHILAVLPLPQVVNLLVIPAAKGSHFSMILRSLQFVLVIQHLPRALRIYKFLKEVRLSR 318
            +  L+VLPLPQ+V    + ++ GS      ++L F++++Q++PR LR+     E++ + 
Sbjct: 193 IIDFLSVLPLPQIVVWRFLQSSNGSDVLATKQALLFIVLVQYIPRFLRVLPLTSELKRTA 252

Query: 319 SLFPNSTVGNAIFNLFLYMLSSHAFGAFWYLFAIERKASCLQK------RCGTD-PYCPR 378
            +F  +    A + L LYML+SH  GAFWYL A+ER  +C Q+       C TD  YC  
Sbjct: 253 GVFAETAWAGAAYYLLLYMLASHIVGAFWYLLALERNDACWQEACIDAGNCSTDFLYCGN 312

Query: 379 IHNL------SSVERSCIKD-CSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLCYC 438
             N+      +  + S +K  C A+   N+   F+FGI+  AL+SG+V S + I K CYC
Sbjct: 313 -QNMDGYAVWNRAKESVLKSKCRADLDDNN-PPFDFGIYTQALSSGIVSSQNFIVKYCYC 372

Query: 439 CWWGLQNLSSLGQGLKTSQHIWEISFAVSITIAGLVLFALLIGNLQTFLQANIARLEELR 498
            WWGLQNLS+LGQGL+TS +  EI F++S+ I+GL+LFALLIGN+QT+LQ+   RLEE+R
Sbjct: 373 LWWGLQNLSTLGQGLETSTYPMEIIFSISLAISGLILFALLIGNMQTYLQSLTIRLEEMR 432

Query: 499 SKVEDIELWMPSILSRVNQGSKSNNVSMFQILNGR------------------------- 558
            K  D E WM   +   +   +      ++ L  R                         
Sbjct: 433 VKRRDSEQWMHHRMLPQDLRERVRRYDQYKWLETRGVDEEYLVQNLPKDLRRDIKRHLCL 492

Query: 559 ----------QLNEKLIDAVCGYLKPVVYTERNIIVVKGEPLDKMIFVIHGKLWMYSNDN 618
                      +++KL+DA+C  LKP ++TE   +V +G+P+D+M+F+I G+L   + D 
Sbjct: 493 ALVRRVPLFKSMDDKLLDAICMRLKPCLFTESTYLVREGDPVDEMLFIIRGRLESVTTDG 552

Query: 619 REDGTIGSSESLTKDNYF-GEGLLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDLK 644
              G    + SL K+  F GE LL W L       +P S +TV A T+VEAF L++ +LK
Sbjct: 553 GRSGFF--NRSLLKEGEFCGEELLTWALDPKSGVNLPSSTRTVKALTEVEAFALTSEELK 612

BLAST of Cp4.1LG14g06530 vs. ExPASy Swiss-Prot
Match: Q9S9N5 (Putative cyclic nucleotide-gated ion channel 7 OS=Arabidopsis thaliana OX=3702 GN=CNGC7 PE=3 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 2.0e-84
Identity = 203/564 (35.99%), Postives = 292/564 (51.77%), Query Frame = 0

Query: 124 LHVWNEVVVILCVAAILLDPLFCYILVVDEK-KICIEFDQKLRITAIVLRSLIDFGYILV 183
           L VWN + VI C+ A+ +DPLF Y+ +VD     CI  D KL +T   LR+++D  Y+  
Sbjct: 98  LLVWNRLFVISCILAVSVDPLFFYLPIVDNSGSSCIGIDTKLAVTTTTLRTIVDVFYLTR 157

Query: 184 IAFHFRMGYTGPYD---AKRETLY---KIGRRYLLSNFTVHILAVLPLPQVVNLLVIPAA 243
           +A  FR  Y  P      + E +    KI  RYL   F V  LAVLPLPQ+     +  +
Sbjct: 158 MALQFRTAYIAPSSRVFGRGELVIDPAKIAERYLTRYFVVDFLAVLPLPQIAVWKFLHGS 217

Query: 244 KGSHFSMILRSLQFVLVIQHLPRALRIYKFLKEVRLSRSLFPNSTVGNAIFNLFLYMLSS 303
           KGS       +L  ++++Q++PR +R      E++ +   F       A + L  YML+S
Sbjct: 218 KGSDVLPTKTALLNIVIVQYIPRFVRFIPLTSELKKTAGAFAEGAWAGAAYYLLWYMLAS 277

Query: 304 HAFGAFWYLFAIERKASCLQKRCGTDP---------YCPRIHNLSSVERSCIK------- 363
           H  GAFWY+ ++ER  +C +  C   P         YC     +SS E   IK       
Sbjct: 278 HITGAFWYMLSVERNDTCWRFACKVQPDPRLCVQILYC-GTKFVSSGETEWIKTVPELLK 337

Query: 364 -DCSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLCYCCWWGLQNLSSLGQGLKTSQ 423
            +CSA A   D + FN+GI+  A++SG+V ST    K CYC WWGLQNLS+LGQGL+TS 
Sbjct: 338 SNCSAKA---DDSKFNYGIYGQAISSGIVSSTTFFSKFCYCLWWGLQNLSTLGQGLQTST 397

Query: 424 HIWEISFAVSITIAGLVLFALLIGNLQTFLQANIARLEELRSKVEDIELWMPSILSRVNQ 483
              E+ F+++I IAGL+LFALLIGN+QT+LQ+   RLEE+R K  D E WM       N 
Sbjct: 398 FPGEVLFSIAIAIAGLLLFALLIGNMQTYLQSLTVRLEEMRIKRRDSEQWMHHRSLPQNL 457

Query: 484 GSKSNNVSMFQILNGR-----------------------------------QLNEKLIDA 543
             +      ++ L  R                                    ++E+L+DA
Sbjct: 458 RERVRRYDQYKWLETRGVDEENIVQSLPKDLRRDIKRHLCLNLVRRVPLFANMDERLLDA 517

Query: 544 VCGYLKPVVYTERNIIVVKGEPLDKMIFVIHGKLWMYSNDNREDGTIGSSESLTKDNYFG 603
           +C  LKP ++TE   IV +G+P+++M+F+I G+L   + D    G       L + ++ G
Sbjct: 518 ICERLKPSLFTESTYIVREGDPVNEMMFIIRGRLESVTTDGGRSGFFNRG-LLKEGDFCG 577

Query: 604 EGLLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDLKIFISKFWWLISPELRNHPKF 628
           E LL W L     S +P S +TV A T+VEAF L A +LK   S+F  L S +++   +F
Sbjct: 578 EELLTWALDPKAGSNLPSSTRTVKALTEVEAFALEAEELKFVASQFRRLHSRQVQQTFRF 637

BLAST of Cp4.1LG14g06530 vs. NCBI nr
Match: XP_022922540.1 (cyclic nucleotide-gated ion channel 1-like [Cucurbita moschata] >XP_022922542.1 cyclic nucleotide-gated ion channel 1-like [Cucurbita moschata])

HSP 1 Score: 1079 bits (2791), Expect = 0.0
Identity = 556/638 (87.15%), Postives = 571/638 (89.50%), Query Frame = 0

Query: 87  SQSCSREHVAERPSGKIHCLKKGMSKGLRFIKKCIGELHVWNEVVVILCVAAILLDPLFC 146
           +QSCS EHVAERPSGKI+CLKK MSKGLRFIKKCIGELHVWNEVVVILCVAAILLDPLFC
Sbjct: 10  TQSCSGEHVAERPSGKINCLKKEMSKGLRFIKKCIGELHVWNEVVVILCVAAILLDPLFC 69

Query: 147 YILVVDEKKICIEFDQKLRITAIVLRSLIDFGYILVIAFHFRMGYTGPYDAKRETLYKIG 206
           YILVVDEKK CIEFDQ LRITAIVLRSLIDFGYIL+IAFHFRMGYTGPYDAK+ETLYKIG
Sbjct: 70  YILVVDEKKNCIEFDQNLRITAIVLRSLIDFGYILIIAFHFRMGYTGPYDAKKETLYKIG 129

Query: 207 RRYLLSNFTVHILAVLPLPQVVNLLVIPAAKGSHFSMILRSLQFVLVIQHLPRALRIYKF 266
           RRYLLS FTV ILAVLPLPQVVNLLVIPAAKGSHFSM LRSLQFVL+IQHLPRALRIYKF
Sbjct: 130 RRYLLSYFTVDILAVLPLPQVVNLLVIPAAKGSHFSMALRSLQFVLIIQHLPRALRIYKF 189

Query: 267 LKEVRLSRSLFPNSTVGNAIFNLFLYMLSSHAFGAFWYLFAIERKASCLQKRCGTDPYCP 326
           LK+VRLS SLFPNST GNA+FNLFLYMLSSHAFGAFWYLFAIERKASCLQKRCG DPYCP
Sbjct: 190 LKKVRLSSSLFPNSTGGNAMFNLFLYMLSSHAFGAFWYLFAIERKASCLQKRCGADPYCP 249

Query: 327 RIHNLSSVERSCIKDCSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLCYCCWWGLQ 386
           R+HNLSSVERSCIKDCSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLCYCCWWGLQ
Sbjct: 250 RMHNLSSVERSCIKDCSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLCYCCWWGLQ 309

Query: 387 NLSSLGQGLKTSQHIWEISFAVSITIAGLVLFALLIGNLQTFLQANIARLEELRSKVEDI 446
           NLSSLGQGLKTSQHIWEI FAVSITIAGLVLFALLIGNLQTFLQANIARLEELR KVEDI
Sbjct: 310 NLSSLGQGLKTSQHIWEIFFAVSITIAGLVLFALLIGNLQTFLQANIARLEELRLKVEDI 369

Query: 447 ELWMP-----------------------------SILSRVNQGSKS-----------NNV 506
           ELW P                             +IL  +    +S           NNV
Sbjct: 370 ELWTPFHFLPRKLRKQIKKCEMRKLRQTRSDDVANILRNLPTDLRSSTVKHLCSPAFNNV 429

Query: 507 SMFQILNGRQLNEKLIDAVCGYLKPVVYTERNIIVVKGEPLDKMIFVIHGKLWMYSNDNR 566
           SMFQILNG+QLNEKLIDAVCGYLKPV YTERNIIV KG+PLDKMIFVIHGKLWMYSNDNR
Sbjct: 430 SMFQILNGQQLNEKLIDAVCGYLKPVEYTERNIIVEKGKPLDKMIFVIHGKLWMYSNDNR 489

Query: 567 EDGTIGSSESLTKDNYFGEGLLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDLKIF 626
           EDGTIGSSESLTKDNYFGE LLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDLKIF
Sbjct: 490 EDGTIGSSESLTKDNYFGEDLLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDLKIF 549

Query: 627 ISKFWWLISPELRNHPKFEEQCKKWAVPLMCAAWYRYKKNK-RRSKIESGSSQPSGTTGI 683
           ISKFWWLIS EL NHPKF+EQCKKWAVPL+CAAWYRYKKNK RRSKIESGSSQPSGTTGI
Sbjct: 550 ISKFWWLISSELSNHPKFKEQCKKWAVPLICAAWYRYKKNKQRRSKIESGSSQPSGTTGI 609

BLAST of Cp4.1LG14g06530 vs. NCBI nr
Match: KAG6576666.1 (Cyclic nucleotide-gated ion channel 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1076 bits (2783), Expect = 0.0
Identity = 556/638 (87.15%), Postives = 567/638 (88.87%), Query Frame = 0

Query: 86  RSQSCSREHVAERPSGKIHCLKKGMSKGLRFIKKCIGELHVWNEVVVILCVAAILLDPLF 145
           R+QSCS EHVAE+ SGKI+CLKK MSKGLRFIKKCIGELHVWNEVVVILCVAAILLDPLF
Sbjct: 9   RTQSCSGEHVAEKASGKINCLKKEMSKGLRFIKKCIGELHVWNEVVVILCVAAILLDPLF 68

Query: 146 CYILVVDEKKICIEFDQKLRITAIVLRSLIDFGYILVIAFHFRMGYTGPYDAKRETLYKI 205
           CYILVVDEKK CIEFDQKLRITAIVLRSLIDFGYIL+IAFHFRMGYTGPYDAK+ETLYKI
Sbjct: 69  CYILVVDEKKNCIEFDQKLRITAIVLRSLIDFGYILIIAFHFRMGYTGPYDAKKETLYKI 128

Query: 206 GRRYLLSNFTVHILAVLPLPQVVNLLVIPAAKGSHFSMILRSLQFVLVIQHLPRALRIYK 265
           GRRYLLS FTV ILAVLPLPQVVNLLVIPAAKGSHFSM L SLQFVLVIQHLPRALRIYK
Sbjct: 129 GRRYLLSYFTVDILAVLPLPQVVNLLVIPAAKGSHFSMALGSLQFVLVIQHLPRALRIYK 188

Query: 266 FLKEVRLSRSLFPNSTVGNAIFNLFLYMLSSHAFGAFWYLFAIERKASCLQKRCGTDPYC 325
           FLK+VRLS SLFPNST GNAIFNLFLYMLSSHAFGAFWYLFAIERKASCLQKRCG DPYC
Sbjct: 189 FLKKVRLSSSLFPNSTGGNAIFNLFLYMLSSHAFGAFWYLFAIERKASCLQKRCGADPYC 248

Query: 326 PRIHNLSSVERSCIKDCSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLCYCCWWGL 385
           PRIHNLSSVERSCIKDCSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLCYCCWWGL
Sbjct: 249 PRIHNLSSVERSCIKDCSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLCYCCWWGL 308

Query: 386 QNLSSLGQGLKTSQHIWEISFAVSITIAGLVLFALLIGNLQTFLQANIARLEELRSKVED 445
           QNLSSLGQGLKTSQHIWEISFAVSITIAGLVLFALLIGNLQTFLQANIARLEELR KVED
Sbjct: 309 QNLSSLGQGLKTSQHIWEISFAVSITIAGLVLFALLIGNLQTFLQANIARLEELRLKVED 368

Query: 446 IELWMP-----------------------------SILSRVNQGSKS-----------NN 505
           IELW P                             +I   +    +S           NN
Sbjct: 369 IELWTPFHFLPRKLRKQIKQCEMRKLRQTRSDDVANIFRNLPTDLRSSTVKHLCSPAFNN 428

Query: 506 VSMFQILNGRQLNEKLIDAVCGYLKPVVYTERNIIVVKGEPLDKMIFVIHGKLWMYSNDN 565
           VSMFQILNG QLNEKLIDAVCGYLKPV YTERNIIV KG+PLDKMIFVIHGKLWMYSNDN
Sbjct: 429 VSMFQILNGPQLNEKLIDAVCGYLKPVEYTERNIIVEKGKPLDKMIFVIHGKLWMYSNDN 488

Query: 566 REDGTIGSSESLTKDNYFGEGLLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDLKI 625
           REDGTIGSSESLTKDNYFGE LLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDLKI
Sbjct: 489 REDGTIGSSESLTKDNYFGEDLLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDLKI 548

Query: 626 FISKFWWLISPELRNHPKFEEQCKKWAVPLMCAAWYRYKKNKRRSKIESGSSQPSGTTGI 683
           FISKFWWLIS ELRNHPK EEQCKKWAVPL+CAAWYRYKKNKRR KIE  SSQPSGTTGI
Sbjct: 549 FISKFWWLISRELRNHPKIEEQCKKWAVPLICAAWYRYKKNKRRPKIERWSSQPSGTTGI 608

BLAST of Cp4.1LG14g06530 vs. NCBI nr
Match: KAG7014716.1 (Cyclic nucleotide-gated ion channel 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1063 bits (2749), Expect = 0.0
Identity = 550/635 (86.61%), Postives = 562/635 (88.50%), Query Frame = 0

Query: 89  SCSREHVAERPSGKIHCLKKGMSKGLRFIKKCIGELHVWNEVVVILCVAAILLDPLFCYI 148
           SCS EHVAE+PSGKI+CLKK  SKGLRFIKKCIGELHVWNEVVVILCVAAILLDPLFCYI
Sbjct: 39  SCSGEHVAEKPSGKINCLKKETSKGLRFIKKCIGELHVWNEVVVILCVAAILLDPLFCYI 98

Query: 149 LVVDEKKICIEFDQKLRITAIVLRSLIDFGYILVIAFHFRMGYTGPYDAKRETLYKIGRR 208
           LVVDEKK CIEFDQKLRITAIVLRSLIDFGYIL+IAFHFRMGYTGPYDAK+ETLYKIGRR
Sbjct: 99  LVVDEKKNCIEFDQKLRITAIVLRSLIDFGYILIIAFHFRMGYTGPYDAKKETLYKIGRR 158

Query: 209 YLLSNFTVHILAVLPLPQVVNLLVIPAAKGSHFSMILRSLQFVLVIQHLPRALRIYKFLK 268
           YLLS FTV ILAVLPLPQVVNLLVIPAAKGSHFSM LRSLQFVL+IQHLPRALRIYKFLK
Sbjct: 159 YLLSYFTVDILAVLPLPQVVNLLVIPAAKGSHFSMALRSLQFVLIIQHLPRALRIYKFLK 218

Query: 269 EVRLSRSLFPNSTVGNAIFNLFLYMLSSHAFGAFWYLFAIERKASCLQKRCGTDPYCPRI 328
           +VRLS SLFPNST GNAIFNLFLYMLSSHAFGAFWYLFAIERKASCLQKRCG DPYCPRI
Sbjct: 219 KVRLSSSLFPNSTGGNAIFNLFLYMLSSHAFGAFWYLFAIERKASCLQKRCGADPYCPRI 278

Query: 329 HNLSSVERSCIKDCSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLCYCCWWGLQNL 388
           HNLSSVERSCIKDCSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLCYCCWWGLQNL
Sbjct: 279 HNLSSVERSCIKDCSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLCYCCWWGLQNL 338

Query: 389 SSLGQGLKTSQHIWEISFAVSITIAGLVLFALLIGNLQTFLQANIARLEELRSKVEDIEL 448
           SSLGQGLKTSQHIWEISFAVSITIAGLVLFALLIGNLQ    ANIARLEELR KVEDIEL
Sbjct: 339 SSLGQGLKTSQHIWEISFAVSITIAGLVLFALLIGNLQ----ANIARLEELRLKVEDIEL 398

Query: 449 WMP-----------------------------SILSRVNQGSKS-----------NNVSM 508
           W P                             +I   +    +S           NNVSM
Sbjct: 399 WTPFHFLPRKLRKQIKQCEMRKLRQTRSDDVANIFRNLPTDLRSSTVKHLCSPAFNNVSM 458

Query: 509 FQILNGRQLNEKLIDAVCGYLKPVVYTERNIIVVKGEPLDKMIFVIHGKLWMYSNDNRED 568
           FQILNG+QLNEKLIDAVCGYLKPV YTERNIIV KG+PLDKMIFVIHGKLWMYSNDNRED
Sbjct: 459 FQILNGQQLNEKLIDAVCGYLKPVEYTERNIIVEKGKPLDKMIFVIHGKLWMYSNDNRED 518

Query: 569 GTIGSSESLTKDNYFGEGLLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDLKIFIS 628
           GTIGSSESLTKDNYFGE LLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDLKIFIS
Sbjct: 519 GTIGSSESLTKDNYFGEDLLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDLKIFIS 578

Query: 629 KFWWLISPELRNHPKFEEQCKKWAVPLMCAAWYRYKKNKRRSKIESGSSQPSGTTGIAHA 683
           KFWWLIS ELRNHPK EEQCKKWAVPL+CAAWYRYKKNKRR KIE  SSQPSGTTGIAHA
Sbjct: 579 KFWWLISRELRNHPKIEEQCKKWAVPLICAAWYRYKKNKRRPKIERWSSQPSGTTGIAHA 638

BLAST of Cp4.1LG14g06530 vs. NCBI nr
Match: XP_022944854.1 (cyclic nucleotide-gated ion channel 1-like isoform X5 [Cucurbita moschata])

HSP 1 Score: 672 bits (1734), Expect = 4.52e-232
Identity = 387/707 (54.74%), Postives = 472/707 (66.76%), Query Frame = 0

Query: 27  GWCYKDIHPVFSKKELVSLWMDKMVPYAFVSHEYVRLPLILLSCQHVIDLYADLSCDLFR 86
           G C+KDIHPV  KKE      D  +     S                +D+ ++ S     
Sbjct: 11  GCCWKDIHPVSGKKEAAMNGEDAAIRIQTSSP---------------VDIRSENSTGTVG 70

Query: 87  SQSCSREHVAERPS--GKIHCLKKGMSKGLRFIKKCIGE----LHVWNEVVVILCVAAIL 146
           +     E  ++  +  G IH  ++  SKGL  IK+ I      LH+WN+V+V+LCV A L
Sbjct: 71  AVPRRFESSSDTLTSFGNIHFKEEVKSKGLSSIKRSIEGNWIFLHLWNDVLVMLCVIAAL 130

Query: 147 LDPLFCYILVVDEKKICIEFDQKLRITAIVLRSLIDFGYILVIAFHFRMGYTGPYDAKRE 206
           LDPLFCYILVVD +K CI FD+KLRITA+VLRSLIDFGYIL+I FHFR+GYT   DA   
Sbjct: 131 LDPLFCYILVVDNEKNCIRFDKKLRITAVVLRSLIDFGYILLIVFHFRIGYTDSKDANNG 190

Query: 207 TLYKIG--RRYLLSNFTVHILAVLPLPQVVNLLVIPAAKGSHFSMILRSLQFVLVIQHLP 266
           TL  I   RRYLLS  TV ILA LPLPQVV +L   AAKGSHF++ +R+L+FVL+IQ+LP
Sbjct: 191 TLSTIATARRYLLSYLTVDILAALPLPQVVIVLAAQAAKGSHFTISIRTLKFVLIIQYLP 250

Query: 267 RALRIYKFLKEVRLSRSLFPNSTVGNAIFNLFLYMLSSHAFGAFWYLFAIERKASCLQKR 326
           R  R+Y FLK+VR S  + P+S    AIFNLFLYML+SH FGAFWYLF+IERKA+CLQ R
Sbjct: 251 RVFRVYLFLKKVRWSSGILPDSAAAKAIFNLFLYMLASHVFGAFWYLFSIERKATCLQSR 310

Query: 327 CGTDPYCPRIHNLSSVERSCIKDCSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLC 386
           C + PYCPR +N++  E SC+ +CS  ASSND   FNFGIFDDA    VV STD I K+ 
Sbjct: 311 CHSHPYCPRKYNITE-EGSCLDNCSRKASSNDTLPFNFGIFDDAFKYDVVNSTDFIWKVS 370

Query: 387 YCCWWGLQNLSSLGQGLKTSQHIWEISFAVSITIAGLVLFALLIGNLQTFLQANIARLEE 446
           YC WWGLQNLSSLGQGL+TS+HIWEI FAVSITIAGLVLFALLIGNLQTFLQ+ IARLEE
Sbjct: 371 YCYWWGLQNLSSLGQGLRTSKHIWEIYFAVSITIAGLVLFALLIGNLQTFLQSTIARLEE 430

Query: 447 LRSKVEDIELWMP------SILSRVN-----QGSKSNNVSMFQILNG------------- 506
           +R K +DIELWM        +  R+      +  K+  V +  IL+              
Sbjct: 431 MRLKGQDIELWMAYHSLPRDLRKRIKLYEKYKWRKTRGVDVANILHNLPRDLRRDTTRHL 490

Query: 507 -----------RQLNEKLIDAVCGYLKPVVYTERNIIVVKGEPLDKMIFVIHGKLWMYSN 566
                      + ++EK +DAVCG+LKP++Y ERN IV +GEPLD+MIF+IHGKLW+YSN
Sbjct: 491 CLSAIKSVSMFQNMDEKFLDAVCGFLKPMLYIERNFIVREGEPLDEMIFIIHGKLWIYSN 550

Query: 567 DNREDGTIGSSESLTKDNYFGEGLLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDL 626
            NR+  T GSSESL K + FGE LL W LKDPLLSTVP+S KTVS HTKVEAFVLSA+DL
Sbjct: 551 SNRDGETFGSSESLKKGDVFGEELLKWVLKDPLLSTVPISTKTVSTHTKVEAFVLSAHDL 610

Query: 627 KIFISKFWWLISPELRNHPKFEEQCKKWAVPLMCAAWYRYKKNKRRSK-------IESGS 683
           K  +SKFWWLIS E  N P F+E+    A  ++ AAW RY K+KR  +        ESG+
Sbjct: 611 KNVVSKFWWLISQEHGNDPNFKERWAPLAALVVQAAWRRYFKSKREREKSQLSLATESGN 670

BLAST of Cp4.1LG14g06530 vs. NCBI nr
Match: XP_022944852.1 (cyclic nucleotide-gated ion channel 1-like isoform X4 [Cucurbita moschata])

HSP 1 Score: 672 bits (1734), Expect = 5.73e-232
Identity = 387/707 (54.74%), Postives = 472/707 (66.76%), Query Frame = 0

Query: 27  GWCYKDIHPVFSKKELVSLWMDKMVPYAFVSHEYVRLPLILLSCQHVIDLYADLSCDLFR 86
           G C+KDIHPV  KKE      D  +     S                +D+ ++ S     
Sbjct: 11  GCCWKDIHPVSGKKEAAMNGEDAAIRIQTSSP---------------VDIRSENSTGTVG 70

Query: 87  SQSCSREHVAERPS--GKIHCLKKGMSKGLRFIKKCIGE----LHVWNEVVVILCVAAIL 146
           +     E  ++  +  G IH  ++  SKGL  IK+ I      LH+WN+V+V+LCV A L
Sbjct: 71  AVPRRFESSSDTLTSFGNIHFKEEVKSKGLSSIKRSIEGNWIFLHLWNDVLVMLCVIAAL 130

Query: 147 LDPLFCYILVVDEKKICIEFDQKLRITAIVLRSLIDFGYILVIAFHFRMGYTGPYDAKRE 206
           LDPLFCYILVVD +K CI FD+KLRITA+VLRSLIDFGYIL+I FHFR+GYT   DA   
Sbjct: 131 LDPLFCYILVVDNEKNCIRFDKKLRITAVVLRSLIDFGYILLIVFHFRIGYTDSKDANNG 190

Query: 207 TLYKIG--RRYLLSNFTVHILAVLPLPQVVNLLVIPAAKGSHFSMILRSLQFVLVIQHLP 266
           TL  I   RRYLLS  TV ILA LPLPQVV +L   AAKGSHF++ +R+L+FVL+IQ+LP
Sbjct: 191 TLSTIATARRYLLSYLTVDILAALPLPQVVIVLAAQAAKGSHFTISIRTLKFVLIIQYLP 250

Query: 267 RALRIYKFLKEVRLSRSLFPNSTVGNAIFNLFLYMLSSHAFGAFWYLFAIERKASCLQKR 326
           R  R+Y FLK+VR S  + P+S    AIFNLFLYML+SH FGAFWYLF+IERKA+CLQ R
Sbjct: 251 RVFRVYLFLKKVRWSSGILPDSAAAKAIFNLFLYMLASHVFGAFWYLFSIERKATCLQSR 310

Query: 327 CGTDPYCPRIHNLSSVERSCIKDCSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLC 386
           C + PYCPR +N++  E SC+ +CS  ASSND   FNFGIFDDA    VV STD I K+ 
Sbjct: 311 CHSHPYCPRKYNITE-EGSCLDNCSRKASSNDTLPFNFGIFDDAFKYDVVNSTDFIWKVS 370

Query: 387 YCCWWGLQNLSSLGQGLKTSQHIWEISFAVSITIAGLVLFALLIGNLQTFLQANIARLEE 446
           YC WWGLQNLSSLGQGL+TS+HIWEI FAVSITIAGLVLFALLIGNLQTFLQ+ IARLEE
Sbjct: 371 YCYWWGLQNLSSLGQGLRTSKHIWEIYFAVSITIAGLVLFALLIGNLQTFLQSTIARLEE 430

Query: 447 LRSKVEDIELWMP------SILSRVN-----QGSKSNNVSMFQILNG------------- 506
           +R K +DIELWM        +  R+      +  K+  V +  IL+              
Sbjct: 431 MRLKGQDIELWMAYHSLPRDLRKRIKLYEKYKWRKTRGVDVANILHNLPRDLRRDTTRHL 490

Query: 507 -----------RQLNEKLIDAVCGYLKPVVYTERNIIVVKGEPLDKMIFVIHGKLWMYSN 566
                      + ++EK +DAVCG+LKP++Y ERN IV +GEPLD+MIF+IHGKLW+YSN
Sbjct: 491 CLSAIKSVSMFQNMDEKFLDAVCGFLKPMLYIERNFIVREGEPLDEMIFIIHGKLWIYSN 550

Query: 567 DNREDGTIGSSESLTKDNYFGEGLLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDL 626
            NR+  T GSSESL K + FGE LL W LKDPLLSTVP+S KTVS HTKVEAFVLSA+DL
Sbjct: 551 SNRDGETFGSSESLKKGDVFGEELLKWVLKDPLLSTVPISTKTVSTHTKVEAFVLSAHDL 610

Query: 627 KIFISKFWWLISPELRNHPKFEEQCKKWAVPLMCAAWYRYKKNKRRSK-------IESGS 683
           K  +SKFWWLIS E  N P F+E+    A  ++ AAW RY K+KR  +        ESG+
Sbjct: 611 KNVVSKFWWLISQEHGNDPNFKERWAPLAALVVQAAWRRYFKSKREREKSQLSLATESGN 670

BLAST of Cp4.1LG14g06530 vs. ExPASy TrEMBL
Match: A0A6J1E924 (cyclic nucleotide-gated ion channel 1-like OS=Cucurbita moschata OX=3662 GN=LOC111430508 PE=4 SV=1)

HSP 1 Score: 1079 bits (2791), Expect = 0.0
Identity = 556/638 (87.15%), Postives = 571/638 (89.50%), Query Frame = 0

Query: 87  SQSCSREHVAERPSGKIHCLKKGMSKGLRFIKKCIGELHVWNEVVVILCVAAILLDPLFC 146
           +QSCS EHVAERPSGKI+CLKK MSKGLRFIKKCIGELHVWNEVVVILCVAAILLDPLFC
Sbjct: 10  TQSCSGEHVAERPSGKINCLKKEMSKGLRFIKKCIGELHVWNEVVVILCVAAILLDPLFC 69

Query: 147 YILVVDEKKICIEFDQKLRITAIVLRSLIDFGYILVIAFHFRMGYTGPYDAKRETLYKIG 206
           YILVVDEKK CIEFDQ LRITAIVLRSLIDFGYIL+IAFHFRMGYTGPYDAK+ETLYKIG
Sbjct: 70  YILVVDEKKNCIEFDQNLRITAIVLRSLIDFGYILIIAFHFRMGYTGPYDAKKETLYKIG 129

Query: 207 RRYLLSNFTVHILAVLPLPQVVNLLVIPAAKGSHFSMILRSLQFVLVIQHLPRALRIYKF 266
           RRYLLS FTV ILAVLPLPQVVNLLVIPAAKGSHFSM LRSLQFVL+IQHLPRALRIYKF
Sbjct: 130 RRYLLSYFTVDILAVLPLPQVVNLLVIPAAKGSHFSMALRSLQFVLIIQHLPRALRIYKF 189

Query: 267 LKEVRLSRSLFPNSTVGNAIFNLFLYMLSSHAFGAFWYLFAIERKASCLQKRCGTDPYCP 326
           LK+VRLS SLFPNST GNA+FNLFLYMLSSHAFGAFWYLFAIERKASCLQKRCG DPYCP
Sbjct: 190 LKKVRLSSSLFPNSTGGNAMFNLFLYMLSSHAFGAFWYLFAIERKASCLQKRCGADPYCP 249

Query: 327 RIHNLSSVERSCIKDCSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLCYCCWWGLQ 386
           R+HNLSSVERSCIKDCSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLCYCCWWGLQ
Sbjct: 250 RMHNLSSVERSCIKDCSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLCYCCWWGLQ 309

Query: 387 NLSSLGQGLKTSQHIWEISFAVSITIAGLVLFALLIGNLQTFLQANIARLEELRSKVEDI 446
           NLSSLGQGLKTSQHIWEI FAVSITIAGLVLFALLIGNLQTFLQANIARLEELR KVEDI
Sbjct: 310 NLSSLGQGLKTSQHIWEIFFAVSITIAGLVLFALLIGNLQTFLQANIARLEELRLKVEDI 369

Query: 447 ELWMP-----------------------------SILSRVNQGSKS-----------NNV 506
           ELW P                             +IL  +    +S           NNV
Sbjct: 370 ELWTPFHFLPRKLRKQIKKCEMRKLRQTRSDDVANILRNLPTDLRSSTVKHLCSPAFNNV 429

Query: 507 SMFQILNGRQLNEKLIDAVCGYLKPVVYTERNIIVVKGEPLDKMIFVIHGKLWMYSNDNR 566
           SMFQILNG+QLNEKLIDAVCGYLKPV YTERNIIV KG+PLDKMIFVIHGKLWMYSNDNR
Sbjct: 430 SMFQILNGQQLNEKLIDAVCGYLKPVEYTERNIIVEKGKPLDKMIFVIHGKLWMYSNDNR 489

Query: 567 EDGTIGSSESLTKDNYFGEGLLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDLKIF 626
           EDGTIGSSESLTKDNYFGE LLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDLKIF
Sbjct: 490 EDGTIGSSESLTKDNYFGEDLLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDLKIF 549

Query: 627 ISKFWWLISPELRNHPKFEEQCKKWAVPLMCAAWYRYKKNK-RRSKIESGSSQPSGTTGI 683
           ISKFWWLIS EL NHPKF+EQCKKWAVPL+CAAWYRYKKNK RRSKIESGSSQPSGTTGI
Sbjct: 550 ISKFWWLISSELSNHPKFKEQCKKWAVPLICAAWYRYKKNKQRRSKIESGSSQPSGTTGI 609

BLAST of Cp4.1LG14g06530 vs. ExPASy TrEMBL
Match: A0A6J1FZB6 (cyclic nucleotide-gated ion channel 1-like isoform X5 OS=Cucurbita moschata OX=3662 GN=LOC111449257 PE=4 SV=1)

HSP 1 Score: 672 bits (1734), Expect = 2.19e-232
Identity = 387/707 (54.74%), Postives = 472/707 (66.76%), Query Frame = 0

Query: 27  GWCYKDIHPVFSKKELVSLWMDKMVPYAFVSHEYVRLPLILLSCQHVIDLYADLSCDLFR 86
           G C+KDIHPV  KKE      D  +     S                +D+ ++ S     
Sbjct: 11  GCCWKDIHPVSGKKEAAMNGEDAAIRIQTSSP---------------VDIRSENSTGTVG 70

Query: 87  SQSCSREHVAERPS--GKIHCLKKGMSKGLRFIKKCIGE----LHVWNEVVVILCVAAIL 146
           +     E  ++  +  G IH  ++  SKGL  IK+ I      LH+WN+V+V+LCV A L
Sbjct: 71  AVPRRFESSSDTLTSFGNIHFKEEVKSKGLSSIKRSIEGNWIFLHLWNDVLVMLCVIAAL 130

Query: 147 LDPLFCYILVVDEKKICIEFDQKLRITAIVLRSLIDFGYILVIAFHFRMGYTGPYDAKRE 206
           LDPLFCYILVVD +K CI FD+KLRITA+VLRSLIDFGYIL+I FHFR+GYT   DA   
Sbjct: 131 LDPLFCYILVVDNEKNCIRFDKKLRITAVVLRSLIDFGYILLIVFHFRIGYTDSKDANNG 190

Query: 207 TLYKIG--RRYLLSNFTVHILAVLPLPQVVNLLVIPAAKGSHFSMILRSLQFVLVIQHLP 266
           TL  I   RRYLLS  TV ILA LPLPQVV +L   AAKGSHF++ +R+L+FVL+IQ+LP
Sbjct: 191 TLSTIATARRYLLSYLTVDILAALPLPQVVIVLAAQAAKGSHFTISIRTLKFVLIIQYLP 250

Query: 267 RALRIYKFLKEVRLSRSLFPNSTVGNAIFNLFLYMLSSHAFGAFWYLFAIERKASCLQKR 326
           R  R+Y FLK+VR S  + P+S    AIFNLFLYML+SH FGAFWYLF+IERKA+CLQ R
Sbjct: 251 RVFRVYLFLKKVRWSSGILPDSAAAKAIFNLFLYMLASHVFGAFWYLFSIERKATCLQSR 310

Query: 327 CGTDPYCPRIHNLSSVERSCIKDCSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLC 386
           C + PYCPR +N++  E SC+ +CS  ASSND   FNFGIFDDA    VV STD I K+ 
Sbjct: 311 CHSHPYCPRKYNITE-EGSCLDNCSRKASSNDTLPFNFGIFDDAFKYDVVNSTDFIWKVS 370

Query: 387 YCCWWGLQNLSSLGQGLKTSQHIWEISFAVSITIAGLVLFALLIGNLQTFLQANIARLEE 446
           YC WWGLQNLSSLGQGL+TS+HIWEI FAVSITIAGLVLFALLIGNLQTFLQ+ IARLEE
Sbjct: 371 YCYWWGLQNLSSLGQGLRTSKHIWEIYFAVSITIAGLVLFALLIGNLQTFLQSTIARLEE 430

Query: 447 LRSKVEDIELWMP------SILSRVN-----QGSKSNNVSMFQILNG------------- 506
           +R K +DIELWM        +  R+      +  K+  V +  IL+              
Sbjct: 431 MRLKGQDIELWMAYHSLPRDLRKRIKLYEKYKWRKTRGVDVANILHNLPRDLRRDTTRHL 490

Query: 507 -----------RQLNEKLIDAVCGYLKPVVYTERNIIVVKGEPLDKMIFVIHGKLWMYSN 566
                      + ++EK +DAVCG+LKP++Y ERN IV +GEPLD+MIF+IHGKLW+YSN
Sbjct: 491 CLSAIKSVSMFQNMDEKFLDAVCGFLKPMLYIERNFIVREGEPLDEMIFIIHGKLWIYSN 550

Query: 567 DNREDGTIGSSESLTKDNYFGEGLLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDL 626
            NR+  T GSSESL K + FGE LL W LKDPLLSTVP+S KTVS HTKVEAFVLSA+DL
Sbjct: 551 SNRDGETFGSSESLKKGDVFGEELLKWVLKDPLLSTVPISTKTVSTHTKVEAFVLSAHDL 610

Query: 627 KIFISKFWWLISPELRNHPKFEEQCKKWAVPLMCAAWYRYKKNKRRSK-------IESGS 683
           K  +SKFWWLIS E  N P F+E+    A  ++ AAW RY K+KR  +        ESG+
Sbjct: 611 KNVVSKFWWLISQEHGNDPNFKERWAPLAALVVQAAWRRYFKSKREREKSQLSLATESGN 670

BLAST of Cp4.1LG14g06530 vs. ExPASy TrEMBL
Match: A0A6J1FZA3 (cyclic nucleotide-gated ion channel 1-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111449257 PE=4 SV=1)

HSP 1 Score: 672 bits (1734), Expect = 2.78e-232
Identity = 387/707 (54.74%), Postives = 472/707 (66.76%), Query Frame = 0

Query: 27  GWCYKDIHPVFSKKELVSLWMDKMVPYAFVSHEYVRLPLILLSCQHVIDLYADLSCDLFR 86
           G C+KDIHPV  KKE      D  +     S                +D+ ++ S     
Sbjct: 11  GCCWKDIHPVSGKKEAAMNGEDAAIRIQTSSP---------------VDIRSENSTGTVG 70

Query: 87  SQSCSREHVAERPS--GKIHCLKKGMSKGLRFIKKCIGE----LHVWNEVVVILCVAAIL 146
           +     E  ++  +  G IH  ++  SKGL  IK+ I      LH+WN+V+V+LCV A L
Sbjct: 71  AVPRRFESSSDTLTSFGNIHFKEEVKSKGLSSIKRSIEGNWIFLHLWNDVLVMLCVIAAL 130

Query: 147 LDPLFCYILVVDEKKICIEFDQKLRITAIVLRSLIDFGYILVIAFHFRMGYTGPYDAKRE 206
           LDPLFCYILVVD +K CI FD+KLRITA+VLRSLIDFGYIL+I FHFR+GYT   DA   
Sbjct: 131 LDPLFCYILVVDNEKNCIRFDKKLRITAVVLRSLIDFGYILLIVFHFRIGYTDSKDANNG 190

Query: 207 TLYKIG--RRYLLSNFTVHILAVLPLPQVVNLLVIPAAKGSHFSMILRSLQFVLVIQHLP 266
           TL  I   RRYLLS  TV ILA LPLPQVV +L   AAKGSHF++ +R+L+FVL+IQ+LP
Sbjct: 191 TLSTIATARRYLLSYLTVDILAALPLPQVVIVLAAQAAKGSHFTISIRTLKFVLIIQYLP 250

Query: 267 RALRIYKFLKEVRLSRSLFPNSTVGNAIFNLFLYMLSSHAFGAFWYLFAIERKASCLQKR 326
           R  R+Y FLK+VR S  + P+S    AIFNLFLYML+SH FGAFWYLF+IERKA+CLQ R
Sbjct: 251 RVFRVYLFLKKVRWSSGILPDSAAAKAIFNLFLYMLASHVFGAFWYLFSIERKATCLQSR 310

Query: 327 CGTDPYCPRIHNLSSVERSCIKDCSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLC 386
           C + PYCPR +N++  E SC+ +CS  ASSND   FNFGIFDDA    VV STD I K+ 
Sbjct: 311 CHSHPYCPRKYNITE-EGSCLDNCSRKASSNDTLPFNFGIFDDAFKYDVVNSTDFIWKVS 370

Query: 387 YCCWWGLQNLSSLGQGLKTSQHIWEISFAVSITIAGLVLFALLIGNLQTFLQANIARLEE 446
           YC WWGLQNLSSLGQGL+TS+HIWEI FAVSITIAGLVLFALLIGNLQTFLQ+ IARLEE
Sbjct: 371 YCYWWGLQNLSSLGQGLRTSKHIWEIYFAVSITIAGLVLFALLIGNLQTFLQSTIARLEE 430

Query: 447 LRSKVEDIELWMP------SILSRVN-----QGSKSNNVSMFQILNG------------- 506
           +R K +DIELWM        +  R+      +  K+  V +  IL+              
Sbjct: 431 MRLKGQDIELWMAYHSLPRDLRKRIKLYEKYKWRKTRGVDVANILHNLPRDLRRDTTRHL 490

Query: 507 -----------RQLNEKLIDAVCGYLKPVVYTERNIIVVKGEPLDKMIFVIHGKLWMYSN 566
                      + ++EK +DAVCG+LKP++Y ERN IV +GEPLD+MIF+IHGKLW+YSN
Sbjct: 491 CLSAIKSVSMFQNMDEKFLDAVCGFLKPMLYIERNFIVREGEPLDEMIFIIHGKLWIYSN 550

Query: 567 DNREDGTIGSSESLTKDNYFGEGLLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDL 626
            NR+  T GSSESL K + FGE LL W LKDPLLSTVP+S KTVS HTKVEAFVLSA+DL
Sbjct: 551 SNRDGETFGSSESLKKGDVFGEELLKWVLKDPLLSTVPISTKTVSTHTKVEAFVLSAHDL 610

Query: 627 KIFISKFWWLISPELRNHPKFEEQCKKWAVPLMCAAWYRYKKNKRRSK-------IESGS 683
           K  +SKFWWLIS E  N P F+E+    A  ++ AAW RY K+KR  +        ESG+
Sbjct: 611 KNVVSKFWWLISQEHGNDPNFKERWAPLAALVVQAAWRRYFKSKREREKSQLSLATESGN 670

BLAST of Cp4.1LG14g06530 vs. ExPASy TrEMBL
Match: A0A6J1FZ71 (cyclic nucleotide-gated ion channel 1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111449257 PE=4 SV=1)

HSP 1 Score: 672 bits (1734), Expect = 4.31e-232
Identity = 387/707 (54.74%), Postives = 472/707 (66.76%), Query Frame = 0

Query: 27  GWCYKDIHPVFSKKELVSLWMDKMVPYAFVSHEYVRLPLILLSCQHVIDLYADLSCDLFR 86
           G C+KDIHPV  KKE      D  +     S                +D+ ++ S     
Sbjct: 11  GCCWKDIHPVSGKKEAAMNGEDAAIRIQTSSP---------------VDIRSENSTGTVG 70

Query: 87  SQSCSREHVAERPS--GKIHCLKKGMSKGLRFIKKCIGE----LHVWNEVVVILCVAAIL 146
           +     E  ++  +  G IH  ++  SKGL  IK+ I      LH+WN+V+V+LCV A L
Sbjct: 71  AVPRRFESSSDTLTSFGNIHFKEEVKSKGLSSIKRSIEGNWIFLHLWNDVLVMLCVIAAL 130

Query: 147 LDPLFCYILVVDEKKICIEFDQKLRITAIVLRSLIDFGYILVIAFHFRMGYTGPYDAKRE 206
           LDPLFCYILVVD +K CI FD+KLRITA+VLRSLIDFGYIL+I FHFR+GYT   DA   
Sbjct: 131 LDPLFCYILVVDNEKNCIRFDKKLRITAVVLRSLIDFGYILLIVFHFRIGYTDSKDANNG 190

Query: 207 TLYKIG--RRYLLSNFTVHILAVLPLPQVVNLLVIPAAKGSHFSMILRSLQFVLVIQHLP 266
           TL  I   RRYLLS  TV ILA LPLPQVV +L   AAKGSHF++ +R+L+FVL+IQ+LP
Sbjct: 191 TLSTIATARRYLLSYLTVDILAALPLPQVVIVLAAQAAKGSHFTISIRTLKFVLIIQYLP 250

Query: 267 RALRIYKFLKEVRLSRSLFPNSTVGNAIFNLFLYMLSSHAFGAFWYLFAIERKASCLQKR 326
           R  R+Y FLK+VR S  + P+S    AIFNLFLYML+SH FGAFWYLF+IERKA+CLQ R
Sbjct: 251 RVFRVYLFLKKVRWSSGILPDSAAAKAIFNLFLYMLASHVFGAFWYLFSIERKATCLQSR 310

Query: 327 CGTDPYCPRIHNLSSVERSCIKDCSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLC 386
           C + PYCPR +N++  E SC+ +CS  ASSND   FNFGIFDDA    VV STD I K+ 
Sbjct: 311 CHSHPYCPRKYNITE-EGSCLDNCSRKASSNDTLPFNFGIFDDAFKYDVVNSTDFIWKVS 370

Query: 387 YCCWWGLQNLSSLGQGLKTSQHIWEISFAVSITIAGLVLFALLIGNLQTFLQANIARLEE 446
           YC WWGLQNLSSLGQGL+TS+HIWEI FAVSITIAGLVLFALLIGNLQTFLQ+ IARLEE
Sbjct: 371 YCYWWGLQNLSSLGQGLRTSKHIWEIYFAVSITIAGLVLFALLIGNLQTFLQSTIARLEE 430

Query: 447 LRSKVEDIELWMP------SILSRVN-----QGSKSNNVSMFQILNG------------- 506
           +R K +DIELWM        +  R+      +  K+  V +  IL+              
Sbjct: 431 MRLKGQDIELWMAYHSLPRDLRKRIKLYEKYKWRKTRGVDVANILHNLPRDLRRDTTRHL 490

Query: 507 -----------RQLNEKLIDAVCGYLKPVVYTERNIIVVKGEPLDKMIFVIHGKLWMYSN 566
                      + ++EK +DAVCG+LKP++Y ERN IV +GEPLD+MIF+IHGKLW+YSN
Sbjct: 491 CLSAIKSVSMFQNMDEKFLDAVCGFLKPMLYIERNFIVREGEPLDEMIFIIHGKLWIYSN 550

Query: 567 DNREDGTIGSSESLTKDNYFGEGLLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDL 626
            NR+  T GSSESL K + FGE LL W LKDPLLSTVP+S KTVS HTKVEAFVLSA+DL
Sbjct: 551 SNRDGETFGSSESLKKGDVFGEELLKWVLKDPLLSTVPISTKTVSTHTKVEAFVLSAHDL 610

Query: 627 KIFISKFWWLISPELRNHPKFEEQCKKWAVPLMCAAWYRYKKNKRRSK-------IESGS 683
           K  +SKFWWLIS E  N P F+E+    A  ++ AAW RY K+KR  +        ESG+
Sbjct: 611 KNVVSKFWWLISQEHGNDPNFKERWAPLAALVVQAAWRRYFKSKREREKSQLSLATESGN 670

BLAST of Cp4.1LG14g06530 vs. ExPASy TrEMBL
Match: A0A6J1FZ80 (cyclic nucleotide-gated ion channel 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449257 PE=4 SV=1)

HSP 1 Score: 672 bits (1734), Expect = 4.61e-232
Identity = 387/707 (54.74%), Postives = 472/707 (66.76%), Query Frame = 0

Query: 27  GWCYKDIHPVFSKKELVSLWMDKMVPYAFVSHEYVRLPLILLSCQHVIDLYADLSCDLFR 86
           G C+KDIHPV  KKE      D  +     S                +D+ ++ S     
Sbjct: 11  GCCWKDIHPVSGKKEAAMNGEDAAIRIQTSSP---------------VDIRSENSTGTVG 70

Query: 87  SQSCSREHVAERPS--GKIHCLKKGMSKGLRFIKKCIGE----LHVWNEVVVILCVAAIL 146
           +     E  ++  +  G IH  ++  SKGL  IK+ I      LH+WN+V+V+LCV A L
Sbjct: 71  AVPRRFESSSDTLTSFGNIHFKEEVKSKGLSSIKRSIEGNWIFLHLWNDVLVMLCVIAAL 130

Query: 147 LDPLFCYILVVDEKKICIEFDQKLRITAIVLRSLIDFGYILVIAFHFRMGYTGPYDAKRE 206
           LDPLFCYILVVD +K CI FD+KLRITA+VLRSLIDFGYIL+I FHFR+GYT   DA   
Sbjct: 131 LDPLFCYILVVDNEKNCIRFDKKLRITAVVLRSLIDFGYILLIVFHFRIGYTDSKDANNG 190

Query: 207 TLYKIG--RRYLLSNFTVHILAVLPLPQVVNLLVIPAAKGSHFSMILRSLQFVLVIQHLP 266
           TL  I   RRYLLS  TV ILA LPLPQVV +L   AAKGSHF++ +R+L+FVL+IQ+LP
Sbjct: 191 TLSTIATARRYLLSYLTVDILAALPLPQVVIVLAAQAAKGSHFTISIRTLKFVLIIQYLP 250

Query: 267 RALRIYKFLKEVRLSRSLFPNSTVGNAIFNLFLYMLSSHAFGAFWYLFAIERKASCLQKR 326
           R  R+Y FLK+VR S  + P+S    AIFNLFLYML+SH FGAFWYLF+IERKA+CLQ R
Sbjct: 251 RVFRVYLFLKKVRWSSGILPDSAAAKAIFNLFLYMLASHVFGAFWYLFSIERKATCLQSR 310

Query: 327 CGTDPYCPRIHNLSSVERSCIKDCSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLC 386
           C + PYCPR +N++  E SC+ +CS  ASSND   FNFGIFDDA    VV STD I K+ 
Sbjct: 311 CHSHPYCPRKYNITE-EGSCLDNCSRKASSNDTLPFNFGIFDDAFKYDVVNSTDFIWKVS 370

Query: 387 YCCWWGLQNLSSLGQGLKTSQHIWEISFAVSITIAGLVLFALLIGNLQTFLQANIARLEE 446
           YC WWGLQNLSSLGQGL+TS+HIWEI FAVSITIAGLVLFALLIGNLQTFLQ+ IARLEE
Sbjct: 371 YCYWWGLQNLSSLGQGLRTSKHIWEIYFAVSITIAGLVLFALLIGNLQTFLQSTIARLEE 430

Query: 447 LRSKVEDIELWMP------SILSRVN-----QGSKSNNVSMFQILNG------------- 506
           +R K +DIELWM        +  R+      +  K+  V +  IL+              
Sbjct: 431 MRLKGQDIELWMAYHSLPRDLRKRIKLYEKYKWRKTRGVDVANILHNLPRDLRRDTTRHL 490

Query: 507 -----------RQLNEKLIDAVCGYLKPVVYTERNIIVVKGEPLDKMIFVIHGKLWMYSN 566
                      + ++EK +DAVCG+LKP++Y ERN IV +GEPLD+MIF+IHGKLW+YSN
Sbjct: 491 CLSAIKSVSMFQNMDEKFLDAVCGFLKPMLYIERNFIVREGEPLDEMIFIIHGKLWIYSN 550

Query: 567 DNREDGTIGSSESLTKDNYFGEGLLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDL 626
            NR+  T GSSESL K + FGE LL W LKDPLLSTVP+S KTVS HTKVEAFVLSA+DL
Sbjct: 551 SNRDGETFGSSESLKKGDVFGEELLKWVLKDPLLSTVPISTKTVSTHTKVEAFVLSAHDL 610

Query: 627 KIFISKFWWLISPELRNHPKFEEQCKKWAVPLMCAAWYRYKKNKRRSK-------IESGS 683
           K  +SKFWWLIS E  N P F+E+    A  ++ AAW RY K+KR  +        ESG+
Sbjct: 611 KNVVSKFWWLISQEHGNDPNFKERWAPLAALVVQAAWRRYFKSKREREKSQLSLATESGN 670

BLAST of Cp4.1LG14g06530 vs. TAIR 10
Match: AT5G53130.1 (cyclic nucleotide gated channel 1 )

HSP 1 Score: 383.3 bits (983), Expect = 4.2e-106
Identity = 258/670 (38.51%), Postives = 358/670 (53.43%), Query Frame = 0

Query: 78  ADLSCDLFRS-QSCSREHVAERPSGKIHCLKKGMSKGLRFIKKCIGE----LHVWNEVVV 137
           + +S   +RS +S S      + S K +  K+ +SKG+    K +      L  WN++ V
Sbjct: 41  SSISDKFYRSFESSSARIKLFKRSYKSYSFKEAVSKGIGSTHKILDPQGPFLQRWNKIFV 100

Query: 138 ILCVAAILLDPLFCYILVVDEKKICIEFDQKLRITAIVLRSLIDFGYILVIAFHFRMGYT 197
           + C+ A+ LDPLF Y+ ++D+ K C+  D+K+ ITA VLRS  D  Y+L I F FR G+ 
Sbjct: 101 LACIIAVSLDPLFFYVPIIDDAKKCLGIDKKMEITASVLRSFTDVFYVLHIIFQFRTGFI 160

Query: 198 GP----------YDAKRETLYKIGRRYLLSNFTVHILAVLPLPQVVNLLVIPAAKGSHFS 257
            P           + KRE    I +RYL S+F + ILAVLPLPQ+V L++IP  +GS   
Sbjct: 161 APSSRVFGRGVLVEDKRE----IAKRYLSSHFIIDILAVLPLPQMVILIIIPHMRGSSSL 220

Query: 258 MILRSLQFVLVIQHLPRALRIYKFLKEVRLSRSLFPNSTVGNAIFNLFLYMLSSHAFGAF 317
                L+F++  Q++PR +RIY   KEV  +  +   +    A FNLFLYML+SH FGAF
Sbjct: 221 NTKNMLKFIVFFQYIPRFIRIYPLYKEVTRTSGILTETAWAGAAFNLFLYMLASHVFGAF 280

Query: 318 WYLFAIERKASCLQKRC-GTDPYCPRIHNLSSVERSCIKDCSANASSNDK--------TA 377
           WYLF+IER+  C ++ C   +P C     +S +     +    NA  N+         T 
Sbjct: 281 WYLFSIERETVCWKQACERNNPPC-----ISKLLYCDPETAGGNAFLNESCPIQTPNTTL 340

Query: 378 FNFGIFDDALTSGVVYSTDLICKLCYCCWWGLQNLSSLGQGLKTSQHIWEISFAVSITIA 437
           F+FGIF DAL SGVV S D   K  YC WWGLQNLSSLGQ LKTS +IWEI FAV I+IA
Sbjct: 341 FDFGIFLDALQSGVVESQDFPQKFFYCFWWGLQNLSSLGQNLKTSTYIWEICFAVFISIA 400

Query: 438 GLVLFALLIGNLQTFLQANIARLEELRSKVEDIELWMPSILSRVNQGSKSNNVSMFQILN 497
           GLVLF+ LIGN+QT+LQ+   RLEE+R K  D E WM   L   N   +      ++   
Sbjct: 401 GLVLFSFLIGNMQTYLQSTTTRLEEMRVKRRDAEQWMSHRLLPENLRKRIRRYEQYKWQE 460

Query: 498 GR-----------------------------------QLNEKLIDAVCGYLKPVVYTERN 557
            R                                   +++E+L+DA+C  L+PV+YTE +
Sbjct: 461 TRGVDEENLLSNLPKDLRRDIKRHLCLALLMRVPMFEKMDEQLLDALCDRLQPVLYTEES 520

Query: 558 IIVVKGEPLDKMIFVIHGKLWMYSNDNREDGTIGSSESLTKDNYFGEGLLNWGLKDPLLS 617
            IV +G+P+D+M+F++ GKL   + +    G + +SE L   ++ GE LL W L     S
Sbjct: 521 YIVREGDPVDEMLFIMRGKLLTITTNGGRTGFL-NSEYLGAGDFCGEELLTWALDPHSSS 580

Query: 618 TVPLSPKTVSAHTKVEAFVLSANDLKIFISKFWWLISPELRNHPK-FEEQCKKWAVPLMC 674
            +P+S +TV A  +VEAF L A+DLK   S+F  L S +LR+  + + +Q K WA   + 
Sbjct: 581 NLPISTRTVRALMEVEAFALKADDLKFVASQFRRLHSKQLRHTFRYYSQQWKTWAACFIQ 640

BLAST of Cp4.1LG14g06530 vs. TAIR 10
Match: AT1G01340.1 (cyclic nucleotide gated channel 10 )

HSP 1 Score: 326.6 bits (836), Expect = 4.7e-89
Identity = 211/563 (37.48%), Postives = 303/563 (53.82%), Query Frame = 0

Query: 124 LHVWNEVVVILCVAAILLDPLFCYILVVDEKKICIEFDQKLRITAIVLRSLIDFGYILVI 183
           L  WN++ +  CV A+ +DPLF YI +VD  + C+  D KL I A +LR+LID  YI+ I
Sbjct: 71  LQNWNKIFLFACVVALAIDPLFFYIPIVDSARHCLTLDSKLEIAASLLRTLIDAFYIIHI 130

Query: 184 AFHFRMGYTGPY-----------DAKRETLYKIGRRYLLSNFTVHILAVLPLPQVVNLLV 243
            F FR  Y  P            DAK      I  +YL S F + +L++LPLPQ+V L V
Sbjct: 131 VFQFRTAYIAPSSRVFGRGELVDDAK-----AIALKYLSSYFIIDLLSILPLPQIVVLAV 190

Query: 244 IPAAKGSHFSMILRSLQFVLVIQHLPRALRIYKFLKEVRLSRSLFPNSTVGNAIFNLFLY 303
           IP+       +    L+F ++ Q++PR LR+Y    EV  +  +   +    A +NL LY
Sbjct: 191 IPSVNQPVSLLTKDYLKFSIIAQYVPRILRMYPLYTEVTRTSGIVTETAWAGAAWNLSLY 250

Query: 304 MLSSHAFGAFWYLFAIERKASCLQKRCGTDP-------YCPRIHNLSSVERSCIKDCSAN 363
           ML+SH FGA WYL ++ER+  C Q+ C           YC    N+S+   +        
Sbjct: 251 MLASHVFGALWYLISVEREDRCWQEACEKTKGCNMKFLYCENDRNVSNNFLTTSCPFLDP 310

Query: 364 ASSNDKTAFNFGIFDDALTSGVVYSTDLICKLCYCCWWGLQNLSSLGQGLKTSQHIWEIS 423
               + T FNFGIF DAL SGVV S D   K  YC WWGL+NLS+LGQ L+TS+ + EI 
Sbjct: 311 GDITNSTIFNFGIFTDALKSGVVESHDFWKKFFYCFWWGLRNLSALGQNLQTSKFVGEII 370

Query: 424 FAVSITIAGLVLFALLIGNLQTFLQANIARLEELRSKVEDIELWM-----PSILSR---- 483
           FA+SI I+GLVLFALLIGN+Q +L++   R EE+R +  D E WM     P  L +    
Sbjct: 371 FAISICISGLVLFALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPEDLRKRIRR 430

Query: 484 -----------VNQGSKSNN--------------------VSMFQILNGRQLNEKLIDAV 543
                      V + +   N                    V +F+I     ++E+L+DAV
Sbjct: 431 YEQYRWQETRGVEEETLLRNLPKDLRRDIKRHLCLDLLKKVPLFEI-----MDEQLLDAV 490

Query: 544 CGYLKPVVYTERNIIVVKGEPLDKMIFVIHGKLWMYSNDNREDGTIGSSESLTKDNYFGE 603
           C  L+PV+YTE + ++ +G+P+ +M+FV+ G+L   + +    G   ++ +L   ++ GE
Sbjct: 491 CDRLRPVLYTENSYVIREGDPVGEMLFVMRGRLVSATTNGGRSGFF-NAVNLKASDFCGE 550

Query: 604 GLLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDLKIFISKFWWLISPELRNHPKF- 628
            LL W L     S  P+S +TV A T+VEAF L+A DLK   S+F  L S +L++  +F 
Sbjct: 551 DLLPWALDPQSSSHFPISTRTVQALTEVEAFALTAEDLKSVASQFRRLHSKQLQHTFRFY 610

BLAST of Cp4.1LG14g06530 vs. TAIR 10
Match: AT1G01340.2 (cyclic nucleotide gated channel 10 )

HSP 1 Score: 326.6 bits (836), Expect = 4.7e-89
Identity = 211/563 (37.48%), Postives = 303/563 (53.82%), Query Frame = 0

Query: 124 LHVWNEVVVILCVAAILLDPLFCYILVVDEKKICIEFDQKLRITAIVLRSLIDFGYILVI 183
           L  WN++ +  CV A+ +DPLF YI +VD  + C+  D KL I A +LR+LID  YI+ I
Sbjct: 76  LQNWNKIFLFACVVALAIDPLFFYIPIVDSARHCLTLDSKLEIAASLLRTLIDAFYIIHI 135

Query: 184 AFHFRMGYTGPY-----------DAKRETLYKIGRRYLLSNFTVHILAVLPLPQVVNLLV 243
            F FR  Y  P            DAK      I  +YL S F + +L++LPLPQ+V L V
Sbjct: 136 VFQFRTAYIAPSSRVFGRGELVDDAK-----AIALKYLSSYFIIDLLSILPLPQIVVLAV 195

Query: 244 IPAAKGSHFSMILRSLQFVLVIQHLPRALRIYKFLKEVRLSRSLFPNSTVGNAIFNLFLY 303
           IP+       +    L+F ++ Q++PR LR+Y    EV  +  +   +    A +NL LY
Sbjct: 196 IPSVNQPVSLLTKDYLKFSIIAQYVPRILRMYPLYTEVTRTSGIVTETAWAGAAWNLSLY 255

Query: 304 MLSSHAFGAFWYLFAIERKASCLQKRCGTDP-------YCPRIHNLSSVERSCIKDCSAN 363
           ML+SH FGA WYL ++ER+  C Q+ C           YC    N+S+   +        
Sbjct: 256 MLASHVFGALWYLISVEREDRCWQEACEKTKGCNMKFLYCENDRNVSNNFLTTSCPFLDP 315

Query: 364 ASSNDKTAFNFGIFDDALTSGVVYSTDLICKLCYCCWWGLQNLSSLGQGLKTSQHIWEIS 423
               + T FNFGIF DAL SGVV S D   K  YC WWGL+NLS+LGQ L+TS+ + EI 
Sbjct: 316 GDITNSTIFNFGIFTDALKSGVVESHDFWKKFFYCFWWGLRNLSALGQNLQTSKFVGEII 375

Query: 424 FAVSITIAGLVLFALLIGNLQTFLQANIARLEELRSKVEDIELWM-----PSILSR---- 483
           FA+SI I+GLVLFALLIGN+Q +L++   R EE+R +  D E WM     P  L +    
Sbjct: 376 FAISICISGLVLFALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPEDLRKRIRR 435

Query: 484 -----------VNQGSKSNN--------------------VSMFQILNGRQLNEKLIDAV 543
                      V + +   N                    V +F+I     ++E+L+DAV
Sbjct: 436 YEQYRWQETRGVEEETLLRNLPKDLRRDIKRHLCLDLLKKVPLFEI-----MDEQLLDAV 495

Query: 544 CGYLKPVVYTERNIIVVKGEPLDKMIFVIHGKLWMYSNDNREDGTIGSSESLTKDNYFGE 603
           C  L+PV+YTE + ++ +G+P+ +M+FV+ G+L   + +    G   ++ +L   ++ GE
Sbjct: 496 CDRLRPVLYTENSYVIREGDPVGEMLFVMRGRLVSATTNGGRSGFF-NAVNLKASDFCGE 555

Query: 604 GLLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDLKIFISKFWWLISPELRNHPKF- 628
            LL W L     S  P+S +TV A T+VEAF L+A DLK   S+F  L S +L++  +F 
Sbjct: 556 DLLPWALDPQSSSHFPISTRTVQALTEVEAFALTAEDLKSVASQFRRLHSKQLQHTFRFY 615

BLAST of Cp4.1LG14g06530 vs. TAIR 10
Match: AT1G19780.1 (cyclic nucleotide gated channel 8 )

HSP 1 Score: 323.2 bits (827), Expect = 5.2e-88
Identity = 202/563 (35.88%), Postives = 296/563 (52.58%), Query Frame = 0

Query: 124 LHVWNEVVVILCVAAILLDPLFCYILVVDEKKICIEFDQKLRITAIVLRSLIDFGYILVI 183
           L +WN + VI C+ A+ +DPLF Y+ +VD  K CI  D KL +T   LR++ID  Y+  +
Sbjct: 105 LLLWNRMFVISCILAVSVDPLFFYLPIVDNSKNCIGIDSKLAVTTTTLRTIIDVFYLTRM 164

Query: 184 AFHFRMGYTGPYD---AKRETLY---KIGRRYLLSNFTVHILAVLPLPQVVNLLVIPAAK 243
           A  FR  Y  P      + E +    KI  RYL   F V  LAVLPLPQ+     +  +K
Sbjct: 165 ALQFRTAYIAPSSRVFGRGELVIDPAKIAERYLTRYFIVDFLAVLPLPQIAVWKFLHGSK 224

Query: 244 GSHFSMILRSLQFVLVIQHLPRALRIYKFLKEVRLSRSLFPNSTVGNAIFNLFLYMLSSH 303
           G+      ++L  +++ Q++PR +R      E++ +   F       A + L  YML+SH
Sbjct: 225 GTDVLPTKQALLHIVITQYIPRFVRFIPLTSELKKTAGAFAEGAWAGAAYYLLWYMLASH 284

Query: 304 AFGAFWYLFAIERKASCLQKRCGTDP---------YCPRIHNLSSVERSCIK-------- 363
             GAFWY+ ++ER  +CL+  C   P         YC     +SS E   IK        
Sbjct: 285 ITGAFWYMLSVERNDTCLRSACKVQPDPKVCVQILYCGS-KLMSSRETDWIKSVPDLFKN 344

Query: 364 DCSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLCYCCWWGLQNLSSLGQGLKTSQH 423
           +CSA    +D++ FN+GI+  A++SG+V ST    K CYC WWGLQNLS+LGQGL+TS +
Sbjct: 345 NCSA---KSDESKFNYGIYSQAVSSGIVSSTTFFSKFCYCLWWGLQNLSTLGQGLQTSTY 404

Query: 424 IWEISFAVSITIAGLVLFALLIGNLQTFLQANIARLEELRSKVEDIELWMPSILSRVNQG 483
             E+ F+++I +AGL+LFALLIGN+QT+LQ+   RLEE+R K  D E WM       N  
Sbjct: 405 PGEVLFSIAIAVAGLLLFALLIGNMQTYLQSLTVRLEEMRIKRRDSEQWMHHRSLPQNLR 464

Query: 484 SKSNNVSMFQILNGR-----------------------------------QLNEKLIDAV 543
            +      ++ L  R                                    ++E+L+DA+
Sbjct: 465 ERVRRYDQYKWLETRGVDEENIVQSLPKDLRRDIKRHLCLNLVRRVPLFANMDERLLDAI 524

Query: 544 CGYLKPVVYTERNIIVVKGEPLDKMIFVIHGKLWMYSNDNREDGTIGSSESLTKDNYFGE 603
           C  LKP +YTE   IV +G+P+++M+F+I G+L   + D    G       L + ++ GE
Sbjct: 525 CERLKPSLYTESTYIVREGDPVNEMLFIIRGRLESVTTDGGRSGFFNRG-LLKEGDFCGE 584

Query: 604 GLLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDLKIFISKFWWLISPELRNHPKF- 628
            LL W L     S +P S +TV A T+VEAF L A +LK   S+F  L S +++   +F 
Sbjct: 585 ELLTWALDPKAGSNLPSSTRTVKALTEVEAFALEAEELKFVASQFRRLHSRQVQQTFRFY 644

BLAST of Cp4.1LG14g06530 vs. TAIR 10
Match: AT5G57940.1 (cyclic nucleotide gated channel 5 )

HSP 1 Score: 317.8 bits (813), Expect = 2.2e-86
Identity = 221/660 (33.48%), Postives = 341/660 (51.67%), Query Frame = 0

Query: 79  DLSCDLFRSQSCSREHVAERPSGKIH----------CLKKGM---SKGLRFIKKCIG--- 138
           DL   L  S    +++ A   SG++H            KKG+   SKGL+ I + +G   
Sbjct: 13  DLDSRLPSSSVAFQQNYASNFSGQLHPIHASNETSRSFKKGIQKGSKGLKSIGRSLGFGV 72

Query: 139 -------ELHV---------------WNEVVVILCVAAILLDPLFCYILVVDEKKICIEF 198
                  +L V                N++ V  C+ ++ +DP F Y+ V++ +  C+  
Sbjct: 73  YRAVFPEDLKVSEKKIFDPQDKFLLYCNKLFVASCILSVFVDPFFFYLPVINAESKCLGI 132

Query: 199 DQKLRITAIVLRSLIDFGYILVIAFHFRMGYTGPYD---AKRETLY---KIGRRYLLSNF 258
           D+KL ITA  LR+ ID  Y+  +A   R  Y  P      + E +    +I +RYL   F
Sbjct: 133 DRKLAITASTLRTFIDVFYLAHMALQLRTAYIAPSSRVFGRGELVIDPAQIAKRYLQRWF 192

Query: 259 TVHILAVLPLPQVVNLLVIPAAKGSHFSMILRSLQFVLVIQHLPRALRIYKFLKEVRLSR 318
            +  L+VLPLPQ+V    + ++ GS      ++L F++++Q++PR LR+     E++ + 
Sbjct: 193 IIDFLSVLPLPQIVVWRFLQSSNGSDVLATKQALLFIVLVQYIPRFLRVLPLTSELKRTA 252

Query: 319 SLFPNSTVGNAIFNLFLYMLSSHAFGAFWYLFAIERKASCLQK------RCGTD-PYCPR 378
            +F  +    A + L LYML+SH  GAFWYL A+ER  +C Q+       C TD  YC  
Sbjct: 253 GVFAETAWAGAAYYLLLYMLASHIVGAFWYLLALERNDACWQEACIDAGNCSTDFLYCGN 312

Query: 379 IHNL------SSVERSCIKD-CSANASSNDKTAFNFGIFDDALTSGVVYSTDLICKLCYC 438
             N+      +  + S +K  C A+   N+   F+FGI+  AL+SG+V S + I K CYC
Sbjct: 313 -QNMDGYAVWNRAKESVLKSKCRADLDDNN-PPFDFGIYTQALSSGIVSSQNFIVKYCYC 372

Query: 439 CWWGLQNLSSLGQGLKTSQHIWEISFAVSITIAGLVLFALLIGNLQTFLQANIARLEELR 498
            WWGLQNLS+LGQGL+TS +  EI F++S+ I+GL+LFALLIGN+QT+LQ+   RLEE+R
Sbjct: 373 LWWGLQNLSTLGQGLETSTYPMEIIFSISLAISGLILFALLIGNMQTYLQSLTIRLEEMR 432

Query: 499 SKVEDIELWMPSILSRVNQGSKSNNVSMFQILNGR------------------------- 558
            K  D E WM   +   +   +      ++ L  R                         
Sbjct: 433 VKRRDSEQWMHHRMLPQDLRERVRRYDQYKWLETRGVDEEYLVQNLPKDLRRDIKRHLCL 492

Query: 559 ----------QLNEKLIDAVCGYLKPVVYTERNIIVVKGEPLDKMIFVIHGKLWMYSNDN 618
                      +++KL+DA+C  LKP ++TE   +V +G+P+D+M+F+I G+L   + D 
Sbjct: 493 ALVRRVPLFKSMDDKLLDAICMRLKPCLFTESTYLVREGDPVDEMLFIIRGRLESVTTDG 552

Query: 619 REDGTIGSSESLTKDNYF-GEGLLNWGLKDPLLSTVPLSPKTVSAHTKVEAFVLSANDLK 644
              G    + SL K+  F GE LL W L       +P S +TV A T+VEAF L++ +LK
Sbjct: 553 GRSGFF--NRSLLKEGEFCGEELLTWALDPKSGVNLPSSTRTVKALTEVEAFALTSEELK 612